Affinage

CSRP3

Cysteine and glycine-rich protein 3 · UniProt P50461

Length
194 aa
Mass
21.0 kDa
Annotated
2026-04-28
100 papers in source corpus 14 papers cited in narrative 14 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CSRP3 (MLP) is a LIM-domain protein essential for striated muscle cytoarchitecture, mechanotransduction, and cardiomyocyte survival. At the cytoskeleton, CSRP3 links actin-based structures to the sarcolemma by binding βI-spectrin at costameres and α-actinin at Z-lines, and its loss causes dilated cardiomyopathy with disrupted myofibrillar organization and dysregulated calcium homeostasis that can be rescued by calcium channel blockade (PMID:9039266, PMID:10751147, PMID:31406109). CSRP3 undergoes syndecan-4-dependent nuclear translocation where it interacts with myogenic transcription factors MyoD and MyoG to promote muscle differentiation, and in the cytoplasm it directly binds LC3 to support autophagosome formation, with its silencing impairing autophagic flux and increasing apoptosis (PMID:30967474, PMID:35652451, PMID:27551448). Missense mutations in CSRP3 cause hypertrophic cardiomyopathy through protein destabilization and disrupted interactions, while S-nitrosylation at Cys79 in hypertrophic myocardium drives pathological signaling by recruiting TLR3 to activate the RIP3–NLRP3 inflammasome pathway (PMID:18505755, PMID:20044516, PMID:31902237).

Mechanistic history

Synthesis pass · year-by-year structured walk · 10 steps
  1. 1997 High

    Establishing that CSRP3 is required for cardiomyocyte cytoarchitecture answered the fundamental question of whether this LIM-domain protein has a structural role in terminally differentiated muscle, rather than being purely transcriptional.

    Evidence MLP knockout mice developing dilated cardiomyopathy with disrupted actin-based structures, ultrastructural and cardiac phenotyping

    PMID:9039266

    Open questions at the time
    • Specific molecular partners at the cytoskeleton not yet identified
    • Whether the phenotype reflects a structural versus signaling defect was unresolved
  2. 2000 High

    Identifying βI-spectrin as a direct binding partner of the second LIM domain and demonstrating co-localization at costameres established CSRP3 as a physical linker between the spectrin membrane skeleton and the sarcomeric actin cytoskeleton.

    Evidence Yeast two-hybrid, in vitro binding assays, confocal co-localization at sarcolemma overlying Z- and M-lines

    PMID:10751147

    Open questions at the time
    • Whether CSRP3 bridges spectrin and α-actinin simultaneously was not tested
    • Role at premyofibril versus mature costamere stages was unclear
  3. 2003 Medium

    Tracking CSRP3 localization during myofibrillogenesis revealed it acts at premyofibril and nascent myofibril stages before costamere formation, distinguishing its temporal role from that of the related PDZ-LIM protein ALP.

    Evidence Immunofluorescence during cardiomyocyte differentiation in embryonic chick cultures

    PMID:12589684

    Open questions at the time
    • Whether premyofibril localization is functionally required was not tested by perturbation
    • Mammalian validation not performed
  4. 2008 High

    Demonstrating that CSRP3 missense mutations segregate with hypertrophic cardiomyopathy and that mutant protein is destabilized in patient hearts established CSRP3 as a bona fide HCM disease gene and showed that its predominant localization is cytosolic rather than tightly sarcomere-anchored.

    Evidence Linkage analysis in multiple families, monoclonal antibody immunolocalization, mutant protein stability assays

    PMID:18505755

    Open questions at the time
    • Mechanism by which destabilization leads to hypertrophy not defined
    • Whether all HCM mutations act through destabilization versus altered interactions was unknown
  5. 2009 High

    The W4R knock-in mouse showed that a specific HCM-linked mutation weakens telethonin binding and increases nuclear MLP accumulation, establishing that disease mutations can alter both protein–protein interactions and subcellular distribution in a dosage- and age-dependent manner.

    Evidence Knock-in mice, in vitro telethonin–MLP binding assay, immunohistochemistry, Western blot

    PMID:20044516

    Open questions at the time
    • Whether increased nuclear MLP is pathogenic or compensatory was unclear
    • The nuclear function of MLP remained undefined
  6. 2015 Medium

    Discovery that CSRP3 directly binds LC3 and is required for proper autophagosome formation revealed a previously unsuspected role in muscle autophagy, linking its loss to impaired protein turnover and increased apoptotic vulnerability.

    Evidence Co-immunoprecipitation, proximity ligation assay, siRNA knockdown with autophagic flux measurements

    PMID:27551448

    Open questions at the time
    • The LC3-binding domain on CSRP3 was not mapped
    • Whether autophagy impairment contributes to the cardiomyopathy phenotype in vivo was untested
  7. 2019 High

    Identification of syndecan-4 as a direct CSRP3 interactor that mediates its nuclear translocation resolved the long-standing question of how a predominantly cytoskeletal protein accesses the nucleus, and showed this interaction is enhanced in heart failure.

    Evidence Affinity purification-MS, reciprocal co-IP, nuclear fractionation in syndecan-4 KO/overexpression models, disruptor peptide

    PMID:30967474

    Open questions at the time
    • Nuclear targets of translocated MLP were not identified in this study
    • Whether syndecan-4–MLP disruption is therapeutic in heart failure models was not shown
  8. 2019 High

    CRISPR knockout of CSRP3 in human cardiomyocytes showed that elevated intracellular calcium is a central consequence of MLP loss, and pharmacological calcium normalization prevents HCM-like progression to heart failure, establishing calcium dysregulation as a unifying downstream mechanism.

    Evidence CSRP3 CRISPR KO in hESC-derived cardiomyocytes, calcium imaging, verapamil rescue

    PMID:31406109

    Open questions at the time
    • The molecular basis of calcium elevation (channel, SERCA, leak) was not defined
    • Whether calcium normalization rescues in vivo models was untested
  9. 2020 High

    Identification of S-nitrosylation at Cys79 as a post-translational modification that converts CSRP3 into a pro-hypertrophic signaling molecule—by enabling TLR3 binding and RIP3–NLRP3 inflammasome activation—revealed a gain-of-function inflammatory mechanism distinct from loss-of-function structural roles.

    Evidence Biotin-switch assay, LC-MS/MS SNO-site mapping, C79 mutagenesis, TLR3 co-IP and KO mouse, transverse aortic constriction model

    PMID:31902237

    Open questions at the time
    • Whether SNO-MLP-TLR3 axis operates in human heart failure tissue was not shown
    • The nitrosylase/denitrosylase balance controlling Cys79 modification in vivo is unclear
  10. 2022 Medium

    Demonstrating that nuclear CSRP3 directly interacts with MyoD and MyoG identified transcription-factor partners and provided a molecular function for nuclear MLP in promoting myogenic differentiation and repair.

    Evidence Co-immunoprecipitation in C2C12 cells, nuclear translocation assays, mouse muscle injury model

    PMID:35652451

    Open questions at the time
    • Whether CSRP3 acts as a co-activator or chromatin remodeler at myogenic promoters is unknown
    • Genome-wide binding sites of CSRP3 have not been determined

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the cytoskeletal scaffolding, autophagy-promoting, and nuclear transcriptional co-regulatory functions of CSRP3 are coordinated in response to mechanical load, and which function is primarily disrupted in each cardiomyopathy-causing mutation, remains unresolved.
  • No structural model of CSRP3 LIM domains bound to any partner exists
  • The molecular basis of calcium dysregulation upon CSRP3 loss is not defined
  • Whether autophagy impairment contributes to in vivo cardiomyopathy phenotypes is untested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 4 GO:0060090 molecular adaptor activity 3
Localization
GO:0005856 cytoskeleton 4 GO:0005634 nucleus 3 GO:0005829 cytosol 2 GO:0005886 plasma membrane 1
Pathway
R-HSA-1643685 Disease 4 R-HSA-1266738 Developmental Biology 2 R-HSA-5357801 Programmed Cell Death 2 R-HSA-9612973 Autophagy 2 R-HSA-168256 Immune System 1
Partners
ACTN2MAP1LC3BMYOD1MYOGSDC4SPTBN1TCAPTLR3

Evidence

Reading pass · 14 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 MLP (CSRP3) knockout mice develop dilated cardiomyopathy with disruption of cardiomyocyte cytoarchitecture, establishing that MLP promotes proper cardiomyocyte cytoarchitecture organization at actin-based structures in terminally differentiated striated muscle cells. Gene knockout in mice (MLP-deficient), ultrastructural analysis, in vivo cardiac phenotyping Cell High 9039266
2000 MLP (CSRP3) interacts with betaI-spectrin via its second LIM motif (binding to repeat 7 of beta-spectrin), and the two proteins co-localize at the sarcolemma overlying Z- and M-lines of myofibrils in cardiac and skeletal muscle, suggesting MLP functions as a costamere protein linking the beta-spectrin network to alpha-actinin crosslinked actin filaments. Yeast two-hybrid screen, in vitro and in vivo protein interaction assays, confocal microscopy co-localization Journal of cell science High 10751147
2000 MLP protein is present in the contractile protein fraction of cardiomyocytes, and its binding to myofibrils requires functional zinc finger (LIM) domains; MLP protein (but not mRNA) is decreased ~50% in failing human hearts, suggesting post-translational regulation. Western blot fractionation, domain mutagenesis, Northern blot, immunohistochemistry Circulation Medium 10851202
2003 MLP and ALP (a related PDZ-LIM protein) distribute to distinct subcellular compartments during myofibrillogenesis in cardiomyocytes: ALP co-distributes with alpha-actinin to Z-lines from the earliest stages, while MLP localizes to premyofibrils and nascent myofibrils before costamere establishment, suggesting MLP functions during differentiation prior to costamere formation. Immunofluorescence microscopy during myofibrillogenesis in cultured embryonic chick cardiomyocytes, GFP transfection, in vitro binding assay Cell motility and the cytoskeleton Medium 12589684
2008 CSRP3 missense mutations cause hypertrophic cardiomyopathy (HCM); MLP is mainly a cytosolic component of cardiomyocytes (not tightly anchored to sarcomeric structures); at least one HCM-associated mutant form of MLP is destabilized in patient hearts. Linkage analysis, monoclonal antibody-based immunolocalization, in vitro and in vivo functional analysis of mutant MLP stability Human molecular genetics High 18505755
2009 The W4R (Trp4Arg) MLP variant causes reduced MLP mRNA and protein expression, weaker in vitro interaction of telethonin with W4R-MLP compared to wild-type MLP, and increased nuclear localization of W4R-MLP; knock-in mice develop age- and gene dosage-dependent hypertrophic cardiomyopathy. Knock-in mouse generation, in vitro binding assay (telethonin-MLP interaction), immunohistochemistry, Western blot Circulation research High 20044516
2009 CRP3/MLP expression in vascular smooth muscle cells is induced by mechanical stretch (not shear stress) during vein arterialization, identifying stretch as the primary biomechanical stimulus for CSRP3 induction in this context. Ex vivo flow-through system mimicking arterial conditions, in vivo rat vein arterialization model, quantitative RT-PCR, immunostaining Cardiovascular research Medium 19351738
2015 MLP (CSRP3) contributes to correct autophagosome formation and flux in muscle cells by directly interacting with LC3, as demonstrated by co-immunoprecipitation and proximity ligation assay; MLP silencing results in decreased LC3-II, impaired degradation of long-lived proteins, and increased apoptotic susceptibility. Co-immunoprecipitation, proximity ligation assay (PLA), siRNA knockdown, Western blot, ultrastructural analysis Cell death discovery Medium 27551448
2019 Syndecan-4 directly binds MLP (CSRP3) with increased binding in heart failure; syndecan-4 mediates nuclear translocation of MLP in cardiomyocytes, as loss of syndecan-4 reduces nuclear MLP and overexpression increases it; a cell-permeable syndecan-4–MLP disruptor peptide reduces nuclear MLP levels. Affinity purification-MS interactome mapping, co-immunoprecipitation validation in HEK293 cells, nuclear fractionation of syndecan-4 knockout and overexpression models, disruptor peptide experiment The Journal of biological chemistry High 30967474
2019 MLP-deficient hESC-derived cardiomyocytes develop HCM-like phenotypes (enlarged cell size, disorganized sarcomere, mitochondrial damage) that progress to heart failure features; restoration of Ca2+ homeostasis with verapamil prevents these phenotypes, indicating that elevated intracellular Ca2+ is a central mechanistic consequence of MLP deficiency. CRISPR/Cas9 knockout of CSRP3 in hESCs, differentiation to cardiomyocytes, calcium imaging, pharmacological rescue with verapamil Cell death & disease High 31406109
2020 S-nitrosylation of MLP at cysteine 79 (SNO-MLP) is elevated in hypertrophic myocardium and drives myocardial hypertrophy by promoting MLP binding to TLR3, which in turn increases TLR3–RIP3 complex formation and NLRP3 inflammasome activation; mutation of Cys79 or overexpression of GSNO reductase reduces hypertrophic growth. Biotin-switch assay for SNO detection, LC-MS/MS for SNO site identification, site-directed mutagenesis (C79 mutation), co-immunoprecipitation, siRNA knockdown of TLR3, TLR3 knockout mouse model, transverse aortic constriction model Circulation High 31902237
2019 Knockdown of CSRP3 in chicken satellite cells inhibits myotube differentiation (but not proliferation) by upregulating TGF-β/Smad3 signaling, including increased Smad3 phosphorylation, identifying CSRP3 as a positive regulator of satellite cell differentiation acting upstream of TGF-β/Smad3. siRNA knockdown, overexpression, Western blot for Smad3 phosphorylation, qRT-PCR, differentiation assays Gene Medium 30930226
2020 CSRP3/MLP interacts with LC3 protein to promote autophagosome formation; CSRP3 silencing impairs autophagy (reduced ATG5, ATG7, LC3-II, Beclin-1) and increases apoptosis via caspase-3/9 cleavage in chicken myoblasts; autophagy activation rescues the apoptotic phenotype. siRNA knockdown, co-immunoprecipitation, Western blot, pharmacological autophagy activation rescue International journal of molecular sciences Medium 31979369
2022 Vitamin C promotes nuclear translocation of CSRP3, and nuclear CSRP3 interacts directly with myogenic transcription factors MyoD and MyoG to promote muscle differentiation and repair. Transcriptomics, proteomics, co-immunoprecipitation, nuclear translocation assays in C2C12 cells and mouse muscle injury model Journal of agricultural and food chemistry Medium 35652451

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1997 MLP-deficient mice exhibit a disruption of cardiac cytoarchitectural organization, dilated cardiomyopathy, and heart failure. Cell 680 9039266
2004 Clp ATPases are required for stress tolerance, intracellular replication and biofilm formation in Staphylococcus aureus. Molecular microbiology 248 15554981
1990 Sequence and structure of Clp P, the proteolytic component of the ATP-dependent Clp protease of Escherichia coli. The Journal of biological chemistry 215 2197275
2009 Adapting the machine: adaptor proteins for Hsp100/Clp and AAA+ proteases. Nature reviews. Microbiology 188 19609260
1999 New insights into the ATP-dependent Clp protease: Escherichia coli and beyond. Molecular microbiology 169 10320569
2008 Coding sequence mutations identified in MYH7, TNNT2, SCN5A, CSRP3, LBD3, and TCAP from 313 patients with familial or idiopathic dilated cardiomyopathy. Clinical and translational science 158 19412328
2006 Recent advances in the study of Clp, FtsH and other proteases located in chloroplasts. Current opinion in plant biology 155 16603408
2001 Regulation of Streptococcus pneumoniae clp genes and their role in competence development and stress survival. Journal of bacteriology 150 11717289
1999 Lon and Clp family proteases and chaperones share homologous substrate-recognition domains. Proceedings of the National Academy of Sciences of the United States of America 150 10359771
1994 Processive degradation of proteins by the ATP-dependent Clp protease from Escherichia coli. Requirement for the multiple array of active sites in ClpP but not ATP hydrolysis. The Journal of biological chemistry 148 8027082
2014 Organization, function and substrates of the essential Clp protease system in plastids. Biochimica et biophysica acta 139 25482260
2013 Clp chaperones and proteases are central in stress survival, virulence and antibiotic resistance of Staphylococcus aureus. International journal of medical microbiology : IJMM 139 24457183
2008 Beyond the sarcomere: CSRP3 mutations cause hypertrophic cardiomyopathy. Human molecular genetics 136 18505755
1996 Degradation by proteases Lon, Clp and HtrA, of Escherichia coli proteins aggregated in vivo by heat shock; HtrA protease action in vivo and in vitro. Molecular microbiology 123 8939438
2000 CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4. The Journal of biological chemistry 122 10753915
2004 Mlp-dependent anchorage and stabilization of a desumoylating enzyme is required to prevent clonal lethality. The Journal of cell biology 120 15557117
2009 Large scale comparative proteomics of a chloroplast Clp protease mutant reveals folding stress, altered protein homeostasis, and feedback regulation of metabolism. Molecular & cellular proteomics : MCP 114 19423572
2000 The muscle regulatory and structural protein MLP is a cytoskeletal binding partner of betaI-spectrin. Journal of cell science 99 10751147
2006 A camel passes through the eye of a needle: protein unfolding activity of Clp ATPases. Molecular microbiology 93 16879409
2022 Nrf2 Deficiency Exacerbated CLP-Induced Pulmonary Injury and Inflammation through Autophagy- and NF-κB/PPARγ-Mediated Macrophage Polarization. Cells 91 36497185
2003 The CRP/MLP/TLP family of LIM domain proteins: acting by connecting. BioEssays : news and reviews in molecular, cellular and developmental biology 88 12539241
2000 Decreased expression of the cardiac LIM domain protein MLP in chronic human heart failure. Circulation 86 10851202
2016 Inhibition of hepatic cells pyroptosis attenuates CLP-induced acute liver injury. American journal of translational research 85 28078039
2014 Lactobacillus plantarum CLP-0611 ameliorates colitis in mice by polarizing M1 to M2-like macrophages. International immunopharmacology 84 24815859
2009 A common MLP (muscle LIM protein) variant is associated with cardiomyopathy. Circulation research 84 20044516
2020 SNO-MLP (S-Nitrosylation of Muscle LIM Protein) Facilitates Myocardial Hypertrophy Through TLR3 (Toll-Like Receptor 3)-Mediated RIP3 (Receptor-Interacting Protein Kinase 3) and NLRP3 (NOD-Like Receptor Pyrin Domain Containing 3) Inflammasome Activation. Circulation 81 31902237
2016 Posttranslational Control of ALA Synthesis Includes GluTR Degradation by Clp Protease and Stabilization by GluTR-Binding Protein. Plant physiology 75 26884485
2004 Fine-tuning in regulation of Clp protein content in Bacillus subtilis. Journal of bacteriology 74 14679237
2010 The Clp chaperones and proteases of the human malaria parasite Plasmodium falciparum. Journal of molecular biology 69 20887733
1999 Identification of clp genes expressed in senescing Arabidopsis leaves. Plant & cell physiology 67 10427773
2017 Conformational control of the bacterial Clp protease by natural product antibiotics. Natural product reports 64 28375422
2010 Characterization of a Clp protease gene regulator and the reaeration response in Mycobacterium tuberculosis. PloS one 63 20661284
2002 Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein. Journal of cell science 61 11973348
2021 Structure, function, and substrates of Clp AAA+ protease systems in cyanobacteria, plastids, and apicoplasts: A comparative analysis. The Journal of biological chemistry 55 33497624
2009 Structure and function of a novel type of ATP-dependent Clp protease. The Journal of biological chemistry 55 19237538
2006 Involvement of Clp protease activity in modulating the Bacillus subtilissigmaw stress response. Molecular microbiology 54 16899079
2023 Clp-targeting BacPROTACs impair mycobacterial proteostasis and survival. Cell 53 37137307
2022 Inhibition of Ferroptosis Attenuates Glutamate Excitotoxicity and Nuclear Autophagy in a CLP Septic Mouse Model. Shock (Augusta, Ga.) 52 35066511
2018 Interference with Clp protease impairs carotenoid accumulation during tomato fruit ripening. Journal of experimental botany 52 29385595
2011 MLP (muscle LIM protein) as a stress sensor in the heart. Pflugers Archiv : European journal of physiology 51 21484537
2019 MLP-deficient human pluripotent stem cell derived cardiomyocytes develop hypertrophic cardiomyopathy and heart failure phenotypes due to abnormal calcium handling. Cell death & disease 50 31406109
2015 Early severe impairment of hematopoietic stem and progenitor cells from the bone marrow caused by CLP sepsis and endotoxemia in a humanized mice model. Stem cell research & therapy 50 26272069
2019 Extreme variation in rates of evolution in the plastid Clp protease complex. The Plant journal : for cell and molecular biology 47 30570818
2016 Naringin Decreases TNF-α and HMGB1 Release from LPS-Stimulated Macrophages and Improves Survival in a CLP-Induced Sepsis Mice. PloS one 47 27716835
2003 Regulation of expression of the paralogous Mlp family in Borrelia burgdorferi. Infection and immunity 47 12933844
2004 Protein binding and disruption by Clp/Hsp100 chaperones. Structure (London, England : 1993) 46 14962378
2014 GSK-3β inhibition attenuates CLP-induced liver injury by reducing inflammation and hepatic cell apoptosis. Mediators of inflammation 45 25525303
2004 ClgR, a novel regulator of clp and lon expression in Streptomyces. Journal of bacteriology 45 15126487
1998 Clp protease complexes and their diversity in chloroplasts. Planta 45 9951729
2019 Control of plastidial metabolism by the Clp protease complex. Journal of experimental botany 44 30576524
2013 Regulation of host hemoglobin binding by the Staphylococcus aureus Clp proteolytic system. Journal of bacteriology 44 23995637
2004 Dystrophin- and MLP-deficient mouse hearts: marked differences in morphology and function, but similar accumulation of cytoskeletal proteins. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 44 15494447
2021 Contribution of the Clp Protease to Bacterial Survival and Mitochondrial Homoeostasis. Microbial physiology 43 34438398
2015 Origin of Circulating Free DNA in Sepsis: Analysis of the CLP Mouse Model. Mediators of inflammation 43 26273139
2015 Dexmedetomidine Ameliorate CLP-Induced Rat Intestinal Injury via Inhibition of Inflammation. Mediators of inflammation 43 26273145
2017 Two Isoforms of Clp Peptidase in Pseudomonas aeruginosa Control Distinct Aspects of Cellular Physiology. Journal of bacteriology 39 27849175
2017 Both Hsp70 chaperone and Clp protease plastidial systems are required for protection against oxidative stress. Plant signaling & behavior 39 28277974
2019 Knockdown of CSRP3 inhibits differentiation of chicken satellite cells by promoting TGF-β/Smad3 signaling. Gene 37 30930226
2011 Insights into the Clp/HSP100 chaperone system from chloroplasts of Arabidopsis thaliana. The Journal of biological chemistry 37 21737456
1998 Inactivation of the clpP1 gene for the proteolytic subunit of the ATP-dependent Clp protease in the cyanobacterium Synechococcus limits growth and light acclimation. Plant molecular biology 37 9678574
2020 Typically inhibiting USP14 promotes autophagy in M1-like macrophages and alleviates CLP-induced sepsis. Cell death & disease 35 32820146
2014 The Mycobacterium tuberculosis Clp gene regulator is required for in vitro reactivation from hypoxia-induced dormancy. The Journal of biological chemistry 34 25422323
2012 AsnB, regulated by diffusible signal factor and global regulator Clp, is involved in aspartate metabolism, resistance to oxidative stress and virulence in Xanthomonas oryzae pv. oryzicola. Molecular plant pathology 34 23157387
2001 Clp ATPases and their role in protein unfolding and degradation. Advances in protein chemistry 34 11868279
2000 Expression and immunological analysis of the plasmid-borne mlp genes of Borrelia burgdorferi strain B31. Infection and immunity 34 10948116
2020 Identification and functional characterization of NbMLP28, a novel MLP-like protein 28 enhancing Potato virus Y resistance in Nicotiana benthamiana. BMC microbiology 32 32143563
2015 Muscle LIM protein/CSRP3: a mechanosensor with a role in autophagy. Cell death discovery 32 27551448
2020 The Autophagy Regulatory Molecule CSRP3 Interacts with LC3 and Protects Against Muscular Dystrophy. International journal of molecular sciences 31 31979369
2018 Urotensin receptors as a new target for CLP induced septic lung injury in mice. Naunyn-Schmiedeberg's archives of pharmacology 31 30353214
2014 Nuclear pore complex evolution: a trypanosome Mlp analogue functions in chromosomal segregation but lacks transcriptional barrier activity. Molecular biology of the cell 31 24600046
2017 Coactosin-like protein CLP/Cotl1 suppresses breast cancer growth through activation of IL-24/PERP and inhibition of non-canonical TGFβ signaling. Oncogene 30 28925397
2011 XCC2731, a GGDEF domain protein in Xanthomonas campestris, is involved in bacterial attachment and is positively regulated by Clp. Microbiological research 29 21237626
2023 HN-PPISP: a hybrid network based on MLP-Mixer for protein-protein interaction site prediction. Briefings in bioinformatics 28 36403092
2018 Protective Effect of Tubastatin A in CLP-Induced Lethal Sepsis. Inflammation 28 30047002
2017 Generation and characterization of a collection of knock-down lines for the chloroplast Clp protease complex in tobacco. Journal of experimental botany 28 28369470
2011 The chloroplast ATP-dependent Clp protease in vascular plants - new dimensions and future challenges. Physiologia plantarum 28 22085372
2021 Splenectomy provides protective effects against CLP-induced sepsis by reducing TRegs and PD-1/PD-L1 expression. The international journal of biochemistry & cell biology 27 33774183
2019 Novel Chitohexaose Analog Protects Young and Aged mice from CLP Induced Polymicrobial Sepsis. Scientific reports 25 30814582
2018 Inhibition of the mTOR Pathway Exerts Cardioprotective Effects Partly through Autophagy in CLP Rats. Mediators of inflammation 25 30050390
2001 Outer membrane protein A (OmpA), peptidoglycan-associated lipoprotein (PAL), and murein lipoprotein (MLP) are released in experimental Gram-negative sepsis. Journal of endotoxin research 25 11521086
2019 IL-27 is elevated in sepsis with acute hepatic injury and promotes hepatic damage and inflammation in the CLP model. Cytokine 24 31786500
2003 ALP and MLP distribution during myofibrillogenesis in cultured cardiomyocytes. Cell motility and the cytoskeleton 24 12589684
2020 The HDL from septic-ARDS patients with composition changes exacerbates pulmonary endothelial dysfunction and acute lung injury induced by cecal ligation and puncture (CLP) in mice. Respiratory research 23 33148285
2017 Tussilagone Inhibits the Inflammatory Response and Improves Survival in CLP-Induced Septic Mice. International journal of molecular sciences 23 29258263
2017 Dexmedetomidine mitigates CLP-stimulated acute lung injury via restraining the RAGE pathway. American journal of translational research 23 29312480
2013 Anti-inflammatory activities of isorhamnetin-3-O-galactoside against HMGB1-induced inflammatory responses in both HUVECs and CLP-induced septic mice. Journal of cellular biochemistry 23 22930571
2019 The cardiac syndecan-4 interactome reveals a role for syndecan-4 in nuclear translocation of muscle LIM protein (MLP). The Journal of biological chemistry 22 30967474
2019 Lactobacillus rhamnosus GG Ameliorates Liver Injury and Hypoxic Hepatitis in Rat Model of CLP-Induced Sepsis. Digestive diseases and sciences 21 31049763
2015 The E3 ligase AtCHIP positively regulates Clp proteolytic subunit homeostasis. Journal of experimental botany 21 26085677
2013 Increased expression of clp genes in Lactobacillus delbrueckii UFV H2b20 exposed to acid stress and bile salts. Beneficial microbes 19 24311319
2001 Expression of clpX, an ATPase subunit of the Clp protease, is heat and cold shock inducible in Lactococcus lactis. Journal of dairy science 19 11518300
2022 Proteomics, phylogenetics, and coexpression analyses indicate novel interactions in the plastid CLP chaperone-protease system. The Journal of biological chemistry 18 35065075
2022 Toll-like receptor 4-mediated endoplasmic reticulum stress induces intestinal paneth cell damage in mice following CLP-induced sepsis. Scientific reports 18 36088483
2009 Induction of CRP3/MLP expression during vein arterialization is dependent on stretch rather than shear stress. Cardiovascular research 18 19351738
2008 Regulation of the pehA gene encoding the major polygalacturonase of Xanthomonas campestris by Clp and RpfF. Microbiology (Reading, England) 18 18310017
2018 First identification of homozygous truncating CSRP3 variants in two unrelated cases with hypertrophic cardiomyopathy. Gene 17 30012424
2017 Effect of Triggering Receptor Expressed on Myeloid Cells 1 (TREM-1) Blockade in Rats with Cecal Ligation and Puncture (CLP)-Induced Sepsis. Medical science monitor : international medical journal of experimental and clinical research 17 29059148
2012 Cardiac remodeling is not modulated by overexpression of muscle LIM protein (MLP). Basic research in cardiology 17 22421737
2022 Vitamin C Promotes Muscle Development Mediated by the Interaction of CSRP3 with MyoD and MyoG. Journal of agricultural and food chemistry 16 35652451
2009 Clp and RpfF up-regulate transcription of pelA1 gene encoding the major pectate lyase in Xanthomonas campestris pv. campestris. Journal of agricultural and food chemistry 16 19601664