Affinage

CD177

CD177 antigen · UniProt Q8N6Q3

Length
437 aa
Mass
46.4 kDa
Annotated
2026-04-28
100 papers in source corpus 24 papers cited in narrative 24 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

CD177 is a GPI-anchored neutrophil surface glycoprotein of the uPAR/Ly6/CD59 superfamily that orchestrates neutrophil transendothelial migration, activation, and survival through engagement of endothelial PECAM-1 and presentation of proteinase 3 (PR3) at the cell surface. CD177 binds PECAM-1 domain 6 in a cation-dependent manner to facilitate neutrophil transmigration, simultaneously signaling through a β2 integrin (Mac-1/CD11b) complex that activates Src and ERK pathways (PMID:17580308, PMID:21193407, PMID:28807980). CD177 serves as the obligate membrane receptor for PR3, enabling PR3-ANCA-mediated neutrophil degranulation and respiratory burst, while PECAM-1 engagement downregulates membrane PR3 to attenuate this activation (PMID:17244676, PMID:30236159). Subset-restricted bimodal expression on neutrophils is governed by monoallelic epigenetic silencing through CpG methylation and AP-1 (c-Jun/c-Fos) transcription factor binding, and CD177 deficiency in mice causes increased neutrophil death, exacerbated colitis with impaired barrier function, and altered NET-dependent tissue injury (PMID:28559244, PMID:25359465, PMID:28468761, PMID:35803543).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1990 High

    Establishing the molecular identity of CD177 resolved that the NB1 neutrophil alloantigen is a 58–64 kDa surface glycoprotein stored in secondary granules, with a conformational (non-carbohydrate) epitope.

    Evidence SDS-PAGE immunoblotting, immunoprecipitation with 125I-labeled neutrophils, differential centrifugation fractionation of granule subsets

    PMID:2153425

    Open questions at the time
    • Primary structure unknown
    • Membrane anchoring mechanism not yet determined
    • Function of NB1 entirely unknown
  2. 1991 High

    Demonstrating that NB1 is GPI-anchored established its mode of membrane attachment and explained its absence in PNH patients lacking GPI-linked proteins.

    Evidence PI-PLC enzymatic release of 125I-surface-labeled NB1 from neutrophils, endoglycosidase-F deglycosylation

    PMID:1825110

    Open questions at the time
    • Gene identity and sequence unknown
    • No known ligand or function
  3. 1994 Medium

    Functional studies first indicated that NB1 modulates neutrophil-endothelial interactions, with NB1-positive neutrophils showing reduced basal adhesion to endothelium but enhanced chemotaxis.

    Evidence HUVEC adhesion assay and Fab fragment blocking of NB1, chemotaxis through nitrocellulose

    PMID:8301201

    Open questions at the time
    • Endothelial ligand for NB1 unknown
    • Mechanism of adhesion modulation unclear
    • Single lab observation
  4. 2001 High

    Molecular cloning of CD177 revealed it as a 437-amino-acid member of the uPAR/Ly6/CD59 superfamily with two cysteine-rich domains and three N-glycosylation sites, providing the sequence foundation for all subsequent mechanistic work.

    Evidence MALDI-TOF MS, N-terminal sequencing, RACE PCR, cDNA cloning and expression in COS-7 cells

    PMID:11465086

    Open questions at the time
    • Physiological ligand and function still unknown
    • Basis for bimodal expression pattern not explained
  5. 2003 High

    Demonstrating that CD177-negative neutrophils lack NB1 mRNA and that cDNA polymorphisms correlate with small CD177+ subsets established that bimodal expression is transcriptionally regulated with a genetic component.

    Evidence Single-cell FACS sorting followed by RT-PCR, cDNA polymorphism analysis, G-CSF stimulation

    PMID:12623849

    Open questions at the time
    • Epigenetic vs. purely genetic mechanism undetermined
    • Promoter regulation unexplored
  6. 2007 High

    Two simultaneous discoveries identified CD177's two key molecular partners: PECAM-1 as its heterophilic endothelial ligand mediating transendothelial migration, and PR3 as its surface-presented cargo, linking CD177 to both neutrophil trafficking and ANCA-mediated activation.

    Evidence Co-IP, SPR, antibody blocking of transmigration for PECAM-1 interaction; co-IP, confocal co-localization, transfection binding, and GPI removal for PR3 interaction

    PMID:17244676 PMID:17580308

    Open questions at the time
    • Transmembrane signaling mechanism unknown (CD177 is GPI-anchored)
    • Structural basis of CD177-PECAM-1 and CD177-PR3 interfaces unresolved
    • In vivo relevance of each interaction not tested
  7. 2008 High

    Mapping the PR3 binding interface to a species-specific hydrophobic cluster (F166/W218/G219/L223) defined the structural determinants for CD177-PR3 interaction and showed that surface-bound PR3 retains enzymatic activity.

    Evidence Human-gibbon PR3 chimeric proteins and binding assays to NB1-expressing cells

    PMID:18854317

    Open questions at the time
    • Reciprocal mapping of the CD177 binding surface for PR3 not done
    • No crystal structure of the complex
  8. 2010 High

    Identification of Mac-1 (CD11b/CD18) as the transmembrane signaling adaptor for GPI-anchored CD177 resolved how CD177 transduces signals, and showing allele-specific modulation of PECAM-1 ITIM phosphorylation and β-catenin phosphorylation explained how CD177 regulates endothelial junctional integrity during transmigration.

    Evidence MS/MS proteomics of NB1 signaling complexes, SPR for direct interaction, lipid raft co-localization, CD11b blocking of PR3-ANCA responses; transendothelial migration with PECAM-1 allelic variants and phosphorylation assays

    PMID:20194726 PMID:21193407

    Open questions at the time
    • Structural basis of CD177-Mac-1 interaction unknown
    • In vivo validation of allele-specific migration not performed
  9. 2012 High

    Demonstrating that PR3 enzymatic activity selectively enhances transmigration of CD177-positive neutrophils, and that CD177-PECAM-1 interaction does not mediate platelet-neutrophil conjugation, refined the specificity of CD177's functional roles to endothelial transmigration.

    Evidence Transendothelial migration with selective serine protease inhibitors under static and flow conditions; platelet-neutrophil conjugate assays

    PMID:22266279 PMID:22690867

    Open questions at the time
    • PR3 substrates at the endothelial junction not identified
    • Relative contributions of CD177-PECAM-1 binding versus PR3 proteolysis to transmigration not quantified
  10. 2017 High

    Three key advances in 2017 completed the signaling model: CD177 ligation enhances β2 integrin interaction via FLIM, activates Src/ERK to paradoxically impair migration through integrin internalization defects; epigenetic control was shown to involve monoallelic CpG methylation and AP-1 binding; and CD177+ neutrophils were demonstrated to be anti-inflammatory in IBD with IL-22 production and barrier-protective functions.

    Evidence FLIM, phosphorylation/signaling inhibitor assays; ChIP, bisulfite sequencing, reporter assays; CD177−/− mouse DSS colitis model with cytokine profiling and RNA-seq

    PMID:28468761 PMID:28559244 PMID:28807980

    Open questions at the time
    • How Src/ERK activation simultaneously promotes transmigration yet impairs chemokine-directed migration is not fully reconciled
    • Whether monoallelic expression is stochastic or deterministic during granulopoiesis is unclear
    • Mechanism linking CD177 to IL-22 production not defined
  11. 2018 Medium

    Establishing that PECAM-1 engagement of CD177 downregulates membrane PR3 and attenuates PR3-ANCA-induced activation revealed a negative feedback loop that may limit ANCA vasculitis pathology in CD177-positive neutrophils.

    Evidence Dose-dependent PR3 downregulation by recombinant PECAM-1, degranulation and oxidative burst assays with MACS-sorted CD177-negative controls

    PMID:30236159

    Open questions at the time
    • In vivo relevance in ANCA vasculitis not tested
    • Mechanism of PECAM-1-induced PR3 internalization not defined
    • Single lab observation
  12. 2020 Medium

    Discovery that CD177 marks anterior definitive endoderm fated toward pancreas and that CD177 deficiency increases β-catenin/Wnt signaling in mammary epithelium extended CD177 function beyond neutrophils to developmental and tumor-suppressive contexts.

    Evidence hPSC differentiation with FACS sorting and scRNA-seq; CD177 KO/KD with Wnt signaling reporters in mammary cells

    PMID:32042113 PMID:32341565

    Open questions at the time
    • Mechanism by which CD177 inhibits Wnt/β-catenin signaling is unknown
    • Whether CD177's developmental role is cell-autonomous or paracrine is unclear
    • Single lab for each observation
  13. 2021 Medium

    CD177 was found to function on tumor-infiltrating Tregs to sustain immunosuppression and was identified as a novel receptor for podoplanin on cancer-associated fibroblasts, broadening CD177's role to immune regulation in the tumor microenvironment.

    Evidence Treg-specific Cd177 conditional KO mice with tumor growth assays and in vitro suppression; high-throughput receptor-ligand screening with phosphoproteomics for PDPN interaction

    PMID:34599187 PMID:34879110

    Open questions at the time
    • Signaling downstream of CD177 in Tregs is uncharacterized
    • CD177-PDPN interaction awaits independent replication
    • Whether CD177 on Tregs engages PECAM-1 or PDPN in vivo is unknown
  14. 2022 High

    Demonstrating that CD177+ neutrophils produce NETs that cause biliary epithelial apoptosis in biliary atresia, with amelioration in Cd177−/− mice, established CD177 as a pathogenic driver through NET-mediated tissue injury.

    Evidence Cd177−/− mouse BA model, Smart-Seq RNA-seq, co-culture apoptosis assay, NET inhibitor studies

    PMID:35803543

    Open questions at the time
    • Whether CD177 directly promotes NET formation or acts indirectly through survival/ROS is unclear
    • Human genetic validation in biliary atresia patients not provided

Open questions

Synthesis pass · forward-looking unresolved questions
  • No atomic-resolution structure of CD177 or its complexes with PECAM-1, PR3, Mac-1, or podoplanin exists, and the mechanism by which this GPI-anchored protein modulates intracellular Wnt/β-catenin signaling and Treg suppressive function remains undefined.
  • No crystal or cryo-EM structure of CD177 or any CD177-ligand complex
  • Mechanism of CD177-mediated Wnt pathway inhibition unknown
  • How CD177 regulates Treg suppressive function at the molecular level is unexplored
  • Whether neutrophil and non-neutrophil functions share common signaling mechanisms is untested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098631 cell adhesion mediator activity 4 GO:0098772 molecular function regulator activity 3
Localization
GO:0005886 plasma membrane 5 GO:0031410 cytoplasmic vesicle 2 GO:0005576 extracellular region 1
Pathway
R-HSA-168256 Immune System 6 R-HSA-162582 Signal Transduction 4 R-HSA-1500931 Cell-Cell communication 3
Partners
FOSITGAMJUNPDPNPECAM1PRTN3
Complex memberships
CD177–Mac-1 (CD11b/CD18) lipid raft signaling complexCD177–PR3 surface complex

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2007 CD177 (NB1) was identified as a heterophilic binding partner of PECAM-1 (CD31) on endothelial cells. Flow cytometry, immunoprecipitation, and surface plasmon resonance confirmed a cation-dependent, specific interaction between CD177 and PECAM-1 domain 6. Monoclonal antibodies against CD177 and PECAM-1 domain 6 inhibited adhesion of CD177-expressing U937 cells to immobilized PECAM-1 and blocked transendothelial migration of human neutrophils. Flow cytometry, immunoprecipitation, surface plasmon resonance, antibody blocking of transendothelial migration The Journal of biological chemistry High 17580308
2007 NB1 (CD177) mediates surface expression of proteinase 3 (PR3) on human neutrophils. PR3 and NB1 co-immunoprecipitated and co-localized on the neutrophil plasma membrane. Transfection of NB1 into HEK293 and HL60 cells resulted in specific PR3 surface binding. Enzymatic removal of GPI anchors decreased mPR3 expression, and mPR3 was absent in a paroxysmal nocturnal hemoglobinuria patient lacking GPI-anchored proteins. Co-immunoprecipitation, confocal microscopy, flow cytometry, GPI anchor removal, cell transfection Blood High 17244676
2010 Mac-1 (CD11b/CD18) was identified as a transmembrane adaptor for NB1 (CD177) signaling. MS/MS analysis of NB1 signaling complexes precipitated from plasma membranes identified Mac-1 as the key component. Direct protein-protein interaction between NB1 and CD11b/CD11a was confirmed by surface plasmon resonance, but only Mac-1-transfected cells adhered to immobilized NB1. NB1, PR3, and Mac-1 co-localized in lipid rafts. Blocking CD11b inhibited PR3-ANCA-induced neutrophil degranulation and superoxide production. MS/MS proteomics, immunoprecipitation, surface plasmon resonance, transfection adhesion assay, confocal microscopy, lipid raft fractionation, blocking antibodies The Journal of biological chemistry High 21193407
2017 CD177 modulates neutrophil migration through β2 integrin-dependent signaling. CD177 ligation enhanced its interaction with β2 integrins (revealed by fluorescence lifetime imaging microscopy), leading to integrin-mediated phosphorylation of Src and ERK. This caused increased surface β2 integrin expression and affinity, impaired integrin attachment internalization, and ERK-mediated attenuation of chemokine signaling, collectively impairing neutrophil migration. Fluorescence lifetime imaging microscopy (FLIM), phosphorylation assays, transwell migration, flow cytometry, signaling inhibitors Blood High 28807980
2008 A hydrophobic cluster unique to human PR3 (residues F166, W218, G219, L223) mediates its binding to the NB1 receptor on the neutrophil surface. Gibbon PR3, which lacks these residues, did not bind human NB1. A human-gibbon hybrid PR3 lacking the C-terminal human residues also failed to bind NB1. NB1-bound PR3 remained enzymatically active and was cleared from the surface by alpha-1-protease inhibitor. Cloning and expression of gibbon PR3 ortholog, human-gibbon hybrid protein construction, NB1 binding assay, functional enzymatic assay The Journal of biological chemistry High 18854317
2010 CD177-positive neutrophils migrate significantly faster through endothelial cells expressing the LSR allelic variant of PECAM-1 compared to VNG variant. Engagement of PECAM-1 by rCD177-Fc suppressed ITIM tyrosine phosphorylation and increased β-catenin phosphorylation in an allele-specific manner, suggesting that CD177/PECAM-1 heterophilic interaction modulates endothelial junctional integrity to facilitate neutrophil transmigration. In vitro transendothelial migration assay, PECAM-1 ITIM phosphorylation measurement, β-catenin phosphorylation, recombinant CD177-Fc protein Journal of immunology High 20194726
2012 Proteinase 3 (PR3) activity contributes to transendothelial migration selectively in NB1 (CD177)-positive neutrophils. PR3 expression and activity were significantly increased on NB1-positive neutrophils following transmigration. Using selective serine protease inhibitors, PR3 activity was shown to facilitate transmigration of NB1-positive neutrophils under both static and flow conditions, while NB1-negative neutrophils showed no increase in PR3. In vitro transendothelial migration under static and flow conditions, selective serine protease inhibitors, flow cytometry Journal of immunology High 22266279
2006 CD177 and membrane PR3 are expressed on the same neutrophil subset; their surface expression increases and decreases in parallel during cell stimulation or spontaneous apoptosis. Rapid internalization and recirculation of both mPR3 and CD177 occur together (all mPR3 replaced within 30 min), indicating they share the same intracellular storage compartment: secondary granules and secretory vesicles. Flow cytometry, cell stimulation assays, apoptosis assays, subcellular fractionation inference Journal of leukocyte biology Medium 17077162
2001 CD177 (NB1) was biochemically characterized as a 437-amino-acid GPI-anchored glycoprotein with two cysteine-rich domains homologous to the uPAR/Ly6/CD59/snake toxin superfamily, three N-linked glycosylation sites, and a GPI attachment site. COS-7 cells transfected with NB1 cDNA expressed immunoreactive NB1 glycoprotein, confirming the protein-coding sequence. MALDI-TOF mass spectrometry, N-terminal sequencing, RACE PCR, cDNA cloning, COS-7 transfection European journal of immunology High 11465086
2002 PRV-1 (CD177) protein is N-glycosylated and GPI-anchored, confirmed biochemically. Multiple PRV-1 transcripts arise from alternative polyadenylation and encode the same protein. Soluble CD177 is shed from the cell surface and can be detected in cell supernatants. Stably transfected cells expressing PRV-1 are recognized by anti-NB1/CD177 antibodies, confirming PRV-1 and NB1 are products of the same gene. Northern blot, biochemical deglycosylation, PI-PLC treatment, flow cytometry, FACS analysis of transfected cells Blood High 12239154
1991 The NB1 antigen (CD177) is anchored to the neutrophil plasma membrane via a glycosyl-phosphatidylinositol (GPI) linkage. Treatment with PI-PLC specifically released the 58–64 kDa NB1-bearing protein from the cell surface. Removal of N-linked carbohydrates with endoglycosidase-F reduced the apparent molecular weight to ~45 kDa. PI-PLC treatment, 125I surface labeling, non-denaturing gel electrophoresis, endoglycosidase-F treatment Journal of leukocyte biology High 1825110
1990 The NB1 antigen resides on a 58–64 kDa surface glycoprotein also present in secondary granules but not primary granules of neutrophils. The NB1 epitope is not a carbohydrate but likely resides in the tertiary structure of the protein backbone, as periodate treatment had no effect but reduction abolished antibody recognition. SDS-PAGE immunoblotting, immunoprecipitation with 125I-labeled neutrophils, differential centrifugation fractionation, periodate treatment, reduction Blood High 2153425
2017 CD177 expression in neutrophils is controlled by epigenetic mechanisms including a novel monoallelic expression pattern. In bimodal individuals, CD177pos neutrophils transcribe either the maternal or paternal CD177 allele exclusively. ChIP and reporter assays showed that CpG methylation of the CD177 promoter reduces transcription, while demethylation causes biallelic expression. c-Jun and c-Fos bound the CD177 promoter in CD177pos neutrophils (which have a euchromatic, unmethylated promoter) but not CD177neg neutrophils. ChIP, reporter assays (HeLa cells), haplotype analysis, bisulfite sequencing, transcription factor transfection The Journal of experimental medicine High 28559244
2003 CD177-negative neutrophils within bimodal individuals lack NB1 mRNA, and this absence persists even after G-CSF administration. Three CD177 cDNA polymorphisms were significantly associated with a small CD177-expressing neutrophil subpopulation, indicating a genetic basis for subset-restricted expression. Single-cell picking, FACS sorting, RT-PCR, cDNA sequencing, G-CSF stimulation Blood High 12623849
2014 CD177 knockout mice show decreased neutrophil counts in peripheral blood and reduced neutrophil accumulation in skin infected with Staphylococcus aureus. CD177 deletion had no significant impact on CXCL1/KC- or fMLP-induced migration in vitro but led to significant neutrophil cell death, indicating CD177 supports neutrophil survival rather than chemotaxis per se. Genetic knockout mouse model, skin infection model with S. aureus, in vitro migration assays, flow cytometry Protein & cell High 25359465
2021 CD177 expressed on tumor-infiltrating regulatory T cells (TI Tregs) modulates their suppressive activity. Blocking CD177 reduced the suppressive activity of Tregs in vitro, while Treg-specific deletion of Cd177 in mice led to decreased tumor growth and reduced TI Treg frequency, establishing a functional role for CD177 in TI Treg-mediated immunosuppression. scRNA-seq, in vitro Treg suppression assay with CD177 blocking, conditional Treg-specific Cd177 knockout mice, tumor growth assay Nature communications High 34599187
2020 CD177 marks anterior definitive endoderm (ADE) specified toward pancreatic fate. CD177+ ADE expresses and synthesizes the secreted WNT/NODAL/BMP antagonist CERBERUS1 and shows inverse canonical vs. noncanonical WNT signaling compared to CD275+ (hepatic) ADE. Isolated CD177+ ADE differentiates more homogeneously into pancreatic progenitors and more functional glucose-responsive β-like cells. Flow cytometry sorting, scRNA-seq, differentiation assays from human pluripotent stem cells, glucose stimulation assay Nature biotechnology Medium 32341565
2020 CD177 deficiency in cancer cells is associated with increased β-catenin/Wnt signaling. Loss of CD177 leads to hyperproliferative mammary epithelium. CD177 was identified as a novel regulator of mammary epithelial proliferation acting via modulation of the Wnt/β-catenin signaling pathway. CD177 loss-of-function (KO/KD), proliferation assays, β-catenin signaling reporters and immunoblot Oncogene Medium 32042113
2021 CD177 was identified as a novel receptor for podoplanin (PDPN) on cancer-associated fibroblasts. High-throughput interactome screening identified the CD177-PDPN interaction. CD177 acts as a functional antagonist of PDPN, recapitulating the phenotype of PDPN-deficient CAFs including effects on cell signaling, growth, and actomyosin contractility. High-throughput receptor-ligand screening, quantitative phosphoproteomics, cellular functional assays PloS one Medium 34879110
2018 PECAM-1 interaction with CD177 downregulates membrane PR3 (mPR3) expression on neutrophils in a dose-dependent manner and attenuates PR3-ANCA-induced neutrophil degranulation and respiratory burst. This effect was CD177-dependent: in CD177-negative neutrophils, PECAM-1 did not significantly change degranulation induced by PR3-ANCA. ELISA, flow cytometry, DHR oxidative burst assay, MACS sorting of CD177-negative neutrophils, degranulation assay Arthritis research & therapy Medium 30236159
2017 CD177+ neutrophils in IBD produce lower levels of proinflammatory cytokines (IFN-γ, IL-6, IL-17A) but higher levels of IL-22 and TGF-β, and exhibit increased bactericidal activities (ROS, antimicrobial peptides, NETs) compared to CD177- neutrophils. CD177-/- mice developed more severe DSS colitis with compromised intestinal barrier, impaired antibacterial immunity, and decreased IL-22 production. Flow cytometry, RNA sequencing, CD177-/- mouse DSS colitis model, cytokine quantification, bactericidal assays Gut High 28468761
2022 CD177+ cells produce neutrophil extracellular traps (NETs) that promote biliary atresia (BA) pathogenesis. Cd177-/- mice exhibited delayed BA onset and reduced morbidity/mortality. CD177+ cells from BA mice had high mitochondrial content, elevated ROS, and increased NET formation; NETs induced apoptosis of biliary epithelial cells in co-culture. Cd177-/- mouse model, Smart-Seq RNA-seq, co-culture apoptosis assay, ROS measurement, NET inhibitor studies Journal of hepatology High 35803543
2012 CD177 interaction with PECAM-1 supports preferential transendothelial migration of CD177-positive neutrophils, but CD177/PECAM-1 interaction does not mediate platelet-neutrophil interactions; CD177-positive and CD177-negative neutrophils formed platelet conjugates equally under static and flow conditions. Whole blood platelet-neutrophil conjugate assay, flow chamber adhesion assay, HUVEC migration assay European journal of haematology Medium 22690867
1994 NB1 antigen (CD177) inhibits neutrophil-endothelial cell interactions. Unstimulated NB1-positive neutrophils adhered less to HUVEC monolayers than NB1-negative neutrophils. Incubation with anti-NB1 Fab fragments increased CD177+ neutrophil adherence to HUVEC, suggesting NB1 normally suppresses adhesion to endothelium. NB1-positive neutrophils showed greater chemotaxis to FMLP through nitrocellulose. HUVEC adhesion assay, Fab fragment blocking, chemotaxis assay The Journal of laboratory and clinical medicine Medium 8301201

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2017 CD177+ neutrophils as functionally activated neutrophils negatively regulate IBD. Gut 217 28468761
2007 The neutrophil-specific antigen CD177 is a counter-receptor for platelet endothelial cell adhesion molecule-1 (CD31). The Journal of biological chemistry 192 17580308
2000 Cloning of PRV-1, a novel member of the uPAR receptor superfamily, which is overexpressed in polycythemia rubra vera. Blood 171 10753836
2017 CD177 modulates human neutrophil migration through activation-mediated integrin and chemoreceptor regulation. Blood 146 28807980
2002 Discrimination of polycythemias and thrombocytoses by novel, simple, accurate clonality assays and comparison with PRV-1 expression and BFU-E response to erythropoietin. Blood 129 12515724
1971 NB1, a new neutrophil-specific antigen involved in the pathogenesis of neonatal neutropenia. The Journal of clinical investigation 129 5552408
2021 CD177, a specific marker of neutrophil activation, is associated with coronavirus disease 2019 severity and death. iScience 121 34127958
2006 Proteinase 3 and CD177 are expressed on the plasma membrane of the same subset of neutrophils. Journal of leukocyte biology 102 17077162
2007 NB1 mediates surface expression of the ANCA antigen proteinase 3 on human neutrophils. Blood 101 17244676
1990 Biochemical characterization of the neutrophil-specific antigen NB1. Blood 91 2153425
2005 The Jak2V617F mutation, PRV-1 overexpression, and EEC formation define a similar cohort of MPD patients. Blood 87 15985544
2010 Complement receptor Mac-1 is an adaptor for NB1 (CD177)-mediated PR3-ANCA neutrophil activation. The Journal of biological chemistry 82 21193407
2001 Molecular basis of the neutrophil glycoprotein NB1 (CD177) involved in the pathogenesis of immune neutropenias and transfusion reactions. European journal of immunology 79 11465086
2021 CD177 modulates the function and homeostasis of tumor-infiltrating regulatory T cells. Nature communications 78 34599187
2020 Generation of pancreatic β cells from CD177+ anterior definitive endoderm. Nature biotechnology 77 32341565
2013 Higgs amplitude mode in the BCS superconductors Nb1-xTi(x)N induced by terahertz pulse excitation. Physical review letters 76 23952432
1994 Early tagma-specific commitment of Drosophila CNS progenitor NB1-1. Development (Cambridge, England) 75 7956832
2003 Quantification of PRV-1 mRNA distinguishes polycythemia vera from secondary erythrocytosis. Blood 72 12893745
2005 Concomitant neutrophil JAK2 mutation screening and PRV-1 expression analysis in myeloproliferative disorders and secondary polycythaemia. British journal of haematology 70 16197445
2009 Coexpression of CD177 and membrane proteinase 3 on neutrophils in antineutrophil cytoplasmic autoantibody-associated systemic vasculitis: anti-proteinase 3-mediated neutrophil activation is independent of the role of CD177-expressing neutrophils. Arthritis and rheumatism 69 19404956
2004 CD177: A member of the Ly-6 gene superfamily involved with neutrophil proliferation and polycythemia vera. Journal of translational medicine 69 15050027
2004 Neutrophil CD177 (NB1 gp, HNA-2a) expression is increased in severe bacterial infections and polycythaemia vera. British journal of haematology 67 15238147
2007 Neutrophil-specific antigen HNA-2a, NB1 glycoprotein, and CD177. Current opinion in hematology 66 17898576
2010 Neutrophil transmigration mediated by the neutrophil-specific antigen CD177 is influenced by the endothelial S536N dimorphism of platelet endothelial cell adhesion molecule-1. Journal of immunology (Baltimore, Md. : 1950) 64 20194726
2012 Proteinase 3 contributes to transendothelial migration of NB1-positive neutrophils. Journal of immunology (Baltimore, Md. : 1950) 63 22266279
2016 Identification of CD177 as the most dysregulated parameter in a microarray study of purified neutrophils from septic shock patients. Immunology letters 60 27568821
2000 Variations in the expression of granulocyte antigen NB1. Transfusion 60 10864984
2004 PRV-1 mRNA expression discriminates two types of essential thrombocythemia. Annals of hematology 55 15034760
2008 A hydrophobic patch on proteinase 3, the target of autoantibodies in Wegener granulomatosis, mediates membrane binding via NB1 receptors. The Journal of biological chemistry 53 18854317
2013 IL-17-producing peripheral blood CD177+ neutrophils increase in allergic asthmatic subjects. Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology 52 23822853
2002 Modulation of neuritogenesis by ganglioside-specific sialidase (Neu 3) in human neuroblastoma NB-1 cells. Neurochemical research 51 12374221
2010 Elevated neutrophil membrane expression of proteinase 3 is dependent upon CD177 expression. Clinical and experimental immunology 48 20491791
2015 Genetic mapping of the nulliplex-branch gene (gb_nb1) in cotton using next-generation sequencing. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 47 25575840
2002 Biochemical characterization of PRV-1, a novel hematopoietic cell surface receptor, which is overexpressed in polycythemia rubra vera. Blood 47 12239154
2022 CD177+ cells produce neutrophil extracellular traps that promote biliary atresia. Journal of hepatology 46 35803543
2010 Comparison of human islet isolation outcomes using a new mammalian tissue-free enzyme versus collagenase NB-1. Transplantation 45 20463640
2003 Lack of NB1 GP (CD177/HNA-2a) gene transcription in NB1 GP- neutrophils from NB1 GP-expressing individuals and association of low expression with NB1 gene polymorphisms. Blood 43 12623849
2014 Characterization of a novel mouse model with genetic deletion of CD177. Protein & cell 42 25359465
2002 Molecular basis of NB1 (HNA-2a, CD177) deficiency. Blood 42 12010833
2014 Differential expression of granulopoiesis related genes in neutrophil subsets distinguished by membrane expression of CD177. PloS one 41 24926686
2012 Deregulation of apoptosis-related genes is associated with PRV1 overexpression and JAK2 V617F allele burden in Essential Thrombocythemia and Myelofibrosis. Journal of hematology & oncology 41 22300941
2017 Evaluation of 11C-Me-NB1 as a Potential PET Radioligand for Measuring GluN2B-Containing NMDA Receptors, Drug Occupancy, and Receptor Cross Talk. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 39 29191857
2017 Gene silencing and a novel monoallelic expression pattern in distinct CD177 neutrophil subsets. The Journal of experimental medicine 38 28559244
2018 CD177-mediated nanoparticle targeting of human and mouse neutrophils. PloS one 37 29990379
2016 Heterogeneity of Human Neutrophil CD177 Expression Results from CD177P1 Pseudogene Conversion. PLoS genetics 32 27227454
2009 Age- and division-of-labour-dependent differential expression of a novel non-coding RNA, Nb-1, in the brain of worker honeybees, Apis mellifera L. Insect molecular biology 32 19817910
2009 Large-scale comparison of Liberase HI and collagenase NB1 utilized for human islet isolation. Cell transplantation 32 19818208
2004 Clinical significance of neutrophil CD177 mRNA expression in Ph-negative chronic myeloproliferative disorders. British journal of haematology 32 15327515
1996 Analysis of the expression of NB1 antigen using two monoclonal antibodies. Transfusion 32 8614969
2009 Molecular studies reveal that A134T, G156A and G1333A SNPs in the CD177 gene are associated with atypical expression of human neutrophil antigen-2. Vox sanguinis 31 19695014
2003 Neutrophil PRV-1 expression across the chronic myeloproliferative disorders and in secondary or spurious polycythemia. Blood 31 14701686
1991 Neutrophil-specific antigen NB1 is anchored via a glycosyl-phosphatidylinositol linkage. Journal of leukocyte biology 31 1825110
2020 The neutrophil subset defined by CD177 expression is preferentially recruited to gingival crevicular fluid in periodontitis. Journal of leukocyte biology 30 32531826
2015 Capability of exopolysaccharide-producing Lactobacillus paraplantarum BGCG11 and its non-producing isogenic strain NB1, to counteract the effect of enteropathogens upon the epithelial cell line HT29-MTX. Food research international (Ottawa, Ont.) 28 28411984
2003 Expression of human neutrophil antigen-2a (NB1) is increased in pregnancy. Transfusion 28 12675722
2019 Preclinical Evaluation of Benzazepine-Based PET Radioligands (R)- and (S)-11C-Me-NB1 Reveals Distinct Enantiomeric Binding Patterns and a Tightrope Walk Between GluN2B- and σ1-Receptor-Targeted PET Imaging. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 26 30683765
2006 The gene overexpressed in polycythemia rubra vera, PRV-1, and the gene encoding a neutrophil alloantigen, NB1, are alleles of a single gene, CD177, in chromosome band 19q13.31. Transfusion 26 16533288
2021 Triggering Receptor Expressed on Myeloid Cells-1 Agonist Regulates Intestinal Inflammation via Cd177+ Neutrophils. Frontiers in immunology 25 33767714
2019 Increase expression of CD177 in Kawasaki disease. Pediatric rheumatology online journal 25 30943984
2002 The use of bioinformatics to identify the genomic structure of the gene that encodes neutrophil antigen NB1, CD177. Clinical immunology (Orlando, Fla.) 25 11846455
1998 The expression of the NB1 antigen on myeloid precursors and neutrophils from children and umbilical cords. Transfusion medicine (Oxford, England) 25 9675788
2020 Consequences of Piscine orthoreovirus genotype 1 (PRV-1) infections in Chinook salmon (Oncorhynchus tshawytscha), coho salmon (O. kisutch) and rainbow trout (O. mykiss). Journal of fish diseases 24 32476167
1987 Biological properties of a tumour cell line (NB1-G) derived from human neuroblastoma. British journal of cancer 24 2437946
2020 Screening of Fish Cell Lines for Piscine Orthoreovirus-1 (PRV-1) Amplification: Identification of the Non-Supportive PRV-1 Invitrome. Pathogens (Basel, Switzerland) 23 33053677
2001 Immune neutropenia associated with anti-human neutrophil antigen-2a (NB1) antibodies following unrelated donor stem cell transplantation for chronic myeloid leukaemia: perpetuation by granulocyte colony-stimulating factor. British journal of haematology 22 11380420
1993 Quinine-dependent antibodies to neutrophils react with a 60-Kd glycoprotein on which neutrophil-specific antigen NB1 is located and an 85-Kd glycosyl-phosphatidylinositol-linked N-glycosylated plasma membrane glycoprotein. Blood 22 8387837
2021 First-in-Humans Brain PET Imaging of the GluN2B-Containing N-methyl-d-aspartate Receptor with (R)-11C-Me-NB1. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 21 34620732
2020 Preclinical Development of 18F-OF-NB1 for Imaging GluN2B-Containing N-Methyl-d-Aspartate Receptors and Its Utility as a Biomarker for Amyotrophic Lateral Sclerosis. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 21 32737247
2003 Increased risk for vascular complications in PRV-1 positive patients with essential thrombocythaemia. British journal of haematology 21 14617016
2023 Dysregulation of CD177+ neutrophils on intraepithelial lymphocytes exacerbates gut inflammation via decreasing microbiota-derived DMF. Gut microbes 19 36729914
2021 Inflammatory signature in acute-on-chronic liver failure includes increased expression of granulocyte genes ELANE, MPO and CD177. Scientific reports 19 34552111
2004 Significance of human neutrophil antigen-2a (NB1) expression and neutrophil number in pregnancy. Transfusion 19 15043575
2004 Serva collagenase NB1: a new enzyme preparation for human islet isolation. Transplantation proceedings 19 15194398
2003 The presence of a significant association between elevated PRV-1 mRNA expression and low plasma erythropoietin concentration in essential thrombocythaemia. European journal of haematology 19 12756017
2021 PRV-1 Infected Macrophages in Melanized Focal Changes in White Muscle of Atlantic Salmon (Salmo salar) Correlates With a Pro-Inflammatory Environment. Frontiers in immunology 18 33995395
2016 A sequence-specific polymerase chain reaction method for HNA-2 genotyping: homozygous c.843A>T mutation predicts the absence of CD177. Transfusion 18 27385476
2013 CD177 expression on neutrophils: in search of a clonal assay for myeloid neoplasia by flow cytometry. American journal of clinical pathology 18 24124144
2012 Comparative evaluation of the role of the adhesion molecule CD177 in neutrophil interactions with platelets and endothelium. European journal of haematology 18 22690867
2007 Phenoxazine derivatives 2-amino-4,4alpha-dihydro-4alpha-phenoxazine-3-one and 2-aminophenoxazine-3-one-induced apoptosis through a caspase-independent mechanism in human neuroblastoma cell line NB-1 cells. Biological & pharmaceutical bulletin 18 17268075
2004 Platelet-rich plasma serotonin levels in chronic myeloproliferative disorders: evaluation of diagnostic use and comparison with the neutrophil PRV-1 assay. British journal of haematology 18 15384975
2007 Biomarker analysis in polycythemia vera under interferon-alpha treatment: clonality, EEC, PRV-1, and JAK2 V617F. Annals of hematology 17 17256145
2004 PRV-1, erythroid colonies and platelet Mpl are unrelated to thrombosis in essential thrombocythaemia. British journal of haematology 16 15461629
2024 Dual role of CD177 + neutrophils in inflammatory bowel disease: a review. Journal of translational medicine 15 39223577
2020 CD177 Enhances the Detection of Myelodysplastic Syndrome by Flow Cytometry. American journal of clinical pathology 15 32011681
2020 Cancer cell-intrinsic function of CD177 in attenuating β-catenin signaling. Oncogene 15 32042113
2019 The nonconservative CD177 single-nucleotide polymorphism c.1291G>A is a genetic determinant for human neutrophil antigen-2 atypical/low expression and deficiency. Transfusion 15 30828823
2018 Interaction between CD177 and platelet endothelial cell adhesion molecule-1 downregulates membrane-bound proteinase-3 (PR3) expression on neutrophils and attenuates neutrophil activation induced by PR3-ANCA. Arthritis research & therapy 15 30236159
1993 Polyclonal antibodies against the NB1-bearing 58- to 64-kDa glycoprotein of human neutrophils do not identify an NB2-bearing molecule. Transfusion 15 8488543
2020 Dissemination of Piscine orthoreovirus-1 (PRV-1) in Atlantic Salmon (Salmo salar) during the Early and Regenerating Phases of Infection. Pathogens (Basel, Switzerland) 14 32093243
2005 Inhibition of thrombin-induced vascular endothelial growth factor production in human neuroblastoma (NB-1) cells by argatroban. Pathophysiology of haemostasis and thrombosis 14 16293985
1994 Neutrophil-specific antigen NB1 inhibits neutrophil-endothelial cell interactions. The Journal of laboratory and clinical medicine 14 8301201
2022 Evaluation of (rac)-, (R)-, and (S)-18F-OF-NB1 for Imaging GluN2B Subunit-Containing N-Methyl-d-Aspartate Receptors in Nonhuman Primates. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 13 35710735
2021 Piscine Orthoreovirus (PRV)-3, but Not PRV-2, Cross-Protects against PRV-1 and Heart and Skeletal Muscle Inflammation in Atlantic Salmon. Vaccines 13 33800725
2021 The neutrophil protein CD177 is a novel PDPN receptor that regulates human cancer-associated fibroblast physiology. PloS one 13 34879110
2019 Kidney injury enhances renal G-CSF expression and modulates granulopoiesis and human neutrophil CD177 in vivo. Clinical and experimental immunology 13 31509227
2015 MnSOD downregulation induced by extremely low 0.1 mGy single and fractionated X-rays and microgravity treatment in human neuroblastoma cell line, NB-1. Journal of clinical biochemistry and nutrition 13 26388666
1997 Synthesis and purification of NB1-palmitoyl insulin. Journal of pharmaceutical sciences 13 9383737
1993 Analysis of the expression of neutrophil-specific antigen NB1: characterization of neutrophils that react with but are not agglutinated by anti-NB1. Transfusion 13 8342232
2024 Nb1.60Ti0.32W0.08O5-δ as negative electrode active material for durable and fast-charging all-solid-state Li-ion batteries. Nature communications 12 39396046
2019 Reflection of neuroblastoma intratumor heterogeneity in the new OHC-NB1 disease model. International journal of cancer 12 31304977