Affinage

CFP

Properdin · UniProt P27918

Length
469 aa
Mass
51.3 kDa
Annotated
2026-06-09
100 papers in source corpus 1 papers cited in narrative 1 extracted findings
Cross-family judge vs UniProt: UniProt preferred faithfulness: 1/2 claims corpus-supported (50%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

Complement factor properdin (CFP) has been assigned a tumor-suppressive function in breast cancer, where it acts intracellularly to suppress cell growth both in vitro and in vivo (PMID:30755730). In this context CFP induces intracellular upregulation of the pro-apoptotic transcription factor DDIT3 in association with an endoplasmic reticulum stress response, through a process involving TES (PMID:30755730). Beyond this single low-confidence observation, no further mechanistic detail about the canonical human CFP protein has been characterized in the available corpus.

Mechanistic history

Synthesis pass · year-by-year structured walk · 1 step
  1. 2019 Low

    Whether CFP has an intracellular, growth-regulatory role distinct from complement signaling was unknown; loss- and gain-of-function experiments showed CFP suppresses breast cancer cell growth by inducing the pro-apoptotic factor DDIT3, establishing a candidate tumor-suppressive axis CFP→TES→DDIT3 linked to ER stress.

    Evidence Loss-of-function and gain-of-function screen in MCF7 cells, in vitro and in vivo tumor growth assays, and Western blot for DDIT3

    PMID:30755730

    Open questions at the time
    • Single lab, no reciprocal validation or independent confirmation
    • Mechanism by which CFP engages TES and the molecular link to DDIT3 upregulation is not resolved
    • No structural data and no demonstration of direct physical interactions

Open questions

Synthesis pass · forward-looking unresolved questions
  • How CFP acts intracellularly and whether this role generalizes beyond breast cancer cells remains unresolved.
  • No direct binding partners or substrates established
  • Relationship to canonical extracellular complement function not addressed
  • No structural or biochemical mechanism for DDIT3 induction

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
No controlled-vocabulary terms were assigned to this entry.

Evidence

Reading pass · 1 per-paper finding extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2019 Complement factor properdin (CFP) suppresses breast cancer cell growth in vitro and in vivo by inducing intracellular upregulation of the pro-apoptotic transcription factor DDIT3, associated with endoplasmic reticulum stress response, in a process involving TES. Loss-of-function and gain-of-function screen in MCF7 cell lines; in vitro and in vivo tumor growth assays; Western blot for DDIT3 expression Oncogene Low 30755730

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1998 A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10). Microbiology (Reading, England) 381 9846755
2002 Conclusive evidence that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ESAT-6*CFP-10 complex. Implications for pathogenesis and virulence. The Journal of biological chemistry 270 11940590
2005 Structure and function of the complex formed by the tuberculosis virulence factors CFP-10 and ESAT-6. The EMBO journal 249 15973432
2007 ESAT-6 from Mycobacterium tuberculosis dissociates from its putative chaperone CFP-10 under acidic conditions and exhibits membrane-lysing activity. Journal of bacteriology 246 17557817
2006 Sensitivity of CFP/YFP and GFP/mCherry pairs to donor photobleaching on FRET determination by fluorescence lifetime imaging microscopy in living cells. Microscopy research and technique 135 16941642
2006 The ESAT-6/CFP-10 secretion system of Mycobacterium marinum modulates phagosome maturation. Cellular microbiology 124 16922861
2000 Detection on surfaces and in Caco-2 cells of Campylobacter jejuni cells transformed with new gfp, yfp, and cfp marker plasmids. Applied and environmental microbiology 114 11097924
2020 Quercetin Alleviates LPS-Induced Depression-Like Behavior in Rats via Regulating BDNF-Related Imbalance of Copine 6 and TREM1/2 in the Hippocampus and PFC. Frontiers in pharmacology 111 32009956
2005 Simultaneous imaging of GFP, CFP and collagen in tumors in vivo using multiphoton microscopy. BMC biotechnology 104 15910685
2019 PSD-95 deficiency disrupts PFC-associated function and behavior during neurodevelopment. Scientific reports 102 31263190
2007 A unique Mycobacterium ESX-1 protein co-secretes with CFP-10/ESAT-6 and is necessary for inhibiting phagosome maturation. Molecular microbiology 102 17908204
2000 Molecular and immunological characterization of Mycobacterium tuberculosis CFP-10, an immunodiagnostic antigen missing in Mycobacterium bovis BCG. Journal of clinical microbiology 92 10970372
2003 DsRed as a potential FRET partner with CFP and GFP. Biophysical journal 84 12829514
2007 Mycobacterium tuberculosis secretory proteins CFP-10, ESAT-6 and the CFP10:ESAT6 complex inhibit lipopolysaccharide-induced NF-kappaB transactivation by downregulation of reactive oxidative species (ROS) production. Immunology and cell biology 76 17909563
2010 Engagement of the PFC in consolidation and recall of recent spatial memory. Learning & memory (Cold Spring Harbor, N.Y.) 74 20508034
2004 Use of recombinant ESAT-6:CFP-10 fusion protein for differentiation of infections of cattle by Mycobacterium bovis and by M. avium subsp. avium and M. avium subsp. paratuberculosis. Clinical and diagnostic laboratory immunology 72 15242948
2021 TNF signaling pathway-mediated microglial activation in the PFC underlies acute paradoxical sleep deprivation-induced anxiety-like behaviors in mice. Brain, behavior, and immunity 69 34915154
2023 Oxytocin promotes prefrontal population activity via the PVN-PFC pathway to regulate pain. Neuron 64 37023755
2002 Simultaneous expression of hCNT1-CFP and hENT1-YFP in Madin-Darby canine kidney cells. Localization and vectorial transport studies. The Journal of biological chemistry 62 12097333
2008 Engineering FRET constructs using CFP and YFP. Methods in cell biology 54 18155471
2005 ESAT-6/CFP-10 fusion protein and peptides for optimal diagnosis of mycobacterium tuberculosis infection by ex vivo enzyme-linked immunospot assay in the Gambia. Journal of clinical microbiology 54 15872224
2006 Mycobacterium tuberculosis H37Rv ESAT-6-CFP-10 complex formation confers thermodynamic and biochemical stability. The FEBS journal 53 16689931
2007 Assessment of cross-reactivity between Mycobacterium bovis and M. kansasii ESAT-6 and CFP-10 at the T-cell epitope level. Clinical and vaccine immunology : CVI 50 17671227
2003 A flow cytometric method to detect protein-protein interaction in living cells by directly visualizing donor fluorophore quenching during CFP-->YFP fluorescence resonance energy transfer (FRET). Cytometry. Part A : the journal of the International Society for Analytical Cytology 47 14505312
2020 Medial PFC AMPA receptor and BDNF signaling are required for the rapid and sustained antidepressant-like effects of 5-HT1A receptor stimulation. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 46 32396921
2005 ESAT-6 and CFP-10 in clinical versus environmental isolates of Mycobacterium kansasii. The Journal of infectious diseases 44 15776377
2005 Monokine induced by interferon gamma and IFN-gamma response to a fusion protein of Mycobacterium tuberculosis ESAT-6 and CFP-10 in Brazilian tuberculosis patients. Microbes and infection 44 16269263
2012 Evaluation of two cocktails containing ESAT-6, CFP-10 and Rv-3615c in the intradermal test and the interferon-γ assay for diagnosis of bovine tuberculosis. Preventive veterinary medicine 43 22391021
2012 Immune responses to ESAT-6 and CFP-10 by FASCIA and multiplex technology for diagnosis of M. tuberculosis infection; IP-10 is a promising marker. PloS one 43 23144772
2019 Physical and functional interaction between SET1/COMPASS complex component CFP-1 and a Sin3S HDAC complex in C. elegans. Nucleic acids research 42 31602465
2013 Phenotypic profiling of Mycobacterium tuberculosis EspA point mutants reveals that blockage of ESAT-6 and CFP-10 secretion in vitro does not always correlate with attenuation of virulence. Journal of bacteriology 42 24078612
2011 ESAT-6- and CFP-10-specific Th1, Th22 and Th17 cells in tuberculous pleurisy may contribute to the local immune response against Mycobacterium tuberculosis infection. Scandinavian journal of immunology 42 21223348
2008 Pharmacological and therapeutic directions in ADHD: Specificity in the PFC. Behavioral and brain functions : BBF 42 18304369
2008 CFP and YFP, but not GFP, provide stable fluorescent marking of rat hepatic adult stem cells. Journal of biomedicine & biotechnology 42 18401450
2011 Effects of Mycobacterium tuberculosis ESAT-6/CFP-10 fusion protein on the autophagy function of mouse macrophages. DNA and cell biology 41 21740189
2011 Agomelatine (S20098) modulates the expression of cytoskeletal microtubular proteins, synaptic markers and BDNF in the rat hippocampus, amygdala and PFC. Psychopharmacology 41 22160164
2006 Detecting protein-protein interactions with CFP-YFP FRET by acceptor photobleaching. Current protocols in cytometry 41 18770833
2001 High resolution, fluorescence deconvolution microscopy and tagging with the autofluorescent tracers CFP, GFP, and YFP to study the structural composition of gap junctions in living cells. Microscopy research and technique 40 11180618
2010 Humoral immune responses against the Mycobacterium tuberculosis 38-kilodalton, MTB48, and CFP-10/ESAT-6 antigens in tuberculosis. Clinical and vaccine immunology : CVI 39 20053875
2007 High-precision FLIM-FRET in fixed and living cells reveals heterogeneity in a simple CFP-YFP fusion protein. Biophysical chemistry 39 17336446
2004 Characterisation of complex formation between members of the Mycobacterium tuberculosis complex CFP-10/ESAT-6 protein family: towards an understanding of the rules governing complex formation and thereby functional flexibility. FEMS microbiology letters 39 15336430
2017 Adolescent social isolation affects schizophrenia-like behavior and astrocyte biomarkers in the PFC of adult rats. Behavioural brain research 38 28705472
2004 Evaluation of the CFP-substrate-YFP system for protease studies: advantages and limitations. BioTechniques 38 15152609
2019 Detection of Mycobacterium tuberculosis lipoarabinomannan and CFP-10 (Rv3874) from urinary extracellular vesicles of tuberculosis patients by immuno-PCR. Pathogens and disease 37 31549171
2009 Development of the transgenic cyan fluorescent protein (CFP)-expressing nude mouse for "Technicolor" cancer imaging. Journal of cellular biochemistry 37 19306297
2005 Determination of tumor necrosis factor receptor-associated factor trimerization in living cells by CFP->YFP->mRFP FRET detected by flow cytometry. Nucleic acids research 36 15805120
2016 A solar light driven dual photoelectrode photocatalytic fuel cell (PFC) for simultaneous wastewater treatment and electricity generation. Journal of hazardous materials 34 26954476
2019 Pathoetiology and pathophysiology of borderline personality: Role of prenatal factors, gut microbiome, mu- and kappa-opioid receptors in amygdala-PFC interactions. Progress in neuro-psychopharmacology & biological psychiatry 33 31689444
2010 The development and preliminary evaluation of a new Mycobacterium tuberculosis vaccine comprising Ag85b, HspX and CFP-10:ESAT-6 fusion protein with CpG DNA and aluminum hydroxide adjuvants. FEMS immunology and medical microbiology 32 20298499
2013 Photocatalytic fuel cell (PFC) and dye self-photosensitization photocatalytic fuel cell (DSPFC) with BiOCl/Ti photoanode under UV and visible light irradiation. Environmental science & technology 31 23472666
2013 IgA response to ESAT-6/CFP-10 and Rv2031 antigens varies in patients with culture-confirmed pulmonary tuberculosis, healthy Mycobacterium tuberculosis-infected and non-infected individuals in a tuberculosis endemic setting, Ethiopia. Scandinavian journal of immunology 31 23713613
2014 CFP-10 from Mycobacterium tuberculosis selectively activates human neutrophils through a pertussis toxin-sensitive chemotactic receptor. Infection and immunity 30 25332123
2019 CFP suppresses breast cancer cell growth by TES-mediated upregulation of the transcription factor DDIT3. Oncogene 29 30755730
2012 Specificity of the tuberculin skin test is modified by use of a protein cocktail containing ESAT-6 and CFP-10 in cattle naturally infected with Mycobacterium bovis. Clinical and vaccine immunology : CVI 29 22419675
2009 Descending glutamatergic pathways of PFC are involved in acute and chronic action of methylphenidate. Brain research 29 19747456
2005 CaMK-II oligomerization potential determined using CFP/YFP FRET. Biochimica et biophysica acta 29 16185778
2015 Quantitative analysis of recombination between YFP and CFP genes of FRET biosensors introduced by lentiviral or retroviral gene transfer. Scientific reports 28 26290434
2003 Structure determination, apoptosis induction, and telomerase inhibition of CFP-2, a novel lichenin from Cladonia furcata. Biochimica et biophysica acta 28 12880947
2011 Novel monoclonal antibodies to ESAT-6 and CFP-10 antigens for ELISA-based diagnosis of pleural tuberculosis. The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease 27 21575303
2010 Rapid identification of the Mycobacterium tuberculosis complex by combining the ESAT-6/CFP-10 immunochromatographic assay and smear morphology. Journal of clinical microbiology 27 21159936
2014 Prevention of alcohol-heightened aggression by CRF-R1 antagonists in mice: critical role for DRN-PFC serotonin pathway. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 26 24917195
2009 Cyan fluorescent protein (CFP) expressing cells in the retina of Thy1-CFP transgenic mice before and after optic nerve injury. Neuroscience letters 26 19879331
2018 Chronic noise exposure exacerbates AD-like neuropathology in SAMP8 mice in relation to Wnt signaling in the PFC and hippocampus. Scientific reports 24 30279527
2016 Baclofen ameliorates spatial working memory impairments induced by chronic cerebral hypoperfusion via up-regulation of HCN2 expression in the PFC in rats. Behavioural brain research 24 27085590
2021 miR-132 downregulation alleviates behavioral impairment of rats exposed to single prolonged stress, reduces the level of apoptosis in PFC, and upregulates the expression of MeCP2 and BDNF. Neurobiology of stress 23 33718536
2016 Fc-based delivery system enhances immunogenicity of a tuberculosis subunit vaccine candidate consisting of the ESAT-6:CFP-10 complex. Molecular bioSystems 23 27138226
2000 Sensitivity of the SRBC PFC assay versus ELISA for detection of immunosuppression by TCDD and TCDD-like congeners. Toxicology 23 11162871
2011 Association of TMEM18 variants with BMI and waist circumference in children and correlation of mRNA expression in the PFC with body weight in rats. European journal of human genetics : EJHG 22 21952719
2022 FAAH Inhibition Restores Early Life Stress-Induced Alterations in PFC microRNAs Associated with Depressive-Like Behavior in Male and Female Rats. International journal of molecular sciences 21 36555739
2020 Detection of mycobacterial CFP-10 (Rv3874) protein in tuberculosis patients by gold nanoparticle-based real-time immuno-PCR. Future microbiology 20 32490745
2013 Melanopsin-expressing retinal ganglion cell loss and behavioral analysis in the Thy1-CFP-DBA/2J mouse model of glaucoma. Science China. Life sciences 20 23729182
2010 Suite of novel vectors for ectopic insertion of GFP, CFP and IYFP transcriptional fusions in single copy at the amyE and bglS loci in Bacillus subtilis. Plasmid 20 20600285
2014 Optimization and application of TiO₂/Ti-Pt photo fuel cell (PFC) to effectively generate electricity and degrade organic pollutants simultaneously. Water research 19 24926902
2000 Isolation, purification and immunological characterization of novel low molecular weight protein antigen CFP 6 from culture filtrate of M. tuberculosis. Vaccine 19 10812229
2021 Estrogen Receptors Alpha and Beta Mediate Synaptic Transmission in the PFC and Hippocampus of Mice. International journal of molecular sciences 18 33540803
2021 Sustained NMDA receptor hypofunction impairs brain-derived neurotropic factor signalling in the PFC, but not in the hippocampus, and disturbs PFC-dependent cognition in mice. Journal of psychopharmacology (Oxford, England) 18 34008450
2013 Multiple effects of β-amyloid on single excitatory synaptic connections in the PFC. Frontiers in cellular neuroscience 18 24027495
2007 Interferon gamma response to combinations 38 kDa/CFP-10, 38 kDa/MPT-64, ESAT-6/MPT-64 and ESAT-6/CFP-10, each related to a single recombinant protein of Mycobacterium tuberculosis in individuals from tuberculosis endemic areas. Microbiology and immunology 18 17380048
1986 Comparison of accessory cell functions of endothelial cells and monocytes: IL-2 production by T cells and PFC generation. Cellular immunology 18 3488818
2023 Blunt dopamine transmission due to decreased GDNF in the PFC evokes cognitive impairment in Parkinson's disease. Neural regeneration research 17 36255000
2018 Region-Dependent Alterations in Cognitive Function and ERK1/2 Signaling in the PFC in Rats after Social Defeat Stress. Neural plasticity 17 29849577
2017 Stress-altered synaptic plasticity and DAMP signaling in the hippocampus-PFC axis; elucidating the significance of IGF-1/IGF-1R/CaMKIIα expression in neural changes associated with a prolonged exposure therapy. Neuroscience 17 28438613
2023 Cannabidiol Modulates Alterations in PFC microRNAs in a Rat Model of Depression. International journal of molecular sciences 16 36768376
2014 Measurement of phenotype and absolute number of circulating heparin-binding hemagglutinin, ESAT-6 and CFP-10, and purified protein derivative antigen-specific CD4 T cells can discriminate active from latent tuberculosis infection. Clinical and vaccine immunology : CVI 16 25520147
2001 Over-expression of the cercosporin facilitator protein, CFP, in Cercospora kikuchii up-regulates production and secretion of cercosporin. FEMS microbiology letters 16 11682184
2023 Development of Gold-Nanoparticle-Based Lateral Flow Immunoassays for Rapid Detection of TB ESAT-6 and CFP-10. Biosensors 15 36979566
2018 Evaluation of photocatalytic fuel cell (PFC) for electricity production and simultaneous degradation of methyl green in synthetic and real greywater effluents. Journal of environmental management 15 30243074
2016 Macrophage Immune Response Suppression by Recombinant Mycobacterium tuberculosis Antigens, the ESAT-6, CFP-10, and ESAT-6/CFP-10 Fusion Proteins. Iranian journal of medical sciences 15 27365551
2011 Quantitative measurement of Ca(2+)-dependent calmodulin-target binding by Fura-2 and CFP and YFP FRET imaging in living cells. Biochemistry 15 21517110
2021 Distinct Role of Dopamine in the PFC and NAc During Exposure to Cocaine-Associated Cues. The international journal of neuropsychopharmacology 14 34626116
2017 Association of ESAT-6/CFP-10-induced IFN-γ, TNF-α and IL-10 with clinical tuberculosis: evidence from cohorts of pulmonary tuberculosis patients, household contacts and community controls in an endemic setting. Clinical and experimental immunology 14 28374535
2015 Imaging the microenvironment of pancreatic cancer patient-derived orthotopic xenografts (PDOX) growing in transgenic nude mice expressing GFP, RFP, or CFP. Cancer letters 14 26742463
2013 Limited T cell receptor beta variable repertoire responses to ESAT-6 and CFP-10 in subjects infected with Mycobacterium tuberculosis. Tuberculosis (Edinburgh, Scotland) 14 23845455
2011 Expression and Purification of Recombinant Mycobacterium Tuberculosis (TB) Antigens, ESAT-6, CFP-10 and ESAT- 6/CFP-10 and Their Diagnosis Potential for Detection of TB Patients. Iranian Red Crescent medical journal 14 22737527
2004 The Neurospora crassa cfp promoter drives a carbon source-dependent expression of transgenes in filamentous fungi. Journal of applied microbiology 14 15139917
2023 The Antidepressant-like Activity and Cognitive Enhancing Effects of the Combined Administration of (R)-Ketamine and LY341495 in the CUMS Model of Depression in Mice Are Related to the Modulation of Excitatory Synaptic Transmission and LTP in the PFC. Pharmaceuticals (Basel, Switzerland) 13 37083635
2020 Network preservation reveals shared and unique biological processes associated with chronic alcohol abuse in NAc and PFC. PloS one 13 33332381
2019 Distribution and expression of esat-6 and cfp-10 in non-tuberculous mycobacteria isolated from lymph nodes of slaughtered cattle in Switzerland. Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 13 30636533
2013 Cloning, expression and purification of Mycobacterium tuberculosis ESAT-6 and CFP-10 antigens. Iranian journal of microbiology 13 25848507
2007 ESAT-6 and CFP-10 can be combined to reduce the cost of testing for Mycobacterium tuberculosis infection, but CFP-10 responses associate with active disease. Transactions of the Royal Society of Tropical Medicine and Hygiene 13 17434194

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