Affinage

PRDX3

Thioredoxin-dependent peroxide reductase, mitochondrial · UniProt P30048

Length
256 aa
Mass
27.7 kDa
Annotated
2026-04-28
76 papers in source corpus 24 papers cited in narrative 24 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PRDX3 is a mitochondrial 2-Cys peroxiredoxin that functions as a principal scavenger of hydrogen peroxide and organic hydroperoxides, using a thioredoxin/thioredoxin-reductase/NADPH electron relay to reduce peroxides via its catalytic Cys-47, which forms an intermolecular disulfide with Cys-168 within a decameric toroidal assembly (PMID:9363753, PMID:12773537). PRDX3 is imported into both the mitochondrial matrix (via MPP/MIP cleavage) and the intermembrane space (via the IMP complex), is transcriptionally induced by c-Myc and YAP1–TEAD1, and is subject to extensive post-translational regulation including SIRT3-mediated deacetylation at K253, SIRT4-mediated deacetylation at K92, KAT2A-mediated succinylation at K84, TRIM39-mediated K48-linked ubiquitination at K73/K149, and PPT1-mediated depalmitoylation at C108 (PMID:39591905, PMID:12011429, PMID:38945958, PMID:31655428, PMID:40765819, PMID:40457625, PMID:38195664, PMID:41865945). During ferroptosis, lipid-peroxide-driven hyperoxidation of the catalytic cysteine triggers PRDX3 translocation from mitochondria to the plasma membrane, where it inhibits cystine uptake and amplifies ferroptotic cell death (PMID:37863053). Biallelic loss-of-function mutations in PRDX3 cause autosomal recessive spinocerebellar ataxia (SCAR32), with patient cells showing impaired mitochondrial respiration and elevated oxidative damage (PMID:33889951).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 1995 Medium

    The initial identification of SP-22 (PRDX3) as a mitochondrial radical scavenger established that this protein directly protects radical-sensitive enzymes from oxidative inactivation, raising the question of its enzymatic mechanism.

    Evidence In vitro radical-scavenging assay using Fe²⁺/DTT radical-generating system with enzyme activity protection readouts in bovine adrenal mitochondria

    PMID:7654218

    Open questions at the time
    • Catalytic mechanism unknown
    • Electron donor not identified
    • Single radical-generating system tested
  2. 1997 High

    Reconstitution of the three-component electron relay (thioredoxin reductase → mitochondrial thioredoxin → SP-22 → peroxide) resolved the catalytic mechanism by which PRDX3 reduces H₂O₂ and organic hydroperoxides, establishing it as a bona fide thioredoxin-dependent peroxidase.

    Evidence In vitro reconstitution with purified SP-22, mt-Trx, and NADPH-dependent reductase; NADPH oxidation spectrophotometry

    PMID:9363753

    Open questions at the time
    • Active-site residues not yet mapped
    • Quaternary structure unknown
    • Relative contribution versus glutathione peroxidase unclear
  3. 1999 Medium

    Demonstrating that PRDX3 is stress-inducible and required for cellular tolerance to oxidative injury extended its role from a constitutive enzyme to a regulated stress-response factor, including in vivo during myocardial infarction.

    Evidence Antisense knockdown in endothelial cells; oxidative stress tolerance assays; in vivo rat MI model

    PMID:9890990

    Open questions at the time
    • Transcriptional regulators driving induction not identified
    • Mechanism of stress sensing not defined
  4. 2002 High

    Identification of PRDX3 as a direct transcriptional target of c-Myc linked mitochondrial antioxidant capacity to oncogenic signaling, showing PRDX3 is required for Myc-driven transformation and mitochondrial membrane potential maintenance.

    Evidence ChIP at PRDX3 locus; MycER induction; knockdown/overexpression with transformation and mitochondrial imaging assays in rat and human cells

    PMID:12011429

    Open questions at the time
    • Whether Myc regulation is direct at the promoter or enhancer not fully resolved
    • Post-translational regulation by Myc pathway not examined
  5. 2003 High

    Structural and mutational analysis revealed that PRDX3 assembles as a decameric toroid and identified Cys-47 as the peroxidatic cysteine forming an intermolecular disulfide with Cys-168, defining the 2-Cys peroxiredoxin mechanism.

    Evidence Recombinant WT and Cys→Ser mutants; electron microscopy; circular dichroism; peroxidase activity assays

    PMID:12773537

    Open questions at the time
    • High-resolution atomic structure not available
    • Dynamics of dimer–decamer transitions under redox cycling not characterized
  6. 2007 High

    PRDX3 knockout mice demonstrated that PRDX3 is a non-redundant ROS scavenger in vivo, as loss causes elevated macrophage ROS, oxidative DNA damage, and increased susceptibility to inflammatory injury.

    Evidence PRDX3 knockout mouse; intratracheal LPS challenge; flow cytometry for ROS; 8-OHdG and protein carbonyl immunohistochemistry

    PMID:17316558

    Open questions at the time
    • Neurological phenotype in knockout not examined at this stage
    • Compensation by other peroxiredoxins not assessed
  7. 2016 Medium

    Discovery of a redox-dependent disulfide interaction between PRDX3 and UNG1 revealed that PRDX3 protects mitochondrial DNA repair capacity by shielding UNG1 from LonP1-mediated degradation under oxidative stress.

    Evidence Proteomics/MS identification of UNG1 as PRDX3 partner; Co-IP under oxidative stress; PRDX3 siRNA; LonP1 inhibition; mtDNA oxidation measurement

    PMID:27480846

    Open questions at the time
    • Stoichiometry and structural basis of disulfide linkage not defined
    • Whether interaction is constitutive or only stress-induced unclear
    • Single study without independent replication
  8. 2019 High

    Identification of SIRT3 as the specific deacetylase of PRDX3 at K253, with acetylation impairing dimerization and antioxidative activity, revealed the first defined post-translational regulatory switch controlling PRDX3 function.

    Evidence IP-based acetylation site mapping; SIRT3 knockdown and nicotinamide inhibition; K253R/Q mutagenesis; SIRT3 KO mouse ischemia-reperfusion model

    PMID:31655428

    Open questions at the time
    • Acetyltransferase responsible for K253 acetylation not identified
    • Whether other SIRT3 substrates mediate overlapping protection unclear
  9. 2021 High

    The discovery that biallelic PRDX3 loss-of-function mutations cause SCAR32 established PRDX3 as essential for cerebellar neuron survival, linking mitochondrial peroxide detoxification to neurodegeneration in humans.

    Evidence WGS/WES in patient families; patient fibroblast functional assays; PRDX3 knockdown in medulloblastoma cells; Drosophila pan-neuronal RNAi

    PMID:33889951

    Open questions at the time
    • Cerebellar selectivity of neurodegeneration not mechanistically explained
    • No mouse SCAR32 model generated
  10. 2022 Medium

    The PRDX3 D163E missense mutation was shown to cause protein aggregation and trigger both mitochondrial and ER unfolded protein responses, providing a gain-of-toxic-function mechanism for PRDX3-linked ataxia beyond simple loss of peroxidase activity.

    Evidence Heterologous expression in HeLa; CLEM ultrastructural analysis; aggregation assays; patient fibroblast analysis; primary cortical neuron morphology

    PMID:35766882

    Open questions at the time
    • Whether aggregation occurs in patient neurons in vivo not shown
    • Relative contributions of loss-of-function versus gain-of-toxic-function not dissected
  11. 2023 High

    The finding that lipid-peroxide-driven hyperoxidation of PRDX3 triggers its translocation from mitochondria to the plasma membrane, where it inhibits cystine uptake, established a non-canonical signaling function linking mitochondrial redox state to ferroptosis amplification.

    Evidence Ferroptosis induction; subcellular fractionation and immunofluorescence; cystine uptake assay; in vivo fatty liver disease models; hyperoxidized PRDX3-specific antibody

    PMID:37863053

    Open questions at the time
    • Mechanism of translocation from mitochondria to plasma membrane unknown
    • Direct molecular target at the plasma membrane mediating cystine uptake inhibition not identified
  12. 2024 High

    Resolution of dual submitochondrial targeting showed PRDX3 is sorted to both the matrix (via MPP/MIP) and the intermembrane space (via IMP), indicating distinct functional pools within mitochondria.

    Evidence Subfractionation of purified HEK293T mitochondria; alkaline carbonate extraction; in organello import; yeast IMP complex mutants

    PMID:39591905

    Open questions at the time
    • Functional role of IMS-localized PRDX3 not characterized
    • Relative stoichiometry of matrix versus IMS pools unknown
  13. 2024 Medium

    Multiple post-translational regulatory axes were mapped — TRIM39-mediated K48 ubiquitination at K73/K149, ERβ suppression of SUMOylation, PRDX3–PINK1 interaction supporting mitophagy, and YAP1–TEAD1 transcriptional activation — revealing the breadth of regulatory inputs converging on PRDX3 abundance and activity.

    Evidence Co-IP, site-directed mutagenesis, ubiquitination/SUMOylation assays, ChIP, xenograft and fibrosis models across multiple studies

    PMID:38097136 PMID:38195664 PMID:38945958 PMID:40912394

    Open questions at the time
    • Cross-talk between ubiquitination, SUMOylation, and acetylation on the same molecule not examined
    • Many interactions validated only by Co-IP in a single lab
  14. 2025 Medium

    Identification of SIRT4 as a second sirtuin deacetylating PRDX3 at K92 and KAT2A as the succinylase at K84 expanded the PTM code governing PRDX3, linking specific modifications to ferroptosis susceptibility and microglial polarization respectively.

    Evidence Co-IP, K92/K84 site mutagenesis, SIRT4 KO mice with liver I/R, KAT2A knockdown in TBI model with microglial polarization markers

    PMID:40457625 PMID:40765819

    Open questions at the time
    • Interplay between K92 deacetylation and K253 deacetylation not examined
    • Single-lab findings for each modification

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include the mechanism by which hyperoxidized PRDX3 translocates to the plasma membrane, the identity of the plasma membrane target through which it inhibits cystine uptake, the functional significance of the IMS-localized pool, high-resolution structural dynamics of the decamer under redox cycling, and how the multiple overlapping PTMs (acetylation, succinylation, SUMOylation, ubiquitination, palmitoylation) are integrated to tune PRDX3 activity in vivo.
  • Mechanism of mitochondria-to-plasma membrane translocation unknown
  • Direct cystine transporter target at plasma membrane not identified
  • Functional role of IMS pool not defined
  • No high-resolution structure of human PRDX3 decamer
  • Integrated PTM crosstalk model absent

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016209 antioxidant activity 4 GO:0016491 oxidoreductase activity 2
Localization
GO:0005739 mitochondrion 5 GO:0005886 plasma membrane 1
Pathway
R-HSA-8953897 Cellular responses to stimuli 5 R-HSA-392499 Metabolism of proteins 3 R-HSA-5357801 Programmed Cell Death 2 R-HSA-9612973 Autophagy 1
Partners
KAT2APINK1PPT1SIRT3SIRT4TNNI3KTRIM39UNG1

Evidence

Reading pass · 24 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 SP-22 (PRDX3) is a thioredoxin-dependent peroxide reductase in mitochondria; in the presence of mitochondrial thioredoxin (mt-Trx), NADPH, and a mitochondrial thioredoxin reductase, SP-22 catalyzes the reduction of H2O2 and tert-butyl hydroperoxide with concomitant NADPH oxidation, establishing the three-component electron relay: thioredoxin reductase → mt-Trx → SP-22 → peroxide. In vitro reconstitution with purified SP-22, mt-Trx, and partially purified NADPH-dependent Nbs2 reductase; oxyhemoglobin protection assay; NADPH oxidation measured spectrophotometrically in the presence of H2O2 or t-BuOOH European journal of biochemistry High 9363753
1995 SP-22 (PRDX3) functions as a radical scavenger in adrenocortical mitochondria, protecting radical-sensitive enzymes (tryptophan hydroxylase, glutamine synthetase, hemoglobin) from Fe²⁺/dithiothreitol-mediated oxidative damage; the protein was originally identified as a substrate of the mitochondrial ATP-dependent protease. In vitro radical-scavenging assay using Fe²⁺/dithiothreitol radical-generating system; enzyme activity protection assays with tryptophan hydroxylase and glutamine synthetase Biochemical and biophysical research communications Medium 7654218
2003 Bovine SP-22 (PRDX3) is a 2-Cys peroxiredoxin organized as a decameric toroid of five dimeric units; Cys-47 is the catalytic (peroxidatic) cysteine and forms an intermolecular disulfide with Cys-168 of the adjacent monomer; Cys-66 is not required for activity. The disulfide bonds are not required for the toroidal quaternary structure. Recombinant expression of wild-type and Cys→Ser mutants (C47S, C66S, C168S) in E. coli; circular dichroism; electron microscopy; peroxidase activity assays The Journal of biological chemistry High 12773537
2002 PRDX3 is a transcriptional target of c-Myc; Myc binds the PRDX3 genomic region surrounding exon 1 (chromatin immunoprecipitation); PRDX3 is required for Myc-mediated cell proliferation, neoplastic transformation, and apoptosis after glucose withdrawal; PRDX3 is essential for maintaining mitochondrial mass and membrane potential in transformed cells. Chromatin immunoprecipitation (ChIP) across the PRDX3 genomic locus; mycER induction system; PRDX3 knockdown/overexpression; mitochondria-specific fluorescent probes (JC-1, MitoTracker); transformation assays in rat and human cells Proceedings of the National Academy of Sciences of the United States of America High 12011429
1999 SP-22 (PRDX3) expression in bovine aortic endothelial cells is induced 1.5–4.6-fold by mitochondrial oxidative stresses (Fe²⁺/dithiothreitol, antimycin A, respiratory inhibitors); antisense depletion of SP-22 increases cellular lability to oxidative stress, and preconditioning with mild oxidative stress (which raises SP-22) confers tolerance to subsequent intense stress; SP-22 induction also occurs in vivo in rat myocardial infarction. Antisense oligodeoxynucleotide knockdown; oxidative stress tolerance assays; RT-PCR and western blot for mRNA and protein; in vivo rat myocardial infarction model The Journal of biological chemistry Medium 9890990
2007 PRDX3 (MER5) knockout mice show significantly elevated intracellular ROS in macrophages and increased susceptibility to LPS-induced lung inflammation, oxidative DNA damage (8-OHdG), and protein carbonylation, establishing PRDX3 as a required ROS scavenger in vivo. MER5/PRDX3 knockout mouse model; intratracheal LPS challenge; flow cytometry for intracellular ROS; 8-OHdG and protein carbonyl immunohistochemistry Biochemical and biophysical research communications High 17316558
2007 PRDX3 is localized to mitochondria in human lens epithelial cells and is specifically and selectively induced by H2O2 (as low as 2 µM) but not by tert-butyl hydroperoxide or heat shock, indicating a specific sensor/effector role for H2O2 in the lens. Immunofluorescence localization; RT-PCR and western blot; comparison of H2O2 vs. TBHP vs. heat-shock treatments in human lens epithelial cells and whole rat lenses Molecular vision Medium 17893648
1998 Human T cell cyclophilin18 (hCyP18) physically interacts with the thiol-specific antioxidant protein Aop1 (PRDX3 family member) and stimulates its enzymatic activity; the stimulation is specific to CyP18 and not observed with other PPIases. Yeast two-hybrid; co-immunoprecipitation; in vitro binding assay; enzymatic activity stimulation assay Journal of molecular biology Medium 9545370
2007 Antioxidant protein 1 (AOP-1/PRDX3) physically interacts with the cardiac-specific kinase TNNI3K via the ANK motif of TNNI3K, and co-expression of AOP-1 inhibits TNNI3K kinase activity in an in vitro kinase assay. Yeast two-hybrid screening; in vitro binding assay; co-immunoprecipitation in vivo; confocal immunofluorescence co-localization; in vitro kinase assay Biochemistry. Biokhimiia Medium 18205602
2019 SIRT3 directly deacetylates PRDX3 at lysine K253; acetylation of K253 impairs PRDX3 dimerization and antioxidative activity, while the deacetylation-mimicking K253R mutation enhances dimerization and mitochondrial protection. SIRT3 knockdown increases PRDX3 acetylation; inhibition of SIRTs by nicotinamide does not further increase PRDX3 acetylation in SIRT3-knockdown cells, confirming SIRT3 as the specific deacetylase. Immunoprecipitation to identify acetylation site; SIRT3 knockdown; nicotinamide inhibition; K253R and K253Q site-directed mutants; dimerization assay; mitochondrial ROS and apoptosis readouts; SIRT3 knockout mouse model of I/R injury Redox biology High 31655428
2020 High glucose conditions increase PRDX3 acetylation via SIRT1 degradation, which impairs SIRT3 activity toward PRDX3, leading to PRDX3 hyper-oxidation and mitochondrial dysfunction in pancreatic β-cells; SIRT3 physically interacts with PRDX3 as shown by co-immunoprecipitation, and activated SIRT3 prevents PRDX3 acetylation and hyper-oxidation. Co-immunoprecipitation (SIRT3-PRDX3 interaction); SIRT1 knockdown/inhibition (EX-527/siRNA); SIRT3 activity measurement; PRDX3 acetylation western blot; ROS and apoptosis assays in INS-1 and 1.1B4 cells Free radical biology & medicine Medium 32763411
2016 Under oxidative stress, PRDX3 binds to UNG1 (mitochondrial uracil-DNA glycosylase isoform 1) via a disulfide linkage, protecting UNG1 from ROS-mediated Lon protease 1 (LonP1)-dependent degradation and preventing mtDNA oxidation; PRDX3 knockdown aggravates UNG1 degradation. Proteomics/mass spectrometry to identify UNG1 binding partners; co-immunoprecipitation under oxidative stress; PRDX3 siRNA knockdown; LonP1 inhibition assay; mtDNA oxidation measurement Free radical biology & medicine Medium 27480846
2023 During ferroptosis, mitochondrial lipid peroxides trigger PRDX3 hyperoxidation (conversion of catalytic Cys to sulfinic/sulfonic acid); hyperoxidized PRDX3 translocates from mitochondria to the plasma membrane where it inhibits cystine uptake, amplifying ferroptosis. This identifies hyperoxidized PRDX3 as a specific ferroptosis marker and reveals a mechanism linking mitochondrial peroxide metabolism to cystine transport. In vitro ferroptosis induction; subcellular fractionation and immunofluorescence for PRDX3 translocation; cystine uptake assay; in vivo mouse models of alcoholic and non-alcoholic fatty liver disease; hyperoxidized PRDX3 antibody-based detection Molecular cell High 37863053
2022 PRDX3 mRNA is modified by m6A and the m6A reader YTHDF3 (but not YTHDF1 or YTHDF2) directly regulates PRDX3 translation in an m6A-dependent manner; PRDX3 suppresses hepatic stellate cell activation at least partially via the mitochondrial ROS/TGF-β1/Smad2/3 pathway. RNA pull-down/mass spectrometry to identify m6A readers interacting with PRDX3 mRNA; YTHDF1-3 individual knockdown; AAV9-mediated PRDX3 knockdown/overexpression in mice; ROS measurement; TGF-β1/Smad2/3 pathway analysis Redox biology Medium 35779442
2021 Biallelic loss-of-function mutations in PRDX3 cause autosomal recessive cerebellar ataxia (SCAR32); patient fibroblasts lacking PRDX3 show decreased glutathione peroxidase activity and decreased mitochondrial maximal respiratory capacity; PRDX3 knockdown in cerebellar medulloblastoma cells increases H2O2 and apoptosis susceptibility; pan-neuronal/glial Drosophila knockdown causes locomotor defects and reduced survival under oxidative stress. Whole-genome/exome sequencing; patient fibroblast functional assays (respiratory capacity, GPx activity); siRNA knockdown in DAOY cells; Drosophila pan-neuronal/glial RNAi model with locomotor and survival assays Brain : a journal of neurology High 33889951
2022 The PRDX3 p.D163E missense mutation causes protein instability, aggregate formation, and triggers unfolded protein responses via both mitochondrial and endoplasmic reticulum pathways; in HeLa cells expressing the mutation, mitochondria show severe membrane and cristae disorganization and lipid droplet accumulation; neurite morphology is altered in primary cortical neurons expressing the mutant. Whole exome sequencing; heterologous expression in HeLa cells; correlative light-electron microscopy (CLEM); mitochondrial functional parameters; biochemical aggregation assays; patient fibroblast analysis Human molecular genetics Medium 35766882
2023 ERβ suppresses PRDX3 SUMOylation, thereby reducing ROS accumulation and promoting osimertinib resistance in NSCLC; USP7 deubiquitinates and stabilizes ERβ, positioning the USP7–ERβ–PRDX3 SUMOylation axis as a resistance mechanism. Co-immunoprecipitation; SUMOylation assay; siRNA knockdown of ERβ and PRDX3; ROS measurement; in vitro and in vivo drug resistance assays Cancer letters Medium 38097136
2024 The E3 ubiquitin ligase TRIM39 directly interacts with PRDX3 and induces its proteasomal degradation via K48-linked ubiquitination at lysine residues K73 and K149, leading to ROS accumulation and inflammatory cytokine production that aggravates renal fibrosis. Co-immunoprecipitation (TRIM39-PRDX3 interaction); ubiquitination assay with K48-linkage specificity; site-directed mutagenesis of K73 and K149; TRIM39 knockdown in UUO mice and HK-2 cells; ROS and cytokine measurement Cell death discovery Medium 38195664
2024 Human PRDX3 undergoes dual submitochondrial localization: it is imported into the matrix via sequential cleavage by mitochondrial processing peptidase (MPP) and mitochondrial intermediate peptidase (MIP), and is also sorted to the intermembrane space (IMS) via the inner membrane peptidase (IMP) complex; both isoforms are soluble proteins. Subfractionation of highly purified HEK293T mitochondria; alkaline carbonate extraction; in organello import assays; heterologous yeast expression with IMP complex mutants; in silico presequence analysis Redox biology High 39591905
2025 SIRT4 physically interacts with PRDX3 and deacetylates it specifically at lysine K92; SIRT4 knockout exacerbates ferroptosis in liver ischemia-reperfusion injury, and this exacerbation is dependent on PRDX3 deacetylation at K92, as demonstrated by K92 mutant rescue experiments. Co-immunoprecipitation (SIRT4-PRDX3); site-directed mutagenesis (K92); SIRT4 knockout and liver-specific overexpression mouse models; ferroptosis inhibitor ferrostatin-1 rescue; liver-targeted LNP-mRNA delivery International journal of biological sciences Medium 40765819
2026 PPT1 is the depalmitoylase of PRDX3, catalyzing depalmitoylation at the catalytic cysteine C108; S-palmitoylation of C108 by PPT1 substrates inhibits PRDX3 antioxidant activity; genetic or chemical inhibition of PPT1 elevates PRDX3 S-palmitoylation, increases mitochondrial ROS, and induces cytotoxicity in multiple myeloma cells. Co-immunoprecipitation; palmitoylation assay (acyl-RAC); site-directed mutagenesis (C108); PPT1 genetic/chemical inhibition; xenograft tumor model; mitochondrial ROS measurement Cellular signalling Medium 41865945
2025 KAT2A (a succinyltransferase) interacts with PRDX3 and succinylates it at K84; KAT2A knockdown inhibits PRDX3 succinylation at K84, enhancing PRDX3 stability and promoting microglial M2 polarization over M1. K84 succinylation-mimetic mutation abolishes this polarization effect. Co-immunoprecipitation; succinylation immunoprecipitation; K84 site mutagenesis; KAT2A knockdown in LPS-activated BV2 cells and TBI mouse model; microglial polarization markers by qPCR Neurological research Medium 40457625
2024 PRDX3 interacts with PINK1 to stabilize Parkin-mediated mitophagy flux in nasopharyngeal carcinoma cells; PRDX3 knockdown disrupts PINK1/Parkin-dependent mitophagy, causing mitochondrial lipid peroxidation and apoptosis. Co-immunoprecipitation (PRDX3-PINK1); immunofluorescence co-localization; siRNA knockdown; mitophagy, ROS, and apoptosis assays; xenograft model Experimental cell research Medium 40912394
2024 YAP1 transcriptionally activates PRDX3 expression by directly interacting with TEAD1 at the PRDX3 promoter; forced Prdx3 expression inhibits alveolar epithelial cell senescence and mitochondrial dysfunction, and Prdx3 knockdown partially abrogates the anti-fibrotic effects of YAP1 overexpression. ChIP assay (YAP1-TEAD1 interaction at PRDX3 promoter); AAV-mediated overexpression/knockdown; cell senescence markers; mitochondrial function assays; bleomycin-induced fibrosis model in vivo Experimental & molecular medicine Medium 38945958

Source papers

Stage 0 corpus · 76 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2019 SIRT3-mediated deacetylation of PRDX3 alleviates mitochondrial oxidative damage and apoptosis induced by intestinal ischemia/reperfusion injury. Redox biology 191 31655428
2002 The c-Myc target gene PRDX3 is required for mitochondrial homeostasis and neoplastic transformation. Proceedings of the National Academy of Sciences of the United States of America 157 12011429
2007 Increased susceptibility of MER5 (peroxiredoxin III) knockout mice to LPS-induced oxidative stress. Biochemical and biophysical research communications 126 17316558
1997 SP-22 is a thioredoxin-dependent peroxide reductase in mitochondria. European journal of biochemistry 125 9363753
2023 Identification of hyperoxidized PRDX3 as a ferroptosis marker reveals ferroptotic damage in chronic liver diseases. Molecular cell 121 37863053
1999 Antioxidant function of the mitochondrial protein SP-22 in the cardiovascular system. The Journal of biological chemistry 114 9890990
1989 Cloning of a housekeeping-type gene (MER5) preferentially expressed in murine erythroleukemia cells. Gene 110 2583515
2010 Differential expression of peroxiredoxins in prostate cancer: consistent upregulation of PRDX3 and PRDX4. The Prostate 78 21031435
2013 MiR-383 is downregulated in medulloblastoma and targets peroxiredoxin 3 (PRDX3). Brain pathology (Zurich, Switzerland) 68 23227829
2022 The m6A reader YTHDF3-mediated PRDX3 translation alleviates liver fibrosis. Redox biology 67 35779442
1995 Possible function of SP-22, a substrate of mitochondrial ATP-dependent protease, as a radical scavenger. Biochemical and biophysical research communications 58 7654218
2003 Enhancement of mitochondrial oxidative stress and up-regulation of antioxidant protein peroxiredoxin III/SP-22 in the mitochondria of human pre-eclamptic placentae. Placenta 56 12828928
1998 Human T cell cyclophilin18 binds to thiol-specific antioxidant protein Aop1 and stimulates its activity. Journal of molecular biology 52 9545370
1994 Cloning and characterization of OSF-3, a new member of the MER5 family, expressed in mouse osteoblastic cells. Journal of biochemistry 45 8089076
1997 Copurification of vimentin, energy metabolism enzymes, and a MER5 homolog with nucleoside diphosphate kinase. Identification of tissue-specific interactions. The Journal of biological chemistry 44 9169432
1990 Antisense RNA of the latent period gene (MER5) inhibits the differentiation of murine erythroleukemia cells. Gene 44 2210385
2003 Structure-function analysis of recombinant substrate protein 22 kDa (SP-22). A mitochondrial 2-CYS peroxiredoxin organized as a decameric toroid. The Journal of biological chemistry 42 12773537
2012 Single nucleotide polymorphisms in the PRDX3 and RPS19 and risk of HPV persistence and cervical precancer/cancer. PloS one 38 22496757
2020 High glucose-induced PRDX3 acetylation contributes to glucotoxicity in pancreatic β-cells: Prevention by Teneligliptin. Free radical biology & medicine 37 32763411
2020 A novel circRNA, circNUP98, a potential biomarker, acted as an oncogene via the miR-567/ PRDX3 axis in renal cell carcinoma. Journal of cellular and molecular medicine 34 32729669
2021 Biallelic loss-of-function variations in PRDX3 cause cerebellar ataxia. Brain : a journal of neurology 31 33889951
2016 Silencing PRDX3 Inhibits Growth and Promotes Invasion and Extracellular Matrix Degradation in Hepatocellular Carcinoma Cells. Journal of proteome research 28 26983019
2023 USP7-mediated ERβ stabilization mitigates ROS accumulation and promotes osimertinib resistance by suppressing PRDX3 SUMOylation in non-small cell lung carcinoma. Cancer letters 26 38097136
2013 siRNA targeting of PRDX3 enhances cisplatin‑induced apoptosis in ovarian cancer cells through the suppression of the NF‑κB signaling pathway. Molecular medicine reports 25 23503975
2021 Carnosol alleviates nonalcoholic fatty liver disease by inhibiting mitochondrial dysfunction and apoptosis through targeting of PRDX3. Toxicology and applied pharmacology 24 34678374
2024 YAP1 inhibits the senescence of alveolar epithelial cells by targeting Prdx3 to alleviate pulmonary fibrosis. Experimental & molecular medicine 23 38945958
2016 Hydrogen peroxide mediated mitochondrial UNG1-PRDX3 interaction and UNG1 degradation. Free radical biology & medicine 21 27480846
2021 lnc-NLC1-C inhibits migration, invasion and autophagy of glioma cells by targeting miR-383 and regulating PRDX-3 expression. Oncology letters 18 34386062
2010 The combination of genetic variations in the PRDX3 gene and dietary fat intake contribute to obesity risk. Obesity (Silver Spring, Md.) 18 21127481
2020 AOP1, a New Live Cell Assay for the Direct and Quantitative Measure of Intracellular Antioxidant Effects. Antioxidants (Basel, Switzerland) 17 32492957
2014 Heart mitochondrial proteome study elucidates changes in cardiac energy metabolism and antioxidant PRDX3 in human dilated cardiomyopathy. PloS one 17 25397948
2007 Localization and H2O2-specific induction of PRDX3 in the eye lens. Molecular vision 16 17893648
2007 AOP-1 interacts with cardiac-specific protein kinase TNNI3K and down-regulates its kinase activity. Biochemistry. Biokhimiia 15 18205602
2022 Protein misfolding and clearance in the pathogenesis of a new infantile onset ataxia caused by mutations in PRDX3. Human molecular genetics 14 35766882
2021 Hsa_circ_0032131 knockdown inhibits osteoarthritis progression via the miR-502-5p/PRDX3 axis. Aging 14 34032607
2024 FXN targeting induces cell death in ovarian cancer stem-like cells through PRDX3-Mediated oxidative stress. iScience 13 39184439
2024 The E3 ubiquitin ligase TRIM39 modulates renal fibrosis induced by unilateral ureteral obstruction through regulating proteasomal degradation of PRDX3. Cell death discovery 11 38195664
2016 Plekhs1 and Prdx3 are candidate genes responsible for mild hyperglycemia associated with obesity in a new animal model of F344-fa-nidd6 rat. The Journal of veterinary medical science 11 27523322
2024 Activation of the FOXM1/ASF1B/PRDX3 axis confers hyperproliferative and antioxidative stress reactivity to gastric cancer. Cancer letters 10 38537775
2012 Identification of proteins containing redox-sensitive thiols after PRDX1, PRDX3 and GCLC silencing and/or glucose oxidase treatment in Hepa 1-6 cells. Journal of proteomics 10 22975676
2000 Expression of mitochondrial thioredoxin-dependent antioxidant protein, SP-22, in normal human and inflammatory mouse placentae. Placenta 10 11095935
1996 The cDNA sequence encoding bovine SP-22, a new defence system against reactive oxygen species in mitochondria. DNA sequence : the journal of DNA sequencing and mapping 10 8912927
2024 Human mitochondrial peroxiredoxin Prdx3 is dually localized in the intermembrane space and matrix subcompartments. Redox biology 9 39591905
2022 PRDX3 promotes resistance to cisplatin in gastric cancer cells. Journal of cancer research and therapeutics 9 36647961
2023 Knockdown of circSOD2 ameliorates osteoarthritis progression via the miR-224-5p/PRDX3 axis. Journal of orthopaedic surgery and research 8 37312219
2012 [MicroRNA383 regulates expression of PRDX3 in human medulloblastomas]. Zhonghua bing li xue za zhi = Chinese journal of pathology 8 23157748
2025 Histone lactylation protects against sevoflurane-induced cognitive impairment by regulating YTHDF3/PRDX3 mediated microglial pyroptosis in neonatal mice. International immunopharmacology 7 40022822
2022 Silencing of B7-H4 induces intracellular oxidative stress and inhibits cell viability of breast cancer cells via downregulating PRDX3. Neoplasma 7 35723197
2022 A novel biallelic variant further delineates PRDX3-related autosomal recessive cerebellar ataxia. Neurogenetics 7 36190665
2025 Empagliflozin attenuates renal damage in diabetic nephropathy by modulating mitochondrial quality control via Prdx3-PINK1 pathway. Biochemical pharmacology 6 39983849
2012 Involvement of Prx3, a Drosophila ortholog of the thiol-dependent peroxidase PRDX3, in age-dependent oxidative stress resistance. Biomedical research (Tokyo, Japan) 6 23124252
2024 Antioxidant PRDX3 gene therapy protects brain cells and prevents neurodegeneration in an animal model of Parkinson's disease. Neuropeptides 5 39736192
2022 A quantitative proteomic analysis reveals the potential roles of PRDX3 in neurite outgrowth in N2a-APPswe cells. Biochemical and biophysical research communications 5 35303681
2011 Immunohistochemical detection of metalloproteinase-9 (MMP-9), anti-oxidant like 1 protein (AOP-1) and synaptosomal-associated protein (SNAP-25) in the cerebella of dogs naturally infected with spontaneous canine distemper. Folia histochemica et cytobiologica 5 21526488
2024 Dexmedetomidine alleviates Hypoxia/reoxygenation-induced mitochondrial dysfunction in cardiomyocytes via activation of Sirt3/Prdx3 pathway. Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences 4 38407745
2023 Hyperoxidized PRDX3 as a specific ferroptosis marker. Life metabolism 4 38179338
2025 SIRT4-Mediated Deacetylation of PRDX3 Attenuates Liver Ischemia Reperfusion Injury by Suppressing Ferroptosis. International journal of biological sciences 3 40765819
2021 Confirmation of PRDX3 c.568G>C as the Genetic Basis of Punctiform and Polychromatic Pre-Descemet Corneal Dystrophy. Cornea 3 34369396
2025 The miR-320a/PRDX3 Axis Alleviates the Oxidative Stress and Fibrotic Alterations in Fibroblasts in Thyroid Eye Disease. Investigative ophthalmology & visual science 2 40657967
2025 PRDX5 Regulates Mitochondrial Function and Nuclear Spreading in Myogenesis and Acts With PRDX3 to Delay Muscle Aging. Journal of cachexia, sarcopenia and muscle 2 41147088
2024 Ablation of Shank1 Protects against 6-OHDA-induced Cytotoxicity via PRDX3-mediated Inhibition of ER Stress in SN4741 Cells. CNS & neurological disorders drug targets 2 36797610
2014 Comparative study of Hsp27, GSK3β, Wnt1 and PRDX3 in Hirschsprung's disease. International journal of experimental pathology 2 24773279
2000 [Cell division in the volume of Flavobacterium sp.22 colonies]. Mikrobiologiia 2 10776626
2025 KAT2A knockdown induces microglia M2 polarization by succinylation of PRDX3 after traumatic brain injury. Neurological research 1 40457625
2025 Methylation of PRDX3 Expression Alleviate Ferroptosis and Oxidative Stress in Patients with Osteoarthritis Cartilage Injury. Archives of rheumatology 1 40757971
2025 The pathogenesis of benign prostatic hyperplasia and the roles of Prdx3, oxidative stress, pyroptosis and autophagy:a review. Frontiers in oncology 1 40837016
2025 Lycorine ameliorates astrocytic apoptosis and inflammation in cerebral ischemia/reperfusion injury via inhibiting mitochondrial dysfunction via SIRT1-mediated SIRT3/PRDX3 activation. Pathology, research and practice 1 40975001
2025 Atractylodin inhibits ferroptosis in sepsis‑induced acute gastrointestinal injury via SIRT3/PRDX3. Molecular medicine reports 1 40999974
2019 Chicken PRDX3 is required for proliferation of chicken embryo fibroblast cells. British poultry science 1 31615265
2026 Membrane-translocated SO₂/₃-PRDX3 disrupts cystine uptake and GPX4 activity: A pivotal mechanism of boron-induced renal ferroptosis in broiler. Ecotoxicology and environmental safety 0 41775182
2026 PPT1 regulates mitochondrial redox by depalmitoylating PRDX3. Cellular signalling 0 41865945
2025 PRDX3 promotes nasopharyngeal carcinoma tumor growth by regulating PINK1/Parkin pathway-dependent lipid peroxidation and mitochondrial dysfunction. Experimental cell research 0 40912394
2025 PRDX3 Promotes Lymph Node Metastasis in Cervical Cancer by Activating NF-κB Signaling Pathway and Anoikis Resistance. International journal of medical sciences 0 41049446
2025 Trace component fishing strategy based on offline two-dimensional liquid chromatography combined with PRDX3-surface plasmon resonance for Uncaria alkaloids. Journal of pharmaceutical analysis 0 41050113
2025 A homozygous PRDX3 pathogenic variant in a paediatric case of spinocerebellar ataxia type 32. Neurogenetics 0 41351775
2019 Molecular cloning, expression, purification, and functional characterization of SP-22 gene from Bombyx mori. Journal of cellular biochemistry 0 31099441