Affinage

STX2

Syntaxin-2 · UniProt P32856

Length
288 aa
Mass
33.3 kDa
Annotated
2026-06-13
100 papers in source corpus

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

No mechanistic discoveries found in literature.

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
No controlled-vocabulary terms were assigned to this entry.

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2004 Structure of shiga toxin type 2 (Stx2) from Escherichia coli O157:H7. The Journal of biological chemistry 218 15075327
1998 Non-O157:H7 Stx2-producing Escherichia coli strains associated with sporadic cases of hemolytic-uremic syndrome in adults. Journal of clinical microbiology 119 9620420
2004 Diversity of stx2 converting bacteriophages induced from Shiga-toxin-producing Escherichia coli strains isolated from cattle. Microbiology (Reading, England) 105 15347754
2003 Detection of bacteria carrying the stx2 gene by in situ loop-mediated isothermal amplification. Applied and environmental microbiology 97 12902306
2004 Interaction of Shiga toxin from Escherichia coli with human intestinal epithelial cell lines and explants: Stx2 induces epithelial damage in organ culture. Cellular microbiology 88 14764112
2003 Strong association between shiga toxin-producing Escherichia coli O157 and virulence genes stx2 and eae as possible explanation for predominance of serogroup O157 in patients with haemolytic uraemic syndrome. European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology 74 14614596
2008 Differential expression of stx2 variants in Shiga toxin-producing Escherichia coli belonging to seropathotypes A and C. Microbiology (Reading, England) 73 18174136
2001 Stx2 subtyping of Shiga toxin-producing Escherichia coli isolated from cattle in France: detection of a new Stx2 subtype and correlation with additional virulence factors. Journal of clinical microbiology 72 11526129
1999 Induction of cytokines in a human colon epithelial cell line by Shiga toxin 1 (Stx1) and Stx2 but not by non-toxic mutant Stx1 which lacks N-glycosidase activity. FEBS letters 69 9929007
2003 Multiplex real-time PCR method to identify Shiga toxin genes stx1 and stx2 and Escherichia coli O157:H7/H- serotype. Applied and environmental microbiology 67 14532101
2008 Mouse model of hemolytic-uremic syndrome caused by endotoxin-free Shiga toxin 2 (Stx2) and protection from lethal outcome by anti-Stx2 antibody. Infection and immunity 62 18694970
2002 Shiga toxin-producing Escherichia coli infection and antibodies against Stx2 and Stx1 in household contacts of children with enteropathic hemolytic-uremic syndrome. Journal of clinical microbiology 58 11980959
2007 Insertion site occupancy by stx2 bacteriophages depends on the locus availability of the host strain chromosome. Journal of bacteriology 57 17644594
2007 Prevalence of Shiga toxin-producing Escherichia coli stx1, stx2, eaeA, and rfbE genes and survival of E. coli O157:H7 in manure from organic and low-input conventional dairy farms. Applied and environmental microbiology 55 17277204
2001 Neutralization of Shiga toxins Stx1, Stx2c, and Stx2e by recombinant bacteria expressing mimics of globotriose and globotetraose. Infection and immunity 52 11179385
2010 Human isolates of Aeromonas possess Shiga toxin genes (stx1 and stx2) highly similar to the most virulent gene variants of Escherichia coli. Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases 42 20219084
2002 Experimental induction of equine protozoan myeloencephalitis (EPM) in the horse: effect of Sarcocystis neurona sporocyst inoculation dose on the development of clinical neurologic disease. The Journal of parasitology 40 12537112
1999 Sequence analysis of Stx2-converting phage VT2-Sa shows a great divergence in early regulation and replication regions. DNA research : an international journal for rapid publication of reports on genes and genomes 39 10492170
2004 Prevalence of the stx2 gene in coliform populations from aquatic environments. Applied and environmental microbiology 38 15184154
2000 Inducible stx2 phages are lysogenized in the enteroaggregative and other phenotypic Escherichia coli O86:HNM isolated from patients. FEMS microbiology letters 37 11004392
2017 Population structure of Escherichia coli O26 : H11 with recent and repeated stx2 acquisition in multiple lineages. Microbial genomics 36 29208163
2006 Genetic characterization of Escherichia coli O157: H7/- strains carrying the stx2 gene but not producing Shiga toxin 2. Microbiology and immunology 36 16490932
2009 Enterohemorrhagic Escherichia coli trivalent recombinant vaccine containing EspA, intimin and Stx2 induces strong humoral immune response and confers protection in mice. Microbes and infection 35 19427397
2014 The presence of the pAA plasmid in the German O104:H4 Shiga toxin type 2a (Stx2a)-producing enteroaggregative Escherichia coli strain promotes the translocation of Stx2a across an epithelial cell monolayer. The Journal of infectious diseases 34 25038258
2011 Shiga toxin (Stx)1B and Stx2B induce von Willebrand factor secretion from human umbilical vein endothelial cells through different signaling pathways. Blood 34 21816831
2009 Monoclonal antibody 11E10, which neutralizes shiga toxin type 2 (Stx2), recognizes three regions on the Stx2 A subunit, blocks the enzymatic action of the toxin in vitro, and alters the overall cellular distribution of the toxin. Infection and immunity 34 19433543
2013 Oral intoxication of mice with Shiga toxin type 2a (Stx2a) and protection by anti-Stx2a monoclonal antibody 11E10. Infection and immunity 33 24379294
2012 Spread of a distinct Stx2-encoding phage prototype among Escherichia coli O104:H4 strains from outbreaks in Germany, Norway, and Georgia. Journal of virology 33 22811533
2009 Shiga toxin Stx2 is heat-stable and not inactivated by pasteurization. International journal of food microbiology 33 19875188
2012 Carriage of stx2a differentiates clinical and bovine-biased strains of Escherichia coli O157. PloS one 32 23240045
2011 Subcutaneous and intranasal immunization with Stx2B-Tir-Stx1B-Zot reduces colonization and shedding of Escherichia coli O157:H7 in mice. Vaccine 32 21338683
2014 New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo. PloS one 31 24914553
2008 Genotypic and phenotypic diversity among induced, stx2-carrying bacteriophages from environmental Escherichia coli strains. Applied and environmental microbiology 31 19011056
2003 Genome analysis of a novel Shiga toxin 1 (Stx1)-converting phage which is closely related to Stx2-converting phages but not to other Stx1-converting phages. Journal of bacteriology 30 12813092
2012 High temperature in combination with UV irradiation enhances horizontal transfer of stx2 gene from E. coli O157:H7 to non-pathogenic E. coli. PloS one 28 22347461
2009 Immunogenicity of a novel Stx2B-Stx1B fusion protein in a mice model of Enterohemorrhagic Escherichia coli O157:H7 infection. Vaccine 28 19428832
2015 Development and characterization of recombinant antibody fragments that recognize and neutralize in vitro Stx2 toxin from Shiga toxin-producing Escherichia coli. PloS one 26 25790467
2013 Shiga toxin-producing Escherichia coli strains from cattle as a source of the Stx2a bacteriophages present in enteroaggregative Escherichia coli O104:H4 strains. International journal of medical microbiology : IJMM 26 24012149
2015 A Four-Plex Real-Time PCR Assay, Based on rfbE, stx1, stx2, and eae Genes, for the Detection and Quantification of Shiga Toxin-Producing Escherichia coli O157 in Cattle Feces. Foodborne pathogens and disease 25 26317538
2013 t-SNARE Syntaxin2 (STX2) is implicated in intracellular transport of sulfoglycolipids during meiotic prophase in mouse spermatogenesis. Biology of reproduction 25 23595907
2005 Detection of Shiga toxin genes stx1, stx2, and the +93 uidA mutation of E. coli O157:H7/H-using SYBR Green I in a real-time multiplex PCR. Molecular and cellular probes 25 16271448
2018 Structural Basis for the Specific Neutralization of Stx2a with a Camelid Single Domain Antibody Fragment. Toxins 24 29494518
2018 STX2 promotes colorectal cancer metastasis through a positive feedback loop that activates the NF-κB pathway. Cell death & disease 24 29855462
2014 Protection of mice against Shiga toxin 2 (Stx2)-associated damage by maternal immunization with a Brucella lumazine synthase-Stx2 B subunit chimera. Infection and immunity 24 24421050
2006 Binding of adenine to Stx2, the protein toxin from Escherichia coli O157:H7. Acta crystallographica. Section F, Structural biology and crystallization communications 24 16820678
2015 Synthetic Studies on Centromere-Associated Protein-E (CENP-E) Inhibitors: 2. Application of Electrostatic Potential Map (EPM) and Structure-Based Modeling to Imidazo[1,2-a]pyridine Derivatives as Anti-Tumor Agents. Journal of medicinal chemistry 22 26372373
2009 Risk factors for the occurrence of Escherichia coli virulence genes eae, stx1 and stx2 in wild bird populations. Epidemiology and infection 22 19327201
2021 STX2 Promotes Trophoblast Growth, Migration, and Invasion Through Activation of the PI3K-AKT Pathway in Preeclampsia. Frontiers in cell and developmental biology 20 34295885
2013 Role of TNF-α in the mechanisms responsible for preterm delivery induced by Stx2 in rats. British journal of pharmacology 20 23043728
2009 Phylogroups, virulence determinants and antimicrobial resistance in stx(2) gene-carrying Escherichia coli isolated from aquatic environments. Research in microbiology 20 19723578
2014 Enhanced virulence of the Escherichia coli O157:H7 spinach-associated outbreak strain in two animal models is associated with higher levels of Stx2 production after induction with ciprofloxacin. Infection and immunity 19 25225244
2012 Evolution of the Stx2-encoding prophage in persistent bovine Escherichia coli O157:H7 strains. Applied and environmental microbiology 19 23275514
2017 The Mobilome; A Major Contributor to Escherichia coli stx2-Positive O26:H11 Strains Intra-Serotype Diversity. Frontiers in microbiology 18 28932209
2011 A sensitive and simple plaque formation method for the Stx2 phage of Escherichia coli O157:H7, which does not form plaques in the standard plating procedure. Plasmid 18 22186359
2003 Detection, enumeration and isolation of strains carrying the stx2 gene from urban sewage. Water science and technology : a journal of the International Association on Water Pollution Research 18 12639014
2016 Expression of Shiga toxin 2 (Stx2) in highly virulent Stx-producing Escherichia coli (STEC) carrying different anti-terminator (q) genes. Microbial pathogenesis 17 27208749
2012 H1-histamine receptors in the amygdala are involved in emotional memory but do not mediate anxiety-related behaviors in mice submitted to EPM testing. Brain research bulletin 16 22772356
2010 Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immunomagnetic separation technique and stx1 and stx2 genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey. Journal of veterinary science 16 21113101
2008 Production and characterization of rabbit polyclonal sera against Shiga toxins Stx1 and Stx2 for detection of Shiga toxin-producing Escherichia coli. Microbiology and immunology 16 18822082
2018 STX2 is a causative gene for nonobstructive azoospermia. Human mutation 15 29570232
2008 A new ENU-induced mutant mouse with defective spermatogenesis caused by a nonsense mutation of the syntaxin 2/epimorphin (Stx2/Epim) gene. The Journal of reproduction and development 15 18277055
2013 Promoter sequence of Shiga toxin 2 (Stx2) is recognized in vivo, leading to production of biologically active Stx2. mBio 14 24085779
2003 Epidemiology of Sarcocystis neurona infections in domestic cats (Felis domesticus) and its association with equine protozoal myeloencephalitis (EPM) case farms and feral cats from a mobile spay and neuter clinic. Veterinary parasitology 14 14637026
1987 A common epithelial cell surface antigen (EPM-1) on gastrointestinal tumors and in human sera. Cancer research 14 3594445
2019 Recombinant HcpA-EspA-Tir-Stx2B chimeric protein induces immunity against attachment and toxicity of Escherichia coli O157:H7. Microbial pathogenesis 13 30731187
2018 Non-pathogenic Escherichia coli Enhance Stx2a Production of E. coli O157:H7 Through Both bamA-Dependent and Independent Mechanisms. Frontiers in microbiology 13 29973923
2016 Immunogenic properties of trivalent recombinant protein composed of B-subunits of LT, STX-2, and CT toxins. Microbes and infection 13 26970204
2008 Subtype analysis of stx1, stx2 and eae genes in Shiga toxin-producing Escherichia coli (STEC) and typical and atypical enteropathogenic E. coli (EPEC) from lambs in India. Veterinary journal (London, England : 1997) 13 18768338
2018 Nano-multilamellar lipid vesicles (NMVs) enhance protective antibody responses against Shiga toxin (Stx2a) produced by enterohemorrhagic Escherichia coli strains (EHEC). Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 12 30637647
2017 H-NS Mutation-Mediated CRISPR-Cas Activation Inhibits Phage Release and Toxin Production of Escherichia coli Stx2 Phage Lysogen. Frontiers in microbiology 12 28458663
2008 Isolation and characterization of the Shiga toxin gene (stx)-bearing Escherichia coli O157 and non-O157 from retail meats in Shandong Province, China, and characterization of the O157-derived stx2 phages. Journal of food protection 12 18468023
2003 Role of Shiga toxin 2 (Stx2)-binding protein, human serum amyloid P component (HuSAP), in Shiga toxin-producing Escherichia coli infections: assumption from in vitro and in vivo study using HuSAP and anti-Stx2 humanized monoclonal antibody TMA-15. Biochemical and biophysical research communications 12 12767937
2018 ArgO145, a Stx2a prophage of a bovine O145:H- STEC strain, is closely related to phages of virulent human strains. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases 10 29476813
2011 Assessment of transduction of Escherichia coli Stx2-encoding phage in dairy process conditions. International journal of food microbiology 10 22197444
2009 Antimicrobial resistance and virulence factors stx1, stx2, and eae in generic Escherichia coli isolates from calves in western Canadian cow-calf herds. Microbial drug resistance (Larchmont, N.Y.) 10 19216645
2002 Identification of Escherichia coli O157:H7 by multiplex PCR with primers specific to the hlyA, eaeA, stx1, stx2, fliC and rfb genes. Journal of the Formosan Medical Association = Taiwan yi zhi 10 12645196
2020 Development and Evaluation of a Novel VHH-Based Immunocapture Assay for High-Sensitivity Detection of Shiga Toxin Type 2 (Stx2) in Stool Samples. Journal of clinical microbiology 9 31826960
1990 Expression of epithelial antigens Exo-1 and EPM-1 in human epidermal keratinocyte maturation and benign and malignant neoplasia. Cancer research 9 2253212
2021 In silico analysis of STX2a-PE15-P4A8 chimeric protein as a novel immunotoxin for cancer therapy. In silico pharmacology 8 33643767
2020 STX2 drives colorectal cancer proliferation via upregulation of EXOSC4. Life sciences 8 33075373
2017 PMicroRNA-124a regulates LPS-induced septic cardiac dysfunction by targeting STX2. Biotechnology letters 8 28560580
1994 Linkage analysis of idiopathic generalised epilepsy in families of probands with Juvenile Myoclonic Epilepsy and marker loci in the region of EPM 1 on chromosome 21 q: Unverricht-Lundborg disease and JME are not allelic variants. Neuropediatrics 8 8208346
2016 Immunization with BLS-Stx2B chimera totally protects dams from early pregnancy loss induced by Shiga toxin type 2 (Stx2) and confers anti-Stx2 immunity to the offspring. Vaccine 7 27527816
2015 New Stx2e Monoclonal Antibodies for Immunological Detection and Distinction of Stx2 Subtypes. PloS one 7 26192407
2015 Draft Genome Sequences of Human-Pathogenic Escherichia coli O26:H11 Strains Carrying the stx2 Gene Only and Circulating in France. Genome announcements 7 26227606
2014 Humoral immune response to Shiga Toxin 2 (Stx2) among Brazilian urban children with hemolytic uremic syndrome and healthy controls. BMC infectious diseases 7 24919599
2011 Intranasal immunisation with Stx2B-Tir-Stx1B-Zot protein leads to decreased shedding in goats after challenge with Escherichia coli O157:H7. The Veterinary record 7 22186381
2009 Improved production of holotoxin Stx2 with biological activities by using a single-promoter vector and an auto-induction expression system. Protein expression and purification 7 19450690
2014 Microwave Heating Inactivates Shiga Toxin (Stx2) in Reconstituted Fat-Free Milk and Adversely Affects the Nutritional Value of Cell Culture Medium. Journal of agricultural and food chemistry 6 24669932
2010 Characterization of stx2 tubular response in a rat experimental model of hemolytic uremic syndrome. American journal of nephrology 6 20733289
2024 Discovery and design of an aptamer that inhibits Shiga toxin type 2 activity by blocking Stx2 B subunit-Gb3 interaction. International journal of biological macromolecules 5 39089540
2015 Natural killer T (NKT) cells accelerate Shiga toxin type 2 (Stx2) pathology in mice. Frontiers in microbiology 5 25904903
2010 Rapid detection of virulence stx2 gene of Enterohemorrhagic Escherichia coli using two-step ultra-rapid real-time PCR. Biotechnology letters 5 20364295
2009 Antibody responses elicited in mice immunized with Bacillus subtilis vaccine strains expressing Stx2B subunit of enterohaemorragic Escherichia coli O157:H7. Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 5 24031368
2023 Shiga Toxin (Stx) Type 1a and Stx2a Translocate through a Three-Layer Intestinal Model. Toxins 4 36977098
2022 Isolation of a multidrug-resistant Escherichia coli pathotype Stx2:Cnf1:Cnf2:Eae as a potential cause of hemorrhagic diarrhea and secondary septicemia in a dog. Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 4 35075964
2021 The Deleterious Effects of Shiga Toxin Type 2 Are Neutralized In Vitro by FabF8:Stx2 Recombinant Monoclonal Antibody. Toxins 4 34822608
2019 Endocytosis, Cytotoxicity, and Translocation of Shiga Toxin-2 Are Stimulated by Infection of Human Intestinal (HCT-8) Monolayers With an Hypervirulent E. coli O157:H7 Lacking stx2 Gene. Frontiers in cellular and infection microbiology 4 31824869
2015 Shiga toxin type-2 (Stx2) induces glutamate release via phosphoinositide 3-kinase (PI3K) pathway in murine neurons. Frontiers in molecular neuroscience 4 26236186
2008 Detection and frequency of Stx2 gene in Escherichia coli O157 and O157:H7 strains isolated from sheep carcasses in Shiraz-Iran. Pakistan journal of biological sciences : PJBS 4 18819545

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