Affinage

Showing DLGAP1 — GKAP is a alias.

DLGAP1

Disks large-associated protein 1 · UniProt O14490

Length
977 aa
Mass
108.9 kDa
Annotated
2026-06-09
67 papers in source corpus 22 papers cited in narrative 22 extracted findings
Cross-family judge faithfulness: 7/7 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

DLGAP1 (GKAP/SAPAP1) is a core scaffolding protein of the postsynaptic density (PSD) that physically bridges the membrane receptor–MAGUK layer to the cytoskeleton-associated Shank–Homer layer (PMID:9024696, PMID:10433268). Through its central region it directly binds the guanylate kinase-like (GK) domain of all four PSD-95 family MAGUKs (PSD-95, SAP97, SAP98, SAP102), coclustering with these scaffolds and NMDA receptors at excitatory synapses (PMID:9024696, PMID:9286858), and through its intrinsically disordered C-terminus it binds the PDZ domain of Shank/ProSAP proteins to assemble a ternary Shank/GKAP/PSD-95 complex (PMID:10433268, PMID:10527873, PMID:41191219). GKAP forms a static, immobile core of the PSD whose synaptic targeting is mediated by its N-terminus independently of PSD-95, and which is required to localize Shank (PMID:12581155); acute elimination of GKAP reduces postsynaptic scaffold size, defining a direct structural role in PSD maintenance [PMID:bio_10.1101_2024.10.20.619267]. Functionally, GKAP potentiates NMDA receptor–PSD-95 channel activity (PMID:10570927), and it self-associates into large hetero-oligomers with the dynein light chain DLC2/DYNLL2 (a defined GKAP–LC8 hexamer of two GKAP and two LC8 dimers) that stabilize PSD scaffolds and enhance NMDA receptor currents in dendritic spines (PMID:22328512, PMID:24938595, PMID:40843979). Its synaptic abundance is bidirectionally controlled by phosphorylation: CaMKIIα phosphorylation of Ser54 drives TRIM3-mediated ubiquitination and proteasomal degradation, while CaMKIIβ phosphorylation of Ser340/Ser384 uncouples GKAP from the myosin Va motor to promote synaptic accumulation, governing homeostatic synaptic scaling and PSD remodeling (PMID:20352094, PMID:23143515), and CDK5 phosphorylates GKAP to trigger its degradation and actin collapse in response to amyloid-β (PMID:21829588). Beyond the synapse, GKAP links Dlg1 to dynein to control microtubule dynamics and centrosome positioning during directed cell migration downstream of Cdc42 (PMID:21041448). Dlgap1 knockout mice show disrupted PSD protein complex organization and selective deficits in sociability, establishing the scaffold as necessary for normal social behavior (PMID:29396406).

Mechanistic history

Synthesis pass · year-by-year structured walk · 12 steps
  1. 1997 High

    Established GKAP/DLGAP1 as a direct binding partner of the PSD-95 MAGUK family, answering how an adaptor could be recruited to the NMDA receptor–MAGUK complex.

    Evidence Yeast two-hybrid, co-IP from rat brain, and heterologous coexpression with PSD-95 and NMDA receptors

    PMID:9024696 PMID:9286858

    Open questions at the time
    • Did not identify the downstream cytoskeletal coupling
    • Binding mapped to the GK domain but functional consequence at synapses untested
  2. 1999 High

    Defined the GKAP C-terminus as the Shank/ProSAP PDZ ligand, showing GKAP bridges PSD-95 to the Shank layer and cytoskeleton.

    Evidence Yeast two-hybrid, co-IP from rat brain, heterologous ternary-complex reconstitution and splice-variant disruption of Shank targeting

    PMID:10433268 PMID:10527873

    Open questions at the time
    • Structural basis of the disordered C-terminus binding resolved only later
    • Stoichiometry of the ternary complex unquantified
  3. 1999 Medium

    Showed GKAP is not merely structural but quantitatively potentiates NMDA receptor channel activity via PSD-95.

    Evidence Electrophysiological channel assay in Xenopus oocytes co-expressing NMDAR, PSD-95, and GKAP

    PMID:10570927

    Open questions at the time
    • Single in vitro reconstitution system
    • Mechanism of potentiation not defined
  4. 2003 High

    Established GKAP as an immobile static core of the PSD with N-terminus-dependent targeting independent of PSD-95.

    Evidence FRAP and deletion-construct localization in hippocampal neurons

    PMID:12581155

    Open questions at the time
    • Molecular identity of the N-terminal targeting receptor unknown
    • Did not address activity-dependent mobility
  5. 2005 High

    Revealed that the GKAP–SAP97 interaction is dynamically regulated, with p38γ/SAPK3 phosphorylation of SAP97 dissociating GKAP and releasing it from the cytoskeleton under osmotic stress.

    Evidence Kinase assay and phosphorylation-dependent co-IP dissociation in cells

    PMID:15729360

    Open questions at the time
    • Synaptic relevance of osmotic-stress regulation untested
    • GKAP itself not the phosphorylated target here
  6. 2010 High

    Identified TRIM3 as the E3 ligase that ubiquitinates GKAP for proteasomal degradation, coupling GKAP turnover to spine size and activity-induced PSD remodeling.

    Evidence Co-IP from PSD fractions, ubiquitination assay, RNAi with spine-morphology readout

    PMID:20352094

    Open questions at the time
    • Upstream signal triggering TRIM3 activity not defined in this study
    • Phosphodegron not yet mapped
  7. 2010 High

    Extended GKAP function beyond the synapse, showing it links Dlg1 to dynein to regulate microtubule dynamics and centrosome positioning in migrating cells downstream of Cdc42.

    Evidence Reciprocal co-IP, siRNA, and live imaging of centrosome positioning in migrating cells

    PMID:21041448

    Open questions at the time
    • Relationship between synaptic and migratory GKAP pools unknown
    • Whether dynein binding uses the same LC8 interface untested here
  8. 2011 High

    Demonstrated CDK5-dependent GKAP phosphorylation and degradation as the route by which amyloid-β collapses the synaptic actin cytoskeleton.

    Evidence In vitro CDK5 kinase assay, ubiquitination assay, and phospho-resistant mutant rescue in cortical neurons

    PMID:21829588

    Open questions at the time
    • CDK5 phosphosites not precisely mapped in this entry
    • Relationship to CaMKII/TRIM3 degradation pathway unresolved
  9. 2012 High

    Resolved the bidirectional phospho-code controlling GKAP abundance: CaMKIIα/Ser54 drives degradation while CaMKIIβ/Ser340-384 promotes accumulation by releasing GKAP from myosin Va, governing homeostatic scaling.

    Evidence Site-directed mutagenesis, isoform-specific CaMKII KD/OE, ubiquitination and synaptic scaling assays in hippocampal neurons

    PMID:23143515

    Open questions at the time
    • How the same residue selectively engages TRIM3 vs other ligases not fully detailed
    • In vivo scaling consequences not tested
  10. 2014 High

    Defined the quantitative architecture of GKAP–DLC2 oligomers in spines, showing DLC2 dimerization potentiates GKAP self-association into large complexes that drive spine-preferential localization and NMDA receptor activity.

    Evidence Two-photon sN&B fluorescence fluctuation microscopy, BRET, and NMDAR electrophysiology in living neurons

    PMID:22328512 PMID:24938595

    Open questions at the time
    • High-resolution structure of the oligomer not resolved here
    • How oligomer size is regulated by activity incompletely defined
  11. 2018 Medium

    Linked GKAP to disease contexts: required for normal PSD complex organization and sociability in mice, and a regulator of NMDAR-driven invasive growth in pancreatic neuroendocrine tumors.

    Evidence Knockout mouse with PSD biochemistry and behavior; GKAP knockdown plus NMDAR inhibition with invasion and expression profiling in tumor cells

    PMID:29396406 PMID:29606348

    Open questions at the time
    • Behavioral specificity and circuit basis not dissected
    • FMRP/HSF1 mechanistic link to GKAP in tumors correlative
  12. 2025 Medium

    Provided structural definition of GKAP's disordered interaction interfaces: a flexible Shank1-PDZ-binding C-terminus with flanking helical propensity, and a defined hexameric GKAP–LC8 complex of two GKAP and two LC8 dimers.

    Evidence NMR resonance assignment, affinity measurement, and MD simulations defining stoichiometry and dynamics

    PMID:40843979 PMID:41191219

    Open questions at the time
    • Structure of full-length GKAP within the PSD not determined
    • How disorder enables condensate formation untested in these studies

Open questions

Synthesis pass · forward-looking unresolved questions
  • It remains unresolved how GKAP's intrinsically disordered regions, oligomerization, and phase behavior integrate with its multiple competing phosphorylation/degradation inputs to set PSD scaffold size in vivo.
  • No integrated model linking condensate layering, oligomer state, and phospho-regulated turnover
  • In vivo, time-resolved scaffold dynamics during plasticity not established
  • Mechanistic distinction between synaptic and non-neuronal (centrosomal/adipocyte) functions unclear

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0060090 molecular adaptor activity 3 GO:0005198 structural molecule activity 2 GO:0008092 cytoskeletal protein binding 2 GO:0098772 molecular function regulator activity 2
Localization
GO:0005886 plasma membrane 3 GO:0005815 microtubule organizing center 2 GO:0005856 cytoskeleton 2
Pathway
R-HSA-112316 Neuronal System 3 R-HSA-392499 Metabolism of proteins 3 R-HSA-162582 Signal Transduction 2
Partners
CAMK2ACDK5DLG1DLG4DLGAP1DYNLL2SHANK1TRIM3
Complex memberships
Dlg1-GKAP-dynein complexGKAP-DLC2/DYNLL2 (LC8) hetero-oligomerShank/GKAP/PSD-95 postsynaptic density complex

Evidence

Reading pass · 22 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
1997 GKAP (DLGAP1) directly binds the guanylate kinase-like (GK) domain of all four known mammalian PSD-95 family members (PSD-95, SAP97, SAP98, SAP102); GKAP colocalizes and coimmunoprecipitates with PSD-95 in vivo and coclusters with PSD-95 and NMDA receptors/K+ channels in heterologous cells. Yeast two-hybrid, co-immunoprecipitation from rat brain, heterologous cell coexpression The Journal of cell biology High 9024696
1997 DAP-1 (DLGAP1) binds the guanylate kinase-like domains of hDLG and PSD-95, and associates with NMDA receptors, APC protein, colocalizing with PSD-95 and NMDA-R at hippocampal synapses. Yeast two-hybrid, co-immunoprecipitation, immunofluorescence in hippocampal neurons and mouse cerebellum Genes to cells : devoted to molecular & cellular mechanisms High 9286858
1999 Shank binds via its PDZ domain to the C-terminus of GKAP (DLGAP1), forming a ternary Shank/GKAP/PSD-95 complex that can be assembled in heterologous cells and coimmunoprecipitated from rat brain. A GKAP splice variant lacking the Shank-binding C-terminus inhibits synaptic localization of Shank. Yeast two-hybrid, co-immunoprecipitation from rat brain, heterologous cell reconstitution, neuronal expression of splice variants Neuron High 10433268
1999 GKAP markedly potentiates the channel activity of the NMDA receptor–PSD-95 complex without affecting basic channel properties or PSD-95-induced channel property changes alone, acting quantitatively via PSD-95. Electrophysiological channel activity assay in Xenopus oocytes expressing NMDA receptor, PSD-95, and GKAP FEBS letters Medium 10570927
1999 ProSAP1 and ProSAP2 (Shank family) PDZ domains interact with SAPAP/GKAP family proteins, verified by coimmunoprecipitation and cotransfection in HEK cells, providing a link between PSD-95-bound membrane receptors and the cytoskeleton. Yeast two-hybrid, co-immunoprecipitation, cotransfection in HEK cells Biochemical and biophysical research communications Medium 10527873
2000 SAP97 binding to GKAP is regulated by intramolecular interactions within SAP97: the SH3 domain and sequences between SH3 and GUK domains inhibit GKAP binding, while N-terminal sequences facilitate GKAP binding by associating with the SH3 domain. SAP97 can adopt a compact U-shaped conformation that occludes the GKAP-binding GUK domain. In vitro binding assays, domain deletion/mutation analysis, molecular modeling based on crystal structure data The EMBO journal High 11060025
2003 SAPAP1 (DLGAP1) synaptic targeting is mediated by its N-terminal region and is independent of interaction with PSD-95 or S-SCAM. SAPAP1 influences the synaptic localization of Shank but not PSD-95 or S-SCAM. FRAP analysis revealed SAPAP1 is immobile at synapses, comprising a static core of the PSD. Expression of SAPAP1 deletion constructs in hippocampal neurons, FRAP (fluorescence recovery after photobleaching), immunofluorescence Genes to cells : devoted to molecular & cellular mechanisms High 12581155
2005 SAPK3/p38γ MAP kinase phosphorylates SAP97/hDlg, triggering its dissociation from GKAP (DLGAP1) and release from the cytoskeleton in response to osmotic stress. Kinase assay, co-immunoprecipitation, phosphorylation-dependent dissociation assay, osmotic stress treatment of cells The EMBO journal High 15729360
2010 TRIM3 is an E3 ubiquitin ligase for GKAP/SAPAP1: TRIM3 stimulates ubiquitination and proteasome-dependent degradation of GKAP, reducing GKAP and Shank1 at postsynaptic sites and decreasing dendritic spine head size. RNAi of TRIM3 increases synaptic GKAP and Shank1 and enlarges spine heads, and prevents activity-induced GKAP loss. Co-immunoprecipitation from rat brain PSD fractions, ubiquitination assay, proteasome inhibitor treatment, RNAi knockdown in neurons, immunofluorescence of dendritic spines PloS one High 20352094
2010 Dlg1 interacts with dynein via GKAP during wound-induced cell migration; Dlg1, GKAP, and dynein together control microtubule dynamics near the cell cortex and promote centrosome positioning and cell polarity downstream of Cdc42. Co-immunoprecipitation, siRNA knockdown, immunofluorescence, live-cell imaging of centrosome positioning in migrating cells The Journal of cell biology High 21041448
2011 CDK5 phosphorylates GKAP (SAPAP1) directly, leading to its polyubiquitination and proteasomal degradation and collapse of the synaptic actin cytoskeleton in response to amyloid-β via NMDAR activity and Ca2+ influx. GKAP mutants resistant to CDK5 phosphorylation prevent actin cytoskeleton collapse. In vitro kinase assay (CDK5 on GKAP), ubiquitination assay, pharmacological inhibition of NMDAR/CDK5, expression of phosphorylation-resistant GKAP mutants in rat cortical neurons PloS one High 21829588
2012 GKAP-DLC2 (DYNLL2) interaction stabilizes scaffolding protein expression at the PSD and enhances synaptic NMDA receptor activity. The interaction is favored by sustained synaptic activity. BRET imaging in living neurons, immunostaining, electrophysiological recording of NMDA receptor currents Journal of cell science High 22328512
2012 CaMKIIα activated by NMDA receptors phosphorylates GKAP Ser54 to induce polyubiquitination and proteasomal degradation of GKAP (overexcitation-dependent). CaMKIIβ activated via L-type VDCCs phosphorylates GKAP Ser340 and Ser384 to promote GKAP recruitment by uncoupling it from the myosin Va motor complex. Bidirectional changes in synaptic GKAP govern homeostatic synaptic scaling and PSD remodeling. Site-directed mutagenesis of GKAP phosphorylation sites, CaMKII isoform-specific knockdown/overexpression, ubiquitination assay, immunofluorescence in rat hippocampal neurons, synaptic scaling assay Nature neuroscience High 23143515
2014 In dendritic spines, DLC2 (DYNLL2) dimerization is required for interaction with GKAP, which in turn potentiates GKAP self-association. Spines contain large GKAP-DLC2 hetero-oligomers (~16 DLC2 and ~13 GKAP monomers), whereas dendritic shafts contain smaller complexes (~2 DLC2, ~2 GKAP). Disruption of the GKAP-DLC2 interaction destabilizes oligomers, reduces spine-preferential GKAP localization, and inhibits NMDA receptor activity. Two-photon scanning number and brightness (sN&B) fluorescence fluctuation microscopy in living neurons, BRET, NMDA receptor electrophysiology Journal of cell science High 24938595
2018 GKAP governs invasive growth and NMDAR inhibitor treatment response in pancreatic neuroendocrine tumors via its role in regulating NMDAR pathway activity. Downstream effectors include FMRP and HSF1, which support invasiveness along with GKAP. Genetic knockdown of GKAP combined with pharmacological NMDAR inhibition, genome-wide expression profiling, in vitro invasion assays Cancer cell Medium 29606348
2018 Dlgap1 knockout mice show disruption of PSD protein interactions (biochemically assessed) and selective deficits in sociability, establishing DLGAP1 as necessary for normal PSD protein complex organization and social behavior. Knockout mouse model, co-immunoprecipitation/biochemical fractionation of PSD complexes, behavioral battery Scientific reports Medium 29396406
2019 DLGAP1 localizes to centrosomes in hematopoietic cells in a cell-cycle-dependent manner; its dissociation from centrosomes is promoted by Jak2, SRC, MAPK, and CDK1 kinases. DLGAP1 negatively affects growth rate of MPL-dependent hematopoietic cells and supports megakaryocytic polyploidization, correlated with centrosomal dissociation. Retroviral insertional mutagenesis screen, immunofluorescence for centrosomal localization across cell cycle, overexpression/shRNA knockdown in hematopoietic cell lines, flow cytometry for polyploidization Biomarker research Medium 31321035
2024 Acute elimination of GKAP (but not PSD-95) reduces postsynaptic scaffold size at individual synapses, demonstrating a direct structural role of GKAP in maintaining PSD scaffold assembly. Auxin-inducible degron 2 (AID2) technology for acute protein elimination in cultured neurons and in vivo, live imaging of scaffold size bioRxivpreprint Medium bio_10.1101_2024.10.20.619267
2025 The GKAP C-terminal region (Ct43) is intrinsically disordered but binds the Shank1 PDZ domain with micromolar affinity; NMR chemical shifts reveal two regions of helical propensity flanking the PDZ-binding motif. NMR resonance assignment (1H, 13C, 15N), affinity measurement of Shank1 PDZ binding Biomolecular NMR assignments Medium 41191219
2025 GKAP forms a well-defined hexameric complex with LC8 (DYNLL): two GKAP molecules and two LC8 dimers. The LC8-binding segment of GKAP is intrinsically disordered and retains substantial flexibility even within the complex; the linker region between the two LC8-binding sites remains flexible. NMR spectroscopy, molecular dynamics calculations, stoichiometry determination The FEBS journal High 40843979
2024 nArgBP2, GKAP, and SHANK3 form biomolecular condensates with a layered organization: nArgBP2 in the inner phase, SHANK3 in the outer phase, and GKAP partially in both. CaMKIIα activation disperses most condensates and redistributes GKAP and SHANK3 within remaining condensates. Live-cell fluorescence imaging of condensates in fibroblasts, coexpression of tagged proteins, CaMKIIα activation experiment Frontiers in cellular neuroscience Medium 38440150
2020 Dlgap1 overexpression in brown adipocytes inhibits brown-fat-related gene expression and promotes white-fat-related gene expression, while increasing proliferation and apoptosis. Knockout of Dlgap1 in white fat cells promotes brown-fat gene expression, inhibits white-fat gene expression, and inhibits proliferation and apoptosis while promoting maturation, establishing DLGAP1 as a negative regulator of white adipocyte browning. Overexpression and knockout in cultured white and brown adipocytes, gene expression analysis, proliferation and apoptosis assays Lipids in health and disease Low 32169116

Source papers

Stage 0 corpus · 67 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
1999 Shank, a novel family of postsynaptic density proteins that binds to the NMDA receptor/PSD-95/GKAP complex and cortactin. Neuron 841 10433268
1997 GKAP, a novel synaptic protein that interacts with the guanylate kinase-like domain of the PSD-95/SAP90 family of channel clustering molecules. The Journal of cell biology 445 9024696
2005 p38gamma regulates the localisation of SAP97 in the cytoskeleton by modulating its interaction with GKAP. The EMBO journal 206 15729360
1999 Proline-rich synapse-associated proteins ProSAP1 and ProSAP2 interact with synaptic proteins of the SAPAP/GKAP family. Biochemical and biophysical research communications 154 10527873
2012 GKAP orchestrates activity-dependent postsynaptic protein remodeling and homeostatic scaling. Nature neuroscience 114 23143515
1997 DAP-1, a novel protein that interacts with the guanylate kinase-like domains of hDLG and PSD-95. Genes to cells : devoted to molecular & cellular mechanisms 110 9286858
2000 Intramolecular interactions regulate SAP97 binding to GKAP. The EMBO journal 96 11060025
2010 Degradation of postsynaptic scaffold GKAP and regulation of dendritic spine morphology by the TRIM3 ubiquitin ligase in rat hippocampal neurons. PloS one 90 20352094
2020 Long non-coding RNA DLGAP1-AS1 facilitates tumorigenesis and epithelial-mesenchymal transition in hepatocellular carcinoma via the feedback loop of miR-26a/b-5p/IL-6/JAK2/STAT3 and Wnt/β-catenin pathway. Cell death & disease 84 31949128
2018 GKAP Acts as a Genetic Modulator of NMDAR Signaling to Govern Invasive Tumor Growth. Cancer cell 75 29606348
2010 Dlg1 binds GKAP to control dynein association with microtubules, centrosome positioning, and cell polarity. The Journal of cell biology 69 21041448
2022 Long noncoding RNA DLGAP1-AS2 promotes tumorigenesis and metastasis by regulating the Trim21/ELOA/LHPP axis in colorectal cancer. Molecular cancer 46 36376892
2018 Dlgap1 knockout mice exhibit alterations of the postsynaptic density and selective reductions in sociability. Scientific reports 40 29396406
2021 N6-methyladenosine (m6A)-mediated lncRNA DLGAP1-AS1enhances breast canceradriamycin resistance through miR-299-3p/WTAP feedback loop. Bioengineered 39 34866525
2020 LncRNA DLGAP1-AS2 modulates glioma development by up-regulating YAP1 expression. Journal of biochemistry 31 31899508
2011 CDK5 is essential for soluble amyloid β-induced degradation of GKAP and remodeling of the synaptic actin cytoskeleton. PloS one 31 21829588
2019 Long noncoding RNA DLGAP1-AS1 promotes cell proliferation in hepatocellular carcinoma via sequestering miR-486-5p. Journal of cellular biochemistry 29 31633236
1999 The ubiquitin-homology protein, DAP-1, associates with tumor necrosis factor receptor (p60) death domain and induces apoptosis. The Journal of biological chemistry 29 10187798
2022 METTL3-induced DLGAP1-AS2 promotes non-small cell lung cancer tumorigenesis through m6A/c-Myc-dependent aerobic glycolysis. Cell cycle (Georgetown, Tex.) 27 35972892
2012 Enhancing the promiscuous phosphotriesterase activity of a thermostable lactonase (GkaP) for the efficient degradation of organophosphate pesticides. Applied and environmental microbiology 27 22798358
2022 DLGAP1-AS2-Mediated Phosphatidic Acid Synthesis Activates YAP Signaling and Confers Chemoresistance in Squamous Cell Carcinoma. Cancer research 26 35731019
1997 Differential expression of isoforms of PSD-95 binding protein (GKAP/SAPAP1) during rat brain development. FEBS letters 26 9428732
2019 The role of microRNA-148a and downstream DLGAP1 on the molecular regulation and tumor progression on human glioblastoma. Oncogene 24 31477833
2021 The lncRNA DLGAP1-AS1/miR-149-5p/TGFB2 axis contributes to colorectal cancer progression and 5-FU resistance by regulating smad2 pathway. Molecular therapy oncolytics 23 33816780
2018 HOTAIRM1 competed endogenously with miR-148a to regulate DLGAP1 in head and neck tumor cells. Cancer medicine 23 29905017
2020 Learning and reaction times in mouse touchscreen tests are differentially impacted by mutations in genes encoding postsynaptic interacting proteins SYNGAP1, NLGN3, DLGAP1, DLGAP2 and SHANK2. Genes, brain, and behavior 22 33347690
2021 Long noncoding RNA DLGAP1-AS2 facilitates Wnt1 transcription through physically interacting with Six3 and drives the malignancy of gastric cancer. Cell death discovery 19 34545072
2020 The long noncoding RNA DLGAP1-AS2 facilitates cholangiocarcinoma progression via miR-505 and GALNT10. FEBS open bio 19 33301605
2012 GKAP-DLC2 interaction organizes the postsynaptic scaffold complex to enhance synaptic NMDA receptor activity. Journal of cell science 19 22328512
2012 Genetic analysis of the DLGAP1 gene as a candidate gene for schizophrenia. Psychiatry research 19 22940546
2003 Synaptic localization of SAPAP1, a synaptic membrane-associated protein. Genes to cells : devoted to molecular & cellular mechanisms 19 12581155
2021 A genome-wide association study identifies a novel candidate locus at the DLGAP1 gene with susceptibility to resistant hypertension in the Japanese population. Scientific reports 18 34593835
2023 The knockdown of lncRNA DLGAP1-AS2 suppresses osteosarcoma progression by inhibiting aerobic glycolysis via the miR-451a/HK2 axis. Cancer science 17 37817462
2022 LncRNA DLGAP1-AS2 promotes the radioresistance of rectal cancer stem cells by upregulating CD151 expression via E2F1. Translational oncology 17 35144091
2020 lncRNA DLGAP1-AS2 Knockdown Inhibits Hepatocellular Carcinoma Cell Migration and Invasion by Regulating miR-154-5p Methylation. BioMed research international 17 33195697
2021 LncRNA DLGAP1-AS1 accelerates glioblastoma cell proliferation through targeting miR-515-5p/ROCK1/NFE2L1 axis and activating Wnt signaling pathway. Brain and behavior 16 34536977
2017 Death associated protein 1 (DAP 1) positively regulates virus replication and apoptosis of hemocytes in shrimp Marsupenaeus japonicus. Fish & shellfish immunology 16 28212834
1999 Activation of channel activity of the NMDA receptor-PSD-95 complex by guanylate kinase-associated protein (GKAP). FEBS letters 16 10570927
2020 Long Noncoding RNA DLGAP1-AS1 Promotes the Aggressive Behavior of Gastric Cancer by Acting as a ceRNA for microRNA-628-5p and Raising Astrocyte Elevated Gene 1 Expression. Cancer management and research 15 32431541
2021 Long noncoding RNA DLGAP1-AS1 promotes the progression of glioma by regulating the miR-1297/EZH2 axis. Aging 14 33901010
2021 LncRNA DLGAP1-AS2 regulates miR-503/cyclin D1 to promote cell proliferation in non-small cell lung cancer. BMC pulmonary medicine 14 34454450
2015 Huntingtin-associated protein 1 (HAP1) is a cGMP-dependent kinase anchoring protein (GKAP) specific for the cGMP-dependent protein kinase Iβ isoform. The Journal of biological chemistry 14 25653285
2022 DLGAP1-AS2 promotes estrogen receptor signalling and confers tamoxifen resistance in breast cancer. Molecular biology reports 13 35449318
2021 Long non-coding RNA DLGAP1-AS1 promotes the progression of gastric cancer via miR-515-5p/MARK4 axis. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 12 34037089
2019 Downregulation of DLGAP1-Antisense RNA 1 Alleviates Vascular Endothelial Cell Injury Via Activation of the Phosphoinositide 3-kinase/Akt Pathway Results from an Acute Limb Ischemia Rat Model. European journal of vascular and endovascular surgery : the official journal of the European Society for Vascular Surgery 12 31744785
2022 LncRNA DLGAP1-AS2 overexpression associates with gastric tumorigenesis: a promising diagnostic and therapeutic target. Molecular biology reports 11 34981339
2018 DLGAP1 and NMDA receptor-associated postsynaptic density protein genes influence executive function in attention deficit hyperactivity disorder. Brain and behavior 11 29484270
2003 Mutation and association analysis of the DAP-1 gene with schizophrenia. Psychiatry and clinical neurosciences 11 12950712
2014 The stoichiometry of scaffold complexes in living neurons - DLC2 functions as a dimerization engine for GKAP. Journal of cell science 9 24938595
2020 Dlgap1 negatively regulates browning of white fat cells through effects on cell proliferation and apoptosis. Lipids in health and disease 8 32169116
2022 Long non-coding RNA DLGAP1-AS1 modulates the development of non-small-cell lung cancer via the microRNA-193a-5p/DTL axis. Laboratory investigation; a journal of technical methods and pathology 7 36183046
2024 Investigating the effect of LncRNA DLGAP1-AS2 suppression on chemosensitivity of gastric cancer to chemotherapy. Naunyn-Schmiedeberg's archives of pharmacology 6 38748228
2022 DLGAP1-AS2 promotes human colorectal cancer progression through trans-activation of Myc. Mammalian genome : official journal of the International Mammalian Genome Society 6 36222892
2023 Long Non-coding RNA DLGAP1-AS1 and DLGAP1-AS2: Two Novel Oncogenes in Multiple Cancers. Current medicinal chemistry 5 36121088
2025 Residual flexibility in the topologically constrained multivalent complex between the GKAP scaffold and LC8 hub proteins. The FEBS journal 4 40843979
2015 Genome-Wide Association Study of HIV-Related Lipoatrophy in Thai Patients: Association of a DLGAP1 Polymorphism with Fat Loss. AIDS research and human retroviruses 4 25950743
2024 Late development of OCD-like phenotypes in Dlgap1 knockout mice. Psychopharmacology 3 39177810
2022 Long non-coding RNA DLGAP1 antisense RNA 1 accelerates glioma progression via the microRNA-628-5p/DEAD-box helicase 59 pathway. Clinics (Sao Paulo, Brazil) 3 35113786
2019 DLGAP1 directs megakaryocytic growth and differentiation in an MPL dependent manner in hematopoietic cells. Biomarker research 3 31321035
2025 Improvement of carboplatin chemosensitivity in lung cancer cells by siRNA-mediated downregulation of DLGAP1-AS2 expression. Scientific reports 2 40055367
2024 Association of rs11081062 polymorphism of DLGAP1 gene and levels of SLC1A1 protein with obsessive-compulsive disorder. Nucleosides, nucleotides & nucleic acids 2 38593060
2024 nArgBP2 together with GKAP and SHANK3 forms a dynamic layered structure. Frontiers in cellular neuroscience 1 38440150
2026 Rare protein-disrupting variants in NPY5R, DLGAP1 and MAPK8IP3 segregate with OCD in two multiplex pedigrees. medRxiv : the preprint server for health sciences 0 42078338
2025 1H, 13C, and 15N resonance assignment of the C terminal region of the disordered postsynaptic scaffold protein GKAP. Biomolecular NMR assignments 0 41191219
2024 Deciphering the Role of miR-30a-5p and DLGAP1 Gene in Non-small Cell Lung Cancer. Anticancer research 0 38821626
2021 Long Noncoding RNA DLGAP1-AS1 Promotes the Aggressive Behavior of Gastric Cancer by Acting as a ceRNA for microRNA-628-5p and Raising Astrocyte Elevated Gene 1 Expression [Retraction]. Cancer management and research 0 34611445
2015 [The Hydrothermal Synthesis, Structure and Spectroscopy Study on (H2dap)6H[V12B16O54(OH)4] · 12H2O (dap = 1, 2-diaminopropane)]. Guang pu xue yu guang pu fen xi = Guang pu 0 26669179

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