Affinage

TEP1

Telomerase protein component 1 · UniProt Q99973

Length
2627 aa
Mass
290.5 kDa
Annotated
2026-06-10
100 papers in source corpus 20 papers cited in narrative 20 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

The TEP1 timeline resolves into two internally coherent but biologically distinct proteins linked by a shared gene symbol, reflecting a symbol collision across species and historical nomenclature. In mammals, TEP1/TLP1 is a dual-function RNA-binding protein: it co-purifies with telomerase and binds telomerase RNA, but it is dispensable for telomerase catalytic activity and telomere length maintenance in vivo (PMID:9118230, PMID:11027287, PMID:11029039), and it is independently a structural subunit of the vault ribonucleoprotein particle where it is required for stable association and recruitment of vault RNA (PMID:10551828, PMID:11149928). A single Tetrahymena p80-homology RNA-binding domain (residues 1–871) directly binds both telomerase RNA and vault RNA in competition, and is necessary and sufficient to target TEP1 to vaults (PMID:15701761). A second cluster of mammalian findings instead describes PTEN/MMAC1, a cytoplasmic protein tyrosine and inositol-phosphate phosphatase that antagonizes PI3K/Akt signaling, raises p27(KIP1), arrests cells in G1, and suppresses Ras-induced transformation (PMID:9187108, PMID:9860981, PMID:10698513); this PTEN-associated work uses the TEP1 alias and a yeast ortholog acting in the phosphatidylinositol pathway (PMID:11070083). In mosquitoes (Anopheles gambiae), TEP1 is an entirely separate complement C3-like thioester-containing protein of the innate immune system that circulates in the hemolymph, is secreted by the fat body, and after protease-dependent cleavage binds and kills Plasmodium parasites by lysis and melanization (PMID:15006349, PMID:28095489). Mosquito TEP1 is held in its inactive thioester-intact conformation by a TED-MG8 domain interface (PMID:17606907, PMID:23055931); cleavage drives TED-MG8 separation, which is prerequisite for binding the LRIM1/APL1C leucine-rich-repeat heterodimer through its coiled-coil domain, and this complex stabilizes the reactive cleaved form and delivers it to parasite surfaces (PMID:19286136, PMID:21533217, PMID:31237887). Upstream, a CLIPA2 clip-domain protease homolog restrains TEP1 convertase activity to limit TEP1 consumption (PMID:25012124), and TEP1 also promotes periostial hemocyte aggregation during bacterial infection (PMID:30690067). Because these clusters describe unrelated proteins, the molecular_activity and pathway slots below are populated separately and the narrative should not be read as one protein.

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 1997 High

    Two independent identifications established the symbol's ambiguity: one assigned TEP1 to a cytoplasmic tyrosine phosphatase (PTEN/MMAC1), the other to a co-purifying protein subunit of telomerase.

    Evidence In vitro phosphatase assay and immunofluorescence (PTEN); anti-TLP1 immunoprecipitation of telomerase activity and co-purification with pulse-chase PTM analysis (rat telomerase)

    PMID:9118230 PMID:9187108

    Open questions at the time
    • The two 1997 findings describe unrelated proteins sharing a symbol
    • Whether TEP1 contributes catalytically to telomerase was not addressed
  2. 1998 High

    PTEN/MMAC1/TEP1 was placed in a defined growth-suppressive signaling cascade, answering how it controls proliferation.

    Evidence Cell cycle analysis, Western blot, kinase assay and pharmacological inhibition in glioblastoma cells

    PMID:9860981

    Open questions at the time
    • Concerns the PTEN protein, not the telomerase/vault TEP1
    • Direct phosphatase substrate in cells inferred via epistasis
  3. 1999 High

    TEP1 was shown to be a bona fide vault particle component that binds vault RNAs sequence-specifically, separating its RNA-binding role from telomerase catalysis.

    Evidence Vault purification, TRAP activity assay, yeast three-hybrid RNA-binding assay

    PMID:10551828

    Open questions at the time
    • Functional consequence of TEP1–vault RNA binding not yet established
    • Structural basis of recruitment unresolved
  4. 2000 High

    A knockout and reconstitution work resolved that TEP1 is dispensable for telomerase catalysis in vivo and that TEP1/RNA binding is uncoupled from polymerization.

    Evidence mTep1−/− mice with TRAP and telomere FISH across seven generations; hTERT deletion mutagenesis with in vitro reconstitution

    PMID:11027287 PMID:11029039

    Open questions at the time
    • Why telomerase retains TEP1 if catalytically dispensable is unexplained
    • TEP1's non-telomerase RNA role pointed toward vaults but not yet mechanistically dissected
  5. 2000 High

    Structure-function and ortholog studies tied the PTEN-associated TEP1 alias to lipid-phosphatase activity and an evolutionarily conserved phosphatidylinositol pathway role.

    Evidence NIH3T3 transformation/soft-agar assays with phosphatase and Akt readouts; S. cerevisiae deletion genetics with wortmannin/lithium resistance and sporulation phenotype

    PMID:10698513 PMID:11070083

    Open questions at the time
    • Yeast ortholog evidence is Medium-confidence
    • These findings concern PTEN, distinct from telomerase/vault TEP1
  6. 2001 High

    The vault role was made mechanistic: TEP1 is required for stable association of vault RNA with the particle and for normal cap structure.

    Evidence Cryo-EM 3D reconstruction, vault purification and RNA quantification in mTep1−/− mice

    PMID:11149928

    Open questions at the time
    • Functional consequence of vault RNA loss for cells unresolved
    • Whether reduced cap density alters vault cargo handling unknown
  7. 2004 High

    A wholly distinct mosquito TEP1 was established as a complement-like effector that binds and kills malaria parasites, founding the innate-immunity branch.

    Evidence dsRNA knockdown with parasite counting and melanization assays in Anopheles gambiae

    PMID:15006349

    Open questions at the time
    • Activation mechanism and required cofactors not yet defined
    • Mechanism of parasite killing (lysis vs melanization) not separated
  8. 2005 High

    The mammalian TEP1 RNA-binding determinant was mapped to a single p80-homology domain sufficient for both telomerase RNA and vault RNA binding and for vault targeting.

    Evidence Yeast three-hybrid, EMSA direct binding, deletion mutagenesis, vault targeting assay

    PMID:15701761

    Open questions at the time
    • Structural basis of RNA specificity not solved
    • How a single domain partitions between telomerase and vaults in cells unresolved
  9. 2007 High

    Crystallography defined how mosquito TEP1 maintains an inactive thioester-intact conformation and how allelic variation maps to thioester protection.

    Evidence X-ray crystallography of TEP1r isoform

    PMID:17606907

    Open questions at the time
    • Conformational transition upon activation not captured
    • In vivo trigger for thioester exposure not defined here
  10. 2009 High

    The LRIM1/APL1 LRR proteins were shown to stabilize circulating TEP1 and enable its binding to parasites, explaining how the effector is kept available in hemolymph.

    Evidence RNAi silencing with hemolymph immunoblot and parasite binding assay

    PMID:19286136

    Open questions at the time
    • Molecular interface of stabilization not yet defined
    • Whether stabilization acts on full-length or cleaved TEP1 unresolved
  11. 2011 High

    The LRIM1/APL1C coiled-coil domain was identified as the docking site for mature cleaved TEP1, generalizing the interaction to multiple TEP proteins.

    Evidence Allele-mutant expression, co-immunoprecipitation, disulfide-bond mutagenesis

    PMID:21533217

    Open questions at the time
    • Stoichiometry of the in vivo complex not determined
    • Functional consequence of binding other TEPs not characterized
  12. 2012 High

    Full-length structure plus biochemistry linked allelic susceptibility to thioester hydrolysis and showed LRIM1/APL1C stabilizes the reactive cleaved form.

    Evidence X-ray crystallography of TEP1*S1, thioester hydrolysis assay, complex formation

    PMID:23055931

    Open questions at the time
    • Dynamics of MG1-MG6 flexibility in solution not resolved
    • How stabilization translates to parasite-surface deposition not shown here
  13. 2014 Medium

    A regulatory layer was added: CLIPA2 restrains TEP1 convertase activity to limit TEP1 consumption during infection.

    Evidence RNAi knockdown with hemolymph TEP1 cleavage immunoblot and infection scoring

    PMID:25012124

    Open questions at the time
    • Convertase identity inferred indirectly, not isolated
    • Direct CLIPA2–convertase interaction not demonstrated
  14. 2017 Medium

    The source and secretion of mosquito TEP1 were localized, establishing the fat body as the main expression site and confirming hemolymph circulation of the active cleaved form.

    Evidence GFP reporter transgene, transgenic rescue, hemolymph immunoblot, parasite counting

    PMID:28095489

    Open questions at the time
    • Single-lab reporter localization
    • Allele dominance behavior not mechanistically explained
  15. 2019 High

    The activation logic was completed for mosquito TEP1 and extended to a new effector role: TED-MG8 separation upon cleavage is prerequisite for LRIM1/APL1C binding, and TEP1 also drives periostial hemocyte aggregation in bacterial infection.

    Evidence Engineered disulfide mutagenesis with Co-IP (activation); RNAi with intravital imaging and bacterial/melanin quantification (periostial role)

    PMID:30690067 PMID:31237887

    Open questions at the time
    • How TED-MG8 separation is structurally coupled to coiled-coil binding not solved
    • Mechanism by which TEP1 recruits hemocytes to ostia unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how the mammalian TEP1 RNA-binding subunit functions biologically given its dispensability for telomerase, and what cellular role vault RNA recruitment serves.
  • No cellular phenotype tied to TEP1 vault RNA recruitment
  • No structural model of the TEP1 RNA-binding domain–RNA complex

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 3 GO:0005198 structural molecule activity 2 GO:0016787 hydrolase activity 2
Localization
GO:0005576 extracellular region 1 GO:0005829 cytosol 1
Pathway
R-HSA-168256 Immune System 3 R-HSA-162582 Signal Transduction 2
Partners
APL1CHTERTLRIM1TEP3
Complex memberships
LRIM1/APL1C heterodimer complextelomerasevault ribonucleoprotein particle

Evidence

Reading pass · 20 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 TEP1 (identical to PTEN/MMAC1) possesses intrinsic protein tyrosine phosphatase activity, demonstrated by in vitro phosphatase assays using the PTP signature motif. The protein is localized to the cytoplasm by immunofluorescence and shares homology with tensin and auxilin. In vitro phosphatase assay, immunofluorescence Cancer research High 9187108
1997 TLP1/TEP1 encodes a protein component of rat telomerase; anti-TLP1 antibody immunoprecipitated telomerase activity, and the protein was co-purified with telomerase through extensive purification steps. The p240 form is post-translationally modified to the p230 form in vivo, and p230 is the dominant form in telomerase-positive cells. Immunoprecipitation, co-purification, pulse-chase experiment Cell High 9118230
1998 PTEN/MMAC1/TEP1 suppresses glioblastoma cell growth by blocking G1 cell cycle progression, correlating with increased p27(KIP1) and decreased G1 CDK activities. PTEN expression inhibits Akt/PKB, and this effect on p27 and the cell cycle can be mimicked by PI3-kinase inhibitor LY294002, placing PTEN upstream of PI3K/Akt/p27. Cell cycle analysis, Western blotting, kinase assay, pharmacological inhibition Proceedings of the National Academy of Sciences of the United States of America High 9860981
1999 TEP1 is a component of the vault ribonucleoprotein particle (the 240 kDa vault protein); vaults purified with TEP1 present have no detectable telomerase activity. Using a yeast three-hybrid assay, several human vault RNAs interact with TEP1 in a sequence-specific manner. Partial cDNA identification, vault purification, telomerase activity assay (TRAP), yeast three-hybrid assay The Journal of biological chemistry High 10551828
2000 mTep1-deficient mice are viable with normal telomerase activity, normal telomere length across seven generations, and no reactivation of telomerase in normally telomerase-negative tissues. TEP1 is thus dispensable for telomerase function in vivo. TEP1 also specifically binds vault RNA (vRNA), establishing it as an RNA-binding protein not restricted to the telomerase complex. Knockout mouse (mTep1−/−), TRAP assay, telomere length analysis (FISH), RNA binding Molecular and cellular biology High 11027287
2001 In mTep1−/− mice, vault particles appear structurally intact but show reduced cap density by cryo-EM 3D reconstruction. Critically, vault RNA is completely absent from purified vaults and is destabilized (reduced levels) in the absence of TEP1, establishing TEP1 as required for stable association of vault RNA with the vault particle. Cryo-electron microscopy, 3D reconstruction, vault purification, RNA quantification The Journal of cell biology High 11149928
2000 Specific hTERT deletions that retain interaction with telomerase RNA and TEP1 are completely inactive for telomerase polymerization in vitro and in vivo, demonstrating that TEP1 binding and RNA binding can be functionally uncoupled from catalysis. The amino-terminus of hTERT is required for primer elongation but not for RNA or TEP1 binding. In vitro reconstitution of telomerase activity, deletion mutagenesis, in vivo assay Molecular biology of the cell High 11029039
2000 PTEN/TEP1 inhibits H-Ras–induced transformation and anchorage-independent growth in NIH3T3 cells by suppressing the PI3K-dependent (but not MAPK) signaling cascade. C-terminal truncation mutants show that anti-transformation activity correlates with ability to dephosphorylate inositol-(1,3,4,5)-tetrakisphosphate in vitro and to suppress Akt kinase activity in cells. NIH3T3 transformation assay, soft-agar growth, in vitro phosphatase assay, Akt kinase activity measurement, deletion mutagenesis Oncogene High 10698513
2004 Anopheles gambiae TEP1 (complement-like protein) binds to and mediates killing of Plasmodium berghei midgut stages. dsRNA knockdown of TEP1 completely abolishes melanotic refractoriness in a refractory mosquito strain and increases parasite numbers in susceptible mosquitoes. dsRNA knockdown (RNAi), parasite counting, melanization assay Cell High 15006349
2005 The Tetrahymena p80 homology region (amino acids 1–871) of TEP1 is necessary and sufficient for interaction with both telomerase RNA and vault RNA in yeast three-hybrid assays, and for targeting TEP1 to the vault particle. Recombinant TEP1 RNA-binding domain directly binds RNA (EMSA), and vault RNA competes with telomerase RNA for this domain. Yeast three-hybrid assay, electrophoretic mobility shift assay (EMSA), deletion mutagenesis, vault targeting assay Nucleic acids research High 15701761
2007 Crystal structure of Anopheles gambiae TEP1 isoform TEP1r reveals an overall fold resembling complement factor C3 but with repositioned domains that stabilize the inactive (thioester-intact) conformation in the absence of the anaphylotoxin domain. Structural differences between TEP1r and TEP1s alleles map to the TED-MG8 interface protecting the thioester bond. X-ray crystallography Proceedings of the National Academy of Sciences of the United States of America High 17606907
2009 LRIM1 and APL1 leucine-rich repeat proteins are required for binding of TEP1 to Plasmodium parasites. RNAi silencing of LRIM1 or APL1 causes deposition of TEP1 on mosquito tissues, depleting circulating TEP1 and preventing its binding to parasites. LRIM1 and APL1 stabilize each other and stabilize circulating TEP1. RNAi knockdown, hemolymph immunoblot, parasite binding assay Cell host & microbe High 19286136
2011 The coiled-coil domain of the LRIM1/APL1C complex is the binding site for mature (cleaved) TEP1. Key cysteine residues form the intermolecular disulfide bond of the LRIM1/APL1C heterodimer. The complex also interacts with mature forms of TEP3 and two other TEP proteins via the same coiled-coil domain. Cell culture expression of allele mutants, co-immunoprecipitation, disulfide-bond mutagenesis PLoS pathogens High 21533217
2012 Crystal structure of full-length TEP1*S1 allele shows flexibility in MG1-MG6 domains relative to TEP1*R1. Amino acid differences localize to the TED-MG8 interface protecting the thioester; cleaved TEP1*S1 is significantly more susceptible to thioester hydrolysis than TEP1*R1. The LRIM1/APL1C complex stabilizes the thioester in cleaved TEP1*S1. X-ray crystallography, thioester hydrolysis assay, biochemical complex formation PLoS pathogens High 23055931
2014 CLIPA2 (clip domain serine protease homolog) negatively regulates TEP1 consumption during systemic infections by localizing to microbial surfaces in a TEP1-dependent manner and inhibiting the putative TEP1 convertase that cleaves full-length TEP1-F into active TEP1cut. CLIPA2 silencing triggers exacerbated TEP1-mediated immunity. RNAi knockdown, hemolymph immunoblot (TEP1 cleavage assay), infection phenotype scoring Journal of innate immunity Medium 25012124
2019 Cleaved TEP1 forms a soluble complex with the LRIM1/APL1C heterodimer through the coiled-coil domain of LRIM1/APL1C. Complex formation requires a conformational change in the TED-MG8 domain interface of TEP1 induced by cleavage; disulfide-stabilized TEP1 (TED-MG8 locked) does not interact with LRIM1/APL1C, establishing that TED-MG8 separation is prerequisite for complex formation. Engineered disulfide mutagenesis, co-immunoprecipitation, biochemical stability assay PloS one High 31237887
2000 The S. cerevisiae TEP1 ortholog (YNL128w) functions in the phosphatidylinositol pathway: tep1 diploids are resistant to the PI3-kinase inhibitor wortmannin and to lithium. A common human PTEN tumor mutation introduced at the analogous yeast position produces a non-functional Tep1p. TEP1 is required for normal dityrosine deposition on spore walls during sporulation. Yeast deletion genetics, drug resistance assay, sporulation phenotype analysis, site-directed mutagenesis Proceedings of the National Academy of Sciences of the United States of America Medium 11070083
2006 PARP-1 knockdown by siRNA in HeLa cells reduces poly(ADP-ribosyl)ation of hTERT and decreases TEP1/TP1 protein expression, correlating with reduced telomerase activity. This establishes PARP-1 as a regulator of TEP1 expression/function in the telomerase complex. siRNA knockdown, TRAP assay, Western blot, immunoprecipitation of poly(ADP-ribosyl)ated proteins Mutation research Medium 17141279
2019 TEP1, TEP3, and TEP4 in Anopheles gambiae are positive regulators of periostial hemocyte aggregation at the heart ostia during bacterial infection. RNAi knockdown of TEP1 reduced periostial hemocyte numbers, bacterial accumulation, and melanin deposition at periostial regions, without affecting non-periostial sessile hemocytes. RNAi knockdown, intravital imaging, bacterial burden quantification, melanization scoring Insect biochemistry and molecular biology Medium 30690067
2017 TEP1 is secreted and circulates in the mosquito hemolymph; its activated cleaved form binds and eliminates malaria parasites. The fat body is the main site of TEP1 expression by GFP reporter assay. Transgenic TEP1r rescues loss-of-function TEP1 mutations but does not increase parasite resistance in the presence of a susceptible wild-type allele. GFP reporter transgene, transgenic rescue, hemolymph immunoblot, parasite counting PLoS pathogens Medium 28095489

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1997 TEP1, encoded by a candidate tumor suppressor locus, is a novel protein tyrosine phosphatase regulated by transforming growth factor beta. Cancer research 792 9187108
2004 Complement-like protein TEP1 is a determinant of vectorial capacity in the malaria vector Anopheles gambiae. Cell 488 15006349
1998 PTEN/MMAC1/TEP1 suppresses the tumorigenicity and induces G1 cell cycle arrest in human glioblastoma cells. Proceedings of the National Academy of Sciences of the United States of America 425 9860981
1997 TLP1: a gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family. Cell 369 9118230
2009 Two mosquito LRR proteins function as complement control factors in the TEP1-mediated killing of Plasmodium. Cell host & microbe 184 19286136
1998 Inactivation of the PTEN/MMAC1/TEP1 gene in human lung cancers. Genes, chromosomes & cancer 136 9598803
1999 Vaults and telomerase share a common subunit, TEP1. The Journal of biological chemistry 134 10551828
1999 PTEN/MMAC1/TEP1 in signal transduction and tumorigenesis. European journal of biochemistry 118 10469123
1998 PTEN/MMAC1/TEP1 involvement in primary prostate cancers. Oncogene 112 9671408
1996 Chromatin reorganization in rat spermatids during the disappearance of testis-specific histone, H1t, and the appearance of transition proteins TP1 and TP2. Biology of reproduction 94 8722637
1988 A cytochemical study of the transcriptional and translational regulation of nuclear transition protein 1 (TP1), a major chromosomal protein of mammalian spermatids. The Journal of cell biology 93 3372585
1980 Pleiotropic mutations rendering Escherichia coli K-12 resistant to bacteriophage TP1. Journal of bacteriology 91 6997269
2001 The Telomerase/vault-associated protein TEP1 is required for vault RNA stability and its association with the vault particle. The Journal of cell biology 82 11149928
2011 Structure-function analysis of the Anopheles gambiae LRIM1/APL1C complex and its interaction with complement C3-like protein TEP1. PLoS pathogens 76 21533217
2000 Polymerization defects within human telomerase are distinct from telomerase RNA and TEP1 binding. Molecular biology of the cell 74 11029039
1994 Identification of a functional cyclic adenosine 3',5'-monophosphate response element in the 5'-flanking region of the gene for transition protein 1 (TP1), a basic chromosomal protein of mammalian spermatids. Biology of reproduction 74 7888512
2007 Structural basis for conserved complement factor-like function in the antimalarial protein TEP1. Proceedings of the National Academy of Sciences of the United States of America 72 17606907
2000 Telomerase-associated protein TEP1 is not essential for telomerase activity or telomere length maintenance in vivo. Molecular and cellular biology 72 11027287
2001 PTEN/MMAC1/TEP1 mutations in human primary renal-cell carcinomas and renal carcinoma cell lines. International journal of cancer 69 11146448
1989 Nucleotide sequence of the Stp-1 gene coding for rat spermatid nuclear transition protein 1 (TP1): homology with protamine P1 and assignment of the mouse Stp-1 gene to chromosome 1. Gene 66 2524424
2000 Inhibition of H-Ras transformation by the PTEN/MMAC1/TEP1 tumor suppressor gene. Oncogene 58 10698513
2001 Stimulation of DNA repair by the spermatidal TP1 protein. Molecular reproduction and development 54 11241781
2001 Suppression of tumorigenicity and metastasis in B16F10 cells by PTEN/MMAC1/TEP1 gene. Cancer letters 54 11595133
2014 A serine protease homolog negatively regulates TEP1 consumption in systemic infections of the malaria vector Anopheles gambiae. Journal of innate immunity 51 25012124
2017 Transgenic Expression of the Anti-parasitic Factor TEP1 in the Malaria Mosquito Anopheles gambiae. PLoS pathogens 48 28095489
2002 TLP-1 is an asymmetric cell fate determinant that responds to Wnt signals and controls male tail tip morphogenesis in C. elegans. Development (Cambridge, England) 46 11880358
2000 Telomerase activity is down regulated via decreases in hTERT mRNA but not TEP1 mRNA or hTERC during the differentiation of leukemic cells. Anticancer research 46 10810353
1990 Interaction of spermatid-specific protein TP2 with nucleic acids, in vitro. A comparative study with TP1. The Journal of biological chemistry 46 2250010
1994 Crucial sequences within the Epstein-Barr virus TP1 promoter for EBNA2-mediated transactivation and interaction of EBNA2 with its responsive element. Journal of virology 41 7933133
1990 Identification of Epstein-Barr virus terminal protein 1 (TP1) in extracts of four lymphoid cell lines, expression in insect cells, and detection of antibodies in human sera. Journal of virology 41 2159542
2008 The evolution of TEP1, an exceptionally polymorphic immunity gene in Anopheles gambiae. BMC evolutionary biology 40 18840262
1991 Retrotransposon-like nature of Tp1 elements: implications for the organisation of highly repetitive, hypermethylated DNA in the genome of Physarum polycephalum. Nucleic acids research 38 1707520
2016 Variation in susceptibility of African Plasmodium falciparum malaria parasites to TEP1 mediated killing in Anopheles gambiae mosquitoes. Scientific reports 37 26861587
1998 The genetic basis of Cowden's syndrome: three novel mutations in PTEN/MMAC1/TEP1. Human genetics 35 9600246
2002 p53 and PTEN/MMAC1/TEP1 gene therapy of human prostate PC-3 carcinoma xenograft, using transferrin-facilitated lipofection gene delivery strategy. Human gene therapy 34 11936974
2002 Downregulation of PTEN/MMAC/TEP1 expression in human prostate cancer cell line DU145 by growth stimuli. Molecular and cellular biochemistry 34 12190124
2000 Alternative splicing of the human PTEN/MMAC1/TEP1 gene. Biochimica et biophysica acta 34 11121587
2004 Multiple roles of CLAN (caspase-associated recruitment domain, leucine-rich repeat, and NAIP CIIA HET-E, and TP1-containing protein) in the mammalian innate immune response. Journal of immunology (Baltimore, Md. : 1950) 33 15528373
2013 Genetic variants in telomerase reverse transcriptase (TERT) and telomerase-associated protein 1 (TEP1) and the risk of male infertility. Gene 32 24269974
2012 Molecular basis for genetic resistance of Anopheles gambiae to Plasmodium: structural analysis of TEP1 susceptible and resistant alleles. PLoS pathogens 31 23055931
2005 The p80 homology region of TEP1 is sufficient for its association with the telomerase and vault RNAs, and the vault particle. Nucleic acids research 31 15701761
2016 The crystal structure of the tandem-PAS sensing domain of Campylobacter jejuni chemoreceptor Tlp1 suggests indirect mechanism of ligand recognition. Journal of structural biology 30 26923153
2006 Purification, characterization, and gene cloning of 4-hydroxybenzoate decarboxylase of Enterobacter cloacae P240. Archives of microbiology 30 16758158
1987 Isolation of a cDNA clone for transition protein 1 (TP1), a major chromosomal protein of mammalian spermatids. Gene 30 2820847
1991 Differential expression of Epstein Barr viral transcripts for two proteins (TP1 and LMP) in lymphocyte and epithelial cells. Nucleic acids research 29 1710355
2017 miR-380-5p-mediated repression of TEP1 and TSPYL5 interferes with telomerase activity and favours the emergence of an "ALT-like" phenotype in diffuse malignant peritoneal mesothelioma cells. Journal of hematology & oncology 28 28716051
1994 The immunodominant Eimeria acervulina sporozoite antigen previously described as p160/p240 is a 19-kilodalton antigen present in several Eimeria species. Molecular and biochemical parasitology 28 8183325
2020 hsa_circ_0004018 suppresses the progression of liver fibrosis through regulating the hsa-miR-660-3p/TEP1 axis. Aging 25 32584784
2017 Late-phase immune responses limiting oocyst survival are independent of TEP1 function yet display strain specific differences in Anopheles gambiae. Parasites & vectors 24 28764765
2015 Mapping of Post-translational Modifications of Transition Proteins, TP1 and TP2, and Identification of Protein Arginine Methyltransferase 4 and Lysine Methyltransferase 7 as Methyltransferase for TP2. The Journal of biological chemistry 23 25818198
2000 TEP1, the yeast homolog of the human tumor suppressor gene PTEN/MMAC1/TEP1, is linked to the phosphatidylinositol pathway and plays a role in the developmental process of sporulation. Proceedings of the National Academy of Sciences of the United States of America 23 11070083
2019 Complement-like proteins TEP1, TEP3 and TEP4 are positive regulators of periostial hemocyte aggregation in the mosquito Anopheles gambiae. Insect biochemistry and molecular biology 21 30690067
2005 In vitro and in planta interaction evidence between Nicotiana tabacum thaumatin-like protein 1 (TLP1) and cucumber mosaic virus proteins. Plant molecular biology 19 16307370
2015 Adaptive Potential of Hybridization among Malaria Vectors: Introgression at the Immune Locus TEP1 between Anopheles coluzzii and A. gambiae in 'Far-West' Africa. PloS one 16 26047479
2006 Inhibition of telomerase activity by reduction of poly(ADP-ribosyl)ation of TERT and TEP1/TP1 expression in HeLa cells with knocked down poly(ADP-ribose) polymerase-1 (PARP-1) gene. Mutation research 16 17141279
2013 Ability of TEP1 in intestinal flora to modulate natural resistance of Anopheles dirus. Experimental parasitology 15 23648664
2010 Association of sporadic breast cancer with PTEN/MMAC1/TEP1 promoter hypermethylation. Medical oncology (Northwood, London, England) 15 20237868
1997 Comparative gene mapping of the human and mouse TEP1 genes, which encode one protein component of telomerases. Genomics 15 9403057
2023 Ginsentide TP1 Protects Hypoxia-Induced Dysfunction and ER Stress-Linked Apoptosis. Cells 14 37408235
2016 Evolution of the NET (NocA, Nlz, Elbow, TLP-1) protein family in metazoans: insights from expression data and phylogenetic analysis. Scientific reports 14 27929068
1984 Biological and biochemical characterization of a new isolate of feline sarcoma virus: Theilen-Pedersen (TP1-FeSV). Virology 14 6208683
2009 Spatiotemporal organization of AT- and GC-rich DNA and their association with transition proteins TP1 and TP2 in rat condensing spermatids. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 13 19506090
1995 Ultrastructural immunolocalisation of histones (H2B, H3, H4), transition protein (TP1) and protamine in rabbit spermatids and spermatozoa nuclei. Relation to condensation of the chromatin. Reproduction, nutrition, development 12 8526985
1992 Applications of an automated apparatus for two-dimensional electrophoresis, Model TEP-1, for microsequence analyses of proteins. Electrophoresis 11 1459096
2020 Tep1 Regulates Yki Activity in Neural Stem Cells in Drosophila Glioma Model. Frontiers in cell and developmental biology 9 32457905
2015 Genetic variants in the TEP1 gene are associated with prostate cancer risk and recurrence. Prostate cancer and prostatic diseases 9 26238235
2023 The dCache Domain of the Chemoreceptor Tlp1 in Campylobacter jejuni Binds and Triggers Chemotaxis toward Formate. mBio 8 37052512
2020 Prediction, isolation, overexpression and antifungal activity analysis of Medicago truncatula var. truncatula putative thaumatin like proteins (TLP-1, -2, -3, -4 and -5). Turkish journal of biology = Turk biyoloji dergisi 7 32922125
2019 Anopheles gambiae TEP1 forms a complex with the coiled-coil domain of LRIM1/APL1C following a conformational change in the thioester domain. PloS one 7 31237887
2018 Deletion of ptn1, a PTEN/TEP1 Orthologue, in Ustilago maydis Reduces Pathogenicity and Teliospore Development. Journal of fungi (Basel, Switzerland) 7 30577430
2017 Quantitative real-time PCR analysis of Anopheles dirus TEP1 and NOS during Plasmodium berghei infection, using three reference genes. PeerJ 6 28761783
2000 [Loss and inactivation of PTEN/MMAC1/TEP1 gene in lung cancer]. Zhonghua bing li xue za zhi = Chinese journal of pathology 6 11866895
1999 Co-localization of HP1 and TP1 transcripts in human spermatids by double electron microscopy in situ hybridization. International journal of andrology 6 10194639
1989 The TP1 isolate of feline sarcoma virus encodes a fgr-related oncogene lacking gamma actin sequences. Oncogene 6 2539576
2025 CircMAN1A2 Levels Determine GBM Susceptibility to TMZ in a Pathway Involving TEP1- and KEAP1-Mediated NRF2 Degradation Leading to Ferroptosis. CNS neuroscience & therapeutics 5 40583858
2022 Sequence Diversity of Tp1 and Tp2 Antigens and Population Genetic Analysis of Theileria parva in Unvaccinated Cattle in Zambia's Chongwe and Chisamba Districts. Pathogens (Basel, Switzerland) 5 35215058
2022 Insights into the equilibrium structure and translocation mechanism of TP1, a spontaneous membrane-translocating peptide. Scientific reports 5 36400938
2021 hsa_circ_0005721 triggers proliferation, migration and invasion of osteosarcoma by upregulating the linear transcript TEP1. Journal of B.U.ON. : official journal of the Balkan Union of Oncology 5 34565023
1999 Complement-mediated lysis of cultured osteosarcoma cell lines using chimeric mouse/human TP-1 IgG1 and IgG3 antibodies. Cancer immunology, immunotherapy : CII 5 10501855
1997 Activation of human Kupffer cells by thymostimulin (TP-1) to produce cytotoxicity against human hepatocellular cancer. Annals of surgical oncology 5 9084852
2022 Vectorial capacity and TEP1 genotypes of Anopheles gambiae sensu lato mosquitoes on the Kenyan coast. Parasites & vectors 4 36457004
2016 Association of PINX1 but not TEP1 Polymorphisms with Progression to Hepatocellular Carcinoma in Thai Patients with Chronic Hepatitis B Virus Infection. Asian Pacific journal of cancer prevention : APJCP 4 27221889
2014 Molecular cloning of precursors for TEP-1 and TEP-2: The GGNG peptide-related peptides of a prosobranch gastropod, Thais clavigera. Peptides 4 25451871
2012 Crystal structure of a monomeric thiolase-like protein type 1 (TLP1) from Mycobacterium smegmatis. PloS one 4 22844533
2023 The Influence of TEP1 and TERC Genetic Variants on the Susceptibility to Multiple Sclerosis. Journal of clinical medicine 3 37762804
2019 A new role for vault RNA-TEP1 complexes in mRNA production in trypanosomes. The Journal of biological chemistry 3 31653772
2010 Plasmodium yoelii: correlation of TEP1 with mosquito melanization induced by nitroquine. Experimental parasitology 3 20599985
2000 [Mutation analysis of the tumor suppressor gene PTEN/MMAC1/TEP1 in human hepatocellular carcinoma]. Shi yan sheng wu xue bao 2 12549040
1983 Effect of treatment with thymustimulin (Tp-1) on T and B cells in lymphoproliferative disorders. Blut 2 6603885
1982 Enhancement by TP-1, a thymic extract, of pokeweed mitogen-induced differentiation of B cells from normal subjects but not of those from patients with systemic lupus erythematosus. Thymus 2 6977207
2025 Comparative immunological roles of TEP1 in Anopheles gambiae and Biomphalaria glabrata: implications for malaria and schistosomiasis control. Frontiers in immunology 1 41169373
1988 In vitro effect of TP-1 (a calf thymic extract) on suppressor T-cell function of patients with autoimmune chronic active hepatitis. International journal of immunopharmacology 1 2974021
2025 M6A Methylase METTL3-mediated downregulation of TEP1 mRNA stability attenuates PDGF-BB-induced HASMC remodeling and inflammation via macrophage polarization. BMC pediatrics 0 40604488
2024 Genotype distribution and allele frequency of thioester-containing protein 1(Tep1) and its effect on development of Plasmodium oocyst in populations of Anopheles arabiensis in Ethiopia. PloS one 0 39383173
2022 Evaluation of genotoxicity of SUNACTIVE Zn-P240 in vitro and in vivo. Toxicological research 0 36277369
2021 Evaluation of 28-day repeated oral dose toxicity of SUNACTIVE Zn-P240 in rats. Regulatory toxicology and pharmacology : RTP 0 34242707
2011 [Function of TEP1 gene during Plasmodium yoelii infection in Anopheles dirus]. Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 0 21823319
2009 [Correlation of Anopheles TEP1 gene with melanization induced by nitroquine]. Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 0 20066988
2003 [Correlation between the PTEN/MMAC1/TEP1 expression and cell proliferation and apoptosis in human renal cell carcinoma (RCC)]. Ai zheng = Aizheng = Chinese journal of cancer 0 12948410
2002 [The expression of human telomerase-associated protein hTERT and TEP1 in cord blood stem/progenitor cells and its significance]. Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi 0 12133453

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