Affinage

NPTN

Neuroplastin · UniProt Q9Y639

Round 2 corrected
Length
398 aa
Mass
44.4 kDa
Annotated
2026-04-29
105 papers in source corpus 10 papers cited in narrative 10 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

Neuroplastin (NPTN) encodes two immunoglobulin superfamily glycoproteins, Np65 (three Ig domains, brain-specific) and Np55 (two Ig domains, ubiquitous), generated by alternative splicing (PMID:8995369). NPTN functions as an obligatory subunit of plasma membrane Ca²⁺-ATPases (PMCAs), with its single transmembrane helix contacting PMCA1 TM8–9 linker and TM10 to enable Ca²⁺ clearance, as demonstrated by cryo-EM at 4.1 Å resolution (PMID:30190470); in cochlear outer hair cells, Np55 is specifically required for PMCA2 membrane localization, mechanotransduction, and tectorial membrane coupling, and its loss causes deafness in mice (PMID:27581460, PMID:35100259). NPTN also serves as a chaperone that delivers the Xkr8 phospholipid scramblase to the plasma membrane, forming a heterocomplex essential for apoptotic phosphatidylserine exposure (PMID:27503893). At synapses, Np65 mediates trans-homophilic adhesion activating p38 MAPK signaling and GluR1 internalization, binds GABA-A receptor subunits to regulate inhibitory synapse composition, and activates FGFR signaling (PMID:25040546).

Mechanistic history

Synthesis pass · year-by-year structured walk · 6 steps
  1. 1997 High

    Molecular cloning established that the synaptic glycoproteins gp65 and gp55 are immunoglobulin superfamily members produced by alternative splicing from a single gene, resolving the identity and domain architecture of Neuroplastin isoforms.

    Evidence cDNA library screening, heterologous expression in HEK293 cells with tunicamycin deglycosylation, Northern blot, and immunoprecipitation

    PMID:8995369

    Open questions at the time
    • No binding partners or functional roles identified beyond structural characterization
    • Mechanism of tissue-specific splicing regulation unknown
  2. 1998 Medium

    Tissue-specific glycosylation was shown to depend on the expressing cell type rather than the isoform, establishing that post-translational modification diversity of Neuroplastin is host-cell-determined.

    Evidence Deglycosylation assays and HEK cell transfection with isoform-specific constructs

    PMID:9662433

    Open questions at the time
    • Functional consequences of differential glycosylation remain unresolved
    • Single-lab observation without independent replication
  3. 2014 Medium

    A body of work revealed that Neuroplastin participates in synaptic regulation through multiple mechanisms: Np65 trans-homophilic binding activates p38 MAPK and drives GluR1 internalization, both isoforms act as monocarboxylate transporter accessories, and both bind GABA-A receptor subunits, placing NPTN at the intersection of excitatory/inhibitory synapse regulation and neuronal metabolism.

    Evidence Co-immunoprecipitation, cell adhesion assays, electrophysiology, receptor internalization assays across multiple primary studies (compiled in review)

    PMID:25040546

    Open questions at the time
    • Structural basis of Np65 trans-homophilic interaction unknown
    • Relative contributions of FGFR vs. p38 MAPK arms to synaptic plasticity not dissected
    • Evidence compiled from review; individual findings vary in rigor
  4. 2016 High

    Two parallel discoveries revealed unexpected non-synaptic roles: NPTN was shown to be an obligatory subunit of the Xkr8 phospholipid scramblase complex required for apoptotic PS exposure, and Np55 was found to be essential for outer hair cell stereocilia function and cochlear amplification, broadening the gene's functional scope beyond the nervous system.

    Evidence BSG⁻/⁻NPTN⁻/⁻ double-knockout cells with scramblase assays and mutagenesis (Xkr8); ENU mutagenesis screen with in vivo vibrometry and cochlear microphonics (hearing)

    PMID:27503893 PMID:27581460

    Open questions at the time
    • Stoichiometry and assembly pathway of the Xkr8–NPTN complex not fully resolved
    • Whether NPTN versus BSG preferentially partners with Xkr8 in specific tissues is unknown
    • Molecular basis of tectorial membrane coupling via Np55 not identified
  5. 2018 High

    Cryo-EM structure of the hPMCA1–NPTN complex at near-atomic resolution revealed the direct transmembrane interface and established NPTN as an obligatory PMCA subunit that opens the cytoplasmic Ca²⁺ entry pathway, providing the first structural explanation for NPTN-dependent Ca²⁺ clearance.

    Evidence Cryo-EM at 4.1 Å resolution with functional Ca²⁺-ATPase activity assays

    PMID:30190470

    Open questions at the time
    • Structures of NPTN with PMCA2, PMCA3, or PMCA4 not determined
    • How NPTN binding shifts the conformational cycle of PMCAs beyond the E1-Mg²⁺ state is unresolved
    • Role of NPTN Ig ectodomains in PMCA regulation not addressed structurally
  6. 2022 High

    Isoform-specific knockout mice demonstrated that Np55, not Np65, is the functionally essential isoform for hearing; continued adult expression is required for PMCA2 membrane localization in OHCs, and NPTN haploinsufficiency genetically interacts with the Cdh23ahl deafness allele, directly linking NPTN dosage to hearing loss.

    Evidence Isoform-specific knockout mice, ABR thresholds, whole-cell patch-clamp for MET currents, PMCA2 immunofluorescence, genetic interaction with Cdh23ahl

    PMID:35100259

    Open questions at the time
    • Mechanism by which NPTN maintains PMCA2 at the stereocilia membrane is unknown
    • Whether NPTN haploinsufficiency contributes to age-related hearing loss in humans is untested
    • How loss of NPTN impairs MET channel open probability independently of PMCA2 is unexplained

Open questions

Synthesis pass · forward-looking unresolved questions
  • The structural basis of Np65 trans-homophilic adhesion, the mechanism by which NPTN maintains PMCA2 at stereocilia membranes in adulthood, and the tissue-specific logic determining whether NPTN or BSG partners with Xkr8 and PMCAs remain unresolved.
  • No crystal or cryo-EM structure of Np65 Ig1 domain in trans-homophilic configuration
  • No reconstitution of NPTN–PMCA2 complex for structural or functional analysis
  • Functional role of NPTN in immune cells beyond cochlea and brain lacks rigorous primary data

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0044183 protein folding chaperone 2 GO:0098631 cell adhesion mediator activity 2 GO:0098772 molecular function regulator activity 2
Localization
GO:0005886 plasma membrane 3
Pathway
R-HSA-112316 Neuronal System 3 GO:0005198 structural molecule activity 2 R-HSA-162582 Signal Transduction 2 R-HSA-382551 Transport of small molecules 2 R-HSA-9709957 Sensory Perception 2 R-HSA-5357801 Programmed Cell Death 1
Partners
ATP2B1ATP2B2BSGFGFR1GABRA1XKR8
Complex memberships
PMCA1–NPTN complexPMCA2–NPTN complexXkr8–NPTN scramblase complex

Evidence

Reading pass · 10 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 Synaptic membrane glycoproteins gp65 and gp55 (rat Neuroplastin isoforms) were identified as members of the immunoglobulin superfamily, produced by alternative splicing of a single gene transcript. cDNA cloning revealed open reading frames of 397 and 281 amino acids containing three and two Ig domains respectively, with ~40% sequence identity to neurothelin/basigin. Expression in HEK293 cells with tunicamycin confirmed the core protein sizes match deglycosylated gp65 and gp55. Northern analysis showed gp65 is brain-specific while gp55 is ubiquitously expressed. cDNA library screening, heterologous expression in HEK293 cells, tunicamycin deglycosylation, Northern blot, in situ hybridization, immunoprecipitation The Journal of biological chemistry High 8995369
1998 Gp65 and gp55 are produced by alternative splicing of the same gene and exhibit tissue-specific glycoforms. Deglycosylation studies demonstrated that outside the brain, all immunoreactive species derive from the gp55 isoform. Transfection of HEK cells with gp65 or gp55 cDNAs showed that glycosylation patterns depend on the cell type expressing these molecules, not solely on the isoform. Monoclonal antibody detection, concanavalin A binding, deglycosylation assays, HEK cell transfection FEBS letters Medium 9662433
2014 Neuroplastin Np65 and Np55 play roles in neurite outgrowth, regulation of inhibitory and excitatory synapses, and synaptic plasticity. Mechanistically, Np65 undergoes trans-homophilic binding leading to activation of p38 MAPK and internalization of GluR1 glutamate receptor subunits; both isoforms act as accessory proteins for monocarboxylate transporters affecting neuronal energy supply; and both bind GABA-A receptor α1, 2, and 5 subunits, regulating GABA-A receptor composition and localization. Neuroplastins also bind to and activate the fibroblast growth factor receptor (FGFR). Co-immunoprecipitation, cell adhesion assays, electrophysiology, receptor internalization assays, binding studies (review summarizing experimental findings from multiple primary studies) Journal of neurochemistry Medium 25040546
2016 Neuroplastin (NPTN) and basigin (BSG) were identified as obligatory subunits of the Xkr8 phospholipid scramblase complex. In BSG−/−NPTN−/− double-knockout cells, Xkr8 was retained intracellularly and failed to execute phosphatidylserine exposure upon apoptotic stimulation, demonstrating that BSG and NPTN chaperone Xkr8 to the plasma membrane. Upon apoptotic signaling, Xkr8 is cleaved at its C-terminus and the Xkr8/BSG or Xkr8/NPTN complex assembles into a higher-order complex (likely a heterotetramer of two Xkr8 and two BSG/NPTN molecules) required for scrambling activity. Mutational analysis of BSG showed that an atypical glutamic acid in its transmembrane region is required for association with Xkr8. Double-knockout cell lines (BSG−/−NPTN−/−), subcellular localization assays, phosphatidylserine exposure assay, co-immunoprecipitation, transmembrane domain mutagenesis Proceedings of the National Academy of Sciences of the United States of America High 27503893
2016 Neuroplastin isoform Np55 is specifically expressed in stereocilia of outer hair cells (OHCs) but not inner hair cells. A point mutation in Nptn identified by ENU mutagenesis causes deafness in mice. Np55 is required for stable mechanical coupling of OHC stereocilia with the tectorial membrane; mutant mice lack cochlear amplification as shown by in vivo vibrometry. Hair bundles develop morphological defects with age and mechanotransduction is impaired. This reveals functional diversification between Np55 (OHC stereocilia function) and Np65 (synaptic regulation). ENU mutagenesis screen, in vivo vibrometry, immunofluorescence localization, cochlear microphonics recording, morphological analysis of hair bundles The Journal of neuroscience High 27581460
2018 Cryo-EM structure of human PMCA1 (hPMCA1) in complex with NPTN was solved at 4.1 Å overall (3.9 Å for the transmembrane domain). The single transmembrane helix of NPTN interacts with the TM8-9 linker and TM10 of hPMCA1. NPTN is required for hPMCA1 functional activity (Ca2+-ATPase function), and the NPTN-bound hPMCA1 resembles the E1-Mg2+ state of SERCA with an open cytoplasmic Ca2+ entry pathway. This established NPTN as an obligatory subunit of plasma membrane Ca2+-ATPases that dramatically increases efficiency of PMCA-mediated Ca2+ clearance. Cryo-EM structure determination, functional Ca2+-ATPase activity assay, transmembrane domain interaction analysis Nature communications High 30190470
2022 Np55 is the functionally sufficient isoform for cochlear hair cell function and hearing. Np65-specific knockout mice have normal hearing thresholds, demonstrating Np65 is functionally redundant for hearing. Nptn-knockout OHCs have significantly reduced maximal MET currents and MET channel open probabilities, and both OHCs and IHCs fail to develop mature basolateral currents. Continued Neuroplastin expression in adult OHCs is required for membrane localization of PMCA2 (encoded by Atp2b2), which is essential for hearing. Nptn haploinsufficiency phenocopies Atp2b2 mutations and shows genetic interaction with the Cdh23ahl allele. Isoform-specific knockout mice, auditory brainstem response (ABR) threshold measurement, whole-cell patch-clamp electrophysiology (MET currents), immunofluorescence for PMCA2 localization, genetic interaction analysis PLoS genetics High 35100259
2022 Neuroplastin Np55 and Np65 isoforms are present in cochlear cells, and the role of Neuroplastin in macrophages during bacterial infection was attributed to one of the two isoforms. Critical re-analysis indicates that assignment of macrophage roles requires distinguishing between Np55 and Np65 splice variants, as the two isoforms have distinct expression patterns and likely distinct functions in innate immune cells. Isoform-specific expression analysis, critical review of experimental evidence Molecular immunology Low 38615628
2015 NPTN was identified as a component of the human interactome via high-throughput affinity-purification mass spectrometry (AP-MS) in HEK293T cells (BioPlex network), revealing protein-protein interaction partners for NPTN within the cellular proteome. Affinity-purification mass spectrometry (AP-MS), high-throughput interactome mapping Cell Low 26186194
2021 Proteome-scale BioPlex 3.0 interaction network confirmed NPTN interaction partners in both HEK293T and HCT116 cell lines via AP-MS, providing cell-line-specific interaction data for NPTN. Affinity-purification mass spectrometry (AP-MS) in two cell lines Cell Low 33961781

Source papers

Stage 0 corpus · 105 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proceedings of the National Academy of Sciences of the United States of America 1479 12477932
2015 The BioPlex Network: A Systematic Exploration of the Human Interactome. Cell 1118 26186194
2020 A reference map of the human binary protein interactome. Nature 849 32296183
2021 Dual proteome-scale networks reveal cell-specific remodeling of the human interactome. Cell 705 33961781
2012 A census of human soluble protein complexes. Cell 689 22939629
2011 Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Briefings in bioinformatics 656 21873635
2018 The shieldin complex mediates 53BP1-dependent DNA repair. Nature 502 30022168
1990 Activation of cell growth by binding of Friend spleen focus-forming virus gp55 glycoprotein to the erythropoietin receptor. Nature 448 2154701
2004 The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome research 438 15489334
2022 OpenCell: Endogenous tagging for the cartography of human cellular organization. Science (New York, N.Y.) 432 35271311
2018 DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of NHEJ and PARP Inhibitor Sensitivity. Cell 379 29656893
2021 A proximity-dependent biotinylation map of a human cell. Nature 339 34079125
2007 Huntingtin interacting proteins are genetic modifiers of neurodegeneration. PLoS genetics 325 17500595
1990 Cell surface expression of human cytomegalovirus (HCMV) gp55-116 (gB): use of HCMV-recombinant vaccinia virus-infected cells in analysis of the human neutralizing antibody response. Journal of virology 278 2154594
2004 Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation. Nature biotechnology 266 15146197
2011 A directed protein interaction network for investigating intracellular signal transduction. Science signaling 258 21900206
1990 Friend spleen focus-forming virus glycoprotein gp55 interacts with the erythropoietin receptor in the endoplasmic reticulum and affects receptor metabolism. Proceedings of the National Academy of Sciences of the United States of America 240 2161534
2008 Systematic identification of mRNAs recruited to argonaute 2 by specific microRNAs and corresponding changes in transcript abundance. PloS one 148 18461144
2019 The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2. Molecular cell 124 30639242
1986 Synthesis and processing of the envelope gp55-116 complex of human cytomegalovirus. Journal of virology 121 3005648
2017 An Interaction Landscape of Ubiquitin Signaling. Molecular cell 119 28190767
2016 Xkr8 phospholipid scrambling complex in apoptotic phosphatidylserine exposure. Proceedings of the National Academy of Sciences of the United States of America 117 27503893
1983 Complete nucleotide sequence of an infectious clone of Friend spleen focus-forming provirus: gp55 is an envelope fusion glycoprotein. Proceedings of the National Academy of Sciences of the United States of America 115 6576374
2021 Protein interaction landscapes revealed by advanced in vivo cross-linking-mass spectrometry. Proceedings of the National Academy of Sciences of the United States of America 113 34349018
1989 Processing of the gp55-116 envelope glycoprotein complex (gB) of human cytomegalovirus. Journal of virology 113 2535741
2009 Quantitative proteomics identifies a Dab2/integrin module regulating cell migration. The Journal of cell biology 101 19581412
1988 Induction of complement-dependent and -independent neutralizing antibodies by recombinant-derived human cytomegalovirus gp55-116 (gB). Journal of virology 97 2841483
1990 Activation of erythropoietin receptors by Friend viral gp55 and by erythropoietin and down-modulation by the murine Fv-2r resistance gene. Proceedings of the National Academy of Sciences of the United States of America 95 2175917
1991 Distribution of linear antigenic sites on glycoprotein gp55 of human cytomegalovirus. Journal of virology 93 1702157
2004 Signal peptide prediction based on analysis of experimentally verified cleavage sites. Protein science : a publication of the Protein Society 92 15340161
1983 Complete nucleotide sequence of the gene for the specific glycoprotein (gp55) of Friend spleen focus-forming virus. Proceedings of the National Academy of Sciences of the United States of America 92 6306650
2017 The STUbL RNF4 regulates protein group SUMOylation by targeting the SUMO conjugation machinery. Nature communications 86 29180619
2020 Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains. Cell reports 79 32814053
1992 The erythropoietin receptor transmembrane region is necessary for activation by the Friend spleen focus-forming virus gp55 glycoprotein. Molecular and cellular biology 77 1320192
2020 TGFβ attenuates cartilage extracellular matrix degradation via enhancing FBXO6-mediated MMP14 ubiquitination. Annals of the rheumatic diseases 75 32409323
2018 Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin. Nature communications 75 30190470
2014 The Neuroplastin adhesion molecules: key regulators of neuronal plasticity and synaptic function. Journal of neurochemistry 73 25040546
1997 Synaptic membrane glycoproteins gp65 and gp55 are new members of the immunoglobulin superfamily. The Journal of biological chemistry 71 8995369
1996 Characterization of mouse ZP3-derived glycopeptide, gp55, that exhibits sperm receptor and acrosome reaction-inducing activity in vitro. Biochemistry 67 8672430
2021 Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy. Acta pharmaceutica Sinica. B 66 35256949
2000 Vaccination with a single dose of a recombinant porcine adenovirus expressing the classical swine fever virus gp55 (E2) gene protects pigs against classical swine fever. Vaccine 61 10590324
1990 Env-derived gp55 gene of Friend spleen focus-forming virus specifically induces neoplastic proliferation of erythroid progenitor cells. The EMBO journal 61 2162763
2017 Systematic protein-protein interaction mapping for clinically relevant human GPCRs. Molecular systems biology 58 28298427
1989 Biosynthesis, structure, and biological activities of envelope protein gp65 of murine coronavirus. Virology 54 2556847
1996 A "double adaptor" method for improved shotgun library construction. Analytical biochemistry 53 8619474
1994 A dominant negative erythropoietin (EPO) receptor inhibits EPO-dependent growth and blocks F-gp55-dependent transformation. Molecular and cellular biology 53 8139531
2002 A bipartite bacteriophage T4 SOC and HOC randomized peptide display library: detection and analysis of phage T4 terminase (gp17) and late sigma factor (gp55) interaction. Journal of molecular biology 49 12051907
2014 Single nucleotide polymorphism in the neuroplastin locus associates with cortical thickness and intellectual ability in adolescents. Molecular psychiatry 48 24514566
1999 Activation of the erythropoietin receptor by the gp55-P viral envelope protein is determined by a single amino acid in its transmembrane domain. The EMBO journal 43 10369674
2005 Immunomodulatory effect of plasmids co-expressing cytokines in classical swine fever virus subunit gp55/E2-DNA vaccination. Veterinary research 41 15955282
1992 Activation of the erythropoietin receptor by the Friend spleen focus-forming virus gp55 glycoprotein induces constitutive protein tyrosine phosphorylation. Blood 40 1467516
1985 The mature form of the Friend spleen focus-forming virus envelope protein, gp65, is efficiently secreted from cells. Virology 38 2998032
1995 Cell surface activation of the erythropoietin receptor by Friend spleen focus-forming virus gp55. Journal of virology 33 7853508
1989 Development of metacyclic Leishmania promastigotes is associated with the increasing expression of GP65, the major surface antigen. Parasite immunology 33 2771426
1993 Isolation and characterization of a major allergenic component (gp55) of Aspergillus fumigatus. The Journal of allergy and clinical immunology 32 8227861
2000 Protection of pigs against classical swine fever with DNA-delivered gp55. Vaccine 31 10699343
1998 Immunoglobulin superfamily members gp65 and gp55: tissue distribution of glycoforms. FEBS letters 31 9662433
1976 Cellular hypersensitivity of gp55 of RIII-murine mammary tumor virus and gp55-like protein of human breast cancers. Cancer research 31 184925
1993 Fusion of the erythropoietin receptor and the Friend spleen focus-forming virus gp55 glycoprotein transforms a factor-dependent hematopoietic cell line. Molecular and cellular biology 30 8423798
1989 Requirement of the single base insertion at the 3' end of the env-related gene of Friend spleen focus-forming virus for pathogenic activity and its effect on localization of the glycoprotein product (gp55). Journal of virology 29 2552155
1996 A family of glycoproteins (GP55), which inhibit neurite outgrowth, are members of the Ig superfamily and are related to OBCAM, neurotrimin, LAMP and CEPU-1. Journal of cell science 28 9004047
1998 The anemic Friend virus gp55 envelope protein induces erythroid differentiation in fetal liver colony-forming units-erythroid. Blood 26 9454745
2016 Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function. The Journal of neuroscience : the official journal of the Society for Neuroscience 25 27581460
1998 Erythropoietin and Friend virus gp55 activate different JAK/STAT pathways through the erythropoietin receptor in erythroid cells. Molecular and cellular biology 25 9488432
2001 Oral and sub-cutaneous vaccination of commercial pigs with a recombinant porcine adenovirus expressing the classical swine fever virus gp55 gene. Archives of virology 24 11699963
2003 The erythropoietin receptor transmembrane domain mediates complex formation with viral anemic and polycythemic gp55 proteins. The Journal of biological chemistry 23 12930840
1991 Characterization of a major antigenic region on gp55 of human cytomegalovirus. The Journal of general virology 23 1662693
1990 A deletion in the Friend spleen focus-forming virus env gene is necessary for its product (gp55) to be leukemogenic. Journal of virology 20 2159537
1987 Expression of the human monocyte membrane antigen gp55 by murine fibroblasts after DNA-mediated gene transfer. Blood 20 3545322
2005 Helix packing and orientation in the transmembrane dimer of gp55-P of the spleen focus forming virus. Biophysical journal 19 15894629
1990 Structural and immunological characterization of human cytomegalovirus gp55-116 (gB) expressed in insect cells. The Journal of general virology 19 2157800
1993 Cloning and nucleotide sequence determination of the major envelope glycoprotein (gp55) gene of hog cholera virus (Weybridge). Virus research 18 8317145
1980 Analysis of spleen focus-forming virus-specific RNA sequences coding for spleen focus-forming virus-specific glycoprotein with a molecular weight of 55,000 (gp55). Journal of virology 18 6774106
1994 Selectable retrovirus vectors encoding Friend virus gp55 or erythropoietin induce polycythemia with different phenotypic expression and disease progression. Journal of virology 17 7933106
1989 Aldosterone-induced proteins: purification and localization of GP65,70. The American journal of physiology 17 2495728
1984 env-Related leukemogenic genes (gp55 genes) of two closely related polycythemic strains of Friend spleen focus-forming virus possess different recombination points with an endogenous mink cell focus-forming virus env gene. Virology 17 6464355
2002 Mutational and functional analysis of a segment of the sigma family bacteriophage T4 late promoter recognition protein gp55. The Journal of biological chemistry 16 12496274
1997 GP55 inhibits both cell adhesion and growth of neurons, but not non-neuronal cells, via a G-protein-coupled receptor. The European journal of neuroscience 16 9058053
1992 The interaction of the erythropoietin receptor and gp55. Cancer surveys 15 1451111
2016 Single Chain Antibodies Against gp55 of Human Cytomegalovirus (HCMV) for Prophylaxis and Treatment of HCMV Infections. Jundishapur journal of microbiology 12 27217918
2022 Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing. PLoS genetics 11 35100259
2009 Activation of the N-terminally truncated form of the Stk receptor tyrosine kinase Sf-Stk by Friend virus-encoded gp55 is mediated by cysteine residues in the ecotropic domain of gp55 and the extracellular domain of Sf-Stk. Journal of virology 11 20016000
1998 Role of the transmembrane sequence of spleen focus-forming virus gp55 in erythroleukemogenesis. Virology 10 9875316
2023 Trypanosoma brucei Invariant Surface gp65 Inhibits the Alternative Pathway of Complement by Accelerating C3b Degradation. Journal of immunology (Baltimore, Md. : 1950) 9 37466368
1995 Both the changes of six amino acids and the C-terminal truncation caused by a one-base insertion in the defective env gene of Friend spleen focus-forming virus significantly affect the pathogenic activity of the encoded leukemogenic membrane glycoprotein (gp55). Journal of virology 9 7494268
1993 Erythropoietin receptor binds to Friend virus gp55 through other membrane components. Biochemical and biophysical research communications 9 8507186
1993 The role of gp55 N-glycosylation in pathogenesis of Friend spleen focus-forming virus. The Journal of general virology 8 8468555
2022 LncRNA KCNQ1OT1 promotes the apoptosis and inflammatory response of microglia by regulating the miR-589-5p/NPTN axis after spinal cord injury. Anais da Academia Brasileira de Ciencias 7 35703693
2023 Durable response to afatinib in advanced lung adenocarcinoma harboring a novel NPTN-NRG1 fusion: a case report. World journal of surgical oncology 6 37587479
1998 Abortive initiation of transcription at a hybrid promoter. An analysis of the sliding clamp activator of bacteriophage T4 late transcription, and a comparison of the sigma70 and T4 gp55 promoter recognition proteins. The Journal of biological chemistry 6 9852060
1997 Activation of the JAK1-STAT5 pathway by binding of the Friend virus gp55 glycoprotein to the erythropoietin receptor. Leukemia 6 9209415
1996 Tumor necrosis factor-induced expression of porcine glycoproteins gp65 and gp100 recognized by human xenoreactive natural antibodies. Transplantation 6 8779689
1995 A mouse erythroleukemia cell line possessing friend spleen focus-forming virus gp55 transgene and temperature-sensitive mutant p53 gene. Japanese journal of cancer research : Gann 6 7744699
1985 Purification and characterization of GP-55, a protein associated with actin-based cytoplasmic gels derived from brain tissue. The Journal of biological chemistry 6 3891758
2024 LncRNA NPTN-IT1-201 Ameliorates Depressive-like Behavior by Targeting miR-142-5p and Regulating Inflammation and Apoptosis via BDNF. Current medical science 5 39145838
1998 Role of gp55 in restoring the sensitivity of Friend murine erythroleukemia cells to erythropoietin by exposure to dimethyl sulfoxide. Oncology research 3 9778688
1998 Sequence flexibility in the polytropic env gp70-derived region of the membrane glycoprotein (gp55) of Friend spleen focus-forming virus affects its biological activity. Journal of virology 2 9499086
1998 Impaired activation and binding of the erythropoietin receptor by a mutant gp55 of Friend spleen focus-forming virus, which has a cytoplasmic domain. Virology 2 9657942
2024 A novel transcription factor Sdr1 involving sulfur depletion response in fission yeast. Genes to cells : devoted to molecular & cellular mechanisms 1 39105351
2001 Epitope mapping of human herpesvirus-7 gp65 using monoclonal antibodies. Archives of virology 1 11699957
1998 Pathogenicity of a mutant friend spleen focus-forming virus encoding an Env-like membrane glycoprotein (gp55) with substitution by a xenotropic murine leukemia virus Env gp70 sequence. Microbiology and immunology 1 9623923
2026 The SDR1-OsDSK2a-EUI1 module orchestrates plant height and multi-stress resilience in rice. The Plant cell 0 42013295
2024 Neuroplastin splice variants Np55 and Np65: Who is doing the job in macrophages? Molecular immunology 0 38615628
2008 [Preparation and characteristics of monoclonal antibodies to protein E2 (GP55) of classical hog cholera virus]. Voprosy virusologii 0 19069792
1997 Mutational analysis of the structure-function relationship of the leukemogenic membrane glycoprotein (GP55) of Friend spleen focus-forming virus (F-SFFV). Leukemia 0 9209329