Affinage

PRUNE1

Exopolyphosphatase PRUNE1 · UniProt Q86TP1

Length
453 aa
Mass
50.2 kDa
Annotated
2026-06-10
100 papers in source corpus 18 papers cited in narrative 18 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/7 claims corpus-supported (86%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PRUNE1 (h-prune) is a DHH-superfamily phosphoesterase that drives cell motility, cytoskeletal remodeling, and metastasis through coupled enzymatic and scaffolding functions (PMID:14998490, PMID:16428445). Its N-terminal catalytic domain is a metal-dependent enzyme with cyclic-nucleotide phosphodiesterase activity and short-chain exopolyphosphatase activity, defined by conserved active-site residues; PDE activity lowers cellular cAMP and is required for its biological effects (PMID:14998490, PMID:18700747, PMID:15254413, PMID:22967741). A structurally independent C-terminal cortexillin-homology domain is largely unfolded and mediates homodimerization and protein-protein interactions (PMID:17655525, PMID:23939913). Through this domain PRUNE1 binds the metastasis suppressor NM23-H1/NME1 in a manner requiring CKIδ/ε phosphorylation of NM23-H1 across its S120–S125 region, an interaction that stimulates PRUNE1 enzymatic activity and promotes tumor cell motility and metastasis (PMID:10602478, PMID:17906697, PMID:23448979). PRUNE1 also binds GSK-3β in a kinase-activity-dependent manner and localizes to focal adhesions, where it promotes paxillin disassembly, FAK phosphorylation, and Rac activation to enable migration, and engages canonical WNT/β-catenin signaling (PMID:16428445, PMID:25026278). In metastatic medulloblastoma it activates a TGF-β/OTX2/SNAIL/PTEN axis downstream of NME1 binding, and in breast cancer it reprograms tumor-associated macrophages toward an M2 phenotype via TGF-β and IL-17F (PMID:29490009, PMID:33426510). Loss-of-function missense variants in the conserved N-terminal DHH motif that destabilize the protein or abolish exopolyphosphatase activity cause the autosomal recessive neurodevelopmental disorder NMIHBA, partly through impaired microtubule polymerization and cell migration, and complete Prune1 ablation in mice is midgestationally lethal (PMID:28334956, PMID:33105479).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1999 High

    Establishing that PRUNE1 physically partners with the metastasis suppressor NM23-H1 placed it directly within metastasis-suppression biology and defined its first molecular interaction.

    Evidence Yeast two-hybrid interaction mating, reciprocal co-IP, and cytoplasmic co-localization, with loss of binding to the NM23-H1-S120G mutant

    PMID:10602478

    Open questions at the time
    • Functional consequence of the interaction not yet established
    • Interaction domain on PRUNE1 not mapped
    • Biochemical activity of PRUNE1 unknown at this stage
  2. 2004 High

    Identifying cAMP-PDE activity and linking it to NM23-H1 binding and cell motility converted PRUNE1 from a binding partner into an enzymatically active metastasis driver.

    Evidence In vitro cAMP-PDE assay with dipyridamole inhibition, Co-IP, and motility assays in breast cancer lines; overexpression lowers cAMP and raises motility

    PMID:14998490 PMID:15254413

    Open questions at the time
    • Catalytic residues not yet defined
    • Whether PDE activity alone suffices for motility unresolved
    • Substrate range beyond cAMP unexplored
  3. 2006 High

    Discovery that PRUNE1 binds GSK-3β and localizes to focal adhesions defined a cytoskeletal mechanism linking it to migration machinery.

    Evidence Reciprocal Co-IP, siRNA knockdown, focal adhesion immunofluorescence, paxillin disassembly, FAK phosphorylation, and Rac activation assays; gelsolin identified by pull-down/MS

    PMID:16428445 PMID:17103319

    Open questions at the time
    • How GSK-3β binding couples to PDE activity unclear
    • Gelsolin interaction not mechanistically followed up
    • Direct vs indirect effects on FAK/Rac not dissected
  4. 2007 Medium

    Mapping the two-domain architecture and the CKI-dependent NM23-H1 interaction defined how enzymatic and scaffolding functions are physically partitioned and regulated.

    Evidence Limited proteolysis and deletion mapping defined an N-terminal PDE domain and C-terminal cortexillin dimerization/binding domain; in vitro CKIδ/ε kinase assay, IC261 inhibition, and competitive peptide blocked complex formation and motility

    PMID:17655525 PMID:17906697

    Open questions at the time
    • No high-resolution structure of either domain
    • Stoichiometry of dimer/NM23-H1 complex unresolved
    • In vivo relevance of CKI phosphorylation not tested
  5. 2008 High

    Reconstitution of short-chain exopolyphosphatase activity with active-site mutagenesis revealed a second catalytic activity beyond cAMP hydrolysis.

    Evidence In vitro assays with recombinant protein showing metal-dependent hydrolysis of tri/tetrapolyphosphates, substrate profiling, and severe activity loss upon mutation of seven yeast-homologous active-site residues

    PMID:18700747

    Open questions at the time
    • Physiological polyP substrate in cells not identified
    • Relationship between PDE and exopolyphosphatase activities unclear
    • Cellular consequences of polyP hydrolysis untested at this stage
  6. 2012 Medium

    Demonstrating that PDE-dead mutants lose gliogenic activity established PDE catalysis as functionally required in a developmental in vivo context.

    Evidence Xenopus overexpression with active-site aspartate substitutions and Müller glial cell fate quantification

    PMID:22967741

    Open questions at the time
    • cAMP target downstream of PDE in this context not defined
    • Relevance to mammalian neurodevelopment not established
    • Whether scaffolding contributes independently untested
  7. 2013 Medium

    NMR mapping of the unfolded C-terminal domain identified the residues engaging NM23-H1, GSK-3β, and gelsolin and validated peptide-based inhibition as anti-metastatic.

    Evidence In-lysate NMR conformational analysis plus competitive permeable peptide reducing complex formation, cell motility, and tumor growth/metastasis in neuroblastoma xenografts

    PMID:23448979 PMID:23939913

    Open questions at the time
    • No folded structure of the interaction interfaces
    • Selectivity of the peptide across the three partners unclear
    • Whether all three interactions occur simultaneously unknown
  8. 2014 Medium

    Linking the GSK-3β interaction to canonical WNT/β-catenin activation and Wnt3a secretion extended PRUNE1 into a pro-metastatic signaling pathway.

    Evidence Co-IP, WNT/β-catenin reporter assay, Wnt3a ELISA, and in vivo lung metastasis model with siRNA silencing

    PMID:25026278

    Open questions at the time
    • Mechanism by which PRUNE1 modulates GSK-3β toward WNT unclear
    • Direct vs paracrine contributions not separated
    • Requirement of enzymatic activity not tested
  9. 2015 Medium

    Mitochondrial-matrix localization of the Drosophila ortholog and its control of TFAM/mtDNA placed PRUNE1's PDE activity within mitochondrial cAMP signaling and bioenergetics.

    Evidence Subcellular fractionation, RNAi knockdown, and measurement of TFAM levels and mtDNA copy number in Drosophila

    PMID:25648146

    Open questions at the time
    • Mitochondrial localization not confirmed for human PRUNE1
    • Mechanism linking cAMP-PDE to TFAM stability indirect
    • Conservation of mtDNA function in mammals untested
  10. 2018 Medium

    Defining a PRUNE1/NME1–TGF-β–OTX2–SNAIL–PTEN axis in group 3 medulloblastoma and showing druggability connected its interactions to a specific oncogenic program.

    Evidence Co-IP, pathway activation assays, competitive permeable peptide, and the degradation-enhancing small molecule AA7.1 in orthotopic xenografts

    PMID:29490009

    Open questions at the time
    • Direct molecular steps between NME1 binding and TGF-β activation unresolved
    • Whether enzymatic activity is required for this axis unclear
    • Generality beyond medulloblastoma untested
  11. 2020 Medium

    Showing PRUNE1 drives M2 macrophage polarization extended its pro-metastatic role into the tumor microenvironment.

    Evidence PRUNE1-overexpression genetically engineered TNBC mouse model with macrophage polarization, TGF-β/IL-17F secretion, and extracellular vesicle analyses

    PMID:33426510

    Open questions at the time
    • Whether tumor-cell-intrinsic enzymatic activity drives the secretome unclear
    • Cargo of extracellular vesicles not defined
    • Causal role of IL-17F not isolated
  12. 2021 High

    Linking DHH-motif missense variants to loss of exopolyphosphatase activity and an embryonic-lethal knockout established that catalytic function is required for normal neurodevelopment and the disease NMIHBA.

    Evidence Biochemical stability and enzymatic assays of recombinant disease variants plus a Prune1 knockout mouse with midgestational lethality and vascular defects

    PMID:33105479

    Open questions at the time
    • Physiological polyP substrate in neural tissue unidentified
    • Mechanism linking enzymatic loss to cortical phenotype indirect
    • Conditional/tissue-specific requirements not dissected
  13. 2023 Medium

    Reassessing polyphosphate substrates and connecting PRUNE1 to ATP synthase activity reframed its effect on cellular polyP as indirect via mitochondrial bioenergetics.

    Evidence In vitro assays with defined polyP chain lengths (showing no hydrolysis of 13–160 Pi chains) and HEK293 knockdown reducing cellular polyP, ATP synthase activity, and ATP, with ATP5A compensation

    PMID:37762163

    Open questions at the time
    • Direct molecular link between PRUNE1 and ATP synthase not defined
    • Reconciliation with earlier short-chain exopolyphosphatase data incomplete
    • Whether mitochondrial localization mediates the effect in human cells unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • How PRUNE1's two catalytic activities, its scaffolding interactions, and its mitochondrial/bioenergetic effects are mechanistically integrated to produce both neurodevelopmental and metastatic phenotypes remains unresolved.
  • No high-resolution full-length structure
  • Endogenous physiological substrate(s) of the enzyme unidentified
  • Direct molecular bridge from enzymatic activity to cytoskeletal/microtubule defects unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016787 hydrolase activity 4 GO:0060089 molecular transducer activity 3 GO:0140096 catalytic activity, acting on a protein 3
Localization
GO:0005739 mitochondrion 1 GO:0005829 cytosol 1
Pathway
R-HSA-1266738 Developmental Biology 2 R-HSA-162582 Signal Transduction 2 R-HSA-1643685 Disease 2
Partners
CSNK1DGSK3BGSNNME1

Evidence

Reading pass · 18 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2004 Human prune (h-prune) possesses cyclic nucleotide phosphodiesterase (cAMP-PDE) activity that can be suppressed by dipyridamole, and physically interacts with nm23-H1 (a metastasis suppressor); the interaction with nm23-H1 enhances h-prune PDE activity and stimulates cellular motility and metastasis. In vitro enzymatic assay (cAMP-PDE activity), co-immunoprecipitation, dipyridamole inhibition, cellular motility assays in breast cancer cell lines Cancer cell High 14998490
1999 Human PRUNE protein interacts with nm23-H1 (human homologue of awd/NDP kinase) as shown by interaction-mating and in vitro co-immunoprecipitation; PRUNE is impaired in interaction with the nm23-H1-S120G gain-of-function mutant; both proteins partially co-localize in the cytoplasm. Yeast two-hybrid interaction mating, co-immunoprecipitation, subcellular co-localization Oncogene High 10602478
2008 h-prune efficiently hydrolyzes short-chain polyphosphates (tri- and tetrapolyphosphates, nucleoside 5'-tetraphosphates) as a short-chain exopolyphosphatase, requiring divalent metal cofactors (Mg2+, Co2+, Mn2+); long-chain polyphosphates are hydrolyzed more slowly; pyrophosphate and nucleoside triphosphates are not hydrolyzed. Mutation of seven active-site residues corresponding to yeast exopolyphosphatase severely reduced activity. The exopolyphosphatase activity is suppressed by nm23-H1, long-chain polyphosphates, and pyrophosphate. In vitro enzymatic assay with recombinant protein, active-site mutagenesis, substrate specificity profiling Biochemistry High 18700747
2006 h-prune was identified as a glycogen synthase kinase 3 (GSK-3) binding protein; the kinase activity of GSK-3 is required for this interaction. h-prune localizes to focal adhesions, and siRNA knockdown of GSK-3 or h-prune delays disassembly of paxillin, suppresses tyrosine phosphorylation of FAK, and suppresses Rac activation, impairing cell motility. Co-immunoprecipitation, siRNA knockdown, immunofluorescence localization to focal adhesions, paxillin disassembly assay, FAK phosphorylation assay, Rac activation assay Molecular and cellular biology High 16428445
2007 The region of nm23-H1 spanning S120–S125 mediates the nm23-H1/h-prune interaction; phosphorylation of nm23-H1 at this region by casein kinase I (CKI) delta/epsilon is required for complex formation. The CKI delta/epsilon-specific inhibitor IC261 impairs complex formation and inhibits cellular motility in a breast cancer model. A competitive permeable peptide spanning the CKI phosphorylation region similarly impairs motility. Mutational mapping of interaction site, in vitro kinase assay (CKI phosphorylation), pharmacological inhibition with IC261, competitive peptide inhibition, transwell cell migration assay Oncogene High 17906697
2007 h-prune contains two structurally independent domains: an N-terminal PDE catalytic domain (active even as monomer) and a C-terminal cortexillin homology domain that mediates h-prune homodimerization and interaction with NM23-H1. Domain boundaries were identified by sequence analysis and limited proteolysis of recombinant h-prune. Recombinant protein expression, limited proteolysis, sequence analysis, domain mapping by deletion constructs, dimerization and binding assays The Biochemical journal Medium 17655525
2013 NMR spectroscopy of the h-prune C-terminal domain in human cell lysates identified the amino acids of this largely unfolded domain involved in interactions with Nm23-H1, GSK-3β, and gelsolin; a competitive permeable peptide (CPP) derived from this region impairs Nm23-H1/h-prune complex formation, inhibits cell motility, and substantially reduces tumor growth and metastasis in neuroblastoma models. NMR spectroscopy in cell lysates, conformational analysis, competitive peptide inhibition, cell motility assay, in vivo orthotopic xenograft tumor model Scientific reports Medium 23448979
2013 NMR spectroscopy mapping of h-prune C-terminal domain confirmed it is largely unfolded and mediates protein-protein interactions with Nm23-H1, GSK-3β, and gelsolin. Fast NMR spectroscopy in cell lysates Chemistry (Weinheim an der Bergstrasse, Germany) Medium 23939913
2017 Disease-associated PRUNE1 variant alleles cause impaired microtubule polymerization, and reduced cell migration and proliferation. These functional defects establish a role for PRUNE1 in microtubule polymerization essential for cytoskeletal rearrangements during cell division and proliferation in cortical brain development. Functional assays of disease variant alleles: microtubule polymerization assay, cell migration assay, cell proliferation assay Brain : a journal of neurology Medium 28334956
2015 Drosophila Prune (ortholog of human PRUNE1) is a cyclic nucleotide phosphodiesterase that localizes to the mitochondrial matrix. Knockdown of prune in cultured cells reduces mitochondrial transcription factor A (TFAM) and mtDNA levels; Prune stabilizes TFAM and promotes mtDNA replication through downregulation of mitochondrial cAMP signaling. Subcellular fractionation and localization (mitochondrial matrix), RNAi knockdown in cultured cells, measurement of TFAM protein levels and mtDNA copy number EMBO reports Medium 25648146
2014 h-prune interacts with GSK-3β and through this complex activates canonical WNT/β-catenin signaling, also in a paracrine manner via Wnt3a secretion. h-prune silencing inhibits lung metastasis formation in vivo. Co-immunoprecipitation (h-prune/GSK-3β interaction), WNT/β-catenin reporter assay, Wnt3a ELISA, in vivo lung metastasis model (siRNA silencing) Oncotarget Medium 25026278
2021 NMIHBA-associated missense variants within the conserved N-terminal DHH motif of PRUNE1 result in destabilization of protein structure and/or loss of exopolyphosphatase activity, establishing that exopolyphosphatase activity is required for normal neurodevelopment. Complete genetic ablation of Prune1 in mice causes midgestational lethality with perturbations to embryonic growth and vascular development. Biochemical characterization of recombinant missense variants (protein stability and enzymatic activity assays), Prune1 knockout mouse model Human molecular genetics High 33105479
2018 PRUNE1 activates a signaling pathway in metastatic medulloblastoma group 3 involving binding to NME1, TGF-β activation, OTX2 upregulation, SNAIL (SNAI1) upregulation, and PTEN inhibition. A competitive permeable peptide impairing PRUNE1/NME1 complex formation, and a small molecule (AA7.1) that enhances PRUNE1 degradation, both impair tumor growth and metastatic dissemination in orthotopic xenograft models. Co-immunoprecipitation (PRUNE1/NME1), competitive permeable peptide, small molecule inhibitor (AA7.1), orthotopic xenograft mouse model, pathway activation assays (TGF-β, OTX2, SNAIL, PTEN) Brain : a journal of neurology Medium 29490009
2023 Recombinant h-prune does not hydrolyze short (13–33 Pi) or medium (45–160 Pi) chain polyphosphates. However, knockdown of h-prune in HEK293 cells significantly decreases cellular polyP levels and reduces ATP synthase activity and ATP levels, with compensatory upregulation of ATP5A expression; these effects on mitochondrial bioenergetics are not due to loss of mitochondrial membrane potential or mitochondrial biomass. In vitro enzymatic assay with recombinant h-prune and defined polyphosphate chain lengths, siRNA knockdown in HEK293 cells, polyP quantification, ATP measurement, mitochondrial membrane potential assay, Western blot for ATP5A International journal of molecular sciences Medium 37762163
2006 Protein-protein pull-down analyses coupled with mass spectrometry identified gelsolin (an ATP-severing protein acting in focal adhesions) as a new h-prune binding partner in a breast cancer cellular model, in addition to the known GSK-3β interaction. Protein-protein pull-down assay coupled to mass spectrometry Journal of bioenergetics and biomembranes Low 17103319
2012 Xenopus prune overexpression in retinal precursor cells increases the ratio of Müller glial cells; a mutant form of prune with four aspartate (D) residue substitutions that abolish phosphodiesterase activity does not exhibit gliogenic activity, establishing that PDE activity is required for prune-mediated Müller gliogenesis. Xenopus overexpression, active-site mutagenesis (D residue substitutions), cell fate quantification (Müller glial cells) Gene Medium 22967741
2020 PRUNE1 overexpression promotes lung metastasis and M2-polarization of tumor-associated macrophages (TAMs) within the tumor microenvironment, mediated through TGF-β enhancement and IL-17F secretion, as shown in a genetically engineered mouse model of metastatic triple-negative breast cancer. Genetically engineered mouse model (PRUNE1 overexpression), macrophage polarization assays, TGF-β and IL-17F secretion measurements, extracellular vesicle analysis iScience Medium 33426510
2004 Overexpression of h-prune in the MDA-MB-435 breast carcinoma cell line causes a substantial decrease in cAMP levels and an increase in cellular motility, effects correlated with h-prune PDE activity and the h-prune/nm23-H1 protein interaction. Stable overexpression in breast cancer cell line, cAMP measurement, cell motility assay Cell cycle (Georgetown, Tex.) Medium 15254413

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1988 Analysis of the lethal interaction between the prune and Killer of prune mutations of Drosophila. Genes & development 142 2849580
2004 Prune cAMP phosphodiesterase binds nm23-H1 and promotes cancer metastasis. Cancer cell 121 14998490
1992 A Pro/Ser substitution in nucleoside diphosphate kinase of Drosophila melanogaster (mutation killer of prune) affects stability but not catalytic efficiency of the enzyme. The Journal of biological chemistry 112 1320004
2008 Human metastasis regulator protein H-prune is a short-chain exopolyphosphatase. Biochemistry 110 18700747
2006 Glycogen synthase kinase 3 and h-prune regulate cell migration by modulating focal adhesions. Molecular and cellular biology 106 16428445
1991 A product of the prune locus of Drosophila is similar to mammalian GTPase-activating protein. Nature 75 1654526
1999 Evidence for interaction between human PRUNE and nm23-H1 NDPKinase. Oncogene 68 10602478
2011 Muscarinic Acetylcholine Receptor M3 Mutation Causes Urinary Bladder Disease and a Prune-Belly-like Syndrome. American journal of human genetics 64 22077972
2017 PRUNE is crucial for normal brain development and mutated in microcephaly with neurodevelopmental impairment. Brain : a journal of neurology 56 28334956
2012 R179H mutation in ACTA2 expanding the phenotype to include prune-belly sequence and skin manifestations. American journal of medical genetics. Part A 51 22302747
2016 A plasma membrane microdomain compartmentalizes ephrin-generated cAMP signals to prune developing retinal axon arbors. Nature communications 50 27694812
2001 Amplification and overexpression of PRUNE in human sarcomas and breast carcinomas-a possible mechanism for altering the nm23-H1 activity. Oncogene 46 11687967
2007 Phosphorylation of nm23-H1 by CKI induces its complex formation with h-prune and promotes cell motility. Oncogene 41 17906697
2015 The cAMP phosphodiesterase Prune localizes to the mitochondrial matrix and promotes mtDNA replication by stabilizing TFAM. EMBO reports 39 25648146
2014 H-Prune through GSK-3β interaction sustains canonical WNT/β-catenin signaling enhancing cancer progression in NSCLC. Oncotarget 39 25026278
1995 Point mutations in awdKpn which revert the prune/Killer of prune lethal interaction affect conserved residues that are involved in nucleoside diphosphate kinase substrate binding and catalysis. The Journal of biological chemistry 39 7559441
2014 Supertrees Based on the Subtree Prune-and-Regraft Distance. Systematic biology 38 24695589
2011 Genetic basis of prune belly syndrome: screening for HNF1β gene. The Journal of urology 38 22114815
1983 Chromosome Abnormalities in infants with prune belly anomaly: association with trisomy 18. American journal of medical genetics 37 6859114
2010 Deletion of hepatocyte nuclear factor-1-beta in an infant with prune belly syndrome. American journal of perinatology 36 20175044
2005 Overexpression of h-prune in breast cancer is correlated with advanced disease status. Clinical cancer research : an official journal of the American Association for Cancer Research 36 15671547
1994 The complete nucleotide sequence of prune dwarf ilarvirus RNA 3: implications for coat protein activation of genome replication in ilarviruses. Virology 32 8178476
1978 Prostatic maldevelopment in the prune belly syndrome: a defect in prostatic stromal-epithelial interaction. The Journal of urology 32 28424
2013 Neuroblastoma tumorigenesis is regulated through the Nm23-H1/h-Prune C-terminal interaction. Scientific reports 31 23448979
2006 PRUNE and NM23-M1 expression in embryonic and adult mouse brain. Journal of bioenergetics and biomembranes 30 17033939
2009 The Nm23-H1-h-Prune complex in cellular physiology: a 'tip of the iceberg' protein network perspective. Molecular and cellular biochemistry 29 19390954
2018 Metastatic group 3 medulloblastoma is driven by PRUNE1 targeting NME1-TGF-β-OTX2-SNAIL via PTEN inhibition. Brain : a journal of neurology 28 29490009
2006 H-prune-nm23-H1 protein complex and correlation to pathways in cancer metastasis. Journal of bioenergetics and biomembranes 28 17103319
1977 The prune belly anomaly: heterogeneity and superficial X-linkage mimicry. Journal of medical genetics 26 144797
1996 Germline transformation using a prune cDNA rescues prune/killer of prune lethality and the prune eye color phenotype in Drosophila. Genetics 25 8978047
2021 Antioxidant and Anti-Inflammaging Ability of Prune (Prunus Spinosa L.) Extract Result in Improved Wound Healing Efficacy. Antioxidants (Basel, Switzerland) 24 33801467
2020 Prune-1 drives polarization of tumor-associated macrophages (TAMs) within the lung metastatic niche in triple-negative breast cancer. iScience 24 33426510
2024 PIEZO1 loss-of-function compound heterozygous mutations in the rare congenital human disorder Prune Belly Syndrome. Nature communications 23 38184690
1992 The lethal prune/Killer-of-prune interaction of Drosophila causes a syndrome resembling human neurofibromatosis (NF1). European journal of cell biology 22 1425777
1988 Testicular histology in fetuses with the prune belly syndrome and posterior urethral valves. The Journal of urology 22 3339735
2020 Prune belly syndrome in surviving males can be caused by Hemizygous missense mutations in the X-linked Filamin A gene. BMC medical genetics 21 32085749
1994 Polymorphism and divergence at the prune locus in Drosophila melanogaster and D. simulans. Molecular biology and evolution 20 8078405
1991 Isolation and characterization of the prune locus of Drosophila melanogaster. Genetics 20 1649071
2017 Homozygous mutation in PRUNE1 in an Oji-Cree male with a complex neurological phenotype. American journal of medical genetics. Part A 19 28211990
2003 Comparison of ELISA and RT-PCR for the detection of Prunus necrotic ring spot virus and prune dwarf virus in almond (Prunus dulcis). Journal of virological methods 19 14599680
1988 Interstitial deletion of chromosome 1 [del(1)(q25q32)] in an infant with prune belly sequence. Prenatal diagnosis 19 3375199
2023 Associations Between Inflammatory Mediators and Bone Outcomes in Postmenopausal Women: A Cross-Sectional Analysis of Baseline Data from the Prune Study. Journal of inflammation research 18 36814438
2009 DNA hypomethylation, transient neonatal diabetes, and prune belly sequence in one of two identical twins. European journal of pediatrics 18 19521719
2005 Gene locus ambiguity in posterior urethral valves/prune-belly syndrome. Pediatric nephrology (Berlin, Germany) 18 15912376
2014 Congenital mydriasis and prune belly syndrome in a child with an ACTA2 mutation. Journal of AAPOS : the official publication of the American Association for Pediatric Ophthalmology and Strabismus 17 24998021
2007 Understanding h-prune biology in the fight against cancer. Clinical & experimental metastasis 17 17952613
1997 Prune-belly syndrome and other anomalies in a stillborn fetus with a ring X chromosome lacking XIST. American journal of medical genetics 17 9129738
1992 Tricho-rhino-phalangeal syndrome type II (Langer-Giedion) with persistent cloaca and prune belly sequence in a girl with 8q interstitial deletion. American journal of medical genetics 17 1481848
1986 An association of prune belly anomaly with trisomy 21. American journal of medical genetics 17 2938473
2021 NMIHBA results from hypomorphic PRUNE1 variants that lack short-chain exopolyphosphatase activity. Human molecular genetics 16 33105479
2014 A therapeutic approach to treat prostate cancer by targeting Nm23-H1/h-Prune interaction. Naunyn-Schmiedeberg's archives of pharmacology 16 25138575
2007 Domain mapping on the human metastasis regulator protein h-Prune reveals a C-terminal dimerization domain. The Biochemical journal 16 17655525
2006 Prune extract (Prunus domestica L.) suppresses the proliferation and induces the apoptosis of human colon carcinoma Caco-2. Journal of nutritional science and vitaminology 16 17190111
2004 Unraveling genes and pathways influenced by H-prune PDE overexpression: a model to study cellular motility. Cell cycle (Georgetown, Tex.) 16 15254413
2004 Immunodiagnosis of Prune dwarf virus using antiserum produced to its recombinant coat protein. Journal of virological methods 16 15350730
2012 Novel pyrimidopyrimidine derivatives for inhibition of cellular proliferation and motility induced by h-prune in breast cancer. European journal of medicinal chemistry 15 23059542
2002 Use of genetically engineered mice in drug discovery and development: wielding Occam's razor to prune the product portfolio. International journal of toxicology 15 11936900
1997 The complete nucleotide sequence of prune dwarf ilarvirus RNA-1. Archives of virology 15 9672650
1991 Morphology and histochemistry of infant testes in the prune belly syndrome. The Journal of urology 15 1682513
2017 Rare copy number variants identified in prune belly syndrome. European journal of medical genetics 14 29174092
2023 Human Prune Regulates the Metabolism of Mammalian Inorganic Polyphosphate and Bioenergetics. International journal of molecular sciences 13 37762163
2013 Mapping functional interaction sites of human prune C-terminal domain by NMR spectroscopy in human cell lysates. Chemistry (Weinheim an der Bergstrasse, Germany) 13 23939913
1975 Temperature-sensitive prune (pn) mutations of Drosophila melanogaster. Mutation research 13 814448
2024 L-Glutamate treatment alleviates chilling injury of prune (Prunus domestica L.) fruit by regulating ROS homeostasis, GABA shunt, and energy metabolism. Food chemistry 12 39208637
2022 Prune supplementation for 12 months alters the gut microbiome in postmenopausal women. Food & function 12 36350082
2021 Functional Genomics of PRUNE1 in Neurodevelopmental Disorders (NDDs) Tied to Medulloblastoma (MB) and Other Tumors. Frontiers in oncology 12 34745995
2018 PRUNE1-related disorder: Expanding the clinical spectrum. Clinical genetics 12 29797509
2017 Lost in space? Generalising subtree prune and regraft to spaces of phylogenetic networks. Journal of theoretical biology 12 28414085
2018 PRUNE1 Deficiency: Expanding the Clinical and Genetic Spectrum. Neuropediatrics 11 29940663
2018 Ultrastructural Analysis of Prune DwarfVirus Intercellular Transport and Pathogenesis. International journal of molecular sciences 11 30158483
2018 A homozygous canonical splice acceptor site mutation in PRUNE1 is responsible for a rare childhood neurodegenerative disease in Manitoba Cree families. American journal of medical genetics. Part A 11 30556349
2017 Spinal motor neuron involvement in a patient with homozygous PRUNE mutation. European journal of paediatric neurology : EJPN : official journal of the European Paediatric Neurology Society 11 29307700
2011 PRUNE and PROBE--two modular web services for protein-protein docking. Nucleic acids research 11 21576226
2004 Carbohydrate composition of selected plum/prune preparations. Journal of agricultural and food chemistry 11 14969541
2003 Cellular Location of Prune dwarf virus in Almond Sections by In Situ Reverse Transcription-Polymerase Chain Reaction. Phytopathology 11 18944337
2018 PRUNE Syndrome Is a New Neurodevelopmental Disorder: Report and Review. Child neurology open 10 29372174
2011 Prune melanoidins protect against oxidative stress and endothelial cell death. Frontiers in bioscience (Elite edition) 10 21622112
2009 Detection of Prune dwarf virus by one-step RT-PCR and its quantitation by real-time PCR. Journal of virological methods 10 20003914
2005 Loss-of-function mutations in a glutathione S-transferase suppress the prune-Killer of prune lethal interaction. Genetics 10 16143620
2004 Giant omphalocele and "prune belly" sequence as components of the Beckwith-Wiedemann syndrome. American journal of medical genetics. Part A 10 15316976
2024 PRUNE1 and NME/NDPK family proteins influence energy metabolism and signaling in cancer metastases. Cancer metastasis reviews 9 38180572
2024 Salicylic Acid Treatment Ameliorates Postharvest Quality Deterioration in 'France' Prune (Prunus domestica L. 'Ximei') Fruit by Modulating the Antioxidant System. Foods (Basel, Switzerland) 9 39335799
2020 Modifications in Tissue and Cell Ultrastructure as Elements of Immunity-Like Reaction in Chenopodium quinoa against Prune Dwarf Virus (PDV). Cells 9 31936247
2019 Integrative analysis of h-prune as a potential therapeutic target for hepatocellular carcinoma. EBioMedicine 9 30665854
2018 The Incidence and Genetic Diversity of Apple Mosaic Virus (ApMV) and Prune Dwarf Virus (PDV) in Prunus Species in Australia. Viruses 9 29562672
2016 h-prune affects anaplastic thyroid cancer invasion and metastasis. Oncology reports 9 27109060
2011 Hepatoblastoma and prune belly syndrome: a potential association. Pediatric nephrology (Berlin, Germany) 9 21597971
2010 Prune belly syndrome associated with cloacal anomaly, patent urachal remnant, and omphalocele in a female infant. Journal of pediatric surgery 9 21034928
2005 Genomic variability of prune dwarf virus as affected by agricultural practice. Archives of virology 9 15789267
1994 The mRNA product of the Drosophila gene prune is spliced and encodes a protein containing a putative transmembrane domain. Molecular & general genetics : MGG 9 8121405
1986 Prune belly syndrome and retroperitoneal germ cell tumor. The American journal of medicine 9 2877580
2020 Inoculum quantification of canker-causing pathogens in prune and walnut orchards using real-time PCR. Journal of applied microbiology 8 32406554
2019 Altered MR imaging findings in a Japanese female child with PRUNE1-related disorder. Brain & development 8 31882333
2014 Development and Practical Use of RT-PCR for Seed-transmitted Prune dwarf virus in Quarantine. The plant pathology journal 8 25289000
2012 Spatial and temporal expressions of prune reveal a role in Müller gliogenesis during Xenopus retinal development. Gene 8 22967741
2023 Tumor Environment Regression Therapy Implemented by Switchable Prune-to-Essence Nanoplatform Unleashed Systemic Immune Responses. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 7 37875395
2022 Neurodevelopmental disorder with microcephaly, hypotonia, and variable brain anomalies in a consanguineous Iranian family is associated with a homozygous start loss variant in the PRUNE1 gene. BMC medical genomics 7 35379233
2021 Molecular Identification of Prune Dwarf Virus (PDV) Infecting Sweet Cherry in Canada and Development of a PDV Full-Length Infectious cDNA Clone. Viruses 7 34696454
2017 Molecular Biology of Prune Dwarf Virus-A Lesser Known Member of the Bromoviridae but a Vital Component in the Dynamic Virus-Host Cell Interaction Network. International journal of molecular sciences 7 29258199
1988 [Prune belly syndrome. Prenatal diagnosis and obstetric procedure]. Zeitschrift fur Geburtshilfe und Perinatologie 7 2975088

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