Affinage

SH3PXD2A

SH3 and PX domain-containing protein 2A · UniProt Q5TCZ1

Round 2 corrected
Length
1133 aa
Mass
125.3 kDa
Annotated
2026-04-28
130 papers in source corpus 11 papers cited in narrative 11 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SH3PXD2A (Tks5/Fish) is a multi-domain scaffold protein that functions as a critical organizer of invadopodia and podosomes, coupling Src tyrosine kinase signaling to invasive cell migration, extracellular matrix degradation, and cell–cell fusion. Identified as a Src substrate with a PX domain and five SH3 domains, Tks5 is recruited to invadopodium precursors via PX domain binding to PI(3,4)P₂, where it stabilizes nascent structures rather than initiating them, and scaffolds NADPH oxidase 1 (Nox1) for localized ROS production and ADAM family metalloproteinases for matrix remodeling (PMID:12615925, PMID:24206842, PMID:19755710). Tks5 is required for tumor growth and vascularization in vivo, for osteoclast fusion downstream of PI3K/Src, and its expression in rheumatoid arthritis T cells—driven by altered glycolytic metabolism—confers tissue-invasive capacity (PMID:18417249, PMID:22584907, PMID:28737753). Homozygous disruption of Sh3pxd2a in mice causes neonatal lethality with complete cleft of the secondary palate (PMID:25259869).

Mechanistic history

Synthesis pass · year-by-year structured walk · 9 steps
  1. 1998 High

    Identifying Tks5 as a novel Src substrate with five SH3 domains and a PX domain established it as a potential signaling scaffold linking tyrosine kinase signaling to actin cytoskeleton remodeling.

    Evidence Anti-phosphotyrosine cDNA library screen with in vivo phosphorylation assays in Src-transformed and growth factor-treated fibroblasts

    PMID:9687503

    Open questions at the time
    • Cellular function of the protein was unknown
    • PX domain lipid specificity was uncharacterized
    • SH3 domain interaction partners were unidentified
  2. 2003 High

    Demonstrating that the PX domain binds PI(3,4)P₂ and mediates relocalization to podosomes in Src-transformed cells, and that SH3 domains interact with ADAM metalloproteinases, revealed how Tks5 is spatially targeted and linked to proteolytic effectors.

    Evidence PX domain lipid-binding assays, GFP-fusion imaging, phage display screen, co-immunoprecipitation and co-localization in Src-transformed fibroblasts

    PMID:12615925

    Open questions at the time
    • Whether Tks5 is functionally required for podosome formation was untested
    • In vivo relevance was unknown
    • Other SH3 domain partners beyond ADAMs were uncharacterized
  3. 2005 High

    Loss-of-function experiments established that Tks5 is required for podosome formation, gelatin degradation, and Matrigel invasion, transforming its status from a Src substrate to a necessary functional component of the invasive machinery.

    Evidence siRNA knockdown with invasion and degradation assays in Src-transformed and cancer cell lines; immunolocalization in human tumors

    PMID:15710328

    Open questions at the time
    • Whether Tks5 is needed for initiation versus stabilization of podosomes was unclear
    • In vivo tumor growth contribution was untested
    • Non-cancer physiological roles were unknown
  4. 2008 High

    In vivo tumor xenograft studies showed Tks5 knockdown reduces tumor growth and vascularization but not metastatic seeding, positioning Tks5-dependent podosomes as mediators of tumor growth and angiogenesis rather than extravasation.

    Evidence Stable shRNA knockdown; subcutaneous and tail vein injection models in mice with histological analysis

    PMID:18417249

    Open questions at the time
    • Mechanism linking podosomes to vascularization was unresolved
    • Whether effects are cell-autonomous was not fully addressed
  5. 2009 High

    Reconstitution experiments revealed Tks5 as a p47phox-related organizer of Nox1/Nox3 NADPH oxidases, recruiting Nox1 to invadopodia for localized ROS production, thereby connecting the scaffold to redox signaling at invasive structures.

    Evidence Reconstituted Nox activity assays, co-immunoprecipitation with NoxA1, immunolocalization at invadopodia, siRNA knockdown

    PMID:19755710

    Open questions at the time
    • How ROS production mechanistically promotes invadopodium maturation was unresolved
    • Relative contributions of Nox-organizing versus ADAM-recruiting functions were unclear
  6. 2012 High

    Demonstrating that Tks5 is induced during osteoclastogenesis and is required for circumferential podosome organization and multinucleation—dependent on Src-mediated tyrosine phosphorylation—extended its physiological role beyond cancer to bone homeostasis and cell fusion.

    Evidence shRNA knockdown during osteoclastogenesis, Src−/− osteoclast analysis, phosphorylation assays, co-culture fusion assays, live imaging

    PMID:22584907

    Open questions at the time
    • Identity of phosphorylated tyrosine residues critical for function was not fully mapped
    • Whether Tks5 mediates other physiological fusion events was unknown
  7. 2013 High

    High-resolution live imaging resolved the temporal order of invadopodium assembly, showing Tks5 arrives after the initial cortactin/N-WASP/actin core and is dispensable for precursor initiation but essential for PI(3,4)P₂-dependent stabilization and maturation.

    Evidence Spatiotemporal live-cell imaging with PI(3,4)P₂ biosensors, siRNA knockdown of Tks5 and SHIP2

    PMID:24206842

    Open questions at the time
    • Structural basis of PX domain–PI(3,4)P₂ interaction at invadopodia was unresolved
    • Downstream effectors recruited by stabilized Tks5 remain incompletely defined
  8. 2014 High

    Gene-trap disruption of Sh3pxd2a in mice established an essential developmental role, with homozygous loss causing neonatal lethality and complete secondary palate clefting, and revealed a PX-domain-lacking isoform (Tks5β) regulated by the proteasome.

    Evidence Gene-trap mouse model with phenotypic analysis; 5'RACE; Western blot; proteasome inhibitor treatment

    PMID:25259869

    Open questions at the time
    • Whether palate defect is podosome-dependent was untested
    • Function of the Tks5β isoform in vivo was not determined
    • Whether SH3PXD2A mutations cause human craniofacial disorders was unknown
  9. 2017 High

    Discovery that altered glycolytic metabolism in rheumatoid arthritis T cells upregulates Tks5 to drive tissue-invasive podosome formation linked metabolic reprogramming to the invasive scaffold, revealing a non-cancer pathological context for Tks5 function.

    Evidence Gene expression analysis in RA T cells; metabolic interventions (pyruvate, fatty acid synthesis inhibitors); in vivo arthritis model; Tks5 knockdown/overexpression

    PMID:28737753

    Open questions at the time
    • Transcriptional or post-transcriptional mechanism connecting lipid droplets to Tks5 expression was not fully elucidated
    • Whether Tks5 contributes to other autoimmune tissue-invasive phenotypes was untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the structural basis of Tks5 scaffold assembly at invadopodia, the specific tyrosine residues controlling its function, the in vivo role of the Tks5β isoform, and whether SH3PXD2A mutations contribute to human craniofacial or skeletal disease.
  • No high-resolution structure of Tks5 or its domain complexes
  • Functional significance of individual SH3 domains beyond the fifth is poorly defined
  • Human genetic disease association not established

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 5 GO:0008092 cytoskeletal protein binding 2 GO:0008289 lipid binding 2
Localization
GO:0005886 plasma membrane 4 GO:0005829 cytosol 1
Pathway
R-HSA-162582 Signal Transduction 3 R-HSA-1474244 Extracellular matrix organization 2 R-HSA-1266738 Developmental Biology 1
Partners
ADAM12ADAM15ADAM19NOX1NOXA1SHIP2SRC

Evidence

Reading pass · 11 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1998 Fish (SH3PXD2A/Tks5) was identified as a novel Src tyrosine kinase substrate containing five SH3 domains and a PX domain; it is tyrosine-phosphorylated in Src-transformed fibroblasts and in normal cells following growth factor treatment or cytochalasin D treatment, implicating it in actin cytoskeleton signaling downstream of tyrosine kinases. Anti-phosphotyrosine antibody screening of cDNA expression libraries; in vivo phosphorylation assays in Src-transformed and growth factor-treated cells The EMBO journal High 9687503
2003 The PX domain of Fish (Tks5) binds 3-phosphorylated phosphatidylinositols (PI3P and PI(3,4)P2); full-length Fish is largely cytoplasmic in normal fibroblasts but dramatically relocalizes to actin-rich podosomes in Src-transformed cells, with the PX domain necessary and sufficient for this relocalization. Fish interacts with ADAM family members (ADAM12, 15, 19) via its fifth SH3 domain, and ADAM12 co-localizes with Fish at podosomes. PX domain lipid-binding assays; GFP-fusion localization imaging; phage display screen with SH3 domain; co-immunoprecipitation and co-localization in Src-transformed cells The Journal of biological chemistry High 12615925
2005 Tks5/Fish is required for podosome formation and function in Src-transformed cells; knockdown of Tks5/Fish abolishes podosome formation, gelatin degradation, and matrigel invasion. Co-expression of Tks5/Fish and Src in epithelial cells is sufficient to induce podosomes. Tks5/Fish expression is detected in podosomes of invasive cancer cells and in human breast cancer and melanoma samples. siRNA knockdown with invasion and gelatin degradation assays; co-expression experiments; immunolocalization in cancer cell lines and tumor samples Cancer cell High 15710328
2008 Tks5 is required for tumor growth in vivo; Src-transformed NIH-3T3 cells with stable Tks5 knockdown form significantly smaller subcutaneous tumors with reduced vascularization. Tail vein injection produced smaller lung metastases but no difference in lesion number, suggesting podosomes mediate tumor growth and angiogenesis but not extravasation. Stable shRNA knockdown; subcutaneous and tail vein injection tumor models in mice; histological analysis of vascularization European journal of cell biology High 18417249
2009 Tks5 functions as a p47(phox)-related organizer that selectively supports NADPH oxidase 1 (Nox1) and Nox3 activity (but not Nox2 or Nox4) in reconstituted cellular systems, interacting with the NoxA1 activator protein through an SH3 domain-mediated interaction. Tks5 recruits Nox1 to invadopodia, linking localized ROS production to invadopodium formation. Reconstituted Nox activity assays; Co-IP with NoxA1; immunolocalization at invadopodia; siRNA knockdown of Tks4/Tks5 Science signaling High 19755710
2012 Tks5 is required for osteoclast fusion downstream of PI3K and Src; Tks5 expression is induced during osteoclastogenesis and is necessary for circumferential podosome formation and cell-cell fusion without affecting differentiation. Tyrosine phosphorylation of Tks5 by Src is required for proper podosome organization and multinucleation. Tks5 also mediates circumferential invadopodia formation in melanoma cells enabling melanoma-osteoclast hybrid cell formation. shRNA knockdown during osteoclastogenesis; Src−/− osteoclast analysis; phosphorylation assays; co-culture fusion assays; live imaging The Journal of cell biology High 22584907
2013 Tks5 is not required for invadopodium precursor initiation but is essential for precursor stabilization; the PX domain of Tks5 interacts with PI(3,4)P2 locally accumulated at the invadopodium core, and Tks5 arrives after the initial cortactin/N-WASP/cofilin/actin core. SHIP2 (a 5'-inositol phosphatase) arrives coincident with PI(3,4)P2 accumulation and regulates mature invadopodium formation and matrix degradation. High-resolution spatiotemporal live-cell imaging; siRNA knockdown of Tks5 and SHIP2; SHIP2 overexpression; PI(3,4)P2 and PI(3,4,5)P3 biosensor imaging Current biology : CB High 24206842
2014 Homozygous disruption of sh3pxd2a in mice by gene-trapping results in neonatal death and complete cleft of the secondary palate. Gene-trap mice lack only the highest molecular weight Tks5 isoform, revealing the existence of a second alternative isoform Tks5β, which lacks the PX domain and instead has a unique N-terminal sequence encoded by a newly discovered exon 6β. Tks5β is a Src substrate and its levels are regulated by the proteasome. Gene-trap mouse mutant analysis; 5'RACE on mouse fibroblasts; protein analysis by Western blot; Src phosphorylation assays; proteasome inhibitor treatment PloS one High 25259869
2017 TKS5 expression is upregulated in rheumatoid arthritis (RA) T cells, enabling them to form tissue-invasive podosome-like membrane structures. This upregulation is driven by reduced glycolytic flux (low ATP and pyruvate), which triggers fatty acid biosynthesis and lipid droplet formation. Restoring pyruvate or inhibiting fatty acid synthesis corrects T cell tissue invasiveness in vivo. Gene expression analysis in RA T cells; metabolic manipulation (pyruvate restoration, fatty acid synthesis inhibition); in vivo mouse arthritis model; TKS5 knockdown/overexpression Nature immunology High 28737753
1997 SH3PXD2A (as KIAA0303) was identified and partially sequenced from human brain cDNA libraries as a large protein-coding gene, providing the first sequence information for the locus. cDNA library screening and in vitro transcription/translation DNA research Low 9455477
2006 Phage display screening of the complete human SH3 proteome identified SH3 domains of Fish (SH3PXD2A) among those with preferred binding to ADAM15, providing an unbiased confirmation that Fish SH3 domains mediate specific nanomolar-affinity interactions with ADAM family members. Phage display of 296 human SH3 domains against ADAM15 ligand EMBO reports Medium 16374509

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2010 Network organization of the human autophagy system. Nature 1286 20562859
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2009 Spermatogenesis in fish. General and comparative endocrinology 715 19348807
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2008 Regulation of oocyte maturation in fish. Development, growth & differentiation 495 18482399
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2006 The interferon system of teleost fish. Fish & shellfish immunology 416 15939626
2002 Ammonia toxicity in fish. Marine pollution bulletin 406 12398363
2013 Protein interaction network of the mammalian Hippo pathway reveals mechanisms of kinase-phosphatase interactions. Science signaling 383 24255178
2009 Perspectives on fish gonadotropins and their receptors. General and comparative endocrinology 343 19686749
2005 The adaptor protein Tks5/Fish is required for podosome formation and function, and for the protease-driven invasion of cancer cells. Cancer cell 304 15710328
2017 Genome-wide CRISPR screen identifies HNRNPL as a prostate cancer dependency regulating RNA splicing. Proceedings of the National Academy of Sciences of the United States of America 282 28611215
2003 Regulation of fish gonadotropins. International review of cytology 275 12696592
2004 Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation. Nature biotechnology 266 15146197
2006 Effects of microcystins on fish. Environmental toxicology and chemistry 255 16494227
2011 The interleukins of fish. Developmental and comparative immunology 225 21605591
2014 Antimicrobial peptides from fish. Pharmaceuticals (Basel, Switzerland) 215 24594555
2001 Paradigms of growth in fish. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 212 11399452
2016 An organelle-specific protein landscape identifies novel diseases and molecular mechanisms. Nature communications 211 27173435
2003 The adaptor protein fish associates with members of the ADAMs family and localizes to podosomes of Src-transformed cells. The Journal of biological chemistry 206 12615925
2020 Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions. Nature cell biology 194 32203420
2011 Chemokines in teleost fish species. Developmental and comparative immunology 184 21414348
2003 A Cortactin-CD2-associated protein (CD2AP) complex provides a novel link between epidermal growth factor receptor endocytosis and the actin cytoskeleton. The Journal of biological chemistry 178 12672817
2011 Selected reaction monitoring mass spectrometry reveals the dynamics of signaling through the GRB2 adaptor. Nature biotechnology 172 21706016
2006 CRF and stress in fish. General and comparative endocrinology 167 16403502
2003 'Antifreeze' glycoproteins from polar fish. European journal of biochemistry 163 12653993
2005 Endotoxin recognition: in fish or not in fish? FEBS letters 157 16297386
2016 Fish Immunoglobulins. Biology 152 27879632
1998 A new method for isolating tyrosine kinase substrates used to identify fish, an SH3 and PX domain-containing protein, and Src substrate. The EMBO journal 148 9687503
2009 Ubiquitin-mediated proteolysis of HuR by heat shock. The EMBO journal 142 19322201
2019 Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms. Nature cell biology 137 31871319
2006 Antibody repertoire development in cartilaginous fish. Developmental and comparative immunology 137 16146649
2005 Genomics of fish cytokines. Comparative biochemistry and physiology. Part D, Genomics & proteomics 137 20483237
2006 Cytotoxic activities of fish leucocytes. Fish & shellfish immunology 136 15939625
1998 Lymphocyte development in fish and amphibians. Immunological reviews 136 9914914
2017 RNA-binding activity of TRIM25 is mediated by its PRY/SPRY domain and is required for ubiquitination. BMC biology 135 29117863
2005 DNA vaccines for aquacultured fish. Revue scientifique et technique (International Office of Epizootics) 133 16110889
2013 Tks5 and SHIP2 regulate invadopodium maturation, but not initiation, in breast carcinoma cells. Current biology : CB 132 24206842
2015 T Cells in Fish. Biology 129 26426066
1994 Olfaction and gustation in fish: an overview. Acta physiologica Scandinavica 121 7839864
2017 Metabolic control of the scaffold protein TKS5 in tissue-invasive, proinflammatory T cells. Nature immunology 118 28737753
2014 Fish allergens at a glance: variable allergenicity of parvalbumins, the major fish allergens. Frontiers in immunology 112 24795722
1994 On the scoring of FISH-"painted" chromosome-type exchange aberrations. Mutation research 112 7513815
2010 Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Molecular medicine (Cambridge, Mass.) 108 20379614
2006 Identification of preferred protein interactions by phage-display of the human Src homology-3 proteome. EMBO reports 99 16374509
2012 Which fish should I eat? Perspectives influencing fish consumption choices. Environmental health perspectives 98 22534056
2008 Pigments, patterns, and fish behavior. Zebrafish 98 19133828
2008 A role for the podosome/invadopodia scaffold protein Tks5 in tumor growth in vivo. European journal of cell biology 96 18417249
2017 FISH-ing for captured contacts: towards reconciling FISH and 3C. Nature methods 93 28604723
2013 Turbo FISH: a method for rapid single molecule RNA FISH. PloS one 92 24066168
2016 Transcriptomic responses in the fish intestine. Developmental and comparative immunology 89 26995769
2016 Closing the loop: 3C versus DNA FISH. Genome biology 89 27760553
2012 Tks5-dependent formation of circumferential podosomes/invadopodia mediates cell-cell fusion. The Journal of cell biology 88 22584907
2012 Immunity to fish rhabdoviruses. Viruses 87 22355456
2009 Novel p47(phox)-related organizers regulate localized NADPH oxidase 1 (Nox1) activity. Science signaling 86 19755710
2011 Fish melanocortin system. European journal of pharmacology 83 21208603
2017 An Adenosine Receptor for Olfaction in Fish. Current biology : CB 80 28502661
2014 Gonadal development in fish. Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation 77 25034975
2011 Heterogeneity of macrophage activation in fish. Developmental and comparative immunology 76 21414343
2022 Toxic Effects of Cadmium on Fish. Toxics 74 36287901
2021 Demethylation of Methylmercury in Bird, Fish, and Earthworm. Environmental science & technology 73 33476127
1975 Olfaction in fish. Progress in neurobiology 70 830087
1997 Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro. DNA research : an international journal for rapid publication of reports on genes and genomes 68 9455477
2012 Maternal immunity in fish. Developmental and comparative immunology 65 22387589
2008 Thyrotropin in teleost fish. General and comparative endocrinology 64 19135445
2014 Strategies and hurdles using DNA vaccines to fish. Veterinary research 62 24552235
2006 Fish intake and serum fatty acid profiles from freshwater fish. The American journal of clinical nutrition 62 17158409
2004 Cytotoxicity assays with fish cells as an alternative to the acute lethality test with fish. Alternatives to laboratory animals : ATLA 61 15651922
1993 Allergic reactions to fish. Clinical reviews in allergy 59 8221508
1990 Metamorphosis and fish vision. Journal of neurobiology 59 2258720
2005 Update on viral vaccines for fish. Developments in biologicals 58 15962473
1997 Aeromonas species in fish, fish-eggs, shrimp and freshwater. International journal of food microbiology 58 9029253
1962 Blood coagulation in fish. The American journal of physiology 57 22103034
2004 Fluorescence in situ Hybridization (FISH). Current protocols in cell biology 56 18228455
2002 Fluorescence in situ hybridization (FISH) in diagnostic and investigative neuropathology. Brain pathology (Zurich, Switzerland) 54 11770903
2009 Cytoarchitecture of a cichlid fish telencephalon. Brain, behavior and evolution 53 19729898
1996 Experimental chemical carcinogenesis in fish. Toxicologic pathology 53 8923683
2018 Adipogenesis in fish. The Journal of experimental biology 50 29514876
2015 Bioactive components in fish venoms. Toxins 50 25941767
2020 Protein changes as robust signatures of fish chronic stress: a proteomics approach to fish welfare research. BMC genomics 49 32306896
2018 Nongenomic cortisol signaling in fish. General and comparative endocrinology 49 29673844
1997 FISH probes for mouse chromosome identification. Genomics 49 9339359
2008 Towards brain repair: Insights from teleost fish. Seminars in cell & developmental biology 48 19111625
2018 Fish, Fish Oils and Cardioprotection: Promise or Fish Tale? International journal of molecular sciences 46 30469489
1993 Arginine vasotocin and fish osmoregulation. Fish physiology and biochemistry 43 24202476
2011 Taxonomy of bacterial fish pathogens. Veterinary research 42 21314902
2021 Dietary Fish, Fish Nutrients, and Immune Function: A Review. Frontiers in nutrition 41 33553231
2013 Functional aspects of fish lymphocytes. Developmental and comparative immunology 41 23707785
1997 FISH technology in chromosome and genome research. BioEssays : news and reviews in molecular, cellular and developmental biology 41 9008419
2012 Orexin system in teleost fish. Vitamins and hormones 39 22640622
2006 Multicolor fluorescence in situ hybridization (FISH) applied to FISH-banding. Cytogenetic and genome research 39 16954660
2003 Formation of chromosome aberrations: insights from FISH. Mutation research 38 14644331
2000 Embryonic and genetic manipulation in fish. Cell research 38 10765980
1991 Transgenesis in fish. Experientia 38 1915772
2001 Allergens from fish and egg. Allergy 37 11298006
2020 Association of lncRNA SH3PXD2A-AS1 with preeclampsia and its function in invasion and migration of placental trophoblast cells. Cell death & disease 36 32719429
2020 Advances in Detecting Ciguatoxins in Fish. Toxins 36 32752046
2001 Somatostatins and their receptors in fish. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 36 11399490
2013 Up-regulated expression and aberrant DNA methylation of LEP and SH3PXD2A in pre-eclampsia. PloS one 35 23544093
2006 Ribonucleases and angiogenins from fish. The Journal of biological chemistry 32 16861230
1995 Fish oil, atherogenesis, and thrombogenesis. Annals of the New York Academy of Sciences 31 7535028
2014 Genetic disruption of the sh3pxd2a gene reveals an essential role in mouse development and the existence of a novel isoform of tks5. PloS one 30 25259869
2015 Antibody Repertoires in Fish. Results and problems in cell differentiation 29 26537383
2021 Integration of Nutrient Sensing in Fish Hypothalamus. Frontiers in neuroscience 28 33716662
2006 Multiplex-FISH (M-FISH): technique, developments and applications. Cytogenetic and genome research 28 16954655
2002 Recombinant fish parvalbumins: Candidates for diagnosis and treatment of fish allergy. Allergy 28 12144564
2002 Mineralization patterns in elasmobranch fish. Microscopy research and technique 28 12430168
1992 Using fluorescence in situ hybridization (FISH) in genome mapping. Trends in biotechnology 28 1367928
2016 Enzymes in Fish and Seafood Processing. Frontiers in bioengineering and biotechnology 27 27458583
2014 Impacts of moonlight on fish reproduction. Marine genomics 26 24393605
2007 Heme, an iron supply for vibrios pathogenic for fish. Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine 26 17206385
2013 RNA detection in situ with FISH-STICs. RNA (New York, N.Y.) 25 24345395
2018 The inner ear proteome of fish. The FEBS journal 24 30521091
2003 CA125 and UQCRFS1 FISH studies of ovarian carcinoma. Gynecologic oncology 24 12821338
1990 Transgenic fish. Trends in biotechnology 24 1366668
2020 Commercial fish ELISA kits have a limited capacity to detect different fish species and their products. Journal of the science of food and agriculture 23 32356561
2011 Nevus versus melanoma: to FISH, or not to FISH. Advances in anatomic pathology 23 21490440
2007 Interaction between the ADAM12 and SH3MD1 genes may confer susceptibility to late-onset Alzheimer's disease. American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics 23 17440933
2024 Immunotoxicity of microplastics in fish. Fish & shellfish immunology 21 38735599
2020 FISH and chips: a review of microfluidic platforms for FISH analysis. Medical microbiology and immunology 20 31965296
2000 FISH-microdissection (FISH-MD) analysis of complex chromosome rearrangements. Cytogenetics and cell genetics 20 10773683
2020 Kleptoprotein bioluminescence: Parapriacanthus fish obtain luciferase from ostracod prey. Science advances 19 31934625
2020 Megalocytiviruses in ornamental fish: A review. Veterinary world 19 33363355
2000 Polyunsaturated fatty acid (fish or evening primrose oil) for schizophrenia. The Cochrane database of systematic reviews 19 10796622
2004 Multi-color FISH techniques. Current protocols in cell biology 18 18228456