| 2006 |
SRp54/SFRS11 (SRSF11) acts as a repressor of tau exon 10 inclusion: overexpression suppresses exon 10 inclusion, siRNA knockdown increases it. SRSF11 binds a purine-rich element in tau exon 10 and antagonizes Tra2beta, an SR-domain protein that promotes exon 10 inclusion. Deletion of the exonic purine-rich element abolishes SRSF11 repressor activity. |
GFP-based exon-skipping reporter assay, expression cloning, RNA interference knockdown, deletion mutagenesis of exonic element, functional antagonism assay with Tra2beta |
Molecular and cellular biology |
High |
16943417
|
| 2015 |
SRSF11 localizes to nuclear speckles and functions as a nuclear speckle-targeting factor for telomerase. It binds TERC (telomerase RNA component) directly and also binds TRF2 at telomeres. During S phase, SRSF11 directs active telomerase to nuclear speckles; a subset of telomeres resides constitutively at nuclear speckles. Depletion of SRSF11 prevents telomerase association with nuclear speckles, disrupts telomerase recruitment to telomeres, and abrogates telomere elongation. |
Co-immunoprecipitation (SRSF11 with TERC and TRF2), subcellular fractionation/immunofluorescence showing nuclear speckle localization, cell-cycle-specific analysis (S-phase), SRSF11 depletion with telomere elongation readout |
Nucleic acids research |
High |
26286192
|
| 2019 |
SRSF11 directly binds the 3' UTR of LRP8 mRNA and the third exon of apoE mRNA, stabilizing both transcripts. Loss of SFRS11/SRSF11 reduces LRP8 and apoE levels, leading to activation of JNK signaling. Restoration of LRP8 and apoE reduces JNK signaling elevated in SFRS11-deficient cells and rescues aging-like cognitive phenotypes. |
RNA immunoprecipitation (RIP) to confirm direct mRNA binding, mRNA stability assays, genetic rescue experiments (LRP8/apoE restoration in SFRS11-deficient cells), JNK signaling readout, prefrontal cortex-specific knockdown in mice with cognitive behavioral tests |
Cell reports |
High |
31269452
|
| 2022 |
SRSF11 promotes colorectal cancer metastasis by directly binding a motif in exon 2 of HSPA12A pre-mRNA (identified by UV-CLIP and minigene reporter), causing exon 2 retention and increased HSPA12A transcript stability, which in turn elevates N-cadherin expression. The oncogenic kinase PAK5 phosphorylates SRSF11 at serine 287, protecting it from ubiquitin-mediated degradation. |
In vivo UV crosslinking and immunoprecipitation (CLIP), minigene reporter assay, Co-immunoprecipitation, Phospho-tag SDS-PAGE, in vitro kinase assay (PAK5 phosphorylating SRSF11 at S287), RNA-seq for AS events, in vitro and in vivo metastasis models |
Clinical and translational medicine |
High |
36394206
|
| 2025 |
KAT2A directly interacts with SRSF11 and catalyzes succinylation of SRSF11 at lysine 419 (K419). K419 succinylation stabilizes SRSF11-spliceosome interactions and promotes inclusion of exon 10 of RAD52 pre-mRNA, preserving the RAD51-binding domain of RAD52. This facilitates RAD52-RAD51 dimer assembly and homologous recombination repair, leading to radioresistance in hepatocellular carcinoma. |
Co-immunoprecipitation (KAT2A-SRSF11 interaction), structural and functional analyses of K419 succinylation, pre-mRNA binding assays, exon inclusion assays (RAD52 exon 10), in vitro and in vivo HCC models, genetic disruption of KAT2A-SRSF11 axis with radiation sensitivity readout |
Signal transduction and targeted therapy |
Medium |
41198615
|
| 2025 |
miR-126-5p delivered by M1 macrophage exosomes targets and inhibits SRSF11 expression. SRSF11 binds Sirt1 and P21 precursor mRNAs (confirmed by RIP), and its inhibition leads to dysregulation of alternative splicing of these genes with an increase in pro-senescence isoforms, driving endothelial cell senescence. |
RIP experiments confirming SRSF11 binding to Sirt1/P21 pre-mRNA, exosome isolation and characterization, luciferase/miRNA target validation, SA-β-gal staining and flow cytometry for senescence, in vivo DVT mouse model |
International immunopharmacology |
Medium |
41343940
|
| 2025 |
SRSF11 and SRRM1 share common protein interactors and RNA targets and together promote oncogenic NUMB exon 9 (E9) splicing in colorectal, lung, and breast cancer cell lines, identified in a genome-wide CRISPR screen using a NUMB E9 splicing reporter. |
Genome-wide CRISPR screen with NUMB exon 9 splicing reporter, protein interaction studies, RNA target analysis |
bioRxivpreprint |
Low |
bio_10.1101_2025.08.20.671097
|