| 2022 |
CircLRFN5 binds directly to PRRX2 protein and promotes its degradation via a ubiquitin-mediated proteasomal pathway; PRRX2 transcriptionally upregulates GCH1 expression in glioma stem cells, suppressing ferroptosis by generating the antioxidant tetrahydrobiopterin (BH4). |
RNA immunoprecipitation, RNA pull-down assay, ubiquitination assay, dual-luciferase reporter assay, chromatin immunoprecipitation assay, shRNA knockdown/overexpression in glioma stem cells |
Journal of experimental & clinical cancer research : CR |
Medium |
36266731
|
| 2021 |
PRRX2 functions as a transcription factor for IL-6, and CBS-mediated suppression of PRRX2 reduces IL-6/STAT3 pathway activity, leading to reduced Treg infiltration and increased apoptosis in hepatocellular carcinoma. |
HCC cell line overexpression/knockdown, in vivo xenograft and Cbs heterozygous knockout mouse models, signaling pathway analysis |
Journal for immunotherapy of cancer |
Medium |
34413167
|
| 2017 |
PRRX2 (Prrx2) is a direct target of miR-212-5p; miR-212-5p suppresses Prrx2 by binding its 3'-UTR, and Prrx2 overexpression partially rescues miR-212-5p-mediated suppression of EMT in triple-negative breast cancer cells. |
Luciferase reporter assay targeting 3'-UTR, Western blot, wound healing and Transwell invasion assays, overexpression rescue experiments |
Cellular physiology and biochemistry |
Medium |
29216628
|
| 2017 |
Knockdown of Prrx2 induces mesenchymal-to-epithelial transition in breast cancer cells, prevents nuclear translocation of β-catenin, and inhibits Wnt/β-catenin signaling, suppressing invasion and migration in vitro and tumor growth in vivo. |
shRNA knockdown in breast cancer cell lines, Western blot for EMT and Wnt/β-catenin markers, in vitro invasion/migration assays, nude mouse xenograft |
Cellular physiology and biochemistry |
Medium |
28750408
|
| 2019 |
PRRX2 knockdown in colon cancer cells inactivates Wnt/β-catenin signaling (reducing p-GSK3βSer9/GSK3β, nuclear/cytoplasmic β-catenin, TCF4, and Vimentin; increasing E-cadherin), suppresses invasion and migration in vitro, and reduces liver metastasis in vivo; the Wnt activator LiCl reverses the inhibitory effect of PRRX2 silencing. |
siRNA/shRNA knockdown in CT-26 and CMT93 colon cancer cells, Western blot, Transwell invasion/migration assay, liver-metastatic mouse model, LiCl pathway rescue |
Pathology, research and practice |
Medium |
31471104
|
| 2022 |
PRRX2 activation in prostate cancer cells promotes enzalutamide resistance through alterations in the CDK4/6/Rb/E2F and BCL2 pathways, identified via a genome-wide CRISPR activation screen. |
Genome-wide CRISPRa screen in metastatic castration-sensitive prostate cancer cells, pathway analysis of CDK4/6/Rb/E2F and BCL2, CDK4/6 and BCL2 inhibitor treatment |
Cancer research |
Medium |
35405009
|
| 2023 |
Myoblast-derived exosomal PRRX2 directly binds to the MIR22HG promoter and promotes its transcription and expression; MIR22HG then sponges miR-128 to enhance YAP nuclear translocation, thereby facilitating osteogenic differentiation of BMSCs. |
Dual-luciferase reporter assay, RIP assay, ChIP assay, immunofluorescence for YAP nuclear translocation, exosome isolation and delivery, siRNA knockdown, overexpression rescue in BMSCs, ovariectomy mouse model |
Molecular medicine |
Medium |
37081396
|
| 2014 |
KLF6 specifically stimulates Prrx2 expression; KLF6 can bind to the Prrx2 promoter as shown by EMSA, and siRNA-mediated KLF6 knockdown reduces Prrx2 expression in pituitary cells. |
Promoter luciferase assay, electrophoretic mobility shift assay (EMSA), siRNA knockdown, immunohistochemistry |
The Journal of reproduction and development |
Medium |
24881871
|
| 2019 |
Attenuation of PRRX2 (together with HEY2) significantly enhances the transdifferentiation of adult human skin fibroblasts to induced neurons when combined with ASCL1 overexpression and p53 knockdown, indicating PRRX2 functions as a barrier in the fibroblast gene regulatory network that suppresses neural fate acquisition. |
shRNA knockdown of PRRX2 in human skin fibroblasts, RNAseq comparison of skin vs lung fibroblasts during neuronal conversion, MAP2+ neuron quantification |
Biochemical and biophysical research communications |
Medium |
31255287
|
| 2024 |
PRRX2 directly binds to the ATOX1 promoter and positively regulates ATOX1 transcription in hepatocellular carcinoma cells; PRRX2 knockdown inhibits cell proliferation, invasion, and EMT while promoting cuproptosis, effects partially reversed by ATOX1 overexpression. |
ChIP assay, dual-luciferase reporter assay, JASPAR-predicted binding site analysis, siRNA knockdown, overexpression rescue in HCC cell lines, xenograft model |
Cellular signalling |
Medium |
40393577
|
| 2024 |
PRRX2 directly binds to the Panx3 promoter and positively regulates Panx3 expression in preodontoblasts; PRRX2 knockdown with siRNA inhibits Panx3 expression, and PRRX2 overexpression in HEK293 cells increases luciferase activity from a Panx3 promoter reporter. |
Dual-luciferase reporter assay, electrophoretic mobility shift assay (EMSA), siRNA knockdown in dental papilla-derived cells |
Journal of oral biosciences |
Medium |
39733924
|
| 2025 |
PRRX2 directly enhances transcription of GLI2, activating the Hedgehog pathway; this inhibits tumor cell senescence and promotes proliferation, invasion, and metastasis in pancreatic cancer; Hedgehog pathway inhibitors or GLI2 silencing partially reverse PRRX2's oncogenic effects. |
ChIP/luciferase (implied by 'directly enhanced transcription'), shRNA knockdown and overexpression in pancreatic cancer cells, senescence assays, Hedgehog pathway inhibitor treatment, GLI2 silencing rescue |
Cancer science |
Low |
40619170
|
| 2025 |
PRRX2 binds to the CNTN3 promoter and transcriptionally activates CNTN3 in colorectal cancer; PRRX2 knockdown reduces CNTN3 expression, reverses EMT markers, and suppresses metastatic phenotypes; rescue with CNTN3 overexpression restores these effects. |
Chromatin immunoprecipitation (ChIP), dual-luciferase reporter assay, shRNA knockdown and rescue overexpression in CRC cell lines, xenograft model |
Cell division |
Medium |
41882765
|
| 2025 |
PRRX2 amplifies TGF-β signaling through transcriptional activation of TGFB1 and activates Fibronectin 1 to remodel the ECM in pancreatic ductal adenocarcinoma; PRRX2 knockdown reduces tumor cell migration in vitro. |
Single-cell RNA-seq and spatial transcriptomics, cell-to-cell communication network analysis, shRNA knockdown in PDAC cell lines, multicolor immunofluorescence staining |
Chinese medical journal |
Low |
41456929
|
| 2020 |
Silencing Prrx2 in breast cancer cells inhibits nuclear β-catenin expression and downregulates Wnt/β-catenin signaling activity, suppressing cell proliferation in vitro and tumor growth in vivo. |
shRNA knockdown in MDA-MB-231 and MCF-7 cells, MTT proliferation assay, nude mouse xenograft, Western blot for β-catenin |
Zhonghua yi xue za zhi |
Low |
32234171
|
| 2024 |
Silencing of Marveld3 leads to upregulation of PRRX2, which subsequently reduces intracellular Fe2+ and ROS levels and suppresses lipid peroxidation, thereby attenuating radiation-induced ferroptosis in skin cells. |
siRNA silencing of Marveld3, PRRX2 overexpression/siRNA in HaCaT and WS1 cells, MDA/Fe2+/ROS assays, BODIPY staining, RNA sequencing |
Molecular medicine |
Low |
39434056
|