Affinage

IFI27

Interferon alpha-inducible protein 27, mitochondrial · UniProt P40305

Length
122 aa
Mass
11.5 kDa
Annotated
2026-06-10
56 papers in source corpus 19 papers cited in narrative 19 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

IFI27 (ISG12) is a small interferon-stimulated hydrophobic protein that integrates mitochondrial metabolism, apoptosis, and antiviral innate immunity, acting as a context-dependent regulator of cell fate and host defense (PMID:27673746, PMID:37544897, PMID:37187533). At mitochondria, full-length ISG12A sensitizes cells to apoptotic stimuli and directly induces apoptosis through two BH3-like motifs, co-immunoprecipitating with Bcl-2 and forming homodimers consistent with membrane pore formation (PMID:27673746). In a non-apoptotic role at the same organelle, IFI27 sustains mitochondrial function: it physically binds SDHB and links it to the chaperone TRAP1 to protect succinate dehydrogenase from degradation, and binds and activates HADHA to drive fatty acid β-oxidation, supporting cristae morphogenesis and adaptive thermogenesis in brown adipocytes (PMID:37544897, PMID:29730295). In innate immunity IFI27 acts as a negative regulator of cytoplasmic RNA sensing, binding nucleic acids and interacting with RIG-I and MDA5 to impair their activation—competing with MDA5 for poly(I:C) and blocking its oligomerization—while conversely potentiating PKR through a PACT-dependent interaction to increase eIF2α phosphorylation and stress granule formation (PMID:37187533, PMID:39431052, PMID:40522981). IFI27 also exerts direct antiviral activity by routing viral and host proteins to the proteasome: it promotes SKP2-dependent ubiquitination and degradation of HIV-1 Gag (p24/p55) and of the HBV-supporting transcription factor C/EBPα (PMID:39475279, PMID:41081508). As an interferon target, IFI27 expression is transcriptionally repressed by ATF3 binding its promoter and its protein is stabilized by USP18-mediated deubiquitination, coupling it to interferon and tumor-associated signaling (PMID:33539340, PMID:39993992). Reported localization spans the nuclear envelope, mitochondria, and cytoplasm across cell contexts (PMID:11722583, PMID:27673746, PMID:41081508).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 2001 Medium

    Established the first subcellular address for the interferon-induced ISG12/IFI27 protein, anchoring it to the nuclear envelope.

    Evidence Subcellular fractionation of recombinant tagged protein and immunocytochemistry in IFN-alpha-treated HeLa cells

    PMID:11722583

    Open questions at the time
    • Does not assign molecular function
    • Later work places IFI27 at mitochondria and cytoplasm, leaving localization context-dependent and unresolved
  2. 2013 Medium

    Linked IFI27 to innate immune regulation by showing it drives nuclear export of nuclear receptors to limit their anti-inflammatory activity, with genetic epistasis placing it upstream of NR4A1.

    Evidence ISG12-knockout mice in sepsis/endotoxemia models, macrophage assays, and NR4A1 double-knockout rescue

    PMID:23747037

    Open questions at the time
    • Direct interaction interface with nuclear receptors not mapped
    • Mechanism connecting NR export to IL-6 not detailed
  3. 2015 Medium

    Defined a cell-cycle/proliferation role, showing IFI27 loss imposes S-phase arrest via CDK1 inhibition and p53/p21 activation, and that EGF stabilizes IFI27 protein.

    Evidence siRNA/overexpression in keratinocytes with flow cytometry, cyclin A/CDK1 Co-IP, and an imiquimod psoriasis mouse model

    PMID:25664647

    Open questions at the time
    • Direct molecular target of IFI27 in the cell cycle not identified
    • Mechanism of EGF-mediated stabilization unknown
  4. 2016 High

    Provided the strongest mechanistic case for a pro-apoptotic mitochondrial function, identifying BH3-like motifs and Bcl-2 binding.

    Evidence Overexpression in HEK293, BH3 mutagenesis, Bcl-2 Co-IP, and mitochondrial fractionation/imaging

    PMID:27673746

    Open questions at the time
    • Homodimer pore-formation model not structurally confirmed
    • How apoptotic and metabolic mitochondrial roles are partitioned is unclear
  5. 2018 Medium

    Revealed a metabolic mitochondrial function, showing IFI27 supports TCA/ETC gene expression and is required for adrenergic browning of white fat.

    Evidence Mitochondrial imaging/fractionation, siRNA/overexpression in brown adipocytes, and in vivo beta3-agonist treatment

    PMID:29730295

    Open questions at the time
    • Molecular mechanism linking IFI27 to mitochondrial gene programs not yet defined here
    • Direct partners not identified at this stage
  6. 2020 Medium

    Generalized the nuclear-export function to estrogen receptor alpha, showing IFI27 bridges ERalpha to CRM1/XPO1 to attenuate ERalpha transactivation.

    Evidence Reciprocal Co-IP of IFI27 with ERalpha and CRM1/XPO1, reporter assays, and proliferation/migration assays in MCF-7 cells

    PMID:33117284

    Open questions at the time
    • Direct binding interface not mapped
    • Whether export is direct cargo adaptation or indirect is unresolved
  7. 2021 High

    Identified direct transcriptional control of IFI27, with ATF3 binding its promoter to repress expression and restrain tumor cell growth.

    Evidence ChIP, RNA-seq, ATF3 gain/loss in TSCC lines, IFI27 knockdown, and in vivo rescue

    PMID:33539340

    Open questions at the time
    • Upstream signals controlling ATF3 occupancy not defined
    • Downstream IFI27 effectors in TSCC not mapped
  8. 2022 Medium

    Established post-transcriptional and post-translational stabilization layers controlling IFI27 abundance via mRNA stability, m6A, miRNA, and deubiquitination.

    Evidence PABPC1/eIF4G Co-IP and mRNA stability assays (ESCC); USP18 Co-IP/deubiquitination with HOXA5 ChIP; miR-942-5p 3'-UTR reporter assays

    PMID:35346324 PMID:35974384 PMID:39993992

    Open questions at the time
    • Relative contribution of each regulatory layer in vivo unknown
    • How these inputs are integrated under interferon signaling not resolved
  9. 2023 High

    Defined the dual mitochondrial-metabolic and antiviral-sensing functions: IFI27 protects SDHB via TRAP1 and activates HADHA, while binding RIG-I to suppress cytoplasmic RNA sensing.

    Evidence Interaction mapping and Co-IP with SDHB/HADHA/TRAP1 plus Ifi27-knockout metabolic phenotyping; RIG-I Co-IP with KD/OE in IAV/SARS-CoV-2/SeV infection

    PMID:37187533 PMID:37544897

    Open questions at the time
    • Structural basis of SDHB-TRAP1 bridging not solved
    • Whether RIG-I suppression is purely RNA-mediated not fully resolved
  10. 2024 High

    Extended antiviral mechanisms: IFI27 blocks MDA5 oligomerization by competing for dsRNA and targets HIV-1 Gag for SKP2/K48-ubiquitin proteasomal degradation via mapped domains.

    Evidence MDA5 Co-IP and poly(I:C) competition assays; Gag (p24/p55) Co-IP, domain mapping, SKP2 identification, and ubiquitin-proteasome/HIV replication assays

    PMID:39431052 PMID:39475279

    Open questions at the time
    • Reconciling immune-suppressive (RIG-I/MDA5) and antiviral (Gag degradation) roles mechanistically
    • Selectivity of SKP2 recruitment by IFI27 unexplained
  11. 2025 High

    Completed the antiviral/immune picture with SKP2-dependent C/EBPalpha degradation restricting HBV and PACT-dependent PKR potentiation enhancing translational stress responses.

    Evidence HBV ubiquitination/SKP2-rescue assays in HepG2-NTCP and hepatocytes; PACT/PKR Co-IP with eIF2alpha phosphorylation and stress-granule readouts in SARS-CoV-2/VSV models

    PMID:40522981 PMID:41081508

    Open questions at the time
    • How IFI27 selects SKP2 substrates (Gag vs C/EBPalpha) is unknown
    • Net effect on interferon signaling of simultaneous PRR suppression and PKR activation unresolved

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unknown how a single small protein coordinates its opposing roles—pro-apoptotic versus metabolism-sustaining at mitochondria, and PRR-suppressive versus PKR-activating in immunity—and what structural features dictate which function dominates in a given cell type.
  • No structural model of IFI27 or its complexes
  • No unifying framework for context-dependent function selection
  • Localization determinants (nuclear envelope vs mitochondria vs cytoplasm) not defined

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0098772 molecular function regulator activity 4 GO:0003723 RNA binding 3 GO:0060090 molecular adaptor activity 2
Localization
GO:0005739 mitochondrion 3 GO:0005635 nuclear envelope 1 GO:0005829 cytosol 1
Pathway
R-HSA-168256 Immune System 3 R-HSA-1430728 Metabolism 2 R-HSA-392499 Metabolism of proteins 2 R-HSA-5357801 Programmed Cell Death 1
Partners
BCL-2HADHAMDA5PRKRARIG-ISDHBTRAP1XPO1

Evidence

Reading pass · 19 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2001 ISG12/IFI27 protein localizes to the nuclear envelope (nuclear membrane), as demonstrated by baculovirus-expressed and FLAG-tagged recombinant ISG12 sedimentation in nuclear envelope fractions and immunocytochemical detection in IFN-alpha-treated HeLa cells. Subcellular fractionation of baculovirus-expressed and FLAG-tagged recombinant protein in HEK293 cells; immunocytochemistry in HeLa cells treated with IFN-alpha European journal of biochemistry Medium 11722583
2013 ISG12 (IFI27) directly interacts with nuclear receptors (NRs) and acts as a co-factor stimulating nuclear export of NRs, thereby reducing the anti-inflammatory potential of NRs such as NR4A1. ISG12-deficient mice showed prolonged survival and reduced IL-6 levels in experimental sepsis, and this phenotype was restored in ISG12/NR4A1 double-deficient mice, placing ISG12 upstream of NR4A1 in innate immune regulation. ISG12-knockout mice in cecal ligation and puncture (CLP) and endotoxemia models; in vitro peritoneal macrophage experiments; genetic epistasis with NR4A1 double-knockout mice; direct interaction assay with NRs Immunobiology Medium 23747037
2015 IFI27 knockdown in keratinocytes reduces proliferation and causes S-phase arrest associated with increased Tyr15 phosphorylation of CDK1, reduced CDC25B, reduced formation of cyclin A/CDK1 complex (demonstrated by co-immunoprecipitation), and activation of p53 (Ser15 phosphorylation) with increased p21 expression. EGF stabilizes IFI27 protein by prolonging its half-life. siRNA knockdown and overexpression in keratinocytes; MTT assay; flow cytometry; co-immunoprecipitation of cyclin A/CDK1; Western blot; in vivo topical siRNA in imiquimod-induced psoriasis mouse model Cell proliferation Medium 25664647
2016 Full-length ISG12A/IFI27 protein sensitizes cells to TNF-alpha- and gossypol-induced apoptosis; shorter ISG12A variants directly induce apoptosis in HEK293 cells. ISG12A co-immunoprecipitates with Bcl-2, and apoptotic effects are reduced in Bcl-2-expressing cells. Two putative BH3-like motifs in the ISG12 motif are implicated. Full-length ISG12A forms homodimers suggesting a role in pore formation. ISG12A, its variants, and ISG12B localize to mitochondria. Overexpression in HEK293 cells; BH3 mutagenesis; co-immunoprecipitation with Bcl-2; mitochondrial localization by subcellular fractionation/imaging; apoptosis assays in Bcl-2-expressing cells Biology of the cell High 27673746
2018 Ifi27 protein localizes to mitochondria in adipocytes. Knockdown of Ifi27 reduces expression of TCA cycle key enzymes, electron transport chain subunits, and Ucp1 in brown and beige adipocytes, and blocks beta3-adrenergic agonist-induced browning of subcutaneous white fat. Ifi27 expression is induced by beta3-adrenergic activation in adipose tissue. Mitochondrial localization by imaging/fractionation; siRNA knockdown; overexpression in brown adipocytes; in vivo beta3-adrenergic agonist treatment; RT-qPCR and Western blot for TCA/ETC/Ucp1 markers Biochemical and biophysical research communications Medium 29730295
2020 IFI27/ISG12 interacts with estrogen receptor alpha (ERα) and facilitates the interaction between ERα and CRM1/XPO1, promoting nuclear export of ERα to the cytoplasm, thereby attenuating ERα transactivation activity and expression of ERα-dependent genes. IFI27 overexpression reduces MCF-7 cell proliferation and migration. Co-immunoprecipitation of IFI27 with ERα and CRM1/XPO1; overexpression in MCF-7 breast cancer cells; reporter assay for ERα transactivation; 2D/3D proliferation assays; wound-healing migration assay Frontiers in endocrinology Medium 33117284
2021 ATF3 transcription factor binds the promoter regions of IFI27 (and IFI6) as shown by ChIP assay, and negatively regulates IFI27 expression. Loss of ATF3 upregulates IFI27 and promotes TSCC cell growth and migration; IFI27 knockdown blocks the growth/migration induced by ATF3 deletion, and IFI27 overexpression counteracts ATF3 overexpression-mediated inhibition. ChIP assay; RNA-seq; gain- and loss-of-function of ATF3 in four TSCC cell lines; siRNA knockdown of IFI27; in vivo mouse model PLoS genetics High 33539340
2022 PABPC1 interacts with eIF4G to increase the stability of IFI27 mRNA by competing with RNA exosomes, activating the IFN/IFI27 signaling pathway to enhance ESCC cell proliferation and invasion. Co-immunoprecipitation of PABPC1 with eIF4G; mRNA stability assays; PABPC1 overexpression/knockdown in ESCC cells; in vitro and in vivo proliferation/invasion assays Journal of experimental & clinical cancer research Medium 35346324
2023 IFI27 counteracts innate immune responses triggered by cytoplasmic RNA recognition after influenza A virus, SARS-CoV-2, Sendai virus infections, and dsRNA transfection. IFI27 interacts with nucleic acids and with RIG-I (most likely RNA-mediated), and this interaction impairs RIG-I activation. IFI27 has a positive effect on IAV and SARS-CoV-2 replication in vitro and in vivo. Co-immunoprecipitation of IFI27 with RIG-I; IFI27 knockdown and overexpression cells; viral infection models (IAV, SARS-CoV-2, SeV); dsRNA transfection; in vivo experiments; innate immune response assays Frontiers in microbiology Medium 37187533
2023 IFI27 is required for cristae morphogenesis and sustains succinate dehydrogenase (SDH) function and fatty acid oxidation in brown adipocytes. IFI27 physically binds SDHB and HADHA (identified by interaction mapping). IFI27 links SDHB to chaperone TRAP1, shielding SDHB from oxidative damage-triggered degradation. IFI27 also increases HADHA catalytic activity in beta-oxidation. Ifi27-knockout brown fat shows reduced SDH levels, impaired fatty acid oxidation, impaired oxygen consumption and defective thermogenesis. IFI27 protein interaction mapping; co-immunoprecipitation of IFI27 with SDHB, HADHA, TRAP1; Ifi27-knockout mice; oxygen consumption assay; beta-oxidation assay; cold challenge thermogenesis model Advanced science High 37544897
2024 IFI27 co-immunoprecipitates with MDA5, with this interaction likely mediated by RNAs. IFI27 inhibits MDA5 oligomerization and activation, counteracting innate immune responses after SARS-CoV-2 infection or poly(I:C) transfection. IFI27 competes with MDA5 for poly(I:C) binding, providing a mechanism for its inhibition of MDA5 activation. Co-immunoprecipitation of IFI27 with MDA5; IFI27 overexpression, knockout, and knockdown cells; MDA5 oligomerization assay; poly(I:C) binding competition assay; SARS-CoV-2 infection model Frontiers in cellular and infection microbiology Medium 39431052
2024 IFI27 inhibits HIV-1 replication by degrading viral Gag proteins (p24 and p55) via the ubiquitin-proteasome pathway. The NPM-IFI27-37-115 domain interacts with the p24-N domain; the NPM-IFI27-76-122 domain is associated with K48 ubiquitin recruitment. SKP2 is identified as the probable E3 ubiquitin ligase mediating IFI27-induced p24/p55 ubiquitination and degradation. Human IFI27 shows a similar mechanism. Co-immunoprecipitation of IFI27 with p24/p55; ubiquitin-proteasome pathway assay; domain mapping; SKP2 identification as E3 ligase; IFI27 overexpression and knockdown; HIV-1 replication assay Journal of virology High 39475279
2025 IFI27 suppresses HBV transcription by promoting ubiquitination-dependent proteasomal degradation of C/EBPα (a cellular transcription factor critical for HBV RNA transcription) in the cytoplasm. The E3 ubiquitin ligase SKP2 mediates IFI27-induced C/EBPα ubiquitination and degradation; SKP2 knockdown abrogates IFI27's antiviral activity. IFI27 is predominantly localized in the cytoplasm. IFI27 knockdown diminishes IFN-alpha antiviral effect on HBV. IFI27 overexpression and knockdown in HBV-transfected and -infected cells (HepG2-NTCP, primary human hepatocytes); ubiquitination assay; SKP2 knockdown rescue experiment; HBV RNA transcription assay; RNA-seq; subcellular localization Journal of virology High 41081508
2025 IFI27 interacts with PACT (PRKRA) and with PKR, where the IFI27-PACT interaction is likely mediated by dsRNAs or RNAs containing duplex regions, and the IFI27-PKR interaction is PACT-dependent. IFI27 potentiates PKR activity, leading to decreased protein translation, increased eIF2alpha phosphorylation, and increased stress granule formation. This function is demonstrated in cells infected with SARS-CoV-2, VSV, and after poly(I:C) transfection. Co-immunoprecipitation of IFI27 with PACT and PKR; IFI27 knockdown, knockout, and overexpression cells; eIF2alpha phosphorylation assay; stress granule imaging; protein translation assay; viral infection (SARS-CoV-2, VSV) PLoS pathogens Medium 40522981
2022 USP18 deubiquitinates IFI27 and prevents its degradation (demonstrated by Co-IP). HOXA5 is a transcription factor that activates USP18 transcription by binding to its promoter (validated by ChIP and dual-luciferase assay), thereby stabilizing IFI27 and promoting ESCC cell malignant progression and cisplatin resistance. Co-immunoprecipitation of USP18 with IFI27; ChIP and dual-luciferase assay for HOXA5 binding USP18 promoter; IFI27 knockdown; ubiquitination/deubiquitination assays; cell viability, proliferation, apoptosis, invasion assays Thoracic cancer Medium 39993992
2025 METTL3-mediated m6A methylation of IFI27 mRNA stabilizes the transcript; the m6A reader IGF2BP2 also affects IFI27 mRNA m6A methylation and expression. METTL3 depletion reduces IFI27 expression; IFI27 re-expression rescues the effects of METTL3 deficiency on ESCC cell behaviors. Methylated RNA immunoprecipitation (MeRIP); RIP assay; mRNA stability analysis; METTL3 and IGF2BP2 knockdown; IFI27 re-expression rescue; xenograft assay Journal of biochemical and molecular toxicology Medium 39987518
2022 miR-942-5p suppresses IFI27 translation by targeting the 3'-UTR of the IFI27 gene. IFI27 overexpression increases apoptosis and decreases Cryptosporidium parvum burden in HCT-8 cells via TRAIL and caspase-8 pathway. C. parvum induces downregulation of IFI27 via upregulation of miR-942-5p. 3'-UTR reporter assay; miR-942-5p overexpression/suppression; IFI27 overexpression/knockdown; flow cytometry (apoptosis); Western blot of TRAIL and caspase-8; C. parvum burden quantification Parasites & vectors Medium 35974384
2024 ADAMTS16 upregulates IFI27 protein through the NF-κB pathway, with a binding site between the NF-κB subunit P65 and the IFI27 promoter identified by dual-luciferase reporter assay. IFI27 knockdown reverses the promoting effects of ADAMTS16 on gastric cancer cell invasion, migration, and proliferation. Co-immunoprecipitation; immunofluorescence; western blot; dual-luciferase reporter assay for P65-IFI27 promoter binding; IFI27 knockdown rescue; in vitro and in vivo functional assays International journal of molecular sciences Medium 36232317
2024 In situ single-cell spatial transcriptomics identified M1 macrophages as having elevated IFI27 expression in areas of SARS-CoV-2 infection, providing direct cell-type-level localization of IFI27 expression during infection. In situ single-cell resolved spatial transcriptomics during SARS-CoV-2 infection EBioMedicine Low 38377798

Source papers

Stage 0 corpus · 56 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2017 A novel immune biomarker IFI27 discriminates between influenza and bacteria in patients with suspected respiratory infection. The European respiratory journal 106 28619954
2010 Emerging roles of FAM14 family members (G1P3/ISG 6-16 and ISG12/IFI27) in innate immunity and cancer. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 105 20939681
2004 Interferon alpha-inducible protein 27 (IFI27) is upregulated in psoriatic skin and certain epithelial cancers. The Journal of investigative dermatology 91 15086558
2022 PABPC1-induced stabilization of IFI27 mRNA promotes angiogenesis and malignant progression in esophageal squamous cell carcinoma through exosomal miRNA-21-5p. Journal of experimental & clinical cancer research : CR 80 35346324
2004 Identification of a novel gene family that includes the interferon-inducible human genes 6-16 and ISG12. BMC genomics 77 14728724
2022 ScRNA-seq expression of IFI27 and APOC2 identifies four alveolar macrophage superclusters in healthy BALF. Life science alliance 59 35820705
2011 Whole-blood transcriptional profiling of interferon-inducible genes identifies highly upregulated IFI27 in primary myelofibrosis. European journal of haematology 56 21447007
2023 IFI27 transcription is an early predictor for COVID-19 outcomes, a multi-cohort observational study. Frontiers in immunology 55 36685600
2016 Apoptotic properties of the type 1 interferon induced family of human mitochondrial membrane ISG12 proteins. Biology of the cell 55 27673746
2023 The IFN-stimulated gene IFI27 counteracts innate immune responses after viral infections by interfering with RIG-I signaling. Frontiers in microbiology 50 37187533
2015 IFI27, a novel epidermal growth factor-stabilized protein, is functionally involved in proliferation and cell cycling of human epidermal keratinocytes. Cell proliferation 44 25664647
2000 The interferon-induced gene ISG12 is regulated by various cytokines as the gene 6-16 in human cell lines. Cytokine 44 10704250
2001 The interferon alpha induced protein ISG12 is localized to the nuclear membrane. European journal of biochemistry 37 11722583
2018 Knockdown of IFI27 inhibits cell proliferation and invasion in oral squamous cell carcinoma. World journal of surgical oncology 31 29580248
2021 IFI27 may predict and evaluate the severity of respiratory syncytial virus infection in preterm infants. Hereditas 27 33388093
2021 ATF3 downmodulates its new targets IFI6 and IFI27 to suppress the growth and migration of tongue squamous cell carcinoma cells. PLoS genetics 26 33539340
2013 ISG12 is a critical modulator of innate immune responses in murine models of sepsis. Immunobiology 25 23747037
2013 The association of IFI27 expression and fatigue intensification during localized radiation therapy: implication of a para-inflammatory bystander response. International journal of molecular sciences 25 23959120
2013 Regulation of Interferon-stimulated Gene (ISG)12, ISG15, and MX1 and MX2 by Conceptus Interferons (IFNTs) in Bovine Uterine Epithelial Cells. Asian-Australasian journal of animal sciences 25 25049852
2022 IFI27 is a potential therapeutic target for HIV infection. Annals of medicine 23 35068272
2020 IFI27/ISG12 Downregulates Estrogen Receptor α Transactivation by Facilitating Its Interaction With CRM1/XPO1 in Breast Cancer Cells. Frontiers in endocrinology 23 33117284
2018 Ifi27 is indispensable for mitochondrial function and browning in adipocytes. Biochemical and biophysical research communications 23 29730295
2023 IFI27 Integrates Succinate and Fatty Acid Oxidation to Promote Adipocyte Thermogenic Adaption. Advanced science (Weinheim, Baden-Wurttemberg, Germany) 21 37544897
2016 IFI27 Is a Useful Genetic Marker for Diagnosis of Immunoglobulin A Nephropathy and Membranous Nephropathy Using Peripheral Blood. PloS one 21 27100186
2022 The Novel Protein ADAMTS16 Promotes Gastric Carcinogenesis by Targeting IFI27 through the NF-κb Signaling Pathway. International journal of molecular sciences 20 36232317
2023 Integrative bulk and single-cell transcriptome profiling analysis reveals IFI27 as a novel interferon-stimulated gene in dengue. Journal of medical virology 18 36971141
2018 High level expression of ISG12(1) promotes cell apoptosis via mitochondrial-dependent pathway and so as to hinder Newcastle disease virus replication. Veterinary microbiology 16 30593361
2025 Interferon-stimulated gene IFI27 as a multifaceted candidate target in precision medicine. Trends in immunology 15 40044530
2023 ATM Inhibition-Induced ISG15/IFI27/OASL Is Correlated with Immunotherapy Response and Inflamed Immunophenotype. Cells 15 37174688
2022 MiR-942-5p targeting the IFI27 gene regulates HCT-8 cell apoptosis via a TRAIL-dependent pathway during the early phase of Cryptosporidium parvum infection. Parasites & vectors 15 35974384
2020 IFI27 as a potential indicator for severe Enterovirus 71-infected hand foot and mouth disease. Virus research 13 32866535
2015 The Impact of IL28B Genotype and Liver Fibrosis on the Hepatic Expression of IP10, IFI27, ISG15, and MX1 and Their Association with Treatment Outcomes in Patients with Chronic Hepatitis C. PloS one 12 26115415
2024 Epstein-Barr virus lytic gene BNRF1 promotes B-cell lymphomagenesis via IFI27 upregulation. PLoS pathogens 11 38300891
2013 Brahma‑related gene 1-associated expression of 9-27 and IFI-27 is involved in acquired cisplatin resistance of gastric cancer cells. Molecular medicine reports 10 23836109
2023 Integrated Bioinformatics and Validation Reveal IFI27 and Its Related Molecules as Potential Identifying Genes in Liver Cirrhosis. Biomolecules 9 38275754
2012 Interferon-inducible genes, TNF-related apoptosis-inducing ligand (TRAIL) and interferon inducible protein 27 (IFI27) are negatively regulated in leiomyomas: implications for a role of the interferon pathway in leiomyoma development. Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology 9 22320196
2023 Whole-genome sequencing identifies interferon-induced protein IFI6/IFI27-like as a strong candidate gene for VNN resistance in European sea bass. Genetics, selection, evolution : GSE 7 37143017
2024 DDX60 Promotes Migration and Invasion of Head and Neck Squamous Cell Carcinoma Cell through the NF-κB/IFI27 Signaling Pathway. Frontiers in bioscience (Landmark edition) 6 38287816
2024 In situ single-cell profiling sheds light on IFI27 localisation during SARS-CoV-2 infection. EBioMedicine 6 38377798
2024 IFI27 inhibits HIV-1 replication by degrading Gag protein through the ubiquitin-proteasome pathway. Journal of virology 6 39475279
2022 Chicken ISG12(2) attenuates Newcastle disease virus and enhances the efficiency of Newcastle disease vaccine via activating immune pathways. Transboundary and emerging diseases 6 34904395
2003 A nine-nucleotide deletion and splice variation in the coding region of the interferon induced ISG12 gene. Biochimica et biophysica acta 6 12878323
2025 HOXA5 Derives the Malignant Progression and Cisplatin Resistance of Esophageal Squamous Cell Carcinoma by Regulating USP18-Mediated IFI27 Deubiquitination. Thoracic cancer 4 39993992
2025 Interferon alpha-inducible protein 27 (IFI27) inhibits hepatitis B virus (HBV) transcription through downregulating cellular transcription factor C/EBPα. Journal of virology 4 41081508
2024 The IFN-induced protein IFI27 binds MDA5 and counteracts its activation after SARS-CoV-2 infection. Frontiers in cellular and infection microbiology 4 39431052
2024 IFI27 enhances bladder cancer immunotherapy response by modulating regulatory T cell enrichment. Journal of Cancer 4 39668835
2021 A IFI27 gene contributes to ER-stress mediated apoptosis and benefits for white spot syndrome virus infection in Litopenaeus vannamei. Fish & shellfish immunology 4 34838985
2025 IFI27, a potential candidate molecular marker for primary Sjogren's syndrome. Clinical rheumatology 3 40146445
2025 METTL3-Mediated m6A Methylation Stabilizes IFI27 to Drive Esophageal Squamous Cell Carcinoma Progression Through an IGF2BP2-Dependent Mechanism. Journal of biochemical and molecular toxicology 2 39987518
2024 Effects of Genetic Polymorphism in the IFI27 Gene on Milk Fat Traits and Relevance to Lipid Metabolism in Bovine Mammary Epithelial Cells. Animals : an open access journal from MDPI 1 39595334
2026 GJB2 c.109G > A mutation activating IFI27-mediated mitochondrial apoptosis pathway leading to hereditary non-syndromic hearing loss. Scientific reports 0 41588186
2026 Automated whole-blood detection of IFI27 and OAS1 mRNA for distinguishing active systemic lupus erythematosus. Rheumatology (Oxford, England) 0 41936093
2026 IFI27-mediated regulation of regulatory T cells aggravates lung injury in sepsis via IL-10/STAT3 signaling. Frontiers in immunology 0 42099627
2026 IFI27 modulates the immune response in childhood neuropsychiatric systemic lupus erythematosus by regulating CD8+ T cell function via the IFN-I signaling pathway. Cytokine 0 42105695
2025 IFN alpha inducible protein 27 (IFI27) acts as a positive regulator of PACT-dependent PKR activation after RNA virus infections. PLoS pathogens 0 40522981
2025 Evaluating the InSignia IFI27 expression assay for detecting viral respiratory infection compared to a traditional gene normalisation assay. Scientific reports 0 40593901

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