Affinage

RCVRN

Recoverin · UniProt P35243

Length
200 aa
Mass
23.1 kDa
Annotated
2026-06-10
28 papers in source corpus 2 papers cited in narrative 2 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 3/4 claims corpus-supported (75%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

RCVRN encodes recoverin, a photoreceptor-specific protein that is identical to the cancer-associated retinopathy (CAR) antigen, establishing a single gene product underlying both the retinal protein and the autoimmune retinopathy target (PMID:8422501). The protein contains two canonical Ca2+-binding EF-hand motifs as inferred from sequence analysis of the cloned gene (PMID:12447165). Beyond this sequence-level characterization and chromosomal mapping near Trp53 (PMID:8422501), no direct mechanistic detail on its enzymatic activity, calcium-dependent conformational behavior, or binding partners has been characterized in the available corpus. Candidate-gene analysis of the canine ortholog found that coding substitutions did not segregate with progressive retinal atrophy, providing negative genetic evidence against this gene as the cause of that disorder (PMID:12447165).

Mechanistic history

Synthesis pass · year-by-year structured walk · 2 steps
  1. 1993 Medium

    Resolved whether the photoreceptor 23 kDa protein, recoverin, and the CAR antigen represent the same molecule, unifying a retinal protein with an autoimmune retinopathy target.

    Evidence Deduced amino acid sequence comparison of the mouse Rcvrn gene plus restriction fragment length variant segregation mapping in recombinant inbred strains

    PMID:8422501

    Open questions at the time
    • No functional or biochemical activity assigned to the protein
    • Calcium-binding behavior not experimentally tested
    • Mechanism linking the protein to the CAR autoimmune phenotype not addressed
  2. 2002 Low

    Characterized the canine ortholog's structural features and tested it as a candidate disease gene, defining its EF-hand architecture and excluding it as a cause of progressive retinal atrophy.

    Evidence Genomic library cloning, PCR-SSCP, DNA sequencing, and breed-wide segregation analysis across 22 dog breeds

    PMID:12447165

    Open questions at the time
    • EF-hand calcium-binding activity inferred from sequence only, not functionally validated
    • Negative genetic result from single-lab segregation analysis
    • No mechanistic role established

Open questions

Synthesis pass · forward-looking unresolved questions
  • The calcium-dependent conformational mechanism, enzymatic or regulatory activity, binding partners, and downstream signaling role of recoverin remain uncharacterized in the available corpus.
  • No direct measurement of calcium binding by the EF-hand motifs
  • No identified physical partners or substrates
  • No structural or functional characterization of the photoreceptor role

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
No controlled-vocabulary terms were assigned to this entry.

Evidence

Reading pass · 2 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1993 The mouse 23 kDa photoreceptor-specific protein (Rcvrn) was shown by deduced amino acid sequence comparison to be identical to the recoverin protein and the CAR (cancer-associated retinopathy) antigen, establishing these as the same gene product. Segregation analysis of restriction fragment length variants in recombinant inbred mouse strains mapped Rcvrn to Chromosome 11, between Sparc and Zfp-3, in close linkage with the tumor suppressor gene Trp53. DNA sequence analysis of mouse Rcvrn gene; restriction fragment length variant segregation analysis in recombinant inbred strains Mammalian genome : official journal of the International Mammalian Genome Society Medium 8422501
2002 The canine RCV1 protein (202 amino acids) contains two canonical Ca2+-binding EF-hand motifs, as determined by sequence analysis of the cloned gene. Amino acid substitutions N3K and P202H identified in the canine ORF did not segregate with progressive retinal atrophy (PRA) in any of 22 dog breeds examined, providing negative evidence against RCV1 as the cause of PRA. Genomic library cloning, PCR-SSCP, DNA sequencing, breed-wide segregation analysis Molecular vision Low 12447165

Source papers

Stage 0 corpus · 28 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2016 3D culture of human pluripotent stem cells in RGD-alginate hydrogel improves retinal tissue development. Acta biomaterialia 120 27826002
2011 Differentiation of induced pluripotent stem cells of swine into rod photoreceptors and their integration into the retina. Stem cells (Dayton, Ohio) 96 21491544
1995 A gene for autosomal dominant progressive cone dystrophy (CORD5) maps to chromosome 17p12-p13. Genomics 71 8586428
2017 miR-365 promotes diabetic retinopathy through inhibiting Timp3 and increasing oxidative stress. Experimental eye research 47 29196060
2015 Characterization of Three-Dimensional Retinal Tissue Derived from Human Embryonic Stem Cells in Adherent Monolayer Cultures. Stem cells and development 47 26283078
1996 Localisation of a gene for central areolar choroidal dystrophy to chromosome 17p. Human molecular genetics 45 8733141
2018 Intravitreal Injection of Hydrogen Peroxide Induces Acute Retinal Degeneration, Apoptosis, and Oxidative Stress in Mice. Oxidative medicine and cellular longevity 30 30533172
1993 Chromosomal assignment of the recoverin gene and cancer-associated retinopathy. Mammalian genome : official journal of the International Mammalian Genome Society 22 8422501
1995 Evidence against involvement of recoverin in autosomal recessive retinitis pigmentosa in 42 Spanish families. Human genetics 20 7607661
2024 Retinal organoids with X-linked retinoschisis RS1 (E72K) mutation exhibit a photoreceptor developmental delay and are rescued by gene augmentation therapy. Stem cell research & therapy 18 38816767
2014 Swine cone and rod precursors arise sequentially and display sequential and transient integration and differentiation potential following transplantation. Investigative ophthalmology & visual science 18 24327609
2022 Timed Notch Inhibition Drives Photoreceptor Fate Specification in Human Retinal Organoids. Investigative ophthalmology & visual science 17 36129723
2020 Generation of a Retina Reporter hiPSC Line to Label Progenitor, Ganglion, and Photoreceptor Cell Types. Translational vision science & technology 16 32714647
2022 Generation of an RCVRN-eGFP Reporter hiPSC Line by CRISPR/Cas9 to Monitor Photoreceptor Cell Development and Facilitate the Cell Enrichment for Transplantation. Frontiers in cell and developmental biology 14 35573687
2021 Knock-Out of Tenascin-C Ameliorates Ischemia-Induced Rod-Photoreceptor Degeneration and Retinal Dysfunction. Frontiers in neuroscience 14 34093110
2016 Molecular studies of phenotype variation in canine RPGR-XLPRA1. Molecular vision 12 27122963
2018 A Wor1-Like Transcription Factor Is Essential for Virulence of Cryptococcus neoformans. Frontiers in cellular and infection microbiology 10 30483479
2022 RNA-Seq analysis reveals sex-dependent transcriptomic profiles of human subacromial bursa stratified by tear etiology. Journal of orthopaedic research : official publication of the Orthopaedic Research Society 8 35266580
2022 Legumain affects the PI3K/AKT tumor progression pathway in retinoblastoma. Experimental eye research 8 36041510
2002 The canine Recoverin (RCV1) gene: a candidate gene for generalized progressive retinal atrophy. Molecular vision 8 12447165
2000 Measurement of plasma volume using 99Tc(m)-labelled DMP-HSA. Nuclear medicine communications 8 10894563
2021 Downregulation of CRX, a Group 3-specific oncogenic transcription factor, inhibits TGF-β/activin signaling in medulloblastoma cells. Biochemical and biophysical research communications 6 34192607
2017 Proliferative Cells Isolated from the Adult Human Peripheral Retina only Transiently Upregulate Key Retinal Markers upon Induced Differentiation. Current eye research 6 29161152
2024 NR2E3 inhibits the inflammation and apoptosis in diabetic retinopathy by regulating the AHR/IL-17A signaling pathway. Naunyn-Schmiedeberg's archives of pharmacology 4 38884674
2013 Irx7, a Smarca4-regulated gene for retinal differentiation, regulates other genes controlled by Smarca4 in zebrafish retinas. Gene expression patterns : GEP 4 23557786
2023 The probiotic-induced disregulation of immune-related genes in colon cells and relation with colorectal cancer. Cellular and molecular biology (Noisy-le-Grand, France) 1 37329550
2026 Short-term exposure of sleep deprivation male model rats to dutasteride promotes the discovery of gene profiles related to fertility impairments. BMC medical genomics 0 41742262
2025 A comparative transcriptomic analysis provides new insights into the ecological adaptations in Ichthyophis bannanicus. BMC genomics 0 41327005

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