Affinage

PCYT1A

Choline-phosphate cytidylyltransferase A · UniProt P49585

Length
367 aa
Mass
41.7 kDa
Annotated
2026-06-10
100 papers in source corpus 16 papers cited in narrative 16 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 5/5 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PCYT1A encodes CCTα, the rate-limiting enzyme of the CDP-choline pathway that converts phosphocholine to CDP-choline for phosphatidylcholine (PC) biosynthesis, and is essential for early development—complete loss in mice prevents blastocyst formation and causes embryonic lethality by E3.5 (PMID:15798219). The enzyme is amphitropic: it resides in the nucleoplasm under basal conditions and translocates to the inner nuclear membrane in response to membrane phospholipid packing defects (stored curvature elastic stress), a mechanism conserved across yeast, fly, and mammalian cells, with its α-helical M-domain mediating lipid-dependent membrane binding (PMID:29754800, PMID:32461215). This membrane-sensing activity couples CCTα to membrane biogenesis programs, including expansion of the nucleoplasmic reticulum together with nuclear lamins (PMID:21504799), recruitment to PML-dependent nuclear lipid droplets via the M-domain (PMID:32461215), and XBP-1-driven ER membrane expansion under lipid stress (PMID:26174230). Its localization and activity are tightly regulated: Ca2+-triggered binding of 14-3-3ζ to a C-terminal phosphoserine motif drives nuclear import and sustains PC synthesis and cell viability during infection (PMID:20007511), an autoinhibitory helix suppresses constitutive lipid-independent activity (PMID:30559292), and the enzyme acts as a negative regulator of mTORC1 signaling (PMID:32703435). In disease, homozygous loss-of-function mutations cause spondylometaphyseal dysplasia with cone-rod dystrophy and, depending on the specific allele, isolated retinal dystrophy (PMID:24387991, PMID:28272537); catalytic-domain missense mutations act by destabilizing folding and impairing kinetics, while an autoinhibitory-helix deletion produces a constitutively hyperactive enzyme (PMID:30559292).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1993 Medium

    Establishing the chromosomal location of the murine gene provided the genomic foundation for subsequent structural and functional dissection of CCTα.

    Evidence interspecific backcross Southern blot mapping and cDNA cloning in mouse

    PMID:8307580

    Open questions at the time
    • No protein function or regulation addressed
    • Human locus not mapped here
  2. 1997 High

    Resolving the gene's exon-intron architecture revealed that functional protein modules map cleanly to distinct exons, framing CCTα as a multi-domain enzyme with separable localization, catalytic, membrane-binding, and phosphorylation functions.

    Evidence genomic cloning, 5'-RACE, exon-intron mapping and promoter analysis of murine Ctpct

    PMID:9148929

    Open questions at the time
    • Domain functions inferred from exon boundaries, not yet tested biochemically
    • Promoter element function not yet validated
  3. 1999 High

    Identifying Sp1/AP1-dependent promoter elements answered how PCYT1A transcription is driven, linking enzyme abundance to defined regulatory regions.

    Evidence promoter deletion-luciferase reporters, DNase I footprinting, EMSA, and Sp1-deficient Drosophila cell rescue

    PMID:10216289

    Open questions at the time
    • Physiological signals controlling these factors not defined
    • An unidentified transcription factor remains uncharacterized
  4. 2005 High

    Genetic knockout settled whether CCTα is dispensable, showing it is absolutely required for early embryogenesis and that the β isoform does not compensate in adult tissues.

    Evidence Cre-lox deletion in mice with enzymatic, radiolabeling, and phospholipid mass readouts

    PMID:15798219

    Open questions at the time
    • Tissue-specific requirements beyond lung not dissected
    • Mechanism of lethality (which PC-dependent process fails first) not resolved
  5. 2005 Medium

    Lipid-mediator experiments connected CCTα activity to cell-fate decisions, showing biphasic activation then caspase cleavage/nuclear export drives apoptosis.

    Evidence PtdCho synthesis assays, caspase inhibitors, CCTα overexpression and DAG measurements in MT58 cells

    PMID:16097951

    Open questions at the time
    • Caspase cleavage site and in vivo relevance not established
    • Link between PC synthesis and apoptosis execution remains correlative
  6. 2009 High

    Identifying Ca2+-triggered 14-3-3ζ binding to a C-terminal phosphoserine motif answered how CCTα nuclear import is signal-regulated and tied it to survival during bacterial infection.

    Evidence Co-IP, siRNA, overexpression, nuclear fractionation and infection-model viability assays in lung epithelia

    PMID:20007511

    Open questions at the time
    • Kinase generating the phosphoserine motif not identified here
    • Generality beyond lung epithelia untested
  7. 2011 Medium

    Testing the role of nuclear architecture clarified that CCTα drives intranuclear membrane (nucleoplasmic reticulum) expansion via membrane deformation, while bulk nuclear envelope structure has minimal impact on its activity.

    Evidence lamin RNAi, progerin expression, CCTα imaging and choline radiolabeling in fibroblasts

    PMID:21504799

    Open questions at the time
    • Several negative results (lamin depletion did not change PC synthesis)
    • HGPS PC deficit attributed to choline transport, not CCTα itself
  8. 2014 Medium

    Human genetics established PCYT1A as a disease gene, linking loss-of-function mutations causally to spondylometaphyseal dysplasia with cone-rod dystrophy and demonstrating PC synthesis is essential for skeletal and retinal development.

    Evidence whole-exome sequencing and phenotyping of two Brazilian families

    PMID:24387991

    Open questions at the time
    • Tissue-specific basis for skeletal/retinal vulnerability unexplained
    • Genotype-phenotype mechanism not yet biochemically dissected
  9. 2015 Medium

    Linking XBP-1 to CCTα upregulation answered how lipid-stress signaling expands the ER, positioning CCTα as an effector of membrane biogenesis under saturated-fat load.

    Evidence siRNA of XBP-1/CCTα, ER stress inhibitors, TEM and phospholipid staining in macrophages

    PMID:26174230

    Open questions at the time
    • Direct transcriptional control of PCYT1A by XBP-1 not proven
    • In vivo significance not addressed
  10. 2017 Medium

    Identifying patients with isolated retinal dystrophy expanded the PCYT1A phenotypic spectrum, showing mutation-dependent variability in which tissues are affected.

    Evidence next-generation sequencing with skeletal and metabolic exclusion in two Italian families

    PMID:28272537

    Open questions at the time
    • Molecular basis for phenotypic restriction to retina unknown
    • Small patient number
  11. 2018 High

    Combining structural and biochemical analysis explained disease mechanism at the protein level: catalytic-domain mutations destabilize folding/kinetics while an autoinhibitory-helix deletion causes constitutive hyperactivation.

    Evidence expression of WT/mutant CCTs, purification, DSF Tm measurements, kinetics and anionic-vesicle activation assays

    PMID:30559292

    Open questions at the time
    • No full-length structure of the activated enzyme
    • Cellular consequences of hyperactive E280del not tested in vivo
  12. 2020 Medium

    Two studies extended CCTα beyond canonical metabolism: it is recruited to PML-dependent nuclear lipid droplets via its M-domain and acts as a negative regulator of mTORC1 in lung cancer cells.

    Evidence PML knockout imaging and lipid mass assays; siRNA knockdown with rapamycin/RAPTOR epistasis in adenocarcinoma cells

    PMID:32461215 PMID:32703435

    Open questions at the time
    • Molecular link between CCTα/PC and mTORC1 not defined
    • Whether nLD recruitment and mTORC1 regulation are connected unknown
  13. 2024 Medium

    Defining an AKT2/CCTα phosphorylation axis explained how stromal PC production is signal-controlled and drives drug resistance, identifying Ser315/319/323 as AKT2 targets that boost secreted PC and JAK2/STAT3 signaling.

    Evidence phosphoproteomics, lipidomics, AKT2/CCTα co-IP, Ca2+ manipulation and FAK-inhibitor treatment in cancer-associated fibroblasts

    PMID:38280862

    Open questions at the time
    • Direct demonstration that phosphorylation activates catalysis not isolated from localization
    • Generality beyond esophageal squamous carcinoma untested

Open questions

Synthesis pass · forward-looking unresolved questions
  • How specific PCYT1A alleles produce tissue-restricted disease (skeletal vs retinal vs lipodystrophy) and how CCTα activity is mechanistically coupled to mTORC1 remain unresolved.
  • No molecular explanation for genotype-tissue specificity
  • Biochemical link from PC synthesis to mTORC1 suppression undefined
  • No high-resolution structure of the membrane-activated full-length enzyme

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016740 transferase activity 4 GO:0008289 lipid binding 3
Localization
GO:0005635 nuclear envelope 2 GO:0005654 nucleoplasm 2 GO:0005783 endoplasmic reticulum 1 GO:0005811 lipid droplet 1
Pathway
GO:0016740 transferase activity 1
Partners
AKT2PMLYWHAZ

Evidence

Reading pass · 16 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2018 PCYT1A (CCTα), the rate-limiting enzyme of PC synthesis, is intranuclear under basal conditions and re-locates to the inner nuclear membrane in response to membrane phospholipid packing defects (stored curvature elastic stress), a mechanism conserved in yeast, fly, and mammalian cells. Nuclear localization signal mutants can compensate for loss of endogenous PCYT1A in yeast and fly photoreceptors. Live-cell imaging, lipidomic analysis, data-driven modeling, nuclear localization signal mutagenesis, genetic rescue experiments in yeast and Drosophila Developmental Cell High 29754800
2005 CCTα (encoded by Pcyt1a) catalyzes the rate-controlling step in CDP-choline pathway PC biosynthesis; Pcyt1a-/- knockout mice fail to form blastocysts and die by embryonic day 3.5, demonstrating that CCTα is essential for early embryonic development. Heterozygous mice show reduced lung PtdCho mass but no change in liver PtdCho, and CCTbeta is not upregulated to compensate in adult tissues. Cre-lox conditional gene deletion, in situ hybridization, enzymatic activity assays, real-time PCR, radiolabeled choline incorporation into isolated hepatocytes, phospholipid mass measurements Molecular and Cellular Biology High 15798219
1997 The murine PCYT1A (Ctpct) gene spans ~26 kb and comprises 9 exons whose boundaries align with distinct functional protein domains: exon 2 encodes the nuclear localization signal, exons 4–7 the catalytic domain, exon 8 the α-helical membrane-binding domain, and exon 9 the C-terminal phosphorylation domain. Two transcriptional start sites were identified 35 nt apart; the promoter lacks TATA/CAAT boxes and contains GC-rich regions with Sp1, AP1, AP2, AP3, Y1, and TFIIIA binding sites in two clusters. Genomic cloning and sequencing, 5'-RACE PCR, exon-intron mapping, promoter analysis Journal of Biological Chemistry High 9148929
1999 Transcription of the murine PCYT1A (Ctpct) gene is regulated by Sp1, AP1, and an unknown transcription factor binding to positive regulatory elements between -130 and -52 bp and basal elements between -52 and +38 bp upstream of the transcription start site. Promoter deletion constructs linked to luciferase reporter, transient transfection, DNase I protection assays, electromobility-shift assays (EMSA), co-transfection in Sp1-deficient Drosophila cells Biochimica et Biophysica Acta High 10216289
2009 CCTα nuclear import in lung epithelia is triggered by extracellular Ca2+ and requires binding to 14-3-3ζ. Helix G of 14-3-3ζ interacts with a phosphoserine motif in the CCTα C-terminal domain (residues 328–343). 14-3-3ζ is both sufficient and required for CCTα nuclear import and for maintaining phosphatidylcholine synthesis and cell viability during Pseudomonas aeruginosa infection. Co-immunoprecipitation, siRNA knockdown, overexpression, nuclear fractionation, Ca2+ manipulation, cell viability assays, infection model FASEB Journal High 20007511
2011 CCTα and nuclear lamins cooperate to form an intranuclear membrane network (nucleoplasmic reticulum, NR); lipid activation of CCTα drives its translocation to the nuclear envelope and NR expansion by membrane deformation. However, depletion of lamin A/C or lamin B1 alone had no effect on PC synthesis, and overall nuclear envelope structure has minimal impact on CCT activity and PC synthesis. In HGPS fibroblasts, CCTα mislocalized to the cytoplasm/nuclear envelope with a 2-fold reduction in PC synthesis attributable to reduced choline transporter activity rather than CCTα activity itself. RNAi silencing of lamins, expression of progerin (HGPS mutant lamin A), CCTα localization imaging, choline radiolabeling, subcellular fractionation Biochimica et Biophysica Acta Medium 21504799
2005 Farnesol (FOH) and oleyl alcohol first transiently activate CCTα and PtdCho synthesis (accompanied by DAG depletion), then promote caspase-mediated cleavage and nuclear export of CCTα leading to PtdCho synthesis inhibition and apoptosis. Stable overexpression of CCTα confers partial resistance to FOH-induced apoptosis; restoration of PtdCho synthesis by oleate or DiC8 also protects. Radioactive PtdCho synthesis assays, caspase inhibitors, CCTα overexpression in MT58 cells, DAG mass measurements, nuclear export imaging Biochemical Journal Medium 16097951
2015 Palmitate-induced cisternal ER expansion in RAW 264.7 macrophages depends on XBP-1-driven upregulation of CCTα mRNA expression, leading to increased phospholipid synthesis and ER membrane expansion. Downregulation of XBP-1 or CCTα attenuates palmitate-induced phospholipid accumulation and ER expansion. siRNA knockdown of XBP-1 and CCTα, ER stress inhibitors (4-PBA, TUDCA), transmission electron microscopy, RT-PCR, phospholipid staining Lipids in Health and Disease Medium 26174230
2020 Nuclear lipid droplets (nLDs) form on the inner nuclear membrane via PML-containing lipid-associated PML structures (LAPS). CCTα is recruited to nLDs via its α-helical M-domain (not correlated with its activator DAG). PML knockout reduces nLDs with associated CCTα by 40–50%, inhibits Lipin1 and DAG recruitment, and reduces phosphatidylcholine and triacylglycerol synthesis. PML knockout cells, high-resolution immunofluorescence imaging, lipid mass measurements, domain mutant overexpression Life Science Alliance Medium 32461215
2014 Homozygous loss-of-function mutations in PCYT1A, encoding the enzyme that converts phosphocholine to CDP-choline in the phosphatidylcholine biosynthesis pathway, cause spondylometaphyseal dysplasia with cone-rod dystrophy (SMD-CRD) in humans, establishing PCYT1A enzymatic activity as essential for normal skeletal and retinal development. Whole-exome sequencing, clinical phenotyping of affected individuals from two Brazilian families, identification of two homozygous PCYT1A mutations American Journal of Human Genetics Medium 24387991
2017 Mutations in PCYT1A can cause isolated retinal dystrophy without skeletal or metabolic involvement, expanding the phenotypic spectrum of PCYT1A loss-of-function beyond SMD-CRD and lipodystrophy. Next-generation sequencing, clinical evaluation (whole-skeleton X-ray, metabolic assessment, liver ultrasound) of three patients from two Italian families European Journal of Human Genetics Medium 28272537
2018 Disease-linked missense mutations in the PCYT1A catalytic domain (e.g., V142M, P150A) produce aggregated, non-refolding enzymes; other catalytic domain mutations destabilize folding (lower unfolding transition temperatures) and impair substrate Km values. A linker mutation (R223S) impairs enzyme kinetics. A single-amino-acid deletion in the autoinhibitory helix (E280del) increases constitutive (lipid-independent) activity ~4-fold and enhances the response to anionic lipid vesicles ~4-fold. Expression of WT and mutant CCTs in COS-1 cells, protein purification, denaturation/refolding assays, differential scanning fluorimetry (Tm measurements), enzyme kinetics, lipid activation assays with anionic vesicles Journal of Biological Chemistry High 30559292
2020 PCYT1A knockdown in lung adenocarcinoma cells enhances cell proliferation and migration by activating mTORC1 signaling; these effects are abolished by rapamycin treatment or RAPTOR depletion, placing PCYT1A upstream as a negative regulator of mTORC1. siRNA knockdown, rapamycin treatment, RAPTOR depletion, cell proliferation and migration assays, Western blotting for mTORC1 pathway components Biochemical and Biophysical Research Communications Medium 32703435
1993 The murine CTP:phosphocholine cytidylyltransferase gene (Ctpct/PCYT1A) is located on mouse chromosome 16 between the Smst and Stf-1 genes. Southern blot hybridization of interspecific backcross progeny DNA, genomic cloning and sequencing of murine cDNA Genomics Medium 8307580
2024 In cancer-associated fibroblasts (CAFs), FAK inhibitor treatment elevates intracellular Ca2+, promoting formation of an AKT2/CCTα complex, leading to phosphorylation of CCTα at Ser315/319/323 by AKT2, which enhances stromal phosphatidylcholine (PC) production; secreted PCs then activate the JAK2/STAT3 pathway in tumor cells, mediating FAK inhibitor resistance in esophageal squamous cell carcinoma. Phosphoproteomics, untargeted metabolomics/lipidomics, co-immunoprecipitation of AKT2/CCTα complex, Ca2+ manipulation, FAK inhibitor treatment, JAK2/STAT3 pathway analysis, clinical sample validation Signal Transduction and Targeted Therapy Medium 38280862
2006 A SNP in PCYT1A (rs939883 genotype AA) is associated with approximately 2-fold increased risk of spina bifida in a California population, suggesting that PCYT1A function in choline/phosphatidylcholine metabolism may influence neural tube closure. Fluorescence-based allelic discrimination (SNP genotyping) in 103 spina bifida cases and 338 controls BMC Medicine Low 17184542

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2009 Cancer/testis (CT) antigens: potential targets for immunotherapy. Cancer science 455 19719775
2013 Nano-sized CT contrast agents. Advanced materials (Deerfield Beach, Fla.) 398 23553799
2008 SPECT/CT. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 201 18632825
2001 Intracranial hemorrhage: principles of CT and MRI interpretation. European radiology 169 11511901
2000 CT evaluation of renovascular disease. Radiographics : a review publication of the Radiological Society of North America, Inc 139 10992021
2010 Exendin-4-based radiopharmaceuticals for glucagonlike peptide-1 receptor PET/CT and SPECT/CT. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 137 20595511
1993 CT evaluation of amyloidosis: spectrum of disease. Radiographics : a review publication of the Radiological Society of North America, Inc 126 8290725
2003 Expression of cancer/testis (CT) antigens in lung cancer. Lung cancer (Amsterdam, Netherlands) 116 14512184
2018 PCYT1A Regulates Phosphatidylcholine Homeostasis from the Inner Nuclear Membrane in Response to Membrane Stored Curvature Elastic Stress. Developmental cell 115 29754800
2000 Renal lymphoma: CT patterns with emphasis on helical CT. Radiographics : a review publication of the Radiological Society of North America, Inc 101 10682781
2009 CT-X antigen expression in human breast cancer. Proceedings of the National Academy of Sciences of the United States of America 96 19651608
2005 Early embryonic lethality in mice with targeted deletion of the CTP:phosphocholine cytidylyltransferase alpha gene (Pcyt1a). Molecular and cellular biology 95 15798219
2000 Lymph node metastases: CT and MRI. European journal of radiology 90 10699739
1996 CT and MR imaging of abdominal liposarcoma. AJR. American journal of roentgenology 88 8610559
2011 CT findings of pulmonary nocardiosis. AJR. American journal of roentgenology 79 21785052
2011 Cancer/testis (CT) antigens, carcinogenesis and spermatogenesis. Spermatogenesis 75 22319669
1995 Uncommon pneumoconioses: CT and pathologic findings. Radiology 74 7480684
2001 CT signs and patterns of lung disease. Radiologic clinics of North America 73 11699664
2012 CT urography for hematuria. Nature reviews. Urology 69 22410682
1999 CT of epithelial ovarian tumors. Radiographics : a review publication of the Radiological Society of North America, Inc 68 10517447
2005 Expression of cancer/testis (CT) antigens in Chinese hepatocellular carcinoma and its correlation with clinical parameters. Cancer letters 67 15723723
2002 Subsecond multisection CT of regional pulmonary ventilation. Academic radiology 67 11918366
2012 Renal cell carcinoma: attenuation values on unenhanced CT. AJR. American journal of roentgenology 65 22528901
2007 CT and MR characteristics of cerebral sparganosis. AJNR. American journal of neuroradiology 65 17885230
2019 Ultrasound, CT, MRI, or PET-CT for staging and re-staging of adults with cutaneous melanoma. The Cochrane database of systematic reviews 60 31260100
2014 Computed tomography (CT) angiography for confirmation of the clinical diagnosis of brain death. The Cochrane database of systematic reviews 57 24683063
2012 Intrapancreatic accessory spleen: CT appearance and differential diagnosis. Abdominal imaging 53 22160284
2009 Pancreatic metastases: CT and MRI findings. Diagnostic and interventional radiology (Ankara, Turkey) 51 20027546
2008 Cancer-testis (CT) antigen expression in medulloblastoma. Cancer immunity 51 18426187
1997 The structure of the gene for murine CTP:phosphocholine cytidylyltransferase, Ctpct. Relationship of exon structure to functional domains and identification of transcriptional start sites and potential upstream regulatory elements. The Journal of biological chemistry 51 9148929
2006 PET/CT imaging of lung cancer. Journal of thoracic imaging 50 16770229
2001 CT findings of leukemic pulmonary infiltration with pathologic correlation. European radiology 50 11868094
1992 Lymphoma of bone, muscle, and skin: CT findings. AJR. American journal of roentgenology 48 1529847
2020 Performance of 68Ga-DOTA-SST PET/CT, octreoscan SPECT/CT and 18F-FDG PET/CT in the detection of culprit tumors causing osteomalacia: a meta-analysis. Nuclear medicine communications 47 32000173
2018 The Cancer/Testes (CT) Antigen HORMAD1 promotes Homologous Recombinational DNA Repair and Radioresistance in Lung adenocarcinoma cells. Scientific reports 47 30333500
2014 DIPNECH: when to suggest this diagnosis on CT. Clinical radiology 46 25465294
2007 Can PET/CT replace separate diagnostic CT for cancer imaging? Optimizing CT protocols for imaging cancers of the chest and abdomen. Journal of nuclear medicine : official publication, Society of Nuclear Medicine 46 17204720
2001 Helical CT of the urinary organs. European radiology 46 11870472
2023 Imaging in metastatic breast cancer, CT, PET/CT, MRI, WB-DWI, CCA: review and new perspectives. Cancer imaging : the official publication of the International Cancer Imaging Society 43 37254225
2018 Imaging features (CT, MRI, MRS, and PET/CT) of primary central nervous system lymphoma in immunocompetent patients. Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology 41 30580380
2001 Focal organizing pneumonia: CT and pathologic findings. Journal of Korean medical science 41 11641525
2008 CT staging of colon cancer. Clinical radiology 40 18996269
2021 Using contrast-enhanced CT and non-contrast-enhanced CT to predict EGFR mutation status in NSCLC patients-a radiomics nomogram analysis. European radiology 39 34807270
2013 SPECT/CT in sentinel node imaging. Nuclear medicine communications 38 23276829
1999 Transcriptional activation of the murine CTP:phosphocholine cytidylyltransferase gene (Ctpct): combined action of upstream stimulatory and inhibitory cis-acting elements. Biochimica et biophysica acta 38 10216289
2014 Mutations in PCYT1A cause spondylometaphyseal dysplasia with cone-rod dystrophy. American journal of human genetics 36 24387991
2020 Lipid-associated PML structures assemble nuclear lipid droplets containing CCTα and Lipin1. Life science alliance 35 32461215
2018 Differential Diagnosis of Sinonasal Lymphoma and Squamous Cell Carcinoma on CT, MRI, and PET/CT. Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery 35 29661053
2017 Aortitis in giant cell arteritis: diagnosis with FDG PET/CT and agreement with CT angiography. Autoimmunity reviews 35 28911987
2011 Lymphomatoid granulomatosis: CT and FDG-PET findings. Korean journal of radiology 35 22043148
2022 PET/CT variants and pitfalls in malignant melanoma. Cancer imaging : the official publication of the International Cancer Imaging Society 31 34983677
2010 PET/CT (and CT) instrumentation, image reconstruction and data transfer for radiotherapy planning. Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 31 20709416
2005 CT features of abdominal plasma cell neoplasms. European radiology 31 16034641
2013 Incidental, subsolid pulmonary nodules at CT: etiology and management. Cancer imaging : the official publication of the International Cancer Imaging Society 30 24061063
2011 Pulmonary Langerhans cell histiocytosis in adults: high-resolution CT-pathology comparisons and evolutional changes at CT. European radiology 30 21311888
2009 CT and PET/CT findings of T-cell lymphoma. AJR. American journal of roentgenology 29 19620431
2021 PET/CT in Multiple Myeloma: Beyond FDG. Frontiers in oncology 28 33569348
2023 PET/CT and SPECT/CT Imaging of HER2-Positive Breast Cancer. Journal of clinical medicine 27 37568284
2015 Palmitate induces cisternal ER expansion via the activation of XBP-1/CCTα-mediated phospholipid accumulation in RAW 264.7 cells. Lipids in health and disease 27 26174230
2010 CCTalpha and CCTdelta chaperonin subunits are essential and required for cilia assembly and maintenance in Tetrahymena. PloS one 27 20502701
2006 CHKA and PCYT1A gene polymorphisms, choline intake and spina bifida risk in a California population. BMC medicine 27 17184542
2018 CT Findings of Acute Small-Bowel Entities. Radiographics : a review publication of the Radiological Society of North America, Inc 24 30207931
2010 PET and PET/CT imaging of skeletal metastases. Cancer imaging : the official publication of the International Cancer Imaging Society 24 20663736
2005 Induction of apoptosis by lipophilic activators of CTP:phosphocholine cytidylyltransferase alpha (CCTalpha). The Biochemical journal 24 16097951
1993 The gene for murine CTP:phosphocholine cytidylyltransferase (Ctpct) is located on mouse chromosome 16. Genomics 24 8307580
1985 Localization of CT beta and C kappa on mouse chromosome 6. Immunogenetics 23 2995250
1980 CT of subacute sclerosing panencephalitis. AJNR. American journal of neuroradiology 23 6779587
2013 Effects of CT-Xp gene knock down in melanoma cell lines. Oncotarget 22 23625514
2008 Combined imaging modalities: PET/CT and SPECT/CT. Health physics 22 18849691
1995 Molybdenum-cofactor deficiency: CT and MR findings. Neuropediatrics 21 7791952
2021 Methylome and transcriptome profiling revealed epigenetic silencing of LPCAT1 and PCYT1A associated with lipidome alterations in polycystic ovary syndrome. Journal of cellular physiology 20 33521992
2019 Diagnostic performance of dual-energy CT and subtraction CT for renal lesion detection and characterization. European radiology 20 31134365
2014 The chaperonin CCTα is required for efficient transcription and replication of rabies virus. Microbiology and immunology 20 25082455
2008 Lung cancer screening with CT. Clinics in chest medicine 20 18267181
2023 Pilot Study of Recurrent Ewing's Sarcoma Management with Vigil/Temozolomide/Irinotecan and Assessment of Circulating Tumor (ct) DNA. Clinical cancer research : an official journal of the American Association for Cancer Research 19 36780200
2018 PET/CT of Dementia. AJR. American journal of roentgenology 19 29949415
2017 Mutations in the PCYT1A gene are responsible for isolated forms of retinal dystrophy. European journal of human genetics : EJHG 19 28272537
2009 14-3-3zeta escorts CCTalpha for calcium-activated nuclear import in lung epithelia. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 19 20007511
2008 Bowel wall thickening in children: CT findings. Radiographics : a review publication of the Radiological Society of North America, Inc 19 18480481
2020 FDG PET/CT and CT Findings of Renal Cell Carcinoma With Sarcomatoid Differentiation. AJR. American journal of roentgenology 18 32755159
2016 18F-FDG PET/CT and Contrast-Enhanced CT of Primary Malignant Tracheal Tumor. Clinical nuclear medicine 18 27055141
2005 Preoperative evaluation of renal anatomy and renal masses with helical CT, 3D-CT and 3D-CT angiography. Diagnostic and interventional radiology (Ankara, Turkey) 18 15795842
1998 CT angiogram sign: incidence and significance in lobar consolidations evaluated by contrast-enhanced CT. AJR. American journal of roentgenology 18 9490960
2022 SARS-CoV-2 cycle threshold (Ct) values predict future COVID-19 cases. Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology 17 35472751
2018 Histone H3.3K27M Mobilizes Multiple Cancer/Testis (CT) Antigens in Pediatric Glioma. Molecular cancer research : MCR 17 29453317
2011 PET/CT imaging in inflammatory myopathies. Annals of the New York Academy of Sciences 17 21718321
2017 CT-P10 (Truxima™): A Rituximab Biosimilar. BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy 16 28497220
2011 CTP:phosphocholine cytidylyltransferase α (CCTα) and lamins alter nuclear membrane structure without affecting phosphatidylcholine synthesis. Biochimica et biophysica acta 16 21504799
2023 CT of the urinary tract revisited. European journal of radiology 15 36773595
2022 Correlation of CT-data derived from multiparametric dual-layer CT-maps with immunohistochemical biomarkers in invasive breast carcinomas. European journal of radiology 15 36219916
2021 Dual-Energy CT Vital Iodine Tumor Burden for Response Assessment in Patients With Metastatic GIST Undergoing TKI Therapy: Comparison With Standard CT and FDG PET/CT Criteria. AJR. American journal of roentgenology 15 34668385
2017 Split-bolus CT urogram: Is less more? Abdominal radiology (New York) 15 28271274
2018 Disease-linked mutations in the phosphatidylcholine regulatory enzyme CCTα impair enzymatic activity and fold stability. The Journal of biological chemistry 14 30559292
2017 DNA double strand break (DSB) induction and cell survival in iodine-enhanced computed tomography (CT). Physics in medicine and biology 14 28703119
2006 Integrated multislice CT and Tc-99m Sestamibi SPECT-CT evaluation of solitary pulmonary nodules. La Radiologia medica 14 16671379
2024 AKT2S128/CCTαS315/319/323-positive cancer-associated fibroblasts (CAFs) mediate focal adhesion kinase (FAK) inhibitors resistance via secreting phosphatidylcholines (PCs). Signal transduction and targeted therapy 13 38280862
2021 CtNorm: Real time PCR cycle of threshold (Ct) normalization algorithm. Journal of microbiological methods 13 34116107
2020 PCYT1A suppresses proliferation and migration via inhibiting mTORC1 pathway in lung adenocarcinoma. Biochemical and biophysical research communications 13 32703435
2014 CT urography and hematuria: a retrospective analysis of 771 patients undergoing CT urography over a 1-year period. Acta radiologica (Stockholm, Sweden : 1987) 13 24973256
2021 Assessment of MR Imaging and CT in Differentiating Hereditary and Nonhereditary Paragangliomas. AJNR. American journal of neuroradiology 12 33985956

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