Affinage

MAGI1

Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1 · UniProt Q96QZ7

Length
1491 aa
Mass
164.6 kDa
Annotated
2026-06-10
86 papers in source corpus 54 papers cited in narrative 54 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

MAGI1 is a multi-domain MAGUK scaffolding protein with an inverted domain architecture (N-terminal GuK, two WW domains, and multiple PDZ domains) that organizes epithelial and endothelial cell-cell junctions and assembles signaling complexes through its modular interaction surfaces (PMID:9395497). At junctions it engages the cadherin/catenin machinery—binding beta-catenin through its fifth PDZ domain to drive membrane localization and complex with E-cadherin during junction formation (PMID:10772923)—and localizes specifically to tight junctions alongside ZO-1 (PMID:10618722). Its PDZ and WW domains recruit a diverse repertoire of transmembrane and adhesion partners, including JAM4, ESAM, Dll1, nephrin, and the actin-associated proteins synaptopodin and alpha-actinin-4, linking junctional receptors to the cortical cytoskeleton (PMID:12042308, PMID:12773569, PMID:15383320, PMID:15908431, PMID:16155592). A central output of this scaffolding is activation of the small GTPase Rap1: MAGI1 binds the Rap1 GEF (PDZ-GEF1/Rap GEP) at junctions and is required for cadherin-engagement-induced Rap1 activation and junction maturation (PMID:11168587, PMID:16339077, PMID:27707879). MAGI1 also controls the surface abundance and stability of multiple membrane proteins, including ion channels and transporters (Slo1/BKCa, GLT-1, NaV1.8) and the coxsackie-adenovirus receptor, in a PDZ-domain-specific manner (PMID:19403801, PMID:21426345, PMID:30860870, PMID:22718816). Through these activities MAGI1 functions as a tumor suppressor, stabilizing E-cadherin/beta-catenin junctions, suppressing Wnt signaling, and restraining migration and invasion in colorectal, gastric, and breast cancers (PMID:21666716, PMID:28373751, PMID:33707576); a latent MAGI1-PP2A complex dephosphorylates S6K to limit protein synthesis, an activity held off by SRC phosphorylation (PMID:38748774). In endothelium MAGI1 is heavily regulated by post-translational modification, with p90RSK-mediated S741 phosphorylation and SENP2-controlled K931 deSUMOylation governing Rap1-dependent EC activation, nuclear translocation, ATF6/ER-stress responses, permeability, and Hippo (LATS/YAP) signaling under disturbed flow (PMID:30944250, PMID:33304925). MAGI1 is a prominent target of viral oncoproteins—HPV E6 (proteasomal degradation), Ad9 E4-ORF1 (cytoplasmic sequestration), and HTLV-1 Tax1 (mislocalization)—whose disruption of MAGI1 produces loss of tight junction integrity and deregulated growth (PMID:11077444, PMID:21123374, PMID:23279616, PMID:24696483), and it is cleaved by caspases-3/7 at Asp761 to release junctional contacts during apoptosis (PMID:17191119).

Mechanistic history

Synthesis pass · year-by-year structured walk · 16 steps
  1. 1997 High

    Established MAGI1 as a structurally unusual MAGUK, defining the inverted domain layout (GuK, WW, PDZ) and splice-variant-dependent nuclear versus membrane partitioning that frames all later mechanistic work.

    Evidence cDNA cloning, domain mapping, and subcellular fractionation of splice variants

    PMID:9395497

    Open questions at the time
    • No functional role assigned to individual domains at this stage
    • Nuclear function of the long isoform not mechanistically defined
  2. 1999 High

    Localized MAGI1 specifically to tight junctions with ZO-1 rather than to adherens junctions, placing it as a candidate junction organizer distinct from other MAGUKs.

    Evidence Immunofluorescence, fractionation, and Ca2+-switch assay in epithelial cells

    PMID:10618722

    Open questions at the time
    • Binding partners at tight junctions not yet identified
    • Mechanism of recruitment unresolved
  3. 2000 High

    Identified beta-catenin/E-cadherin binding via the fifth PDZ domain as the determinant of MAGI1 membrane localization, linking it directly to the cadherin junctional machinery.

    Evidence Co-IP, GFP localization, and yeast two-hybrid in MDCK cells

    PMID:10772923

    Open questions at the time
    • Did not establish a downstream signaling output
    • Relationship between PDZ5-catenin binding and tight junction localization unclear
  4. 2001 High

    Defined MAGI1 as a scaffold for a Rap1-specific GEF at junctions, establishing the first link between MAGI1 scaffolding and small-GTPase signaling.

    Evidence Cell-free binding, co-IP, with MAGUK specificity controls

    PMID:11168587

    Open questions at the time
    • Functional consequence of Rap1 GEF recruitment not yet tested
    • No loss-of-function data
  5. 2005 High

    Demonstrated that MAGI1 is functionally required for cadherin-engagement-induced Rap1 activation and adhesion maturation, converting the earlier binding observation into a causal pathway.

    Evidence siRNA knockdown, GTP-Rap1 pulldown, FRET biosensor in endothelial cells

    PMID:16339077

    Open questions at the time
    • Whether Rap1 output applies across all junction types not addressed
    • Upstream regulators of the MAGI1-GEF complex unknown
  6. 2005 High

    Expanded the partner repertoire to adhesion and slit-diaphragm proteins (nephrin, JAM4, Dll1) with domain-resolved binding, showing MAGI1 organizes multiple junctional/adhesion modules.

    Evidence Yeast two-hybrid, in vitro binding, immunoelectron microscopy, co-IP across systems

    PMID:12773569 PMID:15908431 PMID:16155592

    Open questions at the time
    • In vivo requirement for each interaction not yet established
    • Combinatorial assembly of tripartite complexes not fully resolved
  7. 2007 High

    Identified caspase-3/7 cleavage at Asp761 as the mechanism by which MAGI1 junctional anchoring is dismantled during apoptosis, linking the scaffold to programmed cell death.

    Evidence In vitro caspase cleavage, Asp761Ala mutagenesis, apoptosis assays

    PMID:17191119

    Open questions at the time
    • Physiological trigger of cleavage in tissue not defined
    • Fate and function of the resulting fragments only partially characterized [#15]
  8. 2011 High

    Established MAGI1 as a tumor suppressor that stabilizes E-cadherin/beta-catenin junctions and suppresses Wnt signaling and invasion in vivo.

    Evidence Reciprocal overexpression/knockdown, Wnt reporter, xenograft/orthotopic models in colorectal cancer

    PMID:21666716

    Open questions at the time
    • Direct biochemical mechanism of Wnt suppression not fully resolved
    • Generality across cancer types tested only later
  9. 2011 High

    Generalized MAGI1's role in membrane-protein surface regulation, showing PDZ-mediated control of ion channel and transporter surface abundance (Slo1, GLT-1).

    Evidence Yeast two-hybrid/GST pulldown, biotinylation, electrophysiology, glutamate uptake, siRNA

    PMID:19403801 PMID:21426345

    Open questions at the time
    • Trafficking machinery downstream of MAGI1 binding not defined
    • Whether retention vs internalization is the mechanism unclear
  10. 2011 High

    Resolved the structural and thermodynamic basis of MAGI1 PDZ1 binding to HPV16 E6, revealing a disorder-to-order transition and allosteric tail dynamics governing affinity.

    Evidence NMR structure, backbone dynamics, ITC, and mutagenesis

    PMID:21238461 PMID:25590897

    Open questions at the time
    • Whether the same dynamics govern endogenous ligand binding not tested
    • Functional impact of the C-terminal extension in cells unaddressed
  11. 2016 High

    Provided in vivo genetic evidence that MAGI1 supports slit-diaphragm integrity via nephrin membrane localization and contact-induced Rap1 activation, with disease relevance in compound mutants.

    Evidence Podocyte knockdown, global and compound knockout mice, Rap1 assays, Drosophila screen

    PMID:27707879

    Open questions at the time
    • Single-gene knockout is phenotypically normal, indicating redundancy not yet mapped
    • Tissue-specific requirements beyond glomerulus unaddressed
  12. 2018 High

    Defined the structural determinant of MAGI1-versus-MAGI2 specificity for nephrin, explaining paralog-selective scaffolding through an atypical PDZ recognition mode.

    Evidence MAGI1-PDZ3/nephrin-PBM crystal structure with gain-of-function mutagenesis

    PMID:30006415

    Open questions at the time
    • Whether other partners use similar atypical recognition not tested
    • In vivo consequence of disrupting the Gly(-3) contact unknown
  13. 2020 High

    Mapped MAGI1 as a hub for flow- and stress-responsive endothelial signaling controlled by p90RSK phosphorylation (S741) and SENP2-dependent deSUMOylation (K931), connecting it to EC activation, permeability, ER stress, and Hippo signaling.

    Evidence Phosphoproteomics, PTM-site mutants, SUMOylation/permeability assays, Magi1+/- mice

    PMID:30944250 PMID:31035633 PMID:33304925

    Open questions at the time
    • How nuclear MAGI1 complexes execute transcriptional effects not fully defined
    • Integration of Rap1, Hippo, and ER-stress outputs into a single circuit unresolved
  14. 2021 High

    Extended MAGI1 function to focal adhesions and RhoA/Rac1 signaling and dissected its tumor-suppressive mechanism via an AMOTL2/p38 stress pathway.

    Evidence siRNA, colocalization, GTPase activation assays, AMOTL2 knockout and p38 inhibitor rescue, in vivo angiogenesis

    PMID:33707576 PMID:33823745

    Open questions at the time
    • Direct biochemical link from MAGI1 to AMOTL2 sequestration not fully mapped
    • Reconciliation of RhoA-activating and RhoA-suppressing contexts unresolved
  15. 2024 High

    Identified a latent MAGI1-PP2A tumor-suppressive complex that dephosphorylates S6K, held inactive by SRC phosphorylation, providing a druggable mechanism in IDH-mutant cholangiocarcinoma.

    Evidence Unbiased phosphoproteomics, co-IP, PP2A activity and S6K assays, patient-derived xenografts

    PMID:38748774

    Open questions at the time
    • SRC phospho-site(s) on MAGI1 not pinpointed in the timeline
    • Whether the PP2A complex operates outside cholangiocarcinoma untested
  16. 2026 High

    Characterized the biophysical logic of the MAGI1 WW tandem, showing avidity-driven bidentate binding via conformational selection tuned by pH and inter-motif linker length.

    Evidence Time-resolved kinetics, ITC, and linker-swap binding assays

    PMID:41786191

    Open questions at the time
    • Physiological bidentate ligands engaging both WW domains not identified
    • In-cell relevance of pH-tuning not demonstrated

Open questions

Synthesis pass · forward-looking unresolved questions
  • How MAGI1's many domain-specific interactions, PTM states, and pathway outputs (Rap1, Wnt, Hippo, RhoA, PI3K/AKT, MAPK/ERK) are coordinated into context-specific programs in distinct tissues remains unresolved.
  • No unified model linking junctional, cytosolic, and nuclear MAGI1 pools
  • Stoichiometry and competition among partners for shared PDZ/WW domains undefined
  • Tissue-specific PTM regulation not systematically mapped

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 6 GO:0098772 molecular function regulator activity 5 GO:0008092 cytoskeletal protein binding 2
Localization
GO:0005886 plasma membrane 5 GO:0005856 cytoskeleton 3 GO:0005634 nucleus 2 GO:0005815 microtubule organizing center 1
Pathway
R-HSA-162582 Signal Transduction 6 R-HSA-1500931 Cell-Cell communication 5 R-HSA-1643685 Disease 4 R-HSA-168256 Immune System 2 R-HSA-5357801 Programmed Cell Death 2
Partners
ADAM17ATF6CTNNB1ESAMJAM4NPHS1PDZ-GEF1PTEN
Complex memberships
MAGI1-PP2A complexglomerular slit diaphragmtight junction

Evidence

Reading pass · 54 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 MAGI-1 is a MAGUK protein with a unique inverted domain structure: GuK domain at the N-terminus, two WW domains replacing the SH3 domain, and five PDZ domains. The longest splice variant (MAGI-1c) contains bipartite nuclear localization signals and localizes predominantly to the nucleus, while shorter forms lacking these signals localize to membrane and cytoplasmic fractions. cDNA cloning, subcellular fractionation, sequence analysis of splice variants The Journal of biological chemistry High 9395497
2000 MAGI-1b fifth PDZ domain binds beta-catenin and is essential for membrane localization; MAGI-1b forms complexes with beta-catenin and E-cadherin during formation of cell-cell junctions in MDCK cells, and GFP-MAGI-1b localizes to the basolateral membrane of polarized MDCK cells. Co-immunoprecipitation, GFP fusion localization, subcellular fractionation, yeast two-hybrid Biochemical and biophysical research communications High 10772923
1999 MAGI-1/BAP1 localizes specifically to tight junctions in intestinal epithelial cells and MDCK cells, co-localizing with ZO-1 (not E-cadherin), and is recruited with ZO-1 to tight junction-like structures upon PMA treatment after low-Ca2+ switch, distinguishing its role from SAP97/hDLG. Immunofluorescence, subcellular fractionation, Ca2+-switch assay Oncogene High 10618722
2000 Ad9 E4-ORF1 oncoprotein binds MAGI-1 and aberrantly sequesters it in the cytoplasm, while high-risk HPV E6 proteins bind MAGI-1 and target it for degradation; transformation-defective viral mutants are deficient for these activities. Co-immunoprecipitation, subcellular fractionation, viral mutant analysis Oncogene High 11077444
2000 MAGI-1/BAP1 serves as a scaffolding molecule for Rap GEP (a Rap1-specific GDP/GTP exchange factor) at tight junctions in epithelial cells; this interaction is specific to MAGI-1 and not observed with PSD-95/SAP90 or SAP97/hDLG. Cell-free binding assays, co-immunoprecipitation in intact cells, Northern blot Genes to cells : devoted to molecular & cellular mechanisms High 11168587
2001 MAGI-1 fifth PDZ domain binds megalin via the DSDV PDZ-binding motif at megalin's C-terminus; a mutant megalin lacking the terminal valine cannot bind. MAGI-1 is expressed in glomerular podocytes and associated with the cytoskeleton in glomerular preparations. Yeast two-hybrid, PDZ domain binding assays, mutagenesis, immunofluorescence, Western blot fractionation Journal of the American Society of Nephrology : JASN High 11274227
2001 The first PDZ domain of MAGI-1 interacts with mNET1, a Rho family nucleotide exchange factor, via a consensus PDZ-binding motif (PPxY-like at C-terminus of mNET1) plus a nearby cluster of basic residues required for the interaction. Yeast two-hybrid, GST pull-down, co-immunoprecipitation Biochemical and biophysical research communications Medium 11350080
2002 MAGI-1 WW domain 2 interacts with synaptopodin (an actin-bundling protein) identified via cDNA library screen; the fifth PDZ domain of MAGI-1 binds alpha-actinin-4 C-terminus. Both interactions were confirmed in vivo by co-immunoprecipitation from HEK293 cells, and all three proteins colocalize at tight junctions in MDCK cells. Yeast two-hybrid/cDNA library screen, in vitro GST pull-down, co-immunoprecipitation, immunofluorescence The Journal of biological chemistry High 12042308
2003 JAM4 (junctional adhesion molecule 4) directly binds MAGI-1 (but not ZO-1), and their co-expression in COS-7 cells induces clustering. MAGI-1 strengthens JAM4-mediated cell adhesion in L cells and sealing effects in CHO cell monolayers. MAGI-1 also recruits ZO-1, occludin to JAM4-based contacts. In vitro binding assay, co-immunoprecipitation, cell adhesion assay, permeability assay, fluorescence microscopy Molecular and cellular biology High 12773569
2004 ESAM (endothelial cell-selective adhesion molecule) directly binds MAGI-1 via PDZ domain-mediated interaction at ESAM's C-terminus; ESAM recruits MAGI-1 to cell-cell contacts in CHO cells, and in HUVECs MAGI-1 colocalizes with ESAM at endothelial cell-cell contacts. Yeast two-hybrid, GST pull-down, co-immunoprecipitation, immunofluorescence Experimental cell research High 15383320
2005 MAGI-1 is required for Rap1 activation upon VE-cadherin homophilic engagement at cell-cell contacts in endothelial cells. MAGI-1 binds PDZ-GEF1 (a Rap1 GEF) and localizes to cell-cell contacts via beta-catenin. MAGI-1 depletion suppresses VE-cadherin-dependent Rap1 activation, inhibits vinculin relocalization to cell-cell contacts, and impairs VE-cadherin-mediated adhesion. siRNA knockdown, Rap1 activation assay (pulldown of GTP-Rap1), FRET-based Rap1 biosensor, co-immunoprecipitation, immunofluorescence Molecular biology of the cell High 16339077
2005 MAGI-1 binds Dll1 (Delta-like 1) and N-cadherin/beta-catenin complexes; MAGI-1 recruits Dll1 to adherens junctions in cultured fibroblasts and stabilizes Dll1 on the cell surface, suggesting MAGI-1 presents Dll1 at junctions to activate Notch on neighboring cells. Yeast two-hybrid, co-immunoprecipitation, immunofluorescence, cell surface stability assay The Journal of biological chemistry High 15908431
2005 MAGI-1 is a component of the glomerular slit diaphragm and directly binds nephrin via its middle PDZ domains (PDZ3) through nephrin's C-terminus; MAGI-1 forms a tripartite complex with nephrin and JAM4 in vitro. In puromycin aminonucleoside nephrotic podocytes, MAGI-1 co-localizes with nephrin at the displaced slit diaphragm. Yeast two-hybrid, in vitro binding assay, immunoelectron microscopy, co-immunoprecipitation Laboratory investigation High 16155592
2005 A novel tight junction protein, MASCOT, binds the first WW domain of MAGI-1 via an LPxY motif (not the canonical PPxY); the coiled-coil domain of MASCOT is required for its localization to tight junctions in MDCK cells. CDNA library screen, in vitro GST binding assay, co-immunoprecipitation, immunofluorescence Biochimica et biophysica acta Medium 16019084
2007 MAGI-1 is cleaved by caspases-3 and -7 at Asp761 during apoptosis, generating a 97 kDa N-terminal fragment that dissociates from the membrane and a C-terminal fragment. Mutation of Asp761 to Ala abolishes caspase-induced cleavage and delays disruption of cell-cell contacts during apoptosis without affecting nuclear condensation. In vitro caspase cleavage assay, site-directed mutagenesis, cell-based apoptosis assays, caspase inhibitor (Z-VAD-fmk) Apoptosis High 17191119
2007 N-terminal MAGI-1 caspase cleavage product translocates to the cytosol while C-terminal caspase cleavage product accumulates in the nucleus; both overexpressed fragments exhibit minor pro-apoptotic activity when expressed in MDCK cells. GFP-fusion subcellular localization, immunofluorescence, apoptosis assay Biological chemistry Medium 17976012
2008 MAGI-1 PDZ4 domain binds the C-terminal PDZ-binding site of the hair-cell-specific Cdh23(+68) splice variant; MAGI-1 immunoreactivity is detectable in neonatal stereocilia in a distribution similar to Cdh23, with punctate staining maintained into adulthood. Cochlear cDNA library screen, PDZ domain binding assay, immunofluorescence The Journal of neuroscience Medium 18971469
2009 MAGI-1 binds BKCa (Slo1) channel proteins via a yeast two-hybrid interaction; co-expression of MAGI-1 with Slo1 in HEK293T cells significantly reduces Slo1 surface expression as assessed by biotinylation, confocal microscopy, and whole-cell recordings. Partial siRNA knockdown of endogenous MAGI-1 in podocytes increases surface expression of endogenous Slo1. Yeast two-hybrid, co-immunoprecipitation, GST pull-down, cell-surface biotinylation, electrophysiology, siRNA knockdown American journal of physiology. Cell physiology High 19403801
2008 MAGI-1 in C. elegans (ortholog) controls GLR-1/GLR-2 AMPA receptor synaptic localization in response to prior mechanosensory experience; MAGI-1L isoform interacts with AMPARs through the intracellular domain of GLR-2 subunit; mutations preventing GLR-1 ubiquitination prevent the decrease in AMPAR localization in magi-1 mutants. Genetic loss-of-function, fluorescence imaging of GFP-tagged GLR-1/GLR-2, behavioral assays, epistasis analysis PloS one High 19242552
2009 MAGI-1 in C. elegans controls associative learning via RIA interneurons and memory consolidation via AVA/AVD/AVE glutamatergic interneurons; during memory consolidation, MAGI-1 regulates GLR-1 (iGluR) clustering cell-autonomously in a manner dependent on its ability to interact with beta-catenin HMP-2. Genetic rescue (neuron-specific expression), behavioral assays, fluorescence imaging of GLR-1 clustering, epistasis analysis PloS one High 19551147
2010 ESAM-MAGI-1 co-localization promotes actin polymerization through PDZ domain interactions resulting in firm cell-cell adhesion; ESAM-MAGI-1 interaction activates RhoA, and RhoA inhibition blocks ESAM-mediated MAGI-1 recruitment and mature cell adhesion. Transfection experiments in CHO cells, cell dissociation assay, actin polymerization inhibitor, RhoA activity assay, RhoA inhibitor Genes to cells Medium 20298433
2010 MAGI-1 is a major degradation target of HPV-16 and HPV-18 E6 in cervical cancer cells; E6 preferentially targets MAGI-1 within the nucleus and at membrane sites; MAGI-1 degradation causes loss of tight junction integrity (ZO-1 mislocalization); E6 ablation restores tight junctions in a MAGI-1-dependent manner. siRNA E6 ablation, Western blot, immunofluorescence of ZO-1, rescue experiments Journal of virology High 21123374
2010 C. elegans MAGI-1 localizes apical to both the CCC and DAC junctional sub-compartments; loss of MAGI-1 causes loss of junctional compartmentalization along the lateral membrane and reduces robustness of cell-cell adhesion by both junctional types. RNAi knockdown, fluorescence imaging of junctional markers, genetic analysis Developmental biology High 21034729
2011 NMR solution structure of MAGI-1 PDZ1 domain alone and bound to HPV16 E6 C-terminal peptide reveals that binding induces quenching of high-frequency motions in the C-terminal tail of the PDZ domain; mutations in the C-terminal flanking region significantly decrease E6 binding affinity, indicating global PDZ response with effects propagated to distal sites. NMR structure determination, backbone dynamics analysis, site-directed mutagenesis, binding affinity measurement Journal of molecular biology High 21238461
2011 MAGI-1 overexpression in colorectal cancer cells stabilizes E-cadherin and beta-catenin at cell-cell junctions, enhances actin stress fiber and focal adhesion formation, suppresses Wnt signaling, and inhibits migration, invasion, and anchorage-independent growth. MAGI-1 silencing has opposite effects. MAGI-1 overexpression suppresses tumor growth and metastasis in vivo. Stable overexpression/siRNA knockdown, in vitro migration/invasion assays, Wnt reporter assay, in vivo xenograft/orthotopic models Oncogene High 21666716
2011 MAGI-1 directly binds glutamate transporter GLT-1 via GST pull-down confirmed by co-immunoprecipitation; co-expression of MAGI-1 reduces GLT-1 surface expression in C6 glioma cells; siRNA knockdown of endogenous MAGI-1 in astrocytes increases glutamate uptake and GLT-1 surface expression. GST pull-down, co-immunoprecipitation, cell-surface biotinylation, glutamate uptake assay, siRNA knockdown Journal of neurochemistry High 21426345
2011 MAGI-1 overexpression in hepatocellular carcinoma HepG2 cells inhibits migration and invasion; PTEN protein expression is significantly elevated in MAGI-1-overexpressing cells, with a positive correlation between MAGI-1 and PTEN protein levels. Stable transfection, wound healing assay, Matrigel invasion assay, Western blot Zhong nan da xue xue bao Medium 21685691
2012 MAGI-1 PDZ1 and PDZ3 domains regulate CAREx8 levels in opposing ways: PDZ3 reduces apical CAREx8 abundance and adenovirus infection, while PDZ1 rescues CAREx8 and adenovirus infection from MAGI-1-mediated suppression. Yeast two-hybrid, biochemical pull-down, co-immunoprecipitation, FRET, adenovirus infection assay Journal of virology High 22718816
2012 In C. elegans, MAGI-1 physically interacts with AFD-1/afadin and SAX-7/L1CAM (which binds MAGI-1 via its C-terminus); MAGI-1 and AFD-1 localize to a unique domain in the apical junction; SAX-7/L1CAM is required for normal MAGI-1 junctional accumulation; MAGI-1 depletion causes loss of spatial segregation and expansion of apical junctional domains. Genome-wide RNAi screen, co-immunoprecipitation, fluorescence localization, genetic epistasis Current biology High 22981773
2012 siRNA depletion of MAGI-1 activates IRF3 and induces the IFN-β promoter; avian influenza NS1 ESEV PBM sequesters MAGI-1 away from the plasma membrane in infected cells, and the ESEV PBM relative to EPEA shows relative deficiency in NS1 inhibition of IFN-β induction, suggesting MAGI-1 normally suppresses IFN-β signaling. siRNA knockdown, IRF3 activation assay, IFN-β promoter luciferase reporter, immunofluorescence PloS one Medium 22911767
2012 MAGI-1 in neuronal tissue is enriched in synaptosomal vesicle and synaptic plasma membrane fractions (distinct from MAGI-2/3 which are in PSD fractions); MAGI-1 shows diffuse distribution in hippocampal neuron cell body and processes, not enriched at synapses, unlike MAGI-2/3. Biochemical fractionation, immunofluorescence, immunohistochemistry Journal of neuroscience research Medium 22605569
2013 HTLV-1 Tax1 interacts with MAGI-1 in a PDZ-binding motif (PBM)-dependent manner and mislocalizes MAGI-1 from the detergent-soluble to the detergent-insoluble cellular fraction in 293T cells and HTLV-1-infected T-cells; Tax1-induced T-cell transformation selects for cells with irreversibly reduced MAGI-1 mRNA expression. Co-immunoprecipitation, subcellular fractionation, PBM mutant analysis, RT-PCR Cancer science Medium 23279616
2013 MAGI-1 forms an immunocomplex with p75NTR (low-affinity NGF receptor) and Shc adaptor in PC12 cells; MAGI-1 knockdown inhibits NGF-induced neurite outgrowth; both knockdown and overexpression of MAGI-1 suppress NGF-stimulated Shc-ERK pathway activation. Co-immunoprecipitation, siRNA knockdown, overexpression, neurite outgrowth assay, ERK activation assay Biochimica et biophysica acta Medium 23769981
2014 A K499E mutation in MAGI-1 PDZ1 domain renders it resistant to HPV E6 targeting; re-expression of this mutant MAGI-1 in HPV-positive cells increases ZO-1 and PAR3 recruitment to cell-cell contacts, represses cell proliferation, and induces apoptosis. Site-directed mutagenesis, lentiviral expression in HPV+ cells, immunofluorescence, cell proliferation and apoptosis assays Journal of virology High 24696483
2015 MAGI-1 PDZ3 domain is exclusively responsible for high-affinity interaction with the 7-exon CAR isoform; interruption of this high-affinity interaction alters MAGI-1 localization (CAR traffics MAGI-1 to cell junctions) but does not significantly alter adenovirus infection via CAR. Yeast two-hybrid, in vitro pull-down, co-immunoprecipitation, FRET, adenovirus infection assay The international journal of biochemistry & cell biology Medium 25622559
2015 Binding of E6 peptides to MAGI-1 PDZ1 is accompanied by an unusually large negative change in heat capacity attributed to a disorder-to-order transition of the PDZ1 C-terminal extension; NMR relaxation data confirm this; a PDZ1 mutant abolishing this transition shows different thermodynamic signature. Isothermal titration calorimetry (ITC), NMR 15N relaxation, site-directed mutagenesis Biochemistry High 25590897
2016 MAGI-1 depletion in cultured podocytes reduces nephrin and neph1 membrane localization and weakens tight junction integrity; global magi1 knockout mice are normal, but combined MAGI-1 knockout with nephrin heterozygosity causes spontaneous glomerulosclerosis; MAGI-1 depletion reduces intercellular contact-induced Rap1 activation, and combined overexpression of MAGI-1 with nephrin increases Rap1 activation requiring the nephrin-binding interface. siRNA knockdown, global knockout mice, compound genetic mouse models, Rap1 activation assay, Drosophila genetic screen, Western blot The Journal of biological chemistry High 27707879
2017 MAGI-1 knockdown in gastric cancer cells promotes migration and invasion by increasing MMP expression and EMT-related molecules via activation of the MAPK/ERK signaling pathway. shRNA knockdown, migration/invasion assays, Western blot for MMPs and EMT markers, ERK signaling analysis Chinese journal of cancer research Medium 28373751
2018 Crystal/biochemical structure analysis shows nephrin C-terminal PBM specifically binds MAGI1 PDZ3 but not MAGI2 PDZ3; the Gly at the -3 position of nephrin-PBM is the determining feature for MAGI1-PDZ3 recognition (contrasting typical PDZ/PBM binding mode); a single gain-of-function mutation in MAGI2 enables nephrin-PBM binding. Complex crystal structure (MAGI1-PDZ3/nephrin-PBM), biophysical binding assays, site-directed mutagenesis Journal of the American Society of Nephrology : JASN High 30006415
2019 MAGI1 is required for fluid shear stress-induced eNOS phosphorylation and NO production in endothelial cells; MAGI1 silencing impairs KLF4 expression and cell alignment under flow; MAGI1 overexpression induces phosphorylation of PKA, AMPK, and CaMKII; PKA and AMPK inhibition prevents MAGI1-mediated eNOS phosphorylation; endothelial-specific transgenic MAGI1 increases PKA and eNOS phosphorylation in vivo. siRNA silencing, overexpression, NO measurement, kinase phosphorylation assays, pharmacological kinase inhibitors, transgenic mice Cells High 31035633
2019 In endothelial cells exposed to disturbed flow, p90RSK binds MAGI1 and causes MAGI1-S741 phosphorylation, which upregulates EC activation via Rap1; MAGI1-K931 deSUMOylation (mediated by SENP2) induces nuclear translocation of p90RSK-MAGI1 and ATF6-MAGI1 complexes promoting EC activation and apoptosis respectively; MAGI1 associates with ATF-6 (ER stress mediator); reduced MAGI1 in Magi1-/+ mice inhibits disturbed flow-induced atherogenesis. Co-immunoprecipitation, phosphoproteomic analysis, phospho-site mutagenesis, SUMOylation assay, Magi1+/- mouse model, microarray JCI insight High 30944250
2019 Magi-1 directly interacts with NaV1.8 channels and Slack KNa channels in dorsal root ganglion neurons; Magi-1 regulates NaV1.8 plasma membrane localization, retention, and stability; DRG-specific knockdown of Magi-1 attenuates thermal nociception and inflammatory pain; a competing cell-penetrating peptide mimetic from NaV1.8 WW binding motif decreases sodium currents and NaV1.8 surface expression. co-immunoprecipitation, cell-surface biotinylation, siRNA knockdown, electrophysiology, in vivo pain behavior, peptide competition assay FASEB journal High 30860870
2020 p90RSK-mediated MAGI1 post-translational modifications (S741 phosphorylation and K931 de-SUMOylation) regulate endothelial permeability; dominant negative p90RSK or MAGI1-S741A decreases thrombin-induced permeability; p90RSK overexpression, MAGI1 siRNA, or MAGI1-K931R SUMOylation mutant accelerates permeability; MAGI1 depletion increases LATS1/2 expression, inhibiting YAP/TAZ (Hippo pathway). ECIS-based permeability assay, siRNA knockdown, PTM mutant overexpression, LATS/YAP expression analysis, in vivo p90RSK inhibitor Frontiers in cardiovascular medicine High 33304925
2021 MAGI1 colocalizes with paxillin, β3-integrin, talin 1, tensin 3, and α4-actinin at mature focal adhesions and actin stress fibers in endothelial cells; MAGI1 silencing reduces focal adhesion formation and maturation, cell spreading, actin stress fiber formation, and RhoA/Rac1 activation, and increases paxillin Y118 phosphorylation (indicator of focal adhesion turnover). MAGI1 silencing reduces integrin-dependent adhesion, increases invasion, promotes tubulogenesis in vitro, and promotes angiogenesis in vivo. siRNA silencing, immunofluorescence colocalization, RhoA/Rac1 activation assay, paxillin phosphorylation Western blot, cell adhesion/invasion assays, in vivo angiogenesis model Cell adhesion & migration High 33823745
2021 Loss of MAGI1 in breast cancer cells causes accumulation of E-cadherin and AMOTL2 with increased cellular stiffness, elevated ROCK and p38 stress kinase activities (but low YAP activity); MAGI1 loss-driven tumorigenicity is rescued by AMOTL2 deletion or p38 inhibition, demonstrating MAGI1 suppresses tumorigenesis by inhibiting an AMOTL2/p38 stress pathway. MAGI1 siRNA knockdown, AMOTL2 knockout, p38 inhibitor, ROCK inhibitor, tumorigenicity assays, YAP activity measurement Scientific reports High 33707576
2021 MAGI-1 PDZ2 domain blockade by decoy peptides (TAT-E6, TAT-NET1) decreases CAREx8 expression and adenovirus transduction; this occurs via enhanced regulated intramembrane proteolysis through ADAM17 and γ-secretase; ADAM17 interacts directly with MAGI-1 PDZ3 domain; blocking PDZ2 enhances ADAM17 accessibility to CAREx8. Decoy peptide competition, cell-surface biotinylation, adenovirus transduction assay, co-immunoprecipitation of ADAM17/MAGI-1, in vivo transgenic model Journal of virology High 33762416
2022 MAGI1 inhibits IRF3 activation in endothelial cells by maintaining IRF3 SUMOylation; MAGI1 depletion leads to IRF3 nuclear translocation without phosphorylation, upregulates STAT1, IFNβ1, MX1, OAS2, and activates STAT5 upon IAV infection; MAGI1 overexpression inhibits Ifnb1 mRNA and MX1 expression. siRNA knockdown, overexpression, microarray, RT-PCR, Western blot for STAT1/MX1/OAS2, IRF3 nuclear translocation assay, IRF3 SUMOylation assay Frontiers in cardiovascular medicine Medium 36082118
2024 SRC phosphorylates MAGI1 in IDH-mutant cholangiocarcinoma; SRC inhibition (dasatinib) enables formation of a MAGI1-PP2A complex that dephosphorylates and inhibits S6K, reducing protein synthesis and causing cell death; SRC normally inhibits this latent tumor-suppressing MAGI1-PP2A function. Unbiased phosphoproteomic screen, co-immunoprecipitation, PP2A activity assay, S6K phosphorylation Western blot, cell viability assays, patient-derived xenografts Science translational medicine High 38748774
2024 Tissue factor (TF) associates predominantly with MAGI1 (less with MAGI2/3); TF interacts with the PDZ1 domain of MAGI1; phosphorylation of Ser253 in TF prevents its association with MAGI1; PAR2 activation disrupts TF-MAGI1 association; blocking PDZ1 with competing peptide augments TF procoagulant and signaling activity, suggesting MAGI1 stabilizes and 'encrypts' TF. Proximity ligation assay, co-immunoprecipitation (reciprocal), pull-down with TF cytoplasmic domain peptides, PDZ domain overexpression, thrombin generation assay Thrombosis journal High 38233821
2025 MAGI1 suppresses osteoclast fusion and differentiation through the RhoA/ROCK1 signaling pathway; MAGI1 overexpression decreases RhoA, ROCK1, and p-p65 in RANKL-treated osteoclasts; knockdown of MAGI1 in subchondral bone increases osteoclast numbers and worsens subchondral bone microarchitecture; RhoA activator narciclasine rescues MAGI1 overexpression effects. AAV-mediated shMagi1 knockdown in vivo, overexpression experiments, RANKL-induced osteoclastogenesis in vitro, LC-MS/MS, Western blot, micro-CT, histological staining Journal of orthopaedic translation Medium 40322041
2026 The MAGI-1 WW tandem architecture substantially enhances affinity towards bidentate ligands compared to individual WW domains; binding proceeds through a conformational selection mechanism sampling alternative conformational states; affinity is modulated by environmental pH with cooperative dissociation linked to electrostatic contacts; swapping linker length between PY motifs alters complex stability (longer linkers weaken binding). Time-resolved kinetic analyses, isothermal titration calorimetry, biochemical binding assays International journal of biological macromolecules High 41786191
2019 miR-486-5p directly binds the 3'-UTR of MAGI1 mRNA (confirmed by dual-luciferase and FREMSA); MAGI1 knockdown reverses hydroquinone-induced inhibition of erythroid differentiation in K562 cells via downregulation of RAPGEF2 and RAP1A (downstream of MAGI1 in Rap1 signaling). Dual-luciferase reporter assay, FREMSA, siRNA knockdown, overexpression, erythroid differentiation assay Toxicology in vitro Medium 32198055
2024 In C. elegans, the SAX-7/L1CAM PDZ-binding motif binds MAGI-1 in pull-down assays; MAGI-1 bridges SAX-7 to HMP-2/β-catenin; MAGI-1 acts in glia (non-cell-autonomously) to promote dendrite extension; MAGI-1 also binds AFD-1/afadin via SAX-7 PB motif, and loss of AFD-1 enhances sax-7 dendrite defects. Pull-down assay, cell-specific rescue, genetic depletion, double mutant analysis bioRxiv (preprint)preprint Medium 38260503
2026 OGT mediates O-GlcNAcylation of MAGI1, stabilizing its expression; OGT knockdown reduces MAGI1 levels and inhibits PI3K/AKT pathway activation in high-glucose-treated VSMCs; MAGI1 overexpression activates PI3K/AKT signaling and promotes VSMC proliferation, migration, and inflammation. OGT knockdown, MAGI1 overexpression/knockdown, O-GlcNAcylation assay, Western blot for PI3K/AKT, cell function assays, diabetic mouse model Hereditas Medium 41546072

Source papers

Stage 0 corpus · 86 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2000 Interactions of the PDZ-protein MAGI-1 with adenovirus E4-ORF1 and high-risk papillomavirus E6 oncoproteins. Oncogene 244 11077444
1997 MAGI-1, a membrane-associated guanylate kinase with a unique arrangement of protein-protein interaction domains. The Journal of biological chemistry 171 9395497
2000 MAGI-1 interacts with beta-catenin and is associated with cell-cell adhesion structures. Biochemical and biophysical research communications 146 10772923
2003 JAM4, a junctional cell adhesion molecule interacting with a tight junction protein, MAGI-1. Molecular and cellular biology 142 12773569
2005 MAGI-1 is required for Rap1 activation upon cell-cell contact and for enhancement of vascular endothelial cadherin-mediated cell adhesion. Molecular biology of the cell 128 16339077
2002 Interaction of two actin-binding proteins, synaptopodin and alpha-actinin-4, with the tight junction protein MAGI-1. The Journal of biological chemistry 116 12042308
1999 Localization of membrane-associated guanylate kinase (MAGI)-1/BAI-associated protein (BAP) 1 at tight junctions of epithelial cells. Oncogene 106 10618722
2002 MAGI-1: a widely expressed, alternatively spliced tight junction protein. Experimental cell research 82 11969287
2010 A systematic analysis of human papillomavirus (HPV) E6 PDZ substrates identifies MAGI-1 as a major target of HPV type 16 (HPV-16) and HPV-18 whose loss accompanies disruption of tight junctions. Journal of virology 74 21123374
2004 Endothelial adhesion molecule ESAM binds directly to the multidomain adaptor MAGI-1 and recruits it to cell contacts. Experimental cell research 72 15383320
2001 The membrane-associated guanylate kinase protein MAGI-1 binds megalin and is present in glomerular podocytes. Journal of the American Society of Nephrology : JASN 60 11274227
2005 MAGI1 recruits Dll1 to cadherin-based adherens junctions and stabilizes it on the cell surface. The Journal of biological chemistry 58 15908431
2000 Membrane-associated guanylate kinase with inverted orientation (MAGI)-1/brain angiogenesis inhibitor 1-associated protein (BAP1) as a scaffolding molecule for Rap small G protein GDP/GTP exchange protein at tight junctions. Genes to cells : devoted to molecular & cellular mechanisms 58 11168587
2009 Neuron-specific regulation of associative learning and memory by MAGI-1 in C. elegans. PloS one 57 19551147
2008 TRIP6, a novel molecular partner of the MAGI-1 scaffolding molecule, promotes invasiveness. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 57 19017743
2005 MAGI-1 is a component of the glomerular slit diaphragm that is tightly associated with nephrin. Laboratory investigation; a journal of technical methods and pathology 55 16155592
2011 Identification of MAGI1 as a tumor-suppressor protein induced by cyclooxygenase-2 inhibitors in colorectal cancer cells. Oncogene 52 21666716
2019 MAGI1 Mediates eNOS Activation and NO Production in Endothelial Cells in Response to Fluid Shear Stress. Cells 48 31035633
2019 MAGI1 as a link between endothelial activation and ER stress drives atherosclerosis. JCI insight 45 30944250
2008 MAGI-1, a candidate stereociliary scaffolding protein, associates with the tip-link component cadherin 23. The Journal of neuroscience : the official journal of the Society for Neuroscience 45 18971469
2011 The structural and dynamic response of MAGI-1 PDZ1 with noncanonical domain boundaries to the binding of human papillomavirus E6. Journal of molecular biology 44 21238461
2001 Identification of mNET1 as a candidate ligand for the first PDZ domain of MAGI-1. Biochemical and biophysical research communications 43 11350080
2014 Restoration of MAGI-1 expression in human papillomavirus-positive tumor cells induces cell growth arrest and apoptosis. Journal of virology 42 24696483
2012 MAGI1 copy number variation in bipolar affective disorder and schizophrenia. Biological psychiatry 40 22381734
2012 A genome-wide functional screen shows MAGI-1 is an L1CAM-dependent stabilizer of apical junctions in C. elegans. Current biology : CB 40 22981773
2009 MAGI-1 modulates AMPA receptor synaptic localization and behavioral plasticity in response to prior experience. PloS one 39 19242552
2005 Identification and characterization of a novel tight junction-associated family of proteins that interacts with a WW domain of MAGI-1. Biochimica et biophysica acta 38 16019084
2009 MAGI-1 interacts with Slo1 channel proteins and suppresses Slo1 expression on the cell surface. American journal of physiology. Cell physiology 36 19403801
2010 Surface plasmon resonance analysis of the binding of high-risk mucosal HPV E6 oncoproteins to the PDZ1 domain of the tight junction protein MAGI-1. Journal of molecular recognition : JMR 34 20842623
2017 MAGI1 inhibits migration and invasion via blocking MAPK/ERK signaling pathway in gastric cancer. Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 33 28373751
2014 Regulation and involvement in cancer and pathological conditions of MAGI1, a tight junction protein. Anticancer research 33 24982328
2019 Long noncoding RNA MAGI1-IT1 regulates cardiac hypertrophy by modulating miR-302e/DKK1/Wnt/beta-catenin signaling pathway. Journal of cellular physiology 32 31222747
2021 MAGI1, a Scaffold Protein with Tumor Suppressive and Vascular Functions. Cells 26 34198584
2019 Long noncoding RNA MAGI1-IT1 promoted invasion and metastasis of epithelial ovarian cancer via the miR-200a/ZEB axis. Cell cycle (Georgetown, Tex.) 25 31122127
2013 Human T-cell leukemia virus type 1 Tax protein interacts with and mislocalizes the PDZ domain protein MAGI-1. Cancer science 24 23279616
2007 Cleavage of MAGI-1, a tight junction PDZ protein, by caspases is an important step for cell-cell detachment in apoptosis. Apoptosis : an international journal on programmed cell death 24 17191119
2010 Interaction of endothelial cell-selective adhesion molecule and MAGI-1 promotes mature cell-cell adhesion via activation of RhoA. Genes to cells : devoted to molecular & cellular mechanisms 23 20298433
2016 MAGI-1 Interacts with Nephrin to Maintain Slit Diaphragm Structure through Enhanced Rap1 Activation in Podocytes. The Journal of biological chemistry 21 27707879
2015 A deletion at ADAMTS9-MAGI1 locus is associated with psoriatic arthritis risk. Annals of the rheumatic diseases 21 25990289
2012 The PDZ1 and PDZ3 domains of MAGI-1 regulate the eight-exon isoform of the coxsackievirus and adenovirus receptor. Journal of virology 21 22718816
2011 Regulation of glutamate transporter GLT-1 by MAGI-1. Journal of neurochemistry 20 21426345
2012 Biochemical and morphological characterization of MAGI-1 in neuronal tissue. Journal of neuroscience research 19 22605569
2021 MAGI1 localizes to mature focal adhesion and modulates endothelial cell adhesion, migration and angiogenesis. Cell adhesion & migration 18 33823745
2020 MAGI1, a New Potential Tumor Suppressor Gene in Estrogen Receptor Positive Breast Cancer. Cancers 18 31963297
2019 Magi-1 scaffolds NaV1.8 and Slack KNa channels in dorsal root ganglion neurons regulating excitability and pain. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 18 30860870
2012 Regulation of interferon-β by MAGI-1 and its interaction with influenza A virus NS1 protein with ESEV PBM. PloS one 18 22911767
2010 The C. elegans MAGI-1 protein is a novel component of cell junctions that is required for junctional compartmentalization. Developmental biology 18 21034729
2022 Molecular insights into the interaction of HPV-16 E6 variants against MAGI-1 PDZ1 domain. Scientific reports 17 35115618
2020 MiR-486-5p-directed MAGI1/Rap1/RASSF5 signaling pathway contributes to hydroquinone-induced inhibition of erythroid differentiation in K562 cells. Toxicology in vitro : an international journal published in association with BIBRA 16 32198055
2008 Defining the minimal interacting regions of the tight junction protein MAGI-1 and HPV16 E6 oncoprotein for solution structure studies. Protein expression and purification 16 18467125
2024 SRC inhibition enables formation of a growth suppressive MAGI1-PP2A complex in isocitrate dehydrogenase-mutant cholangiocarcinoma. Science translational medicine 15 38748774
2021 Long Non-coding RNA TMEM220-AS1 Suppressed Hepatocellular Carcinoma by Regulating the miR-484/MAGI1 Axis as a Competing Endogenous RNA. Frontiers in cell and developmental biology 15 34422806
2019 Graphene oxide disrupts the protein-protein interaction between Neuroligin/NLG-1 and DLG-1 or MAGI-1 in nematode Caenorhabditis elegans. The Science of the total environment 15 31627046
2020 MAGI1-IT1 stimulates proliferation in non-small cell lung cancer by upregulating AKT1 as a ceRNA. European review for medical and pharmacological sciences 14 32016970
2011 MAGI1 inhibits cancer cell migration and invasion of hepatocellular carcinoma via regulating PTEN. Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences 14 21685691
2024 Lactate promoted cisplatin resistance in NSCLC by modulating the m6A modification-mediated FOXO3/MAGI1-IT1/miR-664b-3p/IL-6R axis. Neoplasia (New York, N.Y.) 13 38184887
2019 MAGI1 mediates tumor metastasis through c-Myb/miR-520h/MAGI1 signaling pathway in renal cell carcinoma. Apoptosis : an international journal on programmed cell death 13 31352641
2019 Silencing Of MAGI1 Promotes The Proliferation And Inhibits Apoptosis Of Glioma Cells Via The Wnt/β-Catenin And PTEN/AKT Signaling Pathways. OncoTargets and therapy 13 32009799
2017 MAGI-1 expression is decreased in several types of human T-cell leukemia cell lines, including adult T-cell leukemia. International journal of hematology 12 29043551
2015 The PDZ3 domain of the cellular scaffolding protein MAGI-1 interacts with the Coxsackievirus and adenovirus receptor (CAR). The international journal of biochemistry & cell biology 12 25622559
2021 MAGI1 inhibits the AMOTL2/p38 stress pathway and prevents luminal breast tumorigenesis. Scientific reports 11 33707576
2020 p90RSK-MAGI1 Module Controls Endothelial Permeability by Post-translational Modifications of MAGI1 and Hippo Pathway. Frontiers in cardiovascular medicine 10 33304925
2007 Cellular localization of MAGI-1 caspase cleavage products and their role in apoptosis. Biological chemistry 10 17976012
2019 MAGI1 Inhibits the Proliferation, Migration and Invasion of Glioma Cells. OncoTargets and therapy 9 31908493
2018 Structural Basis of Highly Specific Interaction between Nephrin and MAGI1 in Slit Diaphragm Assembly and Signaling. Journal of the American Society of Nephrology : JASN 9 30006415
2015 Disorder-to-order transition of MAGI-1 PDZ1 C-terminal extension upon peptide binding: thermodynamic and dynamic insights. Biochemistry 9 25590897
2025 MAGI1 attenuates osteoarthritis by regulating osteoclast fusion in subchondral bone through the RhoA-ROCK1 signaling pathway. Journal of orthopaedic translation 7 40322041
2013 MAGI-1 acts as a scaffolding molecule for NGF receptor-mediated signaling pathway. Biochimica et biophysica acta 7 23769981
2022 The magic of MAGI-1: A scaffolding protein with multi signalosomes and functional plasticity. Biology of the cell 6 35389514
2021 MAGI-1 PDZ2 Domain Blockade Averts Adenovirus Infection via Enhanced Proteolysis of the Apical Coxsackievirus and Adenovirus Receptor. Journal of virology 6 33762416
2024 A Potential Role for MAGI-1 in the Bi-Directional Relationship Between Major Depressive Disorder and Cardiovascular Disease. Current atherosclerosis reports 5 38958925
2022 Stimulated expression of ELR+ chemokines, VEGFA and TNF-AIP3 promote mycobacterial dissemination in extrapulmonary tuberculosis patients and Cavia porcellus model of tuberculosis. Tuberculosis (Edinburgh, Scotland) 5 35763913
2022 MAGI1 inhibits interferon signaling to promote influenza A infection. Frontiers in cardiovascular medicine 5 36082118
2017 Molecular cloning, alternative splicing and mRNA expression analysis of MAGI1 and its correlation with laying performance in geese. British poultry science 4 28067534
2024 MiR-205-5p-Mediated MAGI1 Inhibition Attenuates the Injury Induced by Diabetic Nephropathy. Pharmacology 3 38325349
2021 The Long Noncoding RNA MAGI1-IT1 Regulates the miR-302d-3p/IGF1 Axis to Control Gastric Cancer Cell Proliferation. Cancer management and research 3 33833579
2019 Intron polymorphisms of MAGI-1 and ACSF2 and effects on their expression in different goose breeds. Gene 3 30902784
2024 SAX-7/L1CAM acts with the adherens junction proteins MAGI-1, HMR-1/Cadherin, and AFD-1/Afadin to promote glial-mediated dendrite extension. bioRxiv : the preprint server for biology 2 38260503
2023 MAGI1 Prevents Senescence and Promotes the DNA Damage Response in ER+ Breast Cancer. Cells 2 37566008
2021 Corrigendum: p90RSK-MAGI1 Module Controls Endothelial Permeability by Post-translational Modifications of MAGI1 and Hippo Pathway. Frontiers in cardiovascular medicine 2 33681312
2025 Quantitative proteomic analysis unveils a critical role of VARS1 in hepatocellular carcinoma aggressiveness through the modulation of MAGI1 expression. Molecular cancer 1 39810176
2024 Regulation of tissue factor activity by interaction with the first PDZ domain of MAGI1. Thrombosis journal 1 38233821
2026 OGT-mediated O-GlcNAcylation of MAGI1 exacerbates high glucose-triggered inflammation and dedifferentiation of vascular smooth muscle cells by activating the PI3K/AKT pathway. Hereditas 0 41546072
2026 Conformational selection and linker-dependent specificity in the MAGI-1 WW tandem. International journal of biological macromolecules 0 41786191
2026 Human MAGI1 expression in endothelial cells protects from the development of localized and systemic scleroderma in mice. Arthritis research & therapy 0 41814414
2026 Adjacent domains drive the emergence of misfolded intermediates in the folding pathway of the PDZ4 from MAGI1. BBA advances 0 41852832

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