| 2020 |
YAF2 specifically binds H2AK119ub1 and recruits the YAF2-PRC1 complex to catalyse ubiquitination of H2A on neighbouring nucleosomes through a positive-feedback model, propagating H2AK119ub1 during cell division; histone H1-compacted chromatin enhances distal propagation of this mark. |
Biochemical binding assays, chromatin fractionation, cell division assays; disruption of RYBP/YAF2-PRC1 activity or H1-dependent compaction caused significant loss of H2AK119ub1 maintenance |
Nature cell biology |
High |
32203418
|
| 2013 |
YAF2 bridges the interaction between YY1 and the PRC1 complex; YAF2 is responsible for PcG recruitment to DNA, which is mediated by YY1 DNA binding. Knock-down of YY1 abrogated PcG recruitment even when exogenous YAF2 was present, showing YY1 DNA binding is a prerequisite for Polycomb assembly. YAF2 and RYBP regulate largely distinct sets of Polycomb target genes. |
Co-IP, ChIP assays in HeLa cells, YY1 knock-down, rescue with exogenous YAF2, dRYBP mutant fly complementation with mouse YAF2 |
Nucleic acids research |
High |
24285299
|
| 2002 |
YAF2 interacts with hGABPβ and YY1 both in vitro and in vivo, and positively regulates transcriptional activity of hGABP, functionally distinct from YEAF1/RYBP which negatively regulates hGABP activity. |
Yeast two-hybrid screening, yeast three-hybrid assay, in vitro and in vivo binding assays, transcriptional reporter assays |
The Journal of biological chemistry |
Medium |
11953439
|
| 2010 |
The YY1 REPO domain (25 amino acids) interacts with YAF2 and recruits YAF2 to DNA; deletion of the REPO domain abolishes this interaction. YAF2, when fused to a heterologous DNA-binding domain, can recruit PcG proteins to DNA and mediate transcriptional repression. Mutation of the Drosophila YAF2 homolog (dRYBP) reduces PcG recruitment to DNA. |
Co-IP, transcriptional repression assays with heterologous DNA-binding domain fusion, deletion mutagenesis of YY1 REPO domain, Drosophila dRYBP mutant analysis |
Journal of cellular biochemistry |
Medium |
19960508
|
| 2006 |
Zebrafish Yaf2 is required for cell survival during embryogenesis; depletion activates widespread caspase 8-mediated apoptosis and causes developmental arrest. Human YAF2 mRNA rescues this phenotype, and YAF2 inhibits caspase 8-mediated apoptosis in cultured cells. |
Morpholino knockdown in zebrafish, caspase inhibitor rescue (pan-caspase and caspase 8-specific), human YAF2 mRNA rescue, apoptosis assays in cultured cells |
The Journal of biological chemistry |
Medium |
16891308
|
| 2001 |
YAF2 binds to the central region of MycN in vitro and in vivo, localizes to the nucleus, and enhances MycN-mediated transactivation from an E-box promoter; deletion of the YAF2-binding region in MycN abrogates this enhancement. |
Yeast two-hybrid, in vitro binding assay, in vivo co-immunoprecipitation, nuclear localization by cell imaging, E-box reporter transactivation assay, deletion mutagenesis of MycN |
Oncogene |
Medium |
11593398
|
| 2003 |
YAF2 binds to the Myc protein in vivo and in vitro, but in contrast to its activating effect on MycN, YAF2 inhibits Myc-mediated transactivation and transformation. |
In vitro binding, in vivo co-immunoprecipitation, transcriptional reporter assays, transformation assays |
Cancer letters |
Medium |
12706874
|
| 2003 |
Mouse YAF2 interacts with Ring1B (and Ring1A), a constituent of mammalian PcG complexes; biochemical and colocalization evidence in tissue culture cells supports YAF2 involvement in PcG complexes together with Ring1B/Ring1A. |
Co-immunoprecipitation, colocalization studies in tissue culture cells, identification of two YAF2 isoforms by alternative splicing |
Gene |
Medium |
14557078
|
| 2015 |
YAF2 binds PDCD5 and stabilizes it by inhibiting ubiquitin-dependent proteasomal degradation, thereby promoting TP53 activation during genotoxic stress. YAF2 knockdown reduces PDCD5 protein (not mRNA) levels. YAF2 promotion of TP53 activation is abolished by PDCD5 deletion and restored by wild-type PDCD5 but not by YAF2-interaction-defective PDCD5 mutants. |
Yeast two-hybrid screen, co-IP, siRNA knockdown, ubiquitination assays, genotoxic stress assays (etoposide), apoptosis rescue with PDCD5 mutants |
Biochimica et biophysica acta |
Medium |
25603536
|
| 2018 |
YAF2 assembles into a noncanonical PRC1 complex via a region encompassing amino acid residues 102–150. Serine 166 is a YAF2 phosphorylation site; S166A mutation compromises Ring1B-mediated H2A monoubiquitination and repression of target genes. Yaf2 deletion in mESCs causes compromised proliferation, abnormal differentiation, and de-repression of ectoderm-associated genes. |
Yeast two-hybrid, co-IP, deletion mutagenesis (residues 102–150), phosphorylation site mutagenesis (S166A), genome-wide profiling (ChIP-seq), H2A ubiquitination assays, mESC knockout |
The Journal of biological chemistry |
High |
29959227
|
| 2021 |
YAF2 mediates interaction between YY1 and SIRT6; pulldown assays show YAF2 associates with both YY1 and SIRT6 at a 1:1:1 molar ratio. YAF2 and YY1 accelerate SIRT6-induced H3K9 deacetylation at the TFAM gene upstream region, contributing to age-related mitochondrial downregulation. |
Pulldown assays, protein cross-linking (molar ratio determination), ChIP-qPCR for H3K9 deacetylation, mRNA transfection experiments, SIRT6 inhibitor treatment |
Molecular and cellular biology |
Medium |
33875574
|
| 2021 |
Phosphorylated YAF2 (at Serine 167) inhibits proteasomal degradation of polyubiquitinated FANK1 by binding to the FN3 domain of FANK1 via the amino-terminal region of YAF2, increasing FANK1 stability and thereby inhibiting tumor cell apoptosis in a FANK1-dependent manner. |
Co-IP, siRNA knockdown, proteasome inhibitor assays, domain mapping (FN3 domain of FANK1 binds N-terminus of YAF2), phosphorylation analysis, apoptosis assays |
Biochemical and biophysical research communications |
Medium |
33784512
|
| 2023 |
RYBP and YAF2 have distinct regulatory functions in neural differentiation: Rybp knockout impairs neural differentiation by activating Wnt signaling; Yaf2 knockout promotes neural differentiation and leads to redistribution of RYBP binding with increased RYBP and H2AK119ub enrichment on RYBP-YAF2 co-targeted genes, preventing ectopic derepression of non-neuroectoderm genes. |
Knockout mESC lines (Rybp KO, Yaf2 KO), ChIP-seq for RYBP and H2AK119ub, genome-wide transcriptional profiling, neural differentiation assays |
Nature communications |
High |
37935677
|