Affinage

EBF2

Transcription factor COE2 · UniProt Q9HAK2

Length
575 aa
Mass
62.6 kDa
Annotated
2026-06-09
40 papers in source corpus 24 papers cited in narrative 24 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

EBF2 is a sequence-specific helix-loop-helix transcription factor that acts as a master determinant of brown and beige adipocyte identity, marking embryonic brown adipogenic precursors and reprogramming myoblasts or white preadipocytes toward a thermogenic fate (PMID:23499423, PMID:25197048, PMID:26844207). Mechanistically, EBF2 recruits PPARγ to brown-fat-selective enhancers and physically engages the BAF chromatin-remodeling complex through BRG1, while transcriptionally activating the brown-selective BAF subunit DPF3 to open chromatin at its target enhancers (PMID:23499423, PMID:28428261); it also cooperates with CBP/P300 to deposit H3K27ac during a defined embryonic window of BAT lineage specification and itself reads the H3K4me1 mark (PMID:39736442, PMID:38015024). EBF2 nucleates phase-separated condensates via a low-complexity C-terminal domain that sequester the repressor ZFP423 and exclude HDAC1 to create a permissive thermogenic chromatin environment, counteracting the antagonistic ZFP423–NuRD corepressor module that otherwise restrains EBF2-bound enhancers in white fat (PMID:42026085, PMID:34620682). Its activity is tuned post-translationally—stabilized against degradation by the deubiquitinase USP2 and activated by Cars2-dependent persulfidation that strengthens its interaction with PPARγ and BRG1—and upstream by SOX4, which is induced by PKA and directly transcribes EBF2 (PMID:40189098, PMID:41849685, PMID:39402212). Beyond adipose tissue, EBF2 directly transactivates the osteoprotegerin (Opg) promoter to regulate osteoclast differentiation and bone mass (PMID:16326388), and is required for GnRH neuron migration, peripheral nerve myelination and Schwann cell differentiation, Cajal-Retzius cell migration, and midbrain PAG dopaminergic neuron development (PMID:12466206, PMID:21220016, PMID:22421355, PMID:25762221). A heterozygous nonsense EBF2 variant (p.E165X) impairs adipocyte differentiation and adipose remodeling and causes glucose intolerance in knock-in mice, linking EBF2 dysfunction to metabolic disease (PMID:41615236).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 2003 High

    Established the first in vivo requirements for EBF2, showing it is essential for neuroendocrine and peripheral nervous system development before any role in fat was known.

    Evidence Targeted Ebf2 deletion in mice with neuroanatomy and nerve electrophysiology

    PMID:12466206

    Open questions at the time
    • Direct transcriptional targets in GnRH neurons and nerve not identified
    • Cell-autonomous vs non-autonomous mechanism unresolved
  2. 2005 High

    Identified the first direct EBF2 target gene, Opg, defining a transcriptional mechanism linking EBF2 to bone homeostasis via osteoclast control.

    Evidence Promoter binding/transactivation assays and Ebf2 KO mice with bone phenotype

    PMID:16326388

    Open questions at the time
    • Cofactors at the Opg promoter beyond LEF1/TCF not fully defined
    • Direct vs indirect contribution to bone mass not dissected
  3. 2006 Medium

    Placed EBF proteins within the core adipogenic cascade by showing they directly activate PPARγ1 and C/EBPα, before brown-specific roles were known.

    Evidence Promoter activation assays and shRNA knockdown in 3T3-L1 cells

    PMID:17060461

    Open questions at the time
    • EBF1 vs EBF2 specific contributions not separated
    • Single lab
  4. 2013 High

    Revealed the defining function of EBF2 as a brown-fat determinant that recruits PPARγ to brown-selective enhancers, explaining how a shared adipogenic factor specifies lineage identity.

    Evidence ChIP-seq, ectopic expression in myoblasts/white preadipocytes, Ebf2 KO mice

    PMID:23499423

    Open questions at the time
    • Chromatin-remodeling machinery not yet identified
    • How brown-specific enhancer selection is achieved unresolved
  5. 2014 High

    Defined EBF2 as a prospective marker and regulator of embryonic brown adipogenic precursors, distinguishing brown vs beige fates by cellular origin.

    Evidence Ebf2-GFP reporter mice, prospective cell isolation, RNA-seq, KO and ectopic expression

    PMID:25197048

    Open questions at the time
    • Molecular basis of brown vs beige fate divergence not defined
  6. 2015 High

    Extended EBF2's requirement to inducible beige fat, showing it is necessary and sufficient for the adrenergically driven thermogenic program in WAT.

    Evidence Ebf2 KO and adipose-transgenic OE mice, primary cultures, UCP1/mitochondrial assays

    PMID:26844207

    Open questions at the time
    • Connection between adrenergic signaling and EBF2 activity not mechanistically traced
  7. 2017 High

    Provided the chromatin-remodeling mechanism for EBF2 function, showing it recruits BAF via BRG1 and activates the brown-selective subunit DPF3 to open its target enhancers.

    Evidence Reciprocal Co-IP, ChIP-seq, ATAC-seq with DPF3 loss-of-function, promoter assays

    PMID:28428261

    Open questions at the time
    • Structural basis of EBF2–BRG1 interaction unknown
    • How DPF3 enforces brown selectivity not resolved
  8. 2021 High

    Defined the molecular antagonism restraining EBF2 in white fat, showing ZFP423 recruits NuRD to EBF2 enhancers and that breaking this interaction triggers a corepressor-to-coactivator switch and browning.

    Evidence Co-IP, CRISPR disruption of the PPI, PPARγ ChIP-seq, Zfp423 conditional KO

    PMID:34620682

    Open questions at the time
    • Signals controlling the ZFP423–EBF2 interaction in vivo not identified
  9. 2024 Medium

    Identified upstream transcriptional and signaling control of EBF2 by SOX4, linking PKA signaling to EBF2 induction and thermogenesis.

    Evidence Sox4 conditional KO and OE, ChIP at the EBF2 promoter, Co-IP, PKA phosphorylation assays

    PMID:39402212 PMID:40977422

    Open questions at the time
    • Whether SOX4 and EBF2 functions are separable in vivo not fully resolved
    • Single lab
  10. 2024 Medium

    Showed EBF2 reads H3K4me1 and cooperates with KMT2D to activate KLLN, extending EBF2's coactivator partnerships and revealing a tumor-suppressive role in PDAC.

    Evidence ChIP-seq, RNA-seq, co-occupancy, functional proliferation/invasion assays

    PMID:38015024

    Open questions at the time
    • Generality of H3K4me1 reading across EBF2 target loci untested
    • Single lab
  11. 2024 Medium

    Connected EBF2 to histone acetylation by showing it recruits CBP/P300 to deposit H3K27ac during a critical embryonic window of BAT specification.

    Evidence ChIP-seq histone mark dynamics, Co-IP, ectopic expression, comparative species analysis

    PMID:39736442

    Open questions at the time
    • Temporal coordination with BAF/DPF3 remodeling not integrated
    • Single lab
  12. 2025 Medium

    Revealed post-translational stabilization of EBF2 by the deubiquitinase USP2 as a control point for thermogenic capacity and obesity resistance.

    Evidence Co-IP, proteome-wide substrate ID, AAV/lentiviral KD/OE in vivo, metabolic phenotyping

    PMID:40189098

    Open questions at the time
    • E3 ligase opposing USP2 not identified
    • Single lab
  13. 2025 Medium

    Linked EBF2 directly to mitochondrial phospholipid biology, showing it binds Srebf1 and controls cardiolipin/PE synthesis and fission-fusion machinery in brown adipocytes.

    Evidence Myf5Cre Ebf2 KO, lipidomics, ChIP at Srebf1, electron microscopy

    PMID:40865612

    Open questions at the time
    • Whether mitochondrial defects are direct or secondary to lost identity unclear
    • Single lab
  14. 2026 Medium

    Demonstrated that EBF2 forms phase-separated condensates that sequester ZFP423 and exclude HDAC1, providing a biophysical mechanism for creating a permissive thermogenic chromatin environment.

    Evidence ΔCTD/proline mutants in vitro and in mice, live-cell imaging, Co-IP, ChIP, IDR swaps

    PMID:42026085

    Open questions at the time
    • Endogenous condensate dynamics in vivo not fully quantified
    • Single lab
  15. 2026 Medium

    Identified Cars2-mediated persulfidation as a post-translational activation switch enhancing EBF2 interaction with PPARγ and BRG1, with EBF2 also transcribing Cars2 in a feedforward loop.

    Evidence Co-IP after persulfidation, ChIP, Cars2 KO, H2S donor and persulfidation biochemistry

    PMID:41849685

    Open questions at the time
    • Persulfidated residues on EBF2 not mapped
    • Single lab
  16. 2026 High

    Established a disease link by showing a heterozygous EBF2 nonsense variant impairs adipose differentiation and remodeling and causes metabolic dysfunction.

    Evidence Ebf2E165X/+ knock-in mice, in vitro adipogenesis, metabolic phenotyping, histology

    PMID:41615236

    Open questions at the time
    • Human patient genetics beyond the modeled variant not detailed
    • Mechanism of dominant effect not fully dissected

Open questions

Synthesis pass · forward-looking unresolved questions
  • How EBF2's many post-translational, condensate, and cofactor inputs are integrated to achieve tissue-specific target selection, and how its neuronal/skeletal roles relate mechanistically to its adipose function, remain open.
  • No structural model of EBF2–PPARγ or EBF2–BRG1 complexes
  • Direct targets in neurons largely unidentified
  • Integration of persulfidation, ubiquitination, and condensate formation not unified

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140110 transcription regulator activity 6 GO:0003677 DNA binding 3 GO:0042393 histone binding 1 GO:0140313 molecular sequestering activity 1
Localization
GO:0005634 nucleus 3
Pathway
R-HSA-1266738 Developmental Biology 4 R-HSA-1430728 Metabolism 3 R-HSA-4839726 Chromatin organization 3 R-HSA-74160 Gene expression (Transcription) 3
Partners
BRG1CBP/P300DPF3HDAC1PPARGSOX4USP2ZFP423
Complex memberships
BAF chromatin remodeling complex

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2013 EBF2 recruits PPARγ to brown adipose-specific binding sites to determine brown versus white adipocyte identity; the EBF DNA-binding motif is highly enriched within brown adipose-specific PPARγ binding sites identified by ChIP-seq, and expression of EBF2 in myoblasts or white preadipose cells reprograms cells to a brown fat fate. ChIP-seq, ectopic expression in myoblasts/white preadipose cells, Ebf2 knockout mice Cell metabolism High 23499423
2017 EBF2 physically interacts with the BAF chromatin remodeling complex (via BRG1) and recruits it to lineage-specific enhancers in brown adipocytes; EBF2 directly transcriptionally activates DPF3, a brown fat-selective BAF subunit, which in turn is required for chromatin accessibility at EBF2-bound enhancers and for brown fat gene programming. ChIP-seq, co-immunoprecipitation (EBF2–BRG1 interaction), DPF3 loss-of-function (ATAC-seq/chromatin accessibility), direct promoter activation assays Genes & development High 28428261
2021 ZFP423 physically interacts with EBF2 and recruits the NuRD corepressor complex to EBF2-bound thermogenic gene enhancers in white adipocytes; disruption of the ZFP423–EBF2 protein–protein interaction via CRISPR-Cas9 editing triggers an EBF2 NuRD-to-BAF coregulator switch, shifts PPARγ occupancy toward thermogenic genes, and induces widespread browning of WAT. Co-IP, CRISPR-Cas9 disruption of protein–protein interaction, ChIP-seq (PPARγ occupancy), Zfp423 conditional KO Genes & development High 34620682
2005 EBF2 binds to sequences in the Opg (osteoprotegerin) promoter and transactivates it in synergy with the Wnt-responsive LEF1/TCF:β-catenin pathway; loss of Ebf2 in mice causes downregulation of OPG, leading to increased osteoclast numbers and reduced bone mass. Promoter binding/transactivation assays, Ebf2 targeted knockout mice, osteoclast phenotype analysis Developmental cell High 16326388
2006 EBF1 and EBF2 promote adipogenesis by directly activating the PPARγ1 and C/EBPα promoters; EBF1 is itself induced by C/EBPβ/δ; knockdown of Ebf1/Ebf2 by shRNA blocks 3T3-L1 differentiation, placing EBF proteins within the core adipogenic transcriptional cascade. Promoter activation assays (direct binding), shRNA knockdown in 3T3-L1 cells, ectopic expression in NIH 3T3 fibroblasts Molecular and cellular biology Medium 17060461
2003 Loss of Ebf2 in mice causes defective migration of GnRH-synthesizing neurons, impaired formation of the neuroendocrine axis, and hypogonadotropic hypogonadism; Ebf2-null peripheral nerves show axon-sorting defects, hypomyelination, and severely reduced motor nerve conduction velocity, establishing EBF2 as required for GnRH neuron migration and peripheral nerve development. Targeted Ebf2 gene deletion in mice, histological/neuroanatomical analysis, electrophysiology (motor nerve conduction velocity) Development (Cambridge, England) High 12466206
2014 Ebf2 marks and regulates embryonic brown adipogenic precursor cells; Ebf2-expressing cells from brown fat tissue uniformly differentiate into brown adipocytes, those from white fat into beige adipocytes; loss of Ebf2 reduces brown preadipose-signature genes while ectopic Ebf2 expression in myoblasts activates brown preadipose-specific genes. Ebf2-GFP reporter mouse, prospective cell isolation, RNA-seq, loss-of-function (Ebf2 KO), ectopic expression in myoblasts Proceedings of the National Academy of Sciences of the United States of America High 25197048
2015 EBF2 is required for beige adipocyte development; Ebf2 knockout mice fail to induce UCP1 or a thermogenic program in subcutaneous WAT following adrenergic stimulation, and EBF2 overexpression in adipocyte cultures or transgenic adipose tissue is sufficient to induce UCP1 expression, brown-like differentiation, and increased mitochondrial function. Ebf2 KO mice, transgenic Ebf2 overexpression in adipose tissue, primary adipose cell cultures, adrenergic stimulation, UCP1 and mitochondrial function assays Molecular metabolism High 26844207
2009 EBF2 functions as a transcriptional activator of OPG in osteosarcoma; knockdown of EBF2 reduces OPG levels and increases sensitivity to TRAIL-induced apoptosis, demonstrating that EBF2-driven OPG expression contributes to TRAIL resistance in osteosarcoma. Lentiviral shRNA knockdown of EBF2, real-time PCR, ELISA (OPG protein), caspase-3/7 apoptosis assay Clinical cancer research Medium 19671856
2011 Ebf2 is expressed in Schwann cells; loss of Ebf2 delays the onset of myelination, downregulates Schwann cell differentiation markers, causes nodal region abnormalities, shorter internodal length, and impairs motor nerve conduction velocity and amplitude, establishing EBF2 as required for peripheral nerve myelination and Schwann cell differentiation. Ebf2 KO mice, immunohistochemistry, electrophysiology (nerve conduction), morphometric analysis Neurobiology of disease High 21220016
2016 EBF1 and EBF2 act as positive regulators of myelination in Schwann cells; combined knockdown of Ebf genes in myelinating dorsal root ganglia cultures severely impairs myelin formation, rescued by specific overexpression; EBF target gene profiling identifies Gliomedin as a direct target regulated by EBF factors in peripheral myelination. siRNA/shRNA knockdown and overexpression in DRG myelinating cultures, EBF target gene profiling, promoter regulation assays for Gliomedin Molecular neurobiology Medium 27889898
2012 Loss of Ebf2 causes a transient decrease in Cajal-Retzius cell numbers on the cortical surface due to a migratory defect originating from the cortical hem; both EBF2 and EBF3 directly control CR cell migration in vitro. Ebf2 KO mice, fate-mapping studies, in vitro cortical hem preparations with migration assays Developmental biology Medium 22421355
2011 Ebf2 overexpression increases the generation of early-born neurons including Cajal-Retzius cells, while Ebf2 knockdown decreases it, demonstrating a direct regulatory role for EBF2 in cortical neurogenesis and CR neuron generation without affecting other layer-specific neurons. Lentiviral-mediated knockdown and overexpression in cortical cultures, Ebf2-EGFP transgenic reporter line Developmental neuroscience Medium 22042145
2015 Loss of Ebf2 in vivo causes a marked reduction in dopaminergic neuron number specifically in the midbrain periaqueductal gray matter but not in the substantia nigra or ventral tegmental area, demonstrating a selective requirement for EBF2 in PAG DA neuron development. Ebf2 KO mice, immunohistochemistry, quantification of TH+ neurons across midbrain subregions Developmental neurobiology Medium 25762221
2023 EBF2 binds H3K4me1 (monomethylated histone H3 Lys4) and cooperates with KMT2D (which catalyzes H3K4me1) at the KLLN gene promoter region to activate KLLN transcription; KMT2D and EBF2 cooperatively suppress PDAC cell proliferation, migration, and invasion through KLLN upregulation. ChIP-seq, RNA-seq, co-occupancy analysis, functional assays (proliferation, migration, invasion), EBF2 identified as H3K4me1-binding protein Advanced science Medium 38015024
2024 SOX4 activates EBF2 transcription by directly binding its promoter; SOX4 and EBF2 cooperate to activate thermogenic gene expression in brown adipose tissue; phosphorylation of SOX4 at Ser235 by PKA promotes its nuclear translocation and thereby enhances EBF2 transcription. Sox4 conditional KO mice, SOX4 overexpression in BAT, ChIP assays (SOX4 binding to EBF2 promoter), co-immunoprecipitation (SOX4–EBF2 complex), PKA phosphorylation assays Cell death and differentiation Medium 39402212
2025 The deubiquitinating enzyme USP2 physically interacts with EBF2 and stabilizes it by removing ubiquitin, thereby preventing EBF2 degradation; BAT-specific Usp2 knockdown impairs thermogenic programs, while Usp2 overexpression in BAT protects against obesity; EBF2 was identified as the substrate of USP2 mediating its thermogenic function. Co-immunoprecipitation, quantitative proteomics (substrate identification), adeno-associated virus and lentivirus KD/OE in vivo and in vitro, metabolic phenotyping Molecular metabolism Medium 40189098
2025 EBF2 directly regulates mitochondrial membrane phospholipid composition in brown adipocytes; Myf5Cre-driven Ebf2 deletion drastically reduces cardiolipin and phosphatidylethanolamine abundance and alters acyl chain remodeling; EBF2 directly binds the Srebf1 promoter and regulates expression of cardiolipin/PE-synthesizing genes; Ebf2 deletion reduces DRP1 and OPA1 levels, impairing mitochondrial fission-fusion dynamics. Myf5Cre conditional Ebf2 KO mice, lipidomics, ChIP (EBF2 binding to Srebf1), gene expression analysis, electron microscopy Journal of lipid research Medium 40865612
2026 Cars2-generated cysteine persulfide post-translationally modifies EBF2 via persulfidation; persulfidated EBF2 shows enhanced interaction with PPARγ and BRG1, increasing recruitment of the EBF2–PPARγ complex to browning gene promoters to drive brown fat development and thermogenesis; Cars2 is itself a direct transcriptional target of EBF2. Co-IP (EBF2–PPARγ and EBF2–BRG1 interactions after persulfidation), ChIP (EBF2-PPARγ complex at browning gene promoters), Cars2 KO in thermogenic fat, H2S donor treatment, EBF2 persulfidation biochemical assays Advanced science Medium 41849685
2026 EBF2 contains a low-complexity C-terminal domain (CTD) that drives biomolecular condensate (phase separation) formation; deletion of the CTD or mutation of conserved proline residues disrupts EBF2 phase separation without affecting genomic occupancy; EBF2 condensates sequester the transcriptional repressor ZFP423 and exclude HDAC1, creating a permissive chromatin environment for thermogenic gene expression; fusion of an IDR from FUS but not MED1 rescues condensate function. EBF2 ΔCTD/proline mutants in vitro and in male mice, live-cell condensate imaging, co-immunoprecipitation (ZFP423/HDAC1 in condensates), ChIP, small-molecule condensate screen Nature communications Medium 42026085
2026 A heterozygous nonsense EBF2 variant (p.E165X) impairs adipocyte differentiation and adipose tissue remodeling in vitro and in Ebf2E165X/+ knock-in mice; the variant causes excess accumulation of undifferentiated CD34+ cells, defective extracellular matrix remodeling, abnormal adipocyte hypertrophy, reduced adiponectin/leptin expression, glucose intolerance, and downregulation of mitochondrial fatty acid metabolism genes specifically in adipose tissue. Heterozygous knock-in (Ebf2E165X/+) mice, in vitro adipogenesis assays, gene expression analysis, metabolic phenotyping, histology The Journal of clinical investigation High 41615236
2024 EBF2 interacts with transcriptional co-activators CBP/P300 to induce H3K27ac deposition and chromatin activation at brown adipose tissue-associated genes, driving progressive specification of the brown fat lineage from Pax7+ mesodermal stem cells; EBF2 expression during a critical embryonic window (E10.5–E14.5 in mouse) is required for BAT lineage specification. ChIP-seq (H3K4me3, H3K27me3, H3K27ac dynamics), Co-IP (EBF2–CBP/P300 interaction), comparative mouse/pig analysis, ectopic EBF2 expression in mesodermal stem cells Journal of advanced research Medium 39736442
2025 SOX4 forms two independent complexes with EBF2 and with PPARγ to promote thermogenic gene expression in BAT; SOX4 directly binds the promoter regions of thermogenic genes independent of EBF2; deletion of SOX4 in BAT (Sox4-BKO mice) downregulates thermogenic and oxidative phosphorylation genes and reduces mitochondrial numbers. Co-immunoprecipitation (SOX4–EBF2 and SOX4–PPARγ complexes), ChIP (SOX4 direct binding to thermogenic gene promoters), Sox4 conditional KO (Ucp1Cre+), in vitro loss-of-function Advanced biology Medium 40977422
2018 miR-204-5p directly binds the 3' UTR of EBF2 mRNA to regulate its stability; overexpression of EBF2 inhibits apoptosis and promotes migration and invasion of osteosarcoma cells, and EBF2 overexpression rescues the pro-apoptotic/anti-migratory phenotype caused by miR-204-5p overexpression. Luciferase reporter assay (miR-204-5p binding to EBF2 3'UTR), EBF2 overexpression rescue experiments, apoptosis assays, migration/invasion assays, xenograft model Biochimie Medium 30529043

Source papers

Stage 0 corpus · 40 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2003 Plant responses to ethylene gas are mediated by SCF(EBF1/EBF2)-dependent proteolysis of EIN3 transcription factor. Cell 636 14675532
2013 EBF2 determines and maintains brown adipocyte identity. Cell metabolism 307 23499423
2014 Ebf2 is a selective marker of brown and beige adipogenic precursor cells. Proceedings of the National Academy of Sciences of the United States of America 195 25197048
2006 Critical role for Ebf1 and Ebf2 in the adipogenic transcriptional cascade. Molecular and cellular biology 183 17060461
2015 Seedlings Transduce the Depth and Mechanical Pressure of Covering Soil Using COP1 and Ethylene to Regulate EBF1/EBF2 for Soil Emergence. Current biology : CB 122 26748855
2005 EBF2 regulates osteoblast-dependent differentiation of osteoclasts. Developmental cell 96 16326388
2015 EBF2 promotes the recruitment of beige adipocytes in white adipose tissue. Molecular metabolism 91 26844207
2003 Hypogonadotropic hypogonadism and peripheral neuropathy in Ebf2-null mice. Development (Cambridge, England) 88 12466206
2017 EBF2 transcriptionally regulates brown adipogenesis via the histone reader DPF3 and the BAF chromatin remodeling complex. Genes & development 69 28428261
2009 Silencing Sl-EBF1 and Sl-EBF2 expression causes constitutive ethylene response phenotype, accelerated plant senescence, and fruit ripening in tomato. Journal of experimental botany 69 19903730
1998 Molecular cloning of Zcoe2, the zebrafish homolog of Xenopus Xcoe2 and mouse EBF-2, and its expression during primary neurogenesis. Mechanisms of development 61 9784615
2004 Her5 acts as a prepattern factor that blocks neurogenin1 and coe2 expression upstream of Notch to inhibit neurogenesis at the midbrain-hindbrain boundary. Development (Cambridge, England) 59 15056616
2018 MiR-204-5p promotes apoptosis and inhibits migration of osteosarcoma via targeting EBF2. Biochimie 39 30529043
2012 Early B-cell factors 2 and 3 (EBF2/3) regulate early migration of Cajal-Retzius cells from the cortical hem. Developmental biology 37 22421355
2005 Molecular analysis of KAL-1, GnRH-R, NELF and EBF2 genes in a series of Kallmann syndrome and normosmic hypogonadotropic hypogonadism patients. The Journal of endocrinology 36 16423815
2009 Profiling of chemonaive osteosarcoma and paired-normal cells identifies EBF2 as a mediator of osteoprotegerin inhibition to tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis. Clinical cancer research : an official journal of the American Association for Cancer Research 34 19671856
2021 ZFP423 controls EBF2 coactivator recruitment and PPARγ occupancy to determine the thermogenic plasticity of adipocytes. Genes & development 29 34620682
2023 Inhibition of pathologic immunoglobulin E in food allergy by EBF-2 and active compound berberine associated with immunometabolism regulation. Frontiers in immunology 15 36825019
2023 EBF2 Links KMT2D-Mediated H3K4me1 to Suppress Pancreatic Cancer Progression via Upregulating KLLN. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 15 38015024
2011 Both Schwann cell and axonal defects cause motor peripheral neuropathy in Ebf2-/- mice. Neurobiology of disease 15 21220016
2012 Motor dysfunction and cerebellar Purkinje cell firing impairment in Ebf2 null mice. Molecular and cellular neurosciences 14 23000673
2019 Potential Regulatory Effects of miR-182-3p in Osteosarcoma via Targeting EBF2. BioMed research international 12 30993113
2016 The Transcription Factors EBF1 and EBF2 Are Positive Regulators of Myelination in Schwann Cells. Molecular neurobiology 11 27889898
2024 SOX4 facilitates brown fat development and maintenance through EBF2-mediated thermogenic gene program in mice. Cell death and differentiation 10 39402212
2024 Elevated EBF2 in mouse but not pig drives the progressive brown fat lineage specification via chromatin activation. Journal of advanced research 9 39736442
2022 COE2 Is Required for the Root Foraging Response to Nitrogen Limitation. International journal of molecular sciences 9 35055047
2011 Ebf2 marks early cortical neurogenesis and regulates the generation of cajal-retzius neurons in the developing cerebral cortex. Developmental neuroscience 9 22042145
2015 Ebf2 is required for development of dopamine neurons in the midbrain periaqueductal gray matter of mouse. Developmental neurobiology 8 25762221
2019 Morphological and Physiological Characteristics of Ebf2-EGFP-Expressing Cajal-Retzius Cells in Developing Mouse Neocortex. Cerebral cortex (New York, N.Y. : 1991) 6 30307495
2018 Variants in the Gene EBF2 Are Associated with Kawasaki Disease in a Korean Population. Yonsei medical journal 5 29749135
2022 Age-Progressive and Gender-Dependent Bone Phenotype in Mice Lacking Both Ebf1 and Ebf2 in Prrx1-Expressing Mesenchymal Cells. Calcified tissue international 4 35137272
2017 Pioneering EBF2 remodels the brown fat chromatin landscape. Genes & development 4 28446594
2018 A missense mutation in EBF2 was segregated with imperforate anus in a family across three generations. American journal of medical genetics. Part A 3 29704291
2025 SOX4 Regulates Thermogenesis in Brown Adipose Tissue via Independent Complexes with EBF2 and PPARγ. Advanced biology 2 40977422
2025 Deubiquitinating enzyme USP2 regulates brown adipose tissue thermogenesis via controlling EBF2 stabilization. Molecular metabolism 1 40189098
2026 EBF2 variant identified in a patient with atypical partial lipodystrophy causes adipose fibrosis and dysfunction. The Journal of clinical investigation 0 41615236
2026 Cars2-Mediated Cysteine Catabolism Drives Brown Fat Development and Thermogenesis Through Persulfidating EBF2. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 0 41849685
2026 Postnatal expression of the transcription factor Ebf2 in motivation, reward, and pain-related circuits of the mouse brain. Frontiers in neuroanatomy 0 41859334
2026 EBF2 condensates enable adipose thermogenesis through ZFP423 sequestration and epigenetic remodeling. Nature communications 0 42026085
2025 EBF2 regulates cardiolipin and phosphatidylethanolamine remodeling and mitochondrial dynamics in brown fat. Journal of lipid research 0 40865612

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