Affinage

CKB

Creatine kinase B-type · UniProt P12277

Length
381 aa
Mass
42.6 kDa
Annotated
2026-06-09
80 papers in source corpus 19 papers cited in narrative 19 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CKB (brain-type creatine kinase) catalyzes the reversible transfer of phosphate between phosphocreatine and ADP to buffer cellular ATP, and the resulting locally regenerated ATP couples its enzymatic output to diverse energy-demanding processes (PMID:10854850, PMID:19333390). Within the catalytic core, His65 stabilizes the protein while a flexible loop containing Pro66 confers isoenzyme-specific substrate discrimination, accounting for CKB's distinct kinetic recognition of creatine versus cyclocreatine phosphate (PMID:10854850). Compartmentalized CKB activity supports actomyosin dynamics and cell motility: CKB accumulates in cortical membrane ruffles and supplies ATP that sustains F-actin polymerization and migration, a function repurposed by cancer cells where HIF-1-induced CKB drives invasion, invadopodia formation, and metastasis through its creatine kinase activity (PMID:19333390, PMID:32213653, PMID:35008190). In adipose tissue, CKB operates as a non-redundant effector of the futile creatine cycle, acting in parallel with UCP1 downstream of ADRA1A-Gαq and adrenergic signaling to mediate cold-triggered thermogenesis and whole-body energy expenditure (PMID:36344764, PMID:38272036). CKB additionally restrains PI3K-AKT signaling by binding AKT's PH domain through an 84-amino-acid C-terminal fragment and sequestering it from mTOR-dependent activation, which in turn suppresses mitochondrial calcium accumulation and mPTP opening to favor mitochondrial ATP production (PMID:34706306, PMID:38896801). CKB protein levels are set by multiple inputs: the ASB9 E3 ubiquitin ligase recognizes CKB via its ankyrin repeat domain and targets it for polyubiquitination and proteasomal degradation, while IGF2BP2 stabilizes CKB mRNA (PMID:22418839, PMID:38341962). CKB is also required for ciliary axoneme function via interaction with Ribc2 and for brain energy homeostasis underlying synaptic plasticity and cognition (PMID:36508087, PMID:39816485).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 2000 High

    Resolved how CKB achieves isoenzyme-specific substrate recognition, distinguishing residues governing catalysis from those governing stability.

    Evidence Site-directed mutagenesis of CKB in COS-7 cells with substrate kinetics and stability assays

    PMID:10854850

    Open questions at the time
    • No full-length crystal structure of CKB itself reported here
    • Physiological consequence of substrate discrimination not tested in cells
  2. 2009 Medium

    Established that CKB is not merely cytosolic but acts in a spatially compartmentalized manner at cortical membrane sites to fuel motility.

    Evidence Live-cell imaging and CKB-deficient fibroblast complementation with targeted relocalization constructs

    PMID:19333390

    Open questions at the time
    • Molecular anchor recruiting CKB to membrane ruffles not identified
    • Direct link to specific actomyosin components not defined
  3. 2012 Medium

    Defined the structural basis by which CKB protein stability is controlled, mapping the ASB9 substrate-receptor interface that routes CKB to degradation.

    Evidence X-ray crystallography of hASB9-2, docking, and truncation/mutagenesis binding assays

    PMID:22418839

    Open questions at the time
    • In-cell ubiquitination reconstitution not described
    • Conditions triggering CKB degradation in vivo unknown
  4. 2020 Medium

    Showed CKB-generated ATP is required for actin remodeling and migration in a hormone-responsive epithelial context, generalizing the cortical-energy model.

    Evidence CKB knockdown/overexpression with rescue in Ishikawa cells, ATP and F-actin assays under GnRH-antagonist treatment

    PMID:32213653

    Open questions at the time
    • Mechanistic link between GnRH signaling and CKB transcription not resolved
    • In vivo relevance not tested
  5. 2021 Medium

    Revealed a non-catalytic moonlighting function: CKB binds and sequesters AKT to restrain its activation, connecting CKB to growth signaling beyond ATP buffering.

    Evidence Co-IP, domain mapping, 84aa fragment overexpression, xenografts, and MD simulation in prostate cancer cells

    PMID:34706306

    Open questions at the time
    • Whether endogenous full-length CKB stoichiometrically controls AKT in vivo unclear
    • Relationship to CKB enzymatic activity not separated
  6. 2022 High

    Identified CKB as an essential downstream effector of adrenergic thermogenic signaling via the futile creatine cycle.

    Evidence Adipocyte-selective CKB knockout in vivo, energy-expenditure measurement, adrenergic pathway dissection

    PMID:36344764

    Open questions at the time
    • Molecular target of CKB-driven ATP turnover in the futile cycle not fully defined
    • Subcellular site of thermogenic CKB activity not pinpointed
  7. 2024 High

    Established genetic non-redundancy between CKB and UCP1, defining CKB as an independent arm of brown-fat thermogenesis.

    Evidence Inducible adipocyte-selective Ucp1/Ckb co-deletion and cold-tolerance phenotyping

    PMID:38272036

    Open questions at the time
    • Quantitative contribution of CKB versus UCP1 across temperatures unresolved
    • Tissue-specific creatine flux not directly measured
  8. 2024 Medium

    Connected CKB's AKT inhibition to mitochondrial homeostasis, showing it suppresses mitochondrial calcium and mPTP opening to sustain ATP production.

    Evidence CKB siRNA, mCa2+ and mPTP assays, AKT activity, F1F0 inhibitor sensitivity, isoform comparison vs CKMT1A/B, mouse tumor model

    PMID:38896801

    Open questions at the time
    • Direct biochemical chain from AKT to mCa2+ channels not fully defined
    • Isoform-specificity mechanism not explained
  9. 2024 Medium

    Demonstrated CKB is required for structural synaptic plasticity and cognition, linking creatine-kinase activity to brain energy homeostasis.

    Evidence AAV-mediated CKB knockdown in mouse brain with behavioral, oxidative-stress, and spine-morphology readouts

    PMID:39816485

    Open questions at the time
    • Cell-type-specific contribution within brain not dissected
    • Partial (34%) knockdown limits quantitative interpretation
  10. 2024 Medium

    Identified a post-transcriptional mechanism setting CKB abundance via IGF2BP2-mediated mRNA stabilization.

    Evidence RIP-seq and actinomycin D decay assays with IGF2BP2 manipulation in ccRCC, in vitro and in vivo

    PMID:38341962

    Open questions at the time
    • Whether mRNA stabilization is the dominant route of CKB induction in other tissues unknown
    • Binding site on CKB transcript not mapped
  11. 2022 Medium

    Identified the ciliary axoneme as a distinct CKB compartment, requiring Ribc2 for localization and supporting ciliary motility.

    Evidence IP/MS, morpholino knockdown, and fluid-flow assays in Xenopus laevis ortholog

    PMID:36508087

    Open questions at the time
    • Human CKB ciliary role not directly confirmed
    • Mechanism by which Ribc2 anchors CKB unknown
  12. 2025 Medium

    Showed CKB protein stability can be pharmacologically enhanced to boost thermogenesis, validating CKB as a druggable thermogenic node.

    Evidence Photoaffinity labeling, click chemistry, and SPR with celastrol in human brown adipocytes

    PMID:40213647

    Open questions at the time
    • Binding site on CKB not mapped
    • Mechanism of stabilization at structural level undefined

Open questions

Synthesis pass · forward-looking unresolved questions
  • How CKB's enzymatic ATP-buffering function and its non-catalytic AKT-sequestering function are coordinated and partitioned across subcellular compartments remains unresolved.
  • No structure separating catalytic and AKT-binding functions in full-length CKB
  • Tissue-specific balance between these roles unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 2 GO:0140096 catalytic activity, acting on a protein 2 GO:0098772 molecular function regulator activity 1
Localization
GO:0005829 cytosol 2 GO:0005886 plasma membrane 1 GO:0005929 cilium 1
Pathway
R-HSA-1430728 Metabolism 2 R-HSA-162582 Signal Transduction 2
Partners
AKT1ASB9IGF2BP2PRDX2RIBC2

Evidence

Reading pass · 19 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2009 CK-B (CKB) transiently accumulates in membrane ruffles of astrocytes and fibroblasts during spreading and migration, and ablation of CKB activity impairs spreading and migration performance. Complementation experiments using forced relocalization of CKB from cytosol to cortical membrane sites confirmed that compartmentalized CKB-generated ATP locally supports actomyosin dynamics and cell motility. Live-cell imaging, CKB-deficient fibroblast complementation with targeted protein relocalization constructs, migration/spreading assays PloS one Medium 19333390
2021 CKB interacts directly with AKT and sequesters it from activation by mTOR. The interaction is mediated by an 84-amino acid C-terminal fragment of CKB binding to AKT's PH domain. Ectopic expression of this 84aa fragment inhibits AKT activation, EMT, and cell proliferation in prostate cancer cells. Molecular dynamics simulation on crystal structures independently confirmed this interaction interface. Co-immunoprecipitation, kinase cDNA screen, domain mapping with truncation mutants, ectopic 84aa fragment overexpression, xenograft tumor models, molecular dynamics simulation Neoplasia (New York, N.Y.) Medium 34706306
2022 ADRA1A-Gαq signaling (triggered by noradrenaline) induces expression of thermogenic genes of the futile creatine cycle including CKB, and CKB is required as an effector protein for this thermogenic output. Combined Gαq and Gαs signaling in adipocytes promotes whole-body energy expenditure in a CKB-dependent manner. Adipocyte-selective genetic knockout of CKB in vivo, whole-body energy expenditure measurement, gene expression analysis, adrenergic receptor signaling pathway dissection Nature metabolism High 36344764
2024 CKB functions in parallel with UCP1 as a non-redundant mediator of cold-triggered adipocyte thermogenesis. Inducible adipocyte-selective co-deletion of both Ucp1 and Ckb exacerbates cold intolerance beyond either single deletion, establishing CKB as an independent effector of UCP1-independent thermogenesis in brown adipocytes. Inducible adipocyte-selective Ucp1 knockout, inducible co-deletion of Ucp1 and Ckb, cold-tolerance challenge, body temperature measurement Cell metabolism High 38272036
2024 CKB promotes mitochondrial ATP production by suppressing mitochondrial calcium (mCa2+) levels, thereby preventing mitochondrial permeability transition pore (mPTP) activation. CKB achieves mCa2+ suppression through inhibition of AKT activity. Silencing CKB (but not CKMT1A or CKMT1B) causes loss of sensitivity to F1F0 ATP synthase inhibition, linking CKB specifically to this pathway. CKB siRNA knockdown, mitochondrial calcium measurement, mPTP activity assay, AKT activity measurement, F1F0 ATP synthase inhibitor sensitivity assay, in vivo mouse tumor model Advanced science (Weinheim, Baden-Wurttemberg, Germany) Medium 38896801
2012 Crystal structure of hASB9-2 (an isoform of the CKB-targeting E3 ubiquitin ligase substrate receptor ASB9) was solved at 2.2-Å resolution. Amino acid substitution analysis based on docking showed His103 and Phe107 in hASB9-2 are essential for binding to CKB. Truncation analysis showed the first six ankyrin repeats plus the N-terminal region of hASB9-2 are required for CKB interaction. ASB9 recognizes CKB via its ankyrin repeat domain and mediates CKB polyubiquitination and proteasomal degradation via its SOCS box domain. X-ray crystallography (2.2 Å), site-directed mutagenesis, docking, truncation mutant binding assays The protein journal Medium 22418839
2000 His65 in human CK-BB (CKB) contributes to protein stability rather than catalysis: the ΔH65 mutant retains near-normal substrate affinity but shows very low stability. The double mutant ΔH65P66 shows eight-fold decreased affinity for creatine phosphate and inability to dephosphorylate cyclocreatine phosphate, indicating that the flexible loop containing Pro66 accounts for isoenzyme-specific substrate discrimination. Site-directed mutagenesis of CKB expressed in COS-7 cells, substrate affinity (Km) measurements, cyclocreatine phosphate dephosphorylation assay, stability assay FEBS letters High 10854850
2020 GnRH antagonist (GnRH-ant) reduces CKB expression in endometrial epithelial cells, leading to decreased ATP generation, F-actin depolymerization, and impaired cell migration. CKB knockdown phenocopies these effects; CKB overexpression rescues both the GnRH-ant-induced and knockdown-induced defects in actin polymerization and migration. CKB knockdown and overexpression in Ishikawa cells, ATP measurement, F-actin/G-actin assay, cell migration assay, in vitro GnRH-ant treatment Reproduction (Cambridge, England) Medium 32213653
2021 CKB expression is induced downstream of HIF-1 in mammary tumor cells. CKB is necessary for breast cancer cell invasion in vitro and promotes tumor growth and lung metastasis in vivo. Cyclocreatine (a creatine kinase activity inhibitor) represses cell migration, invasion, invadopodia formation, and lung metastasis, indicating that CKB creatine kinase enzymatic activity mediates these pro-metastatic effects. HIF-1 WT vs knockout mammary tumor cell gene screen, CKB loss/gain-of-function, invasion assays, invadopodia assay, in vivo lung metastasis model, cyclocreatine pharmacological inhibition Cancers Medium 35008190
2024 Creatine supplementation increases CKB-BB activity and expression in brain, and AAV-mediated CKB knockdown (34% reduction) in mouse brain causes deficits in learning/memory, oxidative stress, and hippocampal spine morphology damage, establishing CKB as a required mediator of structural synaptic plasticity and cognitive function in the brain. AAV-directed CKB knockdown in mouse brain, behavioral cognitive testing, hippocampal spine morphology analysis, CKB activity measurement, creatine dietary supplementation Food science & nutrition Medium 39816485
2022 Ckb (CKB ortholog in Xenopus laevis) localizes to the axoneme of multi-cilia on embryonic epithelium and interacts with Ribc2 (identified by IP/MS). Morpholino-mediated knockdown of Ckb results in abnormal ciliary beating and reduced cilia-driven fluid flow; Ckb localization at the ciliary axoneme is dependent on Ribc2. Immunoprecipitation/mass spectrometry (IP/MS), antisense morpholino knockdown in Xenopus, fluorescent bead fluid-flow assay, immunostaining, western blot of flag-tagged proteins Genes & genomics Medium 36508087
2004 In mouse brain, CK-B (CKB) protein is selectively expressed in astrocytes among glial populations and in inhibitory neurons among neuronal populations, as determined by immunohistochemistry. This distribution is complementary to GAMT (expressed in oligodendrocytes/astrocytes) and uCK-Mi (expressed in neurons), suggesting CKB in astrocytes buffers energy for cells highly resistant to hypoxia/hypoglycemia. Immunohistochemistry in mouse brain tissue sections The European journal of neuroscience Low 15245487
2016 Increased CKB promoter methylation is associated with decreased CKB mRNA and protein expression in gastric cancer. In hematologic malignancies, CKB mRNA expression correlates with CKB promoter unmethylated status, indicating DNA methylation as a regulatory mechanism for CKB expression. Bisulfite sequencing/methylation analysis, RT-PCR for CKB mRNA, western blot for protein, correlation with promoter methylation status PloS one Low 26460485
2005 CKB mRNA expression in peripheral blood leukemia blasts correlates with serum CK-BB activity, and CKB promoter hypomethylation is associated with higher CKB mRNA expression in cancer cell lines, supporting promoter DNA methylation as a mechanism regulating CKB transcription. RT-PCR, CK isoenzyme analysis, bisulfite methylation analysis of CKB promoter, correlation analysis Clinica chimica acta; international journal of clinical chemistry Low 15996648
2025 Celastrol directly binds CKB protein (confirmed by photoaffinity labeling, click chemistry, and surface plasmon resonance), increasing CKB protein stability and modulating the futile creatine cycle in human brown adipocytes to promote thermogenesis. Photoaffinity labeling (PAL), click chemistry, surface plasmon resonance (SPR), celastrol-based small molecule probe Metabolism open Medium 40213647
2016 CKB (CKBB) interacts with Peroxiredoxin II (Prx II) under physiological and heat-stress conditions in A549 and HeLa cells, as demonstrated by co-immunoprecipitation. Heat treatment enhances this association, and Prx II oligomerization under thermal stress may protect CKB enzyme activity from heat-induced inactivation. Co-immunoprecipitation (Co-IP) of overexpressed HA-Prx II and Flag-CK BB in A549 and HeLa cell lysates, heat stress treatment Ukrainian biochemical journal Low 29227081
2025 During RANKL-induced osteoclastogenesis, CKB expression is upregulated. ACAT1 inhibitor Avasimibe downregulates CKB expression and consequently inhibits the PI3K-AKT signaling pathway, suppressing osteoclast differentiation. Exogenous phosphocreatine reverses the inhibitory effects of Avasimibe on osteoclast differentiation and PI3K-AKT activation, placing CKB upstream of PI3K-AKT in the osteoclastogenesis pathway. RNA-seq, western blot, TRAP staining, phosphocreatine rescue experiment, OVX mouse model, CKB knockdown International immunopharmacology Medium 41352098
2024 IGF2BP2 (an RNA-binding protein) enhances CKB mRNA stability by directly binding to CKB mRNA (demonstrated by RIP-seq), thereby increasing CKB expression. This mechanism underlies IGF2BP2-mediated suppression of ccRCC metastasis. RIP-seq, actinomycin D mRNA stability assay, IGF2BP2 knockdown/overexpression, migration/invasion assays in vitro and in vivo Translational oncology Medium 38341962
2022 CKB overexpression suppresses IL-1β-induced chondrocyte senescence in vitro by upregulating RAP1 expression, thereby activating the PI3K/AKT signaling pathway; this anti-senescence effect is reversed by pathway inhibition (rescue experiments). CKB overexpression also delays OA progression in a rat knee joint model in vivo. Lentiviral CKB overexpression, IL-1β senescence model, RAP1/PI3K/AKT pathway analysis by western blot, rescue experiments, rat OA in vivo model Cellular signalling Low 42128154

Source papers

Stage 0 corpus · 80 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2004 Distinct cellular expressions of creatine synthetic enzyme GAMT and creatine kinases uCK-Mi and CK-B suggest a novel neuron-glial relationship for brain energy homeostasis. The European journal of neuroscience 135 15245487
1981 Several conditions causing elevation of serum CK-MB and CK-BB. American journal of clinical pathology 75 7234757
2024 Parallel control of cold-triggered adipocyte thermogenesis by UCP1 and CKB. Cell metabolism 69 38272036
2022 ADRA1A-Gαq signalling potentiates adipocyte thermogenesis through CKB and TNAP. Nature metabolism 52 36344764
2009 Local ATP generation by brain-type creatine kinase (CK-B) facilitates cell motility. PloS one 48 19333390
1994 Neural tissue-related proteins (NSE, G0 alpha, 28-kDa calbindin-D, S100b and CK-BB) in serum and cerebrospinal fluid after cardiac arrest. Journal of the neurological sciences 45 8064306
1982 Assessment of neurologic outcome in asphyxiated term infants by use of serial CK-BB isoenzyme measurement. The Journal of pediatrics 43 7143180
1979 Detection of myocardial infarct extension by CK-B radioimmunoassay. Circulation 34 758995
1991 Localization and genetic linkage of the human immunoglobulin heavy chain genes and the creatine kinase brain (CKB) gene: identification of a hot spot for recombination. Genomics 33 1674725
2015 Deregulated Expression of SRC, LYN and CKB Kinases by DNA Methylation and Its Potential Role in Gastric Cancer Invasiveness and Metastasis. PloS one 32 26460485
2021 Virus-CKB: an integrated bioinformatics platform and analysis resource for COVID-19 research. Briefings in bioinformatics 28 32715315
2021 CKB inhibits epithelial-mesenchymal transition and prostate cancer progression by sequestering and inhibiting AKT activation. Neoplasia (New York, N.Y.) 28 34706306
1982 Serum CK-BB isoenzyme in preterm infants with periventricular hemorrhage. The Journal of pediatrics 26 7062183
1979 The significance of creatine kinase (CKBB) in metastatic cancer of the prostate. Annals of clinical and laboratory science 25 485095
2012 Energy dysfunction in Huntington's disease: insights from PGC-1α, AMPK, and CKB. Cellular and molecular life sciences : CMLS 24 22627493
1985 Elevated CK-MB and CK-BB in serum and tumor homogenate of a patient with lung cancer. Clinical cardiology 21 2985311
1980 Circulating CK-MB and CK-BB isoenzymes after prostate resection. Clinical chemistry 20 7356573
1981 Studies on the change of electrophoretic mobility and the decay of catalytic activity of brain-type creatine kinase isoenzyme (CK-BB) following incubation at 37 degrees C. Clinica chimica acta; international journal of clinical chemistry 19 7261406
2020 GnRH antagonist alters the migration of endometrial epithelial cells by reducing CKB. Reproduction (Cambridge, England) 17 32213653
1979 Increased CK-BB concentrations in serum following cardiac surgery. Clinica chimica acta; international journal of clinical chemistry 17 436298
1994 Kinetics of creatine uptake in the perfused mouse liver: a 31P-n.m.r. study of transgenic mice expressing creatine kinase (CKBB) in the liver. The Biochemical journal 15 7980414
2016 The neonatal levels of TSB, NSE and CK-BB in autism spectrum disorder from Southern China. Translational neuroscience 14 28123815
1991 Insulin responsiveness of CK-M and CK-B mRNA in the diabetic rat heart. The American journal of physiology 14 1887884
2024 CKB Promotes Mitochondrial ATP Production by Suppressing Permeability Transition Pore. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 13 38896801
2021 HIF-Dependent CKB Expression Promotes Breast Cancer Metastasis, Whereas Cyclocreatine Therapy Impairs Cellular Invasion and Improves Chemotherapy Efficacy. Cancers 13 35008190
2020 Bioelectrochemical chlorate reduction by Dechloromonas agitata CKB. Bioresource technology 13 32688253
1982 Creatine kinase isoenzyme (CK-BB) in combination to prostatic acid phosphatase measured by RIA in the diagnosis of prostatic cancer. Urological research 13 6180539
1999 Comparison between creatine kinase brain isoenzyme (CKBB) activity and Sarnat score for prediction of adverse outcome following perinatal asphyxia. Journal of perinatal medicine 12 10732307
1991 Evaluation of carcinoembryonic antigen (CEA) and brain-type creatine kinase (CK-BB) in serum from patients with carcinoma of the lung. Neoplasma 12 1645850
1982 Circulating CK-MB and CK-BB isoenzyme after gastrointestinal surgery. Journal of clinical pathology 12 7068911
2012 Crystal structure of Human ASB9-2 and substrate-recognition of CKB. The protein journal 11 22418839
2005 Increased creatine kinase BB activity and CKB mRNA expression in patients with hematologic disorders: relation to methylation status of the CKB promoter. Clinica chimica acta; international journal of clinical chemistry 10 15996648
1987 A novel CK isoenzyme migrating between CK-MB and CK-BB. Clinical biochemistry 10 3552321
1986 Comparisons between CK-B and other clinical indicators of cardiac contusion following multiple trauma. Scandinavian journal of thoracic and cardiovascular surgery 10 3704604
1992 Characterization of a new hybrid mink-mouse clone panel: chromosomal and regional assignments of the GLO, ACY, NP, CKBB, ADH2, and ME1 loci in mink (Mustela vison). Mammalian genome : official journal of the International Mammalian Genome Society 9 1352156
1988 A sensitive bioluminescent immunoinhibition test for CK-B subunit activity and a CK-MB specific ELISA compared: correlation with agarose electrophoresis and influence of CK-isoenzyme profile on results. Clinical chemistry 9 3390922
2024 RNA-binding protein IGF2BP2 suppresses metastasis of clear cell renal cell carcinoma by enhancing CKB mRNA stability and expression. Translational oncology 8 38341962
2000 Creatine kinase isoenzymes specificities: histidine 65 in human CK-BB, a role in protein stability, not in catalysis. FEBS letters 8 10854850
2022 CKB affects human osteosarcoma progression by regulating the p53 pathway. American journal of cancer research 7 36381321
2015 Cyanobacterial KnowledgeBase (CKB), a Compendium of Cyanobacterial Genomes and Proteomes. PloS one 7 26305368
1997 Assignment of TCF1, TGM1, CALM1, CKB, THBS1, B2M, and FES in Ateles paniscus chamek (Platyrrhini, Primates). Cytogenetics and cell genetics 7 9533020
1988 Immunoprecipitation method for CK-MB analysis re-evaluated: influence of CK-BB and macro-CK on blank activities. Clinical chemistry 7 3280165
1988 Total creatine kinase (CK) and CK-B activity in maternal blood and cord-blood samples after vaginal and cesarean births. Clinical chemistry 6 3390927
1989 Skeletal muscle CK-B activity in neurogenic muscular atrophies. Journal of neurology 5 2760646
1988 Regional mapping of the creatine kinase b (CKBB) gene in rabbit (Oryctolagus cuniculus) and man using a rat cDNA probe. Cytogenetics and cell genetics 5 3234040
1988 Creatine kinase (CK-BB) determination in cerebrospinal fluid after acute experimental head injury. British journal of neurosurgery 5 3267300
1987 EcoRI RFLP at the creatine kinase-brain type gene locus (CKBB, chromosome 14). Nucleic acids research 5 2885812
1986 Serial measurement of arterial and internal jugular venous creatine kinase isoenzyme BB (CK-BB) after deep hypothermic total circulatory arrest in pediatric cardiac surgery. The Thoracic and cardiovascular surgeon 5 2429388
2025 Long-Term Creatine Supplementation Improves Cognitive and Hippocampal Structural Plasticity Impairments in a D-Gal-Induced Aging Model via Increasing CK-BB Activity in the Brain. Food science & nutrition 4 39816485
2020 Pain-CKB, A Pain-Domain-Specific Chemogenomics Knowledgebase for Target Identification and Systems Pharmacology Research. Journal of chemical information and modeling 4 32786694
1995 Elevated serum CK-MB and CK-BB isoenzymes in a patient with small cell lung cancer: a case report. Zhonghua yi xue za zhi = Chinese medical journal; Free China ed 4 7780887
1986 Serum creatine-kinase-BB and brain damage in high-risk newborn infants. Preliminary study with a new method for CK-BB estimation. American journal of perinatology 4 3718634
2025 Celastrol targets CKB-mediated futile creatine cycle in human brown adipocytes thermogenesis. Metabolism open 3 40213647
2020 Pain Chemogenomics Knowledgebase (Pain-CKB) for Systems Pharmacology Target Mapping and Physiologically Based Pharmacokinetic Modeling Investigation of Opioid Drug-Drug Interactions. ACS chemical neuroscience 3 32966035
1993 Cranial ultrasonography, CK-BB and neurological assessment as predictors of outcome in hypoxic ischaemic encephalopathy. Annals of tropical paediatrics 3 7687117
1991 [S100a0 protein and creatine kinase isozymes (CK-B & CK-M) in serum and urine might be useful markers for non specific tissue damages by extracorporeal shockwave lithotripsy (ESWL)]. Nihon Hinyokika Gakkai zasshi. The japanese journal of urology 3 1921018
1990 Serum CK-BB activity in the preterm infant and outcome at two and four years of age. Developmental medicine and child neurology 3 2365144
1989 Biochemical markers in the acute stage of head injury--aldosterone and CK-BB. Neurologia medico-chirurgica 3 2477723
1989 Aldosterone, catecholamines, and CK-BB as biochemical markers in hypertensive intracerebral hemorrhage. Neurologia medico-chirurgica 3 2483868
1985 Creatine kinase isoenzyme (CK-BB) by RIA as a follow-up marker in stage D cancer of the prostate: comparison with PAP RIA. Journal of surgical oncology 3 3974250
1984 Creatine kinase isoenzymes in serum from children--especially focusing on CK-BB. Scandinavian journal of clinical and laboratory investigation 3 6484482
2022 Virus-CKB 2.0: Viral-Associated Disease-Specific Chemogenomics Knowledgebase. ACS omega 2 36312370
2022 Ckb and Ybx2 interact with Ribc2 and are necessary for the ciliary beating of multi-cilia. Genes & genomics 2 36508087
1994 CK-BB as indicator of prenatal brain-cell injury in fetuses with absent or reverse end-diastolic flow velocities of the umbilical arteries. Journal of perinatal medicine 2 7823262
1987 CKBB-enzyme activity in serum in neonates born after vaginal delivery and cesarean section. Neuropediatrics 1 3683752
1983 [Activity of the creatine kinase isoenzyme CK-BB in the serum of neonates as an indicator of perinatal damage to the central nervous system]. Monatsschrift Kinderheilkunde : Organ der Deutschen Gesellschaft fur Kinderheilkunde 1 6348519
2026 METTL3-mediated m6A modification of circWDR85 drives breast cancer bone metastasis via the CKB/c-Jun axis. Bone 0 41912059
2026 CKB overexpression mitigates osteoarthritis by delaying chondrocyte senescence via activation of the RAP1/PI3K/AKT signaling pathway. Cellular signalling 0 42128154
2025 Myocardial damage in a 4-year old who ingested bisoprolol and hydrochlorothiazide - Incidental CK-BB highlighted other tissue toxicity. Clinical biochemistry 0 41076179
2025 Platelet CKB as a potential contributor to serum creatine kinase abnormalities and metastasis in cancer patients. Clinical and experimental medicine 0 41217562
2025 ACAT1 inhibitor Avasimibe suppresses osteoclastogenesis and alleviates ovariectomy-induced bone loss via CKB/PI3K-AKT signaling. International immunopharmacology 0 41352098
2024 [Effects of plumbagin on proliferation and apoptosis of human hepatocellular carcinoma cells based on CKB/p53 signaling pathway]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 0 38812135
2016 Prx II and CKBB proteins interaction under physiologic al and thermal stress conditions in A549 and HeLa cells. Ukrainian biochemical journal 0 29227081
1997 [Interference of CK-BB isoenzyme in the determination of CK-MB using the immunoinhibition method in patients with pulmonary diseases]. Lijecnicki vjesnik 0 9531757
1992 Total CK and CK-BB activity in serum from sheep with scrapie. The veterinary quarterly 0 1485407
1991 NcoI RFLP in human brain creatine kinase gene (CKBB). Nucleic acids research 0 1672741
1991 [Isolation and purification of human brain creatine kinase (CK-BB)]. Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae 0 1839527
1989 [The determination of creatine kinase isoenzyme subunits CK-M and CK-B with the help of precipitating antibodies]. Zeitschrift fur medizinische Laboratoriumsdiagnostik 0 2711729
1984 [Creatine kinase and creatine kinase isoenzymes in the serum of newborn infants. 1. Effect of the mode of delivery on total CK and CK-B]. Zentralblatt fur Gynakologie 0 6711179
1980 [Measurement of CK-BB isoenzyme after cardiac surgery (author's transl)]. Anesthesie, analgesie, reanimation 0 7469066

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