Affinage

FSCN1

Fascin · UniProt Q16658

Round 2 corrected
Length
493 aa
Mass
54.5 kDa
Annotated
2026-04-28
130 papers in source corpus 19 papers cited in narrative 19 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

FSCN1 (Fascin-1) is an actin-bundling protein that crosslinks filamentous actin into parallel bundles, driving the formation of filopodia, microspikes, and invadopodia to promote cell migration and invasion (PMID:9571235, PMID:20137952). Its actin-bundling activity is negatively regulated by PKC-mediated phosphorylation at Ser39, which controls fascin dynamics at invadopodia, and can be pharmacologically inhibited by migrastatin analogues that occupy an actin-binding site, as defined by X-ray crystallography (PMID:20137952, PMID:20393565). FSCN1 transcription is activated by β-catenin–TCF signaling in colorectal cancer and by GRHL3 during wound reepithelialization, where fascin promotes collective keratinocyte migration by downregulating E-cadherin at adherens junctions (PMID:17638895, PMID:34494554). Post-transcriptionally, FSCN1 is repressed by multiple tumor-suppressive microRNAs—including miR-145, miR-133a, and miR-133b—that directly bind its 3′UTR, and its mRNA is stabilized by the RNA-binding protein HuR, which is competitively sequestered by the lncRNA ADORA2A-AS1 (PMID:20160723, PMID:21351259, PMID:34733789).

Mechanistic history

Synthesis pass · year-by-year structured walk · 9 steps
  1. 1998 High

    Establishing that fascin directly bundles actin filaments and is sufficient to reorganize the cortical cytoskeleton and dramatically enhance cell migration resolved the basic molecular activity of FSCN1.

    Evidence Microinjection of purified fascin protein and overexpression in epithelial cells with Boyden chamber migration assays

    PMID:9571235

    Open questions at the time
    • Regulatory mechanisms controlling fascin bundling activity were unknown
    • Whether fascin-driven migration contributed to pathological invasion was not tested
  2. 2007 High

    Identifying FSCN1 as a direct β-catenin–TCF transcriptional target placed it within Wnt pathway-driven carcinogenesis and showed that fascin expression at tumor invasive fronts functionally promotes metastasis in vivo.

    Evidence TCF reporter assays, siRNA knockdown, overexpression, and xenograft metastasis models in colorectal cancer

    PMID:17638895

    Open questions at the time
    • Whether other transcription factors independently activate FSCN1 in non-Wnt contexts was unknown
    • Post-translational regulation of fascin at the invasive front was not addressed
  3. 2010 High

    Demonstrating that PKC phosphorylation at Ser39 regulates fascin localization and function at invadopodia, and that migrastatin analogues directly block fascin's actin-binding site (crystal structure resolved), established both the key regulatory switch and a pharmacological strategy to inhibit fascin-dependent invasion.

    Evidence Phosphomutant constructs (S39A/S39D) with live-cell invadopodia imaging and 3D invasion assays; X-ray crystallography of fascin–macroketone complex with in vivo metastasis inhibition

    PMID:20137952 PMID:20393565

    Open questions at the time
    • Whether Ser39 phosphorylation is the sole regulatory post-translational modification was unclear
    • Clinical applicability of migrastatin analogues was not established
  4. 2010 High

    Discovery that miR-145 and miR-133a/b directly bind the FSCN1 3′UTR to repress expression across multiple tumor types established a major post-transcriptional regulatory layer governing fascin abundance.

    Evidence Luciferase 3′UTR reporter assays, miRNA mimic transfection, and phenocopy by FSCN1 siRNA in bladder and esophageal carcinoma cells

    PMID:20160723 PMID:21351259

    Open questions at the time
    • Relative contribution of individual miRNAs versus combinatorial repression was not quantified
    • Upstream mechanisms silencing these miRNAs in tumors were only partially known
  5. 2017 Medium

    Placing FSCN1 downstream of EGF–MAPK signaling in triple-negative breast cancer extended the transcriptional regulation map beyond Wnt to receptor tyrosine kinase pathways.

    Evidence EGFR inhibitor (Gefitinib) treatment combined with FSCN1 siRNA in TNBC cells

    PMID:29142206

    Open questions at the time
    • Direct transcription factor mediating MAPK-to-FSCN1 induction was not identified
    • Single-lab study without genetic epistasis
  6. 2018 Medium

    Showing that miR-145-5p promoter hypermethylation in laryngeal carcinoma relieves FSCN1 repression and promotes EMT connected epigenetic deregulation to fascin-driven invasion.

    Evidence Promoter methylation analysis, luciferase reporter assay, gain/loss-of-function studies, in vivo tumor growth

    PMID:30341010

    Open questions at the time
    • Whether DNA methylation of miR-145 is a general mechanism across FSCN1-high tumors was not tested
    • E-cadherin loss was inferred but not mechanistically dissected downstream of fascin
  7. 2021 High

    Demonstrating that GRHL3 directly opens chromatin at the Fscn1 locus to drive transcription during wound reepithelialization, and that fascin then downregulates E-cadherin to loosen adherens junctions, established a physiological (non-oncogenic) role for fascin in collective cell migration.

    Evidence ATAC-seq on wounded keratinocytes, Grhl3 conditional knockout mice, wound closure assays, E-cadherin immunofluorescence

    PMID:34494554

    Open questions at the time
    • Mechanism by which fascin downregulates E-cadherin (transcriptional vs. post-translational) was not resolved
    • Whether GRHL3–FSCN1 axis operates in other epithelial repair contexts is unknown
  8. 2021 Medium

    Identification that HuR binds and stabilizes FSCN1 mRNA, and that the lncRNA ADORA2A-AS1 competitively sequesters HuR to reduce FSCN1, revealed an RNA-binding-protein layer of post-transcriptional control complementary to miRNA repression.

    Evidence RNA immunoprecipitation, RNA pull-down, mRNA stability assays in hepatocellular carcinoma cells and xenograft models

    PMID:34733789

    Open questions at the time
    • Whether HuR-mediated stabilization is the dominant mRNA-level control in non-hepatic contexts is untested
    • Quantitative contribution of HuR versus miRNA pathways to FSCN1 protein levels not compared
  9. 2023 Medium

    Discovery that p300-mediated acetylation of SF3A2 controls alternative splicing to regulate FSCN1 expression in cardiomyocytes broadened fascin's functional context to cardiac ischemic protection and mitochondrial respiration.

    Evidence Acetylomics, SPR binding assay, site-specific mutagenesis of SF3A2-K10, RNA-seq, in vivo cardiac ischemia model

    PMID:38101749

    Open questions at the time
    • Specific splicing event in FSCN1 controlled by SF3A2 acetylation status was not fully characterized
    • Whether fascin has direct roles in mitochondrial function or acts indirectly through cytoskeletal remodeling is unresolved
    • Single-lab finding not yet independently replicated

Open questions

Synthesis pass · forward-looking unresolved questions
  • The mechanism by which fascin downregulates E-cadherin, the full set of post-translational modifications beyond Ser39, the structural basis for actin-bundling geometry in cellulo, and the physiological relevance of fascin in non-migratory contexts (e.g., cardiac protection) remain open questions.
  • No comprehensive post-translational modification map beyond Ser39 phosphorylation
  • In vivo structural characterization of fascin-bundled actin arrays is lacking
  • Cardiac and neuronal roles of fascin are mechanistically uncharacterized

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 3 GO:0008092 cytoskeletal protein binding 3
Localization
GO:0005856 cytoskeleton 2 GO:0005886 plasma membrane 2 GO:0005829 cytosol 1
Pathway
R-HSA-1643685 Disease 4 R-HSA-162582 Signal Transduction 3 R-HSA-1500931 Cell-Cell communication 1
Partners
CTNNB1ELAVL1GRHL3LYARSOX2

Evidence

Reading pass · 19 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 Fascin (FSCN1) directly bundles actin filaments and, when expressed in epithelial cells (LLC-PK1), induces membrane protrusions (lamellipodia, microvilli), reorganizes the microfilament cytoskeleton at the cell periphery, and increases cell migration 8–17-fold in a Boyden chamber assay. Microinjection of purified fascin protein into cells recapitulated these morphological changes, establishing direct causality. Overexpression in epithelial cells, microinjection of purified fascin protein, Boyden chamber migration assay, immunofluorescence Molecular biology of the cell High 9571235
2007 FSCN1 (fascin1) is a direct transcriptional target of β-catenin–TCF signaling in colorectal cancer cells. Forced expression of fascin1 increased cell migration and invasion in vitro and caused cell dissemination and metastasis in vivo; suppression by siRNA reduced invasion. Fascin1 was exclusively localized at the invasive front of tumors displaying nuclear β-catenin. TCF reporter assays, siRNA knockdown, overexpression, Boyden chamber invasion, in vivo xenograft/metastasis model, immunohistochemistry Cancer research High 17638895
2010 Fascin (FSCN1) is an integral component of invadopodia in cancer cells and stabilizes actin within invadopodia. Phosphorylation of fascin at serine 39 by PKC regulates its localization and activity at invadopodia. Fascin promotes invasive migration into collagen I–Matrigel 3D matrices, particularly in elongated mesenchymal motility. siRNA knockdown, phosphomutant constructs (S39A/S39D), live-cell imaging, 3D invasion assay, immunofluorescence of invadopodia markers Current biology : CB High 20137952
2010 Migrastatin analogues (e.g., macroketone) directly bind fascin (FSCN1) at one of its actin-binding sites and inhibit its actin-bundling activity, thereby blocking tumor cell migration, invasion, and metastasis. X-ray crystal structure of fascin–macroketone complex defined the binding site. X-ray crystallography, in vitro actin-bundling assay, cell migration/invasion assays, in vivo metastasis mouse model Nature High 20393565
2005 FSCN1 was identified as part of a gene set that mediates breast cancer metastasis specifically to the lung, established through in vivo selection of metastatic subpopulations and functional verification. In vivo selection, transcriptomic analysis, functional verification in xenograft models Nature Medium 16049480
2010 miR-145 and miR-133a directly bind to the 3′UTR of FSCN1 mRNA and repress FSCN1 mRNA and protein expression in bladder cancer cells, functioning as tumor suppressors. Luciferase reporter assay confirmed direct binding. siRNA knockdown of FSCN1 phenocopied miR-145/133a overexpression, inhibiting cell viability, wound healing, and invasion. Luciferase reporter assay, transfection of miRNA mimics, siRNA knockdown, cell growth/wound-healing/invasion assays, immunohistochemistry, in situ hybridization British journal of cancer High 20160723
2010 miR-145, miR-133a, and miR-133b directly regulate FSCN1 expression in esophageal squamous cell carcinoma (ESCC) by binding conserved target sites in the 3′UTR of FSCN1. Luciferase reporter assays confirmed two miR-145 sites and one miR-133a/b site. FSCN1 loss-of-function inhibited cell growth and invasion in ESCC. Luciferase reporter assay, miRNA gain-of-function transfection, siRNA knockdown, cell proliferation/invasion assays International journal of cancer High 21351259
2013 miR-133a directly targets FSCN1 mRNA in esophageal squamous cell carcinoma (ESCC); transfection of miR-133a mimic decreased both FSCN1 mRNA and protein levels and inhibited proliferation and invasion. FSCN1 and MMP14 are co-regulated invadopodia-related proteins whose combined expression correlates with worst prognosis. miRNA mimic transfection, qPCR, western blot, siRNA knockdown, proliferation/invasion assays, immunohistochemistry British journal of cancer Medium 24196787
2013 miR-133a directly targets FSCN1 in pancreatic cancer, confirmed by luciferase reporter assay. Transfection of miR-133a mimics in PANC-1 cells reduced FSCN1 protein, and decreased cell proliferation, invasion, and migration while promoting apoptosis in vitro, and suppressed tumorigenicity in vivo. Luciferase reporter assay, miRNA mimic transfection, siRNA knockdown, proliferation/invasion/migration assays, xenograft mouse model Oncology research Medium 25198665
2017 EGF induces FSCN1 expression through activation of the MAPK pathway in triple-negative breast cancer (TNBC) cells, which subsequently promotes cell migration and invasion. Co-treatment with FSCN1 siRNA and the EGFR inhibitor Gefitinib produced greater reduction of FSCN1 expression than either treatment alone. siRNA knockdown, EGFR inhibitor (Gefitinib) treatment, western blot, migration/invasion assays Scientific reports Medium 29142206
2018 miR-145-5p directly targets and negatively regulates FSCN1 via its 3′UTR (confirmed by luciferase assay) in laryngeal squamous cell carcinoma. Low miR-145-5p/high FSCN1 promotes EMT, cell migration, invasion, and growth. The miR-145-5p promoter is hypermethylated in LSCC, repressing miR-145-5p and thus relieving FSCN1 repression. Luciferase reporter assay, gain/loss-of-function studies, promoter methylation analysis, siRNA, in vivo tumor growth assay Molecular therapy Medium 30341010
2019 YAP1 transcriptionally activates LINC00152, which acts as a ceRNA sponging miR-632 and miR-185-3p to upregulate FSCN1 expression in colorectal cancer, promoting proliferation and metastasis. This YAP1/LINC00152/miR-632|miR-185-3p/FSCN1 axis was established by in vitro and in vivo experiments. ceRNA reporter assays, miRNA sponge assays, siRNA/shRNA knockdown, xenograft mouse models Advanced science Medium 32042551
2015 FSCN1 knockdown in pituitary adenoma (GH3) cells suppressed cell invasion through transwell assay and downregulated Notch1 and DLL3 expression, placing FSCN1 upstream of Notch signaling in pituitary adenoma invasiveness. siRNA knockdown, transwell invasion assay, western blot for Notch1/DLL3 Molecular and cellular endocrinology Low 26522130
2020 SOX2 promotes brain metastasis of breast cancer by transcriptionally upregulating FSCN1 (and HBEGF) through activation of AKT and β-catenin signaling pathways, thereby increasing cancer cell adhesion to brain microvascular endothelial cells and transendothelial migration across the blood–brain barrier. SOX2 overexpression/silencing, in vitro BBB migration assay, AKT/β-catenin pathway inhibitors, xenograft brain metastasis model, mRNA profiling Molecular therapy oncolytics Medium 32322668
2021 The transcription factor GRHL3 directly activates FSCN1 transcription during wound reepithelialization by increasing chromatin accessibility near the Fscn1 gene (ATAC-seq shows enriched GRHL3 motifs). FSCN1 (Fascin-1) in turn downregulates E-cadherin to relax adherens junctions between suprabasal keratinocytes, promoting collective migration. Grhl3-cKO mice have lower FSCN1 expression at wound fronts and impaired wound closure. ATAC-seq on wounded keratinocytes, conditional knockout mice (Grhl3-cKO), immunofluorescence for E-cadherin and FSCN1, wound closure assays JCI insight High 34494554
2021 LYAR (a nucleolar protein) directly binds the FSCN1 promoter (shown by ChIP assay) and transcriptionally activates FSCN1 expression. FSCN1 is a downstream effector of LYAR-promoted CRC cell migration and invasion, and FSCN1 knockdown inhibits subcutaneous tumorigenesis and downregulates FASN and SCD (fatty acid synthesis genes). ChIP assay, gene reporter assay, microarray, siRNA/shRNA knockdown, xenograft tumor model Oxidative medicine and cellular longevity Medium 35069968
2021 ADORA2A-AS1 (lncRNA) competitively binds HuR (RNA-binding protein), preventing HuR from binding and stabilizing FSCN1 transcript, thereby decreasing FSCN1 mRNA stability and expression. Decreased FSCN1 in turn suppresses AKT pathway activation in hepatocellular carcinoma. RNA pull-down and RIP confirmed ADORA2A-AS1–HuR–FSCN1 interactions. RNA pull-down, RNA immunoprecipitation (RIP), RNA stability assay, qRT-PCR, western blot, xenograft and metastasis models Frontiers in oncology Medium 34733789
2023 Ginsenoside Rb2 inhibits p300 acetyltransferase activity (confirmed by surface plasmon resonance and enzymatic assays showing direct binding), thereby reducing p300-mediated acetylation of SF3A2 at lysine 10. Decreased SF3A2-K10 acetylation promotes alternative splicing that upregulates FSCN1 expression in cardiomyocytes, enhancing mitochondrial respiration and protecting against ischemic injury. 4D-label-free acetylomics, co-immunoprecipitation, surface plasmon resonance, cellular thermal shift assay, site-specific mutagenesis, RNA-seq, shRNA interference, in vivo cardiac ischemia model Journal of advanced research Medium 38101749
2006 Fascin expression in breast carcinomas is strongly associated with the basal-like phenotype and is highly expressed in BRCA1-associated tumors (83.3%), establishing a link between FSCN1 and the basal-like/BRCA1 tumor subtype. However, fascin expression per se was not independently associated with metastatic behavior in node-negative sporadic breast cancers. Tissue microarray immunohistochemistry of 230 sporadic and 28 hereditary node-negative invasive breast carcinomas Clinical cancer research Low 16533778

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2005 Genes that mediate breast cancer metastasis to lung. Nature 2358 16049480
2012 Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell 1718 22658674
2005 A human protein-protein interaction network: a resource for annotating the proteome. Cell 1704 16169070
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2016 ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure. Cell 1233 26777405
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2007 Large-scale mapping of human protein-protein interactions by mass spectrometry. Molecular systems biology 733 17353931
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
1998 TNF receptor-deficient mice reveal divergent roles for p55 and p75 in several models of inflammation. Journal of immunology (Baltimore, Md. : 1950) 565 9551933
2021 Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV. Nature 532 33845483
2011 Mapping the NPHP-JBTS-MKS protein network reveals ciliopathy disease genes and pathways. Cell 507 21565611
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2010 Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science (New York, N.Y.) 421 20360068
2010 miR-145, miR-133a and miR-133b: Tumor-suppressive miRNAs target FSCN1 in esophageal squamous cell carcinoma. International journal of cancer 409 21351259
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
1996 Tumor necrosis factor (TNF)-alpha inhibits insulin signaling through stimulation of the p55 TNF receptor and activation of sphingomyelinase. The Journal of biological chemistry 348 8662983
2010 miR-145 and miR-133a function as tumour suppressors and directly regulate FSCN1 expression in bladder cancer. British journal of cancer 306 20160723
2006 Prognostic significance of basal-like phenotype and fascin expression in node-negative invasive breast carcinomas. Clinical cancer research : an official journal of the American Association for Cancer Research 264 16533778
2011 A directed protein interaction network for investigating intracellular signal transduction. Science signaling 258 21900206
2022 Tau interactome maps synaptic and mitochondrial processes associated with neurodegeneration. Cell 256 35063084
2010 Identification of KIAA1018/FAN1, a DNA repair nuclease recruited to DNA damage by monoubiquitinated FANCD2. Cell 250 20603015
2010 The actin-bundling protein fascin stabilizes actin in invadopodia and potentiates protrusive invasion. Current biology : CB 249 20137952
2007 Fascin, a novel target of beta-catenin-TCF signaling, is expressed at the invasive front of human colon cancer. Cancer research 242 17638895
2013 Induction of IL-17 and nonclassical T-cell activation by HIV-Tat protein. Proceedings of the National Academy of Sciences of the United States of America 235 23898208
1997 Lipopolysaccharide-stimulated osteoclastogenesis is mediated by tumor necrosis factor via its P55 receptor. The Journal of clinical investigation 235 9294124
2010 Migrastatin analogues target fascin to block tumour metastasis. Nature 221 20393565
2010 MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis. Immunology and cell biology 221 20458337
1998 Fascin, an actin-bundling protein, induces membrane protrusions and increases cell motility of epithelial cells. Molecular biology of the cell 220 9571235
2015 ∆F508 CFTR interactome remodelling promotes rescue of cystic fibrosis. Nature 209 26618866
2009 Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6. Science signaling 207 19738201
2000 Caspase activities and tumor necrosis factor receptor R1 (p55) level are elevated in the substantia nigra from parkinsonian brain. Journal of neural transmission (Vienna, Austria : 1996) 206 10821442
1994 Fas antigen and p55 TNF receptor signal apoptosis through distinct pathways. Journal of immunology (Baltimore, Md. : 1950) 202 7509828
1993 Human TNF mutants with selective activity on the p55 receptor. Nature 186 8380906
1999 Inhibition of receptor internalization by monodansylcadaverine selectively blocks p55 tumor necrosis factor receptor death domain signaling. The Journal of biological chemistry 175 10187805
1994 In vitro binding studies suggest a membrane-associated complex between erythroid p55, protein 4.1, and glycophorin C. The Journal of biological chemistry 151 8132590
2000 LPS-induced liver injury in D-galactosamine-sensitized mice requires secreted TNF-alpha and the TNF-p55 receptor. American journal of physiology. Regulatory, integrative and comparative physiology 147 10801288
1999 Roles for tumor necrosis factor receptor p55 and sphingomyelinase in repairing the cutaneous permeability barrier. The Journal of clinical investigation 139 10606630
2019 Targeting YAP1/LINC00152/FSCN1 Signaling Axis Prevents the Progression of Colorectal Cancer. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 133 32042551
2007 The dual role of p55 tumour necrosis factor-alpha receptor in Actinobacillus actinomycetemcomitans-induced experimental periodontitis: host protection and tissue destruction. Clinical and experimental immunology 109 17177972
2001 Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis. Antimicrobial agents and chemotherapy 106 11181364
1998 A p55 TNF receptor immunoadhesin prevents T cell-mediated intestinal injury by inhibiting matrix metalloproteinase production. Journal of immunology (Baltimore, Md. : 1950) 105 9558121
1990 Internuclear exchange of an inner nuclear membrane protein (p55) in heterokaryons: in vivo evidence for the interaction of p55 with the nuclear lamina. The Journal of cell biology 101 2277058
2018 Promoter Methylation-Regulated miR-145-5p Inhibits Laryngeal Squamous Cell Carcinoma Progression by Targeting FSCN1. Molecular therapy : the journal of the American Society of Gene Therapy 100 30341010
2004 The p55 TNF receptor mediates TNF inhibition of osteoblast differentiation independently of apoptosis. American journal of physiology. Endocrinology and metabolism 98 15625085
2013 MicroRNA-133a regulates the mRNAs of two invadopodia-related proteins, FSCN1 and MMP14, in esophageal cancer. British journal of cancer 95 24196787
2000 The p55 TNF-alpha receptor plays a critical role in T cell alloreactivity. Journal of immunology (Baltimore, Md. : 1950) 95 10623807
1995 Tumor necrosis factor (TNF)-alpha activates c-raf-1 kinase via the p55 TNF receptor engaging neutral sphingomyelinase. The EMBO journal 88 7720706
2000 Regulation of protein 4.1R, p55, and glycophorin C ternary complex in human erythrocyte membrane. The Journal of biological chemistry 84 10831591
1996 Identification of anisomycin-activated kinases p45 and p55 in murine cells as MAPKAP kinase-2. Oncogene 83 8632902
2017 Long non-coding RNA PCAT-1 contributes to tumorigenesis by regulating FSCN1 via miR-145-5p in prostate cancer. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 81 28922730
1999 Protein transport and flagellum assembly dynamics revealed by analysis of the paralysed trypanosome mutant snl-1. Journal of cell science 81 10523512
2001 Roles of tumor necrosis factor p55 and p75 receptors in TNF-alpha-induced vascular permeability. American journal of physiology. Cell physiology 78 11546653
2016 Arabidopsis seed germination speed is controlled by SNL histone deacetylase-binding factor-mediated regulation of AUX1. Nature communications 77 27834370
2004 Osteoporosis, mineral metabolism, and serum soluble tumor necrosis factor receptor p55 in viral cirrhosis. The Journal of clinical endocrinology and metabolism 72 15356028
1997 Ligand-induced formation of p55 and p75 tumor necrosis factor receptor heterocomplexes on intact cells. The Journal of biological chemistry 69 9099731
1999 TNF receptor p55 plays a pivotal role in murine keratinocyte apoptosis induced by ultraviolet B irradiation. Journal of immunology (Baltimore, Md. : 1950) 68 9973400
1997 Low-molecular-weight tumor necrosis factor receptor p55 controls induction of autoimmune heart disease. Circulation 64 9024154
2016 miR-429 functions as a tumor suppressor by targeting FSCN1 in gastric cancer cells. OncoTargets and therapy 60 27042104
2013 Tumor necrosis factor in traumatic brain injury: effects of genetic deletion of p55 or p75 receptor. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 60 23611870
2000 Human p55(CDC)/Cdc20 associates with cyclin A and is phosphorylated by the cyclin A-Cdk2 complex. Biochemical and biophysical research communications 60 10679238
1998 Distinct adapter proteins mediate acid versus neutral sphingomyelinase activation through the p55 receptor for tumor necrosis factor. Journal of leukocyte biology 60 9620659
1995 A protein related to a proteasomal subunit binds to the intracellular domain of the p55 TNF receptor upstream to its 'death domain'. FEBS letters 59 7601280
2001 Tumor necrosis factor-alpha and its receptors, p55 and p75, in gingiva of adult periodontitis. Journal of dental research 58 11499508
1999 Vif and the p55(Gag) polyprotein of human immunodeficiency virus type 1 are present in colocalizing membrane-free cytoplasmic complexes. Journal of virology 58 10074112
2006 Whirlin complexes with p55 at the stereocilia tip during hair cell development. Proceedings of the National Academy of Sciences of the United States of America 57 16829577
2002 Pathogenic roles of tumor necrosis factor receptor p55-mediated signals in dimethylnitrosamine-induced murine liver fibrosis. Laboratory investigation; a journal of technical methods and pathology 55 12003998
2008 Tumor necrosis factor p55 and p75 receptors are involved in chemical-induced apoptosis of dentate granule neurons. Journal of neurochemistry 53 18373618
1997 TNF receptor p55 controls early acute graft-versus-host disease. Journal of immunology (Baltimore, Md. : 1950) 52 9164935
2020 Knockdown of lncRNA CCAT1 enhances sensitivity of paclitaxel in prostate cancer via regulating miR-24-3p and FSCN1. Cancer biology & therapy 49 32089062
1997 Constitutive shedding of both p55 and p75 murine TNF receptors in vivo. Journal of immunology (Baltimore, Md. : 1950) 47 9103455
1996 Tumor necrosis factor-alpha induces activation of coagulation and fibrinolysis in baboons through an exclusive effect on the p55 receptor. Blood 47 8704250
2021 Exosomal lncRNA LINC01711 facilitates metastasis of esophageal squamous cell carcinoma via the miR-326/FSCN1 axis. Aging 46 34370713
2003 Interactions between p-33 and p-55 domains of the Helicobacter pylori vacuolating cytotoxin (VacA). The Journal of biological chemistry 46 14593124
2013 miR-133a functions as a tumor suppressor and directly targets FSCN1 in pancreatic cancer. Oncology research 45 25198665
1994 TNF-alpha, the great imitator: role of p55 and p75 TNF receptors in hematopoiesis. Stem cells (Dayton, Ohio) 44 7535144
2017 EGFR conjunct FSCN1 as a Novel Therapeutic Strategy in Triple-Negative Breast Cancer. Scientific reports 43 29142206
2009 Absence of p55 TNF receptor reduces atherosclerosis, but has no major effect on angiotensin II induced aneurysms in LDL receptor deficient mice. PloS one 43 19582157
2014 The role of microRNA-133b and its target gene FSCN1 in gastric cancer. Journal of experimental & clinical cancer research : CR 42 25433493
2017 FSCN1 gene polymorphisms: biomarkers for the development and progression of breast cancer. Scientific reports 41 29162880
2015 MiR-24 functions as a tumor suppressor in nasopharyngeal carcinoma through targeting FSCN1. Journal of experimental & clinical cancer research : CR 41 26503504
1994 Expression of TNF-alpha and TNFR p55 in cultured amniochorion. American journal of reproductive immunology (New York, N.Y. : 1989) 38 7880403
1998 Mice deficient in tumor necrosis factor receptors p55 and p75, interleukin-4, or inducible nitric oxide synthase are susceptible to endotoxin-induced uveitis. Investigative ophthalmology & visual science 37 9501881
1998 C-terminal tripeptide Ser-Asn-Leu (SNL) of human D-aspartate oxidase is a functional peroxisome-targeting signal. The Biochemical journal 37 9820813
1996 TNF receptor p55 plays a major role in centrally mediated increases of serum IL-6 and corticosterone after intracerebroventricular injection of TNF. Journal of immunology (Baltimore, Md. : 1950) 37 8955207
2020 SOX2 Promotes Brain Metastasis of Breast Cancer by Upregulating the Expression of FSCN1 and HBEGF. Molecular therapy oncolytics 36 32322668
2018 LincRNA-ROR promotes metastasis and invasion of esophageal squamous cell carcinoma by regulating miR-145/FSCN1. OncoTargets and therapy 36 29430188
2016 miR-200b inhibits migration and invasion in non-small cell lung cancer cells via targeting FSCN1. Molecular medicine reports 36 27356635
1993 The release of soluble p55 TNF receptor from U937 cells studied by a new p55 immunoassay. Journal of immunological methods 36 8394856
2017 20(S)-Rg3 blocked epithelial-mesenchymal transition through DNMT3A/miR-145/FSCN1 in ovarian cancer. Oncotarget 35 28881818
2011 Role of P27 -P55 operon from Mycobacterium tuberculosis in the resistance to toxic compounds. BMC infectious diseases 34 21762531
2010 Electroacupuncture versus celecoxib for neuropathic pain in rat SNL model. Neuroscience 34 20654703
2009 Erythrocyte scaffolding protein p55/MPP1 functions as an essential regulator of neutrophil polarity. Proceedings of the National Academy of Sciences of the United States of America 33 19897731
2003 Upregulation of p55 and p75 receptors mediating TNF-alpha transport across the injured blood-spinal cord barrier. Journal of molecular neuroscience : MN 33 14593216
2022 FSCN1 acts as a promising therapeutic target in the blockade of tumor cell motility: a review of its function, mechanism, and clinical significance. Journal of Cancer 32 35711849
2023 Electroacupuncture alleviates neuropathic pain caused by SNL by promoting M2 microglia polarization through PD-L1. International immunopharmacology 31 37573685
2015 Reverse Correlation between MicroRNA-145 and FSCN1 Affecting Gastric Cancer Migration and Invasion. PloS one 31 26010149
2015 MicroRNA-145 inhibits migration and invasion by down-regulating FSCN1 in lung cancer. International journal of clinical and experimental medicine 31 26309531
2015 The role of FSCN1 in migration and invasion of pituitary adenomas. Molecular and cellular endocrinology 31 26522130
1998 Function of p55 and its nonerythroid homologues. Current opinion in hematology 31 9570704
1995 Genomic structure and nucleotide sequence of the p55 gene of the puffer fish Fugu rubripes. Genomics 31 7558025
1991 Synergistic action of monoclonal antibodies directed at p55 and p75 chains of the human IL-2-receptor. Journal of immunology (Baltimore, Md. : 1950) 31 1988501
2000 Activation of ERK1/2 and cPLA(2) by the p55 TNF receptor occurs independently of FAN. Biochemical and biophysical research communications 30 10913368
2015 MicroRNA-145 and MicroRNA-133a Inhibited Proliferation, Migration, and Invasion, While Promoted Apoptosis in Hepatocellular Carcinoma Cells Via Targeting FSCN1. Digestive diseases and sciences 29 26173501
2021 Electroacupuncture improves neuronal plasticity through the A2AR/cAMP/PKA signaling pathway in SNL rats. Neurochemistry international 27 33577869
2021 LYAR Promotes Colorectal Cancer Progression by Upregulating FSCN1 Expression and Fatty Acid Metabolism. Oxidative medicine and cellular longevity 27 35069968
2019 miR-145 Contributes to the Progression of Cervical Carcinoma by Directly Regulating FSCN1. Cell transplantation 27 31271056
2012 Palmitoylation of MPP1 (membrane-palmitoylated protein 1)/p55 is crucial for lateral membrane organization in erythroid cells. The Journal of biological chemistry 27 22496366
2002 Tumor necrosis factor receptor p55 and p75 deficiency protects mice from developing experimental autoimmune myasthenia gravis. Journal of neuroimmunology 27 11777546
2019 MicroRNA-145 regulates immune cytokines via targeting FSCN1 in Staphylococcus aureus-induced mastitis in dairy cows. Reproduction in domestic animals = Zuchthygiene 26 30974481
2011 Chronic inflammation and pain in a tumor necrosis factor receptor (TNFR) (p55/p75-/-) dual deficient murine model. Translational research : the journal of laboratory and clinical medicine 26 22687964
2023 Ginsenoside Rb2 inhibits p300-mediated SF3A2 acetylation at lysine 10 to promote Fscn1 alternative splicing against myocardial ischemic/reperfusion injury. Journal of advanced research 25 38101749
2022 LncRNA CRNDE promotes cell proliferation, migration and invasion of ovarian cancer via miR-423-5p/FSCN1 axis. Molecular and cellular biochemistry 25 35166986
2001 Phosphorylation of the tumor necrosis factor receptor CD120a (p55) recruits Bcl-2 and protects against apoptosis. The Journal of biological chemistry 25 11278725
2013 The role of MPP1/p55 and its palmitoylation in resting state raft organization in HEL cells. Biochimica et biophysica acta 24 23507198
2020 MicroRNA-488 inhibits proliferation and motility of tumor cells via downregulating FSCN1, modulated by Notch3 in breast carcinomas. Cell death & disease 23 33099573
2015 The clinical significance of FSCN1 in non-small cell lung cancer. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 23 26211585
2021 GRHL3 activates FSCN1 to relax cell-cell adhesions between migrating keratinocytes during wound reepithelialization. JCI insight 22 34494554
2021 ADORA2A-AS1 Restricts Hepatocellular Carcinoma Progression via Binding HuR and Repressing FSCN1/AKT Axis. Frontiers in oncology 22 34733789
2010 Lack of TNFR p55 results in heightened expression of IFN-γ and IL-17 during the development of reactive arthritis. Journal of immunology (Baltimore, Md. : 1950) 22 20810989
2001 Cloning and characterization of the human retina-specific gene MPP4, a novel member of the p55 subfamily of MAGUK proteins. Genomics 22 11414766
2001 Tumor necrosis factor soluble receptor p55 and lipid peroxidation in patients with acute alcoholic hepatitis. The American journal of gastroenterology 22 11774950
1996 Complete genomic organization of the human erythroid p55 gene (MPP1), a membrane-associated guanylate kinase homologue. Genomics 22 8824805
1990 Expression of the human IL-2 receptor on lymphocytes involves rapid turnover of its p55 alpha-subunit (Tac). Journal of immunology (Baltimore, Md. : 1950) 21 2212668
2012 The biological function of the WD40 repeat-containing protein p55/Caf1 in Drosophila. Developmental dynamics : an official publication of the American Association of Anatomists 20 22241697
2021 Preventing alloimmune rejection using circular RNA FSCN1-silenced dendritic cells in heart transplantation. The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation 19 34052126
2017 XIAP 3'-untranslated region as a ceRNA promotes FSCN1 function in inducing the progression of breast cancer by binding endogenous miR-29a-5p. Oncotarget 19 28186968