Affinage

EMILIN1

EMILIN-1 · UniProt Q9Y6C2

Length
1016 aa
Mass
106.7 kDa
Annotated
2026-06-09
85 papers in source corpus 38 papers cited in narrative 38 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 9/9 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

EMILIN-1 is a homotrimeric extracellular matrix glycoprotein that localizes at the elastin-microfibril interface of elastic fibers, where it controls elastogenesis, vascular and lymphatic architecture, and cell proliferation (PMID:8458869, PMID:14701737). Its modular architecture comprises an N-terminal cysteine-rich EMI domain, a coiled-coil/leucine-zipper stalk, a collagenous region, and a C-terminal gC1q globular domain; the gC1q and collagenous domains drive self-assembly into homotrimers and large disulfide-bonded multimers (PMID:10358019, PMID:10821830). EMILIN-1 organizes the elastic fiber matrix by incorporating into fibrillin microfibril networks (in a fibrillin-1- and fibronectin-dependent manner) and by directly binding fibulin-4/EFEMP2 to enable its matrix deposition, LOX activity, and collagen crosslinking, thereby coupling elastogenesis to collagen fibrillogenesis (PMID:26945878, PMID:28717224, PMID:36351433). The gC1q domain adopts a nine-stranded β-sandwich bearing a unique disordered apical loop that exposes Glu933; all three E933 residues of the trimer coordinate the integrin MIDAS to engage α4β1 and α9β1 integrins, mediating cell adhesion and migration (PMID:18463100, PMID:29037761, PMID:12456677). Through this gC1q–integrin engagement, EMILIN-1 inhibits cell proliferation by silencing the RAS-ERK pathway via ubiquitination of HRas-GTP and by reducing PTEN, an effect requiring the intact α4 integrin cytoplasmic tail (PMID:28177903, PMID:21949412). EMILIN-1 also acts as a negative regulator of TGF-β by inhibiting pro-TGF-β proteolysis, and smooth-muscle-cell-derived EMILIN-1 controls resting blood pressure by restraining arteriolar myogenic tone through a TGF-β→HB-EGF→EGFR→TRPC6/TRPM4 cascade (PMID:16530037, PMID:22814752, PMID:30354220). The same integrin interaction maintains lymphatic vessel patterning, valve formation, and lymphangiogenesis (PMID:18411305, PMID:24019067, PMID:30408617). Neutrophil elastase cleaves EMILIN-1 within the gC1q domain at the S913-R914 bond, disrupting its conformation and abolishing α4β1 binding and anti-proliferative activity, a mechanism operative in tumors and lymphedema (PMID:28074935, PMID:24513040, PMID:26920215). Heterozygous and bi-allelic EMILIN-1 mutations cause autosomal connective tissue disorders with impaired secretion or matrix deposition and disorganized fibrillar architecture (PMID:26462740, PMID:36351433, PMID:31978608).

Mechanistic history

Synthesis pass · year-by-year structured walk · 37 steps
  1. 1993 High

    Established EMILIN-1 as a structural constituent of elastic fibers with a functional role in elastogenesis, the founding observation for the gene.

    Evidence Immunoelectron microscopy with quantitative gold-particle analysis and antibody inhibition of elastin deposition in aorta cell culture

    PMID:8458869

    Open questions at the time
    • Molecular partners mediating elastin deposition not yet defined
    • Domain responsible for elastogenic activity unknown at this stage
  2. 1999 High

    Defined the multidomain architecture and showed the gC1q domain mediates cell adhesion, framing EMILIN-1 as both a structural and cell-interactive ECM protein.

    Evidence cDNA cloning, CD spectroscopy, recombinant gC1q expression and cell adhesion assay

    PMID:10358019

    Open questions at the time
    • Adhesion receptor not yet identified
    • Functional roles of EMI and coiled-coil domains undefined
  3. 2000 High

    Resolved how EMILIN-1 oligomerizes, showing the gC1q and collagenous domains drive homotrimer and high-order multimer assembly.

    Evidence Yeast two-hybrid, native PAGE, and truncation constructs

    PMID:10821830

    Open questions at the time
    • Stoichiometry of disulfide-bonded multimers not fully mapped
    • Relationship between assembly state and matrix incorporation unresolved
  4. 2002 High

    Identified α4β1 integrin as the gC1q adhesion receptor, establishing the cell-surface link that underlies EMILIN-1's signaling functions.

    Evidence Function-blocking mAbs, K562 α4-transfection gain-of-function, and actin imaging

    PMID:12456677

    Open questions at the time
    • Precise integrin-binding residue not yet mapped
    • Downstream signaling consequences unexplored
  5. 2004 High

    In vivo knockout confirmed EMILIN-1's requirement for elastic fiber formation and vascular cell anchorage.

    Evidence Emilin1-/- mouse histology, electron microscopy, and fibroblast culture

    PMID:14701737

    Open questions at the time
    • Molecular pathway linking loss to phenotype not yet defined
    • No physiological vascular readout (blood pressure) examined
  6. 2006 High

    Connected EMILIN-1 to TGF-β biology and blood pressure, showing it restrains pro-TGF-β proteolysis to limit TGF-β bioavailability.

    Evidence Emilin1-/- mouse blood pressure measurement and anti-TGF-β antibody rescue

    PMID:16530037 PMID:22814752

    Open questions at the time
    • Mechanism of pro-TGF-β proteolysis inhibition not biochemically resolved
    • Cellular source of functional EMILIN-1 not yet identified
  7. 2006 High

    Demonstrated the α4β1-gC1q axis drives directed cell migration in a physiological tissue context (trophoblast invasion).

    Evidence Haptotaxis assays with EVT cells, function-blocking mAbs, siRNA, and MMP co-culture assays

    PMID:17074837

    Open questions at the time
    • Whether migration vs. proliferation outcomes depend on cell type unresolved
    • Signaling downstream of α4β1 not dissected
  8. 2008 High

    Solved the gC1q structure and pinpointed Glu933 in a disordered apical loop as the essential integrin-binding residue, providing the structural basis for EMILIN-1's cell-interactive functions.

    Evidence TROSY NMR structure, E933A mutagenesis, and adhesion/migration assays under static and flow conditions

    PMID:18463100

    Open questions at the time
    • Atomic-level integrin docking geometry not yet defined
    • Whether E933 mediates α9β1 as well as α4β1 not yet tested
  9. 2008 High

    Extended EMILIN-1 function to the lymphatic system, identifying it as a regulator of lymphatic vessel growth and integrity.

    Evidence Emilin1-/- mouse lymphangiography, ultrastructure, and lymph drainage assays across two backgrounds

    PMID:18411305

    Open questions at the time
    • Receptor and signaling pathway for lymphatic phenotype not yet established
    • Distinction between valve and vessel defects unresolved
  10. 2011 High

    Established the anti-proliferative signaling mechanism, showing gC1q-α4β1/α9β1 engagement raises PTEN and dampens PI3K/Akt and Erk1/2 to restrain epidermal/dermal proliferation.

    Evidence Emilin1-/- skin, function-blocking anti-α4/α9 mAbs, and phospho-pathway immunoblots

    PMID:21949412

    Open questions at the time
    • Direct mechanism connecting integrin to PTEN levels undefined
    • How Erk1/2-Smad2 crosstalk is initiated unresolved
  11. 2012 High

    Localized the functional source of blood-pressure control to smooth muscle cell EMILIN-1 acting through suppression of arteriolar myogenic tone.

    Evidence Cell-type-specific transgenic rescue (SMC vs. endothelial) and anti-TGF-β treatment with ex vivo myogenic tone measurement

    PMID:22814752

    Open questions at the time
    • Downstream effectors of TGF-β in SMC tone not yet identified
    • Channel-level mechanism of myogenic tone unresolved
  12. 2012 Medium

    Showed EMILIN-1 can self-assemble through gC1q and EMI domains and form hetero-multimers with EMILIN-2, expanding the matrix-assembly model.

    Evidence Yeast two-hybrid, co-IP from co-transfected cells, and immunofluorescence colocalization

    PMID:25445627

    Open questions at the time
    • Yeast two-hybrid/co-IP not reconstituted with purified proteins
    • Functional significance of EMILIN-1/2 heteromers unknown
  13. 2012 Medium

    Reported an unexpected intracellular interaction between the EMILIN-1 C-terminus and the CNGA3 channel in hair cells.

    Evidence Yeast two-hybrid, surface plasmon resonance with Ca2+-dependent kinetics, and pull-down in organ of Corti systems

    PMID:22248097

    Open questions at the time
    • Intracellular interaction context unusual for a secreted ECM protein and not independently confirmed
    • Physiological consequence in hearing not established
  14. 2010 Medium

    Demonstrated EMILIN-1 is required for fibrillin-1 microfibril deposition, linking it to oxytalan fiber assembly.

    Evidence Co-IP and siRNA knockdown reducing fibrillin-1 deposition in periodontal ligament cells

    PMID:20701466

    Open questions at the time
    • Direct binding not distinguished from indirect assembly dependence
    • Single cell system
  15. 2014 High

    Identified neutrophil elastase cleavage as a pathological inactivation mechanism that abolishes EMILIN-1's anti-proliferative function in tumors.

    Evidence In vitro NE cleavage, fragment analysis, proliferation assays, and tumor specimen immunostaining

    PMID:24513040

    Open questions at the time
    • Cleavage site not yet mapped at this stage
    • Causality in tumor progression in vivo not established
  16. 2014 Medium

    Distinguished canonical and non-canonical TGF-β contributions to EMILIN-1-deficient aortic valve disease over time.

    Evidence Emilin1-/- mouse histology, immunoblotting, and echocardiography

    PMID:25056700

    Open questions at the time
    • Pathway assignment based on correlative immunoblot
    • Single lab; causal manipulation of pSmad vs pErk not performed
  17. 2015 Medium

    First human disease link: a heterozygous p.A22T mutation near the signal peptide impairs secretion, causing ER retention and an autosomal-dominant connective tissue disorder.

    Evidence Trio exome sequencing, familial segregation, mutant transfection ER-retention assay, and patient skin biopsy

    PMID:26462740

    Open questions at the time
    • Single family
    • Mechanism connecting reduced deposition to tissue phenotype not dissected
  18. 2016 High

    Defined the assembly hierarchy placing EMILIN-1 within fibrillin microfibril networks dependent on fibrillin-1 and fibronectin scaffolds.

    Evidence Immunoelectron microscopy, fibrillin-1-deficient mouse skin, and wound-healing matrix assembly

    PMID:26945878

    Open questions at the time
    • Order of recruitment among EMILIN-1, fibronectin, and fibrillin not fully resolved
    • Direct binding interfaces not mapped
  19. 2016 High

    Linked NE-mediated EMILIN-1 degradation to lymphedema pathogenesis and showed pharmacological NE inhibition is protective.

    Evidence Mouse tail lymphedema model with sivelestat treatment, EMILIN-1 integrity immunoblot, and human biopsy validation

    PMID:26920215

    Open questions at the time
    • How EMILIN-1 loss weakens LEC junctions mechanistically unresolved
    • Cleavage site not yet defined here
  20. 2017 High

    Mapped the precise NE cleavage site (S913-R914) within gC1q and showed an NE-resistant R914W mutant preserves integrin binding and anti-proliferative activity, defining the molecular basis of functional inactivation.

    Evidence Comparative in vitro protease assays (NE, MMP-3, MMP-9, MT1-MMP), R914W mutagenesis, and adhesion/proliferation assays

    PMID:28074935

    Open questions at the time
    • In vivo relevance of R914W resistance not tested
    • Why MMP cleavage spares gC1q function not structurally explained
  21. 2017 High

    Resolved the anti-proliferative signaling node, showing gC1q-α4β1 engagement drives ubiquitination of HRas-GTP requiring the α4 cytoplasmic tail.

    Evidence gC1q plating, HRas-GTP/ubiquitination pull-down, co-IP, α4 truncation mutants, and salirasib pharmacology

    PMID:28177903

    Open questions at the time
    • Identity of the ubiquitin ligase acting on HRas unknown
    • Link to PTEN regulation not unified
  22. 2017 High

    Showed EMILIN-1 selectively governs fibulin-4 matrix deposition through direct binding, distinguishing it from effects on other fibulins or LTBPs.

    Evidence RNAi in osteoblasts, immunoprecipitation, and ELISA-style binding assay

    PMID:28717224

    Open questions at the time
    • Binding interface between EMILIN-1 and fibulin-4 not mapped
    • Connection to downstream LOX/collagen biology not yet made here
  23. 2017 Medium

    Provided an atomic model of the gC1q-α4β1 interface, showing all three trimeric E933 residues coordinate the MIDAS Mg2+ with R904 as a synergistic residue.

    Evidence Site-directed mutagenesis, adhesion assays, and docking of the NMR structure onto a virtual α4β1 model

    PMID:29037761

    Open questions at the time
    • MIDAS coordination model is computational
    • No co-crystal or cryo-EM structure of the complex
  24. 2018 High

    Defined the SMC blood-pressure mechanism in adults, mapping TGF-β→HB-EGF→EGFR→TRPC6/TRPM4 channel recruitment that potentiates myogenic tone, with human hypertensive validation.

    Evidence Conditional SMC-specific Emilin1 knockout, ex vivo myogenic tone, signaling immunoblots, pharmacological inhibition, and patient biopsy

    PMID:30354220

    Open questions at the time
    • Direct biochemical link from TGF-β to HB-EGF induction not fully resolved
    • Whether the same cascade operates in non-resistance vessels untested
  25. 2018 High

    Demonstrated the gC1q/E933-integrin interaction is required for normal lymphangiogenesis and restrains lymph node metastasis using a point-mutant model.

    Evidence E933A knock-in mouse, lymphangiography, ex vivo thoracic duct ring assay, and metastasis assay

    PMID:30408617

    Open questions at the time
    • Signaling pathway in LECs downstream of integrin not dissected
    • Mechanism linking lymphatic dysfunction to metastasis unresolved
  26. 2019 High

    Showed EMILIN-1-integrin interaction restrains colitis-associated carcinogenesis and maintains inflammatory-cell lymphatic drainage.

    Evidence AOM-DSS carcinogenesis in Emilin1-/- and E933A mice with histopathology and lymphatic functional assessment

    PMID:31479698

    Open questions at the time
    • Whether tumor suppression is cell-autonomous or microenvironmental unresolved
    • Specific integrin-bearing cells responsible not identified
  27. 2019 Medium

    Identified a TSPAN9 interaction through which EMILIN-1 enhances suppression of gastric cancer cell migration and invasion.

    Evidence Co-IP, immunofluorescence, and overexpression/knockdown functional assays

    PMID:31242895

    Open questions at the time
    • Single Co-IP without reciprocal validation in a second system
    • Whether interaction is direct unknown
  28. 2019 Medium

    Documented EMILIN-1 associations with elastin and collagen IV in skin fibroblasts and downstream effects on FGFR2 and COL6A1.

    Evidence Co-IP, multiphoton/confocal colocalization, and siRNA with expression analysis

    PMID:30843221

    Open questions at the time
    • Direct vs. matrix-mediated association not distinguished
    • Single lab
  29. 2019 Medium

    Showed ECM-deposited EMILIN-1 sets adhesion strength and focal adhesion/stress fiber content in renal epithelial cells while permitting migration.

    Evidence siRNA knockdown of ECM EMILIN-1, adhesion strength assay, focal adhesion staining, and migration assay

    PMID:33543009

    Open questions at the time
    • Receptor mediating renal epithelial adhesion not identified
    • Single lab
  30. 2020 Medium

    Extended human disease to a coiled-coil p.R250C mutation with a zebrafish model revealing a role in peripheral nervous system/motor neuron development.

    Evidence Exome sequencing, patient skin/fibroblast immunofluorescence, and zebrafish morpholino knockdown with wild-type vs. mutant cRNA rescue

    PMID:31978608

    Open questions at the time
    • Mechanism linking EMILIN-1 to motor neuron arborization unresolved
    • Morpholino-based; single family
  31. 2021 Medium

    Showed in chronic lymphocytic leukemia the intact gC1q-VLA-4 interaction can be pro-survival, with EMILIN-1 remaining uncleaved due to absence of NE.

    Evidence Adhesion assays, phospho-ERK/AKT immunoblots, apoptosis assays, and bone marrow NE immunostaining

    PMID:34783040

    Open questions at the time
    • Context-dependent switch between anti-proliferative and pro-survival signaling unexplained
    • Single lab
  32. 2021 Medium

    Identified proteolysis and EV packaging as a mechanism by which melanoma cells lower intracellular EMILIN-1 to evade its anti-tumor properties.

    Evidence Small EV proteomics, overexpression/knockdown, migration/viability assays, and mouse tumor/metastasis model

    PMID:34299025

    Open questions at the time
    • Protease responsible for EV-associated cleavage not identified
    • Mechanism of EV sorting unknown
  33. 2022 High

    Unified elastogenesis with collagen biology, showing EMILIN-1 loss impairs fibulin-4 deposition and LOX activity, reducing collagen crosslinking and causing bone phenotypes in humans and mice.

    Evidence Human bi-allelic variants, Emilin1-/- mice, EFEMP2 deposition, LOX activity, crosslinking biochemistry, electron microscopy, and bone histomorphometry

    PMID:36351433

    Open questions at the time
    • Whether EMILIN-1 directly modulates LOX or acts via fibulin-4 not separated
    • Tissue specificity of bone phenotype not fully explained
  34. 2022 Medium

    Connected EMILIN-1 loss to inflammatory skin disease, showing both EMI and gC1q domains shape macrophage recruitment and non-canonical TGF-β signaling in psoriasis.

    Evidence IMQ psoriasis in Emilin1-/- and E933A mice, flow cytometry, TGF-β immunoblotting, and human lesion immunostaining

    PMID:35764213

    Open questions at the time
    • Separate contributions of EMI vs gC1q domains not cleanly resolved
    • Single lab
  35. 2023 Medium

    Showed EMILIN-1 promotes proliferation and osteo/odontogenic differentiation of dental pulp stem cells.

    Evidence siRNA knockdown and recombinant EMILIN-1 addition with proliferation, mineralization, and differentiation marker assays

    PMID:37024847

    Open questions at the time
    • Receptor and signaling pathway in hDPSCs not identified
    • Apparent pro-proliferative role contrasts with anti-proliferative integrin signaling and is unreconciled
  36. 2025 Medium

    Demonstrated tumor-suppressive activity in HNSCC and cancer-associated fibroblasts through downregulation of cell cycle and aurora kinase signaling.

    Evidence Overexpression/knockdown, co-culture, RNA-seq, immunoblotting, and in ovo CAM tumor model with Ki-67/cleaved caspase-3 staining

    PMID:39892781

    Open questions at the time
    • Whether the aurora kinase axis links to the gC1q-integrin pathway unresolved
    • Single lab
  37. 2025 Medium

    Showed extravillous trophoblast-secreted EMILIN-1 acts as a TGF-β antagonist that reverses decidual myofibroblast activation and resistance to invasion.

    Evidence EVT conditioned medium assays, TGF-β signaling readouts, collagen production, and invasion assays

    PMID:39756583

    Open questions at the time
    • Molecular basis of TGF-β antagonism by secreted EMILIN-1 not defined
    • Single lab

Open questions

Synthesis pass · forward-looking unresolved questions
  • How EMILIN-1 mechanistically inhibits pro-TGF-β proteolysis, and how the same gC1q-integrin interaction yields opposite (anti-proliferative vs pro-survival) outcomes across cell contexts, remains unresolved.
  • No biochemical mechanism for pro-TGF-β proteolysis inhibition
  • Context-dependent signaling switch unexplained
  • No experimental high-resolution structure of the gC1q-integrin complex

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 4 GO:0060089 molecular transducer activity 3 GO:0098631 cell adhesion mediator activity 3 GO:0098772 molecular function regulator activity 3
Localization
GO:0031012 extracellular matrix 5 GO:0005576 extracellular region 3 GO:0005783 endoplasmic reticulum 1
Pathway
R-HSA-1474244 Extracellular matrix organization 5 R-HSA-1643685 Disease 4 R-HSA-162582 Signal Transduction 3 R-HSA-1430728 Metabolism 1
Partners
CNGA3EFEMP2EMILIN2FBN1HRASITGA4ITGA9TSPAN9
Complex memberships
elastic fiberfibrillin microfibril network

Evidence

Reading pass · 38 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1993 EMILIN-1 (then called 'emilin') localizes preferentially at the elastin-microfibril interface within elastic fibers, and anti-emilin antibodies added to culture medium severely alter elastin deposition by aorta cells, demonstrating a direct role in elastogenesis. Immunoelectron microscopy (post- and pre-embedding immunolabeling), quantitative gold-particle distribution analysis, and antibody inhibition in cell culture The Journal of cell biology High 8458869
1999 Human EMILIN-1 encodes a 1016-aa protein with a C-terminal gC1q-like globular domain (adopting high β-sheet content by CD), a collagenous stalk, coiled-coil/leucine-zipper region, and an N-terminal cysteine-rich (EMI) domain; the recombinant gC1q domain promotes cell adhesion. cDNA cloning, RT-PCR, peptide sequencing, CD spectroscopy, recombinant protein expression, cell adhesion assay The Journal of biological chemistry High 10358019
2000 The EMILIN-1 gC1q domain self-assembles into homotrimers (demonstrated by yeast two-hybrid and native gel migration) and the full-length protein forms large disulfide-bonded multimers of many millions of daltons; the C-terminal gC1q and collagenous domains are essential for trimer formation. Yeast two-hybrid, native PAGE, reducing/non-reducing agarose gels, recombinant domain expression, truncation constructs The Journal of biological chemistry High 10821830
2002 Cell adhesion to EMILIN-1 is mediated by the gC1q domain engaging the α4β1 integrin; a function-blocking anti-gC1q mAb completely blocks attachment, and only α4-transfected K562 cells (not wild-type K562) adhere to EMILIN-1. Cells on EMILIN-1 show ruffled morphology without stress fibers, consistent with promotion of cell motility rather than strong adhesion. Monoclonal antibody inhibition assays, cell adhesion kinetics, adhesion-strength centrifugation, K562 α4-transfection, actin cytoskeleton imaging The Journal of biological chemistry High 12456677
2004 Emilin1-/- mice show abnormal elastic fiber formation in aorta and skin, and altered morphology and anchorage of endothelial and smooth muscle cells to elastic lamellae, demonstrating EMILIN-1's role in elastogenesis and vascular cell maintenance. Mouse gene targeting (knockout), histology, ultrastructural analysis by electron microscopy, embryonic fibroblast cultures Molecular and cellular biology High 14701737
2006 EMILIN-1 acts as a negative regulator of TGF-β processing and signaling in the vasculature; Emilin1-/- mice display elevated blood pressure due to increased TGF-β activity. EMILIN-1 inhibits pro-TGF-β proteolysis, limiting TGF-β bioavailability. Emilin1-/- mouse model, blood pressure measurement, vascular TGF-β signaling assays (referenced in Cell commentary citing Zacchigna et al. 2006 paper); rescue experiments with anti-TGF-β antibodies (PMID 22814752) Cell High 16530037 22814752
2006 EMILIN-1 in the uterine stroma promotes α4β1 integrin-dependent haptotactic migration of extravillous trophoblasts; function-blocking anti-α4 and anti-EMILIN-1 mAbs and EMILIN-1 siRNA each block trophoblast interaction with EMILIN-1/gC1q. Co-culture upregulates MT1-MMP and MMP-2. Haptotaxis assays with freshly isolated EVT and HTR-8/SVneo cells, function-blocking mAbs, siRNA knockdown, co-culture MMP assays Journal of cell science High 17074837
2008 The NMR solution structure of the EMILIN-1 gC1q homotrimer reveals a nine-stranded β-sandwich (missing one strand versus typical 10-stranded gC1q fold), with a unique disordered 19-residue protruding loop at the trimer apex; site-directed mutagenesis of Glu933 within this loop abrogates α4β1 integrin binding, T-cell adhesion under static and flow conditions, and endothelial cell haptotactic migration. NMR-based homology modeling, TROSY NMR, site-directed mutagenesis (E933A), Jurkat T-cell and EA.hy926 endothelial cell adhesion assays under static and flow conditions The Journal of biological chemistry High 18463100
2008 Emilin1 deficiency leads to lymphatic vessel hyperplasia, enlargement, irregular patterning, reduction of anchoring filaments, enhanced lymphangioma formation, lymphedema, and compromised lymph drainage, identifying EMILIN-1 as a regulator of lymphatic vessel growth and integrity. Emilin1-/- mouse model (CD1 and C57BL/6 backgrounds), histology, ultrastructural analysis, lymphangiography, lymph drainage assays Molecular and cellular biology High 18411305
2011 EMILIN-1 directly engages α4β1 and α9β1 integrins via its gC1q domain (E933 residue) to inhibit dermal fibroblast and keratinocyte proliferation; loss of this interaction in Emilin1-/- skin causes dermal/epidermal hyperproliferation via reduced PTEN, activation of PI3K/Akt and Erk1/2, and Erk1/2-mediated inhibitory phosphorylation of Smad2 at Ser245/250/255, suppressing TGF-β/Smad signaling. Emilin1-/- KO mouse, wound closure assays, function-blocking mAbs against α4 and α9 integrins, pathway analysis (phospho-immunoblots for Akt, Erk1/2, PTEN, Smad2), identification of EMILIN-1 as novel α9β1 ligand in keratinocytes The Journal of cell biology High 21949412
2012 Vascular smooth muscle cell-derived EMILIN-1 (not endothelial cell-derived) is the functional source controlling resting blood pressure; its mechanism involves suppression of arteriolar myogenic tone through restraint of TGF-β activity, as shown by TGF-β antibody normalization of BP in Emilin1-/- mice and selective SMC-restricted transgenic rescue. Cell-type-specific transgenic rescue in Emilin1-/- background (SMC vs. endothelial promoters), anti-TGF-β antibody treatment, ex vivo resistance artery myogenic tone measurement Arteriosclerosis, thrombosis, and vascular biology High 22814752
2013 EMILIN-1 interaction with α9β1 integrin, mediated by the E933 residue of the gC1q domain, is required for lymphatic valve formation and maintenance; mice expressing E933A-mutant EMILIN-1 (unable to engage α4/α9 integrins) display defective lymphatic valve structure and lymph flow, caused by deranged lymphatic endothelial cell proliferation and migration. Emilin1-/- KO mouse, E933A transgenic knock-in mouse, morphometric in vitro and in vivo assays, lymphangiography, cell proliferation and migration assays Molecular and cellular biology High 24019067
2014 Neutrophil elastase cleaves EMILIN-1 into three-to-four fragments, abolishing its anti-proliferative function; this cleavage mechanism is operative in sarcomas and ovarian cancers where PMN infiltration and elastase positivity are detected. In vitro protease cleavage assay with neutrophil elastase, SDS-PAGE fragment analysis, proliferation assays with cleaved versus intact EMILIN-1, immunostaining of tumor specimens for elastase and EMILIN-1 Matrix biology High 24513040
2014 In the Emilin1-/- aortic valve, early elastic fiber fragmentation is followed by aberrant angiogenesis and late fibrosis; constitutive canonical TGF-β (pSmad2/3) signaling is upregulated from birth, while non-canonical TGF-β (pErk1/2 MAPK) signaling progressively increases, mediating advanced fibrotic disease. Emilin1-/- mouse model, histology, immunohistochemistry, electron microscopy, quantitative gene expression, immunoblotting, echocardiography Disease models & mechanisms Medium 25056700
2015 A heterozygous missense mutation p.A22T in EMILIN-1 (mapping near the signal peptide cleavage site) causes impaired protein secretion with accumulation in the endoplasmic reticulum, reduced extracellular deposition, and disorganized fibrillar architecture in patient skin, segregating with an autosomal-dominant connective tissue disorder. Trio exome sequencing, Sanger sequencing for familial segregation, transfection of mutant construct with ER localization assessment, skin biopsy immunostaining Human mutation Medium 26462740
2016 EMILIN-1 and EMILIN-2 are incorporated into fibrillin microfibril networks in skin; fibrillin-1 deficiency disrupts EMILIN-1/2 networks. EMILIN-1/2 deposition during wound healing also requires fibronectin as an assembly scaffold. Immunoelectron microscopy, immunofluorescence, biochemical extraction, analysis of fibrillin-1-deficient mouse skin, wound healing model, embryonic fibroblast matrix assembly The Journal of investigative dermatology High 26945878
2016 In a mouse post-surgical tail lymphedema model, neutrophil elastase released by infiltrating neutrophils degrades EMILIN-1 in the acute phase, weakening lymphatic endothelial cell junctions and impairing drainage; local administration of the NE inhibitor sivelestat prevents EMILIN-1 cleavage and reduces lymphedema. Cleaved EMILIN-1 fragments with NE-dependent patterns are also detected in human secondary lymphedema. Mouse tail lymphedema model, NE inhibitor (sivelestat) treatment, EMILIN-1 integrity immunoblot, lymphatic junction analysis, lymph drainage assay, human lymphedema biopsy immunostaining Clinical science High 26920215
2017 Neutrophil elastase cleaves the EMILIN-1 gC1q domain at the S913-R914 peptide bond (outside the E933 integrin-binding site), disrupting gC1q structural conformation and abolishing α4β1 integrin interaction; the R914W mutant is resistant to NE cleavage and retains integrin-binding and anti-proliferative activity. MMP-3 and MT1-MMP partially cleave EMILIN-1 but do not impair gC1q-α4β1 function. In vitro protease cleavage assays (NE, MMP-3, MMP-9, MT1-MMP), site-directed mutagenesis (R914W NE-resistant mutant), cell adhesion and proliferation assays Scientific reports High 28074935
2017 EMILIN-1 gC1q silences the RAS-ERK proliferative pathway via α4β1 integrin through ubiquitination of HRas-GTP; α4 integrin co-immunoprecipitates ubiquitinated HRas only when ligated by gC1q, and this anti-proliferative effect requires the intact cytoplasmic tail of the α4 integrin chain. Cell plating on gC1q, phospho-ERK immunoblots, HRas-GTP pull-down with ubiquitinated HRas detection, co-immunoprecipitation, α4 truncation mutant transfection, salirasib (Ras inhibitor) pharmacology Oncotarget High 28177903
2017 EMILIN-1 depletion from osteoblasts by RNAi selectively abolishes fibulin-4 matrix deposition without affecting other fibulins or LTBPs; direct interaction between EMILIN-1 and fibulin-4 was confirmed by immunoprecipitation and ELISA-style binding assay. RNAi knockdown in primary calvarial osteoblasts and MC3T3-E1 cells, immunofluorescence, electron microscopy, immunoprecipitation, ELISA-style binding assay Scientific reports High 28717224
2017 The α4β1-EMILIN-1 gC1q interaction requires all three E933 residues of the homotrimer simultaneously; molecular docking of the NMR gC1q structure onto a virtual α4β1 model shows the three E933 residues coordinate the Mg²⁺ ion at the βI MIDAS site, and R904 acts as a synergistic residue via intra-chain salt bridges with E928/E930. Site-directed mutagenesis, cell adhesion assays, docking of NMR structure to virtual α4β1 crystal structure, structural modelling Matrix biology Medium 29037761
2018 Loss of smooth muscle cell EMILIN-1 in adult mice via conditional gene targeting increases blood pressure through enhanced arteriolar myogenic tone; mechanistically, higher TGF-β in smooth muscle cells drives HB-EGF expression and EGFR transactivation, which recruits TRPC6 and TRPM4 channels to potentiate voltage-dependent calcium channels and myogenic tone. Reduced EMILIN-1 and enhanced TGF-β-EGFR signaling are found in resistance arteries from hypertensive patients. Conditional SMC-specific Emilin1 knockout, ex vivo myogenic tone measurement, immunoblotting for TGF-β, HB-EGF, pEGFR, TRPC6/TRPM4 signaling, pharmacological EGFR and TRP channel inhibition, human hypertensive patient biopsy Arteriosclerosis, thrombosis, and vascular biology High 30354220
2018 The EMILIN-1 gC1q/E933-integrin interaction is required for normal lymphangiogenesis; E933A-mutant transgenic mice show abnormal dense, tortuous lymphatic networks, reduced anchoring filaments, aberrant narrowed collecting valves, impaired lymph transport, and enhanced lymph node metastasis. Lymphatic endothelial cells from E1-E933A mice have severely reduced sprouting capacity in ex vivo thoracic duct ring assays. E933A transgenic knock-in mouse, lymphangiography, morphometry, ex vivo thoracic duct ring assay, in vitro tubulogenesis assay, lymph node metastasis assay Matrix biology High 30408617
2010 EMILIN-1 is colocalized with fibrillin-1 in periodontal ligament cells, co-immunoprecipitates with fibrillin-1, and siRNA-mediated knockdown of EMILIN-1 reduces fibrillin-1 extracellular deposition to 23% of control, indicating EMILIN-1 regulates oxytalan fiber/microfibril formation. siRNA knockdown in PDL cell culture, immunofluorescence quantification of fibrillin-1 deposition, co-immunoprecipitation Connective tissue research Medium 20701466
2012 The EMILIN-1 gC1q and EMI domains can interact with each other and self-interact (gC1q-gC1q); full-length EMILIN-1 and EMILIN-2 can form non-covalent homo- and hetero-multimers, suggesting head-to-tail and tail-to-tail assemblies. Yeast two-hybrid (qualitative and quantitative), co-immunoprecipitation from co-transfected cell extracts, immunofluorescence co-localization Matrix biology Medium 25445627
2012 CNGA3 cyclic nucleotide-gated channel binds to the intracellular C-terminus domain of EMILIN-1; this interaction was identified by yeast two-hybrid and confirmed by surface plasmon resonance with Ca²⁺-dependent kinetics (Kd ~10⁻⁶–10⁻⁷ M) and pull-down assays in inner ear hair cell and organ of Corti systems. Yeast two-hybrid, surface plasmon resonance, pull-down assay, immunolocalization in saccular hair cells The Biochemical journal Medium 22248097
2019 Loss of EMILIN-1/β1 integrin interaction (via Emilin1-/- or E933A knock-in mice) promotes AOM-DSS colon tumor development with higher incidence and larger adenomas, increased lymphangiogenesis with dysfunctional lymphatics, and increased inflammatory infiltrates, demonstrating EMILIN-1-integrin interaction restrains colitis-associated carcinogenesis and maintains lymphatic drainage of inflammatory cells. AOM-DSS carcinogenesis protocol in Emilin1-/- and E1-E933A transgenic mice, histopathology, lymphatic vessel functional assessment, inflammatory cell scoring Matrix biology High 31479698
2019 Co-immunoprecipitation and immunofluorescence in gastric cancer cells demonstrate that EMILIN-1 and TSPAN9 interact and co-localize; EMILIN-1 overexpression boosts TSPAN9 expression and synergistically enhances the TSPAN9-dependent inhibition of gastric cancer cell migration and invasion. Co-immunoprecipitation, immunofluorescence co-localization, overexpression and knockdown functional assays (wound healing, invasion), Western blot BMC cancer Medium 31242895
2019 EMILIN-1 co-immunoprecipitates with elastin and collagen IV in human skin fibroblasts; it colocalizes with elastin, fibrillin-1, and collagen IV including at the basement membrane. siRNA knockdown of EMILIN-1 downregulates FGFR2 expression and COL6A1. Co-immunoprecipitation, multiphoton microscopy co-localization, confocal microscopy, siRNA knockdown with gene expression analysis International journal of cosmetic science Medium 30843221
2020 A heterozygous EMILIN-1 mutation p.R250C in the coiled-coil region causes reduced extracellular deposition with disorganized fibrillar network in patient skin and fibroblasts; zebrafish emilin1a knockdown produces locomotion defects and abnormal motor neuron axonal arborization, partially rescued by wild-type but not mutant EMILIN-1 cRNA, establishing a role in peripheral nervous system development. Whole exome sequencing, immunofluorescence of patient skin/fibroblasts, zebrafish morpholino knockdown, rescue by human wild-type vs. mutant EMILIN-1 cRNA injection, locomotion assay, motor neuron imaging Neurobiology of disease Medium 31978608
2022 Bi-allelic EMILIN-1 loss-of-function in humans and Emilin1-/- mice impairs EFEMP2 (fibulin-4) ECM deposition and LOX activity, resulting in reduced collagen crosslinking, aberrant collagen fibril ultrastructure, impaired elastogenesis, and abnormal trabecular bone formation, connecting elastic fiber assembly to collagen fibrillogenesis. Human genetics (bi-allelic variants), Emilin1-/- mouse analysis, EFEMP2 ECM deposition assay, LOX activity measurement, collagen crosslinking biochemistry, electron microscopy of collagen ultrastructure, bone histomorphometry American journal of human genetics High 36351433
2022 EMILIN-1 deficiency (null or E933A mutant) in psoriasis promotes macrophage recruitment, shifts polarization toward M1 via myofibroblast induction, and alters non-canonical TGF-β signaling through both the EMI and gC1q domains; the gC1q/integrin interaction controls skin homeostasis in keratinocytes and fibroblasts. IMQ-induced psoriasis in Emilin1-/- and E1-E933A mice, flow cytometry for macrophage subsets, immunoblotting for TGF-β pathway components, histopathology, human psoriatic lesion immunostaining Matrix biology Medium 35764213
2021 EMILIN-1 gC1q domain engages VLA-4 (α4β1) in CLL cells, promoting adhesion and pro-survival signaling (ERK and AKT phosphorylation) and impairment of in vitro apoptosis; the absence of neutrophil elastase in CLL bone marrow biopsies indicates EMILIN-1 remains structurally intact and functionally active in this context. Cell adhesion assays, phospho-ERK and phospho-AKT immunoblotting, apoptosis assay, immunohistochemistry of bone marrow biopsies, NE immunostaining Hematological oncology Medium 34783040
2021 In melanoma lymph node metastatic cells, EMILIN-1 is proteolyzed and packaged into small extracellular vesicles as a mechanism to reduce its intracellular levels; EMILIN-1 has intrinsic anti-proliferative, anti-migratory, and anti-tumor properties that are disabled by this proteolysis/EV secretion pathway. Small EV isolation and proteomics, EMILIN-1 overexpression and knockdown, migration assay, viability assay, primary tumor growth and metastasis in mouse model International journal of molecular sciences Medium 34299025
2023 EMILIN-1 knockdown reduces dental pulp stem cell (hDPSC) proliferation and osteo/odontogenic differentiation, while exogenous recombinant EMILIN-1 enhances both, establishing a functional role for EMILIN-1 in osteodifferentiation of hDPSCs. siRNA knockdown, exogenous rhEMILIN-1 addition, CCK-8 proliferation assay, ALP staining, alizarin red staining, qPCR and Western blot for differentiation markers BMC oral health Medium 37024847
2019 Depletion of EMILIN-1 from ECM of renal epithelial cells (by siRNA knockdown) reduces adhesion strength and decreases focal adhesion points and stress fibers without affecting cell growth, but promotes migration. siRNA knockdown of EMILIN-1 in ECM layer (novel cell-secreted ECM anchorage methodology), adhesion strength assay, focal adhesion immunostaining, migration assay Matrix biology plus Medium 33543009
2025 EMILIN-1 overexpression in HNSCC cells decreases proliferation, migration, and invasion, while EMILIN-1 knockdown in cancer-associated fibroblasts promotes these phenotypes; the anti-proliferative mechanism involves downregulation of cell cycle and aurora kinase signaling pathways, confirmed by RNA-sequencing and protein-level validation. In ovo CAM model confirms reduced tumor size, Ki-67, and increased cleaved caspase-3 with EMILIN-1 overexpression. Overexpression and siRNA knockdown, co-culture assay, conditioned medium assay, RNA-seq pathway analysis, immunoblotting, in ovo CAM tumor model, Ki-67 and cleaved caspase-3 immunostaining The American journal of pathology Medium 39892781
2025 Extravillous trophoblasts (EVTs) secrete EMILIN-1 (and Gremlin-1) as TGF-β antagonists that reverse decidual myofibroblast activation, reduce high collagen production, and functionally reverse acquired decidual resistance to trophoblast invasion. HTR8 and differentiated EVT from trophectoderm stem cells, conditioned medium assays, TGF-β signaling readouts, collagen production assays, trophoblast invasion assays Cells & development Medium 39756583

Source papers

Stage 0 corpus · 85 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2004 EMILIN-1 deficiency induces elastogenesis and vascular cell defects. Molecular and cellular biology 136 14701737
1993 Emilin, a component of elastic fibers preferentially located at the elastin-microfibrils interface. The Journal of cell biology 134 8458869
2008 Emilin1 deficiency causes structural and functional defects of lymphatic vasculature. Molecular and cellular biology 106 18411305
1993 Physiological analysis of mutants indicates involvement of the Saccharomyces cerevisiae GPI-anchored protein gp115 in morphogenesis and cell separation. Journal of bacteriology 100 8458831
2012 The EMILIN/Multimerin family. Frontiers in immunology 94 22566882
2000 The EMILIN protein family. Matrix biology : journal of the International Society for Matrix Biology 89 10963989
2011 EMILIN1-α4/α9 integrin interaction inhibits dermal fibroblast and keratinocyte proliferation. The Journal of cell biology 87 21949412
1991 Isolation and deduced amino acid sequence of the gene encoding gp115, a yeast glycophospholipid-anchored protein containing a serine-rich region. The Journal of biological chemistry 83 2061310
2002 beta 1 Integrin-dependent cell adhesion to EMILIN-1 is mediated by the gC1q domain. The Journal of biological chemistry 81 12456677
1999 EMILIN, a component of the elastic fiber and a new member of the C1q/tumor necrosis factor superfamily of proteins. The Journal of biological chemistry 71 10358019
2013 EMILIN1/α9β1 integrin interaction is crucial in lymphatic valve formation and maintenance. Molecular and cellular biology 64 24019067
2006 EMILIN1 represents a major stromal element determining human trophoblast invasion of the uterine wall. Journal of cell science 62 17074837
2000 Self-assembly and supramolecular organization of EMILIN. The Journal of biological chemistry 56 10821830
2001 Isolation and characterization of EMILIN-2, a new component of the growing EMILINs family and a member of the EMI domain-containing superfamily. The Journal of biological chemistry 50 11278945
2014 TGF-β mediates early angiogenesis and latent fibrosis in an Emilin1-deficient mouse model of aortic valve disease. Disease models & mechanisms 49 25056700
2001 Molecular cloning and characterization of EndoGlyx-1, an EMILIN-like multisubunit glycoprotein of vascular endothelium. The Journal of biological chemistry 49 11559704
2004 Overlapping, complementary and site-specific expression pattern of genes of the EMILIN/Multimerin family. Matrix biology : journal of the International Society for Matrix Biology 47 14996434
2012 EMILIN-3, peculiar member of elastin microfibril interface-located protein (EMILIN) family, has distinct expression pattern, forms oligomeric assemblies, and serves as transforming growth factor β (TGF-β) antagonist. The Journal of biological chemistry 43 22334695
2024 Cancer-associated fibroblast spatial heterogeneity and EMILIN1 expression in the tumor microenvironment modulate TGF-β activity and CD8+ T-cell infiltration in breast cancer. Theranostics 42 38505604
1990 The cell cycle modulated glycoprotein GP115 is one of the major yeast proteins containing glycosylphosphatidylinositol. Biochimica et biophysica acta 41 2160276
2016 Targeting of EMILIN-1 and EMILIN-2 to Fibrillin Microfibrils Facilitates their Incorporation into the Extracellular Matrix. The Journal of investigative dermatology 40 26945878
2015 Diagnostic Exome Sequencing Identifies a Novel Gene, EMILIN1, Associated with Autosomal-Dominant Hereditary Connective Tissue Disease. Human mutation 40 26462740
1994 O-linked oligosaccharides in yeast glycosyl phosphatidylinositol-anchored protein gp115 are clustered in a serine-rich region not essential for its function. The Journal of biological chemistry 40 8051047
2012 Vascular smooth muscle Emilin-1 is a regulator of arteriolar myogenic response and blood pressure. Arteriosclerosis, thrombosis, and vascular biology 38 22814752
2012 An EMILIN1-negative microenvironment promotes tumor cell proliferation and lymph node invasion. Cancer prevention research (Philadelphia, Pa.) 36 22827975
2019 TSPAN9 and EMILIN1 synergistically inhibit the migration and invasion of gastric cancer cells by increasing TSPAN9 expression. BMC cancer 33 31242895
2003 An emilin family extracellular matrix protein identified in the cochlear basilar membrane. Molecular and cellular neurosciences 33 12837629
2014 Neutrophil elastase-dependent cleavage compromises the tumor suppressor role of EMILIN1. Matrix biology : journal of the International Society for Matrix Biology 32 24513040
2019 Abrogation of EMILIN1-β1 integrin interaction promotes experimental colitis and colon carcinogenesis. Matrix biology : journal of the International Society for Matrix Biology 31 31479698
2000 Structure, chromosomal localization, and promoter analysis of the human elastin microfibril interfase located proteIN (EMILIN) gene. The Journal of biological chemistry 31 10625608
2008 The solution structure of EMILIN1 globular C1q domain reveals a disordered insertion necessary for interaction with the alpha4beta1 integrin. The Journal of biological chemistry 29 18463100
2002 Expression of the EMILIN-1 gene during mouse development. Matrix biology : journal of the International Society for Matrix Biology 29 12475644
2022 Colorectal cancer development is affected by the ECM molecule EMILIN-2 hinging on macrophage polarization via the TLR-4/MyD88 pathway. Journal of experimental & clinical cancer research : CR 27 35148799
1988 The elastin associated glycoprotein gp115. Synthesis and secretion by chick cells in culture. The Journal of biological chemistry 25 3182862
2018 Loss of EMILIN-1 Enhances Arteriolar Myogenic Tone Through TGF-β (Transforming Growth Factor-β)-Dependent Transactivation of EGFR (Epidermal Growth Factor Receptor) and Is Relevant for Hypertension in Mice and Humans. Arteriosclerosis, thrombosis, and vascular biology 24 30354220
2018 Loss of Multimerin-2 and EMILIN-2 Expression in Gastric Cancer Associate with Altered Angiogenesis. International journal of molecular sciences 24 30544909
2018 Integrin binding site within the gC1q domain orchestrates EMILIN-1-induced lymphangiogenesis. Matrix biology : journal of the International Society for Matrix Biology 22 30408617
2017 Neutrophil elastase cleavage of the gC1q domain impairs the EMILIN1-α4β1 integrin interaction, cell adhesion and anti-proliferative activity. Scientific reports 22 28074935
2016 Local inhibition of elastase reduces EMILIN1 cleavage reactivating lymphatic vessel function in a mouse lymphoedema model. Clinical science (London, England : 1979) 22 26920215
2010 Association study of the elastin microfibril interfacer 1 (EMILIN1) gene in essential hypertension. American journal of hypertension 22 20186130
2004 Molecular cloning and characterization of a novel gene, EMILIN-5, and its possible involvement in skeletal development. Biochemical and biophysical research communications 22 14706625
2012 Pooled analyses of the associations of polymorphisms in the GRK4 and EMILIN1 genes with hypertension risk. International journal of medical sciences 21 22639547
2014 Multiple-interactions among EMILIN1 and EMILIN2 N- and C-terminal domains. Matrix biology : journal of the International Society for Matrix Biology 20 25445627
2021 Inactivation of EMILIN-1 by Proteolysis and Secretion in Small Extracellular Vesicles Favors Melanoma Progression and Metastasis. International journal of molecular sciences 19 34299025
2017 The extracellular matrix protein EMILIN1 silences the RAS-ERK pathway via α4β1 integrin and decreases tumor cell growth. Oncotarget 19 28177903
2012 CNGA3 is expressed in inner ear hair cells and binds to an intracellular C-terminus domain of EMILIN1. The Biochemical journal 19 22248097
2009 Emilin1 gene and essential hypertension: a two-stage association study in northern Han Chinese population. BMC medical genetics 17 19922630
1988 Immunochemical characterization of gp115, a yeast glycoprotein modulated by the cell cycle. European journal of cell biology 17 3072201
2020 Distal motor neuropathy associated with novel EMILIN1 mutation. Neurobiology of disease 16 31978608
2017 Fibulin-4 deposition requires EMILIN-1 in the extracellular matrix of osteoblasts. Scientific reports 16 28717224
2006 TGF-beta regulation by Emilin1: new links in the etiology of hypertension. Cell 16 16530037
2022 EMILIN-1 deficiency promotes chronic inflammatory disease through TGFβ signaling alteration and impairment of the gC1q/α4β1 integrin interaction. Matrix biology : journal of the International Society for Matrix Biology 15 35764213
2017 Inhibition of MAPK-Erk pathway in vivo attenuates aortic valve disease processes in Emilin1-deficient mouse model. Physiological reports 15 28270590
2017 The α4β1/EMILIN1 interaction discloses a novel and unique integrin-ligand type of engagement. Matrix biology : journal of the International Society for Matrix Biology 15 29037761
2010 EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro. Connective tissue research 15 20701466
2022 EMILIN1 deficiency causes arterial tortuosity with osteopenia and connects impaired elastogenesis with defective collagen fibrillogenesis. American journal of human genetics 14 36351433
2008 Emilin genes are duplicated and dynamically expressed during zebrafish embryonic development. Developmental dynamics : an official publication of the American Association of Anatomists 14 18095348
2022 Emilin-2 is a component of bone marrow extracellular matrix regulating mesenchymal stem cell differentiation and hematopoietic progenitors. Stem cell research & therapy 13 35012633
2020 Emilin 2 promotes the mechanical gradient of the cochlear basilar membrane and resolution of frequencies in sound. Science advances 13 32577518
2017 Proteomic Alterations Associated with Biomechanical Dysfunction are Early Processes in the Emilin1 Deficient Mouse Model of Aortic Valve Disease. Annals of biomedical engineering 12 28812215
2021 Elastin MIcrofibriL INterfacer1 (EMILIN-1) is an alternative prosurvival VLA-4 ligand in chronic lymphocytic leukemia. Hematological oncology 10 34783040
2020 EMILIN proteins are novel extracellular constituents of the dentin-pulp complex. Scientific reports 10 32948785
2019 Localization, fate and interactions of Emilin-1 in human skin. International journal of cosmetic science 10 30843221
2019 A proteome comparison between human fetal and mature renal extracellular matrix identifies EMILIN1 as a regulator of renal epithelial cell adhesion. Matrix biology plus 10 33543009
2024 Loss of the extracellular matrix glycoprotein EMILIN1 accelerates Δ16HER2-driven breast cancer initiation in mice. NPJ breast cancer 8 38184660
2008 NMR-based homology model for the solution structure of the C-terminal globular domain of EMILIN1. Journal of biomolecular NMR 8 19023665
2025 Dermatofibrosarcoma protuberans with PDGFD rearrangements: a case series featuring a novel EMILIN1::PDGFD fusion and comprehensive literature review. Virchows Archiv : an international journal of pathology 7 40167642
2024 The extracellular matrix protein EMILIN-1 impacts on the microenvironment by hampering gastric cancer development and progression. Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 7 38941035
2022 miR-491-5p inhibits Emilin 1 to promote fibroblasts proliferation and fibrosis in gluteal muscle contracture via TGF-Beta1/Smad2 pathway. Physiological research 7 35275699
2011 Association of intronic single-nucleotide polymorphisms in the EMILIN1 gene with essential hypertension in a Chinese population. Journal of human hypertension 6 21753788
2024 Integrated machine learning-driven disulfidptosis profiling: CYFIP1 and EMILIN1 as therapeutic nodes in neuroblastoma. Journal of cancer research and clinical oncology 5 38427078
2005 Analysis of regulatory regions of Emilin1 gene and their combinatorial contribution to tissue-specific transcription. The Journal of biological chemistry 5 15705587
1990 cAMP promotes the synthesis in early G1 of gp115, a yeast glycoprotein containing glycosyl-phosphatidylinositol. The Journal of biological chemistry 5 2167314
2025 "Unraveling EMILIN-1: A Multifunctional ECM Protein with Tumor-Suppressive Roles" Mechanistic Insights into Cancer Protection Through Signaling Modulation and Lymphangiogenesis Control. Cells 4 40643467
2023 The role of EMILIN-1 in the osteo/odontogenic differentiation of dental pulp stem cells. BMC oral health 4 37024847
2011 [Association of EMILIN1 gene polymorphism with essential hypertension in Mongolian]. Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics 4 21462127
2025 Extravillous trophoblasts reverse the decidualization induced increase in matrix production by secreting TGFβ antagonists Emilin-1 and Gremlin-1. Cells & development 3 39756583
2024 Fe3O4 nanoparticles containing gambogic acid inhibit metastasis in colorectal cancer via the RORB/EMILIN1 axis. Cell adhesion & migration 3 39533963
2025 EMILIN-1 Suppresses Cell Proliferation through Altered Cell Cycle Regulation in Head and Neck Squamous Cell Carcinoma. The American journal of pathology 2 39892781
2025 Behind the scenes: how the EMILIN/Multimerin family shapes the cancer landscape. The FEBS journal 1 41194556
2007 Preparation and characterization of a novel monoclonal antibody specific to human EMILIN-5 protein. Hybridoma (2005) 1 18158787
2026 EMILIN-1 in the tumor microenvironment: insights from CNS tumors and beyond. Frontiers in oncology 0 41994657
2026 EMILIN1 emerges as a TGFβ/SETDB1-regulated secreted biomarker in Duchenne muscular dystrophy. Cell death & disease 0 42106392
2025 Generation of a genome-edited EMILIN1 (c.1606C>T) hiPSC line to investigate aortic aneurysm formation in vitro. Stem cell research 0 40222083
2025 Elastin Microfibril Interface-Located Protein 1 (EMILIN1) Mutation Mimicking Axonal Hereditary Motor Sensory Neuropathy. Cureus 0 40909027

Missed literature

Know a paper Affinage missed for EMILIN1? Flag it for the maintainers and the community.

No submissions yet.