Affinage

WDR4

tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 · UniProt P57081

Length
412 aa
Mass
45.5 kDa
Annotated
2026-04-28
41 papers in source corpus 22 papers cited in narrative 22 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

WDR4 is a WD40-repeat scaffold protein with dual roles as the essential non-catalytic subunit of the METTL1–WDR4 N7-methylguanosine (m7G) methyltransferase complex and as a CUL4 E3 ubiquitin ligase substrate adaptor. Within the methyltransferase complex, WDR4 positions METTL1 and the tRNA T-arm for SAM-dependent m7G46 modification of a subset of tRNAs bearing a RAGGU motif; loss of this modification causes ribosome pausing at cognate codons, impaired translation of cell-cycle and signaling transcripts, and defective embryonic stem cell self-renewal and neural differentiation (PMID:36599982, PMID:29983320). Beyond tRNA, METTL1–WDR4 deposits m7G on select miRNAs (e.g., let-7) and internal mRNA sites to regulate their stability and function (PMID:41663759, PMID:40857569). Independently of its methyltransferase role, WDR4 acts as a CUL4 adaptor that ubiquitinates PTPN23 and Arhgap17 to control receptor trafficking and Rac1-driven cell-cycle progression, respectively, and interacts with eIF4E2 to direct selective mRNA translation; biallelic loss-of-function mutations cause microcephalic primordial dwarfism with cerebellar hypoplasia and ciliogenesis defects (PMID:37821451, PMID:36681682, PMID:41315768, PMID:26416026).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 2006 Medium

    Establishing that WDR4 orthologs are essential WD40-repeat proteins required for gametogenesis and cell division resolved the gene's identity as a conserved developmental factor, linked to yeast Trm82 (tRNA methylase subunit).

    Evidence P-element mutagenesis and transgenic rescue of Drosophila Wuho with cytological analysis of germline arrest

    PMID:16762337

    Open questions at the time
    • Mammalian in vivo requirement not yet tested
    • Enzymatic activity of the complex not directly assayed in this system
  2. 2007 High

    Reconstitution of the yeast Trm8–Trm82 (METTL1–WDR4) heterodimer defined the minimal tRNA structural requirements for m7G46 methylation and revealed that both subunits must be co-translated for activity, establishing WDR4 as an obligate assembly partner rather than a mere accessory factor.

    Evidence In vitro methyltransferase assays with truncated/mutant tRNAs and wheat-germ co-translation reconstitution

    PMID:17382321 PMID:18164779

    Open questions at the time
    • Mammalian complex not yet reconstituted
    • Structural basis of WDR4 contribution unknown
  3. 2015 High

    Identifying a disease-causing WDR4 missense mutation that reduces m7G46 tRNA methylation linked the human gene to microcephalic primordial dwarfism and demonstrated functional conservation from yeast to human.

    Evidence Autozygome/exome sequencing in consanguineous families, yeast complementation with mutant WDR4, tRNA methylation assay

    PMID:26416026

    Open questions at the time
    • Mechanism connecting reduced tRNA modification to microcephaly not defined
    • Whether ubiquitin ligase functions also contribute to disease unknown
  4. 2016 High

    Discovery that WDR4 directly modulates FEN1 endonuclease activity at replication forks—stimulating flap cleavage while inhibiting gap cleavage—revealed a methyltransferase-independent role in genome integrity.

    Evidence Reciprocal Co-IP, colocalization with FEN1/PCNA at nascent DNA, in vitro FEN1 activity assays, knockdown in Drosophila/mouse/human cells

    PMID:26751069

    Open questions at the time
    • Structural basis of WDR4–FEN1 interaction unknown
    • Relative contribution of FEN1 vs. tRNA methylation pathways to the KO phenotype not resolved
  5. 2018 High

    Genome-wide identification of the 22 tRNAs methylated by METTL1–WDR4, combined with ribosome profiling showing pausing at cognate codons upon knockout, established the complex as a codon-level translational regulator essential for stem cell self-renewal and differentiation.

    Evidence m7G-MeRIP-seq, TRAC-seq, ribosome profiling, Mettl1/Wdr4 KO in mouse ESCs, differentiation assays

    PMID:29983320

    Open questions at the time
    • Specific mRNA targets whose translational impairment causes the differentiation defect not identified
    • Whether internal mRNA m7G also contributes not addressed
  6. 2018 Medium

    WDR4 knockout in MEFs triggers γH2AX accumulation, heterochromatin relaxation, and p53/p21-dependent cell cycle arrest, confirming that WDR4 loss causes DNA damage signaling consistent with the FEN1-modulation model.

    Evidence Tamoxifen-inducible Cre KO in MEFs, immunofluorescence, cell cycle analysis

    PMID:29574139

    Open questions at the time
    • Whether DNA damage is primarily due to FEN1 dysregulation vs. other mechanisms not formally dissected
  7. 2021 High

    Demonstrating that METTL1/WDR4-mediated tRNA m7G modification selectively enhances translation of mRNAs enriched in m7G-decoded codons in cancer cells provided a mechanistic link between epitranscriptomic tRNA modification and oncogenic translation programs.

    Evidence m7G-MeRIP-seq, polysome profiling, catalytic mutagenesis, tumor models

    PMID:34371184

    Open questions at the time
    • Whether m7G-driven codon bias acts additively with other tRNA modifications not tested
  8. 2023 High

    High-resolution crystal and cryo-EM structures of the METTL1–WDR4–tRNA ternary complex resolved how WDR4 scaffolds the tRNA T-arm and METTL1, and showed that the METTL1 N-terminus acts as an allosteric switch coupling SAM binding to catalytic activation, with S27 phosphorylation as a regulatory mechanism.

    Evidence X-ray crystallography, cryo-EM with SAM/SAH, phosphomimetic mutagenesis, cellular complementation

    PMID:36599982 PMID:36599985

    Open questions at the time
    • How post-translational modifications of WDR4 itself regulate activity not addressed
    • Structural basis for mRNA/miRNA m7G modification unknown
  9. 2023 High

    Identification of WDR4 as a CUL4 E3 ligase adaptor that ubiquitinates PTPN23 to suppress EGFR/c-MET lysosomal degradation, and separately ubiquitinates Arhgap17 to activate Rac1 in cerebellar granule neuron progenitors, established a methyltransferase-independent ubiquitin ligase function with distinct substrate specificity in different tissues.

    Evidence Ubiquitylome MS, Co-IP, ubiquitination assays, competing peptide rescue, conditional KO mouse cerebellum, Rac1 activity assay

    PMID:36681682 PMID:37821451

    Open questions at the time
    • Full substrate repertoire of WDR4 as CUL4 adaptor unknown
    • Whether ubiquitin ligase and methyltransferase functions are coordinately regulated not tested
  10. 2024 Medium

    Linking WDR4 deficiency to impaired ciliogenesis through ubiquitin pool depletion (excess proteasomal activity) provided a unifying mechanism connecting the ubiquitin ligase function to the microcephaly phenotype and showed pharmacological rescue by proteasome inhibition.

    Evidence Patient-derived fibroblasts, zebrafish models, proteasome activity assay, ubiquitin supplementation rescue

    PMID:39251572

    Open questions at the time
    • Whether ciliogenesis defect is cell-autonomous in neurons not confirmed
    • Relative contribution of tRNA modification loss vs. ubiquitin depletion to microcephaly not resolved
  11. 2025 Medium

    Discovery that cytoplasmic WDR4 interacts with eIF4E2 to drive selective translation of ABCA1 independently of m7G tRNA modification expanded its translational regulatory repertoire beyond epitranscriptomics.

    Evidence Co-IP (WDR4–eIF4E2), polysome fractionation, cholesterol efflux assay, macrophage polarization assay in HCC

    PMID:41315768

    Open questions at the time
    • Whether eIF4E2 interaction is direct or bridged not structurally resolved
    • Full set of selectively translated mRNAs unknown
  12. 2025 Medium

    Extension of METTL1–WDR4 methyltransferase substrates to miRNAs (let-7) and internal mRNA sites (SCLT1) broadened the complex's epitranscriptomic scope to non-tRNA RNAs with functional consequences for stem cell homeostasis and drug resistance.

    Evidence Drosophila midgut epistasis with TOR/JNK inhibitors, human ortholog rescue including catalytic-dead METTL1; m7G MeRIP-seq of mRNAs with catalytic-dead rescue for SCLT1 stability

    PMID:40857569 PMID:41663759

    Open questions at the time
    • Comprehensive catalog of m7G-modified miRNAs and mRNAs not established
    • Structural basis for mRNA internal m7G recognition unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • How the methyltransferase, ubiquitin ligase, and eIF4E2-mediated translational functions of WDR4 are partitioned across subcellular compartments and developmental contexts, and how they individually contribute to the microcephalic primordial dwarfism phenotype, remains unresolved.
  • No separation-of-function allele distinguishing methyltransferase from ubiquitin ligase roles in vivo
  • Structural basis of CUL4 adaptor function not determined
  • Whether WDR4 disease mutations selectively impair one function over others not systematically tested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 6 GO:0060090 molecular adaptor activity 5 GO:0098772 molecular function regulator activity 2 GO:0140096 catalytic activity, acting on a protein 2
Localization
GO:0005634 nucleus 3 GO:0005829 cytosol 1
Pathway
R-HSA-8953854 Metabolism of RNA 5 R-HSA-1266738 Developmental Biology 3 R-HSA-1640170 Cell Cycle 3 R-HSA-1643685 Disease 3 R-HSA-392499 Metabolism of proteins 3 R-HSA-73894 DNA Repair 2
Partners
ARHGAP17DDX20EIF4E2FEN1GSK3BMETTL1PTPN23TRIM32
Complex memberships
CUL4-DDB1-WDR4 E3 ubiquitin ligase complexMETTL1-WDR4 m7G methyltransferase complex

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2023 Crystal structure of METTL1-WDR4 and cryo-EM structures of METTL1-WDR4-tRNA reveal that WDR4 serves as a scaffold for METTL1 and the tRNA T-arm; the composite protein surface recognizes the tRNA elbow through shape complementarity. The METTL1 N-terminus couples cofactor (SAM) binding with conformational changes in the tRNA, the catalytic loop, and the WDR4 C-terminus, acting as the switch to activate m7G methylation. Post-translational modifications of the METTL1 N-terminus can thereby regulate methylation. X-ray crystallography (METTL1-WDR4), cryo-EM (METTL1-WDR4-tRNA with SAM/SAH), active-site structural analysis Nature High 36599982
2023 Structural, biochemical, and cellular studies show WDR4 serves as a scaffold for METTL1 and the tRNA T-arm. Upon tRNA binding, the αC region of METTL1 forms a helix that secures the tRNA variable loop. The predicted disordered N-terminal region of METTL1 is part of the catalytic pocket and essential for methyltransferase activity. S27 phosphorylation of METTL1 inhibits methyltransferase activity by disrupting the catalytic centre. Cryo-EM structure, X-ray crystallography, biochemical methyltransferase assays, phosphomimetic mutagenesis, cellular complementation Nature High 36599985
2018 METTL1 and WDR4 form a complex that methylates a subset of 22 tRNAs at the N7-methylguanosine (m7G) position within a 'RAGGU' motif in the variable loop. Mettl1 or Wdr4 knockout in mouse ESCs causes increased ribosome occupancy at corresponding codons, indicating ribosome pausing and impaired mRNA translation. Knockout mESCs display defective self-renewal and neural differentiation. m7G-MeRIP-seq, TRAC-seq, ribosome profiling, genetic knockout in mESCs, differentiation assays Molecular cell High 29983320
2015 WDR4 is the human ortholog of yeast Trm82, an essential non-catalytic subunit of the Trm8/Trm82 holoenzyme that catalyzes m7G46 methylation of tRNA. A disease-causing missense mutation in WDR4 (and the corresponding yeast mutation) significantly reduces m7G46 methylation of specific tRNA species, causing a growth deficiency phenotype in yeast. Yeast genetics, tRNA methylation assay, autozygome/exome sequencing, functional complementation in yeast Genome biology High 26416026
2007 The yeast Trm8-Trm82 (ortholog of METTL1-WDR4) complex requires both the D-stem and T-stem structures of tRNA for efficient methyl-transfer to G46. Tertiary base pairs in the D-stem support but are not essential for induced fit of the G46 base into the catalytic pocket. In vitro methyl-transfer activity assay using truncated and mutant tRNA transcripts FEBS letters High 17382321
2007 Active yeast Trm8-Trm82 heterodimer (ortholog of METTL1-WDR4) is only produced when both subunit mRNAs are co-translated; mixing individually translated Trm8 and Trm82 proteins does not yield an active complex. This indicates that subunit association is translationally controlled. The complex catalyzes SAM-dependent methyl transfer to N7 of G46 in tRNA. Wheat germ cell-free translation system, in vitro reconstitution, kinetic enzymatic assay, 2D-TLC, aniline cleavage Journal of biotechnology High 18164779
2016 Drosophila Wuho (WDR4 ortholog) interacts with Flap Endonuclease 1 (FEN1). WH co-localizes with FEN1 and PCNA at sites of nascent DNA synthesis. WH modulates FEN1's endonucleolytic activities in a substrate-dependent manner: it stimulates FEN1's flap endonuclease activity but inhibits its gap endonuclease activity, thereby protecting replication fork integrity. Knockdown in Drosophila, mouse, and human cells causes DNA damage with strand breaks and apoptosis via ATM/Chk2/p53 signaling. Co-immunoprecipitation, fluorescence microscopy (colocalization), in vitro FEN1 endonuclease assay with WH, siRNA knockdown in multiple species, mouse knockout (embryonic lethal) PLoS biology High 26751069
2020 Drosophila Wuho (WDR4 ortholog) physically interacts with Mei-p26 (TRIM-NHL protein, ortholog of human TRIM32) and cooperates with it to maintain germline stem cell homeostasis. In GSCs, Wh and Mei-p26 promote BMP stemness signaling. In GSC progeny, they silence nanos translation, downregulate pro-differentiation microRNAs, and suppress ribosomal biogenesis via dMyc. Human WDR4 interacts with TRIM32 in human cells. Genetic epistasis (double mutants), Co-immunoprecipitation in Drosophila and human cells, translational reporter assays, stem cell functional assays Development (Cambridge, England) Medium 31941704
2023 WDR4 acts as a substrate adaptor of the CUL4 E3 ubiquitin ligase complex. WDR4-mediated ubiquitination of PTPN23 (an ESCRT component) leads to its proteasomal degradation, thereby suppressing lysosomal trafficking and degradation of EGFR and c-MET, sustaining their signaling in NSCLC. A competing peptide that blocks PTPN23 binding to WDR4 promotes EGFR/c-MET degradation. Unbiased ubiquitylome mass spectrometry, Co-IP, ubiquitination assay, lysosomal trafficking assay, competing peptide functional rescue, in vitro and in vivo tumor models Cell death & disease High 37821451
2023 WDR4 deficiency in granule neuron progenitors (GNPs) reduces cerebellar foliation and layer sizes, causing locomotion defects. Mechanistically, Wdr4 supports GNP proliferation by ubiquitination and proteasomal degradation of Arhgap17, thereby activating Rac1 to facilitate cell cycle progression. Disease-associated WDR4 variants cannot sustain this GNP cell cycle maintenance. Conditional knockout mouse model, cell cycle analysis, ubiquitination assay, Rac1 activity assay, rescue with disease variants Cell death & disease Medium 36681682
2024 WDR4 loss-of-function in human fibroblasts and zebrafish embryos impairs ciliogenesis. Mechanistically, WDR4 loss increases protein synthesis and upregulates proteasomal activity while reducing ubiquitin precursor pools. Restoring normal ubiquitin levels (by proteasome inhibition or ubiquitin supplementation) rescues ciliogenesis. Proteasome inhibition also ameliorates microcephaly phenotypes. Human fibroblast knockdown, zebrafish morpholino/mutant, patient-derived cells, proteasome activity assay, ubiquitin quantification, pharmacological rescue Cell death & disease Medium 39251572
2023 WDR4 promotes nuclear localization of DDX20 and acts as an adaptor to bridge DDX20 and the transcription factor EGR1, thereby inhibiting EGR1-promoted transcription of ARRB2, contributing to bladder cancer lymph node metastasis. Co-IP, nuclear fractionation, transcriptional reporter assay, gain/loss-of-function in bladder cancer cells and xenograft models Oncogenesis Medium 37783676
2021 METTL1/WDR4-mediated m7G tRNA modification enhances translation of mRNAs enriched for m7G tRNA-decoded codons. In lung cancer, knockdown of METTL1 reduces translation of mRNAs with higher frequencies of m7G-decoded codons, indicating that tRNA codon usage is a key mechanism by which the complex controls mRNA translation. m7G-MeRIP-seq, polysome profiling/translation assay, METTL1/WDR4 knockdown and overexpression, in vitro and in vivo tumor models, mutagenesis of METTL1 catalytic residues Molecular therapy High 34371184
2021 WDR4 promotes CCNB1 mRNA stability and translation in HCC by promoting binding of EIF2A to CCNB1 mRNA. WDR4 expression is transcriptionally activated by c-MYC. CCNB1 in turn promotes PI3K/AKT phosphorylation and reduces P53 protein by promoting its ubiquitination, defining a MYC/WDR4/CCNB1/PI3K/AKT/P53 signaling axis. RNA immunoprecipitation (RIP), Co-IP, mRNA stability assay, polysome profiling, ChIP, gain/loss-of-function, xenograft models Cell death & disease Medium 34244479
2025 Cytoplasmic WDR4 acts independently of m7G tRNA modification by directly interacting with eIF4E2, enhancing eIF4E-mediated selective translation of ABCA1, thereby promoting membrane cholesterol efflux and maintaining pro-tumoral macrophage polarization in HCC. Co-IP (WDR4-eIF4E2 interaction), polysome fractionation, cholesterol efflux assay, macrophage polarization assay, siRNA-CpG delivery in vivo, rescue with ABCA1 Nature cell biology Medium 41315768
2024 WDR4 modulates m7G modification at internal sites of tumor-promoting mRNAs by forming a WDR4-METTL1 protein complex (confirmed by Co-IP). WDR4 knockdown also downregulates METTL1 mRNA and protein expression, indirectly reducing complex formation. Co-IP, m7G-MeRIP-seq, dot blot, RNA-seq, knockdown/rescue experiments in HCC cells Cellular signalling Medium 38493882
2018 WDR4 deficiency in mouse embryonic fibroblasts (MEFs) induces γH2AX elevation, heterochromatin relaxation, p53 activation, caspase-mediated apoptosis, and p21-mediated G2/M cell cycle arrest, downstream of Wuho-FEN1 interaction. Tamoxifen-inducible Cre-mediated knockout in MEFs, western blotting, immunofluorescence for γH2AX, cell cycle analysis Cellular signalling Medium 29574139
2025 In Drosophila midgut, Wdr4 cooperates with Mettl1 to catalyze m7G modification of let-7 miRNA. Wdr4 or Mettl1 depletion reduces let-7 levels, aberrantly activates TOR-JNK-dMyc signaling, drives elevated ribosome biogenesis, ISC overproliferation and misdifferentiation. Human WDR4 and METTL1 (but not catalytic-dead METTL1) rescue these defects, establishing a conserved Wdr4/Mettl1-let-7-TOR-JNK axis. Drosophila genetic knockdown/overexpression, m7G miRNA modification assay, epistasis with TOR/JNK inhibitors, human WDR4/METTL1 rescue including catalytic-dead mutant EMBO reports Medium 41663759
2025 WDR4 promotes adipocyte browning by enhancing BMP8B mRNA translation through tRNA m7G modification. WDR4 overexpression in adipocytes reshapes the tRNA m7G methylome, increases translational activity of BMP8B and related genes, and induces mitophagy and UCP1 expression; BMP8B knockdown partially counteracts WDR4-mediated mitophagy. TRAC-seq, tRNA m7G methylome profiling, gain-of-function in 3T3-L1 and primary adipocytes, mitophagy assays, BMP8B knockdown rescue Adipocyte Medium 41292047
2025 METTL1/WDR4-mediated internal m7G methylation of SCLT1 mRNA stabilizes the transcript and promotes gefitinib resistance in NSCLC via NF-κB signaling activation. Wild-type METTL1 but not a catalytically inactive METTL1 mutant rescues SCLT1 mRNA stability upon knockdown. m7G MeRIP-seq, RNA-seq, mRNA stability assay, catalytic-dead METTL1 rescue, NF-κB reporter, in vitro and in vivo models Genomics, proteomics & bioinformatics Medium 40857569
2025 In colorectal cancer, WDR4 physically interacts with GSK3β (identified by Co-IP-LC/MS) and promotes GSK3β phosphorylation, thereby activating the β-catenin pathway to promote CRC proliferation and metastasis. Co-IP-LC/MS, western blotting for β-catenin pathway markers, gain/loss-of-function, xenograft models Biochemistry and cell biology Low 40009829
2006 Drosophila Wuho (WDR4 ortholog), a WD40-repeat protein with a bipartite nuclear localization signal, is required for spermatogenesis (arrests at elongating spermatid stage) and oogenesis (cystocyte division arrested at 4th mitotic cycle). Yeast homolog Trm82 is established as the non-catalytic subunit of a tRNA methylase. P-element mutagenesis, transgenic rescue, immunostaining, cytological analysis of germline Developmental biology Medium 16762337

Source papers

Stage 0 corpus · 41 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2018 Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation. Molecular cell 373 29983320
2021 METTL1/WDR4-mediated m7G tRNA modifications and m7G codon usage promote mRNA translation and lung cancer progression. Molecular therapy : the journal of the American Society of Gene Therapy 221 34371184
2021 MYC-targeted WDR4 promotes proliferation, metastasis, and sorafenib resistance by inducing CCNB1 translation in hepatocellular carcinoma. Cell death & disease 190 34244479
2022 Aberrant translation regulated by METTL1/WDR4-mediated tRNA N7-methylguanosine modification drives head and neck squamous cell carcinoma progression. Cancer communications (London, England) 164 35179319
2015 Mutation in WDR4 impairs tRNA m(7)G46 methylation and causes a distinct form of microcephalic primordial dwarfism. Genome biology 156 26416026
2023 Structures and mechanisms of tRNA methylation by METTL1-WDR4. Nature 92 36599982
2023 Structural basis of regulated m7G tRNA modification by METTL1-WDR4. Nature 88 36599985
2018 Mutations in WDR4 as a new cause of Galloway-Mowat syndrome. American journal of medical genetics. Part A 74 30079490
2022 Novel roles of METTL1/WDR4 in tumor via m7G methylation. Molecular therapy oncolytics 72 35784404
2000 Isolation and characterization of a human chromosome 21q22.3 gene (WDR4) and its mouse homologue that code for a WD-repeat protein. Genomics 52 10950928
2017 Further delineation of the phenotype caused by biallelic variants in the WDR4 gene. Clinical genetics 48 28617965
2023 METTL1/WDR4-mediated tRNA m7G modification and mRNA translation control promote oncogenesis and doxorubicin resistance. Oncogene 45 37185458
2007 RNA recognition mechanism of eukaryote tRNA (m7G46) methyltransferase (Trm8-Trm82 complex). FEBS letters 30 17382321
2024 RNA modification gene WDR4 facilitates tumor progression and immunotherapy resistance in breast cancer. Journal of advanced research 28 38960276
2023 WDR4/TRIM28 is a novel molecular target linked to lenvatinib resistance that helps retain the stem characteristics in hepatocellular carcinomas. Cancer letters 25 37279851
2016 Wuho Is a New Member in Maintaining Genome Stability through its Interaction with Flap Endonuclease 1. PLoS biology 25 26751069
2006 A new Drosophila gene wh (wuho) with WD40 repeats is essential for spermatogenesis and has maximal expression in hub cells. Developmental biology 21 16762337
2018 Speech and language delay in a patient with WDR4 mutations. European journal of medical genetics 20 29597095
2021 Aberrant expression of WDR4 affects the clinical significance of cancer immunity in pan-cancer. Aging 19 34282052
2023 PTPN23 ubiquitination by WDR4 suppresses EGFR and c-MET degradation to define a lung cancer therapeutic target. Cell death & disease 17 37821451
2020 WD40 protein Wuho controls germline homeostasis via TRIM-NHL tumor suppressor Mei-p26 in Drosophila. Development (Cambridge, England) 15 31941704
2024 WDR4 promotes HCC pathogenesis through N7-methylguanosine by regulating and interacting with METTL1. Cellular signalling 13 38493882
2007 Production of yeast tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex) in a wheat germ cell-free translation system. Journal of biotechnology 13 18164779
2022 WDR4 gene polymorphisms increase hepatoblastoma susceptibility in girls. Journal of Cancer 10 36186903
2023 Wdr4 promotes cerebellar development and locomotion through Arhgap17-mediated Rac1 activation. Cell death & disease 9 36681682
2023 WDR4 gene polymorphisms and Wilms tumor susceptibility in Chinese children: A five-center case-control study. Journal of Cancer 8 37283791
2025 Functions of METTL1/WDR4 and QKI as m7G modification - related enzymes in digestive diseases. Frontiers in pharmacology 5 39850560
2023 WDR4 promotes the progression and lymphatic metastasis of bladder cancer via transcriptional down-regulation of ARRB2. Oncogenesis 5 37783676
2021 Genetic Association of the Functional WDR4 Gene in Male Fertility. Journal of personalized medicine 5 34442404
2018 Wuho/WDR4 deficiency inhibits cell proliferation and induces apoptosis via DNA damage in mouse embryonic fibroblasts. Cellular signalling 3 29574139
2025 Upregulation of WDR4 mediated by RBFOX2 promotes laryngeal cancer progression through the WDR4/m7G/lncRNA ZFAS1/RBFOX2 axis. Naunyn-Schmiedeberg's archives of pharmacology 2 39774908
2025 WDR4 drives tumour-associated macrophage reprogramming and tumour progression via selective translation and membrane cholesterol efflux. Nature cell biology 2 41315768
2025 The P300/ETV4-WDR4 axis promotes colorectal cancer progression via m7G-mediated SPP1 mRNA stabilization and autophagy suppression. Cellular signalling 1 40681009
2025 METTL1-WDR4 promotes the migration and proliferation of gastric cancer through N7-methylguanosine. Cellular oncology (Dordrecht, Netherlands) 1 40764449
2025 METTL1/WDR4-mediated m7G Hypermethylation of SCLT1 mRNA Promotes Gefitinib Resistance in NSCLC. Genomics, proteomics & bioinformatics 1 40857569
2025 Mechanistic Insights Into METTL1/WDR4-Mediated m7G Modification in Prostate Cancer Progression and Its Potential as a Therapeutic Target. Journal of biochemical and molecular toxicology 1 41098012
2024 Cilia defects upon loss of WDR4 are linked to proteasomal hyperactivity and ubiquitin shortage. Cell death & disease 1 39251572
2026 Wdr4 regulates ribosome biogenesis and intestinal homeostasis via let-7. EMBO reports 0 41663759
2025 WDR4 promotes colorectal cancer progression by activating the GSK3β/β-catenin pathway. Biochemistry and cell biology = Biochimie et biologie cellulaire 0 40009829
2025 WDR4 promotes glioma progression by regulating cell proliferation and cell cycle via the PI3K/Akt-CDK1/2 signaling pathway. Neoplasma 0 41235531
2025 WDR4-mediate tRNA m7G modification to promote mitophagy and browning of white adipose tissue for ameliorating obesity in male mice. Adipocyte 0 41292047