Affinage

NETO2

Neuropilin and tolloid-like protein 2 · UniProt Q8NC67

Length
525 aa
Mass
59.4 kDa
Annotated
2026-04-29
32 papers in source corpus 18 papers cited in narrative 18 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

NETO2 is a single-pass transmembrane auxiliary subunit of kainate-type glutamate receptors (KARs) that also stabilizes the K⁺-Cl⁻ cotransporter KCC2, thereby coordinating excitatory receptor gating and inhibitory chloride homeostasis in the nervous system. As a KAR auxiliary subunit, NETO2 co-assembles with GluK1–GluK5 subunit combinations at variable stoichiometry (one or two copies per tetramer) and slows channel opening ~7-fold and closing ~3-fold, slows desensitization, accelerates recovery from desensitization, and reduces polyamine-dependent inward rectification; cryo-EM structures show that NETO2 crosslinks the ATD and LBD layers of the receptor and its transmembrane helix competes with the post-M4 amphipathic helix for an intracellular cap, explaining gating and rectification modulation, while during activation NETO2 prevents tightening of the inter-LBD-dimer interface to slow deactivation (PMID:21632929, PMID:22973017, PMID:26282342, PMID:34552241, PMID:40846810, PMID:41197725). NETO2 controls synaptic KAR abundance by bridging KARs to the scaffolding protein GRIP and is regulated by CaMKII/PKA phosphorylation at Ser-409, which restricts GluK1 synaptic targeting; loss of NETO2 depletes KARs from postsynaptic densities across multiple brain regions, impairs PV interneuron maturation, alters fear learning, and causes seizure susceptibility (PMID:23236500, PMID:28717010, PMID:30770891, PMID:32788298). Independently of KARs, NETO2 binds and stabilizes the active oligomeric form of KCC2; its loss reduces KCC2 surface expression and Ser-940 phosphorylation, depolarizes the GABA reversal potential, and diminishes synaptic inhibition in hippocampal neurons (PMID:23401525, PMID:26441539).

Mechanistic history

Synthesis pass · year-by-year structured walk · 15 steps
  1. 2011 High

    Establishing NETO2 as a KAR auxiliary subunit answered whether kainate receptors, like AMPA receptors, possess modulatory transmembrane partners — NETO2 slowed desensitization, deactivation, and recovery kinetics across multiple GluK subunit combinations.

    Evidence Rapid glutamate application to outside-out patches from heterologous cells expressing various KAR subunit combinations ± NETO2

    PMID:21632929

    Open questions at the time
    • Structural basis of NETO2–KAR interaction unknown at this stage
    • Relative contribution of NETO2 vs NETO1 to native KAR currents unresolved
  2. 2012 High

    Domain dissection resolved how a single auxiliary subunit exerts two mechanistically distinct effects — the extracellular LDLa domain controls desensitization while the positively charged intracellular C-tail reduces polyamine-dependent rectification without altering Ca²⁺ permeability.

    Evidence Patch-clamp electrophysiology with domain-deletion and point-mutation Neto1/Neto2 constructs in heterologous cells

    PMID:22973017

    Open questions at the time
    • How the C-terminal domain physically interferes with polyamine block was unknown
    • Contribution of CUB domains not yet dissected
  3. 2012 High

    Demonstrating that NETO2 bridges KARs to the scaffolding protein GRIP and is required for synaptic KAR retention answered how auxiliary subunits regulate receptor localization beyond gating — Neto2-null cerebellum showed ~40% PSD loss of GluK2 without change in total levels.

    Evidence Co-immunoprecipitation, PSD fractionation, and heterologous co-expression in Neto2-null mouse cerebellum

    PMID:23236500

    Open questions at the time
    • Whether NETO2–GRIP interaction is regulated by activity or phosphorylation was unknown
    • Synaptic consequences in other brain regions not yet examined
  4. 2013 High

    The discovery that NETO2 physically associates with KCC2 and maintains its oligomeric active form revealed an unexpected non-ionotropic function — linking an ion channel auxiliary subunit to chloride homeostasis and GABAergic inhibition.

    Evidence Reciprocal co-immunoprecipitation, gramicidin perforated patch-clamp, biochemical fractionation in Neto2-null hippocampal neurons

    PMID:23401525

    Open questions at the time
    • Whether NETO2 simultaneously binds KARs and KCC2 or forms separate complexes was unclear
    • Structural determinants of NETO2–KCC2 interaction not mapped
  5. 2015 High

    In vivo validation in Neto2-null mice showed depolarized EGABA, reduced KCC2 surface expression and Ser-940 phosphorylation, diminished sIPSCs, and PTZ-seizure susceptibility, establishing that NETO2–KCC2 interaction has physiological and pathological consequences at the circuit level.

    Evidence Gramicidin perforated patch-clamp, surface biotinylation, PTZ seizure induction in Neto2-null mice

    PMID:26441539

    Open questions at the time
    • Whether seizure susceptibility is solely KCC2-mediated or also involves KAR dysfunction was unresolved
    • Mechanism by which NETO2 maintains KCC2 Ser-940 phosphorylation unknown
  6. 2015 High

    Identifying the M3-S2 gating linker and D1 dimer interface as determinants of NETO2 action, and showing CUB domains confer NETO2-specific (vs NETO1) desensitization modulation, mapped the receptor-side and auxiliary-subunit-side interaction surfaces that control gating.

    Evidence Site-directed mutagenesis of KAR gating linkers and Neto1/Neto2 chimeric constructs with patch-clamp electrophysiology

    PMID:26277340 PMID:26282342

    Open questions at the time
    • Direct structural visualization of the interface was still lacking
    • Whether these determinants operate identically at heteromeric receptors was unclear
  7. 2017 High

    Identification of CaMKII/PKA-dependent Ser-409 phosphorylation on NETO2 that restricts GluK1 synaptic targeting provided the first post-translational regulatory mechanism controlling NETO2-mediated KAR trafficking.

    Evidence Mass spectrometry, in vitro kinase assays, phosphomimetic/phosphodeficient mutagenesis, surface biotinylation, synaptic fractionation

    PMID:28717010

    Open questions at the time
    • Stimulus conditions triggering Ser-409 phosphorylation in vivo not defined
    • Whether phosphorylation also affects NETO2–KCC2 interaction unknown
  8. 2017 High

    NETO2 expression in DRG neurons is developmentally regulated and re-induced by MEK/ERK signaling after nerve injury; Neto2-null DRG neurons exhibit stunted neurite outgrowth, extending NETO2 function to peripheral sensory neurons and axon regeneration.

    Evidence Whole-cell patch-clamp in DRG neurons, sciatic nerve crush model, MEK/ERK pharmacological inhibition, neurite outgrowth assay in Neto2-null mice

    PMID:28235897

    Open questions at the time
    • Whether neurite outgrowth defect is KAR-dependent or KCC2-dependent not determined
    • Role in pain signaling only indirectly implied
  9. 2019 High

    Linking NETO2-dependent KAR synaptic abundance to fear circuit function showed that Neto2-null mice have elevated cued fear and delayed extinction, with 21–37% reductions in synaptic GluK2/3 and GluK5 across amygdala, mPFC, and ventral hippocampus.

    Evidence Behavioral fear conditioning, synaptosomal fractionation, immunoblotting in Neto2-null mice

    PMID:30770891

    Open questions at the time
    • Cell-type-specific contributions (excitatory vs inhibitory neurons) not resolved
    • Whether fear phenotype reflects KAR loss, KCC2 loss, or both untested
  10. 2020 High

    NETO2 loss impairs PV interneuron maturation in amygdala (reduced PV⁺PNN⁺ cells, increased glutamatergic and decreased GABAergic transmission), providing a cellular mechanism linking NETO2 to E/I balance disruption in fear circuits.

    Evidence Immunohistochemistry, whole-cell patch-clamp in amygdala slices, spine density analysis, c-Fos immunostaining in Neto2-null mice

    PMID:32788298

    Open questions at the time
    • Whether PV interneuron immaturity is cell-autonomous or circuit-level effect unknown
    • Molecular pathway from NETO2 loss to PV/PNN reduction not identified
  11. 2021 High

    Cryo-EM structures of GluK2–NETO2 complexes at inhibited and desensitized states revealed that NETO2 crosslinks ATD and LBD faces of the receptor and its TM helix competes with the post-M4 H1 helix for an intracellular cap domain, providing the structural basis for both gating modulation and rectification control.

    Evidence Cryo-EM structure determination of GluK2–NETO2 complex at multiple functional states

    PMID:34552241

    Open questions at the time
    • Open-state structure not captured
    • Structural basis for NETO2–KCC2 interaction remains unknown
  12. 2023 Medium

    In spinal dorsal horn, GluK2–NETO2 interaction is selectively upregulated after tissue injury, linking NETO2 to postoperative pain hypersensitivity via downstream PKCγ activation and AMPAR synaptic incorporation.

    Evidence Co-immunoprecipitation, intrathecal siRNA, synaptic fractionation, pain behavioral assays in rat plantar incision model

    PMID:37544581

    Open questions at the time
    • Single-lab finding not independently replicated
    • Whether NETO2 directly activates PKCγ or via KAR-mediated Ca²⁺ influx not resolved
  13. 2023 Medium

    Identification of NETO2 CUB1 domain as the receptor for soluble LRIG3 in tumor-associated macrophages, mediating suppression of M2 polarization, extended NETO2 function outside the nervous system to immune regulation in the tumor microenvironment.

    Evidence Mass spectrometry, co-immunoprecipitation, NETO2 knockout and CUB1 domain deletion in TAMs

    PMID:36639372

    Open questions at the time
    • Single-lab observation; independent replication needed
    • Downstream signaling pathway from NETO2 in macrophages not delineated
    • Whether neuronal NETO2 functions of CUB1 and immune functions are mutually exclusive unknown
  14. 2025 High

    Time-resolved cryo-EM of apo, agonist-bound, and PAM-activated GluK2±NETO2 showed that NETO2 does not alter individual LBD or channel conformation during activation but prevents tightening of the inter-LBD-dimer interface, specifically explaining how NETO2 slows deactivation without fundamentally altering the activation gate.

    Evidence Time-resolved cryo-EM of GluK2–Neto2 in apo closed, kainate-activated open, and BPAM344-activated open states

    PMID:40846810

    Open questions at the time
    • Desensitized-to-open transition mechanism not structurally captured
    • Heteromeric receptor structures with NETO2 still lacking
  15. 2025 High

    Quantitative kinetic measurements established NETO2 as the more impactful auxiliary subunit, slowing GluK2 channel opening ~7-fold and closing ~3-fold (vs ~2-fold for NETO1), providing the biophysical basis for differential NETO1/NETO2 modulation of KAR signaling.

    Evidence Laser-pulse photolysis with microsecond resolution combined with whole-cell patch-clamp in HEK-293 cells

    PMID:41197725

    Open questions at the time
    • Whether these kinetic differences translate to distinct synaptic current waveforms in native neurons not directly tested
    • Kinetics of heteromeric receptor–NETO2 complexes not measured

Open questions

Synthesis pass · forward-looking unresolved questions
  • Key unresolved questions include whether NETO2 simultaneously engages KARs and KCC2 in a single macromolecular complex or forms separate pools, the structural basis of the NETO2–KCC2 interaction, and how cell-type-specific NETO2 expression partitions its dual roles in excitatory gating versus inhibitory chloride homeostasis.
  • No structural data on NETO2–KCC2 complex
  • No conditional knockout studies parsing KAR-dependent vs KCC2-dependent phenotypes in specific neuron types
  • Heteromeric KAR–NETO2 cryo-EM structures not yet available

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 7 GO:0060090 molecular adaptor activity 3
Localization
GO:0005886 plasma membrane 6
Pathway
R-HSA-112316 Neuronal System 12 R-HSA-162582 Signal Transduction 4 R-HSA-382551 Transport of small molecules 2
Partners
CAMK2AGRIK1GRIK2GRIK5GRIP1LRIG3SLC12A5
Complex memberships
KAR–NETO2 complex (GluK2 homomeric and GluK1/GluK5, GluK2/GluK5 heteromeric)NETO2–KCC2 complex

Evidence

Reading pass · 18 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2013 Neto2 physically associates with KCC2 (K⁺-Cl⁻ cotransporter) and is required to maintain normal KCC2 protein abundance; Neto2 specifically binds the active oligomeric form of KCC2, and loss of this interaction reduces KCC2-mediated Cl⁻ extrusion, resulting in decreased synaptic inhibition in hippocampal neurons. Co-immunoprecipitation, gramicidin perforated patch-clamp, biochemical fractionation, Neto2-null mouse neurons Proceedings of the National Academy of Sciences of the United States of America High 23401525
2015 In Neto2-null hippocampal neurons, the reversal potential for GABA (EGABA) is significantly depolarized, surface levels of KCC2 are reduced, and phosphorylation of KCC2 at serine 940 is decreased; Neto2-null mice show reduced sIPSC amplitude and frequency and are susceptible to PTZ-induced seizures. Gramicidin perforated patch-clamp, surface biotinylation, immunoblotting, Neto2-null mouse model, PTZ seizure induction Frontiers in cellular neuroscience High 26441539
2011 Neto2 acts as an accessory subunit of kainate receptors and slows desensitization and deactivation of GluK1, GluK1/GluK5, and GluK2/GluK5 heteromeric receptors; Neto2 also increases the rate of recovery from desensitization in a subunit-composition-dependent manner. Rapid glutamate application to outside-out patches from heterologous cells expressing various KAR subunit combinations with or without Neto2; whole-cell recordings from neurons The Journal of neuroscience High 21632929
2012 Neto2 reduces inward rectification of GluK2(Q) kainate receptors (caused by polyamine block) without altering Ca²⁺ permeability; this effect is mediated by the intracellular C-terminal domain of Neto2 (positively charged residues), distinct from its extracellular LDLa domain which controls desensitization modulation. Patch-clamp electrophysiology in heterologous cells, domain deletion and point-mutation constructs of Neto1/Neto2 The Journal of neuroscience High 22973017
2012 Neto2 interacts with the PDZ domain-containing scaffolding protein GRIP, and this interaction promotes/stabilizes GluK2:GRIP complexes; in Neto2-null cerebellum there is ~40% reduction in GluK2-KARs at the postsynaptic density without change in total GluK2 levels, demonstrating Neto2 controls synaptic localization of KARs. Co-immunoprecipitation, postsynaptic density fractionation, Neto2-null mouse cerebellum, co-expression in heterologous cells PloS one High 23236500
2015 The M3-S2 gating linker of GluK2 is a critical determinant of Neto2 modulation of KAR desensitization; mutations in these linkers bidirectionally alter Neto2 modulation in an agonist-specific manner and abolish Neto2 modulation of heteromeric receptor desensitization. The D1 dimer interface of the ligand-binding domain is also a site of Neto2 action, where Neto2 alters cation sensitivity in a manner that is eliminated by M3-S2 linker mutations. Site-directed mutagenesis of KAR gating linkers, patch-clamp electrophysiology in heterologous cells The Journal of physiology High 26282342
2015 The extracellular N-terminal region (including the two CUB domains) of Neto2 is largely responsible for its distinct regulatory effects on desensitization of GluK1 homomeric receptors, as demonstrated by chimeric Neto1/Neto2 subunits. Chimeric Neto1/Neto2 constructs, patch-clamp electrophysiology in HEK-293T cells Neuropharmacology High 26277340
2017 Neto2 is phosphorylated at serine 409 by CaMKII and PKA both in vitro and in heterologous cells; endogenous Neto2 Ser-409 phosphorylation is detected in brain. Phosphorylation at Ser-409 (mimicked by S409D) inhibits synaptic targeting of the KAR subunit GluK1, revealing a post-translational mechanism controlling KAR synaptic localization. Mass spectrometry identification of phosphorylation site, in vitro kinase assays with CaMKII/PKA, phosphomimetic and phosphodeficient mutagenesis, surface biotinylation, synaptic fractionation The Journal of biological chemistry High 28717010
2017 Neto2 is highly expressed in neonatal DRG neurons, assembles with and alters gating kinetics of endogenous KARs in a developmentally regulated fashion, and its expression can be upregulated in adult DRG neurons via MEK/ERK signaling and after sciatic nerve crush; Neto2⁻/⁻ adult DRG neurons exhibit stunted neurite outgrowth. Whole-cell patch-clamp in DRG neurons, Neto2-null mouse, sciatic nerve crush model, MEK/ERK pharmacological inhibition, neurite outgrowth assay The Journal of neuroscience High 28235897
2019 Neto2-null mice show higher cued fear expression and delayed extinction; KAR subunits GluK2/3 are reduced by ~21–37% and GluK5 by ~17–24% at synapses in ventral hippocampus, medial prefrontal cortex, and amygdala of Neto2⁻/⁻ mice, linking Neto2-dependent KAR synaptic abundance to fear circuit function. Behavioral fear conditioning, synaptosomal fractionation, immunoblotting, in situ hybridization in Neto2-null mice Neuropsychopharmacology High 30770891
2020 Neto2⁻/⁻ adult amygdala shows a 7.5% reduction in PV⁺PNN⁺ cells and reduced PV staining intensity (suggesting PV interneuron immaturity), increased glutamatergic and reduced GABAergic transmission, increased dendritic spine density in basal amygdala, and higher c-Fos activation after fear acquisition, collectively indicating Neto2 is required for maturation of the amygdala PV interneuron network. Immunohistochemistry, whole-cell patch-clamp in amygdala slices, spine density analysis, c-Fos immunostaining in Neto2-null mice eNeuro High 32788298
2021 Cryo-EM structures of homotetrameric GluK2 in complex with NETO2 (at inhibited and desensitized states) reveal variable stoichiometry (one or two NETO2 subunits per tetramer); NETO2 accesses only two broad faces of the receptor, crosslinking lower lobe of ATD A/C, upper lobe of LBD B/D, and lower lobe of LBD A/C, explaining gating kinetics modulation; the NETO2 transmembrane helix is proximal to the selectivity filter and competes with the amphiphilic H1 helix after M4 for interaction with an intracellular cap domain formed by M1-M2 linkers, revealing the mechanism of rectification regulation. Cryo-electron microscopy structure determination of GluK2–NETO2 complex at multiple functional states Nature High 34552241
2023 Soluble LRIG3 derived from glioma cells interacts with the CUB1 domain of NETO2 in tumor-associated macrophages (TAMs), and this interaction suppresses M2 polarization of TAMs; CUB1 deletion mutation of NETO2 or NETO2 knockout prevents sLRIG3 from inhibiting M2 polarization, demonstrating that NETO2 mediates the sLRIG3 effect on macrophage polarization. Mass spectrometry, Co-immunoprecipitation, NETO2 knockout and domain deletion mutation in TAMs Cell death & disease Medium 36639372
2023 GluK2–NETO2 (but not GluK2–NETO1) interaction is upregulated in ipsilateral dorsal horn neurons 6 h after plantar incision; intrathecal NETO2 siRNA reduces GluK2-NETO2 interaction, decreases PKCγ activation, and reduces synaptic incorporation of AMPA receptor GluR1 subunits, attenuating postoperative pain hypersensitivity. Co-immunoprecipitation, intrathecal siRNA, synaptic fractionation, von Frey and pain score assays in rat plantar incision model Neuroscience letters Medium 37544581
2023 NETO2 knockdown in melanoma cells reduces intracellular Ca²⁺ levels and expression of Ca²⁺/CaMKII pathway genes, suppressing proliferation and metastasis; pharmacological inhibition of CaMKII with KN93 blocks NETO2-induced melanoma proliferation and metastasis, placing NETO2 upstream of Ca²⁺/CaMKII/CREB signaling. siRNA knockdown/overexpression, Ca²⁺ measurement, CaMKII inhibitor (KN93), proliferation and invasion assays Frontiers of medicine Medium 36738427
2025 Cryo-EM structures of rat GluK2 KAR in apo closed, and agonist (kainate) or PAM (BPAM344)-activated open states, with and without Neto2, show that Neto2 binding does not alter individual or dimeric LBD behavior or ion channel conformation during activation, but prevents tightening of the interface between two LBD dimers, thereby slowing deactivation kinetics. Time-resolved cryo-electron microscopy of GluK2–Neto2 complex in multiple functional states Nature structural & molecular biology High 40846810
2025 NETO2 slows the channel-opening rate of GluK2 homomeric receptors ~7-fold and the channel-closing rate ~3-fold (compared to ~2-fold slowing by NETO1), and reduces EC50 more significantly than NETO1, establishing NETO2 as the more impactful auxiliary subunit on GluK2 channel-opening kinetics. Laser-pulse photolysis combined with whole-cell patch-clamp recording in HEK-293 cells expressing GluK2 with NETO1 or NETO2 The Journal of biological chemistry High 41197725
2025 α5-nAChR physically interacts with NETO2 in lung adenocarcinoma cells (confirmed by Co-IP and molecular docking); acetylcholine/nicotine upregulates NETO2, p-CaMKII, p-STAT3, and vimentin via α5-nAChR, and α5-nAChR/NETO2 signaling promotes LUAD cell proliferation, migration, and invasion. Co-immunoprecipitation, molecular docking, western blot, siRNA knockdown, cell proliferation/migration/invasion assays Cancer cell international Low 40001189

Source papers

Stage 0 corpus · 32 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2021 NETO2 promotes esophageal cancer progression by inducing proliferation and metastasis via PI3K/AKT and ERK pathway. International journal of biological sciences 65 33390848
2013 Neto2 is a KCC2 interacting protein required for neuronal Cl- regulation in hippocampal neurons. Proceedings of the National Academy of Sciences of the United States of America 64 23401525
2019 NETO2 promotes invasion and metastasis of gastric cancer cells via activation of PI3K/Akt/NF-κB/Snail axis and predicts outcome of the patients. Cell death & disease 59 30770791
2011 Neto2 modulation of kainate receptors with different subunit compositions. The Journal of neuroscience : the official journal of the Society for Neuroscience 53 21632929
2020 Exosomal lncRNA FAM225A accelerates esophageal squamous cell carcinoma progression and angiogenesis via sponging miR-206 to upregulate NETO2 and FOXP1 expression. Cancer medicine 48 33006432
2012 The auxiliary subunits Neto1 and Neto2 reduce voltage-dependent inhibition of recombinant kainate receptors. The Journal of neuroscience : the official journal of the Society for Neuroscience 47 22973017
2012 Neto2 interacts with the scaffolding protein GRIP and regulates synaptic abundance of kainate receptors. PloS one 36 23236500
2021 Kainate receptor modulation by NETO2. Nature 33 34552241
2017 Neto2 Assembles with Kainate Receptors in DRG Neurons during Development and Modulates Neurite Outgrowth in Adult Sensory Neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience 26 28235897
2015 Neto2-null mice have impaired GABAergic inhibition and are susceptible to seizures. Frontiers in cellular neuroscience 21 26441539
2015 The auxiliary subunits Neto1 and Neto2 have distinct, subunit-dependent effects at recombinant GluK1- and GluK2-containing kainate receptors. Neuropharmacology 20 26277340
2015 Identification of critical functional determinants of kainate receptor modulation by auxiliary protein Neto2. The Journal of physiology 19 26282342
2019 NETO2 promotes pancreatic cancer cell proliferation, invasion and migration via activation of the STAT3 signaling pathway. Cancer management and research 17 31239769
2019 Kainate receptor auxiliary subunit NETO2 is required for normal fear expression and extinction. Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 15 30770891
2021 Long non-coding RNA SNHG17 promotes lung adenocarcinoma progression by targeting the microRNA-193a-5p/NETO2 axis. Oncology letters 14 34671432
2017 Upregulation of NETO2 gene in colorectal cancer. BMC genetics 14 29297384
2020 NETO2 Is Deregulated in Breast, Prostate, and Colorectal Cancer and Participates in Cellular Signaling. Frontiers in genetics 13 33362854
2023 Glioma-derived LRIG3 interacts with NETO2 in tumor-associated macrophages to modulate microenvironment and suppress tumor growth. Cell death & disease 12 36639372
2017 Phosphorylation of the kainate receptor (KAR) auxiliary subunit Neto2 at serine 409 regulates synaptic targeting of the KAR subunit GluK1. The Journal of biological chemistry 10 28717010
2020 Kainate Receptor Auxiliary Subunit NETO2-Related Cued Fear Conditioning Impairments Associate with Defects in Amygdala Development and Excitability. eNeuro 9 32788298
2016 Pharmacological Modulation of GluK1 and GluK2 by NETO1, NETO2, and PSD95. Assay and drug development technologies 8 26991362
2022 TYMSOS-miR-101-3p-NETO2 axis promotes osteosarcoma progression. Molecular and cellular probes 7 36509232
2021 TTN-AS1 accelerates the growth and migration of nasopharyngeal carcinoma cells via targeting miR-876-5p/NETO2. Molecular therapy oncolytics 7 35229031
2019 Reducing NETO2 expression prevents human nasopharyngeal carcinoma (NPC) progression by suppressing metastasis and inducing apoptosis. Biochemical and biophysical research communications 7 30975469
2016 Neto2 Influences on Kainate Receptor Pharmacology and Function. Basic & clinical pharmacology & toxicology 6 26928870
2025 Activation of kainate receptor GluK2-Neto2 complex. Nature structural & molecular biology 5 40846810
2023 NETO2 promotes melanoma progression via activation of the Ca2+/CaMKII signaling pathway. Frontiers of medicine 4 36738427
2025 α5-nAChR/NETO2 contributed to chronic stress-promoted lung adenocarcinoma progression. Cancer cell international 3 40001189
2025 Targeting NETO2 suppresses cell proliferation, invasion, and migration and inactivates the STAT3/C-MYC pathway in hepatocellular carcinoma. World journal of surgical oncology 2 40158169
2023 NETO2-GluK2 interaction contributes to postoperative pain hypersensitivity through inducing PKCγ activation and synaptic incorporation of AMPA receptor GluR1 subunits in rat dorsal horn. Neuroscience letters 1 37544581
2026 The role of NETO2 in neurological disorders and cancer: From molecular function to clinical relevance. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 0 41714211
2025 A comparative study of NETO1 and NETO2 on channel-opening kinetics of GluK2 kainate receptors. The Journal of biological chemistry 0 41197725