ANKRD40

ANKRD40 was identified as a component of MZF1 nuclear bodies (MZF1-NBs) through proteomic interactome analysis of these intranuclear structures, where it has been associated with protein folding functions (PMID:39954358). Beyond this single interactome-based association, no direct experimental evidence in the available corpus has established ANKRD40's enzymatic activity, substrates, recruitment mechanism, or defined pathway position.

1. 2025 Low

   Whether ANKRD40 participates in any defined intranuclear assembly was unknown; interactome profiling placed it within MZF1 nuclear bodies and linked it to protein folding functions there.

   Evidence Proteomic interactome analysis of MZF1 nuclear bodies in cells

   PMID:39954358

   Open questions at the time

   • Single lab, single method (interactome listing) without functional validation of ANKRD40's role
   • No reciprocal or direct binding assay confirming the MZF1 interaction
   • The proposed protein folding function is inferred, not demonstrated for ANKRD40

• The molecular activity, direct binding partners, substrates, and pathway context of ANKRD40 remain uncharacterized.
• No enzymatic or biochemical activity established
• No structural model or domain function validated
• Mechanism of recruitment to MZF1 nuclear bodies unknown