Affinage

Showing H2BC15 — HIST1H2BN is a alias.

H2BC15

Histone H2B type 1-N · UniProt Q99877

Length
126 aa
Mass
13.9 kDa
Annotated
2026-06-10
45 papers in source corpus 2 papers cited in narrative 2 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 1/1 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

H2BC15 (HIST1H2BN) is a histone H2B family member implicated in oncogenic signaling in prostate cancer, where its knockdown inhibits cell proliferation, migration, and the EMT phenotype by diminishing the expression and DNA-binding activity of NF-κB p65 and thereby reducing downstream MMP2 and MMP9 expression (PMID:34537918). The direct molecular mechanism linking the histone to NF-κB activity is not characterized in the available corpus. Separately, histone H2B in mammalian brain carries an Asp25 D-isoAsp modification enriched relative to other tissues, but this modification has not been mapped specifically to the H2BC15 paralog (PMID:36717395). Beyond these two Low-confidence observations, no further mechanistic detail has been characterized in the available corpus.

Mechanistic history

Synthesis pass · year-by-year structured walk · 2 steps
  1. 2021 Low

    Whether this H2B paralog contributes to cancer phenotypes was unknown; knockdown linked it to an NF-κB–MMP–EMT axis, framing it as a candidate oncogenic regulator.

    Evidence siRNA knockdown in DU145 and PC3 prostate cancer lines with proliferation, migration, EMT marker, and NF-κB p65 binding-activity assays

    PMID:34537918

    Open questions at the time
    • Mechanism inferred from correlation; no reconstitution, mutagenesis, or rescue establishing direct causality
    • How a histone protein modulates NF-κB p65 expression and DNA binding is undefined
    • Single lab, single study, restricted to two cell lines
  2. 2023 Low

    Whether H2B carries tissue-specific covalent modifications was addressed by detecting a brain-enriched Asp25 D-isoAsp modification, raising the possibility of paralog-specific regulation.

    Evidence Polyclonal antibody specificity validation and immunodetection across brain, liver, thymus, and HeLa cells

    PMID:36717395

    Open questions at the time
    • Antibody-based detection only; not mapped specifically to the H2BC15 paralog
    • No functional consequence of the modification demonstrated
    • Single method and single lab

Open questions

Synthesis pass · forward-looking unresolved questions
  • The direct molecular mechanism by which H2BC15 influences NF-κB signaling, and whether its chromatin/histone function connects to that signaling, remains unresolved.
  • No structural or biochemical link between the histone and NF-κB established
  • No demonstration that H2BC15 deposition or modification drives the cancer phenotype

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
No controlled-vocabulary terms were assigned to this entry.

Evidence

Reading pass · 2 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2021 HIST1H2BN (H2BC15) knockdown in prostate cancer cells (DU145 and PC3) inhibited cell proliferation, migration, and EMT phenotype. Mechanistically, downregulating HIST1H2BN diminished the expression and DNA-binding activity of NF-κB p65, which in turn reduced expression of MMP2 and MMP9, establishing a HIST1H2BN–NF-κB–EMT regulatory axis in oncogenesis. siRNA knockdown in prostate cancer cell lines, proliferation and migration assays, EMT marker assessment, NF-κB p65 expression and binding activity assays Genes & genomics Low 34537918
2023 Approximately 12% of histone H2B molecules in mammalian brain contain a post-translational modification at Asp25, present as the D-enantiomer (D-isoAsp-25) linked to Gly26 via the side-chain carboxyl. This modification is enriched in brain relative to liver, thymus, and HeLa cells, as demonstrated by a highly selective polyclonal antibody. Polyclonal antibody characterization with specificity validation; tissue fractionation and immunodetection across brain, liver, thymus, and HeLa cells Amino acids Low 36717395

Source papers

Stage 0 corpus · 45 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1996 CDP/cut is the DNA-binding subunit of histone gene transcription factor HiNF-D: a mechanism for gene regulation at the G1/S phase cell cycle transition point independent of transcription factor E2F. Proceedings of the National Academy of Sciences of the United States of America 115 8876167
1974 Antigen-specific thymus cell factors in the genetic control of the immune response to poly-(tyrosyl, glutamyl)-poly-D, L-alanyl--poly-lysyl. The Journal of experimental medicine 115 4136175
1983 Early development of the T cell repertoire. In vivo treatment of neonatal mice with anti-Ia antibodies interferes with differentiation of I-restricted T cells but not K/D-restricted T cells. The Journal of experimental medicine 92 6222135
1986 Mercuric chloride-, gold sodium thiomalate-, and D-penicillamine-induced antinuclear antibodies in mice. Toxicology and applied pharmacology 90 3097874
2004 Effects of complement factor D deficiency on the renal disease of MRL/lpr mice. Kidney international 87 14675043
1979 Regulation of T-cell-mediated lympholysis by the murine major histocompatibility complex. I. Preferential in vitro responses to trinitrophenyl-modified self K- and D-coded gene products in parental and F1 hybrid mouse strains. The Journal of experimental medicine 66 109568
1994 MHC class I gene(s) in the D/L region but not the TNF-alpha gene determines development of toxoplasmic encephalitis in mice. Journal of immunology (Baltimore, Md. : 1950) 59 7963536
2011 Shot-gun proteomic analysis of mitochondrial D-loop DNA binding proteins: identification of mitochondrial histones. Molecular bioSystems 55 21359316
1994 Cathepsin D, but not cathepsin B, releases T cell stimulatory fragments from lysozyme that are functional in the context of multiple murine class II MHC molecules. European journal of immunology 55 8088334
1983 Genetic regulation of the immune response to hepatitis B surface antigen (HBsAg). II. Qualitative characteristics of the humoral immune response to the a, d, and y determinants of HBsAg. Journal of immunology (Baltimore, Md. : 1950) 42 6185581
1981 Cross-reactions between mouse Ia and human HLA-D/DR antigens analyzed with monoclonal alloantibodies. Journal of immunology (Baltimore, Md. : 1950) 42 6785353
2013 Transcription-coupled eviction of histones H2A/H2B governs V(D)J recombination. The EMBO journal 39 23463099
1983 Differential promotion and suppression of Z leads to B transitions in poly[d(G-C)] by histone subclasses, polyamino acids and polyamines. The EMBO journal 36 6641700
1990 D-penicillamine-induced lupus erythematosus. Arthritis and rheumatism 30 2222540
1993 Low codon bias and high rates of synonymous substitution in Drosophila hydei and D. melanogaster histone genes. Molecular biology and evolution 26 8487638
1993 In vitro and in vivo comparative studies on immunosuppressive properties of cyclosporines A, C, D and metabolites M1, M17 and M21. Immunopharmacology and immunotoxicology 25 8349948
2005 Identification of the immunodominant HY H2-D(k) epitope and evaluation of the role of direct and indirect antigen presentation in HY responses. Journal of immunology (Baltimore, Md. : 1950) 23 16301625
1989 Structural relationships among the H-2 D-regions of murine MHC haplotypes. Journal of immunology (Baltimore, Md. : 1950) 23 2909618
1978 Ia versus K/D antigens in immunological enhancement of tumor allografts. Transplantation 22 635985
1982 Murine alloantigen acquisition by schistosomula of Schistosoma mansoni: further evidence for the presence of K, D, and I region gene products on the tegumental surface. Parasite immunology 20 6185906
2021 RNF20 and RNF40 regulate vitamin D receptor-dependent signaling in inflammatory bowel disease. Cell death and differentiation 19 34088983
1987 Analysis of the D-region products of H-2q using monoclonal antibodies reveals the expression of a new class I-like molecule. Immunogenetics 14 3102360
1991 Solution conformation and dynamics of the octadeoxy-nucleotide d(CACTAGTG)2: a multinuclear n.m.r. relaxation study. Carbohydrate research 12 1667855
2019 Actinomycin D-Activated RNase L Promotes H2A.X/H2B-Mediated DNA Damage and Apoptosis in Lung Cancer Cells. Frontiers in oncology 10 31750234
1998 Novel diversity in Th1, Th2 type differentiation of hemagglutinin-specific T cell clones elicited by natural influenza virus infection in three major haplotypes (H-2b,d,k). Journal of immunology (Baltimore, Md. : 1950) 9 9686567
2021 HIST1H2BN induced cell proliferation and EMT phenotype in prostate cancer via NF-κB signal pathway. Genes & genomics 8 34537918
2000 H-2(d) mice born to and reared by HBeAg-transgenic mothers do not develop T cell tolerance toward the hepatitis B virus core gene products. Virology 7 10891417
2025 Multifaceted roles of H2B mono-ubiquitylation in D-loop metabolism during homologous recombination repair. Nucleic acids research 6 39945322
2004 An organizational model of transcription factor binding sites for a histone promoter in D. melanogaster. In silico biology 6 15752071
1982 The head protein D of bacterial virus lambda is related to eukaryotic chromosomal proteins. The EMBO journal 6 6233140
2017 Low expression of a Ddm7/Ldm7-hybrid mutant (D/Ldm7) in the novel haplotype H-2nc identified in atopic dermatitis model NC/Nga mice. Genes and immunity 5 29282355
1977 Intra-H-2 recombination in H-2b/H-2t1 heterozygotes in the mouse I. Four cases of crossing over between the S And D regions. Tissue antigens 5 557247
1983 Target determinants for F1 hybrid anti-parental H-2d cell-mediated lympholysis: self antigens controlled by the D end. Journal of immunology (Baltimore, Md. : 1950) 4 6187857
1983 Regulation of the hapten-specific T cell response. I. Preferential induction of hyporesponsiveness to the D-end of the major histocompatibility complex in the hapten-specific cytotoxic T cell response. Journal of immunology (Baltimore, Md. : 1950) 4 6195256
1983 Rapidly phosphorylated histone-like proteins are modulated in the course of transcription block by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole. European journal of cell biology 4 6641743
2016 Evolution of GC content in the histone gene repeating units from Drosophila lutescens, D. takahashii and D. pseudoobscura. Genes & genetic systems 3 27021916
2002 Differences in antigen processing with haplotype-mismatched MHC class II heterodimers: Aalpha(d)Abeta(b) heterodimers participate in early endosomal processing. European journal of immunology 3 12355424
1997 Lack of F1 anti-parental resistance in H-2b/d F1 hybrids devoid of beta2-microglobulin. European journal of immunology 3 9022038
1997 Immunogenic properties of synthetic fragments of Sm-D protein in normal and lupus mice. Lupus 3 9364425
2024 Genome-wide analysis of the biophysical properties of chromatin and nuclear proteins in living cells with Hi-D. Nature protocols 2 39198619
2021 MST1 mediates the N-methyl-D-aspartate-induced excitotoxicity in mouse cortical neurons. Cellular and molecular life sciences : CMLS 2 34967918
2024 Validation of novel conditional ligands and large-scale detection of antigen-specific T cells for H-2Dd and H-2Kd. Scientific reports 0 38811654
2024 Multifaceted roles of H2B mono-ubiquitylation in D-loop metabolism during homologous recombination repair. bioRxiv : the preprint server for biology 0 39314463
2023 Characterization of a polyclonal antibody that is highly selective for the D-isoAsp-25 variant of mammalian histone H2B. Amino acids 0 36717395
1983 Suppressive effects of soluble histocompatibility antigens on the in vitro generation of cytotoxic T cells to D-end alloantigens. Cellular immunology 0 6222790

Missed literature

Know a paper Affinage missed for H2BC15? Flag it for the maintainers and the community.

No submissions yet.