Affinage

Showing PLOD1 — LH1 is a alias.

PLOD1

Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 · UniProt Q02809

Length
727 aa
Mass
83.5 kDa
Annotated
2026-06-10
100 papers in source corpus 17 papers cited in narrative 17 extracted findings
Cross-family judge vs UniProt: tie faithfulness: 6/6 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

PLOD1 (lysyl hydroxylase 1) is an endoplasmic reticulum dioxygenase (EC 1.14.11.4) that hydroxylates lysine residues in peptide linkages to form hydroxylysine in collagens, a modification essential for intermolecular crosslinking and fibril stabilization of type I and III collagens (PMID:1577494, PMID:34646388). Loss-of-function mutations distributed throughout the gene — including frameshift, nonsense, and missense variants and a recurrent exons 10-16 duplication driven by Alu-mediated recombination in introns 9 and 16 — reduce enzyme activity below 25% of normal and cause kyphoscoliotic Ehlers-Danlos syndrome (EDS type VI/kEDS) (PMID:7713497, PMID:10874315, PMID:15979919). In mice, LH1 deficiency impairs aortic collagen crosslinking and promotes angiotensin II-induced dissecting abdominal aortic aneurysm through upregulation of thrombospondin-1, MMP activation, and VSMC apoptosis, a phenotype reversed by AAV-mediated LH1 restoration (PMID:34646388). Beyond lysyl hydroxylation, PLOD1 possesses an N-terminal glucosyltransferase domain that processes UDP-glycan donor substrates, and a missense variant in this domain segregates with familial thoracic aortic aneurysm/dissection while attenuating procollagen gene upregulation in vascular smooth muscle cells (PMID:34400365). PLOD1 also acts on non-collagen substrates: it binds and stabilizes Septin2 through its hydroxylase domain to promote actin polymerization and confined cancer cell migration (PMID:36721170), and it hydroxylates the Epstein-Barr virus EBNA1 protein at K460/K461 to stabilize EBNA1 and sustain oriP-dependent viral episome replication (PMID:37262099). Across multiple tumor contexts PLOD1 is upregulated and promotes proliferation, stemness, and metabolic reprogramming via NF-κB, Hippo-YAP, and related signaling, and its abundance is controlled post-translationally and transcriptionally by regulators including lnc-URIDS, miR-34c, SIRT6, and gluconic acid-driven autophagic degradation (PMID:36721170, PMID:32801140, PMID:33420370, PMID:32446383, PMID:37936548, PMID:40339550, PMID:40290518).

Mechanistic history

Synthesis pass · year-by-year structured walk · 8 steps
  1. 1992 High

    Establishing the molecular identity of human lysyl hydroxylase defined the enzyme responsible for collagen hydroxylysine formation and located the gene for disease mapping.

    Evidence cDNA cloning and sequencing from human placenta with enzymatic annotation and chromosomal mapping to 1p36

    PMID:1577494

    Open questions at the time
    • No structural model of the catalytic domain
    • Substrate specificity beyond collagen not addressed at this stage
  2. 1994 High

    Determining the 19-exon gene architecture explained why a recurrent multi-exon duplication arises and links it mechanistically to EDS type VI.

    Evidence Genomic clone isolation, intron sequencing, and Alu repeat mapping in patient DNA

    PMID:7713497

    Open questions at the time
    • Frequency and penetrance of the duplication across populations not quantified
    • Functional consequence at the protein level not directly measured here
  3. 2000 High

    Coupling diverse loss-of-function mutations to direct enzyme activity measurements in patient fibroblasts established PLOD1 deficiency as the biochemical basis of EDS type VI.

    Evidence Full-length cDNA/genomic sequencing and enzymatic activity assays in patient dermal fibroblasts, plus splicing analysis

    PMID:10874315

    Open questions at the time
    • Genotype-phenotype correlation across mutation classes not resolved
    • Tissue-specific consequences beyond skin not assessed
  4. 2005 High

    Systematic mutation screening confirmed that varied loss-of-function alleles throughout PLOD1 converge on lysyl hydroxylase deficiency, validating molecular diagnostic strategy.

    Evidence Combined cDNA and genomic DNA sequencing in nine index EDS VIA patients

    PMID:15979919

    Open questions at the time
    • No biochemical characterization of individual novel missense variants
    • Modifier effects not addressed
  5. 2021 High

    An in vivo aneurysm model demonstrated that LH1-dependent aortic collagen crosslinking protects against dissecting aneurysm and identified thrombospondin-1 as a downstream effector amenable to rescue.

    Evidence LH1-deficient mouse with Ang II infusion, transcriptomics, AAV rescue, and thrombospondin-1 inhibition

    PMID:34646388

    Open questions at the time
    • Direct link between collagen crosslink defect and thrombospondin-1 induction not mechanistically resolved
    • Relevance to human aneurysm subtypes not established
  6. 2021 Medium

    Discovery of an N-terminal glucosyltransferase activity expanded PLOD1's enzymatic repertoire and tied a folding-destabilizing variant in this domain to familial thoracic aortic disease.

    Evidence In vitro UDP-glycan processing assay, comparison with PLOD3, and VSMC overexpression/knockdown

    PMID:34400365

    Open questions at the time
    • Glucosyltransferase activity demonstrated in vitro, not yet shown on physiological substrates in cells
    • Single lab, limited replication
    • Causality of the variant in the family not genetically proven beyond segregation
  7. 2023 Medium

    Identification of Septin2 as a stabilized binding partner revealed a non-collagen, cytoskeletal role for PLOD1 in driving confined migration and metastasis.

    Evidence Reciprocal Co-IP/LC-MS, FRET, FRAP, confined migration and 3D invasion assays, and orthotopic mouse model

    PMID:36721170

    Open questions at the time
    • Whether SEPT2 is hydroxylated or only bound is not fully resolved
    • Single lab
    • Mechanism by which binding promotes actin polymerization not detailed
  8. 2023 High

    Demonstrating PLOD1-mediated hydroxylation of EBV EBNA1 established a viral substrate and a host enzyme required for episome maintenance.

    Evidence Co-IP/MS, MS identification of hydroxylated EBNA1 peptides, K460/K461 mutagenesis, shRNA depletion, and oriP replication assays

    PMID:37262099

    Open questions at the time
    • Whether PLOD1 inhibition is therapeutically tractable against EBV in vivo unknown
    • Structural basis of EBNA1 recognition not determined

Open questions

Synthesis pass · forward-looking unresolved questions
  • How PLOD1's distinct activities — lysyl hydroxylation, glucosyltransfer, and non-collagen substrate binding/stabilization — are mechanistically partitioned across its domains, and which oncogenic signaling links are direct versus correlative, remain unresolved.
  • No structural model linking catalytic and glucosyltransferase domains to distinct substrates
  • Tumor signaling links (NF-κB, Hippo-YAP, SOX9/PI3K) are largely pathway-level without direct biochemical demonstration
  • Physiological scope of non-collagen substrates beyond SEPT2 and EBNA1 unknown

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0016491 oxidoreductase activity 4 GO:0016740 transferase activity 1 GO:0016787 hydrolase activity 1 GO:0140096 catalytic activity, acting on a protein 1
Localization
GO:0005783 endoplasmic reticulum 1
Pathway
R-HSA-1474244 Extracellular matrix organization 3 R-HSA-1643685 Disease 3 R-HSA-392499 Metabolism of proteins 2
Partners
EBNA1LNC-URIDSSEPT2

Evidence

Reading pass · 17 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1992 Human lysyl hydroxylase (PLOD1) was cloned and sequenced; it encodes a 709 amino acid polypeptide (plus 18 aa signal peptide) that catalyzes hydroxylation of lysine residues in peptide linkages (EC 1.14.11.4), forming hydroxylysine in collagens. The C-terminal region (especially residues 639-715) is 99% identical between human and chick, suggesting functionally significant sequences in this region. The gene was mapped to chromosome 1p36.2-1p36.3. cDNA cloning from human placenta lambda gt11 library, sequence analysis, Southern blot of somatic cell hybrids, in situ hybridization Genomics High 1577494
1994 The human PLOD1 gene contains 19 exons; introns 9 and 16 contain multiple Alu sequences generating extensive homology and potential recombination sites, explaining the common multi-exon duplication (exons 10-16) mutation found in Ehlers-Danlos syndrome type VI patients. The gene's 5' flanking region has housekeeping gene characteristics consistent with constitutive expression. Genomic clone isolation, intron sequencing, Alu repeat mapping, analysis of patient genomic DNA Genomics High 7713497
2000 Loss-of-function mutations in PLOD1 (including frameshift 1702insC and nonsense mutations Y142X, Q327X, R670X, Y511X, and a common 7-exon duplication) cause deficiency of lysyl hydroxylase 1 activity (<25% of normal) in Ehlers-Danlos syndrome type VI patients. Alternative splicing pathways that bypass premature termination codons were identified. Full-length cDNA amplification and sequencing from patient dermal fibroblasts, genomic DNA verification, enzymatic activity assay Human mutation High 10874315
2005 A systematic mutation analysis strategy for PLOD1 using cDNA and/or gDNA identified multiple disease-causing mutations in 9 index EDS VIA patients including novel frameshift (p.Ile454IlefsX2), missense (p.Ala667Thr), and nonsense (p.His706Arg) mutations, as well as the common exons 10-16 duplication, confirming that diverse loss-of-function mutations throughout PLOD1 cause lysyl hydroxylase deficiency. cDNA and genomic DNA sequencing, mutation analysis strategy combining both substrates Molecular genetics and metabolism High 15979919
2021 LH1 (PLOD1) deficiency in mice promotes angiotensin II-induced dissecting abdominal aortic aneurysm. LH1 deficiency upregulates thrombospondin-1, which drives proinflammatory processes, MMP activation, and VSMC apoptosis. Restoration of LH1 via AAV injection normalized thrombospondin-1 levels and alleviated AAA formation. LH1 is essential for type I/III collagen intermolecular crosslinking and stabilization in the aorta. LH1-deficient mouse model (C57Bl/6), Ang II osmotic pump infusion, whole-transcriptomic analysis, AAV rescue, pharmacologic thrombospondin-1 inhibition (TAX2), histology, MMP activity assay Theranostics High 34646388
2021 A PLOD1 missense variant p.(Ser178Arg) affecting the N-terminal glycosyltransferase domain was identified in familial thoracic aortic aneurysm/dissection. In vitro assays demonstrated that wild-type PLOD1 processes UDP-glycan donor substrates (glucosyltransferase activity), representing a previously unrecognized enzymatic function. The variant affects folding stability of the glycosyltransferase domain and attenuates procollagen gene upregulation in VSMCs overexpressing wild-type PLOD1. si-PLOD1 cells showed hypercontractility and upregulation of contractile markers. In vitro UDP-glycan processing assay, comparison with PLOD3 homolog, VSMC overexpression/knockdown, enzymatic activity measurement, collagen fibril analysis Translational research : the journal of laboratory and clinical medicine Medium 34400365
2023 PLOD1 (LH1) promotes confined migration and metastasis of cancer cells by binding and stabilizing Septin2 (SEPT2), thereby facilitating actin polymerization. This function depends on the hydroxylase domain of LH1. LH1 bound SEPT2 as identified by immunoprecipitation followed by LC-MS. Immunoprecipitation/LC-MS identification of LH1-binding proteins, FRET, FRAP, immunofluorescence, microfluidic confined migration assay, 3D invasion assay, orthotopic mouse model Molecular cancer Medium 36721170
2023 PLOD1 interacts with Epstein-Barr virus EBNA1 protein and hydroxylates EBNA1 at lysine K460 and/or K461. Mutation of K460 (but not K461) abrogates EBNA1-driven oriP DNA replication without affecting DNA binding. Mutations in both K460 and K461 perturb interactions with PLOD1 and decrease EBNA1 protein stability. PLOD1 depletion by shRNA causes loss of EBNA1 protein levels, loss of EBV episomes, and inhibition of oriP-dependent DNA replication. Proteomic analysis (Co-IP/MS), shRNA depletion, small molecule inhibition, mass spectrometry identification of hydroxylated EBNA1 peptides, site-directed mutagenesis of K460/K461, oriP replication assay PLoS pathogens High 37262099
2020 lnc-URIDS (a lncRNA) interacts with PLOD1 protein and reduces its protein stability, leading to decreased collagen production and deposition and delayed wound healing in diabetes. PLOD1 is identified as a critical enzyme responsible for collagen cross-linking in dermal fibroblasts, and its protein stability is regulated post-translationally by lnc-URIDS binding. lncRNA-protein interaction assay, knockdown in vitro and in vivo diabetic wound healing model, protein stability analysis Diabetes Medium 32801140
2021 PLOD1 knockdown in glioblastoma stem cells inhibits tumor viability, proliferation, migration, and mesenchymal transition, while promoting apoptosis. PLOD1 expression is enhanced in hypoxic environments, and its tumor-promoting effects are associated with activation of the NF-κB signaling pathway as revealed by bioinformatics and molecular experiments. shRNA knockdown in patient-derived glioma stem cells, tumor xenograft, NF-κB pathway reporter assays, hypoxia experiments Oncogene Medium 33420370
2020 PLOD1 inactivates the Hippo-YAP pathway in osteosarcoma by inhibiting phosphorylation of LATS1 (p-LATS1) and YAP (p-YAP), leading to increased nuclear YAP distribution. PLOD1 is itself a direct target of miR-34c, which inhibits PLOD1 expression by binding its 3'-UTR and suppresses OS cell growth. Gain/loss-of-function assays, immunofluorescence for YAP nuclear distribution, luciferase 3'-UTR reporter assay for miR-34c/PLOD1, in vivo xenograft Biochemical and biophysical research communications Medium 32446383
2021 PLOD1 promotes cell growth and aerobic glycolysis in gastric cancer by activating the SOX9/PI3K/Akt/mTOR signaling pathway; PLOD1 overexpression increases SOX9, p-Akt/Akt and p-mTOR/mTOR levels, and SOX9 overexpression rescues PLOD1 knockdown-mediated inhibition of cell viability and glycolysis. MTT, EDU, colony formation assays; glycolysis metabolic assays (glucose uptake, lactate, ATP, OCR, ECAR); Western blot for pathway proteins; rescue experiments with SOX9 overexpression; in vivo xenograft Frontiers in bioscience (Landmark edition) Low 34455762
2023 SIRT6 deficiency in mice reduces Plod1 expression at both mRNA and protein levels in ovarian granulosa cells, leading to decreased collagen crosslinking and deposition in the ovarian stroma and impaired follicular development. This places Plod1 downstream of SIRT6 in ovarian collagen homeostasis. SIRT6 knockout mouse model, RNA and protein expression analysis in ovaries and granulosa cells, collagen histology Aging cell Medium 37936548
2025 Gluconic acid (GLA) directly binds PLOD1 protein and promotes its autophagy-lysosomal degradation, reducing p-AKT signaling and activating autophagy to attenuate hypertrophic scar formation. PLOD1 degradation was confirmed by pull-down, cellular thermal shift assay, co-localization studies, and autophagy inhibitor experiments. Molecular docking, pull-down assay, cellular thermal shift assay (CETSA), co-localization, autophagic flux assay, transmission electron microscopy, Western blot, rabbit ear scar model Phytomedicine Medium 40339550
2025 PLOD1 promotes proliferation and stemness of hepatocellular carcinoma cells by positively regulating the NF-κB/IL-6/STAT3 signaling pathway and accelerating TCA cycle metabolic reprogramming. Blocking NF-κB/IL-6/STAT3 or TCA cycle mitigates PLOD1-induced proliferation/stemness. AAV-mediated hepatic PLOD1 knockdown reduces HCC progression in mice. RNA-seq, untargeted metabolomics, CCK8, flow cytometry, sphere formation, ALDH activity, subcutaneous/orthotopic/hepatotoxin-induced mouse models, pathway inhibitor experiments JHEP reports Medium 40290518
2019 Transcriptome profiling of patient-derived PLOD1-kEDS skin fibroblasts revealed differential expression of extracellular matrix component genes, genes involved in ER stress, protein trafficking, inner ear development, and vascular remodeling compared to controls, establishing distinct molecular features specific to PLOD1-kEDS versus FKBP14-kEDS. RNA sequencing of patient-derived skin fibroblasts, differential gene expression analysis Genes Low 31288483
2025 PCL microspheres increase collagen synthesis in fibroblasts through upregulation of SAMD11, which in turn increases PLOD1 expression. RNA-seq identified SAMD11 as a key regulator upstream of PLOD1 in PCL-stimulated collagen production; SAMD11 knockdown attenuated PLOD1 upregulation and collagen synthesis. RNA-seq of differentially expressed genes, SAMD11 knockdown, Western blot, collagen synthesis measurement, in vivo mouse skin thickness assay Biochimica et biophysica acta. Molecular cell research Low 40074205

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2014 Structure of the LH1-RC complex from Thermochromatium tepidum at 3.0 Å. Nature 150 24670637
1992 Mutants of Rhodobacter sphaeroides lacking one or more pigment-protein complexes and complementation with reaction-centre, LH1, and LH2 genes. Molecular microbiology 131 1588816
1992 Cloning of human lysyl hydroxylase: complete cDNA-derived amino acid sequence and assignment of the gene (PLOD) to chromosome 1p36.3----p36.2. Genomics 120 1577494
2013 Three-dimensional structure of the Rhodobacter sphaeroides RC-LH1-PufX complex: dimerization and quinone channels promoted by PufX. Biochemistry 94 24131108
1999 The reaction center-LH1 antenna complex of Rhodobacter sphaeroides contains one PufX molecule which is involved in dimerization of this complex. Biochemistry 94 10346905
1998 The LH1-RC core complex of Rhodobacter sphaeroides: interaction between components, time-dependent assembly, and topology of the PufX protein. Biochimica et biophysica acta 60 9814844
2021 Structures of Rhodopseudomonas palustris RC-LH1 complexes with open or closed quinone channels. Science advances 57 33523887
2021 Hypoxia-induced PLOD1 overexpression contributes to the malignant phenotype of glioblastoma via NF-κB signaling. Oncogene 52 33420370
2005 Mutation analysis of the PLOD1 gene: an efficient multistep approach to the molecular diagnosis of the kyphoscoliotic type of Ehlers-Danlos syndrome (EDS VIA). Molecular genetics and metabolism 51 15979919
1996 Consequences for the organization of reaction center-light harvesting antenna 1 (LH1) core complexes of Rhodobacter sphaeroides arising from deletion of amino acid residues from the C terminus of the LH1 alpha polypeptide. The Journal of biological chemistry 51 8621732
2012 Separation, purification, and α-glucosidase inhibition of polysaccharides from Coriolus versicolor LH1 mycelia. Carbohydrate polymers 49 23218298
2009 Structural model and excitonic properties of the dimeric RC-LH1-PufX complex from Rhodobacter sphaeroides. Chemical physics 49 20161332
2020 Novel Long Noncoding RNA lnc-URIDS Delays Diabetic Wound Healing by Targeting Plod1. Diabetes 47 32801140
2021 Cryo-EM structure of the monomeric Rhodobacter sphaeroides RC-LH1 core complex at 2.5 Å. The Biochemical journal 46 34590677
1994 Structure and expression of the human lysyl hydroxylase gene (PLOD): introns 9 and 16 contain Alu sequences at the sites of recombination in Ehlers-Danlos syndrome type VI patients. Genomics 46 7713497
1995 Genetic and biochemical characterization of the Lactobacillus delbrueckii subsp. lactis bacteriophage LL-H lysin. Applied and environmental microbiology 41 8526515
2018 Biodegradation of phenanthrene by biodemulsifier-producing strain Achromobacter sp. LH-1 and the study on its metabolisms and fermentation kinetics. Ecotoxicology and environmental safety 37 30055385
2011 Bioconversion of ginsenoside rb1 into compound k by Leuconostoc citreum LH1 isolated from kimchi. Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 35 24031746
2021 Cryo-EM structure of the dimeric Rhodobacter sphaeroides RC-LH1 core complex at 2.9 Å: the structural basis for dimerisation. The Biochemical journal 34 34622934
2024 LncRNA FOXD2-AS1 promotes the growth, invasion and migration of OSCC cells by regulating the MiR-185-5p/PLOD1/Akt/mTOR pathway. Cancer genetics 32 38729078
2021 PLOD1 promotes cell growth and aerobic glycolysis by regulating the SOX9/PI3K/Akt/mTOR signaling pathway in gastric cancer. Frontiers in bioscience (Landmark edition) 32 34455762
2018 High Expression of PLOD1 Drives Tumorigenesis and Affects Clinical Outcome in Gastrointestinal Carcinoma. Genetic testing and molecular biomarkers 32 29723071
1995 A group I intron in the terminase gene of Lactobacillus delbrueckii subsp. lactis phage LL-H. Microbiology (Reading, England) 32 7496530
2022 Asymmetric structure of the native Rhodobacter sphaeroides dimeric LH1-RC complex. Nature communications 31 35393413
2021 A previously unrecognized membrane protein in the Rhodobacter sphaeroides LH1-RC photocomplex. Nature communications 31 34728609
2002 A conserved C-terminal region in Gp71 of the small isometric-head phage LL-H and ORF474 of the prolate-head phage JCL1032 is implicated in specificity of adsorption of phage to its host, Lactobacillus delbrueckii. Journal of bacteriology 30 11948159
1986 Physical and genetic characterization of the genome of Lactobacillus lactis bacteriophage LL-H. Journal of virology 29 3016319
2015 The origin of the unusual Qy red shift in LH1-RC complexes from purple bacteria Thermochromatium tepidum as revealed by Stark absorption spectroscopy. Biochimica et biophysica acta 26 26341015
2011 Carotenoids are essential for normal levels of dimerisation of the RC-LH1-PufX core complex of Rhodobacter sphaeroides: characterisation of R-26 as a crtB (phytoene synthase) mutant. Biochimica et biophysica acta 26 21651888
2017 Association of ECRG4 with PLK1, CDK4, PLOD1 and PLOD2 in esophageal squamous cell carcinoma. American journal of translational research 25 28861165
2013 Structural studies of the cell wall polysaccharides from three strains of Lactobacillus helveticus with different autolytic properties: DPC4571, BROI, and LH1. Carbohydrate research 25 23831635
2013 The extracellular phage-host interactions involved in the bacteriophage LL-H infection of Lactobacillus delbrueckii ssp. lactis ATCC 15808. Frontiers in microbiology 25 24400001
2011 Cross-species investigation of the functions of the Rhodobacter PufX polypeptide and the composition of the RC-LH1 core complex. Biochimica et biophysica acta 25 22079525
1994 Characterization of the genome region encoding structural proteins of Lactobacillus delbrueckii subsp. lactis bacteriophage LL-H. Gene 25 7828907
2021 Lysyl hydroxylase 1 (LH1) deficiency promotes angiotensin II (Ang II)-induced dissecting abdominal aortic aneurysm. Theranostics 24 34646388
2011 Monomeric RC-LH1 core complexes retard LH2 assembly and intracytoplasmic membrane formation in PufX-minus mutants of Rhodobacter sphaeroides. Biochimica et biophysica acta 24 21663730
2023 Lysyl hydroxylase LH1 promotes confined migration and metastasis of cancer cells by stabilizing Septin2 to enhance actin network. Molecular cancer 23 36721170
2012 Potential antidiabetic activity of extracellular polysaccharides in submerged fermentation culture of Coriolus versicolor LH1. Carbohydrate polymers 22 24751027
1996 Defective site-specific integration elements are present in the genome of virulent bacteriophage LL-H of Lactobacillus delbrueckii. Applied and environmental microbiology 21 8633887
1996 The early gene region completes the nucleotide sequence of Lactobacillus delbrueckii subsp. lactis phage LL-H. Gene 21 8917075
2023 Rhodobacter capsulatus forms a compact crescent-shaped LH1-RC photocomplex. Nature communications 20 36792596
2021 New mechanistic insights to PLOD1-mediated human vascular disease. Translational research : the journal of laboratory and clinical medicine 20 34400365
2008 Partial site-specific assignment of a uniformly (13)C, (15)N enriched membrane protein, light-harvesting complex 1 (LH1), by solid state NMR. Biochimica et biophysica acta 20 18267103
2005 Characterization of a highly purified, fully active, crystallizable RC-LH1-PufX core complex from Rhodobacter sphaeroides. Photosynthesis research 20 16172926
2019 SiMPLOD, a Structure-Integrated Database of Collagen Lysyl Hydroxylase (LH/PLOD) Enzyme Variants. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 19 30721533
2003 A reaction center-light-harvesting 1 complex (RC-LH1) from a Rhodospirillum rubrum mutant with altered esterifying pigments: characterization by optical spectroscopy and cryo-electron microscopy. The Journal of biological chemistry 19 12719425
2020 Distribution of the Warmblood Fragile Foal Syndrome Type 1 Mutation (PLOD1 c.2032G>A) in Different Horse Breeds from Europe and the United States. Genes 18 33353040
2019 Transcriptome Profiling of Primary Skin Fibroblasts Reveal Distinct Molecular Features Between PLOD1- and FKBP14-Kyphoscoliotic Ehlers-Danlos Syndrome. Genes 17 31288483
2014 Aberrant assembly complexes of the reaction center light-harvesting 1 PufX (RC-LH1-PufX) core complex of Rhodobacter sphaeroides imaged by atomic force microscopy. The Journal of biological chemistry 17 25193660
1994 Probing the structure of the core light-harvesting complex (LH1) of Rhodopseudomonas viridis by dissociation and reconstitution methodology. Photosynthesis research 17 24309943
2020 PLOD1, a target of miR-34c, contributes to cell growth and metastasis via repressing LATS1 phosphorylation and inactivating Hippo pathway in osteosarcoma. Biochemical and biophysical research communications 16 32446383
2019 Warmblood fragile foal syndrome type 1 mutation (PLOD1 c.2032G>A) is not associated with catastrophic breakdown and has a low allele frequency in the Thoroughbred breed. Equine veterinary journal 15 31502696
2014 A case of Ehlers-Danlos syndrome type VIA with a novel PLOD1 gene mutation. Pediatric neurology 15 25266621
2006 Association of PLOD1 polymorphisms with bone mineral density in a population-based study of women from the UK. Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA 15 16758144
2017 Engineering of a calcium-ion binding site into the RC-LH1-PufX complex of Rhodobacter sphaeroides to enable ion-dependent spectral red-shifting. Biochimica et biophysica acta. Bioenergetics 14 28826909
2013 Single-molecule spectroscopy on RC-LH1 complexes of Rhodopseudomonas acidophila strain 10050. The journal of physical chemistry. B 14 23445346
2001 Time-dependent changes in the carotenoid composition and preferential binding of spirilloxanthin to the reaction center and anhydrorhodovibrin to the LH1 antenna complex in Rhodobium marinum. Photochemistry and photobiology 14 11594059
2000 Mutational analysis of the lysyl hydroxylase 1 gene (PLOD) in six unrelated patients with Ehlers-Danlos syndrome type VI: prenatal exclusion of this disorder in one family. Human mutation 14 10874315
2012 Ca(2+)-binding reduces conformational flexibility of RC-LH1 core complex from thermophile Thermochromatium tepidum. Photosynthesis research 13 22367594
2012 Structure of the dimeric RC-LH1-PufX complex from Rhodobaca bogoriensis investigated by electron microscopy. Philosophical transactions of the Royal Society of London. Series B, Biological sciences 13 23148268
2020 Hanoverian F/W-line contributes to segregation of Warmblood fragile foal syndrome type 1 variant PLOD1:c.2032G>A in Warmblood horses. Equine veterinary journal 12 32323341
2006 Effective plasmid pX3 transduction in Lactobacillus delbrueckii by bacteriophage LL-H. Plasmid 12 16458963
1992 Purification and crystallization of the light harvesting LH1 complex from Rhodobacter sphaeroides. Journal of molecular biology 12 1474590
2023 Regulation of EBNA1 protein stability and DNA replication activity by PLOD1 lysine hydroxylase. PLoS pathogens 11 37262099
2023 Identification of Regulatory Molecular "Hot Spots" for LH/PLOD Collagen Glycosyltransferase Activity. International journal of molecular sciences 11 37446392
2022 First reported case of fragile foal syndrome type 1 in the Thoroughbred caused by PLOD1 c.2032G>A. Equine veterinary journal 10 34939209
2020 LH1-RC light-harvesting photocycle under realistic light-matter conditions. The Journal of chemical physics 10 32321270
2007 Probing binding site of bacteriochlorophyll a and carotenoid in the reconstituted LH1 complex from Rhodospirillum rubrum S1 by Stark spectroscopy. Photosynthesis research 10 17912603
2000 Exchanging cofactors in the core antennae from purple bacteria: structure and properties of Zn-bacteriopheophytin-containing LH1. Biochemistry 10 10653655
1994 Biochemical and spectroscopic characterization of the reaction center-LH1 complex and the carotenoid-containing B820 subunit of Chromatium purpuratum. Biochimica et biophysica acta 10 8167136
1987 Transmembrane cation movements during infection of Lactobacillus lactis by bacteriophage LL-H. The Journal of general virology 10 3585280
2023 SIRT6 deficiency causes ovarian hypoplasia by affecting Plod1-related collagen formation. Aging cell 9 37936548
2021 PLOD1 acts as a tumor promoter in glioma via activation of the HSF1 signaling pathway. Molecular and cellular biochemistry 9 34845571
2011 A DNA pooling-based case-control study of myopia candidate genes COL11A1, COL18A1, FBN1, and PLOD1 in a Chinese population. Molecular vision 9 21527992
2020 The first case report of Kyphoscoliotic Ehlers-Danlos syndrome of chinese origin with a novel PLOD1 gene mutation. BMC medical genetics 8 33129265
2025 A Native LH1-RC-HiPIP Supercomplex from an Extremophilic Phototroph. Communications biology 7 39799244
2021 Posttranscriptional control of PLOD1 in adipose-derived stem cells regulates scar formation through altering macrophage polarization. Annals of translational medicine 7 34790779
1982 Factors affecting in vitro DNA ejection of the Lactobacillus lactis bacteriophage LL-H. The Journal of general virology 7 7069401
2025 PLOD1 promotes the malignancy of hepatocellular carcinoma by facilitating the NF-κB/IL-6/STAT3-dependent TCA cycle. JHEP reports : innovation in hepatology 6 40290518
2020 Mechanism of the biodemulsifier-enhanced biodegradation of phenanthrene by Achromobacter sp. LH-1. Colloids and surfaces. B, Biointerfaces 6 32673989
2018 Novel features of LH1-RC from Thermochromatium tepidum revealed from its atomic resolution structure. The FEBS journal 6 30328658
2024 PLOD1 promote proliferation and migration with glycolysis via the Wnt/β-catenin pathway in THCA. Genomics 5 39424162
2023 Hysteretic Pressure Dependence of Ca2+ Binding in LH1 Bacterial Membrane Chromoproteins. The journal of physical chemistry. B 5 36608327
2020 Rare Cases of PLOD1-Related Kyphoscoliotic Ehlers-Danlos Syndrome in a Korean Family Identified by Next Generation Sequencing. Journal of Korean medical science 5 32174067
2007 [Purification and properties of an antimicrobial substance from marine Brevibacillus laterosporus Lh-1]. Wei sheng wu xue bao = Acta microbiologica Sinica 5 18271253
1983 Effect of cadmium on the infection of Lactobacillus lactis by bacteriophage LL-H. The Journal of general virology 5 6864195
2025 Increasing collagen synthesis in fibroblasts: The roles of PCL microspheres and the SAMD11-PLOD1 axis in skin rejuvenation. Biochimica et biophysica acta. Molecular cell research 4 40074205
2025 Cryo-EM Analysis of a Tri-Heme Cytochrome-Associated RC-LH1 Complex from the Marine Photoheterotrophic Bacterium Dinoroseobacter Shibae. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 4 40112203
2023 A severe case of PLOD1-related kyphoscoliotic Ehlers-Danlos syndrome associated with several arterial and venous complications: A case report. Clinical case reports 4 36860721
2022 Modulation of blood pressure regulatory genes in the Agtrap-Plod1 locus associated with a deletion in Clcn6. Physiological reports 4 35927940
2022 CD73+ adipose-derived stem cells reduce scar formation through PLOD1. Annals of translational medicine 3 35282129
2021 Comprehensive analysis of PLOD family members in low-grade gliomas using bioinformatics methods. PloS one 3 33503035
2005 Isolation and characterisation of a Lactobacillus helveticus ITG LH1 peptidase-rich sub-proteome. International journal of food microbiology 3 16099524
2025 A novel tRNA-Derived fragment, tRF-20-M0NK5Y93 inhibits the malignant progression of non-small cell lung cancer by mediating PLOD1. Archives of biochemistry and biophysics 2 40262693
2025 Gluconic acid alleviates hypertrophic scar formation through binding PLOD1, reducing p-AKT signaling and activating autophagy. Phytomedicine : international journal of phytotherapy and phytopharmacology 2 40339550
2023 Biodegradation of benzo[a]pyrene by a marine Chlorella vulgaris LH-1 with heterotrophic ability. Marine pollution bulletin 2 38029673
2022 Case Report: Identification and Functional Analysis of a Homozygous Synonymous Variant in the PLOD1 Gene in a Chinese Neonatal With the Ehlers-Danlos Syndrome. Frontiers in pediatrics 2 35252061
2022 Prevalence of the RAPGEF5 c.2624C>A and PLOD1 c.2032G>A variants associated with equine familial isolated hypoparathyroidism and fragile foal syndrome in the US Thoroughbred population (1988-2019). Equine veterinary journal 2 36199159
2017 Carotenoid to bacteriochlorophyll energy transfer in the RC-LH1-PufX complex from Rhodobacter sphaeroides containing the extended conjugation keto-carotenoid diketospirilloxanthin. Photosynthesis research 2 28528494
2015 Ground-State Electronic Structure of RC-LH1 and LH2 Pigment Assemblies of Purple Bacteria via the EBF-MO Method. The journal of physical chemistry. A 2 26215074

Missed literature

Know a paper Affinage missed for PLOD1? Flag it for the maintainers and the community.

No submissions yet.