Affinage

SLBP

Histone RNA hairpin-binding protein · UniProt Q14493

Length
270 aa
Mass
31.3 kDa
Annotated
2026-04-28
100 papers in source corpus 22 papers cited in narrative 22 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SLBP is the central post-transcriptional regulator of replication-dependent histone mRNAs, coupling histone biogenesis to S-phase progression through roles in 3'-end processing, nuclear export, translation, and mRNA degradation. Its RNA-binding domain is intrinsically disordered and folds upon phosphorylation-dependent binding to the conserved 3' stem-loop of histone mRNAs, where a phosphothreonine in the TPNK motif increases RNA affinity by stabilizing a hydrogen-bonding network and slowing the off-rate, while SLBP binding remodels the pre-mRNA to facilitate U7 snRNP recruitment for endonucleolytic cleavage (PMID:22439849, PMID:25379382, PMID:16982637). SLBP accompanies processed histone mRNA to the cytoplasm for translation, recruiting the translation activator SLIP1—a MIF4G-domain homodimer that bridges SLBP to eIF3—through a phosphorylation-gated heterodimer assembly mechanism (PMID:23804756, PMID:23286197, PMID:19155325). SLBP abundance is itself cell-cycle-regulated by at least three ubiquitin ligase systems—CRL4(WDR23) activating ubiquitylation during S phase, SCF–cyclin F targeting SLBP for G2 degradation via a CY motif, and FEM1-family CUL2 ligases mediating degradation in other phases—with Pin1/PP2A promoting SLBP dissociation from histone mRNA and subsequent polyubiquitination at the S/G2 boundary (PMID:27203182, PMID:27773672, PMID:28118078, PMID:22907757). Haploinsufficiency of SLBP in Wolf-Hirschhorn syndrome patient cells causes defective chromatin assembly, delayed S-phase progression, and increased DNA damage sensitivity (PMID:22328085).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 2003 High

    Establishing that SLBP is the sole cell-cycle-regulated processing factor answered how histone pre-mRNA 3'-end cleavage is restricted to S phase: phosphorylation of T60/T62 triggers proteasomal degradation at the S/G2 boundary, and recombinant SLBP restores processing activity to G1 or G2 nuclear extracts that otherwise lack it.

    Evidence Mass spectrometry of purified SLBP, phosphosite mutagenesis, nuclear extract reconstitution in human cells

    PMID:12588979

    Open questions at the time
    • Identity of the E3 ligase recognizing the T60/T62 phosphodegron was unknown
    • Whether additional post-translational modifications govern SLBP stability remained open
  2. 2004 High

    Structural characterization of the RNA-binding domain resolved how an intrinsically disordered protein achieves specific RNA recognition: NMR showed the N-terminal/RBD regions are disordered in isolation but adopt nascent helical structure, and phosphorylation of C-terminal serines in the RPD is required for proper domain folding and processing activity.

    Evidence NMR spectroscopy (Drosophila SLBP), 31P NMR, circular dichroism, in vitro processing assays, phosphomimetic mutagenesis

    PMID:15260482 PMID:15260483

    Open questions at the time
    • High-resolution structure of the SLBP–RNA complex was not yet available
    • Whether phosphorylation-coupled folding operates identically in human SLBP was untested
  3. 2004 High

    Demonstrating that SLBP remains on histone mRNA on polyribosomes established its post-processing cytoplasmic role: SLBP co-immunoprecipitates with histone mRNA from polyribosome fractions and relocalizes to the nucleus upon replication inhibition while remaining functional for RNA binding.

    Evidence Polyribosome fractionation and RNA co-immunoprecipitation in mammalian cells

    PMID:15358832

    Open questions at the time
    • The mechanism by which SLBP stimulates translation was unclear
    • How SLBP relocalizes to the nucleus upon replication stress was undefined
  4. 2005 Medium

    Linking SLBP to DNA replication efficiency showed that its role extends beyond RNA metabolism: RNAi depletion of SLBP slows S-phase progression, rescued by RNAi-resistant SLBP re-expression.

    Evidence RNAi knockdown with genetic rescue, double-thymidine block, flow cytometry in human cells

    PMID:15916543

    Open questions at the time
    • Whether the replication defect is due to histone shortage versus other SLBP functions was not distinguished
    • Single lab study
  5. 2006 Medium

    RNA structure probing revealed that SLBP binding remodels the histone pre-mRNA substrate to expose the histone downstream element for U7 snRNP, explaining how SLBP catalytically facilitates the processing reaction beyond simple stem-loop recognition.

    Evidence Chemical/enzymatic RNA structure probing and EMSA in vitro

    PMID:16982637

    Open questions at the time
    • No reconstitution of full processing complex with U7 snRNP was performed
    • Single lab, in vitro only
  6. 2009 High

    Identification of SLBP's requirement for histone mRNA nuclear export added a new function: SLBP knockdown causes nuclear retention of properly processed histone mRNA, linking SLBP to mRNP export beyond its known processing and translation roles.

    Evidence RNAi knockdown, RNA FISH, cell fractionation in U2OS cells

    PMID:19155325

    Open questions at the time
    • The export receptor or adaptor recruited by SLBP was not identified
    • Whether SLBP directly contacts export machinery was untested
  7. 2012 High

    NMR mapping of the human SLBP RNA-binding domain defined two discrete RNA-contact sites and showed that TPNK phosphothreonine increases RNA affinity by slowing the off-rate, providing the first detailed kinetic and structural mechanism for phosphorylation-enhanced RNA binding.

    Evidence NMR spectroscopy and kinetic binding assays on human SLBP

    PMID:22439849

    Open questions at the time
    • A full atomic-resolution structure of the SLBP–stem-loop complex was still lacking
    • Contributions of individual contact residues to in vivo function were not tested
  8. 2012 High

    Discovery that Pin1 and PP2A coordinately regulate SLBP dissociation from histone mRNA and subsequent degradation resolved how SLBP is removed from mRNPs at the end of S phase: Pin1 isomerizes phospho-Ser/Thr-Pro motifs to promote PP2A-mediated dephosphorylation of TPNK, releasing SLBP from RNA, and simultaneously stimulates polyubiquitination via the Ser20/Ser23 phosphodegron.

    Evidence In vitro dephosphorylation reconstitution (Pin1 + PP2A), ubiquitination assays, RNA stability measurements

    PMID:22907757

    Open questions at the time
    • The kinase(s) responsible for Ser20/Ser23 phosphorylation were not identified
    • Order of events (RNA release versus ubiquitination) in living cells was not resolved
  9. 2012 Medium

    Analysis of Wolf-Hirschhorn syndrome patient cells demonstrated that SLBP haploinsufficiency causes defective chromatin assembly (reduced histone-chromatin association, increased MNase sensitivity), delayed S-phase progression, and DNA damage hypersensitivity, establishing a human disease connection.

    Evidence Patient-derived cell lines with 4p deletions, flow cytometry, MNase sensitivity, histone fractionation

    PMID:22328085

    Open questions at the time
    • Other genes deleted in the 4p region could contribute to observed phenotypes
    • No rescue experiment with exogenous SLBP was performed
  10. 2013 High

    Crystal structures of the SLIP1–SLBP translation activation complex and SLIP1–eIF3g/DBP5 interactions revealed the molecular bridge between SLBP and the translation machinery: SLIP1 is a MIF4G-domain homodimer that binds SLBP and recruits eIF3 through a conserved SLIP1-binding motif, with phosphorylation-gated heterodimer/heterotetramer switching controlling complex assembly on histone mRNA.

    Evidence X-ray crystallography (2.5 Å and 3.25 Å), pull-down assays, analytical ultracentrifugation, RNA EMSA, in vivo histone mRNA quantification

    PMID:23286197 PMID:23804756

    Open questions at the time
    • No structure of the full ternary SLBP–SLIP1–eIF3 complex on RNA
    • Contribution of DBP5 to histone mRNA translation in vivo was not assessed
  11. 2016 High

    Identification of two distinct ubiquitin ligase pathways targeting SLBP resolved the enzymology of its cell-cycle-regulated turnover: SCF–cyclin F degrades SLBP in G2 via a CY motif (with failure causing toxic H2A.X overexpression), while CRL4(WDR23) ubiquitylates SLBP non-degradatively to activate its processing function during S phase.

    Evidence Reciprocal Co-IP, CY motif mutagenesis, in vitro ubiquitylation reconstitution, polyribosome profiling, genetic knockdown

    PMID:27203182 PMID:27773672

    Open questions at the time
    • The ubiquitin chain topology deposited by CRL4(WDR23) for activation was not determined
    • How CRL4(WDR23)-mediated ubiquitylation mechanistically activates processing was unknown
  12. 2017 High

    Discovery that FEM1A/B/C CUL2 ubiquitin ligases target SLBP via N-terminal degrons completed the degradation circuit: combined mutation of cyclin F and FEM1 interaction sites eliminates SLBP oscillation, demonstrating that multiple E3 ligases cooperate across different cell-cycle phases to ensure tight SLBP turnover.

    Evidence Co-immunoprecipitation, combined degron mutagenesis, conservation validated in C. elegans and Drosophila

    PMID:28118078

    Open questions at the time
    • Relative contribution of each FEM1 family member in specific cell-cycle phases was not delineated
    • Structural basis of FEM1–SLBP degron recognition was not determined
  13. 2019 Medium

    Zebrafish Slbp loss-of-function mutants showed that SLBP is required for the proliferation-to-differentiation transition in the developing CNS, broadening its biological role beyond histone supply to cell-fate decisions.

    Evidence Forward genetic screen, genetic mapping, RNA-seq, in situ hybridization in zebrafish

    PMID:30695021

    Open questions at the time
    • Whether the differentiation defect is a direct consequence of altered chromatin or secondary to cell-cycle arrest was not resolved
    • Single model organism
  14. 2021 Medium

    Identification of a Drosophila SLBP domain outside the RPD that is essential for histone gene transcription and mRNA deposition in oocytes revealed a separable transcriptional role for SLBP, distinct from its canonical 3'-end processing function.

    Evidence Deletion mutant analysis, in situ hybridization, genetic rescue in Drosophila oogenesis

    PMID:33408246

    Open questions at the time
    • Whether this transcriptional role is conserved in vertebrates is unknown
    • The transcription factor or chromatin target of this domain was not identified
  15. 2025 Medium

    Discovery that Drosophila maternal histone mRNAs are uniquely polyadenylated with truncated stem-loops—requiring SLBP but not U7 snRNP—revealed a noncanonical SLBP-dependent processing pathway for maternally deposited histones.

    Evidence Genetic mutant analysis of SLBP and U7 snRNP in Drosophila, RNA-seq, polysome analysis

    PMID:40239992

    Open questions at the time
    • Whether noncanonical polyadenylated histone mRNAs exist in vertebrate oocytes is unknown
    • The endonuclease generating the truncated stem-loop was not identified

Open questions

Synthesis pass · forward-looking unresolved questions
  • A high-resolution structure of the full human SLBP–stem-loop RNA complex, the mechanism by which CRL4(WDR23) ubiquitylation activates SLBP processing function, the identity of the nuclear export receptor recruited by SLBP, and whether SLBP's transcriptional role (seen in Drosophila oogenesis) is conserved in mammals remain unresolved.
  • No atomic-resolution structure of full-length SLBP bound to histone mRNA stem-loop
  • Mechanism of activating ubiquitylation by CRL4(WDR23) is undefined
  • Nuclear export pathway for SLBP-histone mRNP is unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 8 GO:0140098 catalytic activity, acting on RNA 4
Localization
GO:0005634 nucleus 3 GO:0005829 cytosol 2
Pathway
R-HSA-8953854 Metabolism of RNA 8 R-HSA-1640170 Cell Cycle 5 R-HSA-392499 Metabolism of proteins 4 R-HSA-4839726 Chromatin organization 1
Partners
CCNFEIF3GFEM1AFEM1BFEM1CPIN1SLIP1WDR23
Complex memberships
Histone mRNPSLBP-SLIP1 translation activation complex

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2003 SLBP is degraded at the end of S phase via phosphorylation of two threonines (T60 and T62 in the TTP sequence and a consensus cyclin binding site at aa 99-104). Mass spectrometry of purified SLBP from late S-phase cells confirmed phosphorylation of these residues, which triggers proteasomal degradation. Mutation of these residues stabilizes SLBP in G2/M. Nuclear extracts from G1 and G2 cells are deficient in histone pre-mRNA processing, which is restored by recombinant SLBP, demonstrating SLBP is the only cell cycle-regulated factor required for histone pre-mRNA processing. Mass spectrometry of purified SLBP, site-directed mutagenesis, nuclear extract reconstitution assay Molecular and cellular biology High 12588979
2009 SLBP knockdown by RNAi in U2OS cells reveals that SLBP is required for nuclear export of histone mRNA. Reduced SLBP results in nuclear retention of properly processed histone mRNA, accumulation of cells in S phase, and loss of rapid histone mRNA degradation upon DNA replication inhibition, demonstrating a role for SLBP in histone mRNA export. RNAi knockdown, RNA FISH, cell fractionation, fluorescence microscopy RNA (New York, N.Y.) High 19155325
2016 Cyclin F (F-box protein, substrate recognition subunit of SCF complex) is identified as the G2 ubiquitin ligase for SLBP. SLBP interacts with cyclin F via an atypical CY motif; mutation of this motif prevents SLBP degradation in G2. Stable SLBP causes increased loading of H2AFX mRNA onto polyribosomes, elevated H2A.X, persistent γH2A.X signaling, and apoptosis upon genotoxic stress in G2. Co-immunoprecipitation, CY motif mutagenesis, polyribosome profiling, stable SLBP mutant expression Molecular cell High 27773672
2016 The CUL4 E3 ubiquitin ligase complex CRL4(WDR23) binds and ubiquitylates SLBP in vitro and in vivo. This ubiquitylation activates SLBP function in histone mRNA 3' end processing without affecting SLBP protein levels; loss of CRL4(WDR23) activity results in histone depletion and impaired DNA replication. In vitro ubiquitylation assay, co-immunoprecipitation, genetic knockdown, histone mRNA processing assay Molecular cell High 27203182
2012 The prolyl isomerase Pin1 regulates SLBP degradation and histone mRNA stability. Pin1 promotes SLBP polyubiquitination via the Ser20/Ser23 phosphodegron. Pin1 together with PP2A can dephosphorylate phospho-Thr in the conserved TPNK sequence of the SLBP RNA binding domain in vitro, dissociating SLBP from the histone mRNA hairpin. Pin1 knockdown increases histone mRNA stability and accumulates SLBP in the nucleus. siRNA knockdown, in vitro dephosphorylation assay (Pin1 + PP2A), ubiquitination assay, RNA stability assay Molecular and cellular biology High 22907757
2013 Crystal structure of zebrafish SLIP1 bound to the translation-activation domain of SLBP determined at 2.5 Å. SLIP1 is a MIF4G-like homodimer that connects SLBP to translation initiation. A SLIP1-binding motif (SBM) was identified in eIF3g and DBP5; pull-down assays and crystal structure of SLIP1-DBP5 SBM confirmed these interactions. SLIP1 homodimerization and SBM-binding residues are conserved in CTIF. X-ray crystallography, pull-down assays, structure determination at 2.5 Å and 3.25 Å Nucleic acids research High 23804756
2004 SLBP is a component of the histone mRNP on polyribosomes. Anti-SLBP immunoprecipitation co-precipitates histone mRNA from polyribosomes. When DNA replication is inhibited, histone mRNAs are rapidly degraded but SLBP remains constant and relocalizes to the nucleus, remaining active in RNA binding and histone pre-mRNA processing. Polyribosome fractionation, co-immunoprecipitation of RNA, subcellular fractionation Nucleic acids research High 15358832
2012 SLBP has two binding sites for the histone mRNA hairpin within its RNA binding domain: a helix-turn-helix motif (Glu129–Val158) recognizing unpaired uridines in the stem-loop, and a second site (Arg180–Pro200) recognizing the second G-C base pair. Threonine phosphorylation at the conserved TPNK sequence increases SLBP affinity for histone mRNA by slowing off-rate; the adjacent proline acts as a hinge regulating the second binding site. NMR spectroscopy, kinetic binding assays, phosphorylation analysis Biochemistry High 22439849
2013 Assembly of the SLIP1-SLBP complex on histone mRNA requires sequential binding: phosphorylated SLBP binds the histone mRNA stem-loop first, then SLIP1 associates to form an active ternary complex. Unphosphorylated SLBP and SLIP1 form an inactive 2:2 heterotetramer that cannot bind histone mRNA; phosphorylation at Thr171 promotes dissociation to the active heterodimer. A single-point SLIP1 mutant near the homodimer interface abolished SLBP interaction and reduced histone mRNA in vivo. Analytical ultracentrifugation, baculovirus phosphorylated SLBP, alanine scanning mutagenesis, RNA EMSA, in vivo histone mRNA quantification Biochemistry High 23286197
2006 SLBP binding to the 3'-UTR of histone pre-mRNA H4-12 induces structural rearrangements that open the HDE hairpin, making it accessible for U7 snRNA anchoring. RNA structure probing and EMSA demonstrated that SLBP facilitates U7 snRNP binding to the pre-mRNA. RNA structure probing (chemical/enzymatic), EMSA Nucleic acids research Medium 16982637
2004 The N-terminal domain of Drosophila SLBP is intrinsically disordered with nascent helical structure at physiological conditions. NMR characterization shows four regions with helical propensity but no well-defined tertiary fold in the absence of RNA, consistent with a disordered-to-ordered transition upon RNA binding. NMR spectroscopy (15N, 13C labeling), circular dichroism Biochemistry High 15260482
2004 Phosphorylation of C-terminal serines in the SLBP RNA binding and processing domain (RPD) is necessary for histone pre-mRNA processing in Drosophila. Both serine phosphorylation and RNA binding are required for proper folding of the RPD; the electrostatic effect of phosphorylation can be partially mimicked by serine-to-glutamate mutations. 31P NMR, circular dichroism, in vitro processing assay, phosphomimetic mutagenesis Biochemistry High 15260483
2017 FEM1A, FEM1B, and FEM1C (VHL-box CUL2-RING E3 ubiquitin ligase substrate recognition subunits) interact with SLBP via distinct degrons in SLBP's N-terminus and mediate SLBP degradation. An SLBP mutant unable to interact with cyclin F plus all three FEM1 proteins is expressed at higher levels and does not oscillate during the cell cycle. FEM1-SLBP ortholog interactions are conserved in C. elegans and D. melanogaster. Co-immunoprecipitation, mutagenesis (combined degron mutants), C. elegans genetic depletion of FEM1 Cell cycle (Georgetown, Tex.) High 28118078
2005 SLBP is required for efficient DNA replication. RNAi-mediated removal of SLBP increases the number of cells in S phase and causes slow progression through S phase upon release from a double-thymidine block. Re-expression of an RNAi-resistant SLBP restores proper S-phase progression, demonstrating the effect is specifically due to SLBP loss. RNAi, rescue with RNAi-resistant SLBP, double-thymidine block cell cycle analysis Biochemical Society transactions Medium 15916543
2012 Haploinsufficiency of SLBP in Wolf-Hirschhorn syndrome patient-derived cells causes delayed S-to-M phase progression, reduced DNA replication, altered chromatin assembly (reduced histone-chromatin association, elevated soluble chaperone-bound histone H3, increased MNase sensitivity), and increased camptothecin sensitivity. Patient-derived cell lines with variable 4p deletions, flow cytometry, MNase sensitivity assay, histone-chromatin fractionation Human molecular genetics Medium 22328085
2022 53BP1 acts as a molecular scaffold for NDPK-mediated phosphorylation of ATP-citrate lyase (ACLY), enhancing ACLY activity, global histone acetylation, and transcriptome-wide gene expression changes. SLBP expression is dependent on 53BP1-ACLY-controlled acetylation at the SLBP promoter, placing SLBP downstream in a 53BP1-ACLY-SLBP axis that coordinates replication-dependent histone biogenesis. Co-immunoprecipitation, ChIP, ACLY activity assay, transcriptomic analysis Nucleic acids research Medium 35037047
2014 31P NMR demonstrates that a phosphothreonine in the RNA binding domain of SLBP exhibits torsional strain and participates in a hydrogen-bonding network; phosphorylation at this residue promotes assembly of the SLBP-RNA complex through phosphate-coupled folding of an intrinsically disordered region. 31P NMR spectroscopy FEBS open bio Medium 25379382
2019 Loss of Slbp function in zebrafish (eisspalte mutants) causes failure of cells to transition from proliferation to differentiation, retinal coloboma, midline axon guidance deficits, and altered expression of neuronal differentiation and axon guidance genes. Cells throughout the CNS remain in the cell cycle at stages when post-mitotic differentiation normally occurs. Forward genetic screen, genetic mapping, RNAseq, in situ hybridization PloS one Medium 30695021
2021 In Drosophila stage 10B oocytes, a region of SLBP outside the mRNA-processing domain (overlapping a putative nuclear localization sequence) is essential for histone mRNA deposition into the oocyte and for histone gene transcription. A 10-amino-acid deletion or NLS mutations prevent histone gene transcription in stage 10B without affecting S-phase processing function. SLBP deletion mutant analysis, in situ hybridization, genetic rescue in Drosophila Journal of cell science Medium 33408246
2004 Yeast three-hybrid system analysis of human SLBP identified critical residues in the RNA binding domain required for histone hairpin recognition. Both the core RBD and flanking N- and C-terminal domains contribute to RNA discrimination. Yeast three-hybrid system, negative and positive selection screens FEBS letters Low 14706861
2017 Nickel and cadmium compounds deplete SLBP mRNA and protein levels, resulting in increased polyadenylated canonical histone H3.1 mRNA. SLBP protein depletion is reversed by proteasome inhibition. Rescue of SLBP mRNA and protein by epigenetic modifiers suggests nickel acts through epigenetic mechanisms to reduce SLBP expression. Western blot, qRT-PCR, proteasome inhibitor treatment, epigenetic modifier rescue PloS one Low 28306745
2025 In Drosophila, maternal histone mRNAs are uniquely polyadenylated and have a truncated 3' stem-loop. This noncanonical 3'-end processing requires SLBP but not U7 snRNP during oogenesis. Maternal histone transcripts undergo cytoplasmic poly(A) elongation by Wisp for stabilization and translation, and their translation is activated upon loss of embryonic linker histone dBigH1. Genetic mutant analysis (SLBP and U7 snRNP mutants in Drosophila), RNA sequencing, polysome analysis, in situ hybridization Nucleic acids research Medium 40239992

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Heparin-binding protein (HBP/CAP37): a missing link in neutrophil-evoked alteration of vascular permeability. Nature medicine 283 11590435
2008 Neutrophil primary granule proteins HBP and HNP1-3 boost bacterial phagocytosis by human and murine macrophages. The Journal of clinical investigation 181 18787642
2015 INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. Science (New York, N.Y.) 126 26068852
1991 HBP-1a and HBP-1b: leucine zipper-type transcription factors of wheat. The EMBO journal 121 2026143
2007 Limited tolerance towards folded elements during secretion of the autotransporter Hbp. Molecular microbiology 100 17302825
2003 Phosphorylation of stem-loop binding protein (SLBP) on two threonines triggers degradation of SLBP, the sole cell cycle-regulated factor required for regulation of histone mRNA processing, at the end of S phase. Molecular and cellular biology 89 12588979
2009 Knockdown of SLBP results in nuclear retention of histone mRNA. RNA (New York, N.Y.) 80 19155325
2005 Neutrophil-derived heparin-binding protein (HBP/CAP37) deposited on endothelium enhances monocyte arrest under flow conditions. Journal of immunology (Baltimore, Md. : 1950) 71 15879141
2004 Atorvastatin reduces CD68, FABP4, and HBP expression in oxLDL-treated human macrophages. Biochemical and biophysical research communications 68 15110783
2009 Leukotriene B4-induced changes in vascular permeability are mediated by neutrophil release of heparin-binding protein (HBP/CAP37/azurocidin). FASEB journal : official publication of the Federation of American Societies for Experimental Biology 66 19151333
2020 First characterization of glucose flux through the hexosamine biosynthesis pathway (HBP) in ex vivo mouse heart. The Journal of biological chemistry 65 31915250
1996 Characterization of recombinant human HBP/CAP37/azurocidin, a pleiotropic mediator of inflammation-enhancing LPS-induced cytokine release from monocytes. FEBS letters 63 8706818
2016 Cyclin F-Mediated Degradation of SLBP Limits H2A.X Accumulation and Apoptosis upon Genotoxic Stress in G2. Molecular cell 61 27773672
2017 MircroRNA-19a promotes vascular inflammation and foam cell formation by targeting HBP-1 in atherogenesis. Scientific reports 60 28935967
2017 Heparin-Binding Protein (HBP): A Causative Marker and Potential Target for Heparin Treatment of Human Sepsis-Induced Acute Kidney Injury. Shock (Augusta, Ga.) 55 28319494
1997 Structure of HBP, a multifunctional protein with a serine proteinase fold. Nature structural biology 52 9095193
1999 Discovery of a putative heme-binding protein family (SOUL/HBP) by two-tissue suppression subtractive hybridization and database searches. Brain research. Molecular brain research 49 10640688
2010 A conserved aromatic residue in the autochaperone domain of the autotransporter Hbp is critical for initiation of outer membrane translocation. The Journal of biological chemistry 48 20923769
2022 Bladder Cancer-Derived Small Extracellular Vesicles Promote Tumor Angiogenesis by Inducing HBP-Related Metabolic Reprogramming and SerRS O-GlcNAcylation in Endothelial Cells. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 47 36045101
2004 SLBP is associated with histone mRNA on polyribosomes as a component of the histone mRNP. Nucleic acids research 46 15358832
2001 STAM/EAST/Hbp adapter proteins--integrators of signalling pathways. FEBS letters 43 11728436
2012 The prolyl isomerase Pin1 targets stem-loop binding protein (SLBP) to dissociate the SLBP-histone mRNA complex linking histone mRNA decay with SLBP ubiquitination. Molecular and cellular biology 41 22907757
2017 Heparin-binding protein (HBP) improves prediction of sepsis-related acute kidney injury. Annals of intensive care 40 29047023
2012 Characterizing the functional consequences of haploinsufficiency of NELF-A (WHSC2) and SLBP identifies novel cellular phenotypes in Wolf-Hirschhorn syndrome. Human molecular genetics 39 22328085
2020 GFAT1/HBP/O-GlcNAcylation Axis Regulates β-Catenin Activity to Promote Pancreatic Cancer Aggressiveness. BioMed research international 35 32149084
1999 Characterization of the biosynthesis, processing, and sorting of human HBP/CAP37/azurocidin. Journal of leukocyte biology 35 10534120
1994 The HBP-1 family of wheat basic/leucine zipper proteins interacts with overlapping cis-acting hexamer motifs of plant histone genes. The Journal of biological chemistry 35 8144592
2011 Aberrant activation of Wnt/β-catenin signaling pathway contributes to the sequential progression of DMBA-induced HBP carcinomas. Oral oncology 34 21924667
2001 Hrs and hbp: possible regulators of endocytosis and exocytosis. Biochemical and biophysical research communications 34 11243842
1989 Multiplicity of the DNA-binding protein HBP-1 specific to the conserved hexameric sequence ACGTCA in various plant gene promoters. FEBS letters 34 2680601
2013 Structural and biochemical studies of SLIP1-SLBP identify DBP5 and eIF3g as SLIP1-binding proteins. Nucleic acids research 32 23804756
2002 Basic residues in azurocidin/HBP contribute to both heparin binding and antimicrobial activity. The Journal of biological chemistry 32 11994286
2017 FEM1 proteins are ancient regulators of SLBP degradation. Cell cycle (Georgetown, Tex.) 31 28118078
1997 High-density lipoprotein-binding protein (HBP)/vigilin is expressed in human atherosclerotic lesions and colocalizes with apolipoprotein E. Arteriosclerosis, thrombosis, and vascular biology 31 9409201
2012 Interaction of the histone mRNA hairpin with stem-loop binding protein (SLBP) and regulation of the SLBP-RNA complex by phosphorylation and proline isomerization. Biochemistry 30 22439849
1989 Wheat nuclear protein HBP-1 binds to the hexameric sequence in the promoter of various plant genes. Nucleic acids research 29 2602142
2019 Knocking down miR-384 promotes growth and metastasis of osteosarcoma MG63 cells by targeting SLBP. Artificial cells, nanomedicine, and biotechnology 27 31007083
2016 CRL4(WDR23)-Mediated SLBP Ubiquitylation Ensures Histone Supply during DNA Replication. Molecular cell 26 27203182
2005 Expression of an RNAi-resistant SLBP restores proper S-phase progression. Biochemical Society transactions 26 15916543
2008 Usefulness of competitive inhibitors of protein binding for improving the pharmacokinetics of 186Re-MAG3-conjugated bisphosphonate (186Re-MAG3-HBP), an agent for treatment of painful bone metastases. European journal of nuclear medicine and molecular imaging 24 18709369
2020 Repeated measures of Heparin-binding protein (HBP) and procalcitonin during septic shock: biomarker kinetics and association with cardiovascular organ dysfunction. Intensive care medicine experimental 23 32910266
2021 GFAT2 mediates cardiac hypertrophy through HBP-O-GlcNAcylation-Akt pathway. iScience 21 34934932
2006 The first structure from the SOUL/HBP family of heme-binding proteins, murine P22HBP. The Journal of biological chemistry 21 16905545
1998 Conserved Ser residues in the basic region of the bZIP-type transcription factor HBP-1a(17): importance in DNA binding and possible targets for phosphorylation. Plant molecular biology 21 9484468
2021 Suppression of the HBP Function Increases Pancreatic Cancer Cell Sensitivity to a Pan-RAS Inhibitor. Cells 20 33670598
2017 HBP induces the expression of monocyte chemoattractant protein-1 via the FAK/PI3K/AKT and p38 MAPK/NF-κB pathways in vascular endothelial cells. Cellular signalling 20 29288710
2004 The N-terminal domain of the Drosophila histone mRNA binding protein, SLBP, is intrinsically disordered with nascent helical structure. Biochemistry 19 15260482
1998 Atomic resolution structure of human HBP/CAP37/azurocidin. Acta crystallographica. Section D, Biological crystallography 19 9761855
2021 DRAIC promotes growth of breast cancer by sponging miR-432-5p to upregulate SLBP. Cancer gene therapy 18 34645975
2013 Heparin-binding protein (HBP) in critically ill patients with influenza A(H1N1) infection. Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases 18 23402373
2023 HBP/O-GlcNAcylation Metabolic Axis Regulates Bone Resorption Outcome. Journal of dental research 17 36749069
2021 FOXA2 inhibits doxorubicin-induced apoptosis via transcriptionally activating HBP rate-limiting enzyme GFPT1 in HCC cells. Journal of physiology and biochemistry 17 34291417
2013 Heparin-binding protein (HBP): an early marker of respiratory failure after trauma? Acta anaesthesiologica Scandinavica 17 23320546
2005 Effect of buffalo seminal plasma heparin binding protein (HBP) on freezability and in vitro fertility of buffalo cauda spermatozoa. Animal reproduction science 16 16143473
2001 Purification of the autotransporter protein Hbp of Escherichia coli. FEMS microbiology letters 16 11728729
1997 Gene regulation of HBP 23 by metalloporphyrins and protoporphyrin IX in liver and hepatocyte cultures. Biochemical and biophysical research communications 16 9070867
2018 Photothermal/pH Dual-Responsive Drug Delivery System of Amino-Terminated HBP-Modified rGO and the Chemo-Photothermal Therapy on Tumor Cells. Nanoscale research letters 15 30470923
2008 The conserved extension of the Hbp autotransporter signal peptide does not determine targeting pathway specificity. Biochemical and biophysical research communications 15 18258183
2014 Heparin-binding protein (HBP/CAP37) - a link to endothelin-1 in endotoxemia-induced pulmonary oedema? Acta anaesthesiologica Scandinavica 14 24611481
2002 Low resolution solution structure of the Apo form of Escherichia coli haemoglobin protease Hbp. Journal of molecular biology 14 11827485
1996 HALF-1, a bZIP-type protein, interacting with the wheat transcription factor HBP-1a contains a novel transcriptional activation domain. Genes to cells : devoted to molecular & cellular mechanisms 14 9078369
2004 Electrostatic contribution of serine phosphorylation to the Drosophila SLBP--histone mRNA complex. Biochemistry 13 15260483
1990 99mTc-neoglycoalbumin (NGA)-binding to human hepatic binding protein (HBP) in vitro. British journal of clinical pharmacology 13 2306412
2017 Nickel and cadmium-induced SLBP depletion: A potential pathway to metal mediated cellular transformation. PloS one 12 28306745
2013 Assembly of the SLIP1-SLBP complex on histone mRNA requires heterodimerization and sequential binding of SLBP followed by SLIP1. Biochemistry 12 23286197
2010 Role of domains within the autotransporter Hbp/Tsh. Acta crystallographica. Section D, Biological crystallography 12 21123869
2006 Binding of human SLBP on the 3'-UTR of histone precursor H4-12 mRNA induces structural rearrangements that enable U7 snRNA anchoring. Nucleic acids research 11 16982637
2002 Micrometastases from HBP malignancies and metastatic cancer. Journal of hepato-biliary-pancreatic surgery 11 12541044
1999 Structure and function of the N-linked glycans of HBP/CAP37/azurocidin: crystal structure determination and biological characterization of nonglycosylated HBP. Protein science : a publication of the Protein Society 11 10548047
2023 Protective Effect and Mechanism of Xbp1s Regulating HBP/O-GlcNAcylation through GFAT1 on Brain Injury after SAH. Biomedicines 10 37238930
2022 miR-27b-3p Improved High Glucose-Induced Spermatogenic Cell Damage via Regulating Gfpt1/HBP Signaling. European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes 10 34986481
2022 Heparin-Binding Protein (HBP), Neutrophil Gelatinase-Associated Lipocalin (NGAL) and S100 Calcium-Binding Protein B (S100B) Can Confirm Bacterial Meningitis and Inform Adequate Antibiotic Treatment. Antibiotics (Basel, Switzerland) 10 35740230
2021 Heparin-binding protein (HBP) worsens the severity of pancreatic necrosis via up-regulated M1 macrophages activation in acute pancreatitis mouse models. Bioengineered 10 34895060
2004 Critical residues for RNA discrimination of the histone hairpin binding protein (HBP) investigated by the yeast three-hybrid system. FEBS letters 10 14706861
1997 Dissection of the wheat transcription factor HBP-1a(17) reveals a modular structure for the activation domain. Molecular & general genetics : MGG 10 9065688
1993 Mouse heparin binding protein-44 (HBP-44) associates with brushin, a high-molecular-weight glycoprotein antigen common to the kidney and teratocarcinomas. Journal of biochemistry 10 8282724
2007 Seminal plasma non-heparin binding proteins (NHBP) reduce the cryoinjury to buffalo cauda epididymal spermatozoa induced by heparin binding proteins (HBP). Animal reproduction science 9 17433582
2025 Accuracy of blood heparin-binding protein (HBP) for diagnosis bacteremia in patients with sepsis. Scientific reports 8 39962104
2022 Clinical value of serum sTREM-1 and HBP levels in combination with traditional inflammatory markers in diagnosing hospital-acquired pneumonia in elderly. BMC infectious diseases 8 36195852
2011 New 3-, 8-disubstituted pyrazolo[5,1-c][1,2,4]benzotriazines useful for studying the interaction with the HBp-3 area (hydrogen bond point area) in the benzodiazepine site on the γ-aminobutyric acid type A (GABAA) receptor. Bioorganic & medicinal chemistry 8 21530274
1993 Chromosomal locations of the genes for histones and a histone gene-binding protein family HBP-1 in common wheat. Plant molecular biology 8 8343598
2025 Combinational Analysis of Metabolomic and O-GlcNAcylation Omics Reveals the HBP Metabolic Regulation of Chemoresistance via GFPT1/NR3C1 O-GlcNAcylation/GPX4 Axis. Research (Washington, D.C.) 7 40740652
2021 A region of SLBP outside the mRNA-processing domain is essential for deposition of histone mRNA into the Drosophila egg. Journal of cell science 7 33408246
2019 Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits. PloS one 7 30695021
2014 Contribution of protein phosphorylation to binding-induced folding of the SLBP-histone mRNA complex probed by phosphorus-31 NMR. FEBS open bio 7 25379382
1999 Crystallographic characterization of a stress-induced multifunctional protein, rat HBP-23. Journal of structural biology 7 10329492
1995 Developmental and tissue-specific regulation of the gene for the wheat basic/leucine zipper protein HBP-1a(17) in transgenic Arabidopsis plants. Molecular & general genetics : MGG 7 7476857
2022 53BP1-ACLY-SLBP-coordinated activation of replication-dependent histone biogenesis maintains genomic integrity. Nucleic acids research 6 35037047
2010 Identifying the functional part of heparin-binding protein (HBP) as a monocyte stimulator and the novel role of monocytes as HBP producers. Innate immunity 6 20083497
2020 Insights into Mobile Genetic Elements of the Biocide-Degrading Bacterium Pseudomonas nitroreducens HBP-1. Genes 5 32806781
2019 Molecular characterization and expression patterns of stem-loop binding protein (SLBP) genes in protogynous hermaphroditic grouper, Epinephelus coioides. Gene 5 30910559
1991 Quantification of human hepatic binding protein (HBP) via 99mTc-galactosyl-neoglycoalbumin (NGA) liver scintigraphy. Wiener klinische Wochenschrift 5 1926872
1986 Enhancement of affinity to receptors in the esterified glucocorticoid, hydrocortisone 17-butyrate 21-propionate (HBP), in the rat liver. Biochemical pharmacology 5 3718538
2024 Multi-omics reveals the molecular mechanism of the combined toxic effects of PFOA and 4-HBP exposure in MCF-7 cells and the key player: mTORC1. Environment international 4 38815467
2019 [Diagnostic value of HBP, PCT combined with APACHE Ⅱ score respectively in ventilator-associated pneumonia]. Zhonghua yi xue za zhi 4 31216814
2025 Maternal histone mRNAs are uniquely processed through polyadenylation in a Stem-Loop Binding Protein (SLBP) dependent manner. Nucleic acids research 3 40239992
2017 Data for the generation of RNA spatiotemporal distributions and interpretation of Chk1 and SLBP protein depletion phenotypes during Drosophila embryogenesis. Data in brief 3 28560279
2013 Glucan HBP-A increase type II collagen expression of chondrocytes in vitro and tissue engineered cartilage in vivo. Chinese journal of integrative medicine 3 23860800
2025 The HBP Pathway Inhibitor FR054 Enhances Temozolomide Sensitivity in Glioblastoma Cells by Promoting Ferroptosis and Inhibiting O-GlcNAcylation. CNS neuroscience & therapeutics 2 40772310
2023 AZU1 (HBP/CAP37) and PRKCG (PKC-gamma) may be candidate genes affecting the severity of acute mountain sickness. BMC medical genomics 2 36803152