Affinage

Showing H2AC14 — HIST1H2AJ is a alias.

H2AC14

Histone H2A type 1-J · UniProt Q99878

Length
128 aa
Mass
13.9 kDa
Annotated
2026-06-10
12 papers in source corpus 1 papers cited in narrative 1 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 2/3 claims corpus-supported (67%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

H2AC14 encodes a replication-coupled histone H2A variant that is stably incorporated into chromatin and behaves as a functional component of the nucleosome (PMID:7957682). Expressed in HeLa cells, the protein (alias H2A.E) accumulates to roughly 10% of total H2A, carries an extended C-terminus, and exhibits chromatin stability and soluble-versus-nuclear partitioning indistinguishable from canonical H2A species; its mRNA levels are coupled to rates of DNA and protein synthesis, consistent with replication-linked regulation (PMID:7957682). Beyond (PMID:7957682), no further mechanistic detail — partners, post-translational regulation, or distinct functional role relative to other H2A isoforms — has been characterized in the available corpus.

Mechanistic history

Synthesis pass · year-by-year structured walk · 1 step
  1. 1994 Medium

    Established that the H2AC14 gene product is a bona fide replication-coupled histone rather than a non-functional variant, by showing it is incorporated into chromatin and behaves like canonical H2A.

    Evidence Stable transfection of HeLa cells with a modified histone gene construct, two-dimensional AUT-AUC gel electrophoresis to resolve the protein, soluble/nuclear fractionation, and mRNA stability assays correlated with DNA synthesis

    PMID:7957682

    Open questions at the time
    • Whether the extended C-terminus confers any function distinct from canonical H2A is untested
    • No interacting partners, modifying enzymes, or genomic distribution determined
    • Functional consequences of incorporation (transcription, chromatin compaction) not assessed

Open questions

Synthesis pass · forward-looking unresolved questions
  • Whether H2AC14 has any specialized chromatin function distinguishing it from other replication-dependent H2A isoforms remains unknown.
  • No structural or genomic-occupancy data
  • No defined biological process beyond generic nucleosome assembly
  • No disease or phenotypic association characterized in the corpus

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0005198 structural molecule activity 1
Localization
GO:0000228 nuclear chromosome 1 GO:0005634 nucleus 1
Pathway
R-HSA-4839726 Chromatin organization 1
Complex memberships
nucleosome

Evidence

Reading pass · 1 per-paper finding extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1994 A marked H2A histone protein (H2A.E, an alias for H2AC14) with an extended C-terminus (8 additional amino acids including 3 methionines) was incorporated into chromatin of HeLa cells at ~10% of total H2A. Its mRNA levels were coupled to rates of DNA and protein synthesis (replication-linked regulation), and its stability in chromatin and partitioning between soluble and nuclear fractions were indistinguishable from other histone species, demonstrating that H2AC14/H2A.E behaves as a functional replication-coupled histone. Stable transfection of HeLa cells with modified histone gene construct; two-dimensional AUT-AUC gel electrophoresis to resolve the novel protein; fractionation into soluble and nuclear compartments; mRNA stability assays correlated with DNA synthesis rates Experimental cell research Medium 7957682

Source papers

Stage 0 corpus · 12 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2016 Identification and validation of candidate epigenetic biomarkers in lung adenocarcinoma. Scientific reports 58 27782156
2016 Investigating core genetic-and-epigenetic cell cycle networks for stemness and carcinogenic mechanisms, and cancer drug design using big database mining and genome-wide next-generation sequencing data. Cell cycle (Georgetown, Tex.) 29 27295129
2003 HLA and H2 class II transgenic mouse models to study susceptibility and protection in autoimmune thyroid disease. Autoimmunity 18 14669947
2018 Epigenetic basis of hepatocellular carcinoma: A network-based integrative meta-analysis. World journal of hepatology 13 29399289
2023 Transcriptomic identification of genes expressed in invasive S. aureus diabetic foot ulcer infection. Frontiers in cellular and infection microbiology 8 37434783
2023 Characteristics of ABCC4 and ABCG2 High Expression Subpopulations in CRC-A New Opportunity to Predict Therapy Response. Cancers 4 38067326
2025 Initial Insights into the NET-Associated ceRNA Network in Pulpitis: Transcriptomic and Functional Exploration. International dental journal 3 40902508
2001 Characterization of a novel H2A(-)E+ transgenic model susceptible to heterologous but not self thyroglobulin in autoimmune thyroiditis: thyroiditis transfer with Vbeta8+ T cells. Cellular immunology 3 11716530
1994 Expression of a marked H2A histone protein in mammalian cells. Experimental cell research 1 7957682
2026 Identification and validation of palmitoylation-associated biomarkers in major depressive disorder. Scientific reports 0 42225708
2025 Identification of novel H2A histone variants across diverse clades of algae. Genome biology 0 40988048
2023 SMARCB1-Retained and SMARCB1-Deficient SNUC are Genetically Distinct: A Pilot Study Using RNA Sequencing. Journal of neurological surgery. Part B, Skull base 0 38966291

Missed literature

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