Affinage

TAPBP

Tapasin · UniProt O15533

Round 2 corrected
Length
448 aa
Mass
47.6 kDa
Annotated
2026-04-28
130 papers in source corpus 12 papers cited in narrative 12 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

Tapasin (TAPBP) is a transmembrane glycoprotein of the immunoglobulin superfamily that functions as the central adaptor and peptide editor within the MHC class I peptide-loading complex (PLC) in the endoplasmic reticulum. Its C-terminal region binds the TAP peptide transporter while its N-terminal domain stabilizes MHC class I–β2m dimers, bridging the two into a functional loading complex and optimizing the peptide repertoire for high-affinity ligands (PMID:8769474, PMID:10382748, PMID:11970875). Tapasin forms a stable disulfide-linked heterodimer with the oxidoreductase ERp57, and this conjugate—not tapasin alone—constitutes the minimal functional unit sufficient for MHC class I recruitment, peptide loading, and peptide editing, as demonstrated by cell-free reconstitution and confirmed by a 2.6 Å crystal structure that mapped the ERp57-contact and MHC class I-binding surfaces (PMID:17603487, PMID:19119025). TAPBP is required for normal MHC class I surface expression and immune recognition, as shown by rescue of tapasin-null cells and TALEN-mediated knockout in human embryonic stem cells (PMID:9271576, PMID:27068360).

Mechanistic history

Synthesis pass · year-by-year structured walk · 10 steps
  1. 1996 High

    Discovery of tapasin as a novel ER-resident glycoprotein that physically bridges MHC class I–β2m–calreticulin complexes to the TAP transporter answered how peptide-loading substrates are coupled to the peptide supply machinery.

    Evidence Immunoprecipitation and biochemical fractionation in tapasin-negative .220 cells and wild-type cells

    PMID:8769474

    Open questions at the time
    • Molecular cloning not yet achieved
    • Stoichiometry of tapasin per TAP unknown
    • Mechanism by which tapasin promotes peptide loading unresolved
  2. 1997 High

    Cloning of the TAPBP gene established tapasin as an MHC-linked, IgSF transmembrane protein with an ER retention signal, and functional rescue in .220 cells demonstrated that tapasin expression is both necessary and sufficient to restore class I–TAP association, surface expression, and CTL recognition.

    Evidence Molecular cloning, transfection rescue of .220 cells, co-IP, CTL killing assay, peptide binding assays in microsomes

    PMID:9238042 PMID:9271576

    Open questions at the time
    • Direct peptide-editing activity not yet demonstrated
    • Structure of tapasin unknown
    • Binding partners beyond TAP and class I not defined
  3. 1998 High

    Identification of ERp57 (ER-60) as a component of tapasin-containing MHC class I assembly complexes revealed that the PLC includes an oxidoreductase, raising the question of whether ERp57 plays a catalytic or structural role.

    Evidence MALDI mass spectrometry peptide mapping and co-IP in multiple cell lines

    PMID:9545232

    Open questions at the time
    • Nature of ERp57-tapasin interaction (covalent vs. noncovalent) unknown
    • Functional contribution of ERp57 to peptide loading unresolved
  4. 1999 High

    Deletion mutagenesis mapped tapasin's functional domains, showing that the N-terminal 50 residues stabilize MHC class I in the PLC while the C-terminal region mediates TAP binding, establishing tapasin as a modular adaptor.

    Evidence Systematic tapasin truncation mutants, co-IP, Michaelis-Menten kinetic analysis of peptide transport

    PMID:10382748

    Open questions at the time
    • Atomic-level contacts with MHC class I and TAP not known
    • Whether tapasin directly contacts peptide remains unclear
  5. 2002 High

    Analysis of natural HLA-B polymorphisms and reconstituted loading assays demonstrated that tapasin actively edits the MHC class I peptide repertoire to favor high-affinity peptides, establishing peptide editing as a discrete function beyond simple adaptor activity.

    Evidence Cell-free peptide-loading reconstitution, peptide elution/comparison, HLA-B*4402 vs. B*4405 polymorphism analysis

    PMID:11970875

    Open questions at the time
    • Molecular mechanism of peptide editing (how tapasin destabilizes suboptimal peptides) unknown
    • Whether editing mechanism differs across HLA alleles not tested systematically
  6. 2005 High

    Demonstration that tapasin and ERp57 form a stable disulfide-linked heterodimer in cells and in vitro, with noncovalent interactions suppressing ERp57 reductase activity, resolved whether ERp57 acts catalytically or structurally in the PLC—it serves primarily a structural scaffolding role.

    Evidence In vitro reconstitution with recombinant proteins, reductase activity assays, IFN-γ induction, co-IP

    PMID:16193070

    Open questions at the time
    • Whether residual ERp57 catalytic activity contributes under physiological conditions not excluded
    • Structure of the heterodimer not yet available
  7. 2007 High

    Cell-free reconstitution proved that the tapasin–ERp57 conjugate, not tapasin alone, is the minimal functional unit for MHC class I recruitment, peptide loading, and peptide editing, resolving why tapasin alone was insufficient in earlier assays.

    Evidence Purified recombinant tapasin–ERp57 conjugate in cell-free peptide binding and competition assays

    PMID:17603487

    Open questions at the time
    • Whether additional PLC components (calreticulin, calnexin) modulate editing efficiency in this system not tested
    • Kinetic parameters of editing not determined
  8. 2009 High

    The 2.6 Å crystal structure of the tapasin–ERp57 heterodimer revealed the structural basis for heterodimer stability and, combined with mutagenesis, identified a conserved surface on tapasin that is critical for MHC class I interaction and peptide editing.

    Evidence X-ray crystallography, site-directed mutagenesis, functional peptide loading assays

    PMID:19119025

    Open questions at the time
    • No co-crystal with MHC class I or TAP to show actual PLC assembly
    • Conformational changes during peptide editing not captured
  9. 2016 Medium

    TALEN knockout of TAPBP in human ESCs confirmed its requirement for MHC class I surface expression in a non-transformed human cell context, extending earlier mutant cell line findings to a clinically relevant system.

    Evidence TALEN-mediated gene disruption in hESCs, flow cytometry, pluripotency assays

    PMID:27068360

    Open questions at the time
    • Immune evasion phenotype not tested in vivo
    • Whether alternative peptide-loading pathways compensate long-term not assessed
  10. 2024 Medium

    A 3′ UTR SNP (rs1059288) was shown to increase TAPBP mRNA m6A modification and expression via METTL14/YTHDF2, linking TAPBP overexpression to cervical cancer cell proliferation, migration, and JAK/STAT pathway activation—an unexpected oncogenic role distinct from its canonical immune function.

    Evidence MeRIP-seq, TCGA integration, case-control SNP study, siRNA knockdown, overexpression, pathway analysis

    PMID:38992170

    Open questions at the time
    • Oncogenic mechanism independent of MHC class I loading not established
    • Single-lab finding without in vivo immune contextualization
    • Whether JAK/STAT activation is direct or secondary to altered antigen presentation not resolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • A co-crystal structure of tapasin (or the tapasin–ERp57 heterodimer) in complex with MHC class I and/or TAP is needed to reveal the atomic mechanism of peptide editing—how tapasin destabilizes suboptimal peptides and promotes exchange.
  • No PLC holoCcomplex structure at atomic resolution
  • Allele-specific differences in tapasin dependence not structurally explained
  • In vivo significance of TAPBP overexpression in cancer not clarified

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 3 GO:0044183 protein folding chaperone 2 GO:0098772 molecular function regulator activity 2
Localization
GO:0005783 endoplasmic reticulum 5
Pathway
R-HSA-168256 Immune System 4 R-HSA-392499 Metabolism of proteins 4
Partners
B2MCALRCANXPDIA3TAP1TAP2
Complex memberships
MHC class I peptide-loading complex (PLC)Tapasin–ERp57 heterodimer

Evidence

Reading pass · 12 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1996 Tapasin (TAPBP gene product) was identified as a novel 48 kDa glycoprotein that bridges MHC class I-β2m-calreticulin complexes to TAP in the endoplasmic reticulum, functioning as an adaptor required for class I-TAP association and peptide loading. Immunoprecipitation, biochemical fractionation, and functional assays in mutant cell lines lacking tapasin (cell line .220) Immunity High 8769474
1997 Molecular cloning of tapasin revealed it to be a transmembrane glycoprotein encoded by an MHC-linked gene, a member of the immunoglobulin superfamily with a cytoplasmic ER retention signal; up to four MHC class I-tapasin complexes bind each TAP molecule, and tapasin expression in tapasin-negative mutant cells (line .220) restores class I-TAP association, normal class I surface expression, and CTL recognition of virus-infected cells. Molecular cloning, expression in mutant cell line, immunoprecipitation, CTL killing assay Science High 9271576
1997 TAP-A (tapasin) was cloned as a 448-residue type I membrane glycoprotein with a double-lysine ER retention motif that forms a stoichiometric complex with TAP1/2; class I heavy chain and β2m co-precipitate with this complex, and TAP-A binds peptides in an ATP-dependent manner (unlike TAP1/2), suggesting a direct role in peptide loading onto MHC class I dimers. cDNA cloning, immunoprecipitation with anti-TAP1 and anti-TAP-A antisera, pulse-chase, cross-linkable peptide binding assay in microsomes Proceedings of the National Academy of Sciences of the United States of America High 9238042
1998 The thiol-dependent reductase ER-60 (ERp57) was identified as a component of MHC class I assembly complexes including tapasin-TAP; ER-60 is found in both early calnexin-heavy chain complexes and in late complexes containing tapasin, TAP, calreticulin, and calnexin, suggesting a role in disulfide bond formation during class I folding. MALDI mass spectrometry peptide mapping, co-immunoprecipitation, identification in tapasin-containing complexes The EMBO journal High 9545232
1999 The N-terminal 50 residues of tapasin are the key element that converts weak individual interactions between MHC class I molecules and TAP into a stable loading complex; binding to TAP is mediated by the C-terminal region, and this interaction increases TAP levels without affecting intrinsic translocation rate. Deletion mutant analysis of tapasin, Michaelis-Menten kinetic analysis of peptide transport, co-immunoprecipitation European journal of immunology High 10382748
2002 Tapasin is required for optimization of the MHC class I peptide cargo over time, both quantitatively and qualitatively improving peptide repertoire; a single natural polymorphism at position 116 in HLA-B*4402 (116D→Y in B*4405) enables tapasin-independent loading, and in the presence of tapasin B*4405 acquires a less optimal repertoire than B*4402, revealing tapasin's peptide-editing function. Reconstituted peptide-loading assays, mutant allele analysis, peptide elution and comparison of peptide repertoires Immunity High 11970875
2002 A related gene TAPBP-R was identified at chromosome 12p13.3, encoding TAPASIN-R, an IgSF member with structural motifs similar to tapasin but with marked differences in the V domain, transmembrane, and cytoplasmic regions; TAPASIN-R localizes predominantly to the ER but also shows some cell-surface expression, and lacks an obvious ER retention signal. Genomic identification, expression cloning using mouse ortholog, subcellular localization by biotinylation and fractionation European journal of immunology Medium 11920573
2005 Tapasin forms a stable disulfide-linked heterodimer with ERp57 within the MHC class I peptide-loading complex; the vast majority of cellular tapasin is disulfide-linked to ERp57, and tapasin upregulation by IFN-γ sequesters most ERp57 into the PLC. The heterodimer forms spontaneously in vitro, and noncovalent interactions inhibit ERp57's thioredoxin reductase activity, maintaining the interaction and suggesting a structural rather than catalytic role for ERp57 in the PLC. In vitro reconstitution with recombinant proteins, co-immunoprecipitation, IFN-γ induction experiments, reductase activity assays The EMBO journal High 16193070
2007 The tapasin-ERp57 conjugate (but not recombinant tapasin alone) is the functional unit of the peptide-loading complex: using a cell-free system, the tapasin-ERp57 heterodimer recruits MHC class I molecules, facilitates peptide binding, and edits the peptide repertoire to maximize affinity, while recombinant tapasin alone is insufficient for these functions. Cell-free reconstitution system with purified recombinant tapasin-ERp57 conjugate, peptide binding and competition assays Nature immunology High 17603487
2009 The 2.6 Å crystal structure of the tapasin-ERp57 core of the peptide-loading complex revealed that tapasin interacts with both catalytic domains of ERp57, accounting for heterodimer stability; mutational analysis identified a conserved surface on tapasin required for MHC class I interaction and critical for peptide loading and editing functions; a molecular model of the assembled PLC was generated. X-ray crystallography (2.6 Å), site-directed mutagenesis, functional peptide loading assays Immunity High 19119025
2016 Disruption of TAPBP in human embryonic stem cells (hESCs) using TALEN technology resulted in deficient MHC class I expression at the cell surface and reduced immunogenicity compared to wild-type cells, while maintaining normal pluripotency, karyotype, and differentiation ability, establishing that TAPBP is required for MHC class I surface expression in hESCs. TALEN-mediated gene disruption in hESCs, flow cytometry for MHC class I, pluripotency assays Bioscience, biotechnology, and biochemistry Medium 27068360
2024 A risk SNP rs1059288 (A>G) in the 3' UTR of TAPBP increases m6A modification of TAPBP mRNA, facilitated by methyltransferase METTL14 and reader YTHDF2, leading to increased TAPBP expression; TAPBP overexpression promotes cervical cancer cell growth, migration, tumor formation, and chemoresistance, and knockdown of TAPBP inhibits the JAK/STAT/MICB signaling pathway and upregulates immune genes including ISG15, IRF3, PTPN6, and HLA-A. MeRIP-seq, TCGA/RNA-seq data integration, case-control SNP study, siRNA knockdown, overexpression experiments, pathway analysis Archives of toxicology Medium 38992170

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2012 Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell 1718 22658674
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
1989 TEN versus TPN following major abdominal trauma--reduced septic morbidity. The Journal of trauma 609 2501509
1996 Roles for calreticulin and a novel glycoprotein, tapasin, in the interaction of MHC class I molecules with TAP. Immunity 555 8769474
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
1998 CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system. Annual review of immunology 417 9597125
1997 A critical role for tapasin in the assembly and function of multimeric MHC class I-TAP complexes. Science (New York, N.Y.) 411 9271576
2005 Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome research 409 16344560
1995 The CD19/CR2/TAPA-1 complex of B lymphocytes: linking natural to acquired immunity. Annual review of immunology 396 7542009
1990 TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins. Molecular and cellular biology 356 1695320
2012 Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins. Nature 319 22810586
2002 Optimization of the MHC class I peptide cargo is dependent on tapasin. Immunity 285 11970875
2003 The DNA sequence and analysis of human chromosome 6. Nature 242 14574404
2009 Insights into MHC class I peptide loading from the structure of the tapasin-ERp57 thiol oxidoreductase heterodimer. Immunity 240 19119025
1998 Regulation of endothelial cell motility by complexes of tetraspan molecules CD81/TAPA-1 and CD151/PETA-3 with alpha3 beta1 integrin localized at endothelial lateral junctions. The Journal of cell biology 227 9566977
2007 hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. Genomics 222 17207965
2007 Selective loading of high-affinity peptides onto major histocompatibility complex class I molecules by the tapasin-ERp57 heterodimer. Nature immunology 204 17603487
1996 Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin alpha 4 beta 1 (CD49d/CD29). Journal of immunology (Baltimore, Md. : 1950) 200 8757325
1993 Functional dissection of the CD21/CD19/TAPA-1/Leu-13 complex of B lymphocytes. The Journal of experimental medicine 196 7690834
1998 ER-60, a chaperone with thiol-dependent reductase activity involved in MHC class I assembly. The EMBO journal 193 9545232
2006 ERp57 is essential for efficient folding of glycoproteins sharing common structural domains. The EMBO journal 171 17170699
2020 Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2. Nature communications 167 32572027
1994 Association of four antigens of the tetraspans family (CD37, CD53, TAPA-1, and R2/C33) with MHC class II glycoproteins. Immunogenetics 165 8119731
2019 H4K20me0 recognition by BRCA1-BARD1 directs homologous recombination to sister chromatids. Nature cell biology 162 30804502
2003 GLP-2-mediated up-regulation of intestinal blood flow and glucose uptake is nitric oxide-dependent in TPN-fed piglets 1. Gastroenterology 162 12851879
2000 GLP-2 stimulates intestinal growth in premature TPN-fed pigs by suppressing proteolysis and apoptosis. American journal of physiology. Gastrointestinal and liver physiology 156 11093948
2005 Human leukocyte antigen and antigen processing machinery component defects in astrocytic tumors. Clinical cancer research : an official journal of the American Association for Cancer Research 154 16322289
2005 Varicelloviruses avoid T cell recognition by UL49.5-mediated inactivation of the transporter associated with antigen processing. Proceedings of the National Academy of Sciences of the United States of America 147 15793001
2005 Tapasin and ERp57 form a stable disulfide-linked dimer within the MHC class I peptide-loading complex. The EMBO journal 144 16193070
2009 Ubiquitin-mediated proteolysis of HuR by heat shock. The EMBO journal 142 19322201
1999 The N-terminal region of tapasin is required to stabilize the MHC class I loading complex. European journal of immunology 142 10382748
1990 TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen. Journal of immunology (Baltimore, Md. : 1950) 141 2398277
2006 Redox regulation facilitates optimal peptide selection by MHC class I during antigen processing. Cell 130 17055437
2017 An Interaction Landscape of Ubiquitin Signaling. Molecular cell 119 28190767
1996 Point mutations in the alpha 2 domain of HLA-A2.1 define a functionally relevant interaction with TAP. Current biology : CB 118 8805302
2009 High-density SNP screening of the major histocompatibility complex in systemic lupus erythematosus demonstrates strong evidence for independent susceptibility regions. PLoS genetics 109 19851445
2021 Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling. Molecular cell 105 33545068
1997 Cloning and functional characterization of a subunit of the transporter associated with antigen processing. Proceedings of the National Academy of Sciences of the United States of America 104 9238042
1992 The effect of glutamine-enriched TPN on gut immune cellularity. The Journal of surgical research 103 1548865
1993 The CD19 signal transduction complex of B lymphocytes. Deletion of the CD19 cytoplasmic domain alters signal transduction but not complex formation with TAPA-1 and Leu 13. Journal of immunology (Baltimore, Md. : 1950) 101 7690791
2004 Onset of small intestinal atrophy is associated with reduced intestinal blood flow in TPN-fed neonatal piglets. The Journal of nutrition 98 15173413
1997 A temporal study of TPN-induced changes in gut-associated lymphoid tissue and mucosal immunity. Archives of surgery (Chicago, Ill. : 1960) 88 9403534
1987 Failure of TPN supplementation to improve liver function, immunity, and mortality in thermally injured patients. The Journal of trauma 81 3102754
2001 TPN decreases IL-4 and IL-10 mRNA expression in lipopolysaccharide stimulated intestinal lamina propria cells but glutamine supplementation preserves the expression. Shock (Augusta, Ga.) 75 11303733
1993 The CD19-CR2-TAPA-1 complex, CD45 and signaling by the antigen receptor of B lymphocytes. Current opinion in immunology 75 7688513
1993 The TAPA-1 molecule is associated on the surface of B cells with HLA-DR molecules. Journal of immunology (Baltimore, Md. : 1950) 73 8409388
1991 Structure and membrane topology of TAPA-1. The Journal of biological chemistry 71 1860863
1993 Anti-TAPA-1 antibodies induce protein tyrosine phosphorylation that is prevented by increasing intracellular thiol levels. Journal of immunology (Baltimore, Md. : 1950) 65 7688390
2014 Functional analysis of the accessory protein TapA in Bacillus subtilis amyloid fiber assembly. Journal of bacteriology 63 24488317
1996 Astrocyte growth, reactivity, and the target of the antiproliferative antibody, TAPA. The Journal of neuroscience : the official journal of the Society for Neuroscience 59 8757260
1984 Protein metabolism during total parenteral nutrition (TPN) in injured rats using medium-chain triglycerides. Metabolism: clinical and experimental 59 6434897
1991 Genomic organization and chromosomal localization of the TAPA-1 gene. Journal of immunology (Baltimore, Md. : 1950) 56 1650385
1993 Small intestinal mucosa changes, including epithelial cell proliferative activity, of children receiving total parenteral nutrition (TPN). Digestive diseases and sciences 51 8359071
1981 Protein and fat metabolism in rats during repletion with total parenteral nutrition (TPN). The Journal of nutrition 51 6778978
2002 A human TAPBP (TAPASIN)-related gene, TAPBP-R. European journal of immunology 49 11920573
2002 Alterations in enterocyte proliferation and apoptosis accompany TPN-induced mucosal hypoplasia and IGF-I-induced hyperplasia in rats. The Journal of nutrition 47 12097684
1983 Effect of postoperative total parenteral nutrition (TPN) as an adjunct to gastrectomy for advanced gastric carcinoma. The British journal of surgery 44 6405838
2004 Increased expression of specific intestinal amino acid and peptide transporter mRNA in rats fed by TPN is reversed by GLP-2. The Journal of nutrition 42 15514259
1982 Protein sparing and protein replacement in acutely injured patients during TPN with and without amino acid supply. Intensive care medicine 39 6799558
2003 Combined growth hormone/insulin-like growth factor I in addition to glutamine-supplemented TPN results in net protein anabolism in critical illness. American journal of physiology. Endocrinology and metabolism 33 12759221
2001 Modulation of organ ICAM-1 expression during IV-TPN with glutamine and bombesin. Shock (Augusta, Ga.) 33 11198353
1977 Identification of D-threo-alpha-methylisocitrate as stereochemically specific substrate for bovine heart aconitase and inhibitor of TPN-linked isocitrate dehydrogenase. The Journal of biological chemistry 33 856801
1983 Modification of TPN-dependent isocitrate dehydrogenase by the 2', 3'-dialdehyde derivatives of TPNH and TPN. The Journal of biological chemistry 32 6874692
2000 Up-regulation of CD81 (target of the antiproliferative antibody; TAPA) by reactive microglia and astrocytes after spinal cord injury in the rat. The Journal of comparative neurology 31 11064366
1998 Delivery of total parenteral nutrition (TPN) via umbilical catheterization: development of a piglet model to investigate therapies to improve gastrointestinal structure and enzyme activity during TPN. Biology of the neonate 30 9573459
1998 Expression of rat target of the antiproliferative antibody (TAPA) in the developing brain. The Journal of comparative neurology 28 9624590
1975 Events Surrounding the Early Development of Euglena Chloroplasts: VI. Action Spectra for the Formation of Chlorophyll, Lag Elimination in Chlorophyll Synthesis, and Appearance of TPN-dependent Triose Phosphate Dehydrogenase and Alkaline DNase Activities. Plant physiology 28 16659294
2004 Effects of lipid administration on liver apoptotic signals in a mouse model of total parenteral nutrition (TPN). Pediatric surgery international 27 15034728
2015 TPN-associated intestinal epithelial cell atrophy is modulated by TLR4/EGF signaling pathways. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24 25782989
2003 Blood-brain barrier transport of a novel micro 1-specific opioid peptide, H-Tyr-D-Arg-Phe-beta-Ala-OH (TAPA). Journal of neurochemistry 24 12603838
2003 Changes in epithelial cell turnover and extracellular matrix in human small intestine after TPN. The Journal of surgical research 23 12643847
2002 Photoprotection prevents TPN-induced lung procollagen mRNA in newborn guinea pigs. Free radical biology & medicine 23 12160933
2009 Comparison of two types of TPN prescription methods in preterm neonates. Pharmacy world & science : PWS 22 19169838
2020 The majority of the matrix protein TapA is dispensable for Bacillus subtilis colony biofilm architecture. Molecular microbiology 21 32491277
1988 Inhibition of TPN-associated intestinal mucosal atrophy with monoacetoacetin. The Journal of surgical research 21 3129616
1984 Serum lipids and fatty acids composition of tissues in rats on total parenteral nutrition (TPN). Lipids 21 6438428
1984 Protein dynamics during refeeding of protein-depleted rats: effects of increasing amino acid intake by TPN or enteral continuous feeding. The Journal of nutrition 20 6420525
2013 Treatment of diabetes mellitus with microencapsulated fetal human liver (FH-B-TPN) engineered cells. Biomaterials 19 23453199
1996 Ligation of TAPA-1 (CD81) or major histocompatibility complex class II in co-cultures of human B and T lymphocytes enhances interleukin-4 synthesis by antigen-specific CD4+ T cells. European journal of immunology 19 8766544
2009 Up-regulation of intestinal Toll-Like receptors and cytokines expressions change after TPN administration and a lack of enteral feeding. The Journal of surgical research 18 19524259
2016 Multi-omic profiles of hepatic metabolism in TPN-fed preterm pigs administered new generation lipid emulsions. Journal of lipid research 17 27474222
1991 Zinc and copper status of severely burned children during TPN. Journal of the American College of Nutrition 17 1901324
1988 Inhibition of post-traumatic septic proteolysis and ureagenesis and stimulation of hepatic acute-phase protein production by branched-chain amino acid TPN. The Journal of trauma 16 2457711
2023 TapA acts as specific chaperone in TasA filament formation by strand complementation. Proceedings of the National Academy of Sciences of the United States of America 15 37068239
2016 Generating hESCs with reduced immunogenicity by disrupting TAP1 or TAPBP. Bioscience, biotechnology, and biochemistry 15 27068360
1997 Pyrogen testing of lipid-based TPN using Mono Mac 6 monocyte cell line and DELFIA. Journal of clinical pharmacy and therapeutics 14 19160716
1996 Triggering of target of an antiproliferative antibody-1 (TAPA-1/CD81) up-regulates the release of tumour necrosis factor-alpha by the EBV-B lymphoblastoid cell line JY. Scandinavian journal of immunology 14 8668914
1984 Trace metal requirements in total parenteral nutrition (TPN). 5. Formation constants for the copper(II)--histidine ternary complexes with threonine, lysine, glycine, phenylalanine, valine, and cystine, and discussion of their implications regarding the copper distribution in blood plasma during TPN and the evaluation of the daily dose of copper. Journal of inorganic biochemistry 14 6425456
2018 The Bacterial Extracellular Matrix Protein TapA Is a Two-Domain Partially Disordered Protein. Chembiochem : a European journal of chemical biology 13 30371005
1999 The expression of TAPA (CD81) correlates with the reactive response of astrocytes in the developing rat CNS. Experimental neurology 13 10619563
1998 The target of the antiproliferative antibody (TAPA) in the normal and injured rat retina. Molecular vision 13 9485486
1983 Cellular localization of liver vitamin A in rats given total parenteral nutrition (TPN) solutions intravenously or orally. The Journal of nutrition 13 6406651
2010 Lack of a rewarding effect and a locomotor-enhancing effect of the selective μ-opioid receptor agonist amidino-TAPA. Psychopharmacology 12 20683583
2007 Involvement of endogenous opioid peptides in the antinociception induced by the novel dermorphin tetrapeptide analog amidino-TAPA. European journal of pharmacology 12 17307162
1992 Expression of TAPA-1 in preimplantation mouse embryos. Biochemical and biophysical research communications 12 1380797
1975 Alpha-methylisocitrate. A selective inhibitor of TPN-linked isocitrate dehydrogenase from bovine heart and rat liver. The Journal of biological chemistry 12 239945
2002 Retinal pigment epithelium of the rat express CD81, the target of the anti-proliferative antibody (TAPA). Investigative ophthalmology & visual science 11 11773042
2002 Crystal structures of two rat MHC class Ia (RT1-A) molecules that are associated differentially with peptide transporter alleles TAP-A and TAP-B. Journal of molecular biology 11 12470953
1985 Post-operative substrate utilisation and gas exchange using two different TPN-systems: glucose versus fat. Clinical nutrition (Edinburgh, Scotland) 11 16831738
2020 Computational investigation for modeling the protein-protein interaction of TasA(28-261)-TapA(33-253): a decisive process in biofilm formation by Bacillus subtilis. Journal of molecular modeling 10 32779018
2007 Variations in metabolic response to TPN are influenced more by sex than by light exposure. Journal of pediatric gastroenterology and nutrition 10 18030236
2001 TPN-evoked dysfunction of islet lysosomal activity mediates impairment of glucose-stimulated insulin release. American journal of physiology. Endocrinology and metabolism 10 11404235
2016 Preoperative overnight parenteral nutrition (TPN) improves skeletal muscle protein metabolism indicated by microarray algorithm analyses in a randomized trial. Physiological reports 9 27273879
2005 Postoperative nutritional management after esophagectomy: is TPN the standard of nutritional care? International surgery 9 15912897
1977 Structure-function relationships in TPN-dependent isocitrate dehydrogenase. I. Electron paramagnetic resonance studies of the interaction of enzyme-bound Mn(II) with substrates, cofactors, and substrate analogues. Biochemistry 9 19044
2004 Candida parapsilosis detected in TPN using the BacT/Alert system and characterized by randomly amplified polymorphic DNA. The Journal of hospital infection 8 15066740
1987 The effect of total parenteral nutrition (TPN) on gastrin release in the rat. Regulatory peptides 8 3125565
1984 Does total parenteral nutrition (TPN) really promote tumor growth? A morphometric study. Cancer 8 6434177
2016 A peptide tetramer Tk-tPN induces tolerance of cardiac allografting by conversion of type 1 to type 2 immune responses via the Toll-like receptor 2 signal-promoted activation of the MCP1 gene. Immunology 7 26694804
1985 The effect of total parenteral nutrition (TPN) on the enteroinsular axis in the rat. Regulatory peptides 7 3922014
2005 The role of polyamines in glucagon-like peptide-2 prevention of TPN-induced gut hypoplasia. Peptides 6 16274854
1995 Induction of heat-shock proteins and accumulation of trehalose by TPN in Saccharomyces cerevisiae. Biochemical and biophysical research communications 6 7488177
1991 Nitrogen and energy balance in depleted patients undergoing major gastrointestinal surgery: response to TPN. Clinical nutrition (Edinburgh, Scotland) 6 16839892
2021 Inadvertent Acute Lipid Injectable Emulsion Overdose Resulting in Fat Overload Syndrome and Pancreatitis in a Patient with TPN Dependence. JPGN reports 5 37168746
2010 Dose variation of hepatocyte growth factor and its effects on an animal model of TPN-induced liver injury. The Journal of surgical research 5 20691983
2010 Involvement of mouse μ-opioid receptor splice variants in the spinal antinociception induced by the dermorphin tetrapeptide analog amidino-TAPA. European journal of pharmacology 5 21047509
2003 Homocysteine prevents total parenteral nutrition (TPN)-induced cholestasis without changes in hepatic oxidative stress in the rat. Journal of pediatric gastroenterology and nutrition 5 12548054
1990 Glucose-based versus fat-based total parenteral nutrition (TPN): effects on hepatic function in septic patients complicated with cholestatic jaundice. Clinical nutrition (Edinburgh, Scotland) 5 16837362
2024 A genetic variant in the TAPBP gene enhances cervical cancer susceptibility by increasing m6A modification. Archives of toxicology 4 38992170
2022 Activity-Oriented Antiedema Proprioceptive Therapy (TAPA) for Shoulder Mobility Improvement in Women with Upper Limb Lymphedema Secondary to Breast Cancer: A Multicenter Controlled Clinical Trial. Journal of clinical medicine 4 35456327
2013 Involvement of spinal release of α-neo-endorphin on the antinociceptive effect of TAPA. Peptides 4 24126280
1986 Effect of DL-3-hydroxybutyrate infusions on leucine and glucose kinetics in burned rats receiving TPN. The Journal of nutrition 4 3080557
2016 In-vitro detection of mannan and galactomannan in components of total parenteral nutrition (TPN). Die Pharmazie 3 27348965
1988 Effect of TPN on bone marrow kinetics--therapeutic implications. The Journal of surgical research 3 3138500
2013 Distinct physiological role of amidino-TAPA-sensitive and DAMGO-insensitive μ-opioid receptor splice variants in the mouse spinal cord. European journal of pharmacology 2 23623932
1998 Effects of total parental nutrition (TPN) during high-dose interleukin-2 treatment for metastatic cancer. Journal of immunotherapy (Hagerstown, Md. : 1997) 2 9456439
1985 Glucose balance and muscle glycogen during TPN in the early post-operative phase. Clinical nutrition (Edinburgh, Scotland) 2 16831739