Affinage

ARID5A

AT-rich interactive domain-containing protein 5A · UniProt Q03989

Round 2 corrected
Length
594 aa
Mass
64.1 kDa
Annotated
2026-04-28
67 papers in source corpus 23 papers cited in narrative 23 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

ARID5A is a bifunctional nucleic acid-binding protein that acts as both a post-transcriptional regulator of proinflammatory and metabolic mRNAs and a context-dependent transcriptional co-activator or repressor. In the cytoplasm, the ARID domain—extended by intrinsically disordered regions—recognizes AU-rich stem-loop structures in the 3′UTRs of target mRNAs (IL-6, STAT3, T-bet, OX40, IDO1, MAVS), stabilizing them against Regnase-1- and Roquin-mediated decay, and associates with 18S rRNA to promote translation of targets such as C/EBPβ and C/EBPδ (PMID:23676272, PMID:27022145, PMID:27671645, PMID:29244194, PMID:39058386, PMID:40301689). In the nucleus, ARID5A cooperates with Sox9 to activate Col2a1 transcription in chondrocytes and represses PPARγ2, CD36, and FABP4 to restrain adipogenesis and muscle lipid uptake (PMID:21346191, PMID:31289228, PMID:40632872). ARID5A expression is induced by NF-κB, noncanonical pSTAT1, and CREB, while its turnover is governed by p38 MAPK-dependent phosphorylation and WWP1-mediated K48-linked ubiquitination; stimulus-dependent nuclear–cytoplasmic shuttling via importin-α/β1 import and CRM1-dependent export enables its dual functions (PMID:28168301, PMID:29358370, PMID:32209697).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 2005 High

    Before its RNA-binding role was recognized, ARID5A (MRF1) was identified as a nuclear receptor corepressor, establishing that it directly interacts with ERα and other nuclear receptors to repress transcription independently of HDACs.

    Evidence Yeast two-hybrid, co-IP, and reporter assays in transfected cells

    PMID:15941852

    Open questions at the time
    • Endogenous target genes of the ERα–ARID5A repressor complex were not identified
    • In vivo relevance of nuclear receptor corepression was not tested
  2. 2011 High

    Discovery of ARID5A as a transcriptional co-activator of Sox9 at the Col2a1 promoter revealed that its nuclear function is context-dependent—activating or repressing transcription depending on partner factors.

    Evidence Co-IP with Sox9, ChIP at Col2a1, overexpression/knockdown in ATDC5 chondrocytes

    PMID:21346191

    Open questions at the time
    • Whether ARID5A binds Col2a1 DNA directly via its ARID domain or is recruited indirectly through Sox9 was not resolved
    • Genome-wide transcriptional co-activator targets in chondrocytes were not mapped
  3. 2013 High

    The landmark finding that ARID5A stabilizes IL-6 mRNA by binding its 3′UTR and antagonizing Regnase-1 reframed the protein as a post-transcriptional immune regulator, explaining its requirement for elevated IL-6 during endotoxemia.

    Evidence RNA binding assays, Arid5a-deficient mice, LPS challenge, mRNA stability assays

    PMID:23676272

    Open questions at the time
    • Structural basis of the 3′UTR interaction was unknown
    • Full repertoire of stabilized mRNAs remained undefined
  4. 2016 High

    Extension of the mRNA stabilization paradigm to STAT3 and T-bet mRNAs demonstrated that ARID5A selectively governs Th17 and Th1 differentiation programs by stabilizing lineage-defining transcription factor transcripts.

    Evidence Arid5a KO Th17/Th1 polarization assays, stem-loop binding, LPS/P. acnes septic shock models

    PMID:27022145 PMID:27671645

    Open questions at the time
    • How ARID5A discriminates STAT3 from STAT1/STAT5 mRNAs at the structural level was not defined
    • Relative contribution of Th17 vs Th1 arm in autoimmunity was unresolved
  5. 2017 High

    Identification of the NF-κB–driven induction and p38 MAPK/WWP1-mediated K48-ubiquitination of ARID5A established the negative feedback loop that terminates its activity during the late phase of inflammation.

    Evidence Phosphorylation/ubiquitination assays, mutagenesis, NF-κB/MAPK inhibitors, AUF-1 mRNA decay

    PMID:28168301

    Open questions at the time
    • Specific p38-phosphorylated residues on ARID5A were not mapped at single-site resolution
    • Whether other E3 ligases contribute to ARID5A turnover was not tested
  6. 2018 High

    Three simultaneous advances broadened ARID5A's mechanism: (i) nuclear–cytoplasmic shuttling via importin-α/β1 and CRM1 was shown to be required for its dual functions; (ii) OX40 mRNA was identified as a target stabilized against both Regnase-1 and Roquin; (iii) ARID5A was found to associate with the translation initiation machinery to promote C/EBPβ translation downstream of IL-17.

    Evidence Importin/CRM1 inhibitors in transgenic mice; stem-loop mutagenesis and EAE model; TRAF2 co-IP and polysome association

    PMID:29244194 PMID:29358370 PMID:30301788

    Open questions at the time
    • How ARID5A engages the translation initiation complex mechanistically (direct vs indirect) was not resolved
    • Identity of UPF1 interaction's functional consequence remained unclear
  7. 2019 High

    Demonstration that ARID5A represses PPARγ2 transcription to restrain adipogenesis extended its nuclear repressor function to metabolic homeostasis, with knockout mice developing adult-onset obesity.

    Evidence Arid5a KO (obesity) and transgenic (obesity resistance) mice, 3T3-L1 differentiation

    PMID:31289228

    Open questions at the time
    • Whether ARID5A directly binds PPARγ2 regulatory DNA or acts through a cofactor complex was not determined
    • Interaction with chromatin-modifying enzymes at adipogenic loci was not addressed
  8. 2020 High

    Upstream regulation of ARID5A was further defined: noncanonical pSTAT1-Thr749 binds a novel motif in the ARID5A promoter downstream of TLR4 endocytosis, and β2-adrenergic/CREB signaling induces ARID5A in cardiac fibroblasts, revealing tissue-specific transcriptional control.

    Evidence ChIP, site-directed mutagenesis, ARID5A promoter reporters; β2AR agonists and PKA inhibitors in Arid5a KO fibroblasts

    PMID:32209697 PMID:32302067

    Open questions at the time
    • Whether pSTAT1-Thr749 and NF-κB cooperate at the ARID5A promoter simultaneously was not tested
    • In vivo cardiac phenotype of ARID5A induction via β2AR was not examined
  9. 2021 High

    ARID5A's role in tumor immune evasion was established: it stabilizes IDO1 and CCL2 mRNAs in tumor cells, promoting tryptophan catabolism and immunosuppression; separately, it activates transcription of a lncRNA driving breast cancer metastasis.

    Evidence Arid5a KO tumor lines in syngeneic models with immune profiling; Arid5a KO with metastasis assays

    PMID:34006522 PMID:34389433

    Open questions at the time
    • Whether ARID5A's mRNA stabilization vs transcriptional activation roles can be pharmacologically uncoupled was not explored
    • Generalizability across cancer types beyond melanoma, colon, and breast was not tested
  10. 2024 High

    Structural and transcriptome-wide studies resolved how ARID5A recognizes nucleic acids: NMR defined ARID domain–DNA contacts, RBNS/iCLIP2 identified a consensus AU-rich RNA motif, and IDRs flanking the ARID domain were shown to be essential for RNA binding; simultaneously, RIP-Seq and polysome profiling established ARID5A as a ribosome-associated translational regulator via 18S rRNA interaction.

    Evidence NMR, RBNS, iCLIP2, IDR mutagenesis; RIP-Seq, polysome profiling in Arid5a KO mice

    PMID:38866324 PMID:39058386

    Open questions at the time
    • Atomic-resolution structure of ARID5A bound to an RNA stem-loop is still lacking
    • How 18S rRNA association promotes translation of specific mRNAs over others is mechanistically unresolved
  11. 2025 High

    ARID5A's target repertoire was extended to cardiac aging (MAVS mRNA stabilization) and skeletal muscle lipid metabolism (transcriptional repression of CD36/FABP4), demonstrating that its dual nuclear/cytoplasmic functions operate across diverse non-immune tissues.

    Evidence scRNA-seq of human hearts, ARID5A shRNA gene therapy in aged mice; GR knock-in mice, myotropic AAV OE/KD with ChIP-seq

    PMID:40301689 PMID:40632872

    Open questions at the time
    • Whether ARID5A-MAVS stabilization contributes to non-cardiac aging tissues remains untested
    • Structural basis of ARID5A's dual DNA-repressor vs mRNA-stabilizer selectivity is undefined

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the atomic structure of ARID5A in complex with RNA stem-loops, the mechanism by which ribosome association selectively enhances translation of specific targets, and whether the transcriptional and post-transcriptional functions can be pharmacologically dissected for therapeutic intervention.
  • No high-resolution co-crystal or cryo-EM structure of ARID5A bound to RNA
  • Selectivity mechanism for ribosome-associated translational enhancement is unknown
  • No small-molecule inhibitors of ARID5A have been reported

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 10 GO:0003677 DNA binding 4 GO:0140110 transcription regulator activity 4 GO:0045182 translation regulator activity 2 GO:0098772 molecular function regulator activity 2
Localization
GO:0005634 nucleus 5 GO:0005829 cytosol 3
Pathway
R-HSA-8953854 Metabolism of RNA 7 R-HSA-168256 Immune System 6 R-HSA-162582 Signal Transduction 5 R-HSA-74160 Gene expression (Transcription) 5 R-HSA-1430728 Metabolism 3 R-HSA-1266738 Developmental Biology 1
Partners
ESRAREGNASE1ROQUIN1RORGCSOX9TRAF2UPF1WWP1

Evidence

Reading pass · 23 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2013 Arid5a is an RNA-binding protein that stabilizes IL-6 mRNA by binding to its 3' untranslated region, counteracting the destabilizing effect of the ribonuclease Regnase-1, thereby promoting elevation of IL-6 serum levels in vivo. RNA binding assays, Arid5a-deficient mice, LPS challenge model, mRNA stability assays Proceedings of the National Academy of Sciences of the United States of America High 23676272
2011 Arid5a physically interacts with Sox9 in the nucleus, binds directly to the Col2a1 gene promoter, stimulates histone H3 acetylation at that region, and cooperatively enhances chondrocyte-specific transcription and differentiation. Co-immunoprecipitation, ChIP, overexpression and knockdown in ATDC5 cells, organ culture Molecular biology of the cell High 21346191
2016 Arid5a selectively stabilizes Stat3 mRNA (but not Stat1 or Stat5 mRNA) in CD4+ T cells in an IL-6-dependent manner, directing naive CD4+ T cells toward Th17 differentiation; loss of Arid5a reduces STAT3 levels and shifts cells toward IL-10-expressing anti-inflammatory fate. Arid5a-deficient mice, mRNA stability assays, Th17 polarization assays, flow cytometry The Journal of experimental medicine High 27022145
2016 Arid5a binds to a conserved stem-loop structure in the 3'UTR of T-bet mRNA and stabilizes it in Th1 cells, thereby promoting IFN-γ production and contributing to septic shock; Arid5a-deficient mice are resistant to LPS-induced endotoxic shock with reduced IFN-γ and IL-6. RNA binding assays (stem-loop structure), Arid5a KO mice, LPS/P. acnes endotoxic shock models, cytokine measurement Proceedings of the National Academy of Sciences of the United States of America High 27671645
2017 During LPS stimulation, NF-κB activates Arid5a gene expression (promoting IL-6 mRNA stabilization), while in the late phase p38 MAPK phosphorylates Arid5a and recruits the E3 ubiquitin ligase WWP1, which ubiquitinates Arid5a via K48-linked chains leading to its proteasomal degradation; additionally, AUF-1 destabilizes Arid5a mRNA via AU-rich elements in its 3'UTR. Phosphorylation assays, ubiquitination assays, NF-κB/MAPK pathway inhibitors, mutagenesis blocking phosphorylation, mRNA stability assays Nucleic acids research High 28168301
2018 IL-17 signaling induces Arid5a expression; Arid5a is recruited to the adaptor TRAF2, stabilizes IL-17-induced cytokine transcripts by binding their 3'UTRs, counteracts MCPIP1 (Regnase-1)-mediated mRNA degradation, and associates with the eukaryotic translation initiation complex to facilitate translation of the transcription factors IκBζ and C/EBPβ, creating a feed-forward amplification loop. Co-immunoprecipitation (TRAF2, eIF complex), mRNA stability assays, translation assays, Arid5a KO cells Science signaling High 30301788
2018 Arid5a stabilizes OX40 mRNA in CD4+ T cells by recognizing an alternative decay element (ADE)-like stem-loop structure in the OX40 3'UTR, and impairs the RNA-destabilizing functions of both Regnase-1 and Roquin-1 on this target. RNA binding assays, stem-loop mutagenesis, Arid5a KO mice, EAE model, adoptive transfer European journal of immunology High 29244194
2018 Arid5a is present in both cytoplasm and nucleus of resting cells; upon inflammatory stimulation it is imported into the nucleus via the classical importin-α/β1 pathway, and subsequently exported to the cytoplasm via CRM1-dependent nuclear export; cytoplasmic Arid5a is associated with UPF1 (up-frameshift protein 1). This stimulus-dependent nuclear-cytoplasmic shuttling is required for its dual function in mRNA stabilization and transcriptional regulation. Transgenic mice, nuclear fractionation, importin pathway inhibitors (importazole), CRM1 inhibitor (leptomycin B), co-immunoprecipitation with UPF1 Proceedings of the National Academy of Sciences of the United States of America High 29358370
2019 Arid5a represses transcription of Ppar-γ2, acting as a negative regulator of adipogenesis; in the absence of Arid5a, persistent Ppar-γ2 expression drives adipocyte differentiation and enhanced fatty acid uptake. Arid5a and Ppar-γ2 are dynamically counter-regulated by each other to maintain adipose tissue homeostasis. Arid5a KO mice (adult-onset obesity), Arid5a transgenic mice (diet-induced obesity resistance), 3T3-L1 differentiation assays, gene expression analysis Proceedings of the National Academy of Sciences of the United States of America High 31289228
2021 Arid5a stabilizes Ido1 (indoleamine 2,3-dioxygenase 1) and Ccl2 mRNAs in tumor cells, augmenting tryptophan catabolism and creating an immunosuppressive tumor microenvironment that promotes immune evasion; deletion of Arid5a in tumor cells enhances antitumor immunity in immunocompetent but not immunodeficient mice. Arid5a KO tumor cell lines, mRNA stability assays, syngeneic mouse tumor models, immune cell profiling Cancer immunology research High 34006522
2021 IL-6-induced Arid5a promotes transcription of the lncRNA AU021063, which in turn stabilizes Trib3 and activates Mek/Erk signaling to drive breast cancer metastasis; genetic ablation of Arid5a abolishes this cascade. Arid5a KO, RNA stability assays, in vitro invasion assays, mouse metastasis models Cancer letters Medium 34389433
2005 MRF1 (ARID5A) binds to both the N-terminal and C-terminal regions of ERα in an estradiol-independent manner (also interacts with thyroid receptor α, RXRα, and androgen receptor in ligand-dependent manner), has intrinsic repressor activity, and represses ERα-mediated transcriptional activation in a dose-dependent manner without requiring histone deacetylase activity. Yeast two-hybrid screen, co-immunoprecipitation, recombinant protein binding assays, transient transfection reporter assays, GAL4 reporter assay Molecular endocrinology High 15941852
2014 ARID5A is induced by IL-6 in CD4+ T cells via a STAT3-dependent mechanism; ARID5A physically associates with RORγt through its N-terminal region and acts as a negative regulator of RORγt-induced Th17 cell differentiation and IL-17A promoter activation. Co-immunoprecipitation, overexpression in murine CD4+ T cells, IL-17A promoter reporter assay, flow cytometry Arthritis & rheumatology Medium 24782182
2020 Noncanonical phosphorylation of STAT1 at Thr749 (mediated by a TBK1-IKKβ complex downstream of TLR4 endocytosis) drives STAT1 binding to a noncanonical DNA motif (5'-TTTGANNC-3') in the ARID5A promoter, thereby activating ARID5A expression and downstream IL-6 mRNA stabilization. Site-specific mutagenesis, ChIP, kinase inhibitors, TLR4 endocytosis manipulation, ARID5A promoter reporter assays Science signaling High 32209697
2020 β-catenin-mediated transcriptional activation of FOSL2 and repression of ARID5A together drive M1-to-M2 TAM polarization in lung cancer; pharmacological or genetic ablation of β-catenin reprograms M2-like TAMs to M1-like TAMs and suppresses tumor growth. Pharmacological β-catenin inhibition, macrophage-specific genetic ablation, transcriptome analysis, in vitro TAM model, in vivo lung tumor models Science advances Medium 32548260
2024 Using NMR-centered biochemistry, the Arid5a ARID domain was found to bind DNA with a defined preference; high-throughput in vitro binding defined a consensus RNA-binding motif engaged by the core ARID domain; iCLIP2 revealed transcriptome-wide binding preference for (A)U-rich regions in pre-mRNA transcripts; intrinsically disordered regions (IDRs) flanking the ARID domain modulate DNA-binding specificity and affinity, and are crucial for RNA interactions. NMR spectroscopy, high-throughput in vitro binding (RBNS), iCLIP2, mutagenesis of IDR extensions The Journal of biological chemistry High 38866324
2024 ARID5A stabilizes IDO1 mRNA in colorectal cancer cells, leading to upregulation of IDO1 expression, tryptophan-to-kynurenine conversion, kynurenine-mediated AhR activation in CAR-T cells, and consequent CAR-T cell exhaustion; targeting the ARID5A-IDO1-AhR axis with AhR or IDO1 inhibitors alleviates T cell exhaustion. ARID5A overexpression, mRNA stability assays, CAR-T co-culture assays, AhR/IDO1 inhibitor treatment Translational oncology Medium 38316094
2024 Transcriptome-wide RIP-Seq revealed that Arid5a inducibly interacts with IL-17 target mRNAs including CEBPB and CEBPD, as well as rRNAs including 18S rRNA (a 40S ribosome constituent); IL-17 promotes Arid5a nuclear export and association with 18S rRNA; Arid5a-deficient cells show repressed global protein synthesis, with C/EBPs controlled at the translational rather than mRNA level, establishing Arid5a as a ribosome-associated translational regulator. RIP-Seq, Arid5a KO mice, AGN mouse model, fractionation, polysome profiling, CRISPR-Cas9 KO The Journal of experimental medicine High 39058386
2025 ARID5A epi-transcriptionally stabilizes MAVS (Mitochondrial Antiviral Signaling Protein) mRNA in cardiomyocytes of aged hearts, leading to NF-κB and TBK1 activation and amplifying cardiac aging and inflammation; lentiviral shRNA targeting ARID5A in aged mouse myocardium mitigated inflammatory and aging phenotypes and improved cardiac function. Single-cell RNA-seq of human heart tissues, ARID5A-MAVS RNA binding/stability assays, lentiviral shRNA gene therapy in aged mice, NF-κB/TBK1 pathway analysis Nature cardiovascular research High 40301689
2025 In skeletal muscle, Arid5a functions as a transcriptional repressor of the lipid uptake genes Cd36 and Fabp4, downstream of glucocorticoid receptor (GR) transactivation; Arid5a is required and sufficient to reduce muscle triacylglycerol accumulation, acting as a pro-metabolic effector. GR knock-in mice (rs6190), myotropic AAV overexpression/knockdown in muscle, RNA-seq and ChIP-seq, metabolic phenotyping Science advances Medium 40632872
2026 TNF upregulates Arid5a expression through the NF-κB1/TRAF2 pathway, causing cytoplasmic relocalization of Arid5a; Arid5a then stabilizes proinflammatory transcripts and enhances expression of chemokines driving rheumatoid arthritis; Arid5a-deficient mice are resistant to collagen-induced arthritis with reduced Th17 cells in synovial tissue. TRAF2 KO cells, NF-κB/IKK inhibitors, NIK inhibitors, Arid5a KO mice, collagen-induced arthritis model, mRNA stability assays JCI insight High 41574607
2022 Rbpjl binds to the promoter region of Arid5a and transcriptionally represses its expression, thereby suppressing the Arid5a/IL-6/STAT3 signaling axis and alleviating pancreatic acinar cell inflammation in acute pancreatitis. ChIP, EMSA, dual-luciferase reporter assays, Rbpjl overexpression/knockdown, LPS-induced cell model, cerulein-induced AP mouse model Cell & bioscience Medium 35725649
2020 β2-adrenergic receptor stimulation activates the cAMP/PKA/CREB pathway in cardiac fibroblasts, phosphorylating CREB and inducing Arid5a expression; Arid5a in turn stabilizes IL-6 mRNA, and this pathway is required for β2AR-mediated IL-6 production (abolished in Arid5a KO cardiac fibroblasts). Arid5a KO cardiac fibroblasts, β2AR agonists, adenylate cyclase activators, PKA inhibitors, CREB activity assays, ELISA Pharmacology research & perspectives Medium 32302067

Source papers

Stage 0 corpus · 67 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2014 A proteome-scale map of the human interactome network. Cell 977 25416956
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2008 Global analysis of host-pathogen interactions that regulate early-stage HIV-1 replication. Cell 787 18854154
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2006 A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Cell 610 16713569
1996 Normalization and subtraction: two approaches to facilitate gene discovery. Genome research 401 8889548
2003 The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Genome research 285 12975309
2011 Next-generation sequencing to generate interactome datasets. Nature methods 200 21516116
2005 DNA-binding properties of ARID family proteins. Nucleic acids research 200 15640446
2013 Arid5a controls IL-6 mRNA stability, which contributes to elevation of IL-6 level in vivo. Proceedings of the National Academy of Sciences of the United States of America 180 23676272
2020 Reprogramming of tumor-associated macrophages by targeting β-catenin/FOSL2/ARID5A signaling: A potential treatment of lung cancer. Science advances 157 32548260
2017 TLR4-induced NF-κB and MAPK signaling regulate the IL-6 mRNA stabilizing protein Arid5a. Nucleic acids research 155 28168301
2007 Toward a confocal subcellular atlas of the human proteome. Molecular & cellular proteomics : MCP 114 18029348
2018 Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei. Molecular & cellular proteomics : MCP 101 30021884
1996 Repression by a differentiation-specific factor of the human cytomegalovirus enhancer. Nucleic acids research 81 8649988
2014 Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation. Cell reports 80 24981860
2020 Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains. Cell reports 79 32814053
2016 Arid5a regulates naive CD4+ T cell fate through selective stabilization of Stat3 mRNA. The Journal of experimental medicine 78 27022145
2018 IL-17 integrates multiple self-reinforcing, feed-forward mechanisms through the RNA binding protein Arid5a. Science signaling 69 30301788
1998 Upregulation of a new microglial gene, mrf-1, in response to programmed neuronal cell death and degeneration. The Journal of neuroscience : the official journal of the Society for Neuroscience 69 9698327
2015 Phospho-tyrosine dependent protein-protein interaction network. Molecular systems biology 61 25814554
2010 Genome-wide association study of theta band event-related oscillations identifies serotonin receptor gene HTR7 influencing risk of alcohol dependence. American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics 60 21184583
2016 Arid5a exacerbates IFN-γ-mediated septic shock by stabilizing T-bet mRNA. Proceedings of the National Academy of Sciences of the United States of America 52 27671645
2011 Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription. Molecular biology of the cell 52 21346191
2018 Regulation of inflammatory responses by dynamic subcellular localization of RNA-binding protein Arid5a. Proceedings of the National Academy of Sciences of the United States of America 50 29358370
2020 Arid5a, an RNA-Binding Protein in Immune Regulation: RNA Stability, Inflammation, and Autoimmunity. Trends in immunology 48 32035762
2018 Arid5a stabilizes OX40 mRNA in murine CD4+ T cells by recognizing a stem-loop structure in its 3'UTR. European journal of immunology 41 29244194
2020 Noncanonical STAT1 phosphorylation expands its transcriptional activity into promoting LPS-induced IL-6 and IL-12p40 production. Science signaling 38 32209697
2014 AT-rich-interactive domain-containing protein 5A functions as a negative regulator of retinoic acid receptor-related orphan nuclear receptor γt-induced Th17 cell differentiation. Arthritis & rheumatology (Hoboken, N.J.) 37 24782182
2021 Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer. Nature communications 36 34373451
2019 Arid5a Regulation and the Roles of Arid5a in the Inflammatory Response and Disease. Frontiers in immunology 34 31867000
2021 The novel long noncoding RNA AU021063, induced by IL-6/Arid5a signaling, exacerbates breast cancer invasion and metastasis by stabilizing Trib3 and activating the Mek/Erk pathway. Cancer letters 27 34389433
2019 Feedback regulation of Arid5a and Ppar-γ2 maintains adipose tissue homeostasis. Proceedings of the National Academy of Sciences of the United States of America 26 31289228
2005 Modulator recognition factor 1, an AT-rich interaction domain family member, is a novel corepressor for estrogen receptor alpha. Molecular endocrinology (Baltimore, Md.) 24 15941852
2021 Arid5a Promotes Immune Evasion by Augmenting Tryptophan Metabolism and Chemokine Expression. Cancer immunology research 22 34006522
2023 Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins. Cell reports 20 36724073
2018 A Potential Therapeutic Target RNA-binding Protein, Arid5a for the Treatment of Inflammatory Disease Associated with Aberrant Cytokine Expression. Current pharmaceutical design 19 29701145
2020 β2-adrenergic stimulation induces interleukin-6 by increasing Arid5a, a stabilizer of mRNA, through cAMP/PKA/CREB pathway in cardiac fibroblasts. Pharmacology research & perspectives 17 32302067
2017 Arid5a-deficient mice are highly resistant to bleomycin-induced lung injury. International immunology 16 28379390
2012 Development and application of a DNA microarray-based yeast two-hybrid system. Nucleic acids research 16 23275563
2002 ATP selectively suppresses the synthesis of the inflammatory protein microglial response factor (MRF)-1 through Ca(2+) influx via P2X(7) receptors in cultured microglia. Brain research 16 12363408
1993 Single point mutations in domain II of the yeast mitochondrial release factor mRF-1 affect ribosome binding. Nucleic acids research 15 8265342
2022 Recent Advances in the Role of Arid5a in Immune Diseases and Cancer. Frontiers in immunology 13 35126382
2020 Differential modulation of Ahr and Arid5a: A promising therapeutic strategy for autoimmune encephalomyelitis. Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society 11 33424253
2002 Selective inflammatory stimulations enhance release of microglial response factor (MRF)-1 from cultured microglia. Glia 11 12420315
2024 ARID5A stabilizes Indoleamine 2,3-dioxygenase expression and enhances CAR T cell exhaustion in colorectal cancer. Translational oncology 10 38316094
2024 Arid5a uses disordered extensions of its core ARID domain for distinct DNA- and RNA-recognition and gene regulation. The Journal of biological chemistry 10 38866324
2022 The emerging role of Arid5a in cancer: A new target for tumors. Genes & diseases 10 37396543
2017 Differentially regulated gene expression in quiescence versus senescence and identification of ARID5A as a quiescence associated marker. Journal of cellular physiology 9 29044508
1994 Isolation and characterization of MRF-1, a brain-derived DNA-binding protein with a capacity to regulate expression of myelin basic protein gene. The Journal of biological chemistry 9 7527040
2022 Blockade of the Arid5a/IL-6/STAT3 axis underlies the anti-inflammatory effect of Rbpjl in acute pancreatitis. Cell & bioscience 8 35725649
2022 Arid5a Mediates an IL-17-Dependent Pathway That Drives Autoimmunity but Not Antifungal Host Defense. Journal of immunology (Baltimore, Md. : 1950) 8 35940634
2025 ARID5A orchestrates cardiac aging and inflammation through MAVS mRNA stabilization. Nature cardiovascular research 6 40301689
2024 The RNA binding protein Arid5a drives IL-17-dependent autoantibody-induced glomerulonephritis. The Journal of experimental medicine 6 39058386
2022 RAGE deficiency ameliorates autoimmune hepatitis involving inhibition of IL-6 production via suppressing protein Arid5a in mice. Clinical and experimental medicine 5 36454447
2023 Arid5a/IL-6/PAI-1 Signaling Is Involved in the Pathogenesis of Lipopolysaccharide-Induced Kidney Injury. Biological & pharmaceutical bulletin 4 38044094
2018 Arid5a makes the IL-17A/F-responsive pathway less arid. Science signaling 4 30301789
2000 Microglial Response Factor (MRF)-1: Constitutive Expression in Ramified Microglia and Upregulation upon Neuronal Death Induced by Ischemia or Glutamate Exposure. Zoological science 4 18517291
2021 Arid5a: A Missing Link between EMT and Tumoral Immune Resistance. Cancer immunology research 3 34158281
2025 Exosome encapsulated miRNA let-7d-3p mediated Zearalenone-induced hepatotoxicity with Arid5a/STAT3 signaling pathway. Journal of hazardous materials 2 40435618
2025 Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids. Nature communications 2 40593736
2025 The human genetic variant rs6190 unveils Foxc1 and Arid5a as novel prometabolic targets of the glucocorticoid receptor in muscle. Science advances 2 40632872
2023 1H, 13C, 15N backbone chemical shift assignments of the extended ARID domain in human AT-rich interactive domain protein 5a (Arid5a). Biomolecular NMR assignments 1 37129704
2026 The RNA binding protein Arid5a is an activator of TNF signaling in rheumatoid arthritis. JCI insight 0 41574607
2025 The human genetic variant rs6190 unveils Foxc1 and Arid5a as novel pro-metabolic targets of the glucocorticoid receptor in muscle. bioRxiv : the preprint server for biology 0 38585940
2025 Centrosome clustering in cancer cells requires microtubule assembly through a RanGTP-dependent TPX2-KIFC1 interaction. Current biology : CB 0 41475341