Affinage

CPEB1

Cytoplasmic polyadenylation element-binding protein 1 · UniProt Q9BZB8

Length
566 aa
Mass
62.6 kDa
Annotated
2026-06-09
100 papers in source corpus 48 papers cited in narrative 48 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CPEB1 is a sequence-specific RNA-binding protein that governs cytoplasmic polyadenylation-induced translation by recognizing cytoplasmic polyadenylation elements (CPEs, UUUUUAU) in the 3' UTRs of target mRNAs and nucleating a regulatory ribonucleoprotein complex on them (PMID:7954828, PMID:8665866). RNA recognition requires its tandem RRMs together with a cysteine-histidine zinc finger, which fold into a closure-competent arrangement that achieves high-fidelity CPE recognition and positions the protein for complex assembly (PMID:9447964, PMID:24990967). On bound mRNAs CPEB1 organizes a polyadenylation apparatus that includes the scaffold symplekin, CPSF, and the poly(A) polymerase GLD-2, while in the repressed state it engages eIF4E-binding factors (4E-T, the oocyte-specific eIF4E1b) and recruits the CCR4-NOT deadenylase to keep target mRNAs translationally silent (PMID:11106762, PMID:15550246, PMID:17942399, PMID:36096799). The molecular switch from repression to activation is a single Aurora A (Eg2/IAK1)-catalyzed phosphorylation of a critical serine that triggers CPSF recruitment and complex remodeling; this event is integrated by additional kinases (MAPK, Cdc2, Plx1) and reversed by PP1, while subsequent SCF(beta-TrCP)-mediated, PEST/TSG-motif-dependent ubiquitination drives partial CPEB1 destruction to impose temporal order on successive polyadenylation events (PMID:11106762, PMID:17986610, PMID:17344432, PMID:12815066, PMID:11927567). Through this mechanism CPEB1 is essential for oocyte meiotic maturation and germ cell differentiation, controlling polyadenylation of c-mos, cyclin, synaptonemal-complex, and Gdf9 mRNAs, and acts within a CPEB1→CPEB4 translational relay during the meiotic divisions (PMID:8665866, PMID:11702780, PMID:17050619, PMID:20531391). Beyond meiosis, CPEB1 drives mitotic cell-cycle progression and localizes target mRNAs and translation machinery to the mitotic spindle (PMID:20364142, PMID:33323527), localizes and translates dendritic mRNAs such as alpha-CaMKII at activated synapses (PMID:9856468, PMID:11980711, PMID:12629046), enforces cellular senescence via translational control of p53 and c-myc (PMID:17015432, PMID:19141477), regulates innate immune output through a TAK1/NF-κB/IL-6 axis (PMID:25452303), and maintains epithelial polarity through apical localization of ZO-1 mRNA, the loss of which promotes EMT and metastasis (PMID:22334078, PMID:26411364). CPEB1 also shuttles into the nucleus via CRM1-dependent export and there directs alternative polyadenylation (3' UTR shortening) and alternative splicing by blocking U2AF65 recruitment (PMID:20040591, PMID:23434754). Genetically, loss of CPEB1 ameliorates the phenotypes of Fragile X syndrome, where CPEB1 and FMRP act in opposing control of translational elongation (PMID:24141422).

Mechanistic history

Synthesis pass · year-by-year structured walk · 18 steps
  1. 1994 High

    Established that a dedicated factor is required for cytoplasmic polyadenylation, answering whether CPE-directed poly(A) addition needs a sequence-specific binding protein.

    Evidence RNA affinity chromatography, immunodepletion/add-back, and in vitro polyadenylation reconstitution in Xenopus egg extracts

    PMID:7954828

    Open questions at the time
    • Did not define the target mRNA repertoire
    • No structural basis for CPE recognition
  2. 1996 High

    Defined CPEB1's physiological mRNA targets and showed its activity is required for meiotic maturation, linking polyadenylation to a developmental decision.

    Evidence In vitro polyadenylation, antibody injection into oocytes, immunoprecipitation of cyclin/c-mos mRNAs

    PMID:8665866

    Open questions at the time
    • Did not reveal how CPEB1 activity is switched on
    • Mechanism coupling polyadenylation to translation unresolved
  3. 1998 High

    Mapped the RNA-binding determinants, showing both RRMs and a zinc finger are required and that CPEB1 binds as a monomer.

    Evidence Deletion/point mutagenesis with RNA gel-shift and metal chelation/supplementation

    PMID:9447964

    Open questions at the time
    • No atomic structure
    • Did not address protein-protein contacts
  4. 2000 High

    Identified the activating switch: Aurora A phosphorylation of a single serine recruits CPSF to build an active polyadenylation complex, the most proximal trigger for polyadenylation.

    Evidence In vitro kinase assay, co-IP, reconstitution with purified components

    PMID:11106762

    Open questions at the time
    • Did not explain temporal ordering of distinct target mRNAs
    • Upstream control of Aurora A unaddressed
  5. 2001 High

    Demonstrated CPEB1 is essential in vivo for germ cell differentiation by controlling synaptonemal-complex protein mRNA translation, extending the mechanism to mammalian development.

    Evidence CPEB knockout mouse, polysome fractionation, RNA co-IP, electron microscopy

    PMID:11526086 PMID:11702780

    Open questions at the time
    • Full target mRNA set in vivo not defined
    • Somatic roles untested
  6. 2001 High

    Showed CPEB1 abundance is actively downregulated via PEST-dependent ubiquitin-proteasome degradation, introducing termination as part of the regulatory cycle.

    Evidence Proteasome/ubiquitin inhibitors, PEST deletion, metabolic labeling

    PMID:11237472

    Open questions at the time
    • E3 ligase not identified
    • Kinase signals targeting degradation unknown
  7. 2003 High

    Resolved the timing logic: sequential kinase (MAPK, Cdc2) and phosphatase (PP1) inputs and Cdc2/PEST-dependent partial destruction order successive polyadenylation events during meiosis.

    Evidence Phospho-specific antibodies across staged oocytes, kinase inhibitors, PEST mutagenesis, polyadenylation time-courses

    PMID:11927567 PMID:12815066 PMID:17344432

    Open questions at the time
    • Integration of multiple phosphosites quantitatively unresolved
    • PP1 targeting specificity unclear
  8. 2002 High

    Extended CPEB1-directed polyadenylation to neurons, establishing activity-dependent local translation at synapses downstream of NMDA receptor/Aurora signaling.

    Evidence Synaptic fractionation, immunofluorescence, NMDA stimulation with phosphorylation assay, oocyte injection

    PMID:11980711 PMID:12629046 PMID:9856468

    Open questions at the time
    • In vivo behavioral consequences not yet defined
    • Motor-coupling mechanism for transport partially characterized
  9. 2004 High

    Identified the core complex scaffold (symplekin) and poly(A) polymerase (GLD-2), and placed upstream signaling (insulin/PI3K, GSK-3) on Aurora A activity, building the biochemical architecture of the machine.

    Evidence Co-IP, fractionation, depletion reconstitution in Xenopus extracts; in vitro kinase assays with Aurora A mutants

    PMID:14724178 PMID:15550246

    Open questions at the time
    • Stoichiometry and order of assembly not fully resolved
    • How signals converge spatially unclear
  10. 2007 High

    Defined the repressive state and termination machinery: CPEB1 partners with eIF4E-binding factors (4E-T, eIF4E1b) to silence mRNAs, ePAB dynamically hands off to eIF4G, and SCF(beta-TrCP) recognizes a phospho-TSG motif for degradation.

    Evidence Reciprocal co-IP, tethered reporter assays, sequential in vitro kinase assays (Cdc2, Plx1), TSG-motif mutagenesis, antibody injection

    PMID:17938241 PMID:17942399 PMID:17986610

    Open questions at the time
    • Quantitative kinetics of activation-to-repression switching unresolved
    • Coordination of degradation with translation activation incomplete
  11. 2006 High

    Showed CPEB1 is required for cellular senescence through translational control of c-myc, connecting it to tumor-suppressor pathways in somatic cells.

    Evidence Knockout MEFs with add-back rescue, epistasis with p53/p19ARF/p16, polysome fractionation

    PMID:17015432

    Open questions at the time
    • Direct vs. indirect control of c-myc translation not fully separated
    • Relationship to p53 control resolved only later
  12. 2009 High

    Established translational control of p53 mRNA polyadenylation as a senescence mechanism in human fibroblasts, with metabolic consequences.

    Evidence shRNA knockdown with rescue, poly(A) tail assay, polysome fractionation, respirometry

    PMID:19141477 PMID:21478871

    Open questions at the time
    • Opposing directionality of p53 control between studies (miR-122/Gld4 axis) not fully reconciled
    • Tissue specificity uncharacterized
  13. 2010 High

    Generalized CPEB1's polyadenylation mechanism to mitotic cell-cycle progression and defined a CPEB1→CPEB4 translational relay, distinguishing paralog roles.

    Evidence RNAi of CPEB1/CPEB4, poly(A) tail assays, cell-cycle FACS, reporter and poly(A) assays during meiosis

    PMID:20364142 PMID:20531391

    Open questions at the time
    • Spindle-specific transcript targets not enumerated here
    • Mechanism of CPEB1-to-CPEB4 handoff partially defined
  14. 2012 High

    Uncovered a nuclear function: CPEB1 shuttles via CRM1 and directs alternative polyadenylation and splicing genome-wide by blocking U2AF65, broadening its mechanism beyond cytoplasmic translation.

    Evidence CRM1 inhibition, NES mutagenesis, nuclear fractionation, RNA-seq/3' end sequencing, U2AF65 co-IP

    PMID:18923137 PMID:20040591 PMID:23434754

    Open questions at the time
    • How nuclear vs. cytoplasmic activities are partitioned unclear
    • Determinants of co-transcriptional recruitment unknown
  15. 2012 High

    Linked CPEB1 to epithelial polarity through apical ZO-1 mRNA localization, with loss driving EMT and metastasis, establishing a tumor-relevant function.

    Evidence Knockdown/rescue with CPE-mutant ZO-1 mRNA, 3D mammary culture, FISH, in vivo metastasis model, MMP9 poly(A) assay

    PMID:22334078 PMID:26411364

    Open questions at the time
    • Mechanism of apical mRNA targeting incompletely defined
    • Relative contribution of multiple targets to EMT unresolved
  16. 2014 High

    Determined solution structures of the tandem RRMs and assigned the ZZ domain dual RNA/protein roles, providing the structural basis for high-fidelity CPE recognition and complex nucleation.

    Evidence Solution NMR of free and RNA-bound RRMs with ZZ-domain functional mutagenesis

    PMID:24990967

    Open questions at the time
    • Full-length complex structure absent
    • Structural basis of phospho-regulation not captured
  17. 2014 High

    Placed CPEB1 in innate immunity as a translational brake on TAK1, controlling NF-κB/IL-6 output, and in Fragile X via opposing FMRP at translational elongation.

    Evidence Knockout mice, macrophage LPS stimulation, double depletion, TAK1 inhibitor in vivo; Fmr1/Cpeb1 double-knockout phenotypic rescue

    PMID:25452303 PMID:28...

    Open questions at the time
    • IL-6 transcriptional vs. translational contributions partially separated
    • Disease-relevant target hierarchy unclear
  18. 2022 High

    Distinguished CPEB1 mechanistically from paralogs and identified phosphorylation-driven RNP remodeling and CCR4-NOT recruitment, while extending function to muscle stem cell activation via Myod1 translation.

    Evidence Comparative RNP fractionation, RNA-seq, phosphorylation and phase-separation assays; quantitative proteomics with Myod1 3' UTR CPE binding and genetic mouse models

    PMID:35177647 PMID:36096799

    Open questions at the time
    • Whether CPEB1 undergoes phase separation like CPEB2-4 unresolved
    • In vivo target sets across tissues incomplete

Open questions

Synthesis pass · forward-looking unresolved questions
  • How CPEB1's nuclear (alternative processing) and cytoplasmic (polyadenylation/translation) activities are coordinated, and what determines target and tissue specificity across its many contexts, remains unresolved.
  • No unified model partitioning nuclear vs. cytoplasmic functions
  • Full-length RNP complex structure unavailable
  • Tissue-specific target codes undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 5 GO:0045182 translation regulator activity 5 GO:0060090 molecular adaptor activity 3 GO:0140098 catalytic activity, acting on RNA 3
Localization
GO:0005634 nucleus 3 GO:0005815 microtubule organizing center 2 GO:0005829 cytosol 2 GO:0005856 cytoskeleton 2 GO:0005730 nucleolus 1
Pathway
R-HSA-1474165 Reproduction 4 R-HSA-8953854 Metabolism of RNA 4 R-HSA-1640170 Cell Cycle 3 R-HSA-8953897 Cellular responses to stimuli 3 R-HSA-74160 Gene expression (Transcription) 2
Partners
4E-TAURKACPSFEIF4E1BEPABGLD-2MASKINSYMPLEKIN
Complex memberships
CCR4-NOT deadenylase complex (recruited)CPEB1 cytoplasmic polyadenylation complex (CPEB1-CPSF-symplekin-GLD-2)

Evidence

Reading pass · 48 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1994 CPEB (CPEB1) was identified as a sequence-specific RNA-binding protein required for cytoplasmic polyadenylation of CPE-containing maternal mRNAs in Xenopus oocytes. Immunodepletion of CPEB from polyadenylation-competent egg extracts abolished cytoplasmic polyadenylation; addition of recombinant CPEB partially restored activity. CPEB contains two RNA recognition motifs (RRMs) and is phosphorylated during oocyte maturation at a time corresponding with induction of polyadenylation. RNA affinity chromatography, immunodepletion, in vitro polyadenylation reconstitution, cDNA cloning Cell High 7954828
1996 CPEB binds CPE sequences in the 3' UTRs of cyclin A1, B1, B2, cdk2, c-mos, and G10 mRNAs and is necessary for cytoplasmic polyadenylation of these mRNAs in egg extracts. Injection of CPEB antibody into oocytes inhibits polyadenylation and translational activation of c-mos mRNA and blocks progesterone-induced meiotic maturation. In vitro polyadenylation assay with egg extracts, antibody injection into oocytes, immunoprecipitation The EMBO journal High 8665866
1998 Both RRM domains and a cysteine-histidine zinc finger region of CPEB are essential for RNA binding. Deletion of either RRM greatly reduces RNA binding; deletion of the zinc finger abolishes it. Single alanine substitutions of specific cysteines or histidines within the zinc finger also abolish RNA binding. CPEB binds RNA as a monomer; zinc chelation inhibits binding and zinc supplementation restores it. E. coli overexpression of deletion/point mutants, RNA gel-shift assays, metal chelation/supplementation experiments Molecular and cellular biology High 9447964
1998 CPEB is present in dendritic layers of hippocampus, at synapses in cultured neurons, and in postsynaptic densities. CPEB binds CPE sequences in the 3' UTR of alpha-CaMKII mRNA, and these CPEs mediate polyadenylation-induced translation in Xenopus oocytes. In the intact brain, visual experience induces alpha-CaMKII mRNA polyadenylation and translation. Immunohistochemistry, postsynaptic density fractionation, RNA injection into Xenopus oocytes, in vivo polyadenylation assay Neuron High 9856468
2000 CPEB and maskin colocalize with cyclin B1 mRNA at the mitotic apparatus (spindle/centrosome) of Xenopus blastomeres. CPEB interacts with microtubules and is required for localization of cyclin B1 mRNA to the mitotic apparatus. Agents disrupting polyadenylation-induced translation inhibit cell division and cause spindle and centrosome defects. Immunofluorescence/colocalization, injection of translation inhibitory agents, RNA localization assays in embryos Cell High 11081630
2000 Eg2 (Aurora A) kinase phosphorylates CPEB serine 174, and this phosphorylation event is the most proximal stimulus for cytoplasmic polyadenylation. Phosphorylated CPEB directly recruits CPSF (via its 160 kDa subunit) into an active polyadenylation complex. This interaction does not require RNA tethering. Cytoplasmic polyadenylation was reconstituted in vitro with purified components. In vitro kinase assay, co-immunoprecipitation, in vitro polyadenylation reconstitution with purified components Molecular cell High 11106762
2001 CPEB knockout female mice have vestigial ovaries devoid of oocytes; oocytes arrest at pachytene in both male and female knockout embryos. Two synaptonemal complex protein mRNAs containing CPEs interact with CPEB in vitro and in vivo, have shortened poly(A) tails, and fail to associate with polysomes in null mice. Synaptonemal complexes are absent in knockout animals, establishing that CPEB controls germ cell differentiation by regulating synaptonemal complex protein mRNA translation. Knockout mouse, polysome fractionation, RNA co-immunoprecipitation, electron microscopy for synaptonemal complex Developmental cell High 11702780
2001 In mouse oocytes, the murine Aurora A homolog IAK1/Eg2 phosphorylates CPEB on the critical regulatory residue, and this is essential for cytoplasmic polyadenylation and meiotic progression. A dominant-negative non-phosphorylatable CPEB prevents polyadenylation. IAK1/Eg2 inhibitory peptide blocks meiotic progression in injected oocytes. Immunohistochemistry in mouse oocytes, injection of IAK1/Eg2-inhibitory peptide, dominant-negative CPEB injection Development (Cambridge, England) High 11526086
2001 CPEB degradation during Xenopus oocyte maturation requires a 14 amino acid PEST domain and occurs via the ubiquitin-proteasome pathway. Approximately 75% of CPEB is degraded coincident with germinal vesicle breakdown; proteasome and ubiquitination inhibitors block this degradation. Proteasome/ubiquitin inhibitor treatment, CPEB PEST domain deletion analysis, metabolic labeling to measure synthesis vs. accumulation Developmental biology High 11237472
2002 NMDA receptor activation at hippocampal synapses triggers Aurora kinase phosphorylation of CPEB, which is required for cytoplasmic polyadenylation and translation of alpha-CaMKII mRNA at synaptic sites. All polyadenylation machinery components (CPEB, maskin, PAP, CPSF, Aurora) are present at synapses. Immunofluorescence/colocalization at synapses, NMDA stimulation with phosphorylation assay, synaptic fractionation The EMBO journal High 11980711
2002 Cdc2-mediated phosphorylation of CPEB via a PEST box triggers partial CPEB destruction by the proteasome during metaphase I in Xenopus oocytes. This partial destruction is required for the temporal activation of cyclin B1 mRNA polyadenylation (at metaphase I, after Mos mRNA) and for meiotic progression to metaphase II. CPEB destruction is also required for mitosis in early embryos. In vivo phosphorylation analysis, proteasome inhibition, CPEB PEST domain mutagenesis, mRNA polyadenylation time-course assays The EMBO journal High 11927567
2003 CPEB facilitates mRNA transport to dendrites via RNA-containing particles that move in a microtubule-dependent manner (4–8 µm/min). CPEB-GFP particles contain maskin, dynein, and kinesin. The CPE is sufficient for dendritic targeting; a CPEB mutant defective for motor interaction inhibits transport; in CPEB knockout neurons, dendritic transport of a CPE-containing reporter RNA is reduced. Live imaging of CPEB-GFP particles in rat hippocampal neurons, motor protein co-immunoprecipitation, CPEB knockout neurons, recombinant virus-mediated CPE reporter transport assay Genes & development High 12629046
2004 Symplekin and xGLD-2 are essential factors for CPEB-mediated cytoplasmic polyadenylation in Xenopus oocytes. Symplekin serves as a scaffold that binds both CPEB and CPSF. xGLD-2, an atypical poly(A) polymerase, is anchored to CPEB and CPSF even before polyadenylation begins. Co-immunoprecipitation, biochemical fractionation, functional depletion assays in Xenopus egg extracts Cell High 15550246
2004 Progesterone and insulin both stimulate Aurora A (Eg2)-catalyzed CPEB serine 174 phosphorylation and cytoplasmic polyadenylation; the insulin pathway acts through PI3K/PKC-zeta upstream of Aurora A. The intersection of progesterone and insulin pathways occurs at GSK-3, which inhibits Aurora A by phosphorylating it on S290/291. GSK-3-phosphorylated Aurora A has reduced capacity to phosphorylate CPEB; constitutively active Aurora A (S290/291A) rescues polyadenylation. In vitro kinase assay, GSK-3/Aurora A co-immunoprecipitation, Aurora A mutagenesis (S290/291A, S290/291D), PI3K/PKC inhibitors in oocytes Genes & development High 14724178
2005 All mammalian CPEB1 isoforms associate with stress granules and dcp1 bodies in the cytoplasm; this association requires the RNA-binding ability of CPEB1, while the Aurora A phosphorylation site is dispensable. Transient CPEB1 overexpression induces assembly of stress granules, which then recruit dcp1 bodies. rck/p54 DEAD-box protein (a known CPEB partner) is present in both structures. Immunofluorescence, CPEB1 RNA-binding mutant analysis, transient transfection/overexpression assay Journal of cell science Medium 15731006
2006 CPEB knockout mouse embryo fibroblasts (MEFs) fail to undergo senescence in culture and are immortal; exogenous CPEB restores senescence. CPEB cannot stimulate senescence when p53, p19ARF, or p16INK4A are absent. CPEB acts as a translational repressor of c-myc mRNA; unregulated Myc translation in CPEB-null MEFs may cause bypass of senescence. Ras cannot induce senescence in MEFs lacking CPEB. CPEB knockout MEFs, reintroduction of exogenous CPEB, genetic epistasis with p53/p19ARF/p16 knockout, polysome fractionation for c-myc mRNA Genes & development High 17015432
2006 RINGO/Spy mRNA translation is required upstream of CPEB-directed polyadenylation in Xenopus oocytes. Pumilio 2 (Pum2) binds Pumilio-Binding Elements in the RINGO/Spy 3' UTR and represses its translation in immature oocytes; this repression also involves XDAZL and ePAB. After induction of maturation, Pum2 dissociates from RINGO/Spy mRNA, allowing RINGO/Spy synthesis, which is then required for CPEB activation. Pum2 co-immunoprecipitates with XDAZL and ePAB. mRNA reporter assays, deletion analysis of 3' UTR, co-immunoprecipitation, mRNA injection into oocytes Genes & development High 16418484
2007 CPEB in early Xenopus oocytes interacts (protein-protein) with Xp54 RNA helicase, P100/Pat1, RAP55, the eIF4E-binding protein 4E-T, and an oocyte-specific eIF4E isoform (eIF4E1b) — but not with eIF4E1a, eIF4G, or the late-oogenesis factors maskin, PARN, or 4E-BP1. eIF4E1b binds m7GTP weakly and associates with 4E-T (not eIF4G). Tethered 4E-T or eIF4E1b represses translation in a cap-dependent manner. Injection of eIF4E1b antibody accelerates meiotic maturation. Co-immunoprecipitation, gel filtration, pulldown assays, cap-binding assay, tethered reporter translation assay, antibody injection into oocytes The Journal of biological chemistry High 17942399
2007 ePAB (embryonic poly(A)-binding protein) transiently associates with the CPEB polyadenylation complex; it initially interacts with CPEB, then shifts to bind the poly(A) tail after polyadenylation. Dissociation of ePAB from CPEB is regulated by RINGO/cdk1, which phosphorylates CPEB. Poly(A)-bound ePAB interacts with eIF4G to initiate translation of CPEB-bound mRNAs. Co-immunoprecipitation, in vitro kinase assay, poly(A) tail protection assays, translation initiation complex analysis Genes & development High 17938241
2007 CPEB degradation during Xenopus oocyte maturation is mediated by the SCF(beta-TrCP) E3 ubiquitin ligase. beta-TrCP binds a TSG motif (residues 190–195) of CPEB. This binding requires phosphorylation of Thr-190, Ser-191, and Ser-195; Ser-191 is phosphorylated by Polo-like kinase Plx1, which itself binds CPEB at Thr-125 pre-phosphorylated by Cdc2. Cdc2-mediated phosphorylation of multiple Ser residues is required for Thr-125 phosphorylation and beta-TrCP binding. In vitro kinase assays (Cdc2, Plx1), co-immunoprecipitation, site-directed mutagenesis of TSG motif, ubiquitin-proteasome pathway assays Proceedings of the National Academy of Sciences of the United States of America High 17986610
2007 MAPK is required for early CPEB phosphorylation in Xenopus oocytes during meiotic resumption. MAPK directly phosphorylates CPEB on four residues (T22, T164, S184, S248) but not on S174 (the Aurora A site). XGef (a Rho-GEF) co-immunoprecipitates with MAPK, and this complex can phosphorylate CPEB. Kinase inhibitors (U0126 for MAPK; ZM447439 for Aurora A/B), in vitro MAPK phosphorylation of CPEB, co-immunoprecipitation of XGef-MAPK complex Journal of cell science High 17344432
2008 CPEB-regulated translation is essential for cellular senescence of human diploid fibroblasts. CPEB knockdown allows cells to bypass senescence (M1 crisis). Knockdown cells have fewer mitochondria, reduced respiration and ROS, normal ATP, and enhanced glycolysis (Warburg-like phenotype). CPEB promotes polyadenylation of p53 mRNA 3' UTR CPEs; CPEB-depleted cells have shortened p53 poly(A) tails and ~50% reduced p53 protein, and a ~50% shRNA-mediated reduction in p53 also extends cellular lifespan. CPEB shRNA knockdown in human fibroblasts, add-back rescue, poly(A) tail length assay, polysome fractionation, mitochondria counting, respirometry Genes & development High 19141477
2008 CPEB-1 controls translation of c-Jun mRNA through CPEs in its 3' UTR; reduced c-Jun leads to lower GH transcription and reduced GH signaling (phospho-JAK2/phospho-STAT3) in CPEB-1 knockout hippocampus. CPEB-1 co-immunoprecipitates c-Jun RNA in vivo and binds c-Jun 3' UTR CPEs in vitro. Growth hormone restores LTP in hippocampal slices from WT but not KO mice with reduced magnitude. CPEB-1 knockout mice, proteomics comparison, co-immunoprecipitation of RNA, in vitro CPE binding assay, electrophysiology (LTP), pharmacological polyadenylation inhibition The Journal of neuroscience High 18716208
2008 CPEB RNA-binding protein regulates beta-catenin mRNA translation in astrocytes via a CPE in the beta-catenin 3' UTR. A dominant-negative CPEB blocks beta-catenin localization to the leading edge of migrating astrocytes and inhibits directed cell migration. In vitro wound-healing assay, dominant-negative CPEB expression, identification of CPE in beta-catenin 3' UTR, immunofluorescence Glia Medium 18618654
2008 CPEB1 shuttles between nucleus and cytoplasm via the CRM1-dependent export pathway and in the nucleus associates with lampbrush chromosomes in Xenopus oocytes and with proteins involved in nuclear RNA processing. CPEB interacts with Maskin in the nucleus and with CPE-containing mRNAs. CPEB also directly or indirectly mediates alternative splicing of at least one pre-mRNA. Leptomycin B-mediated CRM1 inhibition, nuclear fractionation, immunofluorescence on lampbrush chromosomes, RNA immunoprecipitation, alternative splicing assays RNA (New York, N.Y.) High 20040591
2008 CPEB1 accumulates in Crm1 nucleolar bodies (CNoBs) in the nucleus; export depends on two redundant NES motifs recognized by Crm1. CNoBs depend on RNA polymerase I activity. CPEB1 continuously shuttles between nucleus and cytoplasm. Crm1 inhibition (leptomycin B), NES mutagenesis, Pol I inhibition, live imaging, immunofluorescence Molecular biology of the cell Medium 18923137
2010 CPEB1 and CPEB4 are required for mitotic cell-cycle progression; loss of both causes defective entry into M phase in mitotically dividing somatic cells. Phase-specific changes in poly(A) tail length of target mRNAs mediated by CPEB1 and CPEB4 are required for cell proliferation, extending the cytoplasmic polyadenylation mechanism to general mitotic regulation. RNAi knockdown of CPEB1/CPEB4, poly(A) tail length assays, cell cycle analysis by FACS in mitotically dividing cells Nature cell biology High 20364142
2010 CPEB1 activates translation of CPEB4 mRNA, generating a positive translational loop during meiosis. CPEB1 is degraded during the first meiotic division; CPEB4 then replaces CPEB1 and drives the metaphase I to metaphase II transition. CPEB1 and CPEB4 are differentially regulated by phase-specific kinases. In vivo poly(A) tail length assays, reporter translation assays, in vivo CPEB1/CPEB4 expression analysis during meiotic progression The EMBO journal High 20531391
2011 CPEB controls IL-6 production at both translational and transcriptional levels in MEFs; CPEB-deficient cells produce large amounts of IL-6 due to improper NF-κB p65 phosphorylation and nuclear retention. IL-6-promoted senescence requires p53; CPEB knockout MEFs produce only ~50% of p53 protein and cannot respond to IL-6 for senescence. CPEB knockout MEFs, NF-κB nuclear localization assay, IL-6 ELISA, p65 phosphorylation analysis Molecular and cellular biology Medium 21536657
2011 CPEB depletion surprisingly promotes p53 mRNA polyadenylation/translation and premature senescence in fibroblasts through a miR-122/CPEB/Gld4 axis. Gld2 depletion destabilizes miR-122 (which has two binding sites in the CPEB 3' UTR), thereby elevating CPEB mRNA translation. A second poly(A) polymerase, Gld4, mediates p53 mRNA polyadenylation/translation in a CPEB-dependent manner. siRNA depletion, poly(A) tail length assay, antagomir of miR-122, 3' UTR reporter assays, cellular senescence assay Nature High 21478871
2012 CPEB1 shuttles to the nucleus where it co-localizes with splicing factors and mediates shortening of hundreds of mRNA 3' UTRs (alternative polyadenylation). CPEB1 binding to pre-mRNAs directs use of alternative polyadenylation sites and alters alternative splicing by preventing U2AF65 recruitment. CPEB1-mediated 3' UTR shortening correlates with cell proliferation and tumorigenesis. Nuclear-cytoplasmic fractionation, immunofluorescence with splicing factor markers, RNA-seq, 3' end sequencing, U2AF65 co-immunoprecipitation, reporter assays Nature High 23434754
2012 CPEB mediates apical localization of ZO-1 mRNA in mammary epithelial cells through CPE binding sites in the ZO-1 3' UTR. CPEB depletion disrupts ZO-1 apical localization, tight-junction distribution, and lumen formation in 3D culture. Rescue with ZO-1 mRNA containing CPEB-binding sites (but not without) restores cavity formation. RNAi knockdown, ectopic CPEB expression, 3D mammary culture, immunofluorescence, RNA localization by FISH, rescue with wild-type vs. CPE-mutant ZO-1 mRNA Nature communications High 22334078
2013 FMRP and CPEB1 maintain translational homeostasis at the level of polypeptide elongation. Fmr1(-/y); Cpeb1(-/-) double-knockout mice show amelioration of biochemical, morphological, electrophysiological, and behavioral phenotypes of Fragile X syndrome. Acute hippocampal depletion of CPEB1 in adult Fmr1(-/y) mice rescues working memory deficits. Double knockout mice, behavioral testing, electrophysiology, western blot, acute CPEB1 knockdown in adult mice Nature medicine High 24141422
2014 Solution structures of the tandem RRMs of human CPEB1 (and CPEB4) in free and RNA-bound states reveal an unprecedented RRM arrangement in the free state that undergoes a closure motion upon RNA binding for high-fidelity CPE recognition. The ZZ (zinc-binding) domain of CPEB1 contributes to both protein-protein and protein-RNA interactions, enabling optimal positioning of N-terminal and ZZ domains for ribonucleoprotein complex nucleation at 3' UTRs. Solution NMR structure determination of free and RNA-bound RRMs, functional mutagenesis of ZZ domain, binding assays Genes & development High 24990967
2014 CPEB regulates translation of TAK1 mRNA in macrophages; CPEB-depleted macrophages have elevated TAK1 protein, leading to prolonged NF-κB nuclear retention and high IL-6 production upon LPS stimulation. CPEB/TAK1 double depletion rescues elevated IL-6. CPEB knockout mice show LPS hypersensitivity with excess IL-6 and cytokines, which is mitigated by the TAK1 inhibitor oxozeaenol. CPEB KO mice, macrophage LPS stimulation, CPEB/TAK1 double depletion, NF-κB nuclear localization, IL-6 ELISA, TAK1 inhibitor in vivo Molecular and cellular biology High 25452303
2015 CPEB1 depletion in mammary epithelial cells causes EMT and metastasis to the lung. CPEB1-depleted cells have MMP9 mRNA with a longer poly(A) tail and enhanced MMP9 translation. Ectopic CPEB1 prevents metastasis. CPEB1 mediates apical ZO-1 mRNA localization required for cell polarity, and loss of this polarity drives EMT. CPEB1 RNAi in mammary cells, mouse fat pad injection for in vivo metastasis, poly(A) tail assay for MMP9 mRNA, polysome fractionation, ectopic CPEB1 rescue Oncogene High 26411364
2015 CPEB1 activation promotes alternative nuclear processing of VEGF and CPEB4 mRNAs (deleting translation repressor elements in their 3' UTRs). Subsequently, CPEB4 promotes cytoplasmic polyadenylation of VEGF mRNA, increasing its translation. CPEB1 or CPEB4 knockdown in mice prevents VEGF overexpression and pathologic mesenteric angiogenesis after portal vein ligation. siRNA knockdown, luciferase reporter assay, poly(A) tail assay, 3' RACE, Matrigel tube formation assay, CPEB-deficient mice with portal vein ligation Gastroenterology High 26627607
2016 Human CPEB1, when ectopically expressed in non-infected cells, recapitulates infection-related post-transcriptional changes (alternative splicing, 3' UTR shortening, poly(A) tail lengthening) caused by HCMV infection. CPEB1 is required for poly(A) tail lengthening of viral RNAs important for productive infection; CPEB1 depletion decreases productive HCMV titers and reverses infection-related cytopathology. CPEB1 ectopic expression, CPEB1 siRNA depletion, transcriptome-wide RNA-seq, poly(A) tail length assay, viral titer measurement Nature structural & molecular biology High 27775709
2018 CPEB1 directly binds the 3' UTR of SIRT1 mRNA (via CPE sites), controls its poly(A) tail length, and suppresses SIRT1 translation, thereby reducing hepatocellular carcinoma (HCC) cancer stemness. Site-directed mutagenesis of CPE sites in SIRT1 3' UTR confirmed direct CPEB1 targeting. CPEB1 overexpression reduced self-renewal and stemness markers in HCC in vitro and in vivo. Site-directed mutagenesis, luciferase reporter assay with SIRT1 3' UTR, RNA immunoprecipitation, poly(A) tail assay, orthotopic mouse model Cell death & disease High 30237545
2021 Mitotic spindles contain CPE-localized mRNAs and translating ribosomes. CPEB1 and CPEB4 both localize to mitotic spindles and function sequentially: CPEB1 controls metaphase and CPEB4 controls anaphase/cytokinesis by binding specific spindle-associated transcripts and controlling expression and/or localization of their encoded factors. RNA-FISH of spindle-associated mRNAs, immunofluorescence of CPEB1/CPEB4 at spindles, ribosome localization at spindles, RNAi knockdown with cell cycle staging RNA (New York, N.Y.) Medium 33323527
2022 CPEB1 regulates the translational landscape during muscle satellite cell (SC) quiescence-to-activation transition. Phosphorylation-dependent CPEB1 promotes MyoD1 (Myod1) protein synthesis by binding CPEs in the Myod1 3' UTR, driving SC activation and muscle regeneration. In vivo mouse perfusion fixation to isolate bona fide quiescent SCs, quantitative proteomics + transcriptomics, CPEB1 binding to Myod1 3' UTR CPEs, phosphorylation analysis, genetic mouse models of SC activation Nature communications High 35177647
2022 CPEB1 (but not CPEB2-4) forms ribonucleoprotein complexes that are remodeled upon a single phosphorylation event, and these complexes are associated with mRNAs containing canonical CPEs. All four CPEBs can recruit the CCR4-NOT deadenylation complex to repress translation. CPEB2-4 are regulated by multiple proline-directed phosphorylations that control liquid-liquid phase separation, distinguishing them mechanistically from CPEB1. Biochemical fractionation of RNP complexes, RNA-binding assays, phosphorylation analysis, phase separation assays, RNA-seq of target mRNAs Genome biology High 36096799
2003 CPEB phosphorylation occurs at Thr-171 in mouse oocytes at embryonic day E16.5 (pachytene) and is dephosphorylated at E18.5 (diplotene) by the phosphatase PP1; phosphorylation is mediated by Aurora kinase. This temporal regulation of CPEB phosphorylation provides a mechanism for controlled activation of CPE-containing mRNA translation during meiosis. Phospho-specific antibody analysis of mouse oocytes at defined developmental stages, PP1 inhibitor and Aurora kinase inhibitor assays Genes & development Medium 12815066
2006 CPEB controls oocyte growth and follicle development in the mouse. Oocyte-specific CPEB knockdown causes parthenogenetic cell division, abnormal polar bodies, spindle anomalies, follicular apoptosis, and infertility. CPEB binds multiple oocyte mRNAs including Gdf9; in CPEB knockdown oocytes, Gdf9 mRNA has a shortened poly(A) tail and reduced expression. Zp3-promoter driven siRNA transgenic knockdown, poly(A) tail assay for Gdf9, immunofluorescence of spindles, CPEB RNA co-immunoprecipitation Development (Cambridge, England) High 17050619
2008 Translational regulation of beta-catenin mRNA by CPEB1 is required for localization of beta-catenin to the leading edge of migrating astrocytes and for directed cell migration in an in vitro wound-healing assay. Reporter mRNA containing CPEB1-binding sites is transported to the leading edge of migrating glioblastoma cells; point mutations in the binding sites abolish leading-edge localization. In vitro wound-healing assay, dominant-negative CPEB1, CPE reporter mRNA localization with point mutations, immunofluorescence Molecular cancer research: MCR Medium 23360795
2012 PKCε co-immunoprecipitates with CPEB in IB4-positive nociceptors, and CPEB is downstream of PKCε in the signaling cascade responsible for hyperalgesic priming. Antisense knockdown of CPEB prevents but does not reverse psiεRACK (PKCε agonist)-induced priming, establishing CPEB as required for priming induction. Co-immunoprecipitation of PKCε and CPEB, intrathecal antisense oligodeoxynucleotides, behavioral hyperalgesia assay The Journal of neuroscience Medium 22323716
2016 DAZL and CPEB1 synergistically regulate maternal mRNA translation during oocyte meiotic re-entry. Depletion of either DAZL or CPEB1 impairs ribosome loading onto target mRNAs (e.g., Tex19.1). Mutagenesis of DAZL-binding sites and CPEs in target 3' UTRs demonstrates that both proteins cooperate directly on the same mRNA. Genome-wide analysis confirms synergism between DAZL-binding sites and CPEs. Ribosome loading assay (polysome fractionation), oocyte depletion of DAZL and CPEB1, 3' UTR mutagenesis, genome-wide polysome analysis Journal of cell science High 26826184
2023 PATL2, an oocyte-specific RNA-binding protein, physically interacts with both EIF4E and CPEB1 to regulate maternal mRNA expression in immature oocytes. Patl2-/- germinal vesicle oocytes show reduced maternal mRNA expression and decreased protein synthesis. PATL2 phosphorylation at S279 is identified during oocyte maturation and S279D mutation decreases PATL2 protein levels via ubiquitin-mediated proteasomal degradation. Co-immunoprecipitation of PATL2-EIF4E-CPEB1, Patl2 knockout oocytes, phosphoproteomics, knock-in mouse model, ubiquitin-proteasome pathway analysis Development (Cambridge, England) Medium 37218508

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Translational control by CPEB: a means to the end. Nature reviews. Molecular cell biology 485 11433366
2007 CPEB: a life in translation. Trends in biochemical sciences 442 17481902
2003 A neuronal isoform of the aplysia CPEB has prion-like properties. Cell 433 14697205
1998 CPEB-mediated cytoplasmic polyadenylation and the regulation of experience-dependent translation of alpha-CaMKII mRNA at synapses. Neuron 415 9856468
1994 CPEB is a specificity factor that mediates cytoplasmic polyadenylation during Xenopus oocyte maturation. Cell 387 7954828
2003 A neuronal isoform of CPEB regulates local protein synthesis and stabilizes synapse-specific long-term facilitation in aplysia. Cell 324 14697206
2010 Aplysia CPEB can form prion-like multimers in sensory neurons that contribute to long-term facilitation. Cell 286 20144764
2004 Symplekin and xGLD-2 are required for CPEB-mediated cytoplasmic polyadenylation. Cell 272 15550246
2000 CPEB, maskin, and cyclin B1 mRNA at the mitotic apparatus: implications for local translational control of cell division. Cell 238 11081630
1996 CPEB controls the cytoplasmic polyadenylation of cyclin, Cdk2 and c-mos mRNAs and is necessary for oocyte maturation in Xenopus. The EMBO journal 238 8665866
2005 The translational regulator CPEB1 provides a link between dcp1 bodies and stress granules. Journal of cell science 234 15731006
2007 Function of the Drosophila CPEB protein Orb2 in long-term courtship memory. Nature neuroscience 215 17965711
2003 Facilitation of dendritic mRNA transport by CPEB. Genes & development 212 12629046
2002 N-methyl-D-aspartate receptor signaling results in Aurora kinase-catalyzed CPEB phosphorylation and alpha CaMKII mRNA polyadenylation at synapses. The EMBO journal 202 11980711
2000 Phosphorylation of CPEB by Eg2 mediates the recruitment of CPSF into an active cytoplasmic polyadenylation complex. Molecular cell 199 11106762
2001 Germ cell differentiation and synaptonemal complex formation are disrupted in CPEB knockout mice. Developmental cell 184 11702780
2011 CPEB and two poly(A) polymerases control miR-122 stability and p53 mRNA translation. Nature 171 21478871
2002 Differential mRNA translation and meiotic progression require Cdc2-mediated CPEB destruction. The EMBO journal 152 11927567
2007 CPEB interacts with an ovary-specific eIF4E and 4E-T in early Xenopus oocytes. The Journal of biological chemistry 151 17942399
2003 Two previously undescribed members of the mouse CPEB family of genes and their inducible expression in the principal cell layers of the hippocampus. Proceedings of the National Academy of Sciences of the United States of America 151 12871996
1998 Specificity of RNA binding by CPEB: requirement for RNA recognition motifs and a novel zinc finger. Molecular and cellular biology 141 9447964
2004 Selective modulation of some forms of schaffer collateral-CA1 synaptic plasticity in mice with a disruption of the CPEB-1 gene. Learning & memory (Cold Spring Harbor, N.Y.) 136 15169862
2010 Mitotic cell-cycle progression is regulated by CPEB1 and CPEB4-dependent translational control. Nature cell biology 133 20364142
2013 CPEB1 coordinates alternative 3'-UTR formation with translational regulation. Nature 128 23434754
2012 The CPEB-family of proteins, translational control in senescence and cancer. Ageing research reviews 125 22542725
2008 CPEB regulation of human cellular senescence, energy metabolism, and p53 mRNA translation. Genes & development 122 19141477
2000 CPEB proteins control two key steps in spermatogenesis in C. elegans. Genes & development 122 11040214
2012 Generation of a pain memory in the primary afferent nociceptor triggered by PKCε activation of CPEB. The Journal of neuroscience : the official journal of the Society for Neuroscience 116 22323716
2008 Sustained CPEB-dependent local protein synthesis is required to stabilize synaptic growth for persistence of long-term facilitation in Aplysia. Neuron 112 18817739
2006 CPEB controls oocyte growth and follicle development in the mouse. Development (Cambridge, England) 112 17050619
2006 Regulated Pumilio-2 binding controls RINGO/Spy mRNA translation and CPEB activation. Genes & development 108 16418484
2004 Progesterone and insulin stimulation of CPEB-dependent polyadenylation is regulated by Aurora A and glycogen synthase kinase-3. Genes & development 107 14724178
2013 Genetic and acute CPEB1 depletion ameliorate fragile X pathophysiology. Nature medicine 105 24141422
2020 Antagonizing circRNA_002581-miR-122-CPEB1 axis alleviates NASH through restoring PTEN-AMPK-mTOR pathway regulated autophagy. Cell death & disease 96 32054840
2007 RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB. Genes & development 95 17938241
2010 Meiosis requires a translational positive loop where CPEB1 ensues its replacement by CPEB4. The EMBO journal 93 20531391
2001 CPEB phosphorylation and cytoplasmic polyadenylation are catalyzed by the kinase IAK1/Eg2 in maturing mouse oocytes. Development (Cambridge, England) 89 11526086
2004 Cytoplasmic polyadenylation element (CPE)- and CPE-binding protein (CPEB)-independent mechanisms regulate early class maternal mRNA translational activation in Xenopus oocytes. The Journal of biological chemistry 87 14752101
2006 Reduced extinction of hippocampal-dependent memories in CPEB knockout mice. Learning & memory (Cold Spring Harbor, N.Y.) 83 16452649
2012 Drosophila CPEB Orb2A mediates memory independent of Its RNA-binding domain. Neuron 82 23083740
2006 Translational control by neuroguidin, a eukaryotic initiation factor 4E and CPEB binding protein. Molecular and cellular biology 82 16705177
2006 Control of cellular senescence by CPEB. Genes & development 80 17015432
2001 CPEB degradation during Xenopus oocyte maturation requires a PEST domain and the 26S proteasome. Developmental biology 78 11237472
2008 A molecular circuit composed of CPEB-1 and c-Jun controls growth hormone-mediated synaptic plasticity in the mouse hippocampus. The Journal of neuroscience : the official journal of the Society for Neuroscience 77 18716208
2015 Sequential Functions of CPEB1 and CPEB4 Regulate Pathologic Expression of Vascular Endothelial Growth Factor and Angiogenesis in Chronic Liver Disease. Gastroenterology 76 26627607
1998 Characterization of the zebrafish Orb/CPEB-related RNA binding protein and localization of maternal components in the zebrafish oocyte. Mechanisms of development 76 9784598
2016 DAZL and CPEB1 regulate mRNA translation synergistically during oocyte maturation. Journal of cell science 75 26826184
2014 A fly trap mechanism provides sequence-specific RNA recognition by CPEB proteins. Genes & development 75 24990967
2011 Protein-only mechanism induces self-perpetuating changes in the activity of neuronal Aplysia cytoplasmic polyadenylation element binding protein (CPEB). Proceedings of the National Academy of Sciences of the United States of America 71 21270333
2015 CPEB1 mediates epithelial-to-mesenchyme transition and breast cancer metastasis. Oncogene 69 26411364
2012 CPEB-mediated ZO-1 mRNA localization is required for epithelial tight-junction assembly and cell polarity. Nature communications 69 22334078
2005 Dose-dependent control of proliferation and sperm specification by FOG-1/CPEB. Development (Cambridge, England) 69 16000383
2008 The RNA binding protein CPEB regulates dendrite morphogenesis and neuronal circuit assembly in vivo. Proceedings of the National Academy of Sciences of the United States of America 67 19074264
2007 Mechanism of degradation of CPEB during Xenopus oocyte maturation. Proceedings of the National Academy of Sciences of the United States of America 65 17986610
2013 Characterization of prion-like conformational changes of the neuronal isoform of Aplysia CPEB. Nature structural & molecular biology 62 23435382
2015 MicroRNA-22 Gates Long-Term Heterosynaptic Plasticity in Aplysia through Presynaptic Regulation of CPEB and Downstream Targets. Cell reports 61 26095361
2009 Expression of CPEB, GAPDH and U6snRNA in cervical and ovarian tissue during cancer development. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 60 19161537
2007 MAPK interacts with XGef and is required for CPEB activation during meiosis in Xenopus oocytes. Journal of cell science 58 17344432
2014 Acute synthesis of CPEB is required for plasticity of visual avoidance behavior in Xenopus. Cell reports 56 24529705
2009 The nuclear experience of CPEB: implications for RNA processing and translational control. RNA (New York, N.Y.) 56 20040591
1999 Dual roles of p82, the clam CPEB homolog, in cytoplasmic polyadenylation and translational masking. RNA (New York, N.Y.) 56 9917064
2013 CPEB1, a histone-modified hypomethylated gene, is regulated by miR-101 and involved in cell senescence in glioma. Cell death & disease 54 23788032
2001 An autoregulatory feedback loop directs the localized expression of the Drosophila CPEB protein Orb in the developing oocyte. Development (Cambridge, England) 54 11245581
2016 RNA-binding protein CPEB1 remodels host and viral RNA landscapes. Nature structural & molecular biology 50 27775709
2008 Translational control in early development: CPEB, P-bodies and germinal granules. Biochemical Society transactions 50 18631138
2016 RNA-binding profiles of Drosophila CPEB proteins Orb and Orb2. Proceedings of the National Academy of Sciences of the United States of America 49 27791065
2011 CPEB1, a novel gene silenced in gastric cancer: a Drosophila approach. Gut 47 22052064
2003 Regulated CPEB phosphorylation during meiotic progression suggests a mechanism for temporal control of maternal mRNA translation. Genes & development 45 12815066
1999 The clam 3' UTR masking element-binding protein p82 is a member of the CPEB family. RNA (New York, N.Y.) 43 9917063
2012 Identification of a conserved interface between PUF and CPEB proteins. The Journal of biological chemistry 41 22496444
2012 The CPEB protein Orb2 has multiple functions during spermatogenesis in Drosophila melanogaster. PLoS genetics 40 23209437
2021 The role of CPEB family proteins in the nervous system function in the norm and pathology. Cell & bioscience 39 33789753
2018 CPEB1 mediates hepatocellular carcinoma cancer stemness and chemoresistance. Cell death & disease 39 30237545
2008 Nucleocytoplasmic traffic of CPEB1 and accumulation in Crm1 nucleolar bodies. Molecular biology of the cell 39 18923137
2013 CPEB1 regulates the expression of MTDH/AEG-1 and glioblastoma cell migration. Molecular cancer research : MCR 38 23360795
2021 CPEB1 enhances erastin-induced ferroptosis in gastric cancer cells by suppressing twist1 expression. IUBMB life 36 34184391
2008 CPEB1 regulates beta-catenin mRNA translation and cell migration in astrocytes. Glia 36 18618654
2011 CPEB control of NF-kappaB nuclear localization and interleukin-6 production mediates cellular senescence. Molecular and cellular biology 35 21536657
2011 The Drosophila CPEB protein Orb2 has a novel expression pattern and is important for asymmetric cell division and nervous system function. Genetics 35 21900268
2014 CPEB1 modulates differentiation of glioma stem cells via downregulation of HES1 and SIRT1 expression. Oncotarget 34 25216517
2018 MicroRNA-183 induces epithelial-mesenchymal transition and promotes endometrial cancer cell migration and invasion in by targeting CPEB1. Journal of cellular biochemistry 33 29923214
2017 Genetic dissection of the planarian reproductive system through characterization of Schmidtea mediterranea CPEB homologs. Developmental biology 32 28434803
2010 Comparative in silico analyses of cpeb1-4 with functional predictions. Bioinformatics and biology insights 32 20838664
2022 CPEB1 directs muscle stem cell activation by reprogramming the translational landscape. Nature communications 31 35177647
2022 Comparative analyses of vertebrate CPEB proteins define two subfamilies with coordinated yet distinct functions in post-transcriptional gene regulation. Genome biology 30 36096799
2016 Localized Translation of gurken/TGF-α mRNA during Axis Specification Is Controlled by Access to Orb/CPEB on Processing Bodies. Cell reports 30 26947065
2023 CPEB and translational control by cytoplasmic polyadenylation: impact on synaptic plasticity, learning, and memory. Molecular psychiatry 29 37131078
2019 The Drosophila CPEB Protein Orb Specifies Oocyte Fate by a 3'UTR-Dependent Autoregulatory Loop. Genetics 28 31594794
2016 CPEB1 restrains proliferation of Glioblastoma cells through the regulation of p27(Kip1) mRNA translation. Scientific reports 28 27142352
2023 PATL2 regulates mRNA homeostasis in oocytes by interacting with EIF4E and CPEB1. Development (Cambridge, England) 27 37218508
2014 CPEB regulation of TAK1 synthesis mediates cytokine production and the inflammatory immune response. Molecular and cellular biology 27 25452303
2010 Porcine CPEB1 is involved in Cyclin B translation and meiotic resumption in porcine oocytes. Animal science journal = Nihon chikusan Gakkaiho 27 20662813
2021 mRNA spindle localization and mitotic translational regulation by CPEB1 and CPEB4. RNA (New York, N.Y.) 25 33323527
2021 DNA hypermethylation contributes to colorectal cancer metastasis by regulating the binding of CEBPB and TFCP2 to the CPEB1 promoter. Clinical epigenetics 25 33892791
2000 The heme-independent manganese-peroxidase activity depends on the presence of the C-terminal domain within the Streptomyces reticuli catalase-peroxidase CpeB. European journal of biochemistry 25 10806381
2021 MiR-122-5p Mitigates Inflammation, Reactive Oxygen Species and SH-SY5Y Apoptosis by Targeting CPEB1 After Spinal Cord Injury Via the PI3K/AKT Signaling Pathway. Neurochemical research 24 33528808
2016 Spinal CPEB-mtROS-CBP signaling pathway contributes to perineural HIV gp120 with ddC-related neuropathic pain in rats. Experimental neurology 24 27090160
2014 Spermatid cyst polarization in Drosophila depends upon apkc and the CPEB family translational regulator orb2. PLoS genetics 24 24830287
2014 Aurora kinase A is not involved in CPEB1 phosphorylation and cyclin B1 mRNA polyadenylation during meiotic maturation of porcine oocytes. PloS one 23 24983972
2007 Developmental expression of FOG-1/CPEB protein and its control in the Caenorhabditis elegans hermaphrodite germ line. Developmental dynamics : an official publication of the American Association of Anatomists 23 17279572

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