Affinage

FABP3

Fatty acid-binding protein, heart · UniProt P05413

Length
133 aa
Mass
14.9 kDa
Annotated
2026-06-09
100 papers in source corpus 25 papers cited in narrative 25 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

FABP3 (heart-type FABP/MDGI) is an intracellular lipid carrier that binds long-chain fatty acids within a beta-barrel cavity and delivers them to drive lipid metabolism and lipid-dependent signaling across heart, muscle, neurons, and vasculature (PMID:3230093, PMID:27006775). Direct ligand binding has been established by saturable [3H]palmitate binding (PMID:3230093), high-resolution joint X-ray/neutron crystallography of an oleic-acid complex (PMID:27006775), and biophysical studies resolving two binding states with a thermodynamic preference for partial ligand release at physiological temperature (PMID:38777142); FABP3 also functions as a lysophosphatidic acid carrier that shuttles LPA to the nucleus to activate PPARγ (PMID:25426414). Genetically, FABP3 is required for efficient cytosolic fatty-acid shuttling and oxidation in skeletal muscle (PMID:12900378), and in the heart it physically interacts with PPARα to stabilize it and sustain fatty-acid-oxidation gene programs, loss of which produces glycolytic shift, lipotoxicity, and hypertrophy rescued by fenofibrate (PMID:34458345). Beyond canonical metabolic transport, FABP3 binds integrin α-subunit cytoplasmic tails to suppress integrin activation, adhesion, and invasion (PMID:20802519), serves as a selective autophagy substrate of OPTN that controls mesenchymal stem cell fate and bone mass (PMID:33143524), and remodels membrane phospholipid composition to reduce fluidity and trigger PERK–eIF2α ER stress in aged muscle (PMID:33168829). In dopaminergic neurons FABP3 directly interacts with α-synuclein and, in a fatty-acid-binding-dependent manner, promotes arachidonic-acid-induced αSyn oligomerization, with Fabp3 deletion conferring resistance to MPTP neurodegeneration and blocking αSyn fibril propagation (PMID:24855640, PMID:33636166). FABP3 also controls Gad67 promoter DNA methylation in cortical GABAergic interneurons via MeCP2/HDAC1 recruitment (PMID:30341178) and drives ROS-dependent injury pathways including endothelial ferroptosis and ischemia-reperfusion neuronal apoptosis (PMID:38218337, PMID:39008165).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1988 High

    Established the foundational identity of MDGI/FABP3 as a saturable fatty-acid-binding protein that can facilitate lipid uptake by cells, answering whether it is a bona fide lipid carrier.

    Evidence Radioligand saturation binding with [3H]palmitate plus cell uptake comparison

    PMID:3230093

    Open questions at the time
    • Did not resolve the structural basis of binding
    • No physiological tissue context for the transport function
  2. 1989 Medium

    Raised the possibility of a nuclear/chromatin pool of FABP3 beyond its cytosolic role, hinting at a gene-regulatory function.

    Evidence Subcellular fractionation, chromatin purification, and immunogold EM in mammary epithelium

    PMID:2918043

    Open questions at the time
    • Nuclear antigen detected as a 70 kDa species whose relationship to 13 kDa FABP3 was unresolved
    • No molecular mechanism for chromatin association
  3. 2003 High

    Defined the in vivo metabolic requirement for FABP3 by showing it is needed for efficient cytosolic fatty-acid shuttling and oxidation but is not absolutely essential.

    Evidence H-FABP knockout mouse with isolated soleus muscle FA transport, oxidation, and esterification assays

    PMID:12900378

    Open questions at the time
    • Residual FA metabolism implies compensating carriers not identified
    • Does not address signaling or nuclear functions
  4. 2010 High

    Revealed a non-metabolic function: FABP3 binds integrin α cytoplasmic tails to suppress integrin activation and tumor cell invasion.

    Evidence Integrin tail peptide pulldown, Co-IP, integrin activity, adhesion and invasion assays in breast cancer lines

    PMID:20802519

    Open questions at the time
    • Whether lipid binding is required for integrin regulation untested
    • In vivo tumor relevance limited to cell lines and tissue arrays
  5. 2014 High

    Connected FABP3 lipid binding to neurodegeneration by showing it interacts with α-synuclein and promotes fatty-acid-dependent αSyn oligomerization.

    Evidence Co-IP, FA-binding-deficient mutant, PC12 oligomerization assay, and Fabp3-/- MPTP mouse model

    PMID:24855640

    Open questions at the time
    • Stoichiometry and structural basis of FABP3/αSyn interaction unknown
    • Whether arachidonic acid is delivered by FABP3 directly not shown structurally
  6. 2014 High

    Identified FABP3 as a lysophosphatidic acid carrier that delivers LPA to the nucleus to activate PPARγ, broadening its ligand repertoire beyond fatty acids.

    Evidence LPA-bead affinity pulldown, siRNA knockdown, PPARγ reporter, and nuclear LPA fractionation in coronary endothelial cells

    PMID:25426414

    Open questions at the time
    • Mechanism of nuclear import of the FABP3-LPA complex not defined
    • Direct physical FABP3-PPARγ contact not demonstrated
  7. 2016 High

    Provided atomic-resolution structural definition of the FABP3 fatty-acid binding pocket, including hydrogen positions and an internal water cluster.

    Evidence Joint X-ray (0.98 Å) and neutron (1.90 Å) crystallography of the oleic-acid complex with QTAIM analysis

    PMID:27006775

    Open questions at the time
    • Static structure does not capture binding/release dynamics
    • Does not address protein-protein interaction surfaces
  8. 2018 High

    Uncovered an epigenetic role for FABP3 in controlling Gad67 promoter DNA methylation in cortical GABAergic interneurons.

    Evidence Fabp3 KO mice with ChIP for MeCP2/HDAC1, bisulfite methylation analysis, and methionine behavioral rescue

    PMID:30341178

    Open questions at the time
    • Molecular link between cytosolic lipid binding and methyl-donor metabolism not defined
    • Whether nuclear FABP3 acts directly at the promoter unresolved
  9. 2020 High

    Showed FABP3 is a selective autophagy substrate of OPTN whose accumulation reprograms mesenchymal stem cell fate toward adipogenesis and bone loss.

    Evidence Optn-/- and aged mouse models with MSC transplantation, lentiviral Optn re-expression, and in vivo FABP3 knockdown rescue

    PMID:33143524

    Open questions at the time
    • Degron/recognition motif for OPTN targeting not mapped
    • Downstream lipid effector driving fate switch not identified
  10. 2020 High

    Demonstrated that FABP3 remodels membrane phospholipid composition to reduce fluidity and induce PERK-eIF2α ER stress, impairing protein synthesis in aged muscle.

    Evidence Gain- and loss-of-function in mouse skeletal muscle with lipidomics, membrane fluidity, ER stress pathway analysis, and recovery model

    PMID:33168829

    Open questions at the time
    • How a soluble FA carrier alters bulk phospholipid acyl composition mechanistically unclear
    • Direct link between FABP3 and ER stress sensor activation not biochemically defined
  11. 2021 High

    Established a direct FABP3-PPARα axis in which FABP3 stabilizes PPARα to sustain cardiac fatty-acid-oxidation gene programs and prevent hypertrophy.

    Evidence Fabp3 KO TAC mouse, Co-IP, multi-omics, and fenofibrate rescue

    PMID:34458345

    Open questions at the time
    • Mechanism by which FABP3 prevents PPARα degradation not defined
    • Whether ligand loading modulates the interaction untested
  12. 2021 High

    Extended the α-synuclein link in vivo by showing FABP3 deletion suppresses αSyn fibril propagation and protects dopaminergic neurons.

    Evidence Fabp3 KO mice with intrastriatal labeled monomeric/fibrillar αSyn tracking, phospho-αSyn staining, TH neuron counts, and motor tests

    PMID:33636166

    Open questions at the time
    • Cell-type-specific contribution of FABP3 to spreading not dissected
    • Whether intervention after seeding is effective untested
  13. 2022 Medium

    Identified a FABP3-CACT interaction that channels fatty-acid oxidation to drive fibroblast proliferation and vascular fibrosis.

    Evidence Co-IP, gain/loss-of-function in adventitial fibroblasts, lipidomics, and etomoxir rescue

    PMID:34718429

    Open questions at the time
    • Single-lab Co-IP without reciprocal structural validation
    • Whether FABP3 delivers substrate to CACT directly not shown
  14. 2024 Medium

    Linked FABP3 to oxidative cell-death pathways, showing it mediates oxidized-phospholipid-induced endothelial ferroptosis via lipid peroxidation and GPX4 suppression.

    Evidence siRNA knockdown in HUVECs, CD36 knockdown, lipid peroxidation/iron assays, GPX4 blot, and ferrostatin-1 and vasodilation rescue

    PMID:38218337

    Open questions at the time
    • Direct molecular trigger linking FABP3 to GPX4 loss unknown
    • In vitro/ex vivo only
  15. 2024 Medium

    Showed FABP3 drives ROS-dependent mitophagy causing mitochondrial dysfunction and neuronal apoptosis in cerebral ischemia-reperfusion injury.

    Evidence MCAO mouse and OGD/R neuron models with lentiviral silencing, ROS measurement, and ATG5 and NAC rescue

    PMID:39008165

    Open questions at the time
    • How a lipid carrier generates ROS not defined
    • Single lab, mechanism upstream of ROS unclear
  16. 2024 Medium

    Resolved the dynamics of FABP3 ligand binding, revealing two binding states with a thermodynamic preference for partial release at body temperature.

    Evidence CW-EPR with spin-labeled stearic acid plus microscale thermophoresis

    PMID:38777142

    Open questions at the time
    • No mutagenesis to assign residues to each binding state
    • Functional consequence of two-state binding in cells untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how FABP3's single beta-barrel lipid-binding fold mechanistically gives rise to such divergent outputs—integrin regulation, nuclear receptor stabilization, membrane remodeling, epigenetic methylation control, and ROS-linked cell death—and whether ligand identity selects among these fates.
  • No unified model linking ligand loading state to choice of effector pathway
  • Nuclear targeting mechanism of FABP3 unresolved
  • Structural basis of protein-protein interactions (integrin, PPARα, αSyn, CACT) undetermined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008289 lipid binding 4 GO:0098772 molecular function regulator activity 2 GO:0140104 molecular carrier activity 2
Localization
GO:0005634 nucleus 2 GO:0005829 cytosol 2 GO:0005886 plasma membrane 1
Pathway
R-HSA-1430728 Metabolism 2 R-HSA-162582 Signal Transduction 2 R-HSA-5357801 Programmed Cell Death 2 R-HSA-9612973 Autophagy 2
Partners
CACTCAV1ITGAOPTNPPARAPPARGSNCA

Evidence

Reading pass · 25 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1988 MDGI (FABP3) was demonstrated to bind [3H]palmitic acid in a saturable manner and to be complexed with endogenous free fatty acids, establishing it as a fatty acid-binding/lipid carrier protein. Bound palmitic acid was more rapidly taken up by target cells than free palmitic acid, suggesting a lipid carrier function. Radioligand binding assay ([3H]palmitic acid saturation binding), radioimmunoassay, cell uptake assay Journal of cellular biochemistry High 3230093
1989 MDGI (FABP3) protein localizes to both the cytosol/microsomal fraction and, via a cross-reactive 70 kDa antigen, to the chromatin of nuclei in differentiated mammary epithelial cells. Nuclear association was confirmed by salt extraction, DNase I digestion, chromatin purification, and immunogold electron microscopy. Western blotting of subcellular fractions, immunogold electron microscopy, chromatin purification, DNase I digestion Journal of cellular physiology Medium 2918043
1991 MDGI (FABP3) inhibits L(+)-lactate-, arachidonic acid-, and 15-S-HETE-induced supersensitivity of neonatal rat heart cells to beta-adrenergic stimulation (specifically a beta2-receptor subpopulation). A synthetic C-terminal peptide (residues 121–131) mimics this effect. Direct lipid binding of arachidonic acid or beta-adrenergic agonists to MDGI was excluded as the mechanism; instead, MDGI appears to interfere with beta2-adrenergic receptor function. Neonatal rat cardiomyocyte supersensitivity assay, synthetic peptide functional testing, lipid-binding exclusion assay, radioligand receptor binding (CGP 12177) Molecular and cellular biochemistry Medium 1646956
2003 H-FABP null mutation in mice reduces skeletal muscle fatty acid transport by ~30%, decreases palmitate oxidation by ~71%, and reduces TAG esterification at rest, despite normal sarcolemmal FABP and CD36 content, demonstrating that H-FABP is required for efficient cytosolic FA shuttling in skeletal muscle but is not absolutely essential (residual FA metabolism persists). H-FABP knockout mouse model, isolated soleus muscle metabolism assays (FA transport, palmitate oxidation, TAG esterification, glycogen utilization), enzyme activity assays, immunoblotting American journal of physiology. Endocrinology and metabolism High 12900378
2004 In alveolar type II cells from E/H-FABP double-knockout mice, palmitic acid uptake, beta-oxidation, and incorporation into neutral lipids and total phosphatidylcholine are reduced, while DPPC synthesis shifts from de novo to reacylation. Caveolin-1 and PPARγ expression increase and caveolin-1 interacts with PPARγ. PPARγ agonist pioglitazone restores wild-type-like fatty acid transport and utilization, indicating H-FABP (together with E-FABP) mediates DPPC synthesis and PPARγ-linked fatty acid signaling in lung TII cells. Double-knockout mouse model, radiolabeled palmitic acid transport/utilization assays, Western blot, RT-PCR, co-immunoprecipitation (caveolin-1/PPARγ), dietary PPARγ agonist rescue Biochimica et biophysica acta Medium 15164767
2010 MDGI (FABP3) binds directly to the cytoplasmic tails of integrin α-subunits and its expression suppresses integrin activity (active conformation), reducing integrin adhesion to type I collagen and fibronectin and inhibiting cell migration and invasion in breast cancer cell lines. Direct binding assay (pulldown with integrin α cytoplasmic tail peptides), Co-IP, integrin activity assays (active-conformation antibody), adhesion assays, invasion assays, tissue microarray Oncogene High 20802519
2012 FABP3 is a direct target of muscle-specific miR-1; overexpression of miR-1 in cardiomyocytes decreases FABP3 protein levels. An inverse relationship between myocardial miR-1 expression and circulating FABP3 levels was confirmed in mouse models (pressure overload, fasting) and human patients with cardiac hypertrophy, establishing FABP3 as a miR-1-regulated secreted protein. Proteomic analysis of CM secretome (MudPIT), transgenic miR-1 overexpression mouse model, Western blot, ELISA, qRT-PCR, luciferase reporter (implied by targeting) Journal of the American College of Cardiology Medium 23141496
2014 FABP3 protein directly interacts with α-synuclein in the substantia nigra pars compacta. FABP3 overexpression aggravates arachidonic acid-induced α-synuclein oligomerization and promotes cell death in PC12 cells, whereas a FABP3 mutant lacking fatty-acid binding capacity does not, demonstrating that FA binding by FABP3 is required for αSyn oligomerization. Fabp3−/− mice are resistant to MPTP-induced dopaminergic neurodegeneration. Co-immunoprecipitation (FABP3/αSyn interaction), FABP3 knockout mouse (MPTP model), overexpression of wild-type vs. FA-binding-deficient FABP3 mutant in PC12 cells, cell viability assay, αSyn oligomerization assay The Journal of biological chemistry High 24855640
2014 FABP3 upregulates expression of SREBP1 and PPARγ to increase lipid droplet accumulation in dairy cow mammary epithelial cells. Oleic acid, stearic acid, and palmitic acid increase lipid droplet accumulation partly by affecting FABP3 expression, positioning FABP3 upstream of SREBP1/PPARγ in the milk fat synthesis signaling pathway. FABP3 overexpression and siRNA knockdown in bovine mammary epithelial cells, qRT-PCR, Western blotting, fluorescent immunostaining of lipid droplets In vitro cellular & developmental biology. Animal Medium 24947174
2014 FABP3 functions as a lysophosphatidic acid (LPA) carrier protein in human coronary artery endothelial cells, shuttling LPA to the nucleus to activate PPARγ. LPA-coated agarose bead pulldown identified FABP3 as an LPA-binding protein; FABP3 siRNA knockdown abolished LPA-induced PPARγ activation and reduced nuclear LPA accumulation. LPA-coated agarose bead pulldown/affinity chromatography, siRNA knockdown, PPARγ luciferase reporter assay, nuclear fractionation with LPA quantification FEBS open bio High 25426414
2014 A FABP3 frameshift variant (E132fs) forms cellular aggregates and is unstable when expressed in cultured cells, unlike missense variants that retain normal intracellular localization, indicating the frameshift disrupts normal FABP3 protein folding/stability. Expression of patient-derived FABP3 variants in cultured cells, immunofluorescence for localization, stability assay Human molecular genetics Medium 25027319
2015 E2F8 transcription factor promotes FABP3 expression, and FABP3 in turn promotes hepatic steatosis. E2f8 morpholino knockdown suppressed fabp3 expression and ameliorated hepatic steatosis in diet-induced obese zebrafish; E2F8 overexpression in HepG2 cells increased FABP3 expression, establishing an E2F8→FABP3 regulatory axis in hepatic lipid accumulation. Morpholino antisense knockdown in zebrafish, transcriptome/proteome analysis, E2F8 overexpression in HepG2 cells Nutrition & metabolism Medium 26052340
2016 FABP3 is expressed in high-resolution X-ray/neutron crystal structure in complex with oleic acid (0.98 Å X-ray / 1.90 Å neutron resolution). The structure revealed a large internal water cluster, precise positions of hydrogen/deuterium atoms, FA binding pocket electrostatics, and H···H contacts between FA and conserved hydrophobic residues stabilizing long-chain FAs. High-resolution X-ray crystallography (0.98 Å) and neutron protein crystallography (1.90 Å), joint X-ray/neutron structure refinement, Bader QTAIM analysis IUCrJ High 27006775
2018 FABP3 in the anterior cingulate cortex (ACC) is expressed exclusively in GABAergic interneurons and controls DNA methylation of the Gad67 promoter. Fabp3 KO mice show hypomethylation of the Gad67 promoter, decreased binding of MeCP2 and HDAC1 to the promoter, and upregulated Gad67 mRNA in ACC. Methionine supplementation restores Gad67 promoter methylation and normalizes behavior in Fabp3 KO mice. Fabp3 knockout mice, in situ hybridization, ChIP (MeCP2, HDAC1 binding to Gad67 promoter), bisulfite methylation analysis, methionine rescue experiment, behavioral testing The Journal of neuroscience High 30341178
2018 FABP3 ligands that bind to the fatty acid binding domain of FABP3 (identified by ANS displacement assay with recombinant FABP3) significantly reduce arachidonic acid-induced α-synuclein oligomerization in neuro-2A cells co-overexpressing FABP3 and αSyn, confirming that blocking FA binding to FABP3 prevents FABP3-mediated αSyn aggregation. ANS fluorescence displacement assay with recombinant FABP3, αSyn oligomerization assay in neuro-2A cells, FABP3/αSyn co-overexpression Brain research Medium 30496735
2020 OPTN (optineurin) acts as a selective autophagy receptor that targets FABP3 for degradation. FABP3 was identified as a novel selective autophagy substrate of OPTN. FABP3 accumulation (due to reduced OPTN in aging) promotes adipogenesis and inhibits osteogenesis of mesenchymal stem cells, leading to bone loss. FABP3 knockdown in optn−/− mice and aged mice rescues bone loss. Optn−/− mouse model, aged mouse model, MSC transplantation rescue, lentiviral Optn re-expression, FABP3 knockdown in vivo, autophagy substrate identification assays, micro-CT, histomorphometry Autophagy High 33143524
2020 FABP3 overexpression in skeletal muscle remodels membrane lipid composition by decreasing polyunsaturated phospholipid acyl chains and increasing sphingomyelin and lysophosphatidylcholine, reducing membrane fluidity and inducing ER stress via the PERK-eIF2α pathway, thereby inhibiting protein synthesis. FABP3 knockdown in aged muscles restores a young-like lipid composition, reduces ER stress, and improves protein synthesis and muscle recovery. FABP3 overexpression and knockdown in mouse skeletal muscle, lipidomics, membrane fluidity assay, ER stress pathway analysis (Western blot for PERK, eIF2α), immobilization/recovery model, protein synthesis assay Nature communications High 33168829
2021 FABP3 directly interacts with PPARα, preventing PPARα degradation and synergistically modulating its transcriptional activity on Mlycd and Gck. In Fabp3-deficient hearts under hypertrophic stimulation, this interaction is lost, resulting in increased glycolysis, toxic lipid accumulation, and reduced fatty acid oxidation and ATP production. PPARα agonist fenofibrate rescues the pro-hypertrophic effects of Fabp3 deficiency. Fabp3 knockout mouse (TAC model), multi-omics (metabolomics, transcriptomics), Co-IP (FABP3/PPARα interaction), fenofibrate rescue experiment, echocardiography Frontiers in cardiovascular medicine High 34458345
2021 FABP3 deletion in mice suppresses α-synuclein fibril spread from striatum to substantia nigra pars compacta. In Fabp3−/− mice, accumulation of both monomeric and fibrillar exogenous αSyn in SNpc was drastically decreased, αSyn fibril-induced seeding of endogenous αSyn into phosphorylated/filamentous aggregates was prevented, and dopaminergic neuron loss and motor impairment were attenuated. Fabp3 knockout mice, intrastriatal injection of ATTO550-labeled monomeric and fibrillar αSyn, immunofluorescence tracking, phospho-αSyn immunostaining, TH-positive neuron counting, behavioral motor tests Brain research High 33636166
2022 FABP3 binds to carnitine/acylcarnitine carrier protein (CACT), a key enzyme in fatty acid oxidation (FAO), in aorta adventitial fibroblasts. FABP3 upregulation promotes CACT and CPT1A expression, increases ATP levels, and drives enhanced FAO, AAF proliferation, and extracellular matrix production, contributing to vascular fibrosis in Takayasu's arteritis. Co-immunoprecipitation (FABP3/CACT), immunohistochemistry, FABP3 knockdown and overexpression in fibroblasts, lipidomics, RT-qPCR, Western blot, FAO inhibitor (etomoxir) rescue, ELISA Rheumatology (Oxford, England) Medium 34718429
2024 PGPC (an oxidized phospholipid) increases FABP3 expression via CD36 receptor signaling in endothelial cells. FABP3 mediates PGPC-induced ferroptosis by promoting lipid peroxidation and iron accumulation while suppressing GPX4 expression. FABP3 silencing reverses PGPC-induced ferroptosis and impaired endothelium-dependent vasodilation; FABP3 inhibitors block PGPC-induced vascular dysfunction. FABP3 siRNA knockdown in HUVECs, CD36 siRNA knockdown, lipid peroxidation assay, ferrous iron measurement, GPX4 western blot, mitochondrial membrane potential, aortic vasodilation assay, ferrostatin-1 rescue Journal of lipid research Medium 38218337
2024 FABP3 activates mitochondrial autophagy through ROS generation, leading to mitochondrial dysfunction and neuronal apoptosis in cerebral ischemia-reperfusion injury. FABP3 silencing in vivo reduces brain infarct volume, neuronal apoptosis, and inflammation; in vitro, FABP3 silencing restores cell viability and reduces mitochondrial ROS. NAC (free radical scavenger) blocks FABP3-induced mitophagy, and sh-ATG5 confirms that mitophagy is required for FABP3-induced mitochondrial dysfunction. MCAO mouse model, lentiviral FABP3 silencing, OGD/R neuronal model, ROS measurement, flow cytometry (apoptosis), TEM (autophagosome), JC-1 (mitochondrial membrane potential), LC3 Western blot, immunofluorescence colocalization, ATG5 knockdown rescue, NAC rescue Neurotoxicity research Medium 39008165
2024 Fatty acid binding to FABP3 occurs in two distinct binding states ('intermediately' and 'strongly' bound) as revealed by CW-EPR spectroscopy with spin-labeled stearic acid. The proportion of bound ligand depends on FABP3 concentration and temperature, with the more dynamic 'intermediately bound' state predominating at body temperature, indicating thermodynamic preference for partial release at physiological conditions. CW electron paramagnetic resonance (EPR) spectroscopy with 5/16-DOXYL stearic acid, microscale thermophoresis (MST), dynamic light scattering, bioinformatic modeling The Journal of biological chemistry Medium 38777142
2016 miR-192-5p targets FABP3 mRNA in H9c2 cardiomyocytes; overexpression of miR-192-5p reduces FABP3 levels and augments hypoxia/reoxygenation-induced apoptosis, while restoration of FABP3 prevents apoptosis in miR-192-5p-overexpressing cells, demonstrating that FABP3 acts downstream of miR-192-5p as a pro-survival factor during cardiac ischemia/reperfusion. miR-192-5p overexpression and inhibition in H9c2 cells, FABP3 restoration (overexpression), H/R model, Annexin V apoptosis assay, Bax/Bcl-2 ratio, luciferase reporter (FABP3 as miR-192-5p target) Journal of biochemical and molecular toxicology Medium 27780314
2020 The glioblastoma homing peptide CooP binds to FABP3 (MDGI) with micromolar affinity (KD ~2.18 µM). Alanine scanning identified glycine residues at positions 5 and 7 of CooP as critical for FABP3 binding; their replacement abolished in vitro cell binding and in vivo glioblastoma homing. Microscale thermophoresis (MST), surface plasmon resonance (SPR), alanine scan mutagenesis of peptide, in vitro cell binding, in vivo intracranial glioblastoma xenograft homing Cancers Medium 32650473

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2008 ACSL1, AGPAT6, FABP3, LPIN1, and SLC27A6 are the most abundant isoforms in bovine mammary tissue and their expression is affected by stage of lactation. The Journal of nutrition 183 18492828
2000 Human heart-type cytoplasmic fatty acid-binding protein (H-FABP) for the diagnosis of acute myocardial infarction. Clinical evaluation of H-FABP in comparison with myoglobin and creatine kinase isoenzyme MB. Clinical chemistry and laboratory medicine 141 10905760
2020 Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3. Autophagy 127 33143524
2020 FABP3-mediated membrane lipid saturation alters fluidity and induces ER stress in skeletal muscle with aging. Nature communications 115 33168829
2014 Functional characterization of FABP3, 5 and 7 gene variants identified in schizophrenia and autism spectrum disorder and mouse behavioral studies. Human molecular genetics 87 25027319
2020 Heart-Type Fatty Acid-Binding Protein (H-FABP) and its Role as a Biomarker in Heart Failure: What Do We Know So Far? Journal of clinical medicine 71 31936148
2014 FABP3 protein promotes α-synuclein oligomerization associated with 1-methyl-1,2,3,6-tetrahydropiridine-induced neurotoxicity. The Journal of biological chemistry 71 24855640
2009 A combined CXCL10, CXCL8 and H-FABP panel for the staging of human African trypanosomiasis patients. PLoS neglected tropical diseases 57 19554086
2012 The circulating level of FABP3 is an indirect biomarker of microRNA-1. Journal of the American College of Cardiology 55 23141496
2013 Fatty acid binding protein 3 (fabp3) is associated with insulin, lipids and cardiovascular phenotypes of the metabolic syndrome through epigenetic modifications in a Northern European family population. BMC medical genomics 54 23510163
2010 Association of genetic variants for FABP3 gene with back fat thickness and intramuscular fat content in pig. Molecular biology reports 53 20848210
2010 Comparison of the new high sensitive cardiac troponin T with myoglobin, h-FABP and cTnT for early identification of myocardial necrosis in the acute coronary syndrome. Clinical research in cardiology : official journal of the German Cardiac Society 53 20852870
1996 Silencing of the mammary-derived growth inhibitor (MDGI) gene in breast neoplasms is associated with epigenetic changes. Cancer research 53 8895735
2014 Functional analysis of FABP3 in the milk fat synthesis signaling pathway of dairy cow mammary epithelial cells. In vitro cellular & developmental biology. Animal 52 24947174
2012 Overexpression of FABP3 promotes apoptosis through inducing mitochondrial impairment in embryonic cancer cells. Journal of cellular biochemistry 52 22753283
2015 E2F8 promotes hepatic steatosis through FABP3 expression in diet-induced obesity in zebrafish. Nutrition & metabolism 45 26052340
2010 Investigation of porcine FABP3 and LEPR gene polymorphisms and mRNA expression for variation in intramuscular fat content. Molecular biology reports 44 20300864
2013 Association of polymorphisms in solute carrier family 27, isoform A6 (SLC27A6) and fatty acid-binding protein-3 and fatty acid-binding protein-4 (FABP3 and FABP4) with fatty acid composition of bovine milk. Journal of dairy science 42 23831098
2011 Circulating AST, H-FABP, and NGAL are early and accurate biomarkers of graft injury and dysfunction in a preclinical model of kidney transplantation. Annals of surgery 42 21997818
2008 Risk stratification of chronic heart failure patients by multiple biomarkers: implications of BNP, H-FABP, and PTX3. Circulation journal : official journal of the Japanese Circulation Society 42 18832778
2021 FABP3 Deficiency Exacerbates Metabolic Derangement in Cardiac Hypertrophy and Heart Failure via PPARα Pathway. Frontiers in cardiovascular medicine 41 34458345
2018 Development of FABP3 ligands that inhibit arachidonic acid-induced α-synuclein oligomerization. Brain research 41 30496735
2008 Identification of genetic polymorphisms in FABP3 and FABP4 and putative association with back fat thickness in Korean native cattle. BMB reports 41 18304447
2008 Fabp3 as a biomarker of skeletal muscle toxicity in the rat: comparison with conventional biomarkers. Toxicological sciences : an official journal of the Society of Toxicology 41 18308699
2003 Structure, linkage mapping and expression of the heart-type fatty acid-binding protein gene (fabp3 ) from zebrafish (Danio rerio). European journal of biochemistry 41 12869198
2003 Heart type fatty acid binding protein (H-FABP) is decreased in brains of patients with Down syndrome and Alzheimer's disease. Journal of neural transmission. Supplementum 41 15068254
2011 Fabp3 inhibits proliferation and promotes apoptosis of embryonic myocardial cells. Cell biochemistry and biophysics 40 21293949
2010 Mammary-derived growth inhibitor (MDGI) interacts with integrin α-subunits and suppresses integrin activity and invasion. Oncogene 40 20802519
2006 Pre-hospital detection of acute myocardial infarction with ultra-rapid human fatty acid-binding protein (H-FABP) immunoassay. International journal of cardiology 40 17097752
2012 Critical review and meta-analysis on the combination of heart-type fatty acid binding protein (H-FABP) and troponin for early diagnosis of acute myocardial infarction. Clinical biochemistry 39 23099200
2003 A null mutation in H-FABP only partially inhibits skeletal muscle fatty acid metabolism. American journal of physiology. Endocrinology and metabolism 37 12900378
2017 H-FABP: A new biomarker to differentiate between CT-positive and CT-negative patients with mild traumatic brain injury. PloS one 36 28419114
2010 Associations of A-FABP and H-FABP markers with the content of intramuscular fat in Beijing-You chicken. Animal biotechnology 36 20024783
2015 Heart-type fatty acid-binding protein (H-FABP) as an early diagnostic biomarker in patients with acute chest pain. Indian heart journal 35 26702681
2012 Urinary NT-proBNP, NGAL, and H-FABP may predict hemodynamic relevance of patent ductus arteriosus in very low birth weight infants. Neonatology 35 22222353
1989 A mammary-derived growth inhibitor (MDGI) related 70 kDa antigen identified in nuclei of mammary epithelial cells. Journal of cellular physiology 35 2918043
2008 The evolutionary relationship between the duplicated copies of the zebrafish fabp11 gene and the tetrapod FABP4, FABP5, FABP8 and FABP9 genes. The FEBS journal 34 18445037
2004 Pericardial fluid level of heart-type cytoplasmic fatty acid-binding protein (H-FABP) is an indicator of severe myocardial ischemia. International journal of cardiology 34 14975559
1988 A polypeptide growth inhibitor isolated from lactating bovine mammary gland (MDGI) is a lipid-carrying protein. Journal of cellular biochemistry 33 3230093
2013 Increased H-FABP concentrations in nonalcoholic fatty liver disease. Possible marker for subclinical myocardial damage and subclinical atherosclerosis. Herz 32 23324907
1996 The human mammary-derived growth inhibitor (MDGI) gene: genomic structure and mutation analysis in human breast tumors. Genomics 30 8661024
2020 SERS-based magnetic immunoassay for simultaneous detection of cTnI and H-FABP using core-shell nanotags. Analytical methods : advancing methods and applications 29 33165490
2010 Heart fatty acid binding protein (H-FABP) as a diagnostic biomarker in patients with acute coronary syndrome. Heart, lung & circulation 29 20674495
2018 FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase67 Promoter Region. The Journal of neuroscience : the official journal of the Society for Neuroscience 28 30341178
2016 High-resolution neutron and X-ray diffraction room-temperature studies of an H-FABP-oleic acid complex: study of the internal water cluster and ligand binding by a transferred multipolar electron-density distribution. IUCrJ 26 27006775
2007 The fabp4 gene of zebrafish (Danio rerio)--genomic homology with the mammalian FABP4 and divergence from the zebrafish fabp3 in developmental expression. The FEBS journal 26 17480210
2014 Diagnostic value of analysis of H-FABP, NT-proBNP, and cTnI in heart function in children with congenital heart disease and pneumonia. European review for medical and pharmacological sciences 25 24899611
2014 Overexpression of FABP3 inhibits human bone marrow derived mesenchymal stem cell proliferation but enhances their survival in hypoxia. Experimental cell research 24 24583397
2006 FABP3 and FABP10 in Atlantic salmon (Salmo salar L.)--general effects of dietary fatty acid composition and life cycle variations. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 24 16905349
2020 Expression of the Novel Cardiac Biomarkers sST2, GDF-15, suPAR, and H-FABP in HFpEF Patients Compared to ICM, DCM, and Controls. Journal of clinical medicine 23 32326570
2024 The oxidized phospholipid PGPC impairs endothelial function by promoting endothelial cell ferroptosis via FABP3. Journal of lipid research 22 38218337
2017 Influences of Ivabradine treatment on serum levels of cardiac biomarkers sST2, GDF-15, suPAR and H-FABP in patients with chronic heart failure. Acta pharmacologica Sinica 22 29239349
2014 Distribution of H-FABP and ACSL4 gene polymorphisms and their associations with intramuscular fat content and backfat thickness in different pig populations. Genetics and molecular research : GMR 22 25177956
2012 The evolutionary relationship of the transcriptionally active fabp11a (intronless) and fabp11b genes of medaka with fabp11 genes of other teleost fishes. The FEBS journal 22 22520026
2022 FABP3 overexpression promotes vascular fibrosis in Takayasu's arteritis by enhancing fatty acid oxidation in aorta adventitial fibroblasts. Rheumatology (Oxford, England) 21 34718429
2013 Tissue-specific transcriptional modulation of fatty acid-binding protein genes, fabp2, fabp3 and fabp6, by fatty acids and the peroxisome proliferator, clofibrate, in zebrafish (Danio rerio). Gene 21 23466978
2013 Knockdown of FABP3 impairs cardiac development in Zebrafish through the retinoic acid signaling pathway. International journal of molecular sciences 21 23823803
2012 Investigation of four porcine candidate genes (H-FABP, MYOD1, UCP3 and MASTR) for meat quality traits in Large White pigs. Molecular biology reports 21 22311016
2012 Diagnostic accuracy of heart fatty acid binding protein (H-FABP) and glycogen phosphorylase isoenzyme BB (GPBB) in diagnosis of acute myocardial infarction in patients with acute coronary syndrome. Biochemia medica 21 22838188
2012 Association between subcutaneous and intramuscular fat content in porcine ham and loin depending on age, breed and FABP3 and LEPR genes transcript abundance. Molecular biology reports 21 23192618
1989 Response of different mammary epithelial cell lines to a mammary derived growth inhibitor (MDGI). Biomedica biochimica acta 21 2775246
2016 miR-192-5p mediates hypoxia/reoxygenation-induced apoptosis in H9c2 cardiomyocytes via targeting of FABP3. Journal of biochemical and molecular toxicology 20 27780314
2014 Heart-type fatty-acid-binding protein (FABP3) is a lysophosphatidic acid-binding protein in human coronary artery endothelial cells. FEBS open bio 20 25426414
2013 FABP3 and brown adipocyte-characteristic mitochondrial fatty acid oxidation enzymes are induced in beige cells in a different pathway from UCP1. Biochemical and biophysical research communications 20 24129192
2012 Silencing of FABP3 promotes apoptosis and induces mitochondrion impairment in embryonic carcinoma cells. Journal of bioenergetics and biomembranes 20 22528395
2011 Heart-type fatty acid binding protein (H-FABP): relationship with arterial intima-media thickness and role as diagnostic marker for atherosclerosis in patients with ımpaired glucose metabolism. Cardiovascular diabetology 20 21535886
2000 Breast cancer growth inhibition by delivery of the MDGI-derived peptide P108. Oncogene 20 10828888
1995 HH2A, an immortalized bovine mammary epithelial cell line, expresses the gene encoding mammary derived growth inhibitor (MDGI). In vitro cellular & developmental biology. Animal 20 7535635
2013 Silencing of FABP3 leads to apoptosis-induced mitochondrial dysfunction and stimulates Wnt signaling in zebrafish. Molecular medicine reports 19 23846528
2012 Developmental changes and effect on intramuscular fat content of H-FABP and A-FABP mRNA expression in pigs. Journal of applied genetics 19 23135696
2015 Pretreatment levels of the fatty acid handling proteins H-FABP and CD36 predict response to olanzapine in recent-onset schizophrenia patients. Brain, behavior, and immunity 18 26541453
2013 Silencing of FABP3 inhibits proliferation and promotes apoptosis in embryonic carcinoma cells. Cell biochemistry and biophysics 18 23097025
2012 Plasma levels of FABP4, but not FABP3, are associated with increased risk of diabetes. Lipids 18 22706792
2003 Polymorphisms in fatty acid-binding protein-3 (FABP3) - putative association with type 2 diabetes mellitus. Human mutation 18 12872269
2004 Phenotype of palmitic acid transport and of signalling in alveolar type II cells from E/H-FABP double-knockout mice: contribution of caveolin-1 and PPARgamma. Biochimica et biophysica acta 17 15164767
1990 Differentiation-promoting effects of mammary-derived growth inhibitor (MDGI) on pluripotent mouse embryonic stem cells. Virchows Archiv. B, Cell pathology including molecular pathology 17 1981402
2023 A self-powered photoelectrochemical biosensing platform for H-FABP monitoring mediated by CsPbBr3@COF-V. Biosensors & bioelectronics 16 37769486
2016 Association of H-FABP gene polymorphisms with intramuscular fat content in Three-yellow chickens and Hetian-black chickens. Journal of animal science and biotechnology 16 26900465
2006 Clinical evaluation of point-of-care-testing of heart-type fatty acid-binding protein (H-FABP) for the diagnosis of acute myocardial infarction. Journal of immunoassay & immunochemistry 16 16827225
2002 A study of associations of the H-FABP genotypes with fat and meat production of pigs. Journal of applied genetics 16 12441635
2022 Effect of Mulberry Leaf TMR on Growth Performance, Meat Quality and Expression of Meat Quality Master Genes (ADSL, H-FABP) in Crossbred Black Goats. Foods (Basel, Switzerland) 15 36553774
2021 Fatty Acid Binding Protein 3 (FABP3) and Apolipoprotein E4 (ApoE4) as Lipid Metabolism-Related Biomarkers of Alzheimer's Disease. Journal of clinical medicine 15 34300173
2015 Heart-type fatty acid binding protein (H-FABP) in patients in an emergency department setting, suspected of acute coronary syndrome: optimal cut-off point, diagnostic value and future opportunities in primary care. The European journal of general practice 15 25751665
2014 Preoperative serum h-FABP concentration is associated with postoperative incidence of acute kidney injury in patients undergoing cardiac surgery. BMC cardiovascular disorders 15 25212385
2006 The developmental changes and effect on IMF content of H-FABP and PPARgamma mRNA expression in sheep muscle. Yi chuan xue bao = Acta genetica Sinica 15 16800381
2006 Relationship among H-FABP gene polymorphism, intramuscular fat content, and adipocyte lipid droplet content in main pig breeds with different genotypes in western China. Yi chuan xue bao = Acta genetica Sinica 15 16800382
2023 Plasma Galectin-3 and H-FABP correlate with poor physical performance in patients with congestive heart failure. Experimental biology and medicine (Maywood, N.J.) 14 36803120
1991 Modulation of the beta-adrenergic-response in cultured rat heart cells. II. Mammary-derived growth inhibitor (MDGI) blocks induction of beta-adrenergic supersensitivity. Dissociation from lipid-binding activity of MDGI. Molecular and cellular biochemistry 14 1646956
2022 miR-381-3p Inhibits Intramuscular Fat Deposition through Targeting FABP3 by ceRNA Regulatory Network. Biology 13 36290402
2016 Tissue expression analysis, cloning and characterization of the 5'-regulatory region of the bovine FABP3 gene. Molecular biology reports 13 27270359
2004 Association of the heart fatty acid-binding protein (FABP3) gene with milk traits in Manchega breed sheep. Animal genetics 13 15265079
2024 Combinatorial immune checkpoint blockade increases myocardial expression of NLRP-3 and secretion of H-FABP, NT-Pro-BNP, interleukin-1β and interleukin-6: biochemical implications in cardio-immuno-oncology. Frontiers in cardiovascular medicine 12 38322766
2024 Fatty acid binding to the human transport proteins FABP3, FABP4, and FABP5 from a Ligand's perspective. The Journal of biological chemistry 12 38777142
2022 TGF-β1 stimulated mesenchymal stem cells-generated exosomal miR-29a promotes the proliferation, migration and fibrogenesis of tenocytes by targeting FABP3. Cytokine 12 36481477
2010 H-FABP in cases of carbon monoxide intoxication admitted to the emergency room. Human & experimental toxicology 12 21075804
2000 Deletion of the gene encoding H-FABP/MDGI has no overt effects in the mammary gland. Transgenic research 12 11206972
2024 FABP3 Induces Mitochondrial Autophagy to Promote Neuronal Cell Apoptosis in Brain Ischemia-Reperfusion Injury. Neurotoxicity research 11 39008165
2024 Heart-type fatty acid binding protein (H-FABP) as an early biomarker in sepsis-induced cardiomyopathy: a prospective observational study. Lipids in health and disease 11 39232765
2021 Suppression of α-synuclein propagation after intrastriatal injection in FABP3 null mice. Brain research 11 33636166
2020 Tumor-Targeting Peptides: The Functional Screen of Glioblastoma Homing Peptides to the Target Protein FABP3 (MDGI). Cancers 11 32650473

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