| 2005 |
Targeted disruption of both Col8a1 and Col8a2 in mice demonstrates that type VIII collagen is required for normal anterior eye development: null mice show a thin corneal stroma with reduced fibroblastic cell layers, markedly thinned Descemet's membrane, enlarged and numerically reduced corneal endothelial cells, and decreased endothelial cell proliferation in response to growth factors in vitro, establishing a peri/subcellular matrix role for collagen VIII in supporting cell proliferation. |
Targeted gene knockout (Col8a1/Col8a2 double null mice), histology, in vitro proliferation assays |
FASEB journal |
High |
16051690
|
| 1991 |
The primary structure of the human COL8A1-encoded alpha1(VIII) chain was determined from genomic DNA sequencing; it shares the same domain structure (triple-helical and C-terminal non-triple-helical domains encoded within a single large exon) as the rabbit alpha1(VIII) chain, and the gene was mapped to the long arm of human chromosome 3. Type VIII collagen molecules are heterotrimers composed of alpha1(VIII) and alpha2(VIII) chains in a 2:1 ratio. |
Genomic DNA sequencing, chromosomal mapping (in situ hybridization) |
European journal of biochemistry |
High |
2029894
|
| 2024 |
COL8A1 secreted by THBS2+ cancer-associated fibroblasts directly interacts with the ITGB1 (integrin-β1) receptor on colorectal cancer cells, activating the PI3K/AKT signaling pathway, promoting EMT, and conferring oxaliplatin resistance; ITGB1 knockdown or AKT inhibition abrogates these effects. |
Single-cell RNA-seq, spatial transcriptomics, ITGB1 knockdown, AKT inhibitor treatment, in vitro/in vivo functional assays |
Molecular cancer |
Medium |
39732719
|
| 2022 |
In pancreatic ductal adenocarcinoma cells, COL8A1 activates ITGB1 and DDR1 receptors and downstream PI3K/AKT and NF-κB signaling; cJun/AP-1 was identified as a transcriptional activator of COL8A1 expression by ChIP assay. siRNA/shRNA-mediated COL8A1 inhibition reduced migration, invasion, and gemcitabine resistance, and reduced cytidine deaminase and thymidine kinase 2 expression; COL8A1-secreting CAFs rescued these phenotypes in vitro and in orthotopic xenograft models. |
siRNA/shRNA knockdown, ChIP assay, adhesion assays, EMSA binding studies, orthotopic xenograft transplantation |
Matrix biology |
Medium |
36375776
|
| 2022 |
COL8A1 promotes triple-negative breast cancer (TNBC) growth via FAK/Src activation; CRISPR-Cas9 knockout of COL8A1 inhibited spheroid/tumor growth and metastasis in vitro and in vivo, while exogenous recombinant COL8A1 protein promoted TNBC growth through FAK/Src signaling. COL8A1 expression is induced by hypoxia. |
CRISPR-Cas9 knockout, 3D spheroid culture, xenograft mouse models, immunoblotting, exogenous recombinant protein treatment |
Breast cancer research and treatment |
Medium |
35624176
|
| 2022 |
COL8A1 overexpression in MDA-MB-231 TNBC cells induces expression of IL1B and MMP1 through distinct signaling pathways; knockdown of either IL1B or MMP1 reduced invasion capacity of COL8A1-overexpressing cells, establishing that COL8A1 enhances invasion and metastasis via upregulation of IL1B and MMP1. |
DNA microarray, RT-qPCR, 3D invasion assay, siRNA knockdown, xenograft mouse models |
Biochemical and biophysical research communications |
Medium |
36577251
|
| 2021 |
Platelet-derived extracellular vesicles (pEVs) deliver miR-92a-3p to vascular smooth muscle cells, which promotes COL8A1 expression via the PTEN/PIP3/Akt pathway; Col8a1 knockdown in vivo abrogated the increase in carotid artery stiffness and simultaneously increased neointimal hyperplasia, demonstrating that Col8a1 is required for vascular stiffening after intimal injury. |
miRNA sequencing of pEVs, in vivo carotid artery injury model (rat), Col8a1 in vivo knockdown, Akt pathway inhibition, mRNA sequencing, IPA pathway analysis |
Frontiers in cell and developmental biology |
Medium |
33738288
|
| 2018 |
COL8A1 promotes proliferation of muscle-derived satellite cells (MDSCs) via the PI3K/AKT signaling pathway; CRISPR/Cas9-mediated activation of COL8A1 increased EdU incorporation and expression of cyclin B1 (CCNB1) and P-AKT, while repression reduced them. EGR1 was identified as a positive transcriptional regulator of COL8A1 by dual-luciferase reporter assay. |
CRISPR/Cas9 activation/repression, EdU labeling, Western blotting, dual-luciferase reporter assay |
Cell biology international |
Medium |
29696735
|
| 2018 |
COL8A1 protein localizes to Bruch's membrane in the mouse eye, as determined by immunohistochemistry. |
Immunohistochemistry on mouse eye sections |
Ophthalmology |
Low |
29706360
|
| 2009 |
siRNA-mediated knockdown of COL8A1 in the highly metastatic hepatocarcinoma cell line Hca-F significantly reduced cell proliferation, colony formation in soft agar, and invasion in vitro, and sensitized cells to D-limonene. |
siRNA knockdown, soft agar colony formation assay, invasion assay, drug sensitivity assay |
IUBMB life |
Low |
19109829
|
| 2022 |
COL8A1 promotes NSCLC proliferation and invasion through upregulation of IFIT1 and IFIT3, which mediate EGFR activation; COL8A1 knockdown reduced interferon response signaling, downregulated IFIT1/IFIT3, and suppressed EGFR activity in vitro and in vivo. |
COL8A1 knockdown/overexpression, in vitro cell growth/migration/apoptosis assays, in vivo tumor models, EGFR activity measurement |
Frontiers in oncology |
Low |
35280763
|
| 2024 |
COL8A1 knockdown in esophageal squamous carcinoma (ESCC) cells inactivated the PI3K/AKT pathway, reducing proliferation and invasion; COL8A1 overexpression restored PI3K/AKT activity and rescued proliferation and invasion, establishing PI3K/AKT as downstream of COL8A1 in ESCC. |
COL8A1 knockdown/overexpression, xenograft model, signaling pathway validation by western blot |
Annals of surgical oncology |
Low |
38429534
|
| 2024 |
COL8A1 overexpression in glioma cells promotes cell growth via activation of focal adhesion kinase (FAK) and downstream Akt and Erk1/2 phosphorylation; COL8A1 shRNA or CRISPR knockout reduced FAK/Akt/Erk1/2 phosphorylation, inhibited proliferation, and suppressed intracranial xenograft growth in mice. |
COL8A1 shRNA/CRISPR KO, immunoblotting, in vivo intracranial xenograft |
NPJ precision oncology |
Low |
39578589
|
| 2025 |
COL8A1 knockdown in human aortic endothelial cells (HAECs) suppressed endothelial gene programs, impaired tube formation, and enhanced NF-κB/Snail activation, promoting EndMT; conversely, recombinant COL8A1 protein or lentiviral overexpression preserved endothelial markers and attenuated TNF-α-induced EndMT. TNF-α had a biphasic effect on COL8A1 expression in HAECs (initial downregulation followed by upregulation), and COL8A1 was elevated in murine atherosclerotic lesions coinciding with decreased PECAM-1. |
siRNA knockdown, lentiviral overexpression, recombinant protein treatment, immunofluorescence, tube formation assay, single-cell RNA-seq |
American journal of physiology. Heart and circulatory physiology |
Medium |
41042748
|
| 2026 |
Secreted COL8A1 from COL8A1-positive cancer-associated fibroblasts engages integrin-β1 (ITGB1) on colorectal cancer tumor cells; silencing ITGB1 or COL8A1 abrogated EMT induction, reduced proliferation, and restored 5-FU sensitivity in vitro and in vivo, establishing a COL8A1/ITGB1-mediated EMT axis driving 5-FU resistance. |
CRISPR/Cas9, siRNA perturbations, conditioned media experiments, xenograft models, proliferation/migration/apoptosis assays |
Apoptosis |
Medium |
41784732
|
| 2025 |
In ADAMTSL4-deficient RPE cells, COL8A1/col8a1b is consistently upregulated, and COL8A1 knockdown in ADAMTSL4-deficient RPEs partially reverses the aberrant migratory phenotype, suggesting that COL8A1 acts downstream of ADAMTSL4 to promote cell migration. |
CRISPR/Cas9 adamtsl4 knockout zebrafish, siRNA knockdown of COL8A1, cell migration assay, transcriptomic profiling, fluorescence in situ hybridization |
bioRxivpreprint |
Low |
bio_10.1101_2025.11.03.686242
|
| 2025 |
FOSL2, an AP-1 family transcription factor, directly binds a conserved high-affinity motif in the COL8A1 promoter and transcriptionally activates COL8A1 expression in colorectal cancer; FOSL2 silencing suppresses COL8A1 expression, and FOSL2 and COL8A1 co-express at mRNA and protein levels in CRC specimens. |
Integrative transcriptomic analysis, promoter binding assay, siRNA knockdown of FOSL2, RT-qPCR, immunohistochemistry |
Biochemical and biophysical research communications |
Low |
41086679
|