Affinage

SEPSECS

O-phosphoseryl-tRNA(Sec) selenium transferase · UniProt Q9HD40

Round 2 corrected
Length
501 aa
Mass
55.7 kDa
Annotated
2026-04-28
130 papers in source corpus 11 papers cited in narrative 10 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

SEPSECS is the pyridoxal phosphate-dependent enzyme that catalyzes the final step of selenocysteine (Sec) biosynthesis, converting O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) using selenophosphate as the selenium donor (PMID:17142313, PMID:17194211). The crystal structure of the human enzyme reveals a homotetrameric architecture in which tRNA(Sec) binding—through its distinctive 13-bp acceptor-TΨC arm—induces an active-site conformational change that orients the phosphoseryl moiety for catalysis, thereby enforcing strict substrate discrimination against free phosphoserine (PMID:19608919). SEPSECS resides in a multiprotein complex with SECp43, SEPHS1, and SEPHS2; SECp43 promotes nucleocytoplasmic shuttling of SEPSECS and is required for full tRNA(Sec) methylation and selenoprotein expression (PMID:16230358, PMID:28414460). Loss-of-function mutations in SEPSECS abolish selenoprotein synthesis and cause autosomal-recessive progressive cerebellocerebral atrophy (PCCA) and related encephalopathies (PMID:20920667, PMID:26115735).

Mechanistic history

Synthesis pass · year-by-year structured walk · 8 steps
  1. 2000 High

    Identification of SEPSECS as the protein component of the tRNA(Ser)Sec ribonucleoprotein complex resolved the molecular identity of the SLA/LP autoantigen in autoimmune hepatitis and placed the protein within the selenocysteine biosynthesis machinery.

    Evidence cDNA expression library immunoscreening; immunoprecipitation of tRNA(Ser)Sec from HeLa cell extracts with eluted antibodies

    PMID:10801173 PMID:10931155

    Open questions at the time
    • Enzymatic function of the protein not yet demonstrated
    • Relationship between autoantigenicity and catalytic role unknown
  2. 2005 High

    Demonstrating that SEPSECS forms a complex with SECp43 and tRNA(Sec), and that SECp43 controls SEPSECS nuclear translocation and tRNA(Sec) methylation, established the regulatory context in which SEPSECS operates before its catalytic role was formally proven.

    Evidence RNA interference knockdown; co-immunoprecipitation; subcellular fractionation in mammalian cells

    PMID:16230358

    Open questions at the time
    • Mechanism by which SECp43 promotes nuclear import of SEPSECS not defined
    • Relative contributions of cytoplasmic vs. nuclear SEPSECS pools to selenoprotein synthesis unknown
  3. 2006 High

    In vitro reconstitution and in vivo complementation proved that SEPSECS is the eukaryotic/archaeal Sec synthase, resolving a long-standing gap in the selenocysteine biosynthetic pathway.

    Evidence Purified recombinant enzyme assay converting Sep-tRNA(Sec) to Sec-tRNA(Sec); complementation of E. coli SelA deletion

    PMID:17142313

    Open questions at the time
    • Structural basis for substrate recognition not yet determined
    • Selenium donor specificity not fully resolved
  4. 2007 High

    Defining the obligatory substrate requirements—O-phosphoseryl-tRNA(Sec) rather than free phosphoserine, and selenophosphate as the selenium donor—established the two-component specificity of the SEPSECS reaction.

    Evidence Full reconstitution with purified tRNA, PSTK, SEPSECS, and selenophosphate synthetase; substrate specificity assays

    PMID:17194211

    Open questions at the time
    • Kinetic parameters and rate-limiting step not characterized
    • Whether selenophosphate is directly transferred or proceeds via intermediate unknown
  5. 2009 High

    The crystal structure of the SEPSECS–tRNA(Sec) complex revealed the homotetrameric architecture, the PLP-dependent mechanism, and the tRNA-induced conformational change that enforces substrate discrimination, providing an atomic-level explanation for the tRNA-dependent catalysis.

    Evidence X-ray crystallography of human SEPSECS with tRNA(Sec), phosphoserine, and thiophosphate; complementary in vivo and in vitro enzyme assays

    PMID:19608919

    Open questions at the time
    • No structure of the selenophosphate-bound intermediate captured
    • Dynamics of the conformational switch not resolved at the time-resolved level
  6. 2010 High

    Identification of homozygous loss-of-function SEPSECS mutations as the cause of progressive cerebellocerebral atrophy proved the enzyme is essential for selenoprotein synthesis and normal brain development in humans.

    Evidence Homozygosity mapping and sequencing in consanguineous families; clinical phenotyping

    PMID:20920667

    Open questions at the time
    • Specific selenoproteins whose loss drives cerebellar neurodegeneration not identified
    • No animal model recapitulating PCCA phenotype described at this point
  7. 2015 High

    Characterization of hypomorphic SEPSECS mutations extended the genotype-phenotype spectrum, showing that partial enzyme activity leads to reduced but not absent selenoprotein synthesis with elevated oxidative damage, linking disease severity to residual catalytic capacity.

    Evidence Whole-exome sequencing; bacterial complementation assay; mass spectrometry and protein oxidation analysis of patient brain tissue

    PMID:26115735

    Open questions at the time
    • Quantitative relationship between residual SEPSECS activity and selenoprotein output not determined
    • Contribution of oxidative damage versus specific selenoprotein loss to neuropathology not dissected
  8. 2017 Medium

    BRET and co-immunoprecipitation data showed that SEPSECS forms oligomeric assemblies with SEPHS1 and SEPHS2 in cells, extending the interaction network beyond the previously described SECp43 partnership and suggesting a selenocysteine biosynthesis supramolecular complex.

    Evidence BRET assay and co-immunoprecipitation in mammalian cells

    PMID:28414460

    Open questions at the time
    • Functional consequence of SEPSECS–SEPHS1/2 interaction on catalytic efficiency not measured
    • Stoichiometry and architecture of the full complex not resolved
    • Findings from a single laboratory

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key open questions include the structure of a catalytic intermediate with selenophosphate bound, the identity of the specific selenoproteins whose loss drives cerebellar neurodegeneration, and whether the SEPSECS–SEPHS–SECp43 supramolecular complex channels selenophosphate directly to the active site.
  • No selenophosphate-bound catalytic intermediate structure
  • Selenoprotein-specific contributions to PCCA neuropathology unresolved
  • Substrate channeling within the multiprotein complex not tested

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 3 GO:0016874 ligase activity 3
Localization
GO:0005634 nucleus 1 GO:0005829 cytosol 1
Pathway
R-HSA-392499 Metabolism of proteins 4
Partners
SEPHS1SEPHS2TRNAU1AP
Complex memberships
Selenocysteine biosynthesis complex (SEPSECS–SECp43–SEPHS1–SEPHS2)

Evidence

Reading pass · 10 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2006 SEPSECS (then annotated as SLA/LP) catalyzes the tRNA-dependent conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) in eukaryotes and archaea. Purified recombinant SepSecS converts Sep-tRNA(Sec) into Sec-tRNA(Sec) in vitro in the presence of sodium selenite and selenophosphate synthetase. Human and archaeal SepSecS genes complement an E. coli SelA deletion strain in vivo, establishing SepSecS as the eukaryotic/archaeal Sec synthase. In vitro enzyme assay with purified recombinant protein; in vivo complementation of E. coli SelA deletion strain Proceedings of the National Academy of Sciences of the United States of America High 17142313
2007 SEPSECS (SecS/soluble liver antigen) requires O-phosphoseryl-tRNA([Ser]Sec) as its obligatory substrate (not free phosphoserine or seryl-tRNA), and requires selenophosphate as the selenium donor, to generate selenocysteyl-tRNA([Ser]Sec). This establishes the two-component substrate requirement for SecS activity. In vitro biochemical reconstitution with purified components (tRNA, PSTK, SEPSECS, selenophosphate synthetase); substrate specificity assays PLoS biology High 17194211
2009 Crystal structure of human SepSecS (SLA/LP) in complex with tRNA(Sec), phosphoserine, and thiophosphate reveals: (1) SepSecS functions as a tetramer; (2) two tRNA(Sec) molecules bind per tetramer through their 13-bp acceptor-TΨC arm, which has a fold distinct from canonical tRNAs; (3) tRNA binding induces a conformational change in the active site that correctly orients phosphoserine covalently attached to tRNA(Sec) but not free phosphoserine; (4) the reaction proceeds via a pyridoxal phosphate-dependent mechanism. X-ray crystallography; in vivo and in vitro enzyme assays; active-site analysis Science (New York, N.Y.) High 19608919
2005 SLA (SEPSECS) forms a multiprotein complex with SECp43 and tRNA([Ser]Sec) in cells. Knockdown of SECp43 reduces tRNA(Sec) wobble-position methylation (Um34) and decreases selenoprotein expression; double knockdown of SECp43 and SLA further reduces selenoprotein expression. SECp43 and SLA each affect the other's binding to tRNA(Sec). SLA is located primarily in the cytoplasm, but co-transfection with SECp43 causes nuclear translocation of SLA, indicating SECp43 promotes nucleocytoplasmic shuttling of SLA. RNA interference knockdown; co-immunoprecipitation; subcellular fractionation/localization; selenoprotein expression assays The Journal of biological chemistry High 16230358
2010 Loss-of-function mutations in SEPSECS cause autosomal-recessive progressive cerebellocerebral atrophy (PCCA) in humans. Both founder mutations (in Iraqi and Moroccan Jewish populations) disrupt the sole route to selenocysteine biosynthesis and thus abolish selenoprotein synthesis, establishing SEPSECS as an essential enzyme in vivo for selenoprotein generation and brain function. Human genetics (homozygosity mapping, sequencing); functional inference from established SEPSECS enzymatic role American journal of human genetics High 20920667
2015 Compound heterozygous SEPSECS mutations (p.Thr325Ser and p.Tyr429*) do not completely abolish enzyme activity but lead to decreased selenoprotein levels and increased oxidative protein damage in patient brain tissue, extending the phenotypic spectrum of SEPSECS deficiency to a progressive encephalopathy with elevated lactate. A bacterial activity assay confirmed that the missense mutation partially impairs but does not eliminate SEPSECS function. Whole-exome sequencing; bacterial complementation activity assay; mass spectrometry; Western blotting; protein oxidation detection Neurology High 26115735
2017 SEPSECS, SECp43, SEPHS1, and SEPHS2 form oligomers in eukaryotic cells. SEPHS2 interacts with SEPSECS and SEPHS1; these interactions were confirmed by bioluminescence resonance energy transfer (BRET) assay and co-immunoprecipitation in mammalian cells. Bioluminescence resonance energy transfer (BRET) assay in mammalian cells; co-immunoprecipitation Biochemistry Medium 28414460
2000 The target antigen of SLA/LP autoantibodies in autoimmune hepatitis is a 422-amino-acid protein (SEPSECS/tRNP(Ser)Sec-associated protein). The protein was identified by cDNA expression library immunoscreening; antibodies eluted from the recombinant protein immunoprecipitate tRNA(Ser)Sec from HeLa cell extracts, demonstrating that the autoantigen is the protein component of the tRNA(Ser)Sec ribonucleoprotein complex. cDNA expression library immunoscreening; inhibition ELISA; immunoprecipitation of tRNA(Ser)Sec from cell extracts with eluted antibodies Clinical and experimental immunology High 10801173 10931155
2009 The native SEPSECS protein in human hepatic cell extracts has an apparent molecular weight of 52 kDa, as determined by immunoaffinity chromatography followed by mass spectrometry identification, confirming the identity of the recombinant antigen with the endogenous protein. Immunoaffinity chromatography; mass spectrometry; monoclonal antibody validation Journal of autoimmunity Medium 19683415
2012 In the chicken brain, selenium supplementation increases SEPSECS mRNA levels and extends SEPSECS mRNA half-life, whereas selenium does not alter SEPSECS mRNA levels in cultured neurons. This indicates that selenium regulates SEPSECS expression post-transcriptionally (mRNA stability) in the avian brain, positioning SEPSECS as part of a selenium homeostasis feedback mechanism. Se supplementation in vivo and in cultured neurons; mRNA stability assays; quantitative RT-PCR PloS one Medium 22536434

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2020 A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature 3411 32353859
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2020 A reference map of the human binary protein interactome. Nature 849 32296183
1999 Hephaestin, a ceruloplasmin homologue implicated in intestinal iron transport, is defective in the sla mouse. Nature genetics 822 9988272
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2011 Global landscape of HIV-human protein complexes. Nature 593 22190034
2020 Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms. Science (New York, N.Y.) 564 33060197
2002 The use of reduced healing times on ITI implants with a sandblasted and acid-etched (SLA) surface: early results from clinical trials on ITI SLA implants. Clinical oral implants research 428 11952734
2005 Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome research 409 16344560
2004 Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation. Nature biotechnology 266 15146197
2007 Biosynthesis of selenocysteine on its tRNA in eukaryotes. PLoS biology 236 17194211
2000 Identification of target antigen for SLA/LP autoantibodies in autoimmune hepatitis. Lancet (London, England) 221 10801173
2016 An organelle-specific protein landscape identifies novel diseases and molecular mechanisms. Nature communications 211 27173435
2015 A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning. eLife 198 26673895
2017 Humoral Reactivity of Renal Transplant-Waitlisted Patients to Cells From GGTA1/CMAH/B4GalNT2, and SLA Class I Knockout Pigs. Transplantation 190 28114170
2006 RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea. Proceedings of the National Academy of Sciences of the United States of America 155 17142313
2008 Molecular genetics of the swine major histocompatibility complex, the SLA complex. Developmental and comparative immunology 146 18760302
2017 Let's talk about Secs: Sec61, Sec62 and Sec63 in signal transduction, oncology and personalized medicine. Signal transduction and targeted therapy 137 29263911
2002 Establishment of standardised SLA/LP immunoassays: specificity for autoimmune hepatitis, worldwide occurrence, and clinical characteristics. Gut 122 12117891
2015 Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes. Molecular systems biology 120 25609649
2009 The human SepSecS-tRNASec complex reveals the mechanism of selenocysteine formation. Science (New York, N.Y.) 120 19608919
2010 Mutations disrupting selenocysteine formation cause progressive cerebello-cerebral atrophy. American journal of human genetics 113 20920667
2000 Isolation and characterization of cDNA encoding the antigenic protein of the human tRNP(Ser)Sec complex recognized by autoantibodies from patients withtype-1 autoimmune hepatitis. Clinical and experimental immunology 111 10931155
2017 Genetics and genotype-phenotype correlations in early onset epileptic encephalopathy with burst suppression. Annals of neurology 105 28133863
1994 Evidence that activation of human T cells by porcine endothelium involves direct recognition of porcine SLA and costimulation by porcine ligands for LFA-1 and CD2. Transplantation 96 7912457
2020 Kinase Interaction Network Expands Functional and Disease Roles of Human Kinases. Molecular cell 88 32707033
2013 The SOX2-interactome in brain cancer cells identifies the requirement of MSI2 and USP9X for the growth of brain tumor cells. PloS one 85 23667531
2011 Enhancing DNA immunization by targeting ASFV antigens to SLA-II bearing cells. Vaccine 79 21679736
2005 Nomenclature for factors of the SLA class-I system, 2004. Tissue antigens 77 15713212
2018 A protein-protein interaction map of the TNF-induced NF-κB signal transduction pathway. Scientific data 74 30561431
2008 The biocompatibility of SLA-treated titanium implants. Biomedical materials (Bristol, England) 73 18458368
2017 SECs (Sinusoidal Endothelial Cells), Liver Microenvironment, and Fibrosis. BioMed research international 66 28293634
2013 Osteoblastic and osteoclastic differentiation on SLA and hydrophilic modified SLA titanium surfaces. Clinical oral implants research 66 23560589
2013 AFF1 is a ubiquitous P-TEFb partner to enable Tat extraction of P-TEFb from 7SK snRNP and formation of SECs for HIV transactivation. Proceedings of the National Academy of Sciences of the United States of America 66 24367103
2005 Evidence for direct roles of two additional factors, SECp43 and soluble liver antigen, in the selenoprotein synthesis machinery. The Journal of biological chemistry 66 16230358
2022 Scalable multiplex co-fractionation/mass spectrometry platform for accelerated protein interactome discovery. Nature communications 65 35831314
2010 A combination of functional polymorphisms in the CASP8, MMP1, IL10 and SEPS1 genes affects risk of non-small cell lung cancer. Lung cancer (Amsterdam, Netherlands) 65 20471133
1995 Directed integration of the physical and genetic linkage maps of swine chromosome 7 reveals that the SLA spans the centromere. Genome research 61 8593613
2001 Soluble liver antigen: isolation of a 35-kd recombinant protein (SLA-p35) specifically recognizing sera from patients with autoimmune hepatitis. Hepatology (Baltimore, Md.) 55 11230739
2015 Selenoprotein biosynthesis defect causes progressive encephalopathy with elevated lactate. Neurology 49 26115735
2005 Rapid assignment of the swine major histocompatibility complex (SLA) class I and II genotypes in Clawn miniature swine using PCR-SSP and PCR-RFLP methods. Xenotransplantation 46 15693842
2002 Fine specificity of autoantibodies to soluble liver antigen and liver/pancreas. Hepatology (Baltimore, Md.) 46 11826415
2014 Anti-SLA/LP alone or in combination with anti-Ro52 and fine specificity of anti-Ro52 antibodies in patients with autoimmune hepatitis. Liver international : official journal of the International Association for the Study of the Liver 42 25113420
2012 Mg ion implantation on SLA-treated titanium surface and its effects on the behavior of mesenchymal stem cell. Materials science & engineering. C, Materials for biological applications 42 23827608
2012 Selenium, selenoprotein genes and Crohn's disease in a case-control population from Auckland, New Zealand. Nutrients 41 23112913
1989 Recognition of xeno-(HLA, SLA) major histocompatibility complex antigens by mouse cytotoxic T cells is not H-2 restricted: a study with transgenic mice. Proceedings of the National Academy of Sciences of the United States of America 40 2783490
2019 HLA Class I-sensitized Renal Transplant Patients Have Antibody Binding to SLA Class I Epitopes. Transplantation 38 30951017
2018 Multifunctional effects of a modification of SLA titanium implant surface with strontium-containing nanostructures on immunoinflammatory and osteogenic cell function. Journal of biomedical materials research. Part A 37 30192064
2021 Immunogenic and efficacious SARS-CoV-2 vaccine based on resistin-trimerized spike antigen SmT1 and SLA archaeosome adjuvant. Scientific reports 36 34750472
2016 Defining the Protein-Protein Interaction Network of the Human Protein Tyrosine Phosphatase Family. Molecular & cellular proteomics : MCP 36 27432908
2019 Triple (GGTA1, CMAH, B2M) modified pigs expressing an SLA class Ilow phenotype-Effects on immune status and susceptibility to human immune responses. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 34 31733031
2002 Anti-soluble liver antigen/liver-pancreas (SLA/LP) antibodies in pediatric patients with autoimmune hepatitis. Autoimmunity 32 12765473
2018 The HIV-1 Tat protein recruits a ubiquitin ligase to reorganize the 7SK snRNP for transcriptional activation. eLife 31 29845934
2017 The ketogenic diet metabolite beta-hydroxybutyrate (β-HB) reduces incidence of seizure-like activity (SLA) in a Katp- and GABAb-dependent manner in a whole-animal Drosophila melanogaster model. Epilepsy research 30 28395176
1995 Polymerase chain reaction based genotyping for characterization of SLA-DQB and SLA-DRB alleles in domestic pigs. Animal genetics 30 7537478
2012 Cationic liposomes containing soluble Leishmania antigens (SLA) plus CpG ODNs induce protection against murine model of leishmaniasis. Parasitology research 29 22223037
2005 Rat sinusoidal liver endothelial cells (SECs) produce pro-fibrotic factors in response to adducts formed from the metabolites of ethanol. Biochemical pharmacology 29 16202982
2015 Using a split luciferase assay (SLA) to measure the kinetics of cell-cell fusion mediated by herpes simplex virus glycoproteins. Methods (San Diego, Calif.) 28 26022509
2009 Swine leukocyte antigen (SLA) diversity in Sinclair and Hanford swine. Developmental and comparative immunology 28 19782700
2014 Immunoliposomes containing Soluble Leishmania Antigens (SLA) as a novel antigen delivery system in murine model of leishmaniasis. Experimental parasitology 26 25246326
1978 Iron deficiency anaemia in newborn sla mice: a genetic defect of placental iron transport. British journal of haematology 26 708646
2013 Effects of Er: YAG laser irradiation on wettability, surface roughness, and biocompatibility of SLA titanium surfaces: an in vitro study. Lasers in medical science 25 23760881
2003 Nucleotide sequencing analysis of the swine 433-kb genomic segment located between the non-classical and classical SLA class I gene clusters. Immunogenetics 25 14673549
2019 The TLR4 agonist adjuvant SLA-SE promotes functional mucosal antibodies against a parenterally delivered ETEC vaccine. NPJ vaccines 24 31149350
2015 PRRSV-infected monocyte-derived dendritic cells express high levels of SLA-DR and CD80/86 but do not stimulate PRRSV-naïve regulatory T cells to proliferate. Veterinary research 24 25990845
2012 Priority in selenium homeostasis involves regulation of SepSecS transcription in the chicken brain. PloS one 24 22536434
2006 Reconstruction of a swine SLA-I protein complex and determination of binding nonameric peptides derived from the foot-and-mouth disease virus. Veterinary immunology and immunopathology 24 16870265
1989 Possible relationships between SLA and porcine reproduction. Reproduction, nutrition, development 24 2513834
1981 Transplantation in miniature swine. X. Evidence for non-SLA-linked immune response gene(s) controlling rejection of SLA-matched kidney allografts. Transplantation 24 6800077
2003 Low frequency of anti-SLA/LP autoantibody in Japanese adult patients with autoimmune liver diseases: analysis with recombinant antigen assay. Journal of autoimmunity 23 12892738
1989 The swine leucocyte antigen (SLA) complex and Sinclair swine cutaneous malignant melanoma. Animal genetics 23 2729673
1999 A selective genetic analysis of the Syracuse high- and low-avoidance (SHA/Bru and SLA/Bru) strains of rats (Rattus norvegicus). Behavioural brain research 22 10595417
2009 SLA/LP/tRNP((Ser)Sec) antigen in autoimmune hepatitis: identification of the native protein in human hepatic cell extract. Journal of autoimmunity 21 19683415
2008 Assignment of the SLA alleles and reproductive potential of selective breeding Duroc pig lines. Xenotransplantation 21 19152667
1996 The SLA RNA gene of Trypanosoma brucei is organized in a tandem array which encodes several small RNAs. Molecular and biochemical parasitology 21 9027749
1992 Mapping of the SLA complex of miniature swine: mapping of the SLA gene complex by pulsed field gel electrophoresis. Mammalian genome : official journal of the International Mammalian Genome Society 21 1543900
2020 Identification of the viral RNA promoter stem loop A (SLA)-binding site on Zika virus polymerase NS5. Scientific reports 20 32764551
2020 The FAST Pump, a low-cost, easy to fabricate, SLA-3D-printed peristaltic pump for multi-channel systems in any lab. HardwareX 20 35498250
2015 Early bone response to machined, sandblasting acid etching (SLA) and novel surface-functionalization (SLAffinity) titanium implants: characterization, biomechanical analysis and histological evaluation in pigs. Journal of biomedical materials research. Part A 19 26418567
2008 Sequence-based characterization of the eight SLA loci in Korean native pigs. International journal of immunogenetics 19 18549392
2002 Expansion of the Knops blood group system and subdivision of Sl(a). Transfusion 19 11896343
2020 Porcine Reproductive and Respiratory Syndrome Virus Promotes SLA-DR-Mediated Antigen Presentation of Nonstructural Proteins To Evoke a Nonneutralizing Antibody Response In Vivo. Journal of virology 18 32796065
2017 Analysis of Novel Interactions between Components of the Selenocysteine Biosynthesis Pathway, SEPHS1, SEPHS2, SEPSECS, and SECp43. Biochemistry 18 28414460
2016 SLA-PGN-primed dendritic cell-based vaccination induces Th17-mediated protective immunity against experimental visceral leishmaniasis: a crucial role of PKCβ. Pathogens and disease 18 27150838
1988 Influence of SLA haplotype on preimplantation embryonic cell number in miniature pigs. Journal of reproduction and fertility 18 3141620
2021 Evaluation of Cellular Immunity with ASFV Infection by Swine Leukocyte Antigen (SLA)-Peptide Tetramers. Viruses 17 34835070
2017 Cytokines and chemokines measured in dried SLA-stimulated whole blood spots for asymptomatic Leishmania infantum and Leishmania donovani infection. Scientific reports 17 29222521
2012 Identification of peptides from foot-and-mouth disease virus structural proteins bound by class I swine leukocyte antigen (SLA) alleles, SLA-1*0401 and SLA-2*0401. Animal genetics 17 22984928
2009 Functional analyses of Src-like adaptor (SLA), a glucocorticoid-regulated gene in acute lymphoblastic leukemia. Leukemia research 17 19631983
2002 Development of a rapid in vitro protein refolding assay which discriminates between peptide-bound and peptide-free forms of recombinant porcine major histocompatibility class I complex (SLA-I). Veterinary immunology and immunopathology 17 11943330
1989 Genetic and other effects on bacterial phagocytosis and killing by cultured peripheral blood monocytes of SLA-defined miniature pigs. Animal genetics 17 2515776
1982 Transplantation in miniature swine. XII. N-terminal sequences of class I histocompatibility antigens (SLA) and beta 2-microglobulin. Journal of immunology (Baltimore, Md. : 1950) 17 6177778
2020 SLA-1 Genetic Diversity in Pigs: Extensive Analysis of Copy Number Variation, Heterozygosity, Expression, and Breed Specificity. Scientific reports 16 31959823
2020 Illumination of PRRSV Cytotoxic T Lymphocyte Epitopes by the Three-Dimensional Structure and Peptidome of Swine Lymphocyte Antigen Class I (SLA-I). Frontiers in immunology 16 31969884
2016 Lymphoproliferative response after stimulation with soluble leishmania antigen (SLA) as a predictor of visceral leishmaniasis (VL) relapse in HIV+ patients. Acta tropica 16 27693332
2010 Simple and comprehensive SLA-DQB1 genotyping using genomic PCR and direct sequencing. Tissue antigens 16 20604896
2005 Development of dense microsatellite markers in the entire SLA region and evaluation of their polymorphisms in porcine breeds. Immunogenetics 16 16189668
2004 Sequence and mRNA expression of nonclassical SLA class I genes SLA-7 and SLA-8. Immunogenetics 16 15118849
1993 Common swine leucocyte antigen (SLA) haplotypes in NIH and Sinclair miniature swine have similar effects on the expression of an inherited melanoma. Animal genetics 16 8363104
2023 Osteogenic and anti-inflammatory effects of SLA titanium substrates doped with chitosan-stabilized selenium nanoparticles via a covalent coupling strategy. Colloids and surfaces. B, Biointerfaces 15 36868181
2023 The non-classical major histocompatibility complex II protein SLA-DM is crucial for African swine fever virus replication. Scientific reports 15 37604847
2018 A SEPSECS mutation in a 23-year-old woman with microcephaly and progressive cerebellar ataxia. Journal of inherited metabolic disease 15 29464431
2017 Specificity Characterization of SLA Class I Molecules Binding to Swine-Origin Viral Cytotoxic T Lymphocyte Epitope Peptides in Vitro. Frontiers in microbiology 15 29326671
2010 Human SepSecS or SLA/LP: selenocysteine formation and autoimmune hepatitis. Biological chemistry 15 20623998
2015 Improved osseointegration of long-term stored SLA implant by hydrothermal sterilization. Journal of the mechanical behavior of biomedical materials 14 26382972
2011 Evaluation of chemically modified SLA implants (modSLA) biofunctionalized with integrin (RGD)- and heparin (KRSR)-binding peptides. Journal of biomedical materials research. Part A 14 22213622
1997 Antibodies to P450IID6, SLA, PDH-E2 and BCKD-E2 in Japanese patients with chronic hepatitis. Journal of gastroenterology and hepatology 14 9504898
2013 Structural mimicry between SLA/LP and Rickettsia surface antigens as a driver of autoimmune hepatitis: insights from an in silico study. Theoretical biology & medical modelling 13 23575112
2021 Critical Role of Etching Parameters in the Evolution of Nano Micro SLA Surface on the Ti6Al4V Alloy Dental Implants. Materials (Basel, Switzerland) 12 34771869
2015 Swine leukocyte antigen class II genes (SLA-DRA, SLA-DRB1, SLA-DQA, SLA-DQB1) polymorphism and genotyping in Guizhou minipigs. Genetics and molecular research : GMR 12 26634489
2012 Prediction of altered 3'- UTR miRNA-binding sites from RNA-Seq data: the swine leukocyte antigen complex (SLA) as a model region. PloS one 12 23139801
2021 Porcine Epidemic Diarrhea Virus Envelope Protein Blocks SLA-DR Expression in Barrow-Derived Dendritic Cells by Inhibiting Promoters Activation. Frontiers in immunology 11 34858400
2020 Human Salivary Histatin-1 Promotes Osteogenic Cell Spreading on Both Bio-Inert Substrates and Titanium SLA Surfaces. Frontiers in bioengineering and biotechnology 11 33195147
2016 Enhancement of osseointegration by direct coating of rhBMP-2 on target-ion induced plasma sputtering treated SLA surface for dental application. Journal of biomaterials applications 11 27881639
2005 Novel SLA-DQ alleles and their recombinant molecules in xenogeneic stimulation of human T cells. Transplant immunology 11 15935298
1999 DNA profiling reveals remarkably low genetic variability in a herd of SLA homozygous pigs. Research in veterinary science 11 10502496
2024 A neural pathway for social modulation of spontaneous locomotor activity (SoMo-SLA) in Drosophila. Proceedings of the National Academy of Sciences of the United States of America 10 38394240
2020 Association of Porcine Swine Leukocyte Antigen (SLA) Haplotypes with B- and T-Cell Immune Response to Foot-and-Mouth Disease Virus (FMDV) Peptides. Vaccines 10 32911818
2000 Influence of glutaraldehyde fixation on the detection of SLA-I and II antigens and calcification tendency in porcine cardiac tissue. Scandinavian cardiovascular journal : SCJ 10 11214013
1993 Characterization of a polymorphic heat shock protein 70 gene in swine outside the SLA major histocompatibility complex. Biochimica et biophysica acta 10 7687468
1986 Relationship between anemia and cholesterol metabolism in 'sex-linked anemic' (gene symbol, sla) mouse. Biochimica et biophysica acta 10 2874831
2022 Antimicrobial peptide GL13K immobilized onto SLA-treated titanium by silanization: antibacterial effect against methicillin-resistant Staphylococcus aureus (MRSA). RSC advances 9 35424597
2020 Biocorrosion of pure and SLA titanium surfaces in the presence of Porphyromonas gingivalis and its effects on osteoblast behavior. RSC advances 9 35497867
2018 The role of MPL and imiquimod adjuvants in enhancement of immune response and protection in BALB/c mice immunized with soluble Leishmania antigen (SLA) encapsulated in nanoliposome. Artificial cells, nanomedicine, and biotechnology 9 29607698
2018 Impact of Nano-Crystalline Diamond Enhanced Hydrophilicity on Cell Proliferation on Machined and SLA Titanium Surfaces: An In-Vivo Study in Rodents. Nanomaterials (Basel, Switzerland) 9 30011802
2004 Novel SLA-DR alleles of three Chinese pig strains and the related function in human T cell response. Cellular & molecular immunology 9 16219170
2003 Novel SLA class I alleles of Chinese pig strains and their significance in xenotransplantation. Cell research 9 12974618