Affinage

CORO1A

Coronin-1A · UniProt P31146

Length
461 aa
Mass
51.0 kDa
Annotated
2026-06-09
70 papers in source corpus 30 papers cited in narrative 31 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CORO1A (coronin 1) is a leukocyte-enriched, seven-bladed WD40 beta-propeller protein that functions as a signaling scaffold coupling membrane receptor activation to Ca2+ and cAMP signaling, cytoskeletal regulation, and the control of organelle fusion across immune cells and neurons (PMID:16407068, PMID:18345003, PMID:24270184). The protein homo-oligomerizes through a C-terminal coiled-coil/leucine zipper, associates with the plasma membrane via its WD domain, and binds the actin cytoskeleton through a positively charged linker; this actin association is negatively regulated by Thr-412 phosphorylation carried out specifically by PKCalpha (PMID:15800061, PMID:15601263, PMID:23100250, PMID:34189278). In macrophages, coronin 1 is recruited to mycobacterial phagosomes where it activates the Ca2+-dependent phosphatase calcineurin to block phagosome-lysosome fusion and protect pathogenic mycobacteria, an activity that depends on its trimeric state and is relieved by serine-phosphorylation-induced monomerization (PMID:17632055, PMID:18162581, PMID:25217836). In T cells, coronin 1 acts downstream of TCR triggering to generate IP3 from PIP2 and mobilize intracellular Ca2+ for proliferation, IL-2 production, and survival, while independently sustaining pro-survival signaling by maintaining basal PI3Kdelta activity that suppresses caspase-8-mediated apoptosis and by restraining cAMP through PDE4 to permit alloreactive T cell responses (PMID:18345003, PMID:34932374, PMID:30611611). Through a CDK5-phosphorylation-dependent interaction with Galphas, coronin 1 activates the cAMP/PKA pathway underlying presynaptic plasticity and behavior (PMID:24667537, PMID:26823173). In neurons it serves as an effector of the NGF-TrkA signaling endosome, preventing premature lysosomal fusion and enabling recycling, calcineurin activation, and CREB phosphorylation, and it suppresses axon overgrowth via PLC-gamma1/Ca2+-dependent inhibition of PI3K-GSK3beta signaling and collaborates with the E3 ligase TRIM67 downstream of netrin-1 to direct axon turning and branching (PMID:24270184, PMID:25740518, PMID:41085995). A C-terminal frameshift mutation (S401fs) that abolishes oligomerization causes defective thymic output and impaired T cell survival in patients, linking C-terminal integrity to human T cell homeostasis (PMID:26476480).

Mechanistic history

Synthesis pass · year-by-year structured walk · 25 steps
  1. 2004 Medium

    Establishing where coronin 1 acts in T cells: it was unknown whether the protein was positioned at sites of actin remodeling during T cell activation.

    Evidence Subcellular fractionation and confocal imaging of TCR-stimulated T lymphocytes

    PMID:14734608

    Open questions at the time
    • Localization alone did not establish a functional requirement
    • No molecular partners at the protrusion site identified
  2. 2005 High

    Defining the molecular architecture: the question was how coronin 1 oligomerizes and engages membrane versus cytoskeleton, resolved by mapping distinct domains for trimerization, membrane, and actin association.

    Evidence Domain-deletion mutagenesis, leucine-zipper mutations, co-IP, fractionation, and biochemical reconstitution in COS-1 cells

    PMID:15601263 PMID:15800061

    Open questions at the time
    • Trimer versus dimer stoichiometry described differently across studies
    • Functional consequence of each domain interaction not yet tested in vivo
  3. 2006 High

    Determining the three-dimensional fold: the structural basis for WD40 propeller assembly and putative F-actin contacts was unknown until a high-resolution crystal structure resolved the seven-bladed propeller and stabilizing C-terminal extension.

    Evidence X-ray crystallography of murine coronin 1 at 1.75 A

    PMID:16407068

    Open questions at the time
    • Structure lacked the leucine zipper
    • Predicted F-actin binding surface not validated functionally
  4. 2006 High

    Testing the cytoskeletal role in vivo: whether coronin 1 governs actin dynamics and migration was resolved by showing it inhibits steady-state F-actin via Arp2/3 and is required for chemokine-driven migration but not TCR function.

    Evidence Coronin 1 knockout mice with F-actin quantification, chemotaxis, and TCR assays

    PMID:16902139

    Open questions at the time
    • Mechanism of Arp2/3-dependent inhibition not detailed
    • Apparent dispensability for TCR later revised
  5. 2007 High

    Identifying the pathogen-protective mechanism: it was unknown how coronin 1 blocks mycobacterial killing, answered by demonstrating it activates calcineurin to prevent phagosome-lysosome fusion.

    Evidence Coronin 1-deficient macrophages with calcineurin assays, cyclosporin A/FK506 inhibition, and fusion assays

    PMID:17632055

    Open questions at the time
    • How phagosomal coronin 1 triggers Ca2+/calcineurin not fully resolved
    • Link between membrane recruitment and calcineurin activation incomplete
  6. 2007 Medium

    Identifying a bacterial ligand: the basis for coronin 1 retention on the mycobacterial phagosome was addressed by identifying LpdC as a cholesterol-dependent binding partner that prolongs intracellular survival.

    Evidence GST-coronin 1 pulldown with mass spectrometry, cell-free binding, and M. smegmatis LpdC gain-of-function

    PMID:17652161

    Open questions at the time
    • Single lab biochemical interaction without genetic confirmation in pathogenic mycobacteria
    • Cholesterol-dependence mechanism not structurally defined
  7. 2008 High

    Separating actin and pathogen functions: RNAi showed coronin 1 is dispensable for phagocytosis, macropinocytosis, locomotion, and oxidase activity but essential to keep mycobacteria from lysosomal killing, isolating its phagosome-regulatory role.

    Evidence siRNA knockdown in J774 macrophages with multiple actin-process and bacterial-killing readouts

    PMID:18162581

    Open questions at the time
    • Does not address coronin 1 actin roles in other phagocyte subtypes
  8. 2008 High

    Placing coronin 1 in TCR signaling: the prior view that it was dispensable for TCR function was revised by showing it is required to generate IP3 and mobilize Ca2+ for T cell proliferation and survival.

    Evidence Coronin 1 KO mice with IP3 measurement, Ca2+ mobilization, and T cell functional assays

    PMID:18345003

    Open questions at the time
    • Direct biochemical step at which coronin 1 enables IP3 generation unresolved
    • Whether the effect is through PLC recruitment or another route unclear
  9. 2008 High

    Defining phosphoregulation of actin binding: PKC-mediated phosphorylation was shown to lower coronin 1 affinity for F-actin and drive its dissociation during phagocytosis.

    Evidence Fractionation, 2D gels, F-actin co-sedimentation, and affinity chromatography in HL60 cells

    PMID:18693254

    Open questions at the time
    • Specific phosphosite not yet identified at this stage
    • Responsible PKC isoform not yet defined
  10. 2009 Medium

    Defining the anti-apoptotic pathway: coronin 1 was shown to protect against the intrinsic mitochondrial apoptotic pathway rather than the extrinsic pathway.

    Evidence Coronin 1 overexpression in PLB985 cells with mitochondrial depolarization and caspase-3/-8/-9 assays

    PMID:19454722

    Open questions at the time
    • Overexpression-based; molecular link to mitochondria not defined
    • Apparent contrast with later caspase-8 findings in T cells unresolved
  11. 2009 Medium

    Extending Ca2+ function to B cells: coronin 1 was shown to be required for BCR-induced Ca2+ mobilization and proliferation but bypassable by costimulation, explaining intact in vivo B cell responses.

    Evidence Coronin 1 KO mice with Ca2+ mobilization, proliferation, and immunization assays

    PMID:19201848

    Open questions at the time
    • Molecular step in BCR-to-Ca2+ coupling not defined
    • Costimulatory bypass mechanism unidentified
  12. 2011 Medium

    Revising the death model in T cells: it was shown F-actin levels are not altered in naive T cells and do not drive apoptosis; instead coronin 1 provides distinct pro-survival signals.

    Evidence Coronin 1 KO mice with F-actin quantification, apoptosis assays, and pharmacological actin manipulation

    PMID:21339362

    Open questions at the time
    • The pro-survival signal itself not molecularly identified here
    • Single lab refutation of prior model
  13. 2012 High

    Pinpointing the regulatory phosphosite: Thr-412 phosphorylation was identified as the switch controlling coronin 1-actin interaction.

    Evidence MALDI-TOF-MS, Phos-tag gels, T412D/T412A mutagenesis, and beta-actin co-IP in HL60 cells

    PMID:23100250

    Open questions at the time
    • Kinase responsible not yet assigned in this study
    • Effect on oligomerization versus actin binding not separated
  14. 2013 High

    Establishing a neuronal endosome function: coronin 1 was shown to be an NGF-TrkA signaling endosome effector that prevents premature lysosomal fusion and enables Ca2+/calcineurin/CREB signaling.

    Evidence Coro1a KO sympathetic neurons with live imaging, fusion kinetics, calcium imaging, and CREB Western blot

    PMID:24270184

    Open questions at the time
    • Molecular mechanism delaying lysosomal fusion unresolved
    • How coronin 1 is recruited to the signaling endosome unclear
  15. 2014 High

    Linking coronin 1 to cAMP/PKA: a Galphas interaction was shown to activate cAMP/PKA signaling required for presynaptic plasticity and behavior, rescuable by cAMP analogues in vivo.

    Evidence Reciprocal co-IP, cAMP measurement, amygdala electrophysiology, behavior, and in vivo cAMP rescue in Coro1a KO mice

    PMID:24667537

    Open questions at the time
    • Stimulus that triggers coronin 1-Galphas coupling not fully defined
    • Relationship to its actin/Ca2+ roles unclear
  16. 2014 Medium

    Connecting oligomeric state to pathogen protection: trimerization was shown to be essential for calcineurin activation and mycobacterial protection, with macrophage-induced serine phosphorylation causing monomerization that relieves protection.

    Evidence Trimerization-defective mutants in macrophages with calcineurin and survival assays

    PMID:25217836

    Open questions at the time
    • Specific monomerizing phosphosite not identified
    • Kinase driving monomerization unknown
  17. 2015 Medium

    Defining cytokine-induced relocalization machinery: phospho-coronin 1 was shown to bind 14-3-3zeta, which drives its cortex-to-cytoplasm relocation, PI3K activation, and macropinocytosis.

    Evidence Co-IP of phospho-coronin 1 with 14-3-3zeta/RACK1, siRNA knockdown, and macropinocytosis assays

    PMID:25645340

    Open questions at the time
    • Single lab Co-IP-based interaction
    • How 14-3-3zeta binding couples to PI3K activation unresolved
  18. 2015 Medium

    Defining the axon-suppression pathway: coronin 1 was shown to restrain axon growth and branching by PLC-gamma1/Ca2+-dependent inhibition of PI3K-GSK3beta signaling, with KO causing cardiac sympathetic axon overgrowth.

    Evidence Coro1a KO mice with in vivo axon morphology and PLC-gamma1/PI3K/GSK3beta epistasis plus calcium imaging

    PMID:25740518

    Open questions at the time
    • Direct molecular target of coronin 1 in this cascade unidentified
    • Single lab pathway placement
  19. 2016 Medium

    Assigning the kinase for Galphas coupling: CDK5 was shown to phosphorylate coronin 1 at Thr418/424, a modification required for Galphas association and cAMP/PKA activation.

    Evidence Phosphosite mutagenesis, CDK5 modulation, coronin 1-Galphas co-IP, and cAMP measurement

    PMID:26823173

    Open questions at the time
    • Structural basis of the phospho-dependent Galphas interface unknown
    • Single lab
  20. 2015 Medium

    Connecting domain integrity to human disease: a C-terminal frameshift (S401fs) was shown to abolish oligomerization and cytoskeletal association, producing defective thymic output and impaired T cell survival in patients.

    Evidence Whole-genome sequencing, oligomerization and fractionation assays, and patient T cell phenotyping

    PMID:26476480

    Open questions at the time
    • Single kindred
    • Normal Ca2+ flux despite oligomerization loss not fully reconciled with prior models
  21. 2019 High

    Refining cAMP control in T cells: coronin 1 deficiency was shown to raise cAMP by relieving PDE4-mediated degradation, suppressing alloreactive T cell responses while sparing anti-pathogen immunity.

    Evidence Coronin 1 KO mice with cAMP measurement, PDE4 pharmacology, allograft, and T cell transfer models

    PMID:30611611

    Open questions at the time
    • How coronin 1 regulates PDE4 activity mechanistically unresolved
    • Reconciliation with Galphas-mediated cAMP activation in neurons unclear
  22. 2021 Medium

    Defining a TCR-independent survival mechanism: coronin 1 was shown to maintain basal PI3Kdelta activity that suppresses caspase-8-mediated apoptosis independently of TCR and IL-7.

    Evidence Coronin 1 KO mice with PI3Kdelta and caspase-8 assays plus genetic/pharmacological epistasis

    PMID:34932374

    Open questions at the time
    • How coronin 1 sustains basal PI3Kdelta unknown
    • Relationship to the earlier intrinsic-apoptosis finding unresolved
  23. 2021 High

    Assigning the Thr-412 kinase in phagocytes: PKCalpha (not PKCbeta) was shown to phosphorylate Thr-412, controlling coronin 1 redistribution from phagosomes.

    Evidence Isoform-specific inhibitors and siRNA, in vitro kinase assays, and confocal localization in human phagocytes

    PMID:34189278

    Open questions at the time
    • Upstream activation of PKCalpha during phagocytosis not defined
    • Single lab
  24. 2025 Medium

    Extending the neuronal role to guidance: coronin 1 was shown to act with the E3 ligase TRIM67 downstream of netrin-1/DCC for axon turning, branching, and corpus callosum development.

    Evidence Coro1a KO mice, growth cone imaging, TRIM67-binding-deficient rescue, and in vivo corpus callosum analysis

    PMID:41085995

    Open questions at the time
    • Whether coronin 1 is a TRIM67 ubiquitination substrate unresolved
    • Single lab
  25. 2026 Medium

    Defining transcriptional control of CORO1A: combined core-promoter Sp3/GC-box and intronic ETS elements were shown to drive expression, with active histone marks declining during osteoclast differentiation.

    Evidence Luciferase reporters, promoter deletions, Sp3 ChIP, DNase-seq, and histone ChIP

    PMID:41456103

    Open questions at the time
    • ETS factor identity not pinned down
    • Link between expression decline and osteoclast function not established

Open questions

Synthesis pass · forward-looking unresolved questions
  • How coronin 1 functionally integrates its actin-binding, oligomerization, and phospho-switch states into the divergent calcineurin, IP3/Ca2+, cAMP/PKA, and PI3K outputs across cell types remains unresolved.
  • No unified biochemical model connecting oligomeric state to each signaling output
  • Direct enzymatic targets versus scaffolding roles not separated
  • Apparent opposite effects on cAMP (PDE4 versus Galphas) not mechanistically reconciled

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0008092 cytoskeletal protein binding 3 GO:0060090 molecular adaptor activity 3 GO:0098772 molecular function regulator activity 3 GO:0005198 structural molecule activity 2
Localization
GO:0005829 cytosol 3 GO:0005856 cytoskeleton 3 GO:0005886 plasma membrane 2 GO:0031410 cytoplasmic vesicle 2
Pathway
R-HSA-112316 Neuronal System 3 R-HSA-162582 Signal Transduction 3 R-HSA-168256 Immune System 3 R-HSA-5357801 Programmed Cell Death 2 R-HSA-5653656 Vesicle-mediated transport 2
Partners
ACTB (BETA-ACTIN)CDK5GNAS (GALPHAS)LPDC (MYCOBACTERIAL)PRKCA (PKCALPHA)TRIM67YWHAZ (14-3-3ZETA)
Complex memberships
coronin 1 homotrimer

Evidence

Reading pass · 31 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2007 Coronin 1 is actively recruited to mycobacterial phagosomes and blocks lysosomal delivery by activating the Ca2+-dependent phosphatase calcineurin; in coronin 1-deficient macrophages, calcineurin activity does not occur upon mycobacterial infection, resulting in lysosomal delivery and killing of mycobacteria. Coronin 1-deficient mouse macrophages, calcineurin activity assays, cyclosporin A/FK506 pharmacological inhibition, phagosome-lysosome fusion assays Cell High 17632055
2006 Coronin 1 exerts an inhibitory effect on cellular steady-state F-actin formation via an Arp2/3-dependent mechanism and is required for chemokine-mediated T cell migration, but is dispensable for T cell antigen receptor functions. Coronin 1-deficient (knockout) mice, F-actin quantification, chemotaxis assays, TCR functional assays Science High 16902139
2008 Upon T cell receptor triggering, coronin 1 is required for generation of inositol-1,4,5-trisphosphate (IP3) from PIP2, thereby enabling Ca2+ mobilization from intracellular stores, IL-2 production, T cell proliferation, and T cell survival. Coronin 1-deficient mice, IP3 measurement, Ca2+ mobilization assays, T cell functional assays (proliferation, IL-2 production, survival) Nature immunology High 18345003
2005 Coronin 1 forms coiled-coil-mediated homotrimeric complexes that associate with the plasma membrane via the N-terminal WD repeat domain and with the cytoskeleton via positively charged residues in a linker region between the WD domain and C-terminal coiled coil. Domain deletion mutagenesis, co-immunoprecipitation, fractionation assays, biochemical reconstitution of trimeric complexes Molecular biology of the cell High 15800061
2006 Crystal structure of murine coronin 1 (without leucine zipper) at 1.75 Å resolution reveals a seven-bladed beta propeller composed of five canonical WD40 repeats and two additional non-canonical blades; the C-terminal extension packs against the bottom of the propeller and is required for structural stability; conserved surface residues delineate potential F-actin binding sites. X-ray crystallography at 1.75 Å resolution Structure High 16407068
2005 Coronin 1 (p57) forms homodimers mediated by a leucine zipper structure in its C-terminal coiled-coil domain; mutation of two leucines in the zipper abolishes dimerization, and the C-terminal region enables co-localization with cortical F-actin only when full-length coronin 1 is co-expressed. Size-exclusion chromatography, sucrose density gradient, co-immunoprecipitation in COS-1 cells, leucine-to-alanine mutagenesis, GFP fusion co-localization The Biochemical journal High 15601263
2005 Coronin 1 is required for an early step of phagosome formation: TAT-WD dominant-negative constructs diminish lamellipodial extensions, actin accumulation at the base of bound particles, and Arp3 accumulation at the phagosome, without affecting opsonized RBC binding or receptor clustering. Dominant-negative TAT-fusion protein introduction into RAW 264.7 cells, phagocytosis assays, Arp3 localization by immunofluorescence Molecular biology of the cell Medium 15829569
2007 Mycobacterial lipoamide dehydrogenase C (LpdC) binds coronin 1 in a cholesterol-dependent manner (identified by GST-coronin 1 pulldown and mass spectrometry), retaining coronin 1 on the phagosomal membrane to arrest phagosome maturation; M. smegmatis overexpressing LpdC acquired the capacity to retain coronin 1 and prolong intracellular survival. GST pulldown, mass spectrometry identification, cell-free binding assays, IFNγ-induced phagolysosome fusion assays, M. smegmatis LpdC overexpression Journal of cell science Medium 17652161
2007 RNA interference knockdown of coronin 1 in J774 macrophages does not affect phagocytosis, macropinocytosis, cell locomotion, or NADPH oxidase activity, but causes rapid lysosomal delivery and killing of internalized mycobacteria, demonstrating that coronin 1's role in mycobacterial phagosome regulation is distinct from its (dispensable) role in actin-mediated processes. siRNA knockdown in J774 macrophages, phagocytosis/macropinocytosis/locomotion assays, NADPH oxidase activity assays, lysosomal delivery and bacterial killing assays Molecular biology of the cell High 18162581
2008 Phorbol ester (PMA) stimulation induces phosphorylation of p57/coronin 1 via protein kinase C, which reduces the protein's association with the actin cytoskeleton; phosphorylated coronin 1 showed lower affinity for F-actin in co-sedimentation assays, and during phagocytosis in differentiated HL60 cells coronin 1 dissociation from the actin cytoskeleton was concurrent with enhanced phosphorylation. Cell fractionation, 2D gel electrophoresis, co-sedimentation with F-actin, affinity chromatography, fluorescence microscopy in HL60 cells The Journal of biological chemistry High 18693254
2013 In sympathetic neurons, the NGF-TrkA signaling endosome induces expression and recruitment of Coronin 1 upon arrival at the cell body; Coronin 1 prevents premature lysosomal fusion of the signaling endosome (6–10-fold faster fusion in absence), enables endosomal recycling/re-internalization, and is required for NGF-TrkA-dependent calcium release, calcineurin activation, and CREB phosphorylation. Mouse sympathetic neuron culture with Coro1a KO, live imaging of endosome trafficking, lysosomal fusion kinetics, calcium imaging, calcineurin activity assays, CREB phosphorylation by Western blot Nature neuroscience High 24270184
2014 Coronin 1 interacts with the G protein subtype Gαs upon cell surface stimulation to activate the cAMP/PKA pathway in neurons; coronin 1-deficient mice lack cAMP/PKA-dependent presynaptic plasticity and exhibit neurobehavioral deficits that are rescued by membrane-permeable cAMP analogue infusion; coronin 1 mutants unable to bind Gαs fail to stimulate cAMP signaling. Co-immunoprecipitation (coronin 1–Gαs interaction), cAMP measurement, electrophysiology in amygdala, behavioral assays, in vivo cAMP analogue rescue in Coro1a KO mice, mutagenesis of Gαs-binding interface PLoS biology High 24667537
2011 Coronin 1 does not modulate F-actin levels in naive T cells and F-actin induction does not cause apoptosis; instead, coronin 1 provides pro-survival signals whose absence leads to rapid T cell apoptosis. Coronin 1 KO mice, F-actin quantification, apoptosis assays, pharmacological F-actin manipulation Journal of immunology Medium 21339362
2009 Coronin 1 is essential for IgM-induced intracellular Ca2+ mobilization and B cell proliferation upon BCR triggering, but is dispensable when costimulatory signals are present, consistent with normal in vivo B cell immune responses in coronin 1-deficient mice. Coronin 1 KO mice, Ca2+ mobilization assays, B cell proliferation assays, in vivo immunization Journal of immunology Medium 19201848
2012 Phosphorylation of coronin 1 at Thr-412 constitutively turns over in HL60 cells; the Thr-412-phosphorylated form fails to co-immunoprecipitate with β-actin, and the phosphomimetic T412D mutant shows reduced actin affinity compared to wild-type or the non-phosphorylatable T412A mutant, demonstrating that Thr-412 phosphorylation controls coronin 1–actin interaction. MALDI-TOF-MS, Phos-tag gel electrophoresis, site-directed mutagenesis (T412D, T412A), co-immunoprecipitation with β-actin, calyculin A treatment The Journal of biological chemistry High 23100250
2015 Upon cytokine stimulation of macrophages, phosphorylated coronin 1 transiently associates with 14-3-3ζ (and RACK1); 14-3-3ζ (but not RACK1) is required for coronin 1 relocation from the cell cortex to the cytoplasm, PI3-kinase activation, and induction of macropinocytosis. Co-immunoprecipitation of phospho-coronin 1 with 14-3-3ζ/RACK1, siRNA knockdown of 14-3-3ζ and RACK1, coronin 1 localization by imaging, PI3K activity assay, macropinocytosis assay The FEBS journal Medium 25645340
2014 Coronin 1 trimerization (via C-terminal coiled coil) is essential for protecting mycobacteria from lysosomal delivery and for calcineurin activation in macrophages; macrophage activation induces serine phosphorylation of coronin 1 that causes monomerization, thereby relieving protection. Trimerization-defective coronin 1 mutants expressed in macrophages, calcineurin activity assays, mycobacterial survival assays, phosphorylation analysis FEBS letters Medium 25217836
2016 Cyclin-dependent kinase 5 (CDK5) phosphorylates coronin 1 on Thr418/424, and this phosphorylation is required for coronin 1–Gαs association and subsequent cAMP/PKA pathway activation. Phosphorylation site mutagenesis (Thr418/424), CDK5 activity modulation, co-immunoprecipitation of coronin 1 with Gαs, cAMP measurement FEBS letters Medium 26823173
2015 In sympathetic neurons, Coronin 1 (downstream of NGF-TrkA) suppresses axon growth and branching by activating PLC-γ1-dependent calcium release, which releases PI3K-dependent suppression of GSK3β; Coro1a−/− mice display sympathetic axon overgrowth and overbranching in the developing heart. Coro1a KO mice, in vivo axon morphology analysis in developing heart, PLC-γ1/PI3K/GSK3β signaling pathway epistasis assays, calcium imaging The Journal of neuroscience Medium 25740518
2019 Coronin 1 deficiency increases cyclic AMP (cAMP) concentrations in T cells (by relieving PDE4-mediated cAMP degradation), which suppresses allo-specific T cell responses; costimulation induced on microbe-infected APCs overcomes cAMP-mediated immunosuppression to maintain anti-pathogen immunity. Coronin 1 KO mice, cAMP measurement, pharmacological cAMP/PDE4 modulation, allograft models, T cell transfer experiments Immunity High 30611611
2021 Coronin 1 maintains basal PI3Kδ activity in naive T cells, thereby suppressing caspase 8-mediated apoptosis; this pathway operates independently of TCR and IL-7 signaling. Coronin 1 KO mice, PI3Kδ activity assay, caspase 8 activity assay, genetic and pharmacological PI3Kδ inhibition, epistasis analysis with TCR/IL-7 pathways Science signaling Medium 34932374
2021 Coronin 1 (CORO1A) is phosphorylated at Thr-412 specifically by PKCα (not PKCβ) in human phagocytic cells; this phosphorylation prevents dissociation of coronin 1 from phagosomes when PKCα is inhibited, linking PKCα-mediated Thr-412 phosphorylation to coronin 1 intracellular redistribution during phagocytosis. PKC isoform-specific inhibitors, siRNA knockdown of PKCα vs. PKCβ, in vitro kinase assay with recombinant PKCα/PKCβ, confocal fluorescence microscopy of coronin 1 localization during phagocytosis Biochemistry and biophysics reports High 34189278
2015 A CORO1A frameshift mutation (S401fs) that replaces the last 61 C-terminal amino acids abolishes oligomerization and impairs cytoskeletal association of coronin 1 in patient lymphocytes, resulting in increased filamentous actin accumulation, severely defective thymic output, and impaired T cell survival, but normal calcium flux and cytotoxicity; demonstrating that C-terminal domain integrity is required for oligomerization and T cell homeostasis. Whole-genome sequencing, Western blot, biochemical oligomerization assay, cytoskeletal association assay (fractionation), flow cytometry of F-actin, T cell survival and thymic output analyses in patient cells The Journal of allergy and clinical immunology Medium 26476480
2004 Coronin 1 is in equilibrium between the cytosol and the cell cortex in T lymphocytes and accumulates in F-actin-rich membrane protrusions upon polarized TCR-CD3 stimulation, placing it at sites of actin remodeling during T cell activation. Subcellular fractionation, immunofluorescence and confocal microscopy of Coronin 1 localization in TCR-stimulated T cells International immunology Medium 14734608
2008 p57/coronin 1 and F-actin co-localize at sites of L. pneumophila adherence to macrophage-like cells (an actin polymerization-dependent event), but live L. pneumophila actively excludes p57/coronin 1 from its phagosome throughout infection, in contrast to opsonized zymosan or heat-killed bacteria which show transient coronin 1 accumulation. Fluorescence microscopy, cytochalasin D (actin polymerization inhibitor) treatment, comparison of live vs. heat-killed bacteria, U937 macrophage-like cells Biological & pharmaceutical bulletin Low 18451508
2023 CREBH transcriptionally represses Coro1a expression; overexpression of Coro1a in liver cells inhibited autophagic flux and elevated inflammatory cytokines upon palmitic acid stimulation, identifying Coro1a as a negative regulator of autophagosome-lysosome fusion in hepatocytes. Luciferase reporter assay (CREBH on Coro1a promoter), Coro1a overexpression in LO2 liver cells, autophagy flux assays (LC3-II, p62, LAMP1), inflammatory cytokine measurement Biochimica et biophysica acta. Molecular basis of disease Low 37837948
2025 Coronin 1A (Coro1A) localizes to growth cones and filopodia, interacts with TRIM67 (a brain-enriched E3 ubiquitin ligase that binds the netrin receptor DCC), and is required for netrin-1-dependent axon turning, branching, and corpus callosum development; a Coro1A mutant deficient in TRIM67 binding cannot rescue loss-of-Coro1A phenotypes. Coro1a KO mice, live imaging and immunofluorescence (growth cone localization), TRIM67-binding deficient Coro1A mutant rescue experiments, in vivo corpus callosum analysis The Journal of cell biology Medium 41085995
2020 In coronin 1-deficient platelets, integrin β2 translocation to the platelet surface upon thrombin stimulation is impaired, while morphological changes, Arp2/3 localization, and cAMP-dependent signaling are unaffected, indicating a specific role for coronin 1 in integrin β2 surface translocation. Coronin 1 KO mouse platelets, flow cytometry of integrin β2 surface expression, Arp2/3 localization by immunofluorescence, cAMP measurement International journal of molecular sciences Low 31948107
2025 Coro1a promotes MVB-plasma membrane fusion and exosome biogenesis by activating PKM2 to phosphorylate SNAP-23, which drives SNAP-23 to recruit STX-12 and VAMP7, increasing assembly of the STX-12-SNAP-23-VAMP7 SNARE complex; this function is independent of coronin 1 neddylation. Co-immunoprecipitation, siRNA knockdown of SNAP-23 and PKM2, PKM2 inhibitor, Coro1a KO in vivo tumor model, neddylation inhibitor MLN4924, EV quantification bioRxivpreprint Low
2009 Coronin 1 overexpression in differentiated PLB985 cells decreases mitochondrial depolarization and caspase-3/caspase-9 (but not caspase-8 or Bid cleavage) induced by gliotoxin or TRAIL, indicating that coronin 1 protects against the intrinsic (mitochondrial) apoptotic pathway. Coronin 1 overexpression in PLB985 cells, mitochondrial depolarization assay, caspase activity assays (caspase-3, -8, -9), Bid truncation Western blot Journal of immunology Medium 19454722
2026 Coro1a transcription requires both the core promoter (containing GC boxes bound by Sp3 transcription factor at GC box 4) and gene body/intronic regions (containing ETS-binding sequences in introns 1 and 2); mutations in intronic ETS sequences decrease Coro1a transcription, and active histone marks (H3K4me3, H3K27ac) at the intronic region decrease during macrophage-to-osteoclast differentiation concurrent with reduced Coro1a expression. Reporter (luciferase) assays, promoter deletion analysis, chromatin immunoprecipitation for Sp3, DNase sequencing (chromatin accessibility), histone modification ChIP, site-directed mutagenesis of ETS sites Journal of immunology Medium 41456103

Source papers

Stage 0 corpus · 70 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2007 Survival of mycobacteria in macrophages is mediated by coronin 1-dependent activation of calcineurin. Cell 240 17632055
2006 Requirement for coronin 1 in T lymphocyte trafficking and cellular homeostasis. Science (New York, N.Y.) 219 16902139
2008 Regulation of T cell survival through coronin-1-mediated generation of inositol-1,4,5-trisphosphate and calcium mobilization after T cell receptor triggering. Nature immunology 145 18345003
2003 Helicobacter pylori strains expressing the vacuolating cytotoxin interrupt phagosome maturation in macrophages by recruiting and retaining TACO (coronin 1) protein. Cellular microbiology 141 12542468
2005 Association of the leukocyte plasma membrane with the actin cytoskeleton through coiled coil-mediated trimeric coronin 1 molecules. Molecular biology of the cell 113 15800061
2006 The crystal structure of murine coronin-1: a regulator of actin cytoskeletal dynamics in lymphocytes. Structure (London, England : 1993) 101 16407068
2013 Coronin-1 is a neurotrophin endosomal effector that is required for developmental competition for survival. Nature neuroscience 79 24270184
2005 Coronin-1 function is required for phagosome formation. Molecular biology of the cell 78 15829569
2007 Lipoamide dehydrogenase mediates retention of coronin-1 on BCG vacuoles, leading to arrest in phagosome maturation. Journal of cell science 71 17652161
2014 Compound heterozygous CORO1A mutations in siblings with a mucocutaneous-immunodeficiency syndrome of epidermodysplasia verruciformis-HPV, molluscum contagiosum and granulomatous tuberculoid leprosy. Journal of clinical immunology 70 25073507
2009 Coronin-1 is associated with neutrophil survival and is cleaved during apoptosis: potential implication in neutrophils from cystic fibrosis patients. Journal of immunology (Baltimore, Md. : 1950) 64 19454722
2014 Coronin 1 regulates cognition and behavior through modulation of cAMP/protein kinase A signaling. PLoS biology 61 24667537
2004 Coronin-1 expression in T lymphocytes: insights into protein function during T cell development and activation. International immunology 56 14734608
2011 Migration and homeostasis of naive T cells depends on coronin 1-mediated prosurvival signals and not on coronin 1-dependent filamentous actin modulation. Journal of immunology (Baltimore, Md. : 1950) 49 21339362
2007 RNA interference in J774 macrophages reveals a role for coronin 1 in mycobacterial trafficking but not in actin-dependent processes. Molecular biology of the cell 45 18162581
2015 Recurrent viral infections associated with a homozygous CORO1A mutation that disrupts oligomerization and cytoskeletal association. The Journal of allergy and clinical immunology 42 26476480
2005 Homotypic dimerization of the actin-binding protein p57/coronin-1 mediated by a leucine zipper motif in the C-terminal region. The Biochemical journal 36 15601263
2009 Coronin 1 is essential for IgM-mediated Ca2+ mobilization in B cells but dispensable for the generation of immune responses in vivo. Journal of immunology (Baltimore, Md. : 1950) 33 19201848
2006 Localization of CORO1A in the macrophages containing Mycobacterium leprae. Acta histochemica et cytochemica 33 17327897
2009 Chemotaxis and phagocytosis in neutrophils is independent of coronin 1. Journal of immunology (Baltimore, Md. : 1950) 30 19234169
2019 Disruption of Coronin 1 Signaling in T Cells Promotes Allograft Tolerance while Maintaining Anti-Pathogen Immunity. Immunity 29 30611611
2008 Coronin 1 in innate immunity. Sub-cellular biochemistry 29 18925376
2009 Tryptophan aspartate-containing coat protein (CORO1A) suppresses Toll-like receptor signalling in Mycobacterium leprae infection. Clinical and experimental immunology 26 19438603
2018 Coronin 1 derived tryptophan-aspartic acid containing peptides inhibit membrane fusion. Chemistry and physics of lipids 25 30385272
2015 Coronin-1 and calcium signaling governs sympathetic final target innervation. The Journal of neuroscience : the official journal of the Society for Neuroscience 25 25740518
2011 Coronin 1-mediated naive T cell survival is essential for the development of autoimmune encephalomyelitis. Journal of immunology (Baltimore, Md. : 1950) 25 21289301
2015 Cytokine-induced macropinocytosis in macrophages is regulated by 14-3-3ζ through its interaction with serine-phosphorylated coronin 1. The FEBS journal 19 25645340
2014 Coronin 1 trimerization is essential to protect pathogenic mycobacteria within macrophages from lysosomal delivery. FEBS letters 15 25217836
2015 Regulation of immune cell homeostasis and function by coronin 1. International immunopharmacology 14 25882105
2013 Diverging role for coronin 1 in antiviral CD4+ and CD8+ T cell responses. Molecular immunology 14 23933558
2012 Antigen processing and presentation by dendritic cells is independent of coronin 1. Molecular immunology 12 23099476
2008 Phorbol ester-dependent phosphorylation regulates the association of p57/coronin-1 with the actin cytoskeleton. The Journal of biological chemistry 12 18693254
2024 TP53R175H mutation promotes breast cancer cell proliferation through CORO1A-P38 MAPK pathway regulation. Biochemical pharmacology 11 38331350
2020 "It Takes Two to Tango": Role of Neglected Macrophage Manipulators Coronin 1 and Protein Kinase G in Mycobacterial Pathogenesis. Frontiers in cellular and infection microbiology 11 33194820
2017 A Coronin 1-Dependent Decision Switch in Juvenile Mice Determines the Population of the Peripheral Naive T Cell Compartment. Journal of immunology (Baltimore, Md. : 1950) 11 28821585
2024 A Coronin 1-Derived Peptide Inhibits Membrane Fusion by Modulating Membrane Organization and Dynamics. The journal of physical chemistry. B 10 38739415
2022 CORO1A regulates lipoprotein uptake in Leydig cells exposed to cadmium. Ecotoxicology and environmental safety 10 35121256
2019 Coronin-1, King of Alloimmunity. Immunity 10 30650379
2014 VDR, RXR, Coronin-1 and Interferonγ Levels in PBMCs of Type-2 Diabetes Patients: Molecular Link between Diabetes and Tuberculosis. Indian journal of clinical biochemistry : IJCB 10 26089620
2012 Constitutive turnover of phosphorylation at Thr-412 of human p57/coronin-1 regulates the interaction with actin. The Journal of biological chemistry 10 23100250
2013 Coronin 1 is dispensable for leukocyte recruitment and liver injury in concanavalin A-induced hepatitis. Immunology letters 9 23856257
2024 Strategic Design of Tryptophan-Aspartic Acid-Containing Peptide Inhibitors Using Coronin 1 as a Template: Inhibition of Fusion by Enhancing Acyl Chain Order. The journal of physical chemistry. B 8 39268813
2021 Suppression of caspase 8 activity by a coronin 1-PI3Kδ pathway promotes T cell survival independently of TCR and IL-7 signaling. Science signaling 8 34932374
2024 TRIM4 enhances small-molecule-induced neddylated-degradation of CORO1A for triple negative breast cancer therapy. Theranostics 7 39629122
2020 Coronin 1 Is Required for Integrin β2 Translocation in Platelets. International journal of molecular sciences 7 31948107
2018 Getting in and Staying Alive: Role for Coronin 1 in the Survival of Pathogenic Mycobacteria and Naïve T Cells. Frontiers in immunology 7 30042765
2016 Activation of the cAMP/protein kinase A signalling pathway by coronin 1 is regulated by cyclin-dependent kinase 5 activity. FEBS letters 7 26823173
2021 Coronin-1 levels in patients with tuberculosis. The Indian journal of medical research 6 35662092
2024 Developing peptide-based fusion inhibitors as an antiviral strategy utilizing coronin 1 as a template. RSC medicinal chemistry 5 39399312
2023 CREBH promotes autophagy to ameliorate NASH by regulating Coro1a. Biochimica et biophysica acta. Molecular basis of disease 5 37837948
2023 Novel hemizygous CORO1A variant leads to combined immunodeficiency with defective platelet calcium signaling and cell mobility. The journal of allergy and clinical immunology. Global 5 37915722
2016 Role for coronin 1 in mouse NK cell function. Immunobiology 5 27717523
2008 Exclusion of actin-binding protein p57/coronin-1 from bacteria-containing phagosomes in macrophages infected with Legionella. Biological & pharmaceutical bulletin 5 18451508
2020 Induction of Allograft Tolerance While Maintaining Immunity Against Microbial Pathogens: Does Coronin 1 Hold a Key? Transplantation 4 31895336
2024 Kif15 regulates Coro1a+ cell migration and phagocytosis in zebrafish after spinal cord injury. International immunopharmacology 3 39709909
2022 Carbamazepine-modified HLA-A*24:02-bound peptidome: Implication of CORO1A in skin rash. International immunopharmacology 3 35526384
2021 Coronin-1 is phosphorylated at Thr-412 by protein kinase Cα in human phagocytic cells. Biochemistry and biophysics reports 3 34189278
2025 Coro1A and TRIM67 collaborate in netrin-dependent neuronal morphogenesis. The Journal of cell biology 2 41085995
2021 Major Outer Membrane Protein from Legionella pneumophila Inhibits Phagocytosis but Enhances Chemotaxis of RAW 264.7 Macrophages by Regulating the FOXO1/Coronin-1 Axis. Journal of immunology research 2 34812410
2025 Coronin 1 deficiency protects from the development of autoimmune myocarditis by reducing CD4+ T cells. ESC heart failure 1 40696745
2024 Coronin 1-dependent cell density sensing and regulation of the peripheral T cell population size. Oxford open immunology 1 38737939
2023 Coronin-1 promotes directional cell rearrangement in Drosophila wing epithelium. Cell structure and function 1 38030242
2026 Dual regulation of coronin-1 expression by the core promoter and intronic regions. Journal of immunology (Baltimore, Md. : 1950) 0 41456103
2026 Integrated analysis of programmed cell death-related genes identifies CORO1A as an apoptosis-associated gene in acute myeloid leukemia. PeerJ 0 42153148
2025 T cell population size control by coronin 1 uncovered: from a spot identified by two-dimensional gel electrophoresis to quantitative proteomics. Expert review of proteomics 0 39849824
2025 Coro1A and TRIM67 collaborate in netrin-dependent neuronal morphogenesis. bioRxiv : the preprint server for biology 0 40166342
2025 Role of CORO1A in Regulating Immune Homeostasis of Mammary Glands and Its Contribution to Clinical Mastitis Development in Dairy Cows. Biomolecules 0 40563467
2025 Coronin 1 Loss in Tumor Cells of Glioblastoma Patients Leads to Enhanced Tumor Aggressiveness. Journal of neurochemistry 0 41078258
2016 [Involvement of coronin-1 in foam cell formation of macrophages induced by mycolic acid and its mechanism]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 0 27053608
2010 [Construction of a siRNA plasmid for knockdown of coronin-1]. Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 0 20368107

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