Affinage

PRPF6

Pre-mRNA-processing factor 6 · UniProt O94906

Round 2 corrected
Length
941 aa
Mass
106.9 kDa
Annotated
2026-04-28
130 papers in source corpus 8 papers cited in narrative 8 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PRPF6 is a U5 snRNP-associated protein that serves as the principal bridging factor for assembly of the U4/U6.U5 tri-snRNP, a key intermediate required for spliceosome formation and pre-mRNA splicing. It associates with U4, U5, and U6 snRNAs and connects the U4/U6 di-snRNP to U5 snRNP by binding the U4/U6-associated 61K protein; loss of PRPF6 function selectively depletes tri-snRNP while leaving the U4/U6 di-snRNP intact (PMID:8479905, PMID:15840814). A conserved missense mutation (p.Arg729Trp) causes autosomal-dominant retinitis pigmentosa by impairing tri-snRNP assembly or recycling, leading to aberrant Cajal body accumulation and intron retention in patient cells (PMID:21549338). PRPF6 also supports cancer cell proliferation through tri-snRNP-dependent preferential splicing of growth-regulatory genes including an oncogenic ZAK kinase isoform (PMID:24788092).

Mechanistic history

Synthesis pass · year-by-year structured walk · 6 steps
  1. 1990 High

    Establishing that PRP6 is a snRNP-associated protein required for splicing resolved whether this gene product acts at the level of snRNP function rather than at other steps of mRNA metabolism.

    Evidence Immunoprecipitation of U4/U5/U6 snRNAs with PRP6 antibodies and in vitro splicing defects in temperature-sensitive yeast mutant extracts

    PMID:2118103 PMID:2147224

    Open questions at the time
    • Whether PRP6 is a component of the U4/U6 di-snRNP or specifically of the tri-snRNP was not resolved
    • The direct protein-protein contacts mediating PRP6 function were unknown
    • No human ortholog had been characterized
  2. 1993 High

    Demonstrating that PRP6 loss selectively depletes the tri-snRNP while sparing the U4/U6 di-snRNP established PRP6 as a tri-snRNP-specific assembly factor rather than a general snRNP component.

    Evidence Glycerol gradient sedimentation of snRNP complexes from heat-inactivated prp6-1 yeast extracts combined with antibody co-immunoprecipitation

    PMID:8479905

    Open questions at the time
    • The molecular mechanism by which PRP6 bridges di-snRNP to U5 snRNP was unknown
    • Whether this role is conserved in human PRPF6 had not been tested
  3. 2005 High

    Identifying human PRPF6 as the bridging protein that connects U4/U6 di-snRNP (via the 61K protein) to U5 snRNP defined the molecular mechanism of tri-snRNP assembly in human cells.

    Evidence Yeast two-hybrid screening, GST pulldown, and mass spectrometry with human spliceosomal proteins

    PMID:15840814

    Open questions at the time
    • Structural basis of the PRPF6–61K interaction was not determined
    • Whether PRPF6 has functions beyond tri-snRNP bridging was unclear
  4. 2011 High

    Linking a conserved PRPF6 missense mutation (p.Arg729Trp) to autosomal-dominant retinitis pigmentosa established that impaired tri-snRNP assembly causes tissue-specific human disease, with Cajal body accumulation serving as a cytological marker of the defect.

    Evidence Mutation screening in RP families, immunofluorescence of endogenous and GFP-tagged PRPF6 in patient lymphoblasts and HeLa cells, intron retention analysis

    PMID:21549338

    Open questions at the time
    • Why retinal photoreceptors are selectively vulnerable to a general splicing defect was not explained
    • The specific pre-mRNA targets whose mis-splicing leads to retinal degeneration were not identified
  5. 2012 Medium

    Proteomic and systematic interaction mapping placed PRPF6 within the broader spliceosomal protein network and confirmed its presence in functional human spliceosomes.

    Evidence Spliceosome affinity purification with LC-MS/MS and comprehensive Y2H/co-IP interaction matrix

    PMID:12226669 PMID:22365833

    Open questions at the time
    • PRPF6-specific interactions were not individually characterized in mechanistic detail
    • Dynamic rearrangement of PRPF6 contacts across spliceosome catalytic states was not resolved
  6. 2014 High

    Demonstrating that PRPF6 knockdown selectively impairs cancer cell growth and alters splicing of oncogenic gene isoforms (including ZAK kinase) revealed a tri-snRNP-specific vulnerability in tumors with high tri-snRNP expression.

    Evidence RNAi knockdown, RNA-seq transcriptome analysis, and cancer cell proliferation assays

    PMID:24788092

    Open questions at the time
    • Whether PRPF6 overexpression is a driver or passenger event in cancer initiation was not established
    • The mechanism by which PRPF6 levels dictate splicing outcome for specific transcripts was not resolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • A high-resolution structural model of PRPF6 within the tri-snRNP and the basis for its substrate-specific splicing effects remain to be determined.
  • No atomic-resolution structure of PRPF6 in the context of the tri-snRNP has been reported in this timeline
  • The mechanism by which PRPF6 levels preferentially influence alternative splicing of specific pre-mRNAs is unresolved
  • Why mutations in a ubiquitous splicing factor cause retina-specific disease is unexplained

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0003723 RNA binding 3 GO:0005198 structural molecule activity 2
Localization
GO:0005654 nucleoplasm 2 GO:0005634 nucleus 1
Pathway
R-HSA-8953854 Metabolism of RNA 4 R-HSA-1643685 Disease 2
Partners
CD2BP2PRPF31
Complex memberships
U4/U6.U5 tri-snRNPU5 snRNP

Evidence

Reading pass · 8 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1990 Yeast PRP6 encodes a U4/U6 snRNP-associated protein required for pre-mRNA splicing; immunoprecipitation of PRP6 protein co-precipitates U4, U5, and U6 snRNAs, and in vitro splicing extracts from temperature-sensitive prp6 mutants are defective in spliceosome assembly, placing PRP6 at the level of snRNP function. Immunoprecipitation of snRNAs, in vitro splicing assay with temperature-sensitive mutant extracts, spliceosome assembly analysis Molecular and cellular biology High 2147224
1990 The yeast PRP6 protein (899 amino acids) contains repeated helix-loop-helix motifs and cysteine/histidine motifs related to zinc fingers; directed mutagenesis of specific cysteine/histidine residues abolishes protein function, and PRP6 contains leucine repeat motifs implicated in protein-protein interactions. Temperature-sensitive prp6 mutations were mapped and sequenced, establishing structure-function relationships. DNA sequencing, directed mutagenesis of cysteine/histidine residues, genetic complementation The EMBO journal High 2118103
1993 Antibodies against yeast PRP6 co-precipitate U4, U5, and U6 snRNAs. In heat-inactivated prp6-1 mutant extracts, the [U4/U6.U5] tri-snRNP is present in very low amounts while U4/U6 di-snRNP particles remain normally represented, establishing that PRP6 is specifically required for accumulation of the tri-snRNP but is not a component of the U4/U6 di-snRNP. Antibody co-immunoprecipitation of snRNAs, glycerol gradient sedimentation analysis of snRNP complexes from temperature-sensitive mutant extracts Nucleic acids research High 8479905
2005 Human PRPF6 (U5-102K/hPrp6) is a component of the 20S U5 snRNP and functions as a bridging protein essential for U4/U6.U5 tri-snRNP formation by binding the U4/U6-associated 61K protein; it also interacts with the U5-specific 52K protein (CD2BP2) through its N-terminal region, but this interaction is lost upon tri-snRNP assembly, suggesting a role for 52K in tri-snRNP assembly rather than as a stable tri-snRNP component. Yeast two-hybrid screening, GST pulldown assays, mass spectrometry protein identification, immunofluorescence localization RNA (New York, N.Y.) High 15840814
2011 A missense mutation c.2185C>T (p.Arg729Trp) in PRPF6, affecting a residue conserved from humans to yeast, causes autosomal-dominant retinitis pigmentosa. Patient lymphoblasts show abnormal nuclear localization of endogenous PRPF6 with accumulation in Cajal bodies, indicating impaired tri-snRNP assembly or recycling. GFP-tagged mutant PRPF6 expressed in HeLa cells recapitulates the Cajal body accumulation phenotype. Patient cells display intron retention for pre-mRNAs bearing specific splicing signals, consistent with a splicing defect. Mutation screening by sequencing, immunofluorescence localization of endogenous and GFP-tagged PRPF6 in patient lymphoblasts and HeLa cells, endogenous transcript analysis for intron retention American journal of human genetics High 21549338
2014 PRPF6 drives cancer cell proliferation through preferential splicing of growth-regulatory genes. Inhibition of PRPF6 and other tri-snRNP complex proteins, but not other snRNP complexes, selectively abrogates growth in cancer cells with high tri-snRNP levels. Reduced PRPF6 alters constitutive and alternative splicing of a discrete set of genes, including production of an oncogenic isoform of the ZAK kinase, establishing PRPF6 as a tri-snRNP-specific splicing factor with oncogenic roles. RNAi knockdown, high-resolution transcriptome analysis (RNA-seq), cancer cell proliferation assays, isoform-specific splicing analysis Genes & development High 24788092
2012 PRPF6 was identified as a component of the human spliceosome through comprehensive proteomic analysis; it is present in purified functional spliceosomes along with other U5 snRNP proteins. Affinity chromatography purification of spliceosomes, nanoscale LC-MS/MS mass spectrometry Nature Medium 12226669
2012 Systematic yeast two-hybrid and co-immunoprecipitation analyses of the human spliceosomal protein interaction network identified specific protein-protein interactions involving hPrp6 (PRPF6) within the tri-snRNP complex, contributing to the dynamic wiring map of the spliceosome across its assembly states. Comprehensive Y2H interaction matrix, co-immunoprecipitation validation, integration with spliceosomal complex purification data Molecular cell Medium 22365833

Source papers

Stage 0 corpus · 130 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2010 The nuclear-retained noncoding RNA MALAT1 regulates alternative splicing by modulating SR splicing factor phosphorylation. Molecular cell 1776 20797886
2012 Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. Cell 1718 22658674
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2009 Defining the human deubiquitinating enzyme interaction landscape. Cell 1282 19615732
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2017 Architecture of the human interactome defines protein communities and disease networks. Nature 1085 28514442
2015 A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 1015 26496610
2018 VIRMA mediates preferential m6A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell discovery 829 29507755
2008 Global analysis of host-pathogen interactions that regulate early-stage HIV-1 replication. Cell 787 18854154
2003 Complete sequencing and characterization of 21,243 full-length human cDNAs. Nature genetics 754 14702039
2007 Large-scale mapping of human protein-protein interactions by mass spectrometry. Molecular systems biology 733 17353931
2002 Comprehensive proteomic analysis of the human spliceosome. Nature 725 12226669
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2018 High-Density Proximity Mapping Reveals the Subcellular Organization of mRNA-Associated Granules and Bodies. Molecular cell 580 29395067
2012 Role of PINK1 binding to the TOM complex and alternate intracellular membranes in recruitment and activation of the E3 ligase Parkin. Developmental cell 534 22280891
2017 Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. Science (New York, N.Y.) 533 28302793
1994 Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. Gene 492 8125298
2013 Identification of 23 new prostate cancer susceptibility loci using the iCOGS custom genotyping array. Nature genetics 463 23535732
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2010 Systematic analysis of human protein complexes identifies chromosome segregation proteins. Science (New York, N.Y.) 421 20360068
2015 Panorama of ancient metazoan macromolecular complexes. Nature 407 26344197
2005 The human SWI/SNF subunit Brm is a regulator of alternative splicing. Nature structural & molecular biology 379 16341228
2007 Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Molecular cell 367 17643375
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2012 Dynamic protein-protein interaction wiring of the human spliceosome. Molecular cell 318 22365833
2018 Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems. Science China. Life sciences 312 30446870
2013 Endogenous purification reveals GREB1 as a key estrogen receptor regulatory factor. Cell reports 307 23403292
2016 Identification of Zika Virus and Dengue Virus Dependency Factors using Functional Genomics. Cell reports 306 27342126
2002 Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. RNA (New York, N.Y.) 301 11991638
2005 Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell 281 15797382
1999 The TOM core complex: the general protein import pore of the outer membrane of mitochondria. The Journal of cell biology 173 10579717
2019 Cryo-EM structure of the mitochondrial protein-import channel TOM complex at near-atomic resolution. Nature structural & molecular biology 149 31740857
2001 Protein import channel of the outer mitochondrial membrane: a highly stable Tom40-Tom22 core structure differentially interacts with preproteins, small tom proteins, and import receptors. Molecular and cellular biology 145 11259583
2006 Genetic and physiological characterization of tomato cv. Micro-Tom. Journal of experimental botany 143 16687436
2017 Cryo-EM Structure of the TOM Core Complex from Neurospora crassa. Cell 141 28802041
2001 Tom40, the pore-forming component of the protein-conducting TOM channel in the outer membrane of mitochondria. The Journal of cell biology 140 11402060
2006 Cytosolic factor- and TOM-independent import of C-tail-anchored mitochondrial outer membrane proteins. The EMBO journal 139 17110923
2001 Biogenesis of porin of the outer mitochondrial membrane involves an import pathway via receptors and the general import pore of the TOM complex. The Journal of cell biology 134 11266446
2010 Large-scale analysis of full-length cDNAs from the tomato (Solanum lycopersicum) cultivar Micro-Tom, a reference system for the Solanaceae genomics. BMC genomics 132 20350329
2003 Mitochondrial translocation contact sites: separation of dynamic and stabilizing elements in formation of a TOM-TIM-preprotein supercomplex. The EMBO journal 131 14532110
1999 Biogenesis of Tom40, core component of the TOM complex of mitochondria. The Journal of cell biology 128 10427088
2007 Biogenesis of the mitochondrial TOM complex: Mim1 promotes insertion and assembly of signal-anchored receptors. The Journal of biological chemistry 124 17974559
2011 Tomato TILLING technology: development of a reverse genetics tool for the efficient isolation of mutants from Micro-Tom mutant libraries. Plant & cell physiology 111 21965606
2011 A missense mutation in PRPF6 causes impairment of pre-mRNA splicing and autosomal-dominant retinitis pigmentosa. American journal of human genetics 106 21549338
2020 Atomic structure of human TOM core complex. Cell discovery 104 33083003
2002 Protein translocase of the outer mitochondrial membrane: role of import receptors in the structural organization of the TOM complex. Journal of molecular biology 104 11866524
2009 Salinity induces carbohydrate accumulation and sugar-regulated starch biosynthetic genes in tomato (Solanum lycopersicum L. cv. 'Micro-Tom') fruits in an ABA- and osmotic stress-independent manner. Journal of experimental botany 103 19995825
1990 The yeast PRP6 gene encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein, and the PRP9 gene encodes a protein required for U2 snRNP binding. Molecular and cellular biology 103 2147224
2007 Alternative function for the mitochondrial SAM complex in biogenesis of alpha-helical TOM proteins. The Journal of cell biology 100 18039934
1997 The Tom and Tim machine. Current biology : CB 97 9081657
2009 Profiling of melatonin in the model tomato (Solanum lycopersicum L.) cultivar Micro-Tom. Journal of pineal research 96 19317796
2007 The transmembrane segment of Tom20 is recognized by Mim1 for docking to the mitochondrial TOM complex. Journal of molecular biology 92 18187149
2022 Role of the TOM Complex in Protein Import into Mitochondria: Structural Views. Annual review of biochemistry 85 35287471
2021 Mechanism of PINK1 activation by autophosphorylation and insights into assembly on the TOM complex. Molecular cell 84 34875213
2004 Tom22', an 8-kDa trans-site receptor in plants and protozoans, is a conserved feature of the TOM complex that appeared early in the evolution of eukaryotes. Molecular biology and evolution 84 15155803
2009 Mitochondrial targeting of tBid/Bax: a role for the TOM complex? Cell death and differentiation 79 19521421
1997 Immune suppression and Th1/Th2 balance in pregnancy revisited: a (very) personal tribute to Tom Wegmann. American journal of reproductive immunology (New York, N.Y. : 1989) 78 9228297
2014 An integrative analysis of colon cancer identifies an essential function for PRPF6 in tumor growth. Genes & development 77 24788092
1990 The molecular characterization of PRP6 and PRP9 yeast genes reveals a new cysteine/histidine motif common to several splicing factors. The EMBO journal 77 2118103
2018 Recruitment of Cytosolic J-Proteins by TOM Receptors Promotes Mitochondrial Protein Biogenesis. Cell reports 73 30463002
2006 The enteropathogenic Escherichia coli (EPEC) Map effector is imported into the mitochondrial matrix by the TOM/Hsp70 system and alters organelle morphology. Cellular microbiology 73 16548893
2012 Evaluation of four Agrobacterium tumefaciens strains for the genetic transformation of tomato (Solanum lycopersicum L.) cultivar Micro-Tom. Plant cell reports 72 23099543
2003 Arabidopsis TOBAMOVIRUS MULTIPLICATION (TOM) 2 locus encodes a transmembrane protein that interacts with TOM1. The EMBO journal 72 12514139
2016 Ground control to major TOM: mitochondria-nucleus communication. The FEBS journal 71 27283924
2015 Sam37 is crucial for formation of the mitochondrial TOM-SAM supercomplex, thereby promoting β-barrel biogenesis. The Journal of cell biology 71 26416958
2001 Apocytochrome c requires the TOM complex for translocation across the mitochondrial outer membrane. The EMBO journal 71 11598006
2006 Dorsal-ventral pattern of Delta trafficking is established by a Snail-Tom-Neuralized pathway. Developmental cell 69 16459304
2000 Recognition of preproteins by the isolated TOM complex of mitochondria. The EMBO journal 67 10990453
2005 Mim1, a protein required for the assembly of the TOM complex of mitochondria. EMBO reports 66 15608614
2002 Bcl-2 and porin follow different pathways of TOM-dependent insertion into the mitochondrial outer membrane. Journal of molecular biology 65 12419260
2007 The mitochondrial TOM complex is required for tBid/Bax-induced cytochrome c release. The Journal of biological chemistry 64 17635912
1998 Dynamics of the TOM complex of mitochondria during binding and translocation of preproteins. Molecular and cellular biology 62 9710610
2002 Biogenesis of the mitochondrial TOM complex. Trends in biochemical sciences 58 11943546
2009 Cloning and characterization of a Chlamydomonas reinhardtii cDNA arylalkylamine N-acetyltransferase and its use in the genetic engineering of melatonin content in the Micro-Tom tomato. Journal of pineal research 56 19552760
2001 Assembly of Tom6 and Tom7 into the TOM core complex of Neurospora crassa. The Journal of biological chemistry 55 11278536
2024 Tom20 gates PINK1 activity and mediates its tethering of the TOM and TIM23 translocases upon mitochondrial stress. Proceedings of the National Academy of Sciences of the United States of America 54 38416681
2021 Mapping protein interactions in the active TOM-TIM23 supercomplex. Nature communications 53 34588454
2022 Structural basis of Tom20 and Tom22 cytosolic domains as the human TOM complex receptors. Proceedings of the National Academy of Sciences of the United States of America 52 35733257
2011 Lateral release of proteins from the TOM complex into the outer membrane of mitochondria. The EMBO journal 51 21765393
2020 From TOM to the TIM23 complex - handing over of a precursor. Biological chemistry 50 32074073
2014 Cooperation of TOM and TIM23 complexes during translocation of proteins into mitochondria. Journal of molecular biology 50 25083920
2005 Role of Tom5 in maintaining the structural stability of the TOM complex of mitochondria. The Journal of biological chemistry 50 15701639
2005 The human U5 snRNP 52K protein (CD2BP2) interacts with U5-102K (hPrp6), a U4/U6.U5 tri-snRNP bridging protein, but dissociates upon tri-snRNP formation. RNA (New York, N.Y.) 50 15840814
2003 Comparison of the TIM and TOM channel activities of the mitochondrial protein import complexes. Biophysical journal 50 12719229
1994 The Drosophila tom retrotransposon encodes an envelope protein. Molecular and cellular biology 50 8035817
2023 Molecular pathway of mitochondrial preprotein import through the TOM-TIM23 supercomplex. Nature structural & molecular biology 49 37696957
2003 Targeting and assembly of mitochondrial tail-anchored protein Tom5 to the TOM complex depend on a signal distinct from that of tail-anchored proteins dispersed in the membrane. The Journal of biological chemistry 47 12896971
1996 Identification of a third yeast mitochondrial Tom protein with tetratrico peptide repeats. FEBS letters 46 8612740
1988 Retrovirus-like features and site specific insertions of a transposable element, tom, in Drosophila ananassae. Molecular & general genetics : MGG 46 2851093
1993 The biochemical defects of prp4-1 and prp6-1 yeast splicing mutants reveal that the PRP6 protein is required for the accumulation of the [U4/U6.U5] tri-snRNP. Nucleic acids research 44 8479905
2013 Genome-wide analysis of intraspecific DNA polymorphism in 'Micro-Tom', a model cultivar of tomato (Solanum lycopersicum). Plant & cell physiology 43 24319074
2017 Major Tom to Ground Control: How Lipoproteins Communicate Extracytoplasmic Stress to the Decision Center of the Cell. Journal of bacteriology 42 28674071
2002 A conserved proline residue is present in the transmembrane-spanning domain of Tom7 and other tail-anchored protein subunits of the TOM translocase. FEBS letters 42 11943179
2003 Mitochondrial protein import: recognition of internal import signals of BCS1 by the TOM complex. Molecular and cellular biology 41 12640110
2008 Localization of the Carnation Italian ringspot virus replication protein p36 to the mitochondrial outer membrane is mediated by an internal targeting signal and the TOM complex. BMC cell biology 40 18811953
2001 Structural requirements of Tom40 for assembly into preexisting TOM complexes of mitochondria. Molecular biology of the cell 39 11359915
2000 Functional analysis of human metaxin in mitochondrial protein import in cultured cells and its relationship with the Tom complex. Biochemical and biophysical research communications 38 11027586
2022 Mitochondrial protein translocation machinery: From TOM structural biogenesis to functional regulation. The Journal of biological chemistry 35 35346689
2009 TOM-independent complex formation of Bax and Bak in mammalian mitochondria during TNFalpha-induced apoptosis. Cell death and differentiation 35 19165229
2008 The outer membrane form of the mitochondrial protein Mcr1 follows a TOM-independent membrane insertion pathway. FEBS letters 35 18279676
2015 TOMATOMA Update: Phenotypic and Metabolite Information in the Micro-Tom Mutant Resource. Plant & cell physiology 34 26719120
2012 The tomato (Solanum lycopersicum cv. Micro-Tom) natural genetic variation Rg1 and the DELLA mutant procera control the competence necessary to form adventitious roots and shoots. Journal of experimental botany 34 22915742
2025 Structure of human PINK1 at a mitochondrial TOM-VDAC array. Science (New York, N.Y.) 33 40080546
2013 The PRP6-like splicing factor STA1 is involved in RNA-directed DNA methylation by facilitating the production of Pol V-dependent scaffold RNAs. Nucleic acids research 32 23877244
2003 Biogenesis of yeast mitochondrial cytochrome c: a unique relationship to the TOM machinery. Journal of molecular biology 32 12628251
2024 ATAD1 prevents clogging of TOM and damage caused by un-imported mitochondrial proteins. Cell reports 31 39024102
2010 Molecular detection of Babesia bovis and Babesia bigemina in white-tailed deer (Odocoileus virginianus) from Tom Green County in central Texas. Veterinary parasitology 31 21194841
2021 The receptor subunit Tom20 is dynamically associated with the TOM complex in mitochondria of human cells. Molecular biology of the cell 30 34347503
2013 Novel TPR-containing subunit of TOM complex functions as cytosolic receptor for Entamoeba mitosomal transport. Scientific reports 30 23350036
2004 Targeting and assembly of rat mitochondrial translocase of outer membrane 22 (TOM22) into the TOM complex. The Journal of biological chemistry 30 14985332
2018 Genome-wide transcriptomic analysis of BR-deficient Micro-Tom reveals correlations between drought stress tolerance and brassinosteroid signaling in tomato. Plant physiology and biochemistry : PPB 29 29723826
2012 Chloroplast β-barrel proteins are assembled into the mitochondrial outer membrane in a process that depends on the TOM and TOB complexes. The Journal of biological chemistry 29 22745120
2005 Expressed sequence tags from the laboratory-grown miniature tomato (Lycopersicon esculentum) cultivar Micro-Tom and mining for single nucleotide polymorphisms and insertions/deletions in tomato cultivars. Gene 29 15975739
2021 A Biochemical and Structural Understanding of TOM Complex Interactions and Implications for Human Health and Disease. Cells 28 34064787
2022 Towards a molecular mechanism underlying mitochondrial protein import through the TOM and TIM23 complexes. eLife 27 35674314
2019 Triplet-pore structure of a highly divergent TOM complex of hydrogenosomes in Trichomonas vaginalis. PLoS biology 27 30608924
2019 Growth performance and gastrointestinal responses in heavy Tom turkeys fed antibiotic free corn-soybean meal diets supplemented with multiple doses of a single strain Bacillus subtilis probiotic (DSM29784)1. Poultry science 27 31180117
2024 Mechanism of human PINK1 activation at the TOM complex in a reconstituted system. Science advances 26 38848361
2015 The pro-apoptotic BH3-only protein Bim interacts with components of the translocase of the outer mitochondrial membrane (TOM). PloS one 26 25875815
2006 Effect of mutations in Tom40 on stability of the translocase of the outer mitochondrial membrane (TOM) complex, assembly of Tom40, and import of mitochondrial preproteins. The Journal of biological chemistry 26 16757481
2009 The mitochondrial TOM complex modulates bax-induced apoptosis in Drosophila. Biochemical and biophysical research communications 22 19138672
2001 An interplay between the TOM complex and porin isoforms in the yeast Saccharomyces cerevisiae mitochondria. FEBS letters 22 11434918
2023 Two conformations of the Tom20 preprotein receptor in the TOM holo complex. Proceedings of the National Academy of Sciences of the United States of America 21 37579144
2022 The role of the individual TOM subunits in the association of PINK1 with depolarized mitochondria. Journal of molecular medicine (Berlin, Germany) 21 35391620
2022 Melatonin in Micro-Tom Tomato: Improved Drought Tolerance via the Regulation of the Photosynthetic Apparatus, Membrane Stability, Osmoprotectants, and Root System. Life (Basel, Switzerland) 20 36431057
2012 Investigating the genetic basis of theory of mind (ToM): the role of catechol-O-methyltransferase (COMT) gene polymorphisms. PloS one 19 23209597
2004 Processes underlying the upregulation of Tom proteins in S. cerevisiae mitochondria depleted of the VDAC channel. Journal of bioenergetics and biomembranes 18 15224968