Affinage

CD177

CD177 antigen · UniProt Q8N6Q3

Length
437 aa
Mass
46.4 kDa
Annotated
2026-06-09
100 papers in source corpus 28 papers cited in narrative 27 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CD177 (NB1/PRV-1) is a GPI-anchored cell-surface glycoprotein of the uPAR/CD59/Ly-6 snake-toxin superfamily, expressed on a genetically and epigenetically restricted subset of neutrophils where it governs neutrophil adhesion, transendothelial migration, and presentation of the autoantigen proteinase 3 (PR3) (PMID:11465086, PMID:17244676). Biochemically it is a ~50 kDa N-glycosylated, GPI-linked protein with two cysteine-rich domains that is released from the membrane by PI-PLC and stored in secondary granules and secretory vesicles (PMID:1825110, PMID:2153425, PMID:10753836, PMID:12239154). CD177 serves as a heterophilic counter-receptor for endothelial PECAM-1 (engaging PECAM-1 domain 6 in a cation-dependent manner), an interaction that supports neutrophil diapedesis and is tuned by the endothelial PECAM-1 S536N dimorphism through modulation of PECAM-1 ITIM and β-catenin phosphorylation (PMID:17580308, PMID:20194726). It functions as the membrane receptor that presents catalytically active PR3 on the neutrophil surface via a discrete hydrophobic cluster in PR3, and assembles into a lipid-raft signaling complex with Mac-1 (CD11b/CD18) that transduces PR3-ANCA-mediated degranulation and oxidative burst (PMID:17244676, PMID:18854317, PMID:21193407). Upon ligation, CD177 enhances its interaction with β2 integrins to drive Src/ERK signaling, increase integrin affinity, and arrest migration, while PECAM-1 engagement conversely downregulates membrane PR3 and dampens ANCA-induced activation (PMID:28807980, PMID:30236159). Subset-restricted expression is established by monoallelic transcription enforced by promoter CpG methylation with c-Jun/c-Fos binding to the active allele, and the CD177null phenotype arises from pseudogene-derived allelic gene conversion introducing a stop codon (PMID:28559244, PMID:27227454). Beyond neutrophils, CD177 marks immunosuppressive tumor-infiltrating Tregs, modulates Wnt/β-catenin signaling in mammary epithelium, and partners with podoplanin on cancer-associated fibroblasts (PMID:34599187, PMID:32042113, PMID:34879110). CD177+ neutrophil subsets drive context-specific protective or pathogenic functions in colitis, biliary atresia, and lung ischemia-reperfusion injury, the latter two via mitochondrial-ROS-driven NET formation (PMID:28468761, PMID:35803543, PMID:40398393).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 1991 High

    Established the basic biochemical nature of the NB1 antigen, defining it as a GPI-anchored membrane glycoprotein rather than a transmembrane protein.

    Evidence PI-PLC release of surface-labeled neutrophil protein with non-denaturing gel shift and endoglycosidase digestion

    PMID:1825110 PMID:2153425

    Open questions at the time
    • Protein sequence and gene identity not yet determined
    • Function unknown
  2. 2001 High

    Cloned the gene and placed CD177 in the uPAR/CD59/Ly-6 superfamily, defining its domain architecture and confirming surface expression in transfected cells.

    Evidence Immunoaffinity purification, MALDI-TOF MS, N-terminal sequencing, cDNA cloning, COS-7/transfection FACS (also molecular cloning of PRV-1)

    PMID:10753836 PMID:11465086 PMID:12239154

    Open questions at the time
    • No ligand or receptor function identified
    • Reason for restricted neutrophil expression unknown
  3. 2007 High

    Identified two distinct binding functions of CD177 — as the counter-receptor for endothelial PECAM-1 and as the membrane receptor presenting PR3 — establishing its dual role in migration and autoantigen display.

    Evidence SPR, reciprocal immunoprecipitation, adhesion/transmigration assays for PECAM-1; co-IP, co-localization, GPI removal, and transfection rescue for PR3

    PMID:17244676 PMID:17580308

    Open questions at the time
    • Molecular determinants of PR3 binding not yet mapped
    • Downstream signaling partners unknown
  4. 2008 High

    Mapped the PR3 binding determinant to a species-specific hydrophobic cluster, demonstrating molecular specificity of the CD177-PR3 interaction.

    Evidence Human-gibbon PR3 hybrid domain swaps with NB1-binding and activity assays

    PMID:18854317

    Open questions at the time
    • The reciprocal CD177 binding interface not defined
    • Structural model absent
  5. 2010 High

    Resolved how a GPI-anchored protein transduces signals by identifying Mac-1 as the transmembrane adaptor in the CD177-PR3 raft complex that drives ANCA-mediated activation.

    Evidence Membrane complex IP with MS/MS, SPR, Mac-1-transfected adhesion assay, lipid raft fractionation, blocking-antibody degranulation/oxidative burst assays

    PMID:21193407

    Open questions at the time
    • Stoichiometry of the CD177/PR3/Mac-1 complex not defined
    • Whether other integrins substitute in vivo unclear
  6. 2012 Medium

    Refined the migration mechanism by showing PR3 serine protease activity itself facilitates CD177+ neutrophil diapedesis and that the CD177/PECAM-1 axis is migration-specific, not platelet-binding.

    Evidence Transendothelial migration with serine protease inhibitors and PR3 activity measurement; platelet-neutrophil conjugate assays under flow

    PMID:22266279 PMID:22690867

    Open questions at the time
    • Substrates cleaved by PR3 during diapedesis not identified
    • Single-lab functional data
  7. 2017 High

    Established the integrin-coupled signaling output of CD177 ligation and the epigenetic basis of subset restriction, explaining both how CD177 controls migration and why only some neutrophils express it.

    Evidence FLIM of CD177-β2 integrin interaction with Src/ERK phosphorylation and integrin affinity assays; single-cell haplotype analysis, ChIP for c-Jun/c-Fos and histone marks, CpG methylation and reporter assays

    PMID:28559244 PMID:28807980

    Open questions at the time
    • Trigger for allele choice during differentiation unknown
    • Physiological ligand initiating CD177 ligation in vivo not defined
  8. 2016 Medium

    Defined the genetic basis of the CD177null phenotype, attributing it to pseudogene-derived gene conversion and heritable control of CD177hi neutrophil proportion.

    Evidence Deep sequencing of the CD177 locus with family and population genotype-phenotype correlation

    PMID:12623849 PMID:27227454

    Open questions at the time
    • Mechanism of allelic gene conversion not detailed
    • Population frequency of conversion event unclear
  9. 2017 High

    Demonstrated context-dependent roles of CD177+ neutrophils in tissue protection and the inhibitory tuning of mPR3 by PECAM-1, linking CD177 biology to inflammatory disease.

    Evidence CD177 KO mice in DSS colitis with subset RNA-seq and functional assays; recombinant PECAM-1 mPR3 downregulation assays with CD177-negative controls (also 2014 KO survival phenotype)

    PMID:25359465 PMID:28468761 PMID:30236159

    Open questions at the time
    • Molecular link between CD177 and IL-22/survival programs unclear
    • Whether protective vs pathogenic balance is tissue-intrinsic unknown
  10. 2021 Medium

    Extended CD177 function beyond neutrophils, identifying it on suppressive tumor-infiltrating Tregs, in epithelial Wnt regulation, in endoderm fate, and as a podoplanin partner on CAFs.

    Evidence scRNA-seq plus conditional Treg KO tumor model; breast cancer loss-of-function with β-catenin readout; hPSC endoderm sorting/differentiation; PDPN surface interactome screen with phosphoproteomics

    PMID:32042113 PMID:32341565 PMID:34599187 PMID:34879110

    Open questions at the time
    • Mechanism linking GPI-anchored CD177 to intracellular Wnt/β-catenin unresolved
    • Direct vs indirect role in Treg suppression unclear
    • Single-lab findings each
  11. 2025 Medium

    Defined a metabolic axis in pathogenic CD177+ neutrophils, showing mitochondrial complex I activity drives NET-mediated tissue injury in biliary atresia and lung ischemia-reperfusion.

    Evidence scRNA-seq/spatial transcriptomics, complex I activity assays, NET quantification, and pharmacological complex I inhibition in mouse and rat models

    PMID:35803543 PMID:40398393

    Open questions at the time
    • Whether CD177 itself regulates mitochondrial metabolism vs marks metabolically active cells unclear
    • Single-lab models

Open questions

Synthesis pass · forward-looking unresolved questions
  • How a GPI-anchored Ly-6 protein with no intrinsic catalytic or transmembrane domain mechanistically couples to intracellular Wnt/β-catenin and metabolic programs across diverse non-neutrophil cell types remains unresolved.
  • No structural model of CD177 or its complexes
  • Signal transduction in epithelial/Treg/endoderm contexts not mechanistically defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 2 GO:0060089 molecular transducer activity 2 GO:0098631 cell adhesion mediator activity 2
Localization
GO:0005886 plasma membrane 3 GO:0031410 cytoplasmic vesicle 2
Pathway
R-HSA-1500931 Cell-Cell communication 2 R-HSA-162582 Signal Transduction 2 R-HSA-168256 Immune System 2
Partners
ITGAMITGB2PDPNPECAM-1PRTN3
Complex memberships
CD177/PR3/Mac-1 lipid-raft signaling complex

Evidence

Reading pass · 27 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1991 The NB1 (CD177) antigen is anchored to the neutrophil plasma membrane via a glycosyl-phosphatidylinositol (GPI) linkage, as demonstrated by release of the 58–64 kDa protein from the cell surface upon treatment with phosphatidylinositol-specific phospholipase C (PI-PLC) and shift in electrophoretic mobility under non-denaturing conditions. PI-PLC treatment of 125I-surface-labeled neutrophils, non-denaturing gel electrophoresis, endoglycosidase-F digestion Journal of leukocyte biology High 1825110
1990 The NB1 (CD177) antigen resides on a 58–64 kDa surface glycoprotein that is also present in secondary (but not primary) neutrophil granules; the NB1 epitope is protein-based (not carbohydrate), requires intact tertiary structure (disulfide bonds), and is lost upon reduction. SDS-PAGE, immunoblotting with alloantisera, immunoprecipitation of 125I-labeled neutrophil membranes and granule fractions prepared by nitrogen cavitation and differential centrifugation; periodate and reduction treatments Blood High 2153425
2001 The NB1 (CD177) glycoprotein is a ~50.6 kDa GPI-anchored glycoprotein (43 kDa after N-glycan removal) belonging to the uPAR/CD59/Ly-6 snake-toxin superfamily, with two cysteine-rich domains, three N-linked glycosylation sites, and a GPI attachment site; surface expression was confirmed in transfected COS-7 cells. Immunoaffinity chromatography, MALDI-TOF mass spectrometry, N-terminal amino acid sequencing, RACE-PCR, cDNA cloning, COS-7 transfection with flow cytometry European journal of immunology High 11465086
2000 PRV-1 (CD177) encodes a GPI-anchored cell-surface receptor of 437 amino acids with two cysteine-rich uPAR/Ly6 superfamily domains; the protein is N-glycosylated, shed into cell supernatants as a soluble form, and expressed specifically on myeloid/granulocytic cells; overexpression in polycythemia vera is not due to gene rearrangement. Subtractive hybridization, Northern blot, cDNA cloning, intron-exon structure determination, FACS of stably transfected cells, immunoblotting, flow cytometry of bone marrow and peripheral blood Blood High 10753836 12239154
2007 CD177 (NB1) is a heterophilic counter-receptor for platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) on endothelial cells; the interaction is cation-dependent and involves PECAM-1 domain 6; blocking antibodies to CD177 or PECAM-1 domain 6 inhibit both cell adhesion and transendothelial migration of neutrophils. Flow cytometry, immunoprecipitation, surface plasmon resonance (real-time protein–protein interaction analysis), U937 cell adhesion assays with immobilized PECAM-1, transendothelial migration assays with blocking monoclonal antibodies The Journal of biological chemistry High 17580308
2007 NB1 (CD177) mediates surface expression of the autoantigen proteinase 3 (PR3) on human neutrophils; PR3 and NB1 co-immunoprecipitate and co-localize on the plasma membrane; enzymatic removal of GPI anchors reduces membrane PR3 expression; transfection of NB1 into HEK293 and HL60 cells confers specific surface PR3 binding. Flow cytometry, confocal microscopy, co-immunoprecipitation, Western blot, GPI anchor removal with phospholipase, transfection of HEK293 and HL60 cells, magnetic cell sorting of NB1+/NB1− subsets, cord blood stem cell differentiation model Blood High 17244676
2006 CD177 and membrane PR3 are co-expressed exclusively on the same neutrophil subset; their surface expression increases or decreases in parallel during cell stimulation or apoptosis; both undergo rapid internalization and recirculation (all mPR3 replaced within 30 min), and their co-expression indicates shared storage in secondary granules and secretory vesicles (not primary granules). Flow cytometry of matched CD177/mPR3 expression, stimulation and apoptosis assays, kinetic internalization experiments Journal of leukocyte biology Medium 17077162
2008 A unique hydrophobic cluster in human PR3 (residues F166, W218, G219, L223 in the C-terminal half) mediates binding to the NB1 (CD177) receptor on the cell surface; the gibbon PR3 homolog, which lacks these residues, does not bind human NB1; NB1-bound PR3 retains enzymatic activity and is cleared from the surface by α1-protease inhibitor. Cloning of gibbon PR3 homolog, construction of human–gibbon PR3 hybrid proteins, NB1-binding assays, activity assays with α1-protease inhibitor The Journal of biological chemistry High 18854317
2010 Mac-1 (CD11b/CD18) is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation: the NB1 signaling complex precipitated from plasma membranes contains Mac-1, as identified by MS/MS and immunoblotting; NB1 shows direct protein–protein interaction with CD11b and CD11a by SPR, but only Mac-1-transfected cells adhere to immobilized NB1; NB1, PR3, and Mac-1 reside in lipid rafts; CD11b blockade inhibits NB1-triggered degranulation and superoxide production. Membrane complex immunoprecipitation, MS/MS and immunoblotting identification of Mac-1, surface plasmon resonance, cell adhesion assays on transfected cells, lipid raft fractionation, confocal microscopy co-localization, functional assays (degranulation, oxidative burst) with blocking antibodies The Journal of biological chemistry High 21193407
2010 CD177-mediated neutrophil transmigration is influenced by the endothelial PECAM-1 S536N dimorphism: CD177+ neutrophils migrate significantly faster through HUVECs expressing the LSR allelic variant than the VNG variant; heterophilic CD177/PECAM-1 engagement suppresses PECAM-1 ITIM tyrosine phosphorylation and increases β-catenin phosphorylation, facilitating junction opening in an allele-specific manner. In vitro transendothelial migration assays through HUVECs expressing defined PECAM-1 allelic variants, rCD177-Fc engagement, phosphorylation assays for PECAM-1 ITIM and β-catenin Journal of immunology Medium 20194726
2012 PR3 serine protease activity facilitates transendothelial migration specifically of NB1 (CD177)-positive neutrophils: PR3 expression and activity increase on NB1+ neutrophils following transmigration (not on NB1− neutrophils); selective serine protease inhibitors block NB1+ neutrophil diapedesis under static and flow conditions. Transendothelial migration assays through IL-1β-stimulated endothelial monolayers, blocking antibodies to PECAM-1, PR3 activity measurement after transmigration, selective serine protease inhibitors under static and flow conditions Journal of immunology Medium 22266279
2017 CD177 ligation on CD177pos neutrophils selectively impairs their migration by immobilizing cells and causing spreading; mechanistically, CD177 ligation enhances its interaction with β2 integrins (shown by fluorescence lifetime imaging microscopy), leading to integrin-mediated phosphorylation of Src and ERK, increased surface β2 integrin expression and affinity, impaired integrin attachment internalization, and ERK-mediated attenuation of chemokine signaling. PECAM-1-independent transwell migration assay, fluorescence lifetime imaging microscopy (FLIM) for CD177–β2 integrin interaction, phosphorylation assays (Src, ERK), surface integrin expression and affinity measurements, chemokine receptor internalization assays, kinase inhibitor studies Blood High 28807980
2017 Subset-restricted CD177 expression in bimodal individuals arises from monoallelic expression: CD177pos neutrophils transcribe only the maternal or paternal allele, while the other allele is silenced during neutrophil differentiation from hematopoietic stem cells that express both alleles; DNA methylation of the CD177 promoter reduces expression, demethylation causes biallelic expression, and c-Jun/c-Fos binding to the unmethylated, euchromatic promoter drives CD177 transcription in CD177pos neutrophils. Single-cell haplotype analysis, ChIP for histone marks and c-Jun/c-Fos, CpG methylation analysis, reporter assays in HeLa cells with mono- vs. biallelic expression, demethylation experiments, hematopoietic stem cell differentiation tracking The Journal of experimental medicine High 28559244
2003 NB1-negative neutrophils in bimodal individuals contain no NB1 mRNA even after G-CSF stimulation, and this transcriptional silencing correlates with specific CD177 coding polymorphisms; three out of six identified polymorphisms are significantly associated with a small NB1-expressing neutrophil subpopulation. Immunofluorescence-based single-cell picking and FACS sorting of NB1+ vs NB1− subsets, RT-PCR for NB1 mRNA, cDNA sequencing, G-CSF stimulation experiments Blood Medium 12623849
2016 The CD177null phenotype arises when exon 7 of the CD177 gene is supplied entirely by the CD177P1 pseudogene through allelic gene conversion, introducing a stop codon; the proportion of CD177hi neutrophils in blood is heritable and correlates with CD177 reference allele homozygosity. Deep sequencing of the CD177 locus, identification of stop codon from pseudogene-derived exon 7 sequence, genotype–phenotype correlation in families and population cohorts PLoS genetics Medium 27227454
2014 CD177 deletion in mice reduces peripheral blood neutrophil counts and reduces neutrophil accumulation in S. aureus skin infection; CD177 deletion has no significant impact on CXCL1/KC- or fMLP-induced neutrophil migration but leads to significant neutrophil cell death, indicating a pro-survival role. CD177 genetic knockout mouse model, complete blood counts, S. aureus skin infection model with neutrophil counting, CXCL1/fMLP migration assays, cell death assays Protein & cell Medium 25359465
2017 CD177+ neutrophils in IBD produce lower levels of pro-inflammatory cytokines (IFN-γ, IL-6, IL-17A) but higher levels of IL-22 and TGF-β, exhibit increased bactericidal activities (ROS, antimicrobial peptides, NETs), and protect against colitis; CD177−/− mice develop more severe DSS colitis with compromised intestinal barrier and impaired IL-22 production. Flow cytometry and immunohistochemistry in IBD patients, RNA sequencing of sorted CD177+/CD177− neutrophil subsets, cytokine/ROS/NET functional assays, DSS colitis model in CD177−/− mice Gut High 28468761
2021 CD177 is expressed on a specific Fate-1 subset of tumor-infiltrating regulatory T cells (TI Tregs) in renal clear cell carcinoma and other solid cancers; blocking CD177 reduces Treg suppressive activity in vitro, and Treg-specific deletion of Cd177 in mice leads to decreased tumor growth and reduced TI Treg frequency. Single-cell RNA sequencing of ccRCC immune cells, in vitro Treg suppression assay with CD177 blocking, conditional Treg-specific Cd177 knockout mouse tumor model Nature communications Medium 34599187
2020 CD177 marks anterior definitive endoderm (ADE) subpopulations in human pluripotent stem cell-derived cultures; CD177+ ADE expresses and synthesizes the secreted WNT/NODAL/BMP antagonist CERBERUS1 and is specified toward pancreatic fate through non-canonical WNT signaling, whereas CD275+ ADE receives canonical WNT signaling and is specified toward liver fate; isolated CD177+ ADE differentiates more homogeneously into functional glucose-responsive β-like cells. Surface marker-based sorting of definitive endoderm subpopulations, RNA-seq and signaling pathway analysis, CERBERUS1 expression/secretion assays, in vitro pancreatic and hepatic differentiation with functional glucose-stimulated insulin secretion assays Nature biotechnology Medium 32341565
2018 CD177 is a functional binding partner of podoplanin (PDPN) on cancer-associated fibroblasts, identified via high-throughput surface interactome screening; CD177 acts as a functional antagonist of PDPN, recapitulating the phenotype of PDPN-deficient CAFs, and PDPN signaling involves actomyosin contractility and cell growth pathways. High-throughput PDPN cell surface interactome screen, quantitative phosphoproteomics of CAF phosphoproteome, cellular phenotype assays in PDPN-deficient and CD177-treated CAFs PloS one Medium 34879110
2020 CD177 deficiency in breast cancer cells is associated with increased β-catenin/Wnt signaling; loss of CD177 leads to hyperproliferative mammary epithelium; CD177 acts as a novel regulator of mammary epithelial proliferation through modulation of the Wnt/β-catenin pathway. CD177 knockout/knockdown in breast cancer cell lines and mouse mammary gland models, β-catenin signaling pathway analysis, proliferation assays Oncogene Medium 32042113
2018 PECAM-1 interaction with CD177 downregulates membrane PR3 (mPR3) expression on neutrophils in a dose-dependent manner; this results in attenuated PR3-ANCA-induced neutrophil degranulation and oxidative burst; the inhibitory effect on degranulation is absent in CD177-negative neutrophils, confirming CD177 dependence. ELISA and flow cytometry for mPR3 expression, DHR oxidative burst assay, degranulation ELISA, MACS-sorted CD177-negative neutrophil experiments with recombinant PECAM-1 Arthritis research & therapy Medium 30236159
2012 CD177/PECAM-1 adhesive interaction supports neutrophil transendothelial migration but does not contribute to platelet–neutrophil interactions: CD177+ and CD177− neutrophils do not differ in platelet conjugate formation under static or flow conditions, whereas predominantly CD177+ neutrophils migrate preferentially across HUVEC monolayers; the PECAM-1 S536N dimorphism affects transendothelial migration but not platelet binding. Whole-blood and isolated platelet–neutrophil conjugate assays under flow and static conditions, transendothelial migration across HUVEC monolayers, comparison of PECAM-1 allelic variants European journal of haematology Medium 22690867
1994 Unstimulated NB1-positive neutrophils show reduced adherence to HUVEC monolayers compared to NB1-negative neutrophils; blocking NB1 with Fab fragments increases NB1+ neutrophil adherence to HUVEC; NB1+ neutrophils show greater chemotaxis to fMLP through nitrocellulose but not polycarbonate membranes; no differences in adhesion to extracellular matrix proteins. Adhesion assays of sorted NB1+/NB1− neutrophils to HUVEC monolayers and ECM proteins, anti-NB1 Fab fragment blocking, chemotaxis assays through nitrocellulose and polycarbonate membranes The Journal of laboratory and clinical medicine Medium 8301201
2010 In ANCA-associated vasculitis and SLE, the percentage of CD177-expressing neutrophils is significantly increased, but primed CD177-negative neutrophils from CD177-null donors also express mPR3 and are susceptible to anti-PR3-mediated oxidative burst, indicating that a CD177-independent pathway for mPR3 membrane recruitment exists. Flow cytometry of neutrophils from ASV, SLE, RA patients and healthy controls, DHR oxidative burst assay, TNF-α priming, comparison of CD177-null donor and sorted CD177− subsets Arthritis and rheumatism Medium 19404956
2022 CD177+ neutrophils are the main Gr-1+ population in mouse biliary atresia livers; they exhibit elevated mitochondrial ROS and form neutrophil extracellular traps (NETs) that induce apoptosis of biliary epithelial cells in co-culture; Cd177−/− mice show delayed disease onset and reduced morbidity/mortality; NET inhibitors reduce bile duct damage. scRNA-seq and Smart-Seq RNA-seq of liver Gr-1+ cells, Cd177−/− mouse BA model (rhesus rotavirus), ROS measurement, NET quantification, co-culture apoptosis assay, NET inhibitor treatment, pilot clinical study with N-acetylcysteine Journal of hepatology High 35803543
2025 CD177+ neutrophils in lung ischemia-reperfusion injury exhibit elevated oxidative phosphorylation and increased mitochondrial complex I activity, driving inflammation and NET formation; inhibiting complex I with IACS-010759 reduces CD177+ neutrophil activation and alleviates lung injury in mouse IRI and rat lung transplant models. scRNA-seq and spatial transcriptomics of IRI lung, mitochondrial complex I activity assays, IACS-010759 pharmacological inhibition in mouse IRI and rat transplant models, NET quantification, human lung transplant cohort AUC analysis Cell reports. Medicine Medium 40398393

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2017 CD177+ neutrophils as functionally activated neutrophils negatively regulate IBD. Gut 223 28468761
2007 The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31). The Journal of biological chemistry 197 17580308
2000 Cloning of PRV-1, a novel member of the uPAR receptor superfamily, which is overexpressed in polycythemia rubra vera. Blood 171 10753836
2017 CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation. Blood 152 28807980
1971 NB1, a new neutrophil-specific antigen involved in the pathogenesis of neonatal neutropenia. The Journal of clinical investigation 130 5552408
2002 Discrimination of polycythemias and thrombocytoses by novel, simple, accurate clonality assays and comparison with PRV-1 expression and BFU-E response to erythropoietin. Blood 129 12515724
2021 CD177, a specific marker of neutrophil activation, is associated with coronavirus disease 2019 severity and death. iScience 127 34127958
2007 NB1 mediates surface expression of the ANCA antigen proteinase 3 on human neutrophils. Blood 103 17244676
2006 Proteinase 3 and CD177 are expressed on the plasma membrane of the same subset of neutrophils. Journal of leukocyte biology 102 17077162
1990 Biochemical characterization of the neutrophil-specific antigen NB1. Blood 91 2153425
2005 The Jak2V617F mutation, PRV-1 overexpression, and EEC formation define a similar cohort of MPD patients. Blood 87 15985544
2021 CD177 modulates the function and homeostasis of tumor-infiltrating regulatory T cells. Nature communications 83 34599187
2010 Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation. The Journal of biological chemistry 83 21193407
2020 Generation of pancreatic β cells from CD177+ anterior definitive endoderm. Nature biotechnology 80 32341565
2013 Higgs amplitude mode in the BCS superconductors Nb1-xTi(x)N induced by terahertz pulse excitation. Physical review letters 79 23952432
2001 Molecular basis of the neutrophil glycoprotein NB1 (CD177) involved in the pathogenesis of immune neutropenias and transfusion reactions. European journal of immunology 79 11465086
1994 Early tagma-specific commitment of Drosophila CNS progenitor NB1-1. Development (Cambridge, England) 75 7956832
2003 Quantification of PRV-1 mRNA distinguishes polycythemia vera from secondary erythrocytosis. Blood 73 12893745
2005 Concomitant neutrophil JAK2 mutation screening and PRV-1 expression analysis in myeloproliferative disorders and secondary polycythaemia. British journal of haematology 70 16197445
2004 CD177: A member of the Ly-6 gene superfamily involved with neutrophil proliferation and polycythemia vera. Journal of translational medicine 70 15050027
2009 Coexpression of CD177 and membrane proteinase 3 on neutrophils in antineutrophil cytoplasmic autoantibody-associated systemic vasculitis: anti-proteinase 3-mediated neutrophil activation is independent of the role of CD177-expressing neutrophils. Arthritis and rheumatism 69 19404956
2004 Neutrophil CD177 (NB1 gp, HNA-2a) expression is increased in severe bacterial infections and polycythaemia vera. British journal of haematology 68 15238147
2007 Neutrophil-specific antigen HNA-2a, NB1 glycoprotein, and CD177. Current opinion in hematology 67 17898576
2010 Neutrophil transmigration mediated by the neutrophil-specific antigen CD177 is influenced by the endothelial S536N dimorphism of platelet endothelial cell adhesion molecule-1. Journal of immunology (Baltimore, Md. : 1950) 64 20194726
2012 Proteinase 3 contributes to transendothelial migration of NB1-positive neutrophils. Journal of immunology (Baltimore, Md. : 1950) 63 22266279
2016 Identification of CD177 as the most dysregulated parameter in a microarray study of purified neutrophils from septic shock patients. Immunology letters 61 27568821
2000 Variations in the expression of granulocyte antigen NB1. Transfusion 60 10864984
2004 PRV-1 mRNA expression discriminates two types of essential thrombocythemia. Annals of hematology 55 15034760
2008 A hydrophobic patch on proteinase 3, the target of autoantibodies in Wegener granulomatosis, mediates membrane binding via NB1 receptors. The Journal of biological chemistry 53 18854317
2013 IL-17-producing peripheral blood CD177+ neutrophils increase in allergic asthmatic subjects. Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology 52 23822853
2022 CD177+ cells produce neutrophil extracellular traps that promote biliary atresia. Journal of hepatology 51 35803543
2002 Modulation of neuritogenesis by ganglioside-specific sialidase (Neu 3) in human neuroblastoma NB-1 cells. Neurochemical research 51 12374221
2010 Elevated neutrophil membrane expression of proteinase 3 is dependent upon CD177 expression. Clinical and experimental immunology 48 20491791
2015 Genetic mapping of the nulliplex-branch gene (gb_nb1) in cotton using next-generation sequencing. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 47 25575840
2002 Biochemical characterization of PRV-1, a novel hematopoietic cell surface receptor, which is overexpressed in polycythemia rubra vera. Blood 47 12239154
2010 Comparison of human islet isolation outcomes using a new mammalian tissue-free enzyme versus collagenase NB-1. Transplantation 45 20463640
2014 Differential expression of granulopoiesis related genes in neutrophil subsets distinguished by membrane expression of CD177. PloS one 43 24926686
2003 Lack of NB1 GP (CD177/HNA-2a) gene transcription in NB1 GP- neutrophils from NB1 GP-expressing individuals and association of low expression with NB1 gene polymorphisms. Blood 43 12623849
2014 Characterization of a novel mouse model with genetic deletion of CD177. Protein & cell 42 25359465
2002 Molecular basis of NB1 (HNA-2a, CD177) deficiency. Blood 42 12010833
2017 Evaluation of 11C-Me-NB1 as a Potential PET Radioligand for Measuring GluN2B-Containing NMDA Receptors, Drug Occupancy, and Receptor Cross Talk. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 41 29191857
2012 Deregulation of apoptosis-related genes is associated with PRV1 overexpression and JAK2 V617F allele burden in Essential Thrombocythemia and Myelofibrosis. Journal of hematology & oncology 41 22300941
2017 Gene silencing and a novel monoallelic expression pattern in distinct CD177 neutrophil subsets. The Journal of experimental medicine 38 28559244
2018 CD177-mediated nanoparticle targeting of human and mouse neutrophils. PloS one 37 29990379
2016 Heterogeneity of Human Neutrophil CD177 Expression Results from CD177P1 Pseudogene Conversion. PLoS genetics 33 27227454
2020 The neutrophil subset defined by CD177 expression is preferentially recruited to gingival crevicular fluid in periodontitis. Journal of leukocyte biology 32 32531826
2009 Age- and division-of-labour-dependent differential expression of a novel non-coding RNA, Nb-1, in the brain of worker honeybees, Apis mellifera L. Insect molecular biology 32 19817910
2009 Large-scale comparison of Liberase HI and collagenase NB1 utilized for human islet isolation. Cell transplantation 32 19818208
2004 Clinical significance of neutrophil CD177 mRNA expression in Ph-negative chronic myeloproliferative disorders. British journal of haematology 32 15327515
1996 Analysis of the expression of NB1 antigen using two monoclonal antibodies. Transfusion 32 8614969
2009 Molecular studies reveal that A134T, G156A and G1333A SNPs in the CD177 gene are associated with atypical expression of human neutrophil antigen-2. Vox sanguinis 31 19695014
2003 Neutrophil PRV-1 expression across the chronic myeloproliferative disorders and in secondary or spurious polycythemia. Blood 31 14701686
1991 Neutrophil-specific antigen NB1 is anchored via a glycosyl-phosphatidylinositol linkage. Journal of leukocyte biology 31 1825110
2015 Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX. Food research international (Ottawa, Ont.) 28 28411984
2003 Expression of human neutrophil antigen-2a (NB1) is increased in pregnancy. Transfusion 28 12675722
2019 Preclinical Evaluation of Benzazepine-Based PET Radioligands (R)- and (S)-11C-Me-NB1 Reveals Distinct Enantiomeric Binding Patterns and a Tightrope Walk Between GluN2B- and σ1-Receptor-Targeted PET Imaging. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 26 30683765
2006 The gene overexpressed in polycythemia rubra vera, PRV-1, and the gene encoding a neutrophil alloantigen, NB1, are alleles of a single gene, CD177, in chromosome band 19q13.31. Transfusion 26 16533288
2021 Triggering Receptor Expressed on Myeloid Cells-1 Agonist Regulates Intestinal Inflammation via Cd177+ Neutrophils. Frontiers in immunology 25 33767714
2019 Increase expression of CD177 in Kawasaki disease. Pediatric rheumatology online journal 25 30943984
2002 The use of bioinformatics to identify the genomic structure of the gene that encodes neutrophil antigen NB1, CD177. Clinical immunology (Orlando, Fla.) 25 11846455
1998 The expression of the NB1 antigen on myeloid precursors and neutrophils from children and umbilical cords. Transfusion medicine (Oxford, England) 25 9675788
2020 Consequences of Piscine orthoreovirus genotype 1 (PRV-1) infections in Chinook salmon (Oncorhynchus tshawytscha), coho salmon (O. kisutch) and rainbow trout (O. mykiss). Journal of fish diseases 24 32476167
2020 Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome. Pathogens (Basel, Switzerland) 24 33053677
1987 Biological properties of a tumour cell line (NB1-G) derived from human neuroblastoma. British journal of cancer 24 2437946
2021 First-in-Humans Brain PET Imaging of the GluN2B-Containing N-methyl-d-aspartate Receptor with (R)-11C-Me-NB1. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 22 34620732
2001 Immune neutropenia associated with anti-human neutrophil antigen-2a (NB1) antibodies following unrelated donor stem cell transplantation for chronic myeloid leukaemia: perpetuation by granulocyte colony-stimulating factor. British journal of haematology 22 11380420
1993 Quinine-dependent antibodies to neutrophils react with a 60-Kd glycoprotein on which neutrophil-specific antigen NB1 is located and an 85-Kd glycosyl-phosphatidylinositol-linked N-glycosylated plasma membrane glycoprotein. Blood 22 8387837
2021 Inflammatory signature in acute-on-chronic liver failure includes increased expression of granulocyte genes ELANE, MPO and CD177. Scientific reports 21 34552111
2020 Preclinical Development of 18F-OF-NB1 for Imaging GluN2B-Containing N-Methyl-d-Aspartate Receptors and Its Utility as a Biomarker for Amyotrophic Lateral Sclerosis. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 21 32737247
2003 Increased risk for vascular complications in PRV-1 positive patients with essential thrombocythaemia. British journal of haematology 21 14617016
2023 Dysregulation of CD177+ neutrophils on intraepithelial lymphocytes exacerbates gut inflammation via decreasing microbiota-derived DMF. Gut microbes 19 36729914
2004 Significance of human neutrophil antigen-2a (NB1) expression and neutrophil number in pregnancy. Transfusion 19 15043575
2004 Serva collagenase NB1: a new enzyme preparation for human islet isolation. Transplantation proceedings 19 15194398
2003 The presence of a significant association between elevated PRV-1 mRNA expression and low plasma erythropoietin concentration in essential thrombocythaemia. European journal of haematology 19 12756017
2021 PRV-1 Infected Macrophages in Melanized Focal Changes in White Muscle of Atlantic Salmon (Salmo salar) Correlates With a Pro-Inflammatory Environment. Frontiers in immunology 18 33995395
2016 A sequence-specific polymerase chain reaction method for HNA-2 genotyping: homozygous c.843A>T mutation predicts the absence of CD177. Transfusion 18 27385476
2013 CD177 expression on neutrophils: in search of a clonal assay for myeloid neoplasia by flow cytometry. American journal of clinical pathology 18 24124144
2012 Comparative evaluation of the role of the adhesion molecule CD177 in neutrophil interactions with platelets and endothelium. European journal of haematology 18 22690867
2007 Phenoxazine derivatives 2-amino-4,4alpha-dihydro-4alpha-phenoxazine-3-one and 2-aminophenoxazine-3-one-induced apoptosis through a caspase-independent mechanism in human neuroblastoma cell line NB-1 cells. Biological & pharmaceutical bulletin 18 17268075
2004 Platelet-rich plasma serotonin levels in chronic myeloproliferative disorders: evaluation of diagnostic use and comparison with the neutrophil PRV-1 assay. British journal of haematology 18 15384975
2007 Biomarker analysis in polycythemia vera under interferon-alpha treatment: clonality, EEC, PRV-1, and JAK2 V617F. Annals of hematology 17 17256145
2020 CD177 Enhances the Detection of Myelodysplastic Syndrome by Flow Cytometry. American journal of clinical pathology 16 32011681
2020 Cancer cell-intrinsic function of CD177 in attenuating β-catenin signaling. Oncogene 16 32042113
2004 PRV-1, erythroid colonies and platelet Mpl are unrelated to thrombosis in essential thrombocythaemia. British journal of haematology 16 15461629
2024 Dual role of CD177 + neutrophils in inflammatory bowel disease: a review. Journal of translational medicine 15 39223577
2019 The nonconservative CD177 single-nucleotide polymorphism c.1291G>A is a genetic determinant for human neutrophil antigen-2 atypical/low expression and deficiency. Transfusion 15 30828823
2018 Interaction between CD177 and platelet endothelial cell adhesion molecule-1 downregulates membrane-bound proteinase-3 (PR3) expression on neutrophils and attenuates neutrophil activation induced by PR3-ANCA. Arthritis research & therapy 15 30236159
1993 Polyclonal antibodies against the NB1-bearing 58- to 64-kDa glycoprotein of human neutrophils do not identify an NB2-bearing molecule. Transfusion 15 8488543
2025 Targeting mitochondrial complex I of CD177+ neutrophils alleviates lung ischemia-reperfusion injury. Cell reports. Medicine 14 40398393
2020 Dissemination of Piscine orthoreovirus-1 (PRV-1) in Atlantic Salmon (Salmo salar) during the Early and Regenerating Phases of Infection. Pathogens (Basel, Switzerland) 14 32093243
2019 Kidney injury enhances renal G-CSF expression and modulates granulopoiesis and human neutrophil CD177 in vivo. Clinical and experimental immunology 14 31509227
2005 Inhibition of thrombin-induced vascular endothelial growth factor production in human neuroblastoma (NB-1) cells by argatroban. Pathophysiology of haemostasis and thrombosis 14 16293985
1994 Neutrophil-specific antigen NB1 inhibits neutrophil-endothelial cell interactions. The Journal of laboratory and clinical medicine 14 8301201
2024 Nb1.60Ti0.32W0.08O5-δ as negative electrode active material for durable and fast-charging all-solid-state Li-ion batteries. Nature communications 13 39396046
2022 Evaluation of (rac)-, (R)-, and (S)-18F-OF-NB1 for Imaging GluN2B Subunit-Containing N-Methyl-d-Aspartate Receptors in Nonhuman Primates. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 13 35710735
2021 Piscine Orthoreovirus (PRV)-3, but Not PRV-2, Cross-Protects against PRV-1 and Heart and Skeletal Muscle Inflammation in Atlantic Salmon. Vaccines 13 33800725
2021 The neutrophil protein CD177 is a novel PDPN receptor that regulates human cancer-associated fibroblast physiology. PloS one 13 34879110
2015 MnSOD downregulation induced by extremely low 0.1 mGy single and fractionated X-rays and microgravity treatment in human neuroblastoma cell line, NB-1. Journal of clinical biochemistry and nutrition 13 26388666
1997 Synthesis and purification of NB1-palmitoyl insulin. Journal of pharmaceutical sciences 13 9383737
1993 Analysis of the expression of neutrophil-specific antigen NB1: characterization of neutrophils that react with but are not agglutinated by anti-NB1. Transfusion 13 8342232

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