Affinage

MAK

Serine/threonine-protein kinase MAK · UniProt P20794

Length
623 aa
Mass
70.6 kDa
Annotated
2026-06-10
84 papers in source corpus 14 papers cited in narrative 14 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

MAK (male germ cell-associated kinase) is a CDC2/CDC28-related serine/threonine protein kinase that functions as a ciliary length regulator in photoreceptors and other sensory cells (PMID:2183027, PMID:21148103). Its catalytic activation requires dual phosphorylation of a TDY activation-loop motif, with CCRK serving as the upstream activating kinase that phosphorylates the activation-loop threonine, while protein phosphatase 5 provides opposing dephosphorylation; MAK family kinases recognize an R-P-X-S/T-P consensus with a stringent Arg at the P-3 position (PMID:16954377). Within sensory cilia, MAK localizes to the connecting cilium and outer-segment axoneme/ciliary tip, where it negatively regulates axonemal length by phosphorylating substrates that govern microtubule and IFT dynamics: it phosphorylates the axonemal microtubule-associated protein RP1 to limit elongation (PMID:21148103), phosphorylates multiple sites in the tubulin-binding module of IFT-74 to reduce tubulin-binding affinity and promote tubulin unloading at the ciliary tip (PMID:35969738), and phosphorylates the microtubule plus-end tracking protein FAP256/CEP104 to control axonemal assembly (PMID:41231942). Loss of MAK produces elongated cilia, accumulation of IFT proteins (IFT88, IFT57) and acetylated tubulin, mislocalized rhodopsin, and progressive photoreceptor degeneration (PMID:21148103). MAK acts in a conserved CCRK-headed kinase axis together with the related kinase ICK, and combined loss of MAK and ICK causes loss of photoreceptor axonemes and severe retinal degeneration that is partially rescued by ICK gene delivery or FGF receptor inhibition (PMID:30955935, PMID:39293864). In humans, loss-of-function MAK mutations—including an Alu insertion disrupting the retina-specific exon-9/12 transcript isoform and kinase-inactivating missense changes—cause inherited photoreceptor degeneration, and restoring MAK expression rescues ciliary length control (PMID:21825139, PMID:21835304, PMID:34518651).

Mechanistic history

Synthesis pass · year-by-year structured walk · 13 steps
  1. 1990 Medium

    Established MAK as a distinct serine/threonine kinase, defining the molecular identity and tissue context that any later mechanism would have to explain.

    Evidence cDNA cloning, sequence analysis, and Northern blot in mouse testis

    PMID:2183027

    Open questions at the time
    • No substrates or activation mechanism identified
    • Restricted to expression profiling, no functional assay
    • Apparent germ-cell restriction later proven incomplete given retinal roles
  2. 1992 Medium

    Refined MAK expression to specific meiotic spermatocyte stages, suggesting a role tied to meiotic cell division.

    Evidence Non-radioactive in situ hybridization on mouse testis sections

    PMID:1473268

    Open questions at the time
    • Localizes mRNA but not protein function
    • No causal link to meiotic events
  3. 1993 High

    Demonstrated MAK is a functional kinase by showing it phosphorylates a 210-kDa cellular phosphoprotein on Ser/Thr in vitro and in vivo, establishing catalytic activity against a cellular substrate.

    Evidence Immunoprecipitate kinase assay, subcellular fractionation, in vivo 32Pi labeling

    PMID:8321219

    Open questions at the time
    • Identity of the 210-kDa substrate never determined
    • Cytoplasmic soluble localization not connected to a pathway
  4. 2002 High

    Knockout showed MAK is dispensable for spermatogenesis, redirecting the search for its essential physiological role away from male fertility.

    Evidence Mak-null mouse generation with fertility and histological analysis

    PMID:11971961

    Open questions at the time
    • Did not reveal where MAK is essential
    • Mild motility/litter phenotype mechanistically unexplained
  5. 2006 High

    Defined the activation mechanism of the MAK family: CCRK-dependent TDY activation-loop phosphorylation, PP5 as the opposing phosphatase, and an R-P-X-S/T-P substrate consensus, providing the biochemical framework for MAK regulation.

    Evidence In vitro kinase assays, peptide library screening, mass spectrometry, co-IP, and phosphatase assays on MAK/MRK(ICK)

    PMID:16954377

    Open questions at the time
    • Much of the detailed work was on the related MRK/ICK rather than MAK itself
    • Physiological substrates not yet identified
  6. 2010 High

    Identified ciliary length control as MAK's essential function, linking its kinase activity to RP1 phosphorylation, IFT regulation, and photoreceptor survival.

    Evidence Mak-null mouse immunohistochemistry, in vitro RP1 phosphorylation, and overexpression rescue of ciliary elongation

    PMID:21148103

    Open questions at the time
    • Direct phospho-sites on RP1 not mapped in vivo
    • Mechanism by which IFT proteins accumulate not resolved
  7. 2011 Medium

    Connected MAK to human inherited retinal degeneration, showing both disrupted retina-specific isoform expression and loss of kinase activity in patient mutations established kinase function as disease-critical.

    Evidence Human donor tissue immunohistochemistry, iPSC retinal differentiation isoform analysis, and in vitro kinase assays on patient missense mutants

    PMID:21825139 PMID:21835304

    Open questions at the time
    • In vitro kinase loss not directly tied to ciliary phenotype in same study
    • Functional consequence of isoform switch on cilia not shown
  8. 2019 High

    Placed MAK genetically downstream of CCRK in a conserved ciliary kinase cascade controlling axonemal microtubule stability, length, and branching.

    Evidence C. elegans genetic epistasis, IFT and EBP-2 live imaging, and suppressor analysis in dyf-5/MAK and dyf-18/CCRK mutants

    PMID:30955935

    Open questions at the time
    • Direct kinase substrates within the axoneme not identified in this study
    • Mechanism connecting microtubule turnover to length not molecularly resolved
  9. 2021 High

    Identified IFT-74 as a direct MAK substrate and showed phosphorylation of its tubulin-binding module reduces tubulin affinity, providing a molecular mechanism for tubulin unloading at the ciliary tip.

    Evidence In vitro phosphorylation and binding-affinity measurements with phospho-ablating/mimetic IFT-74 alleles in C. elegans

    PMID:35969738

    Open questions at the time
    • Whether the same phospho-regulation operates on mammalian IFT-74 not shown
    • Spatial control of phosphorylation at the tip not resolved
  10. 2021 Medium

    Demonstrated MAK function is necessary and sufficient for photoreceptor ciliary length control, supporting gene augmentation as a corrective strategy.

    Evidence Viral MAK gene augmentation in patient iPSC-derived photoreceptor precursors and mak-knockdown zebrafish with cilia length assays

    PMID:34518651

    Open questions at the time
    • Durability and substrate-level rescue not assessed
    • Single-lab rescue without independent replication
  11. 2022 Medium

    Identified a conserved MAK-binding motif in PCARE required for proper ciliary-tip localization, linking MAK to ciliary membrane expansion machinery.

    Evidence Domain deletion mutagenesis and ciliary localization/membrane-expansion assays with WASF3 co-expression

    PMID:35253837

    Open questions at the time
    • Direct MAK-PCARE physical interaction not demonstrated in vitro
    • Whether PCARE is a MAK substrate unknown
  12. 2024 High

    Established MAK and ICK as cooperative, partially redundant CCRK-controlled regulators of photoreceptor axonemes, defining an essential IFT-regulatory axis for photoreceptor survival.

    Evidence Conditional Mak/Ick double knockout, genetic epistasis, ICK gene delivery, and FGF receptor inhibition rescue in mice

    PMID:39293864

    Open questions at the time
    • Distinct versus shared substrates of MAK and ICK not defined
    • Mechanism of FGFR-mediated ICK regulation incomplete
  13. 2025 High

    Identified FAP256/CEP104 as a MAK substrate and placed MAK downstream of the CCRK-homologous LF complex, showing kinase activity is required for ciliary assembly.

    Evidence Chlamydomonas loss-of-function, CrMAK-GFP live imaging, in vitro kinase assay on FAP256/CEP104, kinase-dead mutants, and LF-complex epistasis

    PMID:41231942

    Open questions at the time
    • Whether mammalian MAK phosphorylates CEP104 not shown
    • How FAP256/CEP104 phosphorylation mechanistically promotes assembly not resolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • How MAK integrates its multiple ciliary substrates (RP1, IFT-74, FAP256/CEP104, PCARE) into coordinated length and assembly control, and the identity of its original germ-cell 210-kDa substrate, remain unresolved.
  • No unified model linking the distinct substrates spatially and temporally
  • Germ-cell substrate and meiotic role uncharacterized
  • Mammalian counterparts of invertebrate/algal substrate findings not directly confirmed

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 5 GO:0008092 cytoskeletal protein binding 3 GO:0016740 transferase activity 3
Localization
GO:0005929 cilium 3 GO:0005829 cytosol 1
Pathway
R-HSA-1852241 Organelle biogenesis and maintenance 4 R-HSA-1643685 Disease 3
Partners
CCRKCEP104ICKIFT74PCAREPP5RP1

Evidence

Reading pass · 14 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1990 MAK (male germ cell-associated kinase) encodes a novel serine/threonine protein kinase whose predicted product contains protein kinase consensus motifs in its amino-terminal region and is approximately 40% identical to the cdc2-CDC28 gene family. Its transcripts are expressed almost exclusively in testicular germ cells entering and after meiosis. cDNA cloning, sequence analysis, Northern blot Molecular and cellular biology Medium 2183027
1992 MAK mRNA is specifically localized in late pachytene (stage X) and diplotene (stage XI) spermatocytes, and faintly in dividing spermatocytes (stage XII) and early round spermatids, indicating expression is concentrated around meiotic cell division. Non-radioactive in situ hybridization on frozen mouse testis sections Cell biochemistry and function Medium 1473268
1993 MAK protein products (66- and 60-kDa) are most abundant in late pachytene spermatocytes, localized mostly in the cytoplasm as a soluble form, and are associated with a 210-kDa cellular phosphoprotein that MAK phosphorylates on serine and threonine residues both in vitro (immunoprecipitate kinase assay) and in vivo. Immunoprecipitation, in vitro kinase assay, subcellular fractionation, [35S]methionine labeling, 32Pi in vivo labeling Molecular and cellular biology High 8321219
2002 MAK is dispensable for spermatogenesis and male fertility in mice; Mak-null mice develop normally with intact spermatogenesis, though litter sizes and sperm motility in vitro are mildly reduced. Gene targeting to generate Mak−/− mice; fertility assays, histological analysis of spermatogenesis Molecular and cellular biology High 11971961
2006 MAK and its related kinase MRK/ICK require dual phosphorylation in a TDY activation-loop motif: an unidentified threonine kinase (CCRK for MRK) phosphorylates T157, and tyrosine autophosphorylation also occurs. CCRK is the activating T157 kinase for MRK (not CDK7/cyclin H/MAT1). Protein phosphatase 5 (PP5) interacts with MRK and dephosphorylates it at T157, acting as the opposing phosphatase. MRK preferentially phosphorylates R-P-X-S/T-P consensus sites (most stringent requirement: Arg at P-3). MRK phosphorylates the antiapoptotic protein Scythe at T1080 in vitro, validated by site-directed mutagenesis and mass spectrometry. In vitro kinase assay, combinatorial peptide library screening, site-directed mutagenesis, mass spectrometry, co-immunoprecipitation, in situ phosphatase assay Molecular and cellular biology High 16954377
2010 Mak negatively regulates ciliary length in retinal photoreceptors. Mak localizes to connecting cilia and outer-segment axonemes. In Mak-null mice, photoreceptors exhibit elongated cilia, accumulation of IFT88 and IFT57, expansion of Kif3a and acetylated α-tubulin signals in cilia, and abnormal rhodopsin accumulation in cell bodies at P14, followed by progressive degeneration. Overexpression of RP1 (a microtubule-associated protein in outer-segment axonemes) induces ciliary elongation, and co-expression of Mak rescues this elongation. The RP1 N-terminal portion is phosphorylated by Mak in vitro. Mak-null mouse generation, immunohistochemistry, immunofluorescence, in vitro kinase assay, overexpression rescue experiments Proceedings of the National Academy of Sciences of the United States of America High 21148103
2011 MAK is expressed in the inner segments, cell bodies, and axons of rod and cone photoreceptors in human donor tissue. An Alu insertion in exon 9 of MAK causes loss of the exon 9+12 retinal transcript isoform, which normally predominates in retinal precursors differentiated from iPSCs. The developmental switch from the exon-9-lacking to the exon-9/12-bearing transcript does not occur in patient-derived cells. Immunohistochemistry of human donor tissue, iPSC differentiation to retinal precursors, RT-PCR isoform analysis Proceedings of the National Academy of Sciences of the United States of America Medium 21825139
2011 Missense mutations in MAK that alter conserved kinase residues result in loss of kinase activity as demonstrated by in vitro kinase assay, establishing that kinase activity is critical for MAK function in humans. In vitro kinase assay on patient-derived missense mutant MAK proteins American journal of human genetics Medium 21835304
2019 In C. elegans, DYF-5/MAK (ortholog of mammalian MAK) acts downstream of DYF-18/CCRK in a kinase cascade to regulate ciliary length and branching morphology. Loss of dyf-5/MAK results in dramatically elongated, unbranched AWA cilia. Axonemal microtubules in dyf-18/CCRK mutants are decorated by EBP-2 end-binding protein along their lengths and show increased tubulin load and reduced tubulin turnover, indicating that CCRK-MAK activity tunes cilia length and shape by modulating axonemal microtubule stability. Genetic epistasis (double mutants), live imaging of IFT motor proteins, EBP-2 end-binding protein dynamics, microtubule-destabilizing tubulin mutations, IFT protein mutations as suppressors Current biology : CB High 30955935
2021 The ciliary kinase DYF-5/MAK phosphorylates multiple sites within the tubulin-binding module of IFT-74, reducing the tubulin-binding affinity of the IFT-74/81 module approximately sixfold. Ablation or constitutive activation of IFT-74 phosphorylation abnormally elongates or shortens sensory cilia in C. elegans, respectively, establishing that MAK-dependent phosphorylation of IFT-74 regulates ciliary tubulin unloading at the tip. In vitro phosphorylation assay, binding affinity measurements, C. elegans genetics with phospho-ablating and phospho-mimetic IFT-74 mutants, ciliary length phenotyping Proceedings of the National Academy of Sciences of the United States of America High 35969738
2021 Gene delivery of retinal MAK transgene to patient iPSC-derived photoreceptor precursor cells and mak-knockdown zebrafish restores the ability to regulate primary cilia length, confirming that MAK kinase function is necessary and sufficient for ciliary length control in photoreceptors. Viral gene augmentation in patient iPSC-derived cells, mak knockdown zebrafish model, cilia length assay, rt-PCR and western blotting for transgene expression Gene therapy Medium 34518651
2022 PCARE contains an evolutionarily conserved binding site for the photoreceptor kinase MAK (RP62). The MAK-binding motif within PCARE is required for PCARE's proper localization and function at the ciliary tip membrane expansion in photoreceptors. Domain deletion mutagenesis, ciliary localization assays, co-expression with WASF3 for membrane expansion measurement Human molecular genetics Medium 35253837
2024 MAK is a ciliary tip-localized IFT regulator that cooperatively acts with the related kinase ICK. Simultaneous disruption of Mak and Ick in mice results in loss of photoreceptor ciliary axonemes and severe retinal degeneration, a more severe phenotype than either single knockout. CCRK kinase acts as an upstream activator of both Mak and Ick in retinal photoreceptor cells. Gene delivery of Ick and pharmacological inhibition of FGF receptors (negative regulators of Ick) ameliorate retinal degeneration in Mak-null mice. Conditional double knockout (Mak/Ick), histology, gene delivery rescue, pharmacological FGF receptor inhibition, genetic epistasis Life science alliance High 39293864
2025 Chlamydomonas MAK (CrMAK), related to mammalian ICK/MAK, localizes to cilia via its C-terminal tail and is enriched at the ciliary tip during active ciliary assembly. CrMAK is constitutively active during ciliary assembly, acts downstream of the LF1/LF3/LF2 (CCRK homologue) ciliary length-regulatory complex, and phosphorylates FAP256/CEP104 (a microtubule plus-end tracking protein). Loss of CrMAK or its kinase activity leads to aciliated cells; dephosphorylation of FAP256/CEP104 impairs ciliary assembly. Genetic loss-of-function, live cell imaging of CrMAK-GFP fusion, in vitro kinase assay showing FAP256/CEP104 phosphorylation, kinase-dead mutants, dephosphorylation experiments, epistasis with LF complex Proceedings of the National Academy of Sciences of the United States of America High 41231942

Source papers

Stage 0 corpus · 84 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2011 Exome sequencing and analysis of induced pluripotent stem cells identify the cilia-related gene male germ cell-associated kinase (MAK) as a cause of retinitis pigmentosa. Proceedings of the National Academy of Sciences of the United States of America 178 21825139
2010 Negative regulation of ciliary length by ciliary male germ cell-associated kinase (Mak) is required for retinal photoreceptor survival. Proceedings of the National Academy of Sciences of the United States of America 117 21148103
1990 A novel mammalian protein kinase gene (mak) is highly expressed in testicular germ cells at and after meiosis. Molecular and cellular biology 113 2183027
2011 Exome sequencing and cis-regulatory mapping identify mutations in MAK, a gene encoding a regulator of ciliary length, as a cause of retinitis pigmentosa. American journal of human genetics 89 21835304
1987 Comparison of keratin monoclonal antibodies MAK-6, AE1:AE3, and CAM-5.2. American journal of clinical pathology 79 2443001
2006 Identification of yin-yang regulators and a phosphorylation consensus for male germ cell-associated kinase (MAK)-related kinase. Molecular and cellular biology 71 16954377
2013 HAM-2 and HAM-3 are central for the assembly of the Neurospora STRIPAK complex at the nuclear envelope and regulate nuclear accumulation of the MAP kinase MAK-1 in a MAK-2-dependent manner. Molecular microbiology 66 24028079
2012 WSC-1 and HAM-7 are MAK-1 MAP kinase pathway sensors required for cell wall integrity and hyphal fusion in Neurospora crassa. PloS one 56 22879952
1979 Mak mutants of yeast: mapping and characterization. Journal of bacteriology 55 387719
2014 Fungal communication requires the MAK-2 pathway elements STE-20 and RAS-2, the NRC-1 adapter STE-50 and the MAP kinase scaffold HAM-5. PLoS genetics 54 25411845
1993 Testis-specific mak protein kinase is expressed specifically in the meiotic phase in spermatogenesis and is associated with a 210-kilodalton cellular phosphoprotein. Molecular and cellular biology 53 8321219
2017 Extracellular TDP-43 aggregates target MAPK/MAK/MRK overlapping kinase (MOK) and trigger caspase-3/IL-18 signaling in microglia. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 44 28336525
2012 Circadian activation of the mitogen-activated protein kinase MAK-1 facilitates rhythms in clock-controlled genes in Neurospora crassa. Eukaryotic cell 41 23125351
2002 A testicular germ cell-associated serine-threonine kinase, MAK, is dispensable for sperm formation. Molecular and cellular biology 40 11971961
2011 Autosomal recessive retinitis pigmentosa caused by mutations in the MAK gene. Investigative ophthalmology & visual science 37 22110072
2019 A CCRK and a MAK Kinase Modulate Cilia Branching and Length via Regulation of Axonemal Microtubule Dynamics in Caenorhabditis elegans. Current biology : CB 36 30955935
2003 A Mak-like kinase is a repressor of GAMYB in barley aleurone. The Plant journal : for cell and molecular biology 33 12609043
1999 Generation of phagocytic MAK and MAC-DC for therapeutic use: characterization and in vitro functional properties. Experimental hematology 29 10210333
1985 Androgen regulation of MAK mRNAs in mouse kidney. The Journal of biological chemistry 25 2410417
2007 Expression of MAK-V/Hunk in renal distal tubules and its possible involvement in proliferative suppression. American journal of physiology. Renal physiology 23 17299141
2022 DYF-5/MAK-dependent phosphorylation promotes ciliary tubulin unloading. Proceedings of the National Academy of Sciences of the United States of America 21 35969738
2013 Transcriptional profiling of Neurospora crassa Δmak-2 reveals that mitogen-activated protein kinase MAK-2 participates in the phosphate signaling pathway. Fungal genetics and biology : FG & B 21 23733042
1992 In situ localization of male germ cell-associated kinase (mak) mRNA in adult mouse testis: specific expression in germ cells at stages around meiotic cell division. Cell biochemistry and function 19 1473268
2015 Hunk/Mak-v is a negative regulator of intestinal cell proliferation. BMC cancer 17 25881306
2015 Identification and Characterization of MicroRNAs in Ginkgo biloba var. epiphylla Mak. PloS one 17 25978425
2014 Retinitis pigmentosa caused by mutations in the ciliary MAK gene is relatively mild and is not associated with apparent extra-ocular features. Acta ophthalmologica 16 25385675
2013 Distinct expression patterns of ICK/MAK/MOK protein kinases in the intestine implicate functional diversity. PloS one 16 24244486
2000 The MAK-V protein kinase regulates endocytosis in mouse. Molecular & general genetics : MGG 15 11129044
2015 Efficient In Silico Identification of a Common Insertion in the MAK Gene which Causes Retinitis Pigmentosa. PloS one 12 26558903
2023 MAPK/MAK/MRK overlapping kinase (MOK) controls microglial inflammatory/type-I IFN responses via Brd4 and is involved in ALS. Proceedings of the National Academy of Sciences of the United States of America 9 37399380
2019 Classification or non-classification of substances with positive tumor findings in animal studies: Guidance by the German MAK commission. Regulatory toxicology and pharmacology : RTP 9 31433998
2016 Intraretinal cystoid spaces in a patient with retinitis pigmentosa due to mutation in the MAK gene. Ophthalmic genetics 9 26894652
2016 Involvement of MAK-1 and MAK-2 MAP kinases in cell wall integrity in Neurospora crassa. Bioscience, biotechnology, and biochemistry 9 27268441
2005 Cloning and sequence analysis of the 22-kDa antigen genes of Orientia tsutsugamushi strains Kato, TA763, AFSC 7, 18-032460, TH1814, and MAK 119. Annals of the New York Academy of Sciences 9 16481519
1980 Isolation and characterization of temperature-sensitive mak mutants of Saccharomyces cerevisiae. Journal of bacteriology 9 7002907
2007 MAK-4 and -5 supplemented diet inhibits liver carcinogenesis in mice. BMC complementary and alternative medicine 8 17559639
1993 Retinol differentially regulates male germ cell-associated kinase (mak) messenger ribonucleic acid expression during spermatogenesis. Biology of reproduction 7 8286591
2021 Development and biological characterization of a clinical gene transfer vector for the treatment of MAK-associated retinitis pigmentosa. Gene therapy 6 34518651
2021 Coordinated Regulation of Protoperithecium Development by MAP Kinases MAK-1 and MAK-2 in Neurospora crassa. Frontiers in microbiology 6 34899653
2021 The GUL-1 Protein Binds Multiple RNAs Involved in Cell Wall Remodeling and Affects the MAK-1 Pathway in Neurospora crassa. Frontiers in fungal biology 6 37744127
2004 Subcellular localization of MAK-V/Hunk protein kinase expressed in COS-1 cells. Cell biology international 6 14759768
1996 The antioxidant and antiatherogenic effects of MAK-4 in WHHL rabbits. Journal of alternative and complementary medicine (New York, N.Y.) 6 9395676
2024 Ccrk-Mak/Ick signaling is a ciliary transport regulator essential for retinal photoreceptor survival. Life science alliance 5 39293864
2023 Discovery and Characterization of MaK: A Novel Knottin Antimicrobial Peptide from Monochamus alternatus. International journal of molecular sciences 5 38139394
2017 High-resolution Imaging in Male Germ Cell-Associated Kinase (MAK)-related Retinal Degeneration. American journal of ophthalmology 5 29103961
2012 Identification of Nedd4 E3 ubiquitin ligase as a binding partner and regulator of MAK-V protein kinase. PloS one 5 22745772
1991 Therapy of recurrent high-grade gliomas with surgery, autologous mitogen-activated IL-2-stimulated (MAK) killer lymphocytes, and rIL-2: II. Correlation of survival with MAK cell tumor necrosis factor production in vitro. Lymphokine and cytokine research 5 1873360
2022 PCARE requires coiled coil, RP62 kinase-binding and EVH1 domain-binding motifs for ciliary expansion. Human molecular genetics 4 35253837
2019 Plastome of mycoheterotrophic Burmannia itoana Mak. (Burmanniaceae) exhibits extensive degradation and distinct rearrangements. PeerJ 4 31608171
2018 Slowly progressive retinitis pigmentosa caused by two novel mutations in the MAK gene. Ophthalmic genetics 4 29781741
2011 Interaction between MAK-V protein kinase and synaptopodin. Biochemistry. Biokhimiia 4 21568852
2000 The Necessity of Developing New Criteria for Carcinogen Classification of Fibers by the Mak Commission. Inhalation toxicology 4 26368644
1999 The lack of binding of methyl-n-amyl ketone (MAK) to rat liver DNA as demonstrated by direct binding measurements, and 32P-postlabeling techniques. Mutation research 4 10393282
2011 Classification of skin sensitizing substances: a comparison between approaches used by the DFG-MAK Commission and the European Union legislation. Regulatory toxicology and pharmacology : RTP 3 21798301
2008 Membrane localization of the MAK-V protein kinase. Biochemistry. Biokhimiia 3 18393762
2004 MAK-5 treatment enhances the nerve growth factor-mediated neurite outgrowth in PC12 cells. Journal of ethnopharmacology 3 15234748
2025 Ellipsoid Zone Loss as an Outcome Measure in MAK-Associated Retinitis Pigmentosa. American journal of ophthalmology 2 40578471
2025 N,N',N''-Tris(β-hydroxyethyl)hexahydro-1,3,5-triazine: MAK Value Documentation, addendum - Translation of the German version from 2023. The MAK collection for occupational health and safety 2 41586737
2022 MAPK/MAK/MRK overlapping kinase mediated apoptosis through caspase signaling pathway from Cristaria plicata. Developmental and comparative immunology 2 35460761
2022 Downregulation of mapk/mak/mrk overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus. Journal of medical biochemistry 2 36042901
2000 Characterization of a Mak subgroup Cdc2-like protein kinase from sugar beet (Beta vulgaris L.). Journal of experimental botany 2 11141185
2025 N,N',N''-Tris(β-hydroxypropyl)hexahydro-1,3,5-triazine: MAK Value Documentation - Translation of the German version from 2023. The MAK collection for occupational health and safety 1 41584500
2020 From the T-cell receptor to cancer therapy: an interview with Tak W. Mak. Cell death and differentiation 1 33335286
2017 [Survival Benefit by Combined Administration of Cyclophosphamide, Lentinula edodes Mycelia Extract(LEM), and Ganoderma lucidum Mycelia Extract(MAK)in S1018B10 Tumor-Bearing Mice]. Gan to kagaku ryoho. Cancer & chemotherapy 1 29066688
2000 [Experimental study on the influence of Gynostemma pentaphyllam Mak upon point mutation of Ha-ras oncogene in blocking leukoplakia from canceration]. Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology 1 11780494
2026 Formononetin ameliorates SP-induced urticaria in mice via suppressing TAK1/MAK signaling pathway. PloS one 0 41575991
2026 Single-cell RNA-seq reveals a key role for Vibrio cholerae Mak toxins in Tetrahymena pyriformis killing and bacterial survival. Frontiers in microbiology 0 41657991
2026 MAK-2 Kinase Is Required for Extended Longevity and Enhanced Stress Resistance Resulting From Mild Impairment of Mitochondrial Function in isp-1 Mutants. Aging cell 0 42126134
2025 Chlamydomonas protein kinase MAK phosphorylates FAP256/CEP104 and regulates axonemal microtubule assembly. Proceedings of the National Academy of Sciences of the United States of America 0 41231942
2025 Tetramethylol acetylenediurea: MAK Value Documentation - Translation of the German version from 2023. The MAK collection for occupational health and safety 0 41584501
2025 N,N-Dimethyl-p-toluidine: MAK Value Documentation - Translation of the German version from 2022. The MAK collection for occupational health and safety 0 41586081
2025 Naphthalene: MAK Value Documentation, addendum - Translation of the German version from 2022. The MAK collection for occupational health and safety 0 41586701
2025 Synthetic amorphous silica: MAK Value Documentation, addendum - Translation of the German version from 2023. The MAK collection for occupational health and safety 0 41971562
2025 Zinc diamyldithiocarbamate: MAK Value Documentation - Translation of the German version from 2023. The MAK collection for occupational health and safety 0 41971568
2025 Chloroform: MAK Value Documentation, addendum - Translation of the German version from 2022. The MAK collection for occupational health and safety 0 41973600
2024 Triphenylphosphine: MAK Value Documentation, addendum - Translation of the German version from 2022. The MAK collection for occupational health and safety 0 42164637
2024 Toluene: MAK Value Documentation, addendum - Translation of the German version from 2021. The MAK collection for occupational health and safety 0 42164639
2024 1,2-Dimethylhydrazine: MAK Value Documentation, addendum - Translation of the German version from 2021. The MAK collection for occupational health and safety 0 42164643
2024 Monomethylhydrazine: MAK Value Documentation, addendum - Translation of the German version from 2021. The MAK collection for occupational health and safety 0 42164646
2024 1,1-Dimethylhydrazine: MAK Value Documentation, addendum - Translation of the German version from 2021. The MAK collection for occupational health and safety 0 42164652
2005 [Molecular cloning and characterization of the mouse mak-v/Hunk gene promoter]. Molekuliarnaia biologiia 0 15773550
2002 [The use of the two-hybrid cloning in yeast for functional characterization of protein kinase MAK-V]. Molekuliarnaia biologiia 0 12068635
1995 Protoplast culture and plant regeneration from the suspension cells of Gynostemma pentaphyllum (Thumb) Mak. Chinese journal of biotechnology 0 8679938
1975 [Characterisation of RNA from synchronously growing yeast cells (Saccharomyces cerevisiae) as separated by columns of methylated albumin on Kieselgur (MAK) (author's transl)]. Zeitschrift fur Naturforschung. Section C, Biosciences 0 125971

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