| 2015 |
RNF152 is a lysosome-anchored E3 ubiquitin ligase that targets RagA for K63-linked polyubiquitination in an amino-acid-sensitive manner, thereby recruiting the GATOR1 GAP complex to RagA and negatively regulating mTORC1 activation at the lysosome. RNF152 knockout results in hyperactivation of mTORC1 and protects cells from amino-acid-starvation-induced autophagy. |
Co-immunoprecipitation, ubiquitination assays, RagA ubiquitination-site mutagenesis, RNF152 knockout cells with mTORC1 activity and autophagy readouts |
Molecular cell |
High |
25936802
|
| 2010 |
RNF152 is a canonical RING finger E3 ubiquitin ligase that localizes to lysosomes (co-localized with LAMP3), undergoes K48-linked polyubiquitination in a manner dependent on its RING finger and transmembrane domains, and promotes apoptosis when overexpressed in HeLa cells. |
Co-localization with lysosomal marker LAMP3, in vivo ubiquitination assays with domain deletion/mutation analysis, flow cytometry for apoptosis upon overexpression |
Protein & cell |
Medium |
21203937
|
| 2020 |
RNF152 positively regulates TLR/IL-1R signaling by directly interacting with the adaptor protein MyD88 and enhancing MyD88 oligomerization, which is required for recruitment of downstream signaling components; this effect is independent of RNF152's E3 ligase activity. RNF152-deficient mice produce less inflammatory cytokines in response to LPS and are more resistant to LPS-induced lethal endotoxemia. |
Co-immunoprecipitation of RNF152 with MyD88, MyD88 oligomerization assays, RNF152 overexpression/knockdown with NF-κB reporter assays, RNF152-deficient mouse model with cytokine measurements and endotoxemia model |
EMBO reports |
High |
31930677
|
| 2022 |
RNF152 acts as a negative regulator of Wnt/β-catenin signaling in Xenopus embryos by inhibiting Dishevelled polymerization in an E3 ligase-independent manner (requires the transmembrane domain but not ligase activity). Knockdown of RNF152 enhances Wnt-dependent transcriptional responses and neural crest formation, while overexpression inhibits β-catenin stabilization and target gene expression. |
Overexpression and morpholino knockdown in Xenopus embryos, Wnt-responsive reporter assays, ligase-dead and transmembrane-domain truncation mutants, Dishevelled polymerization assays |
BMB reports |
Medium |
35410636
|
| 2023 |
RNF152 binds IRAK1 and promotes its K48-linked ubiquitination and proteasomal degradation, thereby reducing IRAK1-dependent transcription of AKR1B10 and suppressing fatty acid oxidation and metastasis in lung adenocarcinoma cells. |
Co-immunoprecipitation of RNF152 with IRAK1, ubiquitination assays, protein stability assays, RNF152 overexpression/knockdown with AKR1B10 expression and fatty acid oxidation readouts, IRAK1 rescue experiments, mouse xenograft models |
The American journal of pathology |
Medium |
37717980
|
| 2024 |
Fasting-induced RNF152 ubiquitinates the Ragulator subunit p18, leading to its proteasomal degradation; loss of RNF152 increases lysosomal localization of p18 and mTORC1 activity, promoting glycolysis and gemcitabine resistance in gallbladder cancer cells. |
Co-immunoprecipitation, ubiquitination assays, proteasome inhibitor experiments, RNF152 overexpression/silencing with mTORC1 activity, glycolysis, and drug sensitivity readouts, lentiviral transfections |
iScience |
Medium |
38706841
|
| 2025 |
RNF152 directly interacts with HSP27 and ubiquitinates it at Lys114, targeting HSP27 for proteasomal degradation; RNF152-mediated HSP27 degradation activates the PI3K/AKT pathway, driving fibroblast-like synoviocyte proliferation and pro-inflammatory cytokine release in temporomandibular joint osteoarthritis. |
Immunoprecipitation-mass spectrometry, co-immunoprecipitation, co-localization imaging, protein docking, site-specific ubiquitination (Lys114) assays, in vivo AAV-siRNF152 rat TMJOA model |
Arthritis research & therapy |
Medium |
41413572
|
| 2017 |
Zebrafish rnf152 (ortholog of human RNF152) is required for Delta-Notch signaling and NeuroD expression during brain development; knockdown abolishes NeuroD expression in retinal layers and reduces deltaD, notch1a, notch3, and Notch target genes her4 and ascl1a in midbrain, hindbrain, and rhombomeres. |
Morpholino knockdown in zebrafish embryos, whole-mount in situ hybridization for neuroD, deltaD, notch1a, notch3, her4, ascl1a |
Molecules and cells |
Medium |
29276941
|
| 2024 |
RNF152, as a short-lived lysosomal membrane protein, can be co-opted by bifunctional LYMTAC small molecules to tether KRAS G12D to the lysosomal membrane, inducing KRAS relocalization, inhibition of downstream phospho-ERK signaling, and lysosomal degradation of KRAS G12D. |
Heterobifunctional small molecule (LYMTAC) treatment, KRAS relocalization imaging, phospho-ERK signaling assays, lysosomal degradation assays in cell lines |
bioRxivpreprint |
Low |
bio_10.1101_2024.09.08.611923
|
| 2021 |
Overexpression of RNF152 in RKO colon cancer cells increases apoptosis and sensitizes cells to NO donor-induced apoptosis, accompanied by decreased expression of anti-apoptotic proteins Bcl-XL and Bcl-2. |
RNF152 overexpression in RKO cells, flow cytometry for apoptosis, western blot for Bcl-2 and Bcl-XL |
Zhonghua zhong liu za zhi |
Low |
34034471
|