- Length
- 284 aa
- Mass
- 30.4 kDa
- Annotated
- 2026-04-29
40 papers in source corpus
2 papers cited in narrative
6 extracted findings
Mechanistic narrative
Synthesis pass · prose summary of the discoveries below
Insufficient on-target evidence to synthesize a narrative — discovery timeline does not match the canonical PEF1 protein.
Mechanism profile
Synthesis pass · controlled-vocabulary classification · explore literature graph →
No controlled-vocabulary terms were assigned to this entry.
Evidence
Reading pass · 6 per-paper findings extracted from the source corpus
| Year | Finding | Method | Journal | Conf | PMIDs |
|---|---|---|---|---|---|
| 2019 | The penta-EF-hand protein PEF1 of Neurospora crassa accumulates at sites of plasma membrane injury in a Ca2+-dependent manner, as shown by live-cell fluorescence imaging of GFP-tagged PEF1. Site-directed mutagenesis of Ca2+-binding EF-hand domains abolished both the spatial recruitment dynamics and the membrane repair function, establishing that Ca2+ binding is essential for PEF1 activity at membrane damage sites. | Live-cell fluorescence/GFP imaging, site-directed mutagenesis of Ca2+-binding domains, gene deletion in wild-type and lysis-prone mutant backgrounds | Genetics | Medium | 31270133 |
| 2019 | Deletion of pef1 in N. crassa sensitizes cells to membrane disruption during cell-cell fusion and in the presence of pore-forming drugs (e.g., the plant defense compound tomatine), demonstrating that PEF1 is required for plasma membrane integrity and repair in fungi. | Gene knockout (pef1 deletion) in wild-type and lysis-prone mutant backgrounds; treatment with pore-forming drug tomatine; cell fusion assays | Genetics | Medium | 31270133 |
| 2019 | PEF1-mediated membrane repair in N. crassa is associated with compartmentation of the syncytial fungal colony in response to antifungal treatment, as revealed by subcellular localization studies with GFP-PEF1. | GFP tagging and fluorescence imaging during antifungal drug treatment | Genetics | Low | 31270133 |
| 2026 | In Candida albicans, Pef1 (penta-EF-hand ortholog of human ALG-2/PEF1) localizes to sites of polarized growth in yeast and hyphal cells, and redistributes to punctate spots at the plasma membrane upon treatment with amphotericin B or tomatine, indicating Ca2+-dependent recruitment to sites of membrane perturbation. | GFP-tagging and fluorescence microscopy; treatment with polyene drug amphotericin B and saponin tomatine; genetic deletion of calcineurin A (cna1) and its transcription factor crz1 | G3 (Bethesda, Md.) | Medium | 41920808 |
| 2026 | Deletion of PEF1 in C. albicans results in failure of hyphae to maintain plasma membrane integrity in serum, and the pef1Δ mutant shows attenuated virulence in an insect larvae infection model, establishing a role for Pef1 in membrane integrity during pathogenic growth. | Gene deletion (pef1Δ), plasma membrane integrity assays in serum, insect larvae infection model (in vivo virulence assay) | G3 (Bethesda, Md.) | Medium | 41920808 |
| 2026 | Loss of calcineurin A (Cna1), but not its downstream transcription factor Crz1, phenocopies the punctate membrane localization of GFP-Pef1 in C. albicans, placing Pef1 downstream of calcineurin in the membrane stress response pathway. | Genetic deletion of cna1 and crz1; GFP-Pef1 localization imaging | G3 (Bethesda, Md.) | Low | 41920808 |
Source papers
Stage 0 corpus · 40 papers · ranked by NIH iCite citations
| Year | Title | Journal | Citations | PMID |
|---|---|---|---|---|
| 2005 | Towards a proteome-scale map of the human protein-protein interaction network. | Nature | 2090 | 16189514 |
| 2002 | Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. | Proceedings of the National Academy of Sciences of the United States of America | 1479 | 12477932 |
| 2015 | The BioPlex Network: A Systematic Exploration of the Human Interactome. | Cell | 1118 | 26186194 |
| 2017 | Architecture of the human interactome defines protein communities and disease networks. | Nature | 1085 | 28514442 |
| 2014 | A proteome-scale map of the human interactome network. | Cell | 977 | 25416956 |
| 2012 | The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts. | Molecular cell | 973 | 22681889 |
| 2020 | A reference map of the human binary protein interactome. | Nature | 849 | 32296183 |
| 2003 | Complete sequencing and characterization of 21,243 full-length human cDNAs. | Nature genetics | 754 | 14702039 |
| 2021 | Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. | Cell | 705 | 33961781 |
| 2012 | A census of human soluble protein complexes. | Cell | 689 | 22939629 |
| 2011 | Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. | Briefings in bioinformatics | 656 | 21873635 |
| 2008 | An empirical framework for binary interactome mapping. | Nature methods | 652 | 19060904 |
| 2008 | Large-scale proteomics and phosphoproteomics of urinary exosomes. | Journal of the American Society of Nephrology : JASN | 607 | 19056867 |
| 2015 | A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface. | Cell | 433 | 26638075 |
| 2022 | OpenCell: Endogenous tagging for the cartography of human cellular organization. | Science (New York, N.Y.) | 432 | 35271311 |
| 2005 | Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. | Genome research | 409 | 16344560 |
| 2015 | Panorama of ancient metazoan macromolecular complexes. | Nature | 407 | 26344197 |
| 2004 | 14-3-3-affinity purification of over 200 human phosphoproteins reveals new links to regulation of cellular metabolism, proliferation and trafficking. | The Biochemical journal | 372 | 14744259 |
| 2012 | Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins. | Nature | 319 | 22810586 |
| 2010 | Dynamics of cullin-RING ubiquitin ligase network revealed by systematic quantitative proteomics. | Cell | 318 | 21145461 |
| 2006 | Disrupted function and axonal distribution of mutant tyrosyl-tRNA synthetase in dominant intermediate Charcot-Marie-Tooth neuropathy. | Nature genetics | 317 | 16429158 |
| 2019 | Intrinsically Disordered Protein TEX264 Mediates ER-phagy. | Molecular cell | 296 | 31006538 |
| 2003 | The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. | Genome research | 285 | 12975309 |
| 2007 | hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. | Genomics | 222 | 17207965 |
| 2010 | MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis. | Immunology and cell biology | 221 | 20458337 |
| 2011 | Toward an understanding of the protein interaction network of the human liver. | Molecular systems biology | 207 | 21988832 |
| 2011 | Next-generation sequencing to generate interactome datasets. | Nature methods | 200 | 21516116 |
| 2005 | Large-scale analysis of the human ubiquitin-related proteome. | Proteomics | 154 | 16196087 |
| 2013 | In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine. | Proteomics | 138 | 23533145 |
| 2018 | Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains. | Molecular cell | 136 | 30554943 |
| 1995 | The enhancer in the long control region of human papillomavirus type 16 is up-regulated by PEF-1 and down-regulated by Oct-1. | Journal of virology | 38 | 7769658 |
| 2020 | The S. pombe CDK5 ortholog Pef1 regulates sexual differentiation through control of the TORC1 pathway and autophagy. | Journal of cell science | 11 | 32788233 |
| 2006 | Molecular analysis of the 21-kb bacteriocin-encoding plasmid pEF1 from Enterococcus faecium 6T1a. | Plasmid | 10 | 16893567 |
| 2019 | Plasma Membrane Integrity During Cell-Cell Fusion and in Response to Pore-Forming Drugs Is Promoted by the Penta-EF-Hand Protein PEF1 in Neurospora crassa. | Genetics | 6 | 31270133 |
| 2024 | Characterization of Two Novel Endolysins from Bacteriophage PEF1 and Evaluation of Their Combined Effects on the Control of Enterococcus faecalis Planktonic and Biofilm Cells. | Antibiotics (Basel, Switzerland) | 4 | 39335057 |
| 2021 | The S. pombe CDK5 Orthologue Pef1 Cooperates with Three Cyclins, Clg1, Pas1 and Psl1, to Promote Pre-Meiotic DNA Replication. | Biomolecules | 3 | 33445784 |
| 2020 | The CDK Pef1 and protein phosphatase 4 oppose each other for regulating cohesin binding to fission yeast chromosomes. | eLife | 2 | 31895039 |
| 1998 | PEF-1, an epithelial cell transcription factor which activates the long control region of human papillomavirus type 16, is glycosylated with N-acetylglucosamine. | The Journal of general virology | 2 | 9820151 |
| 2026 | The penta-EF-hand protein Pef1 of Candida albicans functions at sites of membrane perturbation to support polarized growth and membrane integrity. | G3 (Bethesda, Md.) | 0 | 41920808 |
| 2024 | In Vivo Proximity Labeling Identifies a New Function for the Lifespan and Autophagy-regulating Kinase Pef1, an Ortholog of Human Cdk5. | bioRxiv : the preprint server for biology | 0 | 38915521 |