| 2002 |
CHODL encodes a type I transmembrane protein with a single C-type lectin carbohydrate recognition domain (CRD) in its extracellular portion; it is N-glycosylated (~36 kDa) and shows predominantly perinuclear localization in transiently transfected COS1 cells; no specific interaction with hyaluronan was detected. |
PCR cloning, Northern blot, RT-PCR, immunohistochemistry, Western blot, transient transfection with subcellular localization imaging, cetylpyridinium chloride precipitation (negative for hyaluronan binding) |
Genomics |
Medium |
12079284
|
| 2003 |
An alternatively spliced CHODL isoform lacking the transmembrane domain (CHODLfΔE/CHODLΔe) is expressed exclusively in T lymphocytes and is regulated during T lymphopoiesis; the transmembrane-containing isoform CHODLf colocalizes with rBet1 to the endoplasmic reticulum–Golgi apparatus. |
RT-PCR, database analysis, double-label immunofluorescence |
The Journal of biological chemistry |
Medium |
12621022
|
| 2003 |
Mouse Chodl (orthologue of human CHODL) is a type I transmembrane C-type lectin expressed in skeletal muscle cells during both proliferation and differentiation phases, as shown by in situ hybridization on E15 embryos and fluorescent immunostaining localizing the protein to striated muscle cells. |
RT-PCR/Southern blotting, in situ hybridization, fluorescent immunostaining, Western blot on C2C12 myoblasts |
Gene |
Medium |
12711387
|
| 2007 |
The soluble CHODL isoforms (CHODLΔe/CHODLfΔe), which lack the transmembrane domain and terminate in a QDEL ER-retention signal, localize to the late endoplasmic reticulum and are expressed in a small lymphocyte population in spleen and tonsils, with differential expression in thymocytes versus peripheral lymphocytes. |
Immunofluorescence localization, immunohistochemistry on tissue sections |
Cell biology international |
Low |
17606388
|
| 2008 |
The cytoplasmic domain of mouse chondrolectin (chodl) directly interacts with the beta-subunit of Rab geranylgeranyl transferase (Rabggtb), identified by SOS recruitment system (SRS) yeast screen and confirmed by in vitro transcription/translation and co-immunoprecipitation. |
SOS recruitment system (SRS) yeast two-hybrid screen, in vitro transcription/translation pulldown, co-immunoprecipitation |
Cellular & molecular biology letters |
Medium |
18161010
|
| 2012 |
In zebrafish, knockdown of chodl causes arrest/stalling of motor axon growth at the horizontal myoseptum (an intermediate target), resulting in reduced muscle innervation; this phenotype is rescued by chodl overexpression, demonstrating that correct chodl expression levels are required for growth cone interactions with the horizontal myoseptum. |
In vivo morpholino knockdown, transgenic overexpression, rescue experiment, in vivo live imaging of labeled motor axons in zebrafish |
The Journal of neuroscience |
High |
22457492
|
| 2013 |
Chodl expression is altered (alternatively spliced) in SMA mouse spinal motor neurons before symptom onset; in vitro studies show Chodl affects cell survival and neurite outgrowth; increasing chodl expression in Smn-depleted zebrafish rescues motor neuron outgrowth defects, linking Chodl dysregulation to SMA motor neuron pathophysiology. |
Exon-level splicing analysis in SMA mouse model, in vitro cell survival and neurite outgrowth assays, in vivo chodl overexpression rescue in smn-knockdown zebrafish |
Human molecular genetics |
High |
24067532
|
| 2011 |
siRNA-mediated knockdown of CHODL suppresses lung cancer cell growth, and exogenous overexpression of CHODL confers growth and invasive activity in mammalian cells, as measured by Matrigel invasion assays. |
siRNA knockdown (cell viability assay), exogenous CHODL overexpression, Matrigel invasion assay |
Clinical cancer research |
Medium |
22016508
|
| 2019 |
CHODL is predicted to undergo S-palmitoylation at a juxtamembrane cysteine residue, identified by a machine-learning topology-driven approach (TopoPalmTree) applied to the mouse transmembrane proteome; experimental assessment of Chodl as a candidate S-palmitoyl substrate was performed (results reported among confirmed candidates). |
Machine-learning prediction (TopoPalmTree), experimental S-palmitoylation assessment (method not fully detailed in abstract) |
The Journal of biological chemistry |
Low |
39909380
|
| 2025 |
Sst-Chodl neurons (somatostatin and chondrolectin co-expressing GABAergic cells) are selectively active during low-arousal states; selective activation of Sst-Chodl cells is sufficient to promote multi-region cortical synchronization and induce sleep via long-range axons targeting multiple neocortical regions simultaneously. |
In vivo electrophysiology/calcium imaging, optogenetic selective activation of Sst-Chodl cells, behavioral sleep assays in mice |
bioRxivpreprint |
Medium |
bio_10.1101_2024.06.20.599756
|
| 2025 |
In a 15q13.3 microdeletion mouse model, single-nucleus transcriptomics showed the largest gene expression alterations in Sst_Chodl neurons; patch-clamp and calcium imaging revealed increased activity specifically in the Sst_Chodl subtype; chemogenetic inhibition of Sst_Chodl neurons rescued sleep disturbances in mutant mice. |
Single-nucleus RNA-seq (snRNA-seq), calcium imaging, patch-clamp electrophysiology, Patch-seq, chemogenetic inhibition (DREADD) with behavioral rescue |
Neuron |
Medium |
40997796
|
| 2025 |
Conditional knockout of Chodl in embryonic myoblasts or adult satellite cells (SCs) does not affect muscle development but markedly impairs regeneration; Chodl-deficient SCs show reduced self-renewal, diminished proliferation, and impaired differentiation; a significant fraction of Chodl-null SCs mislocalize outside the basal lamina and undergo precocious activation, consistent with disrupted ECM and Notch signaling interactions. |
Conditional knockout mouse models (Cre-lox), single-cell RNA-seq, muscle injury regeneration assays, immunofluorescence for SC localization, in silico network perturbation analysis |
iScience |
High |
40964242 42088368
|