Affinage

NIM1K

Serine/threonine-protein kinase NIM1 · UniProt Q8IY84

Length
436 aa
Mass
49.6 kDa
Annotated
2026-04-29
33 papers in source corpus 1 papers cited in narrative 1 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NIM1K is a serine/threonine-protein kinase that localizes inside the primary cilium in a prenylation-dependent manner, with its ciliary trafficking mediated by the prenylated-cargo chaperone PDE6D (PMID:36672247). The kinase substrates and signaling functions of NIM1K within the cilium have not been established.

Mechanistic history

Synthesis pass · year-by-year structured walk · 1 step
  1. 2023 Medium

    Identifying NIM1K as a prenylated ciliary protein resolved how this kinase reaches its subcellular destination: PDE6D binds NIM1K as a prenylated cargo and is required for its ciliary localization.

    Evidence Affinity proteomics (PDE6D pulldown), ciliary imaging, and prenylation-dependence assays in human cells

    PMID:36672247

    Open questions at the time
    • Single study; ciliary localization of NIM1K has not been independently confirmed
    • No kinase substrates or downstream signaling pathway identified
    • Functional consequence of NIM1K loss from the cilium is unknown

Open questions

Synthesis pass · forward-looking unresolved questions
  • The catalytic targets and physiological role of NIM1K kinase activity within the primary cilium remain entirely undefined.
  • No substrate identified for NIM1K kinase activity
  • No loss-of-function phenotype reported in any model organism
  • Relationship to ciliopathy-associated signaling pathways not investigated

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 1
Localization
GO:0005929 cilium 1
Partners
PDE6D

Evidence

Reading pass · 1 per-paper finding extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2023 Human NIM1K (serine/threonine-protein kinase NIM1) was identified as a prenylated cargo protein of PDE6D; NIM1K localizes inside the primary cilium in a prenylation-dependent manner, and this trafficking is mediated by the ciliopathy protein PDE6D. Affinity proteomics (PDE6D pulldown/interactome), subcellular localization by imaging, prenylation-dependent localization assay Cells Medium 36672247

Source papers

Stage 0 corpus · 33 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2002 Tobacco Rar1, EDS1 and NPR1/NIM1 like genes are required for N-mediated resistance to tobacco mosaic virus. The Plant journal : for cell and molecular biology 781 12028572
2000 The Arabidopsis NPR1/NIM1 protein enhances the DNA binding activity of a subgroup of the TGA family of bZIP transcription factors. The Plant cell 367 10662863
1997 The Arabidopsis NIM1 protein shows homology to the mammalian transcription factor inhibitor I kappa B. The Plant cell 326 9090885
1987 The mitotic inducer nim1+ functions in a regulatory network of protein kinase homologs controlling the initiation of mitosis. Cell 293 3453113
1999 Nim1-related kinases coordinate cell cycle progression with the organization of the peripheral cytoskeleton in yeast. Genes & development 275 9925642
2002 Benzothiadiazole-induced priming for potentiated responses to pathogen infection, wounding, and infiltration of water into leaves requires the NPR1/NIM1 gene in Arabidopsis. Plant physiology 178 11891259
1993 Negative regulation of the wee1 protein kinase by direct action of the nim1/cdr1 mitotic inducer. Cell 164 7681363
1993 Nim1 kinase promotes mitosis by inactivating Wee1 tyrosine kinase. Nature 143 8515818
1999 Harpin induces disease resistance in Arabidopsis through the systemic acquired resistance pathway mediated by salicylic acid and the NIM1 gene. The Plant journal : for cell and molecular biology 142 10571880
1993 Phosphorylation and inactivation of the mitotic inhibitor Wee1 by the nim1/cdr1 kinase. Nature 140 8515817
2001 NIMIN-1, NIMIN-2 and NIMIN-3, members of a novel family of proteins from Arabidopsis that interact with NPR1/NIM1, a key regulator of systemic acquired resistance in plants. Plant molecular biology 91 11442055
2002 Over-expression of TGA5, which encodes a bZIP transcription factor that interacts with NIM1/NPR1, confers SAR-independent resistance in Arabidopsis thaliana to Peronospora parasitica. The Plant journal : for cell and molecular biology 90 12383081
1991 Genetic and molecular analysis of cdr1/nim1 in Schizosaccharomyces pombe. Genetics 90 2004705
2004 In Candida albicans, the Nim1 kinases Gin4 and Hsl1 negatively regulate pseudohypha formation and Gin4 also controls septin organization. The Journal of cell biology 85 14769857
1998 The protein kinase Cdr2, related to Nim1/Cdr1 mitotic inducer, regulates the onset of mitosis in fission yeast. Molecular biology of the cell 84 9843572
2002 Runaway cell death, but not basal disease resistance, in lsd1 is SA- and NIM1/NPR1-dependent. The Plant journal : for cell and molecular biology 77 11844114
2001 NIM1 overexpression in Arabidopsis potentiates plant disease resistance and results in enhanced effectiveness of fungicides. Molecular plant-microbe interactions : MPMI 66 11551076
2001 Abnormal callose response phenotype and hypersusceptibility to Peronospoara parasitica in defence-compromised arabidopsis nim1-1 and salicylate hydroxylase-expressing plants. Molecular plant-microbe interactions : MPMI 63 11310731
2002 Role of salicylic acid and NIM1/NPR1 in race-specific resistance in arabidopsis. Genetics 55 12072475
2006 Direct phosphorylation and activation of a Nim1-related kinase Gin4 by Elm1 in budding yeast. The Journal of biological chemistry 51 16861226
1996 Spatial organization of the Nim1-Wee1-Cdc2 mitotic control network in Schizosaccharomyces pombe. Molecular biology of the cell 33 8930897
1996 Nik1: a Nim1-like protein kinase of S. cerevisiae interacts with the Cdc28 complex and regulates cell cycle progression. Genes to cells : devoted to molecular & cellular mechanisms 32 9077450
2012 CDK regulates septin organization through cell-cycle-dependent phosphorylation of the Nim1-related kinase Gin4. Journal of cell science 25 22366454
1997 Roles of Wee1 and Nim1 protein kinases in regulating the switch from mitotic division to sexual development in Schizosaccharomyces pombe. Molecular and cellular biology 21 8972180
2001 Salicylic acid and NIM1/NPR1-independent gene induction by incompatible Peronospora parasitica in arabidopsis. Molecular plant-microbe interactions : MPMI 16 11605963
1999 Interleukin-1alpha regulates G1 cell cycle progression and arrest in thyroid carcinoma cell lines NIM1 and NPA. The Journal of endocrinology 15 9854178
2016 The Nim1 kinase Gin4 has distinct domains crucial for septin assembly, phospholipid binding and mitotic exit. Journal of cell science 13 27231094
2019 A mechanism for how Cdr1/Nim1 kinase promotes mitotic entry by inhibiting Wee1. Molecular biology of the cell 10 31644361
2023 PDE6D Mediates Trafficking of Prenylated Proteins NIM1K and UBL3 to Primary Cilia. Cells 7 36672247
2024 The Vitis yeshanensis U-box E3 ubiquitin ligase VyPUB21 enhances resistance to powdery mildew by targeting degradation of NIM1-interacting (NIMIN) protein. Plant cell reports 4 38467927
1999 Interaction between the fission yeast nim1/cdr1 protein kinase and a dynamin-related protein. FEBS letters 4 9928955
1995 The fission yeast Nim1/Cdr1 kinase: a link between nutritional state and cell cycle control. Progress in cell cycle research 4 9552364
1997 Evidence for a mammalian Nim1-like kinase pathway acting at the G0-1/S transition. Biochemical and biophysical research communications 3 9223439