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Showing CCP110 — CP110 is a alias.

CCP110

Centriolar coiled-coil protein of 110 kDa · UniProt O43303

Length
1012 aa
Mass
113.4 kDa
Annotated
2026-06-09
40 papers in source corpus 29 papers cited in narrative 29 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CP110 (CCP110) is a centriolar distal-end capping protein that governs both centriole biogenesis and the licensing of ciliogenesis (PMID:12361598, PMID:19481458). It localizes to the distal end of centrioles where it restrains centriolar microtubule extension, acting antagonistically to the elongation-promoting factor CPAP, such that CP110 loss yields aberrantly elongated microtubule structures (PMID:19481458). CP110 is recruited to centrosomes within a complex with CEP97 (PMID:17719545), and together this complex caps the mother centriole to suppress primary cilium assembly; a separable CP110–CEP290 complex is additionally required for ciliogenesis suppression and couples to Rab8a-dependent membrane trafficking (PMID:17719545, PMID:18694559). Its capping activity is reinforced by interactions with Kif24, MPP9, ENKD1, CCP5/CCP6, and centrin2, which collectively retain CP110 at the mother centriole until ciliogenesis is triggered (PMID:21620453, PMID:25753040, PMID:30375385, PMID:35301795, PMID:37226238). CP110 is required for centriole/centrosome duplication and its activity is controlled by phosphorylation: CDK2 phosphorylation supports duplication and mitotic fidelity, and PLK4 phosphorylation at Ser98 promotes centriole assembly in part by augmenting centrosomal SAS6 (PMID:12361598, PMID:28562169). The timing of cilia assembly is set by regulated CP110 destruction — proteasomal degradation through SCF(Cyclin F) and additional ubiquitin ligases (EDD1/DDB1–VPRBP, LUBAC-generated linear chains read by PRPF8, HERC2 delivered via EHD1-controlled centriolar satellites), p97/VCP-dependent extraction, and selective autophagy via the LIR-containing receptor NudCL2 — counterbalanced by the deubiquitinase USP33 (PMID:20596027, PMID:23486064, PMID:34480124, PMID:34259627, PMID:34813648, PMID:37074924, PMID:39785673). CP110 is essential in vivo: Cp110-null mice die after birth with organogenesis defects, impaired Shh signaling, and basal-body docking failures, establishing CP110's role in subdistal appendage assembly and ciliary vesicle anchoring (PMID:26965371).

Mechanistic history

Synthesis pass · year-by-year structured walk · 17 steps
  1. 2002 High

    Established CP110 as a CDK2 substrate at centrosomes required for centrosome duplication, defining its entry point into the centriole cycle.

    Evidence CDK substrate screen, in vitro kinase assay, and RNAi with centrosome phenotype readout

    PMID:12361598

    Open questions at the time
    • Did not define the structural target of capping
    • Mechanism linking phosphorylation to duplication not resolved
  2. 2006 High

    Showed CP110 assembles into large centrin/calmodulin-containing complexes and functions in late cytokinesis, broadening its role beyond duplication.

    Evidence Yeast two-hybrid, reciprocal Co-IP, in vitro binding, and RNAi/dominant-negative with cytokinesis readout

    PMID:16760425

    Open questions at the time
    • Composition of the megadalton complexes not fully resolved
    • Link between cytokinesis role and centriolar capping unclear
  3. 2007 High

    Identified CEP97 as the recruiter of CP110 to centrosomes and demonstrated the CP110–CEP97 module suppresses ciliogenesis, the central regulatory axis of the gene.

    Evidence Biochemical complex purification, RNAi, dominant-negative, and cilia-formation assays

    PMID:17719545

    Open questions at the time
    • How the cap physically blocks cilium initiation not shown
    • Signals removing the cap unknown at this point
  4. 2008 High

    Resolved a distinct CP110–CEP290 complex required for ciliogenesis suppression and linked CP110 to Rab8a-dependent ciliary membrane trafficking.

    Evidence Reciprocal Co-IP, RNAi epistasis, and ciliogenesis assays

    PMID:18694559

    Open questions at the time
    • How CEP290 and CEP97 complexes are coordinated unclear
    • Direct CP110–Rab8a connection not defined
  5. 2009 High

    Defined CP110 mechanistically as a distal-end cap restraining centriolar microtubule elongation, antagonistic to CPAP.

    Evidence siRNA/overexpression with immunofluorescence and electron microscopy of centriole ultrastructure

    PMID:19481458

    Open questions at the time
    • Molecular basis of microtubule-end capping not structurally defined
    • How antagonism with CPAP is balanced unknown
  6. 2010 High

    Identified SCF(Cyclin F) as the ubiquitin ligase that degrades CP110 in G2, establishing that timed CP110 destruction enforces mitotic fidelity.

    Evidence Interaction screen, Co-IP, in vitro ubiquitination, siRNA, and stable-mutant phenotype

    PMID:20596027

    Open questions at the time
    • Did not address ciliogenesis-coupled degradation
    • Other degradation routes not yet known
  7. 2011 High

    Showed the kinesin-13 Kif24 acts with the CP110–CEP97 cap at mother centrioles to remodel centriolar microtubules and restrain cilia assembly.

    Evidence Co-IP, in vitro microtubule depolymerization, RNAi, and ciliogenesis assay

    PMID:21620453

    Open questions at the time
    • How Kif24 recruitment is regulated not resolved
    • Coupling of depolymerization to capping unclear
  8. 2013 High

    Identified USP33 as the deubiquitinase that stabilizes CP110, defining a ubiquitin/deubiquitin balance controlling centriole number.

    Evidence Co-IP, in vitro deubiquitination with substrate specificity, RNAi, and centrosome-number assay

    PMID:23486064

    Open questions at the time
    • What sets the USP33/Cyclin F balance temporally unknown
    • Role in ciliogenesis-coupled removal not addressed
  9. 2014 High

    Connected CP110 to vertebrate motile ciliogenesis through miR-34/449-mediated repression and to a distal-cap complex with Talpid3, showing CP110 levels gate basal body maturation.

    Evidence miRNA knockout mice, Xenopus and morpholino models, Co-IP, and rescue epistasis

    PMID:24421332 PMID:24899310

    Open questions at the time
    • How transcriptional/miRNA control integrates with degradation unclear
    • Talpid3 ring assembly mechanism not defined
  10. 2015 High

    Placed centrin2 upstream of CP110 removal during ciliogenesis and identified CP110 as a mediator of CDK2-inhibition-driven anaphase catastrophe in cancer cells.

    Evidence CETN2 gene disruption with epistasis rescue; siRNA/overexpression, site-directed mutagenesis (Ser170/Thr194), and live imaging

    PMID:25753040 PMID:25808870 PMID:26304236

    Open questions at the time
    • How centrin2 triggers cap removal mechanistically unknown
    • Anaphase catastrophe link is correlative at the centrosome-clustering level
  11. 2016 High

    Demonstrated CP110 is required in vivo for basal body membrane docking, subdistal appendage assembly, and Shh signaling, and that optimal CP110 levels can promote ciliogenesis context-dependently.

    Evidence Cp110 knockout mouse with multi-tissue ciliogenesis and signaling readouts; Xenopus domain mutagenesis

    PMID:26965371 PMID:27623009

    Open questions at the time
    • Dual inhibitory/promoting roles not mechanistically reconciled
    • Direct CP110 role in SDA assembly versus indirect effect unclear
  12. 2017 Medium

    Identified PLK4 phosphorylation of CP110 at Ser98 as a positive regulator of centriole assembly linked to SAS6 levels.

    Evidence In vitro kinase assay, phospho-mutant overexpression, and SAS6 quantification

    PMID:28562169

    Open questions at the time
    • Single-lab; in vivo relevance of Ser98 not tested in animals
    • Direct effect on cartwheel stabilization inferred, not shown
  13. 2018 High

    Defined a KIF24–MPP9 ring that recruits the CP110–CEP97 cap and showed TTBK2-driven MPP9 degradation licenses cap removal at ciliogenesis onset.

    Evidence Co-IP, direct binding, super-resolution microscopy, siRNA, and TTBK2 kinase assay

    PMID:30375385

    Open questions at the time
    • How TTBK2 activation is timed not resolved
    • Quantitative stoichiometry of the cap assembly unknown
  14. 2021 High

    Expanded CP110 removal mechanisms to selective autophagy (NudCL2/LC3), the CEP78–EDD1 ubiquitin pathway, and LUBAC linear ubiquitination read by PRPF8, showing redundant degradation routes converge on cap clearance.

    Evidence Knockouts with domain/LIR mutagenesis, Co-IP, in vitro ubiquitination, epistasis rescue, and zebrafish models

    PMID:34259627 PMID:34480124 PMID:34813648

    Open questions at the time
    • How proteasomal, autophagic, and linear-ubiquitin routes are prioritized unknown
    • Spatial coordination of multiple ligases at the mother centriole unclear
  15. 2022 Medium

    Revealed additional regulators of CP110 retention/removal (ENKD1 competing with CEP97) and a non-ciliary role in centrosomal aggresome seeding, plus Cdk–Cyclin-entrained crosstalk with proximal centriole assembly.

    Evidence Competitive binding, knockout mice, super-resolution microscopy, aggresome assays, and Drosophila live imaging

    PMID:35301795 PMID:35411088 PMID:35707992

    Open questions at the time
    • Aggresome role mechanistically separable from capping unclear
    • Distal-to-proximal crosstalk mechanism not molecularly defined
  16. 2023 Medium

    Identified satellite-delivered HERC2 (via EHD1) and MIB1 as CP110 ligases, CCP5/CCP6 as enzymatic-activity-independent retention factors, and ODF2 as a scaffold influencing CP110 levels.

    Evidence Co-IP/CoIP-MS, ubiquitination assays, RNAi, satellite trafficking assays, and dimerization recruitment

    PMID:37074924 PMID:37226238 PMID:37681926

    Open questions at the time
    • ODF2 scaffold role not directly reconstituted (Low confidence)
    • How many parallel ligases are physiologically rate-limiting unknown
  17. 2025 Medium

    Established p97/VCP as the AAA-ATPase that extracts polyubiquitinated CP110 from the mother centriole and linked CP110/CEP97 removal to EHD1-driven tubular ciliary membrane intermediates.

    Evidence siRNA/chemical inhibition with CP110 epistasis; 3D electron microscopy of membrane intermediates (preprint)

    PMID:39785673

    Open questions at the time
    • Direct demonstration of p97 unfolding CP110 not shown
    • Coupling of membrane tubulation to ubiquitin-dependent extraction not resolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the cell integrates multiple parallel CP110-removal pathways (proteasome, autophagy, p97 extraction) with membrane remodeling and the diverse retention factors into a single deterministic ciliogenesis switch remains unresolved.
  • No unified model of pathway hierarchy or redundancy
  • Structural basis of CP110 microtubule-end capping unknown
  • Quantitative dynamics of cap assembly/disassembly at the mother centriole undefined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 2 GO:0098772 molecular function regulator activity 2
Localization
GO:0005815 microtubule organizing center 3 GO:0005856 cytoskeleton 2
Pathway
R-HSA-392499 Metabolism of proteins 4 R-HSA-1852241 Organelle biogenesis and maintenance 3 R-HSA-1640170 Cell Cycle 2
Partners
CEP290CEP97CETN2CYCLIN FENKD1KIF24MPP9USP33
Complex memberships
CP110-CEP290 complexCP110-CEP97 complex

Evidence

Reading pass · 29 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2002 CP110 is phosphorylated by CDKs (CDK2) in vitro and in vivo, localizes to centrosomes, and is required for centrosome duplication; RNAi-mediated depletion inhibits centrosome duplication, and long-term disruption of CP110 phosphorylation leads to unscheduled centrosome separation and polyploidy. CDK substrate screen, in vitro kinase assay, immunofluorescence localization, RNAi knockdown with centrosome phenotype readout Developmental cell High 12361598
2006 CP110 directly interacts with calmodulin (CaM) and centrin in vivo; CP110 exists in large complexes (~300 kDa to 3 MDa) containing both centrin and CaM; CP110 depletion or expression of a CaM-binding-deficient CP110 mutant causes failure at a late stage of cytokinesis and binucleate cell formation. Yeast two-hybrid, co-immunoprecipitation, in vitro binding assay, RNAi knockdown, expression of dominant-negative mutant Molecular biology of the cell High 16760425
2007 CP110 forms a complex with Cep97, which recruits CP110 to centrosomes; depletion of Cep97 causes CP110 disappearance from centrosomes, spindle defects, and polyploidy. Loss of Cep97 or CP110 promotes primary cilia formation in growing cells, and enforced CP110 expression in quiescent cells suppresses cilia assembly, demonstrating that Cep97 and CP110 collaborate to inhibit ciliogenesis. Biochemical complex purification, RNAi knockdown, dominant-negative expression, immunofluorescence, cilia formation assay Cell High 17719545
2008 CP110 interacts with CEP290 in a discrete complex separable from other CP110 complexes; interaction with CEP290 is required for CP110's ability to suppress primary cilia formation. CEP290 and CP110 also interact with Rab8a; depletion of CEP290 prevents ciliogenesis and mislocalizes Rab8a at centrosomes and cilia. Co-immunoprecipitation, RNAi knockdown, immunofluorescence, ciliogenesis assay Developmental cell High 18694559
2009 CP110 acts as a distal end-capping protein of centrioles; depletion of CP110 leads to elongated microtubule structures extending from centrioles. CP110 and CPAP play antagonistic roles in controlling the length of newly formed centrioles, with CPAP promoting tubulin addition and CP110 restraining it. siRNA knockdown, overexpression, immunofluorescence, electron microscopy of centriole ultrastructure Current biology : CB High 19481458
2010 Cyclin F (Fbxo1) is the substrate recognition subunit of SCF(Cyclin F) ubiquitin ligase complex that physically associates with CP110 at centrioles during G2 and ubiquitylates CP110, leading to its proteasomal degradation. SCF(Cyclin F)-mediated CP110 degradation is required for mitotic fidelity; stable CP110 causes multipolar spindles and chromosome instability. Unbiased protein interaction screen, co-immunoprecipitation, ubiquitination assay, siRNA knockdown, expression of stable CP110 mutant, cell biology phenotype readouts Nature High 20596027
2011 Kif24, a kinesin-13 subfamily motor protein, specifically interacts with CP110 and Cep97 at mother centrioles; loss of Kif24 causes disappearance of CP110 from mother centrioles in cycling cells and leads to aberrant cilia assembly. Kif24 depolymerizes microtubules in vitro and specifically remodels centriolar microtubules in cells. Co-immunoprecipitation, RNAi knockdown, in vitro microtubule depolymerization assay, immunofluorescence, ciliogenesis assay Cell High 21620453
2012 In Drosophila, CP110 depletion results in centriole length diminution (in contrast to mammalian cells where depletion causes elongation); co-depletion of CP110 and Klp10A (kinesin-13) gives longer centrioles than usual, indicating functional interaction between these two proteins in controlling centriole length. RNAi in Drosophila cultured cells and testes, electron microscopy of centriole ultrastructure, co-depletion epistasis Current biology : CB Medium 22365849
2013 USP33, a deubiquitinating enzyme, interacts with CP110 and localizes to centrioles primarily in S and G2/M phases; USP33 potently and specifically deubiquitinates CP110 (but not other cyclin-F substrates), counteracting SCF(cyclin F)-mediated ubiquitination. USP33 overactivity promotes supernumerary centrioles; USP33 ablation destabilizes CP110 and inhibits centrosome amplification. Co-immunoprecipitation, in vitro deubiquitination assay, RNAi knockdown, overexpression, centrosome number assay Nature High 23486064
2013 In Drosophila, CP110 subtly influences centriole length by counteracting centriole-elongating activity of duplication proteins; CP110 ensures centriolar microtubules do not extend beyond the distal centriole end; CP110 suppresses centriole overduplication induced by overexpression of duplication proteins. CP110 null mutant fly analysis, overexpression, immunofluorescence and electron microscopy of centrioles The Journal of cell biology Medium 24297749
2014 Talpid3/KIAA0586 is a component of a CP110-containing protein complex; Talpid3 assembles a ring-like structure at the extreme distal end of centrioles. Depletion of Talpid3 causes abnormal centriolar satellite distribution and cilia assembly defects reminiscent of Cep290 loss, including mislocalization of Rab8a; activated Rab8a suppresses cilia defects caused by Talpid3 depletion. Co-immunoprecipitation, RNAi, immunofluorescence, activated GTPase rescue epistasis The Journal of cell biology Medium 24421332
2014 miR-34/449 miRNAs promote motile ciliogenesis in part by post-transcriptionally repressing Cp110; cp110 knockdown in miR-34/449-deficient multiciliated cells (mouse and Xenopus) restored ciliogenesis by rescuing basal body maturation and docking. miRNA knockout mice, Xenopus model, morpholino knockdown, cp110 knockdown rescue epistasis, basal body imaging Nature High 24899310
2015 Centrin2 controls CP110 removal from the mother centriole during ciliogenesis; CETN2-deficient human RPE cells fail to remove CP110 and show abnormal distal appendage protein localization; knockdown of CP110 in CETN2-deficient cells rescues ciliation, placing centrin2 upstream of CP110 removal. CETN2 gene disruption (reverse genetics), immunofluorescence, CP110 knockdown epistasis, ciliogenesis assay The Journal of cell biology High 25753040
2015 CP110 is a critical mediator of CDK2 inhibition-driven anaphase catastrophe; siRNA-mediated repression of CP110 induced anaphase catastrophe in lung cancer cells, while CP110 overexpression antagonized CDK2 inhibitor-mediated anaphase catastrophe. Site-directed mutagenesis identified CDK phosphorylation sites Ser170 and Thr194 as critical for conferring anaphase catastrophe by altering centrosome clustering in mitosis. siRNA knockdown, overexpression, site-directed mutagenesis, live-cell imaging, CDK2 inhibitor treatment Cancer research Medium 25808870 26304236
2016 CP110 is required for anchoring basal bodies to the membrane during cilia formation in vivo; Cp110-/- mice die shortly after birth with organogenesis defects including impaired Shh signaling and reduced primary cilia. CP110 loss results in abnormal distribution of subdistal appendage (SDA) core components and recycling endosomes, implicating CP110 in SDA assembly and ciliary vesicle docking. Cp110 knockout mouse, immunofluorescence, ciliogenesis assay in multiple tissues, Shh signaling readout Development (Cambridge, England) High 26965371
2016 In Xenopus multiciliated cells, Cp110 at optimal levels promotes ciliogenesis by localizing to cilia-forming basal bodies and rootlets and being required for ciliary adhesion complexes that facilitate actin interactions; coiled-coil domains mediate preferential binding to centrioles over rootlets. Cp110 levels are precisely controlled by both ciliary transcription factors and miRNAs. Xenopus in vivo experiments, domain mutagenesis, immunofluorescence, knockdown/overexpression of transcription factors and miRNAs eLife Medium 27623009
2017 PLK4 specifically phosphorylates CP110 at Ser98; phospho-resistant CP110 (S98A) inhibits centriole assembly while phospho-mimetic CP110 (S98D) induces centriole assembly even under PLK4-limiting conditions. Phosphorylated CP110 augments centrosomal SAS6 levels, suggesting involvement in cartwheel stabilization. In vitro kinase assay, site-directed mutagenesis, overexpression of phospho-mutants, immunofluorescence, SAS6 quantification Cell cycle (Georgetown, Tex.) Medium 28562169
2018 MPP9 is recruited by KIF24 to the distal end of the mother centriole where it forms a ring-like structure that recruits the CP110-CEP97 complex by directly binding CEP97. Phosphorylation of MPP9 by TTBK2 at the onset of ciliogenesis targets MPP9 for ubiquitin-proteasome degradation, which facilitates removal of CP110 and CEP97 from the mother centriole. Co-immunoprecipitation, immunofluorescence, siRNA knockdown, super-resolution microscopy, TTBK2 kinase assay Nature communications High 30375385
2021 NudCL2 acts as a selective autophagy receptor at mother centrioles, containing an LIR motif that mediates association of CP110 with the autophagosome marker LC3. Knockout of NudCL2 induces defective CP110 removal from mother centrioles and ciliogenesis defects rescued by wild-type NudCL2 but not its LIR mutant; CP110 knockdown attenuates ciliogenesis defects in NudCL2-deficient cells. NudCL2 knockout, LIR motif mutagenesis, Co-IP, autophagy/LC3 interaction assay, RNAi epistasis, zebrafish morphant analysis Cell research High 34480124
2021 CEP78 promotes ciliogenesis by negatively regulating CP110 levels via the EDD1-DYRK2-DDB1VPRBP E3 ubiquitin ligase complex involved in CP110 ubiquitination and degradation. CEP78 interacts with CEP350, which promotes centrosomal recruitment and stability of CEP78, leading to EDD1 centrosomal recruitment. Cells lacking CEP78 display significantly increased CP110 levels, and depletion of CP110 in CEP78-deficient cells restores ciliation frequency. Disease-based interactome screen, Co-IP, CEP78 knockout, RNAi epistasis, ciliogenesis assay eLife High 34259627
2021 LUBAC (linear ubiquitin chain assembly complex) specifically generates linear ubiquitin chains on CP110, which is required for CP110 removal from the mother centriole during ciliogenesis. PRPF8, located at the distal end of the mother centriole, acts as a receptor for linear ubiquitin chains on CP110 to facilitate its removal at the initial stage of ciliogenesis. Co-immunoprecipitation, in vitro ubiquitination assay, siRNA knockdown, immunofluorescence, ciliogenesis assay The Journal of cell biology High 34813648
2022 Aggresome assembly at the centrosome depends on CP110, CEP97, and CEP290; the seeding of a phosphorylated HSP27 ring around centrioles (the initial aggresome structure) requires these proteins. In senescent cells, reduced CP110 levels impair aggresome formation. siRNA knockdown, quantitative immunofluorescence, aggresome formation assay, mutant huntingtin aggregation assay Nature cell biology Medium 35411088
2022 CP110 and Cep97 form a complex at the distal end of centrioles in Drosophila embryos whose levels rise and fall entrained by the Cdk-Cyclin oscillator; altering CP110 and Cep97 levels changes Plk4 oscillation and cartwheel growth at the proximal end, revealing unexpected crosstalk between distal-end factors and proximal centriole assembly. Live imaging in Drosophila embryos, quantitative fluorescence analysis, overexpression and knockdown of CP110/Cep97 Journal of cell science Medium 35707992
2022 ENKD1 competes with CEP97 for binding to CP110; depletion of ENKD1 enhances the CP110-CEP97 interaction and retains CP110 at the mother centriole. Co-knockdown of ENKD1 and CP110 reverses ciliogenesis defects caused by ENKD1 depletion. Enkd1 knockout mice have ciliogenesis defects in multiple organs. Co-immunoprecipitation, competitive binding assay, siRNA knockdown, knockout mice, super-resolution microscopy, ciliogenesis assay EMBO reports High 35301795
2023 EHD1 regulates CP110 ubiquitination during ciliogenesis; EHD1 controls centriolar satellite movement to the mother centriole, delivering the E3 ubiquitin ligase HERC2, which interacts with and ubiquitinates CP110. MIB1 also ubiquitinates CP110. HERC2 is required for ciliogenesis and localizes to centriolar satellites. Co-immunoprecipitation, ubiquitination assay, siRNA knockdown, immunofluorescence, centriolar satellite trafficking assay EMBO reports High 37074924
2023 CCP5 and CCP6 interact with CP110 (CCP5 through its N-terminus) and retain CP110 at the mother centriole to suppress cilia formation in cycling cells. Loss of CCP5 or CCP6 causes disappearance of CP110 from the mother centriole and increased ciliation; these effects are independent of their enzymatic deglutamylation activity. CoIP-MS, co-immunoprecipitation, RNAi knockdown, immunofluorescence, ciliogenesis assay, domain mapping BMC biology Medium 37226238
2023 ODF2 controls CP110 levels at centrioles; ODF2 knockdown leads to decreased CP110 levels, while ODF2 most likely acts as a scaffold for NEURL4 or HYLS1 binding to mediate CP110 degradation via the ubiquitin-dependent proteasome pathway. siRNA knockdown, rapamycin-mediated dimerization recruitment assay, immunofluorescence, ciliogenesis assay Cells Low 37681926
2025 p97/VCP (valosin-containing protein), an AAA-ATPase, is responsible for removal of CP110 from the mother centriole; p97 knockdown or inhibition impairs ciliogenesis in a mechanism dependent on CP110. p97 is proposed to unfold and extract polyubiquitinated CP110 from the mother centriole. siRNA knockdown, p97 chemical inhibition, ciliogenesis assay, immunofluorescence, epistasis with CP110 Molecular biology of the cell Medium 39785673
2025 EHD1 promotes CP110/CEP97 removal from the mother centriole cap through its membrane tubulation function during formation of tubular ciliogenesis intermediates; EHD1 and RAB8 orchestrate formation of tubular C-shaped and toroidal membrane intermediates upstream of the ciliary vesicle. 3D electron microscopy (isotropic ultrastructure imaging), EHD1 depletion/inhibition, immunofluorescence, quantitative analysis of membrane structures bioRxivpreprint Medium

Source papers

Stage 0 corpus · 40 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2007 Cep97 and CP110 suppress a cilia assembly program. Cell 388 17719545
2009 Control of centriole length by CPAP and CP110. Current biology : CB 282 19481458
2002 CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells. Developmental cell 258 12361598
2010 SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation. Nature 229 20596027
2008 CP110 suppresses primary cilia formation through its interaction with CEP290, a protein deficient in human ciliary disease. Developmental cell 220 18694559
2014 miR-34/449 miRNAs are required for motile ciliogenesis by repressing cp110. Nature 182 24899310
2011 Centriolar kinesin Kif24 interacts with CP110 to remodel microtubules and regulate ciliogenesis. Cell 165 21620453
2012 miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics. Nature cell biology 132 22684256
2013 USP33 regulates centrosome biogenesis via deubiquitination of the centriolar protein CP110. Nature 111 23486064
2014 The CP110-interacting proteins Talpid3 and Cep290 play overlapping and distinct roles in cilia assembly. The Journal of cell biology 98 24421332
2006 CP110 cooperates with two calcium-binding proteins to regulate cytokinesis and genome stability. Molecular biology of the cell 80 16760425
2013 CP110 and its network of partners coordinately regulate cilia assembly. Cilia 70 24053599
2018 M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole. Nature communications 68 30375385
2016 Centrosomal protein CP110 controls maturation of the mother centriole during cilia biogenesis. Development (Cambridge, England) 68 26965371
2016 Ciliary transcription factors and miRNAs precisely regulate Cp110 levels required for ciliary adhesions and ciliogenesis. eLife 61 27623009
2015 Centrin2 regulates CP110 removal in primary cilium formation. The Journal of cell biology 61 25753040
2012 Klp10A, a microtubule-depolymerizing kinesin-13, cooperates with CP110 to control Drosophila centriole length. Current biology : CB 52 22365849
2013 CP110 exhibits novel regulatory activities during centriole assembly in Drosophila. The Journal of cell biology 47 24297749
2015 CDK2 Inhibition Causes Anaphase Catastrophe in Lung Cancer through the Centrosomal Protein CP110. Cancer research 42 25808870
2015 MiR-129-3p promotes docetaxel resistance of breast cancer cells via CP110 inhibition. Scientific reports 40 26487539
2022 Aggresome assembly at the centrosome is driven by CP110-CEP97-CEP290 and centriolar satellites. Nature cell biology 36 35411088
2021 CEP78 functions downstream of CEP350 to control biogenesis of primary cilia by negatively regulating CP110 levels. eLife 33 34259627
2022 ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis. EMBO reports 26 35301795
2021 NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis. Cell research 23 34480124
2021 LUBAC regulates ciliogenesis by promoting CP110 removal from the mother centriole. The Journal of cell biology 21 34813648
2016 miR-129-3p controls centrosome number in metastatic prostate cancer cells by repressing CP110. Oncotarget 21 26918338
2017 PLK4 phosphorylation of CP110 is required for efficient centriole assembly. Cell cycle (Georgetown, Tex.) 20 28562169
2015 Specific CP110 Phosphorylation Sites Mediate Anaphase Catastrophe after CDK2 Inhibition: Evidence for Cooperation with USP33 Knockdown. Molecular cancer therapeutics 20 26304236
2023 EHD1 promotes CP110 ubiquitination by centriolar satellite delivery of HERC2 to the mother centriole. EMBO reports 16 37074924
2024 Emerging insights into CP110 removal during early steps of ciliogenesis. Journal of cell science 13 38415788
2022 Centriole distal-end proteins CP110 and Cep97 influence centriole cartwheel growth at the proximal end. Journal of cell science 11 35707992
2023 lncRNA XIST/miR‑129‑2‑3p axis targets CCP110 to regulate the proliferation, invasion and migration of endometrial cancer cells. Experimental and therapeutic medicine 9 36911384
2023 ODF2 Negatively Regulates CP110 Levels at the Centrioles/Basal Bodies to Control the Biogenesis of Primary Cilia. Cells 7 37681926
2023 CCP5 and CCP6 retain CP110 and negatively regulate ciliogenesis. BMC biology 6 37226238
2023 CP110 and CEP135 localize near the proximal and distal centrioles of cattle and human spermatozoa. microPublication biology 5 37822686
2013 Cell biology: DUBing CP110 controls centrosome numbers. Current biology : CB 4 23701692
2024 CP110 and CEP135 Localize Near the Proximal Centriolar Remnants of Mice Spermatozoa. microPublication biology 3 38351906
2025 Valosin-containing protein p97 extracts capping protein CP110 from the mother centriole to promote ciliogenesis. Molecular biology of the cell 2 39785673
2024 Biallelic loss-of-function variants in the centriolar protein CCP110 leads to a ciliopathy-like phenotype. European journal of medical genetics 1 38857829
2025 BICD2 promotes ciliogenesis by facilitating CP110 removal from the mother centriole. EMBO reports 0 41102520

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