Affinage

KCNIP3

Calsenilin · UniProt Q9Y2W7

Length
256 aa
Mass
29.2 kDa
Annotated
2026-04-28
100 papers in source corpus 17 papers cited in narrative 17 extracted findings

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

KCNIP3 (also known as DREAM/calsenilin/KChIP3) is a multifunctional neuronal calcium sensor that acts as a Ca²⁺-regulated molecular switch coupling intracellular calcium levels to transcriptional repression, ion channel assembly, pain processing, and apoptosis. In the Ca²⁺-free state, KChIP3 binds directly to downstream regulatory element (DRE) sites in gene promoters to repress transcription of targets including prodynorphin, GFAP, A20/TNFAIP3, and immunoglobulin-related genes, while Ca²⁺ binding releases it from DNA and promotes its association with Kv4 α-subunits (Kv4.2/Kv4.3), facilitating subunit tetramerization in the ER, surface trafficking, and generation of A-type potassium currents that regulate neuronal excitability and pacemaker frequency (PMID:11162748, PMID:15485870, PMID:11598014, PMID:16123112). KChIP3 additionally interacts with TRPV1 via its N-terminal residues 31–50 to reduce channel surface expression and Ca²⁺ influx, and Kcnip3 knockout in rats enhances inflammatory pain sensitivity and anxiety-like behavior, establishing KChIP3 as an endogenous restraint on nociception and negative affect (PMID:29335353, PMID:30740043). Through Ca²⁺-dependent interaction with hexokinase I, KChIP3 displaces hexokinase from mitochondria and promotes caspase-dependent apoptosis, and its transcriptional repression of A20 positions it as a pro-fibrotic factor whose loss protects against systemic sclerosis-like fibrosis (PMID:23524266, PMID:36289219).

Mechanistic history

Synthesis pass · year-by-year structured walk · 14 steps
  1. 2001 High

    Identifying KChIP3/DREAM as a Ca²⁺-regulated transcriptional repressor that binds DRE sites in the Ca²⁺-free state established the founding mechanism — a calcium-sensing switch controlling gene expression.

    Evidence In vitro DNA-binding assays, Ca²⁺-dependent transcriptional repression assays, EF-hand mutant analysis

    PMID:11162748

    Open questions at the time
    • Full repertoire of DRE-containing target genes unknown
    • Structural basis of Ca²⁺-induced conformational change releasing DNA not yet resolved at atomic level
    • Mechanism of nuclear import/export regulation unclear
  2. 2001 High

    Demonstrating that KChIP3 associates with Kv4.3 in dopaminergic neurons and that its mRNA level linearly determines A-type current density and pacemaker frequency established KChIP3 as a direct tuner of neuronal excitability, distinct from its transcriptional role.

    Evidence Single-cell RT-PCR quantification combined with patch-clamp electrophysiology in substantia nigra dopaminergic neurons

    PMID:11598014

    Open questions at the time
    • Whether KChIP3 splice variants differ in channel-modulatory capacity not resolved
    • Mechanism by which KChIP3 promotes channel trafficking versus gating modulation not separated
  3. 2001 Medium

    Showing that KChIP3 overexpression induces caspase-dependent apoptosis and that its knockdown attenuates Fas- and Ca²⁺-stress-induced cell death revealed a third functional axis — pro-apoptotic signaling.

    Evidence Antisense knockdown, overexpression, caspase activity assays, DNA laddering in cultured cells

    PMID:11259376

    Open questions at the time
    • Direct apoptotic effector mechanism (mitochondrial versus death-receptor pathway contribution) not dissected
    • Physiological relevance of KChIP3-mediated apoptosis in vivo not confirmed at this stage
  4. 2004 High

    Discovering that KChIP3 promotes Kv4.2 tetramerization within the ER — rescuing assembly-deficient Zn²⁺-site mutants — separated its role as a chaperone for subunit assembly from a general trafficking factor.

    Evidence Mutagenesis of Kv4.2 Zn²⁺-coordination residues, co-expression with KChIP3, electrophysiology and surface expression assays

    PMID:15485870

    Open questions at the time
    • Stoichiometry of KChIP3 in the assembled tetramer not definitively resolved
    • Whether KChIP3 dissociates after ER exit or remains in the surface complex unclear
  5. 2005 High

    Demonstrating that KChIP3, DPP10, and Kv4.2 form a ternary complex with distinct ISA-like kinetics showed that native A-type channels are multisubunit assemblies requiring both auxiliary proteins for physiological gating.

    Evidence Co-immunoprecipitation from rat brain and heterologous expression electrophysiology in oocytes and CHO cells

    PMID:16123112

    Open questions at the time
    • Cryo-EM or crystal structure of the full ternary complex not available
    • Whether DPP10 and KChIP3 compete for overlapping binding sites on Kv4.2 not addressed
  6. 2009 Medium

    KChIP3 knockout mice showing enhanced contextual fear memory — with a shift from membrane-associated (Kv4-bound) to nuclear KChIP3 after fear conditioning — revealed that activity-dependent redistribution between channel modulation and transcriptional repression is a physiological regulatory mechanism during memory consolidation.

    Evidence KChIP3 knockout mouse fear conditioning, subcellular fractionation, co-immunoprecipitation, RT-PCR for prodynorphin

    PMID:19223600

    Open questions at the time
    • Signaling pathway controlling KChIP3 membrane-to-nucleus translocation not identified
    • Contribution of prodynorphin repression versus Kv4 modulation to the memory phenotype not separated
  7. 2009 Medium

    Identifying DREAM/KChIP3 as a direct interactor of the TSH receptor that enhances Gαs coupling, with transgenic overexpression causing thyroid enlargement mirrored in human goiters, extended KChIP3 function to GPCR signaling outside the nervous system.

    Evidence Protein-protein interaction assay, transgenic mouse model, immunohistochemistry on human multinodular goiter tissue

    PMID:19299442

    Open questions at the time
    • Binding interface between DREAM and TSHR not mapped
    • Whether KChIP3 modulates other GPCRs not tested
  8. 2009 Medium

    Placing KChIP3 on the GFAP promoter DRE as a mediator of PACAP–cAMP–Ca²⁺-driven astrogliogenesis, with KO mice showing altered neuron/astrocyte ratios, established a developmental role for DREAM-mediated transcriptional control.

    Evidence Reporter assays, DREAM knockout mice developmental phenotype, pharmacological cAMP/Ca²⁺ manipulation

    PMID:19238593

    Open questions at the time
    • Whether DREAM represses or activates GFAP depending on Ca²⁺ state not fully dissected
    • Cell-type-specific conditional KO not performed
  9. 2010 High

    Showing that KChIP2, KChIP3, and KChIP4 are interdependently required for cortical IA currents, with compensatory upregulation upon single-gene loss, revealed functional redundancy among KChIP family members in vivo.

    Evidence Co-immunoprecipitation from mouse cortex, KChIP2/3 knockout and RNAi triple-knockdown electrophysiology

    PMID:20943905

    Open questions at the time
    • Unique versus redundant contributions of each KChIP isoform to channel kinetics not fully separated
    • Triple KO animal not generated
  10. 2013 Medium

    Mapping the Ca²⁺-dependent interaction of KChIP3 with hexokinase I and showing that KChIP3 displaces hexokinase from mitochondria to promote caspase-3 activation provided a molecular mechanism for the previously observed pro-apoptotic activity.

    Evidence Co-immunoprecipitation, deletion/Ca²⁺-binding mutants, caspase-3 activity assays, mitochondrial fractionation

    PMID:23524266

    Open questions at the time
    • In vivo validation of hexokinase displacement during physiological apoptosis not performed
    • Whether other KChIP family members share this pro-apoptotic mechanism unknown
  11. 2014 High

    Biophysical characterization of KChIP3–Kv4.3 binding as Ca²⁺-dependent and identification of Tyr-174/Phe-218 as critical residues for drug-enhanced interaction provided the first quantitative binding model for the KChIP3–Kv4 interface.

    Evidence Fluorescence spectroscopy, isothermal calorimetry, docking simulations, site-directed mutagenesis

    PMID:25228688

    Open questions at the time
    • High-resolution co-crystal structure of KChIP3–Kv4.3 complex not obtained
    • Role of these residues in native neuronal channels not tested
  12. 2018 High

    Demonstrating that the KChIP3 N-terminal fragment (aa 31–50) binds TRPV1 intracellular domains to reduce channel surface expression and that a cell-permeable version of this peptide alleviates inflammatory hyperalgesia in vivo established KChIP3 as an endogenous negative regulator of pain-channel trafficking.

    Evidence Co-immunoprecipitation, peptide competition, Ca²⁺ imaging, Kcnip3 KO rat model, CFA inflammatory pain model with TAT-peptide rescue

    PMID:29335353

    Open questions at the time
    • Structural basis of the 31–50 peptide–TRPV1 interaction not resolved
    • Whether KChIP3 regulates TRPV1 gating in addition to trafficking not determined
  13. 2019 Medium

    Global Kcnip3 KO rats exhibiting enhanced pain sensitivity, anxiety, and depression alongside cortical transcriptomic changes in dopaminergic genes confirmed KChIP3 as a broad restraint on nociception and negative affect, acting through transcriptional regulation.

    Evidence Kcnip3 KO rats, nociceptive/affective behavioral assays, RNA-Seq and qPCR

    PMID:30740043

    Open questions at the time
    • Relative contribution of Kv4 channel modulation versus transcriptional repression to the pain/affect phenotype not dissected
    • Circuit-level mechanisms linking cortical transcriptomic changes to behavior not identified
  14. 2022 High

    Establishing that DREAM transcriptionally represses A20/TNFAIP3 and that DREAM-null mice are protected from fibrosis while A20-deficient fibroblasts develop SSc-like fibrosis defined a DREAM→A20 axis controlling extracellular matrix homeostasis, extending KChIP3 function to fibrotic disease.

    Evidence DREAM KO and fibroblast-specific A20 KO mice, ChIP, gene expression, TGF-β stimulation, bleomycin fibrosis model, human SSc tissue

    PMID:36289219

    Open questions at the time
    • Whether the DREAM–A20 axis operates in other fibrotic organs beyond skin not tested
    • Direct DRE site in the A20 promoter not mapped at nucleotide resolution

Open questions

Synthesis pass · forward-looking unresolved questions
  • A unified structural and signaling model explaining how Ca²⁺ binding simultaneously controls KChIP3's partitioning among DNA, Kv4 channels, TRPV1, hexokinase I, and TSHR — and whether these interactions are mutually exclusive or context-dependent — remains unresolved.
  • No high-resolution structure of full-length KChIP3 in complex with any partner
  • Quantitative model of Ca²⁺-dependent partitioning among multiple interaction partners absent
  • Cell-type-specific conditional knockouts needed to separate transcriptional from channel-modulatory functions in vivo

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 6 GO:0140110 transcription regulator activity 6 GO:0003677 DNA binding 5 GO:0140299 molecular sensor activity 2
Localization
GO:0005634 nucleus 5 GO:0005886 plasma membrane 5 GO:0005783 endoplasmic reticulum 1
Pathway
R-HSA-112316 Neuronal System 7 R-HSA-74160 Gene expression (Transcription) 4 R-HSA-162582 Signal Transduction 2 R-HSA-168256 Immune System 2 R-HSA-5357801 Programmed Cell Death 2
Partners
DPP10HK1KCND2KCND3KCNIP2KCNIP4TRPV1TSHR
Complex memberships
Kv4.2/KChIP3 channel complexKv4.2/KChIP3/DPP10 ternary complexKv4.3/KChIP3 channel complex

Evidence

Reading pass · 17 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 KChIP3/DREAM is a Ca2+-regulated transcriptional repressor that binds directly to downstream regulatory element (DRE) sites in DNA promoters; binding and repression occur in the Ca2+-free state, and elevated Ca2+ causes derepression by releasing DREAM from DNA. In vitro DNA-binding assays, Ca2+-dependent transcriptional repression assays, EF-hand mutant analysis Seminars in cell & developmental biology High 11162748
2001 KChIP3 (KChip3.1 long splice variant) associates with Kv4.3 α-subunits to form A-type potassium channels in dopaminergic substantia nigra neurons; the number of KChIP3 mRNA molecules in individual neurons correlates linearly with A-type channel density and pacemaker frequency, establishing transcriptional control of KChIP3 as a mechanism for tuning firing rate. Single-cell RT-PCR quantification combined with patch-clamp electrophysiology in identified dopaminergic neurons The EMBO journal High 11598014
2001 Calsenilin/DREAM/KChIP3 has a pro-apoptotic function: its overexpression induces apoptosis (cell shrinkage, DNA laddering, caspase activation), and antisense-mediated knockdown attenuates apoptosis induced by Fas, Ca2+-ionophore, or thapsigargin. Apoptosis was suppressed by caspase inhibitor Z-VAD and Bcl-XL. KChIP3 expression also increased Aβ42 production in cells co-expressing APPsw and presenilin 2. Antisense oligonucleotide knockdown, overexpression, caspase activity assays, DNA laddering, morphological analysis FASEB journal Medium 11259376
2004 Induction of calsenilin/DREAM/KChIP3 is observed in Alzheimer's disease cortex and in APP transgenic mouse brain; exposure of cultured neurons to Aβ42 induces KChIP3 protein and mRNA, and blocking KChIP3 expression protects neurons from Aβ toxicity, establishing a role for KChIP3 in Aβ-driven neurodegeneration. Immunohistochemistry on AD brain tissue, cortical neuron culture with Aβ42 treatment, antisense knockdown, cell viability assays Journal of neurochemistry Medium 14720210
2005 KChIP3 and DPP10 associate simultaneously with Kv4.2 to form a ternary Kv4.2/KChIP3/DPP10 macromolecular complex; this ternary complex produces ISA-like currents with very rapid recovery from inactivation (~18–26 ms) distinct from binary Kv4.2+KChIP3 or Kv4.2+DPP10 channels. Co-immunoprecipitation from rat brain and Xenopus oocytes, heterologous co-expression electrophysiology in oocytes and CHO cells The Journal of physiology High 16123112
2004 KChIP3 promotes Kv4.2 subunit assembly into tetramers by binding monomeric subunits within the endoplasmic reticulum; KChIP3 rescues functional channel expression of Kv4.2 tetramerization-deficient mutants (Zn2+ site mutants) that are otherwise trapped in the ER, demonstrating a direct role in subunit assembly beyond trafficking. Mutagenesis of Zn2+-coordination residues in Kv4.2, co-expression with KChIP3, electrophysiology, surface expression assays The Journal of biological chemistry High 15485870
2009 KChIP3/DREAM regulates contextual fear memory consolidation: KChIP3 knockout mice show enhanced contextual fear memory. After fear conditioning, membrane association of KChIP3 and its interaction with Kv4.2 decrease, nuclear KChIP3 increases, and prodynorphin mRNA expression decreases in WT but not KO animals, indicating a shift from channel modulation to transcriptional repression. KChIP3 knockout mouse behavior (contextual fear conditioning), subcellular fractionation, co-immunoprecipitation, RT-PCR Learning & memory Medium 19223600
2009 DREAM/KChIP3 modulates TSH receptor (TSHR) activity through direct protein-protein interaction that promotes coupling between TSHR and Gαs; DREAM overexpression in transgenic mice causes marked thyroid enlargement, and elevated DREAM protein is found in human multinodular goiters. Protein-protein interaction assay, transgenic mouse model with thyroid phenotype, immunohistochemistry on human goiter tissue Molecular endocrinology Medium 19299442
2009 PACAP-cAMP signaling promotes astrogliogenesis via a pathway in which cAMP-dependent extracellular Ca2+ entry activates DREAM/KChIP3, which is bound to DRE sites in the GFAP gene promoter, stimulating GFAP expression during astrocyte differentiation. DREAM knockout in vivo alters the numbers of neurons and astrocytes generated during development. Reporter assays, DREAM knockout mice with developmental phenotype, pharmacological manipulations of cAMP and Ca2+ Molecular neurobiology Medium 19238593
2010 KChIP3 coimmunoprecipitates with Kv4.2 in adult mouse cortex; KChIP2, KChIP3, and KChIP4 together are required interdependently for generation of IA currents in cortical pyramidal neurons — loss of KChIP3 alone reduces IA density modestly but triggers compensatory upregulation of KChIP2 and KChIP4. Co-immunoprecipitation, KChIP2/3 knockout mice electrophysiology, RNAi triple knockdown of KChIP2/3/4 The Journal of neuroscience High 20943905
2010 DREAM/KChIP3 acts as a transcriptional repressor controlling B cell proliferation and Ig synthesis; transgenic mice expressing dominant-active DREAM show reduced serum Ig levels due to reduced mRNA translation (linked to decreased Eif4g3) and accelerated B cell division correlated with reduced Klf9 expression. Transgenic dominant-active DREAM mice, in vitro B cell assays, transcriptomics, pulse-chase protein synthesis experiments Journal of immunology Medium 21059893
2013 In the presence of Ca2+, DREAM/KChIP3 interacts directly with hexokinase I; this interaction requires the N-terminal 94 amino acids of DREAM and Ca2+ binding. DREAM overexpression reduces hexokinase I localization on mitochondria and enhances cisplatin-mediated caspase-3 activity; co-expression of hexokinase I reduces DREAM-induced apoptosis. Co-immunoprecipitation, deletion mutants, Ca2+-binding mutants, caspase-3 activity assays, mitochondrial fractionation Biochemical and biophysical research communications Medium 23524266
2014 NS5806, a Kv4 current activator, binds at a hydrophobic site on the C-terminus of KChIP3 in a calcium-dependent manner (Kd ~2–5 μM calcium-bound form). KChIP3 association with the Kv4.3 N-terminus is itself calcium-dependent. NS5806 increases KChIP3–Kv4.3 affinity and decreases their dissociation rate; Tyr-174 and Phe-218 on KChIP3 are required for drug-enhanced Kv4.3 binding. Fluorescence spectroscopy, isothermal calorimetry, docking simulations, site-directed mutagenesis The Journal of biological chemistry High 25228688
2018 The N-terminal 31–50 fragment of KChIP3 binds directly to both intracellular N- and C-termini of TRPV1, reducing TRPV1 surface localization and Ca2+ influx through the channel. Intrathecal or intraplantar delivery of a TAT-31–50 transmembrane peptide reduces TRPV1-mediated Ca2+ influx in DRG neurons and alleviates inflammatory thermal hyperalgesia in rats. Co-immunoprecipitation, co-localization, peptide competition, Ca2+ imaging, kcnip3-/- rat model (KO validation), complete Freund's adjuvant inflammatory pain model The Journal of neuroscience High 29335353
2016 Chronic ethanol exposure reduces hippocampal Kv4.2 and KChIP3 protein levels while increasing FMRP phosphorylation at S499 (via S6K1), which increases FMRP translational suppression of Kv4.2 and KChIP3 mRNAs; S6K1 inhibition prevents both the increase in phospho-FMRP and the reduction of Kv4.2/KChIP3 protein. Western blotting, FMRP-mRNA pulldown from hippocampal slice cultures, pharmacological S6K1 inhibition Alcoholism, clinical and experimental research Medium 27147118
2019 Global Kcnip3 knockout in rats enhances acute and chronic inflammatory pain sensitivity, increases anxiety and depression-like behavior, and causes transcriptional changes in forebrain cortex including upregulation of dopaminergic transmission genes (Nr4a2, Ret, Cplx3, Rgs9) and downregulation of others (Col3a1, Ddc), indicating KChIP3 normally restrains pain and negative emotional processing through transcriptional control. Kcnip3 knockout rats, nociceptive behavioral assays, RNA-Seq transcriptomics, qPCR validation Frontiers in molecular neuroscience Medium 30740043
2022 DREAM/KChIP3 transcriptionally represses A20 (TNFAIP3) in fibroblasts; DREAM-null mice have elevated A20 expression and are protected from fibrosis, while A20-haploinsufficient or fibroblast-specific A20-deleted mice develop SSc-like fibrosis. TGF-β induces A20 in DREAM-null but not wild-type fibroblasts, placing DREAM upstream of A20 in a fibrosis regulatory axis. DREAM knockout mice, fibroblast-specific A20 knockout mice, ChIP/gene expression assays, TGF-β stimulation, bleomycin fibrosis model Nature communications High 36289219

Source papers

Stage 0 corpus · 100 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2017 Cell cycle arrest through indirect transcriptional repression by p53: I have a DREAM. Cell death and differentiation 520 29125603
2015 The p53-p21-DREAM-CDE/CHR pathway regulates G2/M cell cycle genes. Nucleic acids research 278 26384566
2001 Tuning pacemaker frequency of individual dopaminergic neurons by Kv4.3L and KChip3.1 transcription. The EMBO journal 245 11598014
2007 Dialogue on reverse-engineering assessment and methods: the DREAM of high-throughput pathway inference. Annals of the New York Academy of Sciences 223 17925349
2021 Dream: powerful differential expression analysis for repeated measures designs. Bioinformatics (Oxford, England) 219 32730587
2017 Cell cycle transcription control: DREAM/MuvB and RB-E2F complexes. Critical reviews in biochemistry and molecular biology 197 28799433
2017 Angiogenic gene therapy in cardiovascular diseases: dream or vision? European heart journal 141 28073865
2016 Peroxygenases en route to becoming dream catalysts. What are the opportunities and challenges? Current opinion in chemical biology 132 27992798
2014 Circulating tumor cells and circulating tumor DNA for precision medicine: dream or reality? Annals of oncology : official journal of the European Society for Medical Oncology 120 25336116
2005 Multiprotein assembly of Kv4.2, KChIP3 and DPP10 produces ternary channel complexes with ISA-like properties. The Journal of physiology 115 16123112
2021 Allogeneic CAR Cell Therapy-More Than a Pipe Dream. Frontiers in immunology 104 33488631
2015 Structural mechanisms of DREAM complex assembly and regulation. Genes & development 96 25917549
2016 Targeting the PD-1/PD-L1 axis in multiple myeloma: a dream or a reality? Blood 90 27919908
2024 Molecular glues for protein-protein interactions: Progressing toward a new dream. Cell chemical biology 87 38701786
2017 Human papilloma virus E7 oncoprotein abrogates the p53-p21-DREAM pathway. Scientific reports 86 28572607
2013 Tolerogenic dendritic cell vaccines to treat autoimmune diseases: can the unattainable dream turn into reality? Autoimmunity reviews 77 24120737
2011 Crowdsourcing network inference: the DREAM predictive signaling network challenge. Science signaling 76 21900204
2002 L-arginine in cardiovascular disease: dream or reality? Vascular medicine (London, England) 69 12553744
2001 Pro-apoptotic function of calsenilin/DREAM/KChIP3. FASEB journal : official publication of the Federation of American Societies for Experimental Biology 65 11259376
2022 Association Between Depression and Severity of Dry Eye Symptoms, Signs, and Inflammatory Markers in the DREAM Study. JAMA ophthalmology 61 35266971
2008 Vaccine adjuvants: the dream becomes real. Human vaccines 55 18682690
2018 The cell cycle regulatory DREAM complex is disrupted by high expression of oncogenic B-Myb. Oncogene 54 30206359
2004 Induction of pro-apoptotic calsenilin/DREAM/KChIP3 in Alzheimer's disease and cultured neurons after amyloid-beta exposure. Journal of neurochemistry 54 14720210
2015 Reconstructing each cell's genome within complex microbial communities-dream or reality? Frontiers in microbiology 53 25620966
2015 The DREAM complex promotes gene body H2A.Z for target repression. Genes & development 52 25737279
2000 Drugs selective for nicotinic receptor subtypes: a real possibility or a dream? Behavioural brain research 52 10942044
2021 PAF remodels the DREAM complex to bypass cell quiescence and promote lung tumorigenesis. Molecular cell 50 33626321
2014 Universal influenza vaccines, a dream to be realized soon. Viruses 49 24784572
2009 The role of calsenilin/DREAM/KChIP3 in contextual fear conditioning. Learning & memory (Cold Spring Harbor, N.Y.) 49 19223600
2020 Antibodies for the Treatment of Brain Metastases, a Dream or a Reality? Pharmaceutics 48 31940974
2010 Interdependent roles for accessory KChIP2, KChIP3, and KChIP4 subunits in the generation of Kv4-encoded IA channels in cortical pyramidal neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience 48 20943905
2016 The cave microbiome as a source for drug discovery: Reality or pipe dream? Biochemical pharmacology 47 27867014
2023 The DREAM complex functions as conserved master regulator of somatic DNA-repair capacities. Nature structural & molecular biology 46 36959262
2021 TMB in NSCLC: A Broken Dream? International journal of molecular sciences 46 34207126
2001 Ca2+-dependent transcriptional repression and derepression: DREAM, a direct effector. Seminars in cell & developmental biology 44 11162748
2021 Cancer Vaccines: Promising Therapeutics or an Unattainable Dream. Vaccines 42 34207062
2007 Breast cancer vaccines: promise for the future or pipe dream? Cancer 42 17763371
2021 Advances in systems biology modeling: 10 years of crowdsourcing DREAM challenges. Cell systems 41 34139170
2020 Multiple Myeloma DREAM Challenge reveals epigenetic regulator PHF19 as marker of aggressive disease. Leukemia 41 32060406
2021 The DREAM complex represses growth in response to DNA damage in Arabidopsis. Life science alliance 38 34583930
2008 Immunohistochemical assessment of protein phosphorylation state: the dream and the reality. Histochemistry and cell biology 37 18648845
2004 KChIP3 rescues the functional expression of Shal channel tetramerization mutants. The Journal of biological chemistry 37 15485870
2023 Key elements of cellular senescence involve transcriptional repression of mitotic and DNA repair genes through the p53-p16/RB-E2F-DREAM complex. Aging 35 37219418
2017 Invention and Early History of Morpholinos: From Pipe Dream to Practical Products. Methods in molecular biology (Clifton, N.J.) 35 28364229
2021 DREAM On: Cell Cycle Control in Development and Disease. Annual review of genetics 34 34496610
2014 DNA testing for malignant hyperthermia: the reality and the dream. Anesthesia and analgesia 34 24445638
2006 Adult stem cell therapy: dream or reality? Transplant immunology 34 17157222
2024 The concept of gene therapy for glaucoma: the dream that has not come true yet. Neural regeneration research 33 37488850
2018 Checkpoint-inhibition in ovarian cancer: rising star or just a dream? Journal of gynecologic oncology 31 30207101
2012 Pharma-metabolomics in neonatology: is it a dream or a fact? Current pharmaceutical design 31 22564294
2018 The HDAC-Associated Sin3B Protein Represses DREAM Complex Targets and Cooperates with APC/C to Promote Quiescence. Cell reports 30 30517867
2020 Cardiac Regeneration: New Hope for an Old Dream. Annual review of physiology 29 33064963
2019 The Dream of a Mycobacterium. Microbiology spectrum 29 31025625
2017 Using binding competitors of albumin to promote the removal of protein-bound uremic toxins in hemodialysis: Hope or pipe dream? Biochimie 29 28987629
2022 Disrupting the DREAM complex enables proliferation of adult human pancreatic β cells. The Journal of clinical investigation 28 35700053
2020 Oxidative Stress and Inflammation as Predictors of Mortality and Cardiovascular Events in Hemodialysis Patients: The DREAM Cohort. Journal of atherosclerosis and thrombosis 28 32741893
2020 Measuring Extracellular Vesicles by Conventional Flow Cytometry: Dream or Reality? International journal of molecular sciences 28 32872424
2014 Modulation of the voltage-gated potassium channel (Kv4.3) and the auxiliary protein (KChIP3) interactions by the current activator NS5806. The Journal of biological chemistry 28 25228688
2014 Loss of the mammalian DREAM complex deregulates chondrocyte proliferation. Molecular and cellular biology 27 24710275
2022 Shorter antibiotic courses in the immunocompromised: the impossible dream? Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases 26 35988852
2021 PD-L1 expression as a predictive biomarker for immune checkpoint inhibitors: between a dream and a nightmare. Immunotherapy 24 34190579
2014 Do endothelial cells dream of eclectic shape? Developmental cell 24 24780735
2017 The Endocannabinoid System Modulating Levels of Consciousness, Emotions and Likely Dream Contents. CNS & neurological disorders drug targets 23 28240187
2014 dREAM co-operates with insulator-binding proteins and regulates expression at divergently paired genes. Nucleic acids research 23 25053843
2009 PACAP signaling to DREAM: a cAMP-dependent pathway that regulates cortical astrogliogenesis. Molecular neurobiology 22 19238593
2009 The DREAM protein is associated with thyroid enlargement and nodular development. Molecular endocrinology (Baltimore, Md.) 22 19299442
2012 The role of microvesicles derived from mesenchymal stem cells in tissue regeneration; a dream for tendon repair? Muscles, ligaments and tendons journal 21 23738299
2010 Increased B cell proliferation and reduced Ig production in DREAM transgenic mice. Journal of immunology (Baltimore, Md. : 1950) 20 21059893
2023 Daring to dream: Targeting lipoprotein(a) as a causal and risk-enhancing factor. Pharmacological research 19 37406784
2021 Progesterone Receptors Promote Quiescence and Ovarian Cancer Cell Phenotypes via DREAM in p53-Mutant Fallopian Tube Models. The Journal of clinical endocrinology and metabolism 19 33755733
2018 KChIP3 N-Terminal 31-50 Fragment Mediates Its Association with TRPV1 and Alleviates Inflammatory Hyperalgesia in Rats. The Journal of neuroscience : the official journal of the Society for Neuroscience 19 29335353
2018 Review: Limb regeneration in humans: Dream or reality? Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 19 29408367
2024 The p21CIP1-CDK4-DREAM axis is a master regulator of genotoxic stress-induced cellular senescence. Nucleic acids research 18 38783095
2023 Novel technologies are turning a dream into reality: conditionally replicating viruses as vaccines. Trends in microbiology 18 37798168
2022 Fibroblast A20 governs fibrosis susceptibility and its repression by DREAM promotes fibrosis in multiple organs. Nature communications 18 36289219
2017 Organ reconstruction: Dream or reality for the future. Bio-medical materials and engineering 18 28372287
2016 Capturing Darwin's dream. Molecular ecology resources 18 27454358
2020 Monoclonal Antibodies to Treat Multiple Myeloma: A Dream Come True. International journal of molecular sciences 17 33139668
2019 Electroacupuncture regulates the DREAM/NF-κB signalling pathway and ameliorates cyclophosphamide-induced immunosuppression in mice. Acupuncture in medicine : journal of the British Medical Acupuncture Society 17 31192694
2016 FMRP Mediates Chronic Ethanol-Induced Changes in NMDA, Kv4.2, and KChIP3 Expression in the Hippocampus. Alcoholism, clinical and experimental research 17 27147118
2022 Plasmodium vivax Duffy Binding Protein-Based Vaccine: a Distant Dream. Frontiers in cellular and infection microbiology 16 35909975
2022 Therapeutic Potential of VEGF-B in Coronary Heart Disease and Heart Failure: Dream or Vision? Cells 16 36552897
2021 p53-Dependent Repression: DREAM or Reality? Cancers 16 34638334
2021 DREAM: a database of experimentally supported protein-coding RNAs and drug associations in human cancer. Molecular cancer 16 34774049
2015 The association of mammalian DREAM complex and HPV16 E7 proteins. American journal of cancer research 16 26885443
2011 Building the DREAM interactome. Science China. Life sciences 16 21786202
2008 Cholesteryl ester transfer protein inhibition and HDL increase: has the dream ended? Expert opinion on investigational drugs 16 18363511
2023 Vaccinal effect of HIV-1 antibody therapy: dream or reality? Current opinion in HIV and AIDS 15 37144564
2023 Would doctors dream of electric blood bankers? Large language model-based artificial intelligence performs well in many aspects of transfusion medicine. Transfusion 15 37644845
2019 Global Gene Knockout of Kcnip3 Enhances Pain Sensitivity and Exacerbates Negative Emotions in Rats. Frontiers in molecular neuroscience 15 30740043
2018 Molecular characterization and biomarker identification in colorectal cancer: Toward realization of the precision medicine dream. Cancer management and research 15 30510457
2009 Universal species concept: pipe dream or a step toward unifying biology? Journal of industrial microbiology & biotechnology 15 19779746
2024 PCLAF-DREAM drives alveolar cell plasticity for lung regeneration. Nature communications 14 39448571
2021 Untapped endocannabinoid pharmacological targets: Pipe dream or pipeline? Pharmacology, biochemistry, and behavior 14 33932409
2015 Does Arabidopsis thaliana DREAM of cell cycle control? The EMBO journal 14 26089020
2013 Chemoprevention of gastrointestinal cancer: the reality and the dream. Gut and liver 14 23560148
2011 Development of neuromodulation treatments in a large animal model--do neurosurgeons dream of electric pigs? Progress in brain research 14 21867797
2007 Lipid rafts: dream or reality for cholesterol transporters? European biophysics journal : EBJ 14 17576551
2014 Lab generated retina: realizing the dream. Visual neuroscience 13 24847731
2013 The regulation of apoptosis by the downstream regulatory element antagonist modulator/potassium channel interacting protein 3 (DREAM/KChIP3) through interactions with hexokinase I. Biochemical and biophysical research communications 13 23524266