Affinage

UBA7

Ubiquitin-like modifier-activating enzyme 7 · UniProt P41226

Length
1012 aa
Mass
111.7 kDa
Annotated
2026-06-10
100 papers in source corpus 20 papers cited in narrative 20 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

UBA7 (UBE1L) is the E1-activating enzyme that initiates conjugation of the interferon-induced ubiquitin-like modifier ISG15 to substrate proteins (ISGylation), a process functionally separable from ubiquitin signaling (PMID:37553340, PMID:14976209). Cryo-EM structures of UBA7 trapped with ISG15 adenylate and thioester intermediates and with the cognate E2 enzyme UBE2L6 (UbcH8) define how UBA7 adenylates ISG15 at its C-terminal diglycine/ubiquitin-like domain, forms a thioester intermediate, and transfers ISG15 to UBE2L6, revealing an architecture and specificity distinct from the ubiquitin cascade (PMID:37553340, PMID:38042859); the C-terminal diglycine motif of ISG15 is required for both physical association and conjugation (PMID:38042859, PMID:14976209). UBA7-deficient mice and cells fail to form ISG15 conjugates while retaining free ISG15, and this loss leaves JAK/STAT-driven IFN-α/β signaling intact, establishing UBA7 as dedicated specifically to conjugation (PMID:16382139). ISGylation contributes to antiviral defense, with UBA7 loss increasing susceptibility to influenza B (acting through radioresistant stromal cells) and Sindbis virus, while control of Chikungunya virus proceeds through a conjugation-independent immunomodulatory role of ISG15 (PMID:19004958, PMID:19073728, PMID:22028657). In cancer and differentiation contexts, UBA7 drives ISGylation of substrates including the PML domain of the PML/RARα oncoprotein and cyclin D1, targeting them for proteasomal degradation, and acts during retinoid-induced differentiation of acute promyelocytic leukemia cells (PMID:14976209, PMID:19074853, PMID:18413804). UBA7 additionally supports hematopoietic progenitor cell-cycle progression under interferon stress (PMID:20591702) and is itself targeted for degradation by the human cytomegalovirus protein pUL50 acting with the E3 ligase RNF170 (PMID:29743376).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1995 Medium

    Mapping the UBE1L locus and linking its silencing to epigenetic marks raised the question of whether loss of this gene is relevant to cancer.

    Evidence Genomic mapping, DNase I hypersensitivity, and methylation analysis in lung cancer cell lines

    PMID:7734949

    Open questions at the time
    • 5-azacytidine did not restore expression, implying additional regulatory blocks
    • no enzymatic function assigned at this stage
  2. 2002 Medium

    Established that UBE1L overexpression specifically degrades the PML/RARα oncoprotein and induces apoptosis, linking the enzyme to APL differentiation therapy.

    Evidence Retroviral overexpression with truncation/canonical-E1 controls and promoter assays in NB4 APL cells

    PMID:11891284

    Open questions at the time
    • overexpression-based; physiological relevance not yet tested
    • mechanism of degradation not resolved at the conjugation level
  3. 2004 High

    Identified UBE1L as the ISG15-specific E1 enzyme by demonstrating direct, diglycine-dependent association with ISG15 and a requirement for IFN/RA-induced ISGylation.

    Evidence Co-IP with ISG15 diglycine mutants and siRNA/shRNA knockdown in APL cells; affinity purification and thioester formation with bovine ortholog

    PMID:14976209 PMID:15385418

    Open questions at the time
    • E2 hand-off step not yet structurally defined
    • substrate repertoire of ISGylation unknown
  4. 2004 Medium

    Showed UBE1L catalytic activity is required to repress cyclin D1, extending its substrate range beyond PML/RARα.

    Evidence Microarray identification and transfection of wild-type versus mutant UBE1L in bronchial epithelial cells

    PMID:15520223

    Open questions at the time
    • mechanism of cyclin D1 repression not dissected here
    • single-lab transfection data
  5. 2006 High

    Genetic knockout established that UBA7 is required for all ISG15 conjugation but dispensable for free ISG15 and for JAK/STAT IFN signaling, cleanly separating conjugation from cytokine signaling.

    Evidence Ube1L-/- mice, VSV/LCMV infection, and epistasis with Ubp43 double-knockout

    PMID:16382139

    Open questions at the time
    • no antiviral phenotype against VSV/LCMV, leaving the in vivo role of conjugation open at this point
  6. 2008 Medium

    Defined the conjugation-dependent oncoprotein-degradation mechanism, mapping ISGylation to the PML domain and linking cyclin D1 loss to ISG15-conjugate formation and proteasomal turnover.

    Evidence Domain-construct transfection, co-IP of ISG15-substrate complexes, lysine-less cyclin D1 mutant, USP18 deconjugase antagonism, and proteasome inhibition

    PMID:18413804 PMID:19074853

    Open questions at the time
    • modified lysine residues not mapped
    • single-lab studies of substrate ISGylation
  7. 2008 High

    Demonstrated in vivo that UBA7-dependent ISGylation restricts specific viruses, with cell-type and conjugation-dependence established by chimera and mutant-virus approaches.

    Evidence Ube1L-/- mice with influenza B and Sindbis virus challenge, bone marrow chimeras, and an ISG15 R151A binding/transthiolation mutant

    PMID:19004958 PMID:19073728

    Open questions at the time
    • antiviral substrates of ISGylation not identified
    • mechanism of stromal-cell-restricted protection unresolved
  8. 2008 Medium

    Tested and excluded UBA7 as a tumor suppressor in K-ras-driven lung cancer, narrowing the contexts where its degradative activity is relevant.

    Evidence Ube1l-/- x K-rasLA2 mouse tumor progression and ISGylation analysis

    PMID:18571763

    Open questions at the time
    • does not exclude UBA7 relevance in other tumor genotypes
  9. 2010 Medium

    Revealed a cell-intrinsic role for UBA7 in hematopoietic progenitor cell-cycle progression under interferon stress.

    Evidence Competitive bone marrow transplantation of Ube1l-/- cells and cell cycle analysis

    PMID:20591702

    Open questions at the time
    • substrate driving G2/M progression not identified
    • single-lab study
  10. 2011 High

    Distinguished conjugation-dependent from conjugation-independent ISG15 antiviral functions by showing UBA7 is dispensable for Chikungunya control.

    Evidence Genetic epistasis with Ube1L-/- and ISG15-/- mouse lines and cytokine profiling

    PMID:22028657

    Open questions at the time
    • does not define which viruses depend on conjugation versus free ISG15 generally
  11. 2017 Medium

    Extended UBA7 antiviral function to neurotropic infection, showing it restricts rabies virus in vitro and in vivo.

    Evidence shRNA knockdown with viral titer and survival readouts in neuroblastoma, microglial cells, and mouse brain

    PMID:29056542

    Open questions at the time
    • substrates and mechanism of RABV restriction unknown
    • single method type
  12. 2018 Medium

    Identified a viral evasion mechanism in which HCMV pUL50, with RNF170, drives proteasomal degradation of UBA7 to suppress ISGylation.

    Evidence Reciprocal co-IP, domain-deletion mutants, and proteasome inhibitor rescue with pUL50 and RNF170 overexpression

    PMID:29743376

    Open questions at the time
    • single-lab; in vivo relevance during infection not established
  13. 2022 Medium

    Placed UBA7's ISG15 specificity in evolutionary context, showing it arose from UBA1 duplication and that ancestral Uba7 retained promiscuous ubiquitin-activating capacity later restricted to ISG15.

    Evidence Phylogenetic analysis and in vitro/in vivo ubiquitin activation assays in zebrafish

    PMID:35134494

    Open questions at the time
    • structural basis of specificity restriction addressed only later
    • single-lab evolutionary framing
  14. 2023 High

    Provided the structural mechanism of ISG15 activation and E1-to-E2 thioester transfer, explaining UBA7's exquisite selectivity for ISG15 and UBE2L6.

    Evidence Cryo-EM of UBA7-UBE2L6-ISG15 adenylate and thioester intermediates with biochemical mutant validation and viral effector tools

    PMID:37553340 PMID:38042859

    Open questions at the time
    • downstream E3 ligases and full substrate-loading mechanism not resolved in these structures

Open questions

Synthesis pass · forward-looking unresolved questions
  • The comprehensive set of physiological ISGylation substrates and the E3 ligases that complete conjugation downstream of UBA7-UBE2L6 remain to be systematically defined.
  • substrate-specific E3 ligases largely uncharacterized in this corpus
  • mechanism linking ISGylation to cell-cycle and antiviral effects not fully mapped

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016874 ligase activity 4 GO:0140096 catalytic activity, acting on a protein 3 GO:0140657 ATP-dependent activity 2
Pathway
R-HSA-168256 Immune System 3 R-HSA-392499 Metabolism of proteins 3
Partners
CCND1EGFRISG15PML/RARARNF170UBE2L6

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2023 Cryo-EM structures of human Uba7 (UBA7) in complex with UBE2L6 and ISG15 adenylate or ISG15 thioester intermediates reveal the molecular basis for ISG15 activation and E1-E2 thioester transfer. The structures show a unique overall architecture compared to the ubiquitin conjugation pathway, particularly with respect to the location of the ISG15 thioester intermediate, and illuminate UBA7's exquisite specificity for ISG15 and UBE2L6. Cryo-EM structure determination, biochemical assays, human cell-based experiments Nature communications High 37553340
2023 A 3.45 Å cryo-EM structure of a chemically trapped UBE1L (UBA7)-UBE2L6 complex bound to activated ISG15 reveals the first steps of ISG15 recognition and UBE2L6 recruitment by UBE1L. Biochemical characterization enabled design of ISG15 and UBE2L6 mutants with altered selectivity for the ISG15 versus ubiquitin conjugation pathways. The ISG15 C-terminal ubiquitin-like domain is required for the adenylation reaction, validated using viral effector proteins from SARS-CoV-2 and influenza B virus. Cryo-EM structure, biochemical mutant characterization, viral effector protein validation Nature communications High 38042859
2004 UBE1L (UBA7) physically associates with ISG15 in vivo, requiring the conserved C-terminal diglycine motif of ISG15. Knockdown of UBE1L with siRNA or shRNA inhibited IFN- and RA-induced ISG15 conjugation. UBE1L is required for ISG15 conjugation during retinoid-induced differentiation of acute promyelocytic leukemia (APL) cells. Co-immunoprecipitation, siRNA/shRNA knockdown, immunoblot The Journal of biological chemistry High 14976209
2006 Mice lacking UBE1L (Ube1L−/−) fail to form ISG15 conjugates but express free ISG15 normally. Ube1L−/− mice were fertile and showed normal antiviral responses against VSV and LCMV, indicating UBE1L and protein ISGylation are not critical for IFN-α/β signaling via JAK/STAT activation. Using Ube1L/Ubp43 double-deficient mice, increased IFN signaling in Ubp43-deficient mice was attributed to loss of UBP43, not increased ISGylation. Genetic knockout mouse model, viral infection assays, epistasis analysis with double-KO Molecular and cellular biology High 16382139
2002 UBE1L (UBA7) overexpression triggered PML/RARα degradation in NB4 APL cells and induced apoptosis; a truncated UBE1L or the canonical E1 enzyme did not cause this degradation. UBE1L is a direct retinoid target gene whose promoter is suppressed by PML/RARα, and its induction is tightly linked to PML/RARα degradation. UBE1L-induced apoptosis was specific to cells expressing PML/RARα. Retroviral overexpression, transfection, promoter assay, immunoblot Proceedings of the National Academy of Sciences of the United States of America Medium 11891284
2008 UBE1L promotes complex formation between ISG15 and cyclin D1, causing cyclin D1 protein (but not mRNA) degradation. UBE1L knockdown increased cyclin D1 protein; a lysine-less cyclin D1 mutant was resistant to UBE1L-mediated degradation. UBE1L retroviral transduction reduced cyclin D1 expression and clonal cell growth in bronchial epithelial and lung cancer cells. Co-immunoprecipitation, UBE1L retroviral transduction, siRNA knockdown, cycloheximide chase, immunoblot Molecular cancer therapeutics Medium 19074853
2008 UBE1L induces ISG15ylation of the PML domain of PML/RARα, targeting it for proteasomal degradation. RA-induced degradation targets the RARα domain via a distinct pathway, while UBE1L independently targets the PML domain. UBP43/USP18 (ISG15 deconjugase) opposed UBE1L- but not RA-dependent PML/RARα degradation; proteasomal inhibitor blocked both. Transfection of PML/RARα domain constructs, co-immunoprecipitation, proteasome inhibitor treatment, UBP43 deconjugase antagonism Molecular cancer therapeutics Medium 18413804
2008 Mice lacking UbE1L (the ISG15 E1 enzyme) fail to form ISG15 conjugates and display increased susceptibility to influenza B virus infection, including non-mouse-adapted strains. ISG15 controls influenza B virus infection through its action within radioresistant stromal cells, not bone marrow-derived cells, indicating stromal cell ISGylation is critical. Ube1L−/− mouse model, bone marrow transplantation/chimera experiments, viral infection assays Journal of virology High 19004958
2008 The ISG15 E1 enzyme UbE1L is important for control of Sindbis virus infection: UbE1L-deficient mice were highly susceptible to Sindbis virus. An ISG15 mutant (R151A) that ablates binding to UbE1L and transthiolation to UbcH8 could not protect IFN-αβR−/− mice from Sindbis virus lethality, supporting that protein conjugation (not free ISG15) mediates ISG15's antiviral effect against Sindbis virus. Ube1L−/− mouse model, double-subgenomic Sindbis virus expressing ISG15 mutants, kinetic binding assays, transfection Journal of virology High 19073728
2011 ISG15 controls Chikungunya virus (CHIKV) infection in neonatal mice independently of UbE1L-mediated conjugation: UbE1L−/− mice (unable to form ISG15 conjugates) showed no increase in lethality after CHIKV infection, whereas ISG15−/− mice were profoundly susceptible. ISG15 modulates proinflammatory cytokines/chemokines rather than controlling viral loads, indicating a non-conjugation, immunomodulatory role for ISG15 in CHIKV pathogenesis. UbE1L−/− and ISG15−/− mouse models, viral infection, cytokine/chemokine measurement PLoS pathogens High 22028657
2018 HCMV transmembrane protein pUL50 interacts with UBE1L (the ISG15 E1-activating enzyme) and causes its proteasomal degradation, thereby inhibiting ISGylation. This requires the C-terminal transmembrane domain of pUL50 for activity. RNF170, an ER-associated ubiquitin E3 ligase, interacts with pUL50 and promotes pUL50-mediated UBE1L ubiquitination and degradation. Co-immunoprecipitation, co-localization, proteasome inhibitor experiments, overexpression of UL50 and RNF170 Journal of virology Medium 29743376
2004 A 110-kDa bovine UBE1L protein was purified from endometrial cells by affinity with recombinant GST-ISG15, confirming a direct physical interaction between UBE1L and ISG15. Bovine UBE1L shares 83% cDNA identity with human UBE1L and forms a thioester bond with ISG15 to initiate conjugation. Affinity purification with GST-ISG15, mass spectrometry peptide identification, full-length cDNA sequencing Biology of reproduction Medium 15385418
2004 Microarray analyses identified UBE1L as a retinoid-regulated gene in human bronchial epithelial cells. Wild-type but not mutant UBE1L repressed cyclin D1 expression in a dose-dependent manner upon co-transfection, establishing a functional requirement for UBE1L's catalytic activity in cyclin D1 repression. Microarray, transfection with wild-type vs. mutant UBE1L, immunoblot Cancer research Medium 15520223
1995 The UBE1L gene has 22 exons spanning 8.4 kb at chromosome 3p21. Lack of UBE1L expression in lung cancer cell lines correlates with hypermethylation of a HhaI site in the first exon and decreased DNase I sensitivity of the promoter region, suggesting epigenetic silencing. Genomic mapping, anchored PCR, DNase I hypersensitivity assay, methylation analysis with 5-azacytidine treatment Gene expression Medium 7734949
2010 UBE1L-deficient (Ube1l−/−) hematopoietic cells show impaired repopulation potential after bone marrow transplantation (50% reduction at 3 weeks). Under conditions of high IFN and ISGylation (post-transplant), Ube1L deficiency causes G2/M cell cycle block in hematopoietic multipotential progenitors, indicating ISGylation promotes cell cycle progression during interferon-related stress. Bone marrow transplantation with Ube1l−/− cells, competitive transplantation, cell cycle analysis Blood cells, molecules & diseases Medium 20591702
2008 Ube1l deficiency did not alter lung cancer progression or overall survival in K-rasLA2 lung cancer mice, and protein ISGylation levels were largely unchanged during lung cancer progression in this model. This establishes a negative result: UBE1L is not a tumor suppressor gene in K-ras mutation-dependent lung cancer. Ube1l−/− crossed with K-rasLA2 mice, tumor progression and survival analysis, ISGylation western blots Lung cancer (Amsterdam, Netherlands) Medium 18571763
2017 shRNA knockdown of UBA7 increased rabies virus (RABV) titers in neuroblastoma (NA) and microglial (BV-2) cell lines and in mouse brains, and decreased mouse survival, establishing that UBA7 inhibits RABV replication in vitro and in vivo. shRNA knockdown, viral titer measurement, lentiviral-mediated knockdown in mouse brain, survival analysis Infection, genetics and evolution Medium 29056542
2013 UBE1L overexpression in human bronchial epithelial (Beas-2B) cells triggered EGFR membrane internalization, promoted complex formation between ISG15 and EGFR, and reduced EGFR protein at the post-translational level. UBE1L knockdown increased EGFR and downstream PI3K/AKT/NF-κB signaling. UBE1L overexpression, UBE1L knockdown, co-immunoprecipitation of ISG15-EGFR complex, immunoblot of downstream signaling The Journal of nutritional biochemistry Low 24445050
2022 Evolutionary analysis showed that UBA7 descends from gene duplication of UBA1, and ISG15 arose from UBB/UBC. Zebrafish Uba7 retains the ability to activate the ubiquitin cascade in vitro and in vivo (unlike tetrapod Uba7), indicating that ancestral Uba7 had promiscuous substrate specificity that became restricted to ISG15 in tetrapods. Phylogenetic analysis, in vitro ubiquitin activation assay, in vivo zebrafish assays Genomics Medium 35134494
2016 Ube1l−/− mouse embryonic fibroblasts (MEF) exhibit low viral resistance despite high STAT1/ISG expression compared to wild-type MEF. Ube1l−/− MEF populations contain two sub-populations with distinct basal STAT1 activity; this heterogeneity was perturbed by JAK2/FAK tyrosine kinase inhibitors (AG490, PF431396) but not by type I IFN neutralization, indicating UBE1L-dependent ISGylation regulates population-level basal immune signaling states through a tyrosine kinase-sensitive pathway. Ube1l−/− MEF, flow cytometry for STAT1 activity, tyrosine kinase inhibitors, IFN neutralization PloS one Low 27427993

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 slender rice, a constitutive gibberellin response mutant, is caused by a null mutation of the SLR1 gene, an ortholog of the height-regulating gene GAI/RGA/RHT/D8. The Plant cell 505 11340177
2011 ISG15 is critical in the control of Chikungunya virus infection independent of UbE1L mediated conjugation. PLoS pathogens 153 22028657
1997 B lymphocyte antigen D8/17: a peripheral marker for childhood-onset obsessive-compulsive disorder and Tourette's syndrome? The American journal of psychiatry 119 9054790
2008 Mice lacking the ISG15 E1 enzyme UbE1L demonstrate increased susceptibility to both mouse-adapted and non-mouse-adapted influenza B virus infection. Journal of virology 115 19004958
1988 Vaccinia virus gene D8 encodes a virion transmembrane protein. Journal of virology 111 3418784
2006 Ube1L and protein ISGylation are not essential for alpha/beta interferon signaling. Molecular and cellular biology 110 16382139
2002 UBE1L is a retinoid target that triggers PML/RARalpha degradation and apoptosis in acute promyelocytic leukemia. Proceedings of the National Academy of Sciences of the United States of America 96 11891284
2005 Simocyclinone D8, an inhibitor of DNA gyrase with a novel mode of action. Antimicrobial agents and chemotherapy 90 15728908
2007 Bacteroides sp. strain D8, the first cholesterol-reducing bacterium isolated from human feces. Applied and environmental microbiology 86 17616613
2009 A crystal structure of the bifunctional antibiotic simocyclinone D8, bound to DNA gyrase. Science (New York, N.Y.) 74 19965760
2008 ISG15 Arg151 and the ISG15-conjugating enzyme UbE1L are important for innate immune control of Sindbis virus. Journal of virology 73 19073728
2008 UBE1L causes lung cancer growth suppression by targeting cyclin D1. Molecular cancer therapeutics 73 19074853
1988 The dominant non-gibberellin-responding dwarf mutant (D8) of maize accumulates native gibberellins. Proceedings of the National Academy of Sciences of the United States of America 68 16594001
2006 Immunogenicity and protection efficacy of monovalent and polyvalent poxvirus vaccines that include the D8 antigen. Virology 64 16919703
2004 Involvement of UBE1L in ISG15 conjugation during retinoid-induced differentiation of acute promyelocytic leukemia. The Journal of biological chemistry 64 14976209
2014 In situ investigation of mammalian inorganic polyphosphate localization using novel selective fluorescent probes JC-D7 and JC-D8. ACS chemical biology 57 25007079
2015 Whole-Genome Sequencing of Measles Virus Genotypes H1 and D8 During Outbreaks of Infection Following the 2010 Olympic Winter Games Reveals Viral Transmission Routes. The Journal of infectious diseases 55 26153409
1999 B lymphocyte antigen D8/17 and repetitive behaviors in autism. The American journal of psychiatry 52 9989573
1989 HLA-DR/DQ antigens and reactivity to B cell alloantigen D8/17 in Indian patients with rheumatic heart disease. Circulation 48 2787710
1991 A molecular phylogeny of dinoflagellate protists (pyrrhophyta) inferred from the sequence of 24S rRNA divergent domains D1 and D8. Journal of molecular evolution 47 1901368
2012 SimReg1 is a master switch for biosynthesis and export of simocyclinone D8 and its precursors. AMB Express 45 22214346
2008 UBE1L represses PML/RAR{alpha} by targeting the PML domain for ISG15ylation. Molecular cancer therapeutics 42 18413804
2004 Microarray analyses uncover UBE1L as a candidate target gene for lung cancer chemoprevention. Cancer research 42 15520223
2012 Structural and biochemical characterization of the vaccinia virus envelope protein D8 and its recognition by the antibody LA5. Journal of virology 41 22623786
2013 Transcriptome analysis of cytoplasmic male sterility and restoration in CMS-D8 cotton. Plant cell reports 40 23743655
1995 Evaluation of an enterovirus group-specific anti-VP1 monoclonal antibody, 5-D8/1, in comparison with neutralization and PCR for rapid identification of enteroviruses in cell culture. Journal of clinical microbiology 38 7494045
2014 A new crystal structure of the bifunctional antibiotic simocyclinone D8 bound to DNA gyrase gives fresh insight into the mechanism of inhibition. Journal of molecular biology 37 24594357
2017 The antibacterial activity of E. coli bacteriophage lysin lysep3 is enhanced by fusing the Bacillus amyloliquefaciens bacteriophage endolysin binding domain D8 to the C-terminal region. Journal of microbiology (Seoul, Korea) 36 28124780
2009 In vivo and in vitro patterns of the activity of simocyclinone D8, an angucyclinone antibiotic from Streptomyces antibioticus. Antimicrobial agents and chemotherapy 36 19273673
2002 D8/17 expression on B lymphocytes in anorexia nervosa. The American journal of psychiatry 34 12153841
1993 Establishment and characterization of a new granulocyte-macrophage colony-stimulating factor-dependent and interleukin-3-dependent human acute myeloid leukemia cell line (GF-D8). Blood 33 8443395
2018 Transcriptome, cytological and biochemical analysis of cytoplasmic male sterility and maintainer line in CMS-D8 cotton. Plant molecular biology 32 30066309
2010 Proton-catalyzed hydrogenation of a d(8) Ir(I) complex yields a trans Ir(III) dihydride. Journal of the American Chemical Society 32 20232913
2022 Luminescence and Length Control in Nonchelated d8 -Metallosupramolecular Polymers through Metal-Metal Interactions. Angewandte Chemie (International ed. in English) 31 35749048
1999 Characterization of the human myeloid leukemia-derived cell line GF-D8 by multiplex fluorescence in situ hybridization, subtelomeric probes, and comparative genomic hybridization. Genes, chromosomes & cancer 31 10451701
2012 Cytotoxic hydrophilic iminophosphorane coordination compounds of d⁸ metals. Studies of their interactions with DNA and HSA. Journal of inorganic biochemistry 29 23063789
2017 Structure-function characterization of three human antibodies targeting the vaccinia virus adhesion molecule D8. The Journal of biological chemistry 28 29123031
2020 Controlled Supramolecular Polymerization of d8 Metal Complexes through Pathway Complexity and Seeded Growth. ChemPlusChem 26 32421254
2009 Anti-proliferative effects of simocyclinone D8 (SD8), a novel catalytic inhibitor of topoisomerase II. Investigational new drugs 26 19132295
2001 Progress toward analysis of D8/17 binding to B cells in children with obsessive compulsive disorder and/or chronic tic disorder. Journal of neuroimmunology 26 11694329
2018 Transmembrane Protein pUL50 of Human Cytomegalovirus Inhibits ISGylation by Downregulating UBE1L. Journal of virology 25 29743376
2001 Elevated D8/17 expression on B lymphocytes, a marker of rheumatic fever, measured with flow cytometry in tic disorder patients. The American journal of psychiatry 25 11282696
1998 A flow cytometric assay for D8/17 B cell marker in patients with Tourette's syndrome and obsessive compulsive disorder. Journal of immunological methods 25 9831399
1993 Diagnostic use of B-cell alloantigen D8/17 in rheumatic chorea. The Journal of pediatrics 25 8320631
2023 Cryo-EM structures of Uba7 reveal the molecular basis for ISG15 activation and E1-E2 thioester transfer. Nature communications 23 37553340
2004 Isolation and sequence of an interferon-tau-inducible, pregnancy- and bovine interferon-stimulated gene product 15 (ISG15)-specific, bovine ubiquitin-activating E1-like (UBE1L) enzyme. Biology of reproduction 23 15385418
2004 D8/17 and CD19 expression on lymphocytes of patients with acute rheumatic fever and Tourette's disorder. Clinical and diagnostic laboratory immunology 22 15013984
2014 Murine anti-vaccinia virus D8 antibodies target different epitopes and differ in their ability to block D8 binding to CS-E. PLoS pathogens 21 25474621
1992 A rapid test for the detection of a B-cell marker (D8/17) in rheumatic fever patients. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 21 1342610
2023 Insights into the ISG15 transfer cascade by the UBE1L activating enzyme. Nature communications 20 38042859
2002 Presence of the d8/17 B-cell marker in children with rheumatic fever in Israel. Clinical genetics 20 12030895
2013 Down-regulation of epidermal growth factor receptor by curcumin-induced UBE1L in human bronchial epithelial cells. The Journal of nutritional biochemistry 19 24445050
2008 First detection of Pectenotoxin-11 and confirmation of OA-D8 diol-ester in Dinophysis acuta from European waters by LC-MS/MS. Toxicon : official journal of the International Society on Toxinology 19 18926841
2002 Mechanism of silver-promoted ligand metathesis in square-planar complexes of d(8) Ions. Kinetics of formation and molecular structures of a trinuclear intermediate [(Me)(N-N)Pt(mu-Cl)Ag(mu-Cl)Pt(N-N)(Me)](+) and its dinuclear evolution product [(Me)(N-N)Pt(mu-Cl)Pt(N-N)(Me)](+) (N-N = ArN=C(Me)C(Me)=NAr, Ar = 2,6-(i-Pr)(2)C(6)H(3)). Inorganic chemistry 19 12005491
2013 Light-induced catalytic and cytotoxic properties of phosphorescent transition metal compounds with a d8 electronic configuration. Philosophical transactions. Series A, Mathematical, physical, and engineering sciences 18 23776290
2009 Mapping simocyclinone D8 interaction with DNA gyrase: evidence for a new binding site on GyrB. Antimicrobial agents and chemotherapy 17 19858260
1994 Tissue distribution of antigen(s) defined by monoclonal antibody D8/17 reacting with B lymphocytes of patients with rheumatic heart disease. Clinical immunology and immunopathology 17 8020192
1995 The genomic structure of the human UBE1L gene. Gene expression 16 7734949
2023 Maize WRKY28 interacts with the DELLA protein D8 to affect skotomorphogenesis and participates in the regulation of shade avoidance and plant architecture. Journal of experimental botany 15 36884355
2019 Up-regulation of interferon-stimulated gene 15 and its conjugation machinery, UbE1L and UbcH8 expression by tumor necrosis factor-α through p38 MAPK and JNK signaling pathways in human lung carcinoma. Molecular and cellular biochemistry 15 31428903
2013 Flavone-based analogues inspired by the natural product simocyclinone D8 as DNA gyrase inhibitors. Bioorganic & medicinal chemistry letters 15 24060488
1992 Frequency of D8/17 B lymphocyte alloantigen in north Indian patients with rheumatic heart disease. Immunology and cell biology 15 1639437
2020 Molecular Epidemiology of B3 and D8 Measles Viruses through Hemagglutinin Phylogenetic History. International journal of molecular sciences 14 32580384
2020 Generation and Transcriptome Profiling of Slr1-d7 and Slr1-d8 Mutant Lines with a New Semi-Dominant Dwarf Allele of SLR1 Using the CRISPR/Cas9 System in Rice. International journal of molecular sciences 14 32752068
2021 Physical mapping and InDel marker development for the restorer gene Rf2 in cytoplasmic male sterile CMS-D8 cotton. BMC genomics 13 33407111
2012 Inhibition of human topoisomerases I and II by simocyclinone D8. Journal of natural products 13 22867097
2011 Mass spectrometry reveals that the antibiotic simocyclinone D8 binds to DNA gyrase in a "bent-over" conformation: evidence of positive cooperativity in binding. Biochemistry 13 21338149
2010 Hematopoietic cells from Ube1L-deficient mice exhibit an impaired proliferation defect under the stress of bone marrow transplantation. Blood cells, molecules & diseases 13 20591702
2008 Deficiency of a potential 3p21.3 tumor suppressor gene UBE1L (UBA7) does not accelerate lung cancer development in K-rasLA2 mice. Lung cancer (Amsterdam, Netherlands) 13 18571763
2006 B-cell antigen D8/17 is a marker of rheumatic fever susceptibility in Aboriginal Australians and can be tested in remote settings. The Medical journal of Australia 13 16719750
2005 D8/17 monoclonal antibody: an unclear neuropsychiatric marker. Behavioural neurology 13 16082074
2017 Inhibition of rabies virus replication by interferon-stimulated gene 15 and its activating enzyme UBA7. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 12 29056542
2020 Aromatic Polyketides from a Symbiotic Strain Aspergillus fumigatus D and Characterization of Their Biosynthetic Gene D8.t287. Marine drugs 11 32575731
2016 Measles re-emergence in Northern Italy: Pathways of measles virus genotype D8, 2013-2014. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 11 27989661
2007 Presence of D8/17 B-cell marker in patients with poststreptococcal reactive arthritis. Rheumatology international 11 17221172
2018 Furosine Induced Apoptosis by the Regulation of STAT1/STAT2 and UBA7/UBE2L6 Genes in HepG2 Cells. International journal of molecular sciences 10 29857509
2019 LEKTI domains D6, D7 and D8+9 serve as substrates for transglutaminase 1: implications for targeted therapy of Netherton syndrome. The British journal of dermatology 9 30801672
2009 Characterisation of the constitutive over-expression of AJ18 in a novel rat stromal bone marrow cell line (D8-SBMC). Archives of oral biology 9 19473652
2009 Simocyclinone D8 turns on against Gram-negative bacteria in a clinical setting. Bioorganic & medicinal chemistry letters 9 20022496
2020 Development and utilization of an InDel marker linked to the fertility restorer genes of CMS-D8 and CMS-D2 in cotton. Molecular biology reports 8 31894465
2019 [13C6,D8]2-deoxyglucose phosphorylation by hexokinase shows selectivity for the β-anomer. Scientific reports 8 31873121
2013 Identification of mitochondrial DNA sequence variation and development of single nucleotide polymorphic markers for CMS-D8 in cotton. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik 8 23435734
2002 Establishment and characterization of transplantable tumor line (KB) and cell lines (KB-P and KB-D8) from a rat thymus-derived dendritic cell sarcoma. Virchows Archiv : an international journal of pathology 8 12070602
2021 Characterization of an In Vivo Neutralizing Anti-Vaccinia Virus D8 Single-Chain Fragment Variable (scFv) from a Human Anti-Vaccinia Virus-Specific Recombinant Library. Vaccines 7 34835240
2015 Mechanism of Dose-Dependent Regulation of UBE1L by Polyphenols in Human Bronchial Epithelial Cells. Journal of cellular biochemistry 7 25649257
2024 Role of Ubiquitin-conjugating enzyme E2 (UBE2) in two immune-mediated inflammatory skin diseases: a mendelian randomization analysis. Archives of dermatological research 6 38795139
2016 Towards measles elimination: Phylogenetic analysis of measles viruses in Turkey (2012-2013) and identification of genotype D8. Journal of medical virology 6 27089242
2016 Occurrence of measles genotype D8 during a 2014 outbreak in Banjarmasin, South Kalimantan, Indonesia. International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 6 27825950
2008 Does hybridization increase evolutionary rate? Data from the 28S-rDNA D8 domain in echinoderms. Journal of molecular evolution 6 18949506
2002 Immunophenotypical changes of neoplastic cells and tumor-associated macrophages in a rat dendritic cell sarcoma-derived transplantable tumor line (KB-D8). Virchows Archiv : an international journal of pathology 6 12596065
2022 Insights into the evolution of the ISG15 and UBA7 system. Genomics 5 35134494
1996 Red cell Na+/H+ exchange and B cell alloantigen 883 (D8/17) in patients with acute rheumatic fever and inactive rheumatic heart disease. Scandinavian journal of rheumatology 5 8614772
2022 Transcript Complexity and New Insights of Restorer Line in CMS-D8 Cotton Through Full-Length Transcriptomic Analysis. Frontiers in plant science 4 35800603
2022 Machine Learning Prediction of Adenovirus D8 Conjunctivitis Complications from Viral Whole-Genome Sequence. Ophthalmology science 4 36531578
2019 The first outbreak of measles virus caused by imported genotype D8 in Jiangsu province of China. The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases 4 30876879
2014 Molecular epidemiology of measles virus infection in Shanghai in 2000-2012: the first appearance of genotype D8. The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases 4 25281832
2011 Markers of susceptibility to acute rheumatic fever: the B-cell antigen D8/17 is not robust as a marker in South Africa. Cardiology in the young 4 21303578
2009 Crystallization and preliminary X-ray analysis of a complex formed between the antibiotic simocyclinone D8 and the DNA breakage-reunion domain of Escherichia coli DNA gyrase. Acta crystallographica. Section F, Structural biology and crystallization communications 4 19652356
2025 Identification of UBA7 expression downregulation in myelodysplastic neoplasm with SF3B1 mutations. Scientific reports 3 40158006
2016 AG490 and PF431396 Sensitive Tyrosine Kinase Control the Population Heterogeneity of Basal STAT1 Activity in Ube1l Deficient Cells. PloS one 3 27427993

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