| 1998 |
FIBP (aFGF intracellular binding protein) was identified as a novel 42 kDa intracellular protein that binds selectively to mitogenic acidic FGF (aFGF) but not to the non-mitogenic mutant aFGF-K132E, indicating specificity for the mitogenic conformation. In vitro-translated FIBP bound specifically to a maltose-binding protein–aFGF fusion protein. The protein localizes predominantly to the nucleus and, to a lesser extent, to mitochondria and other cytoplasmic membranes, including post-translational association with microsomes. |
Yeast two-hybrid screen, in vitro binding assay (MBP-aFGF pulldown), cell-free translation + microsome association, immunoblot, fluorescence microscopy |
The Biochemical journal |
High |
9806903
|
| 2000 |
The human FIBP gene spans >5 kb, comprises ten exons and nine introns, and maps to chromosome 11q13.1. Two splice variants exist in different tissues. Functional promoter analysis with a luciferase reporter system localized strong transcriptional activity to within 600 bp of the 5′ flanking region. Comparative analysis showed FIBP is evolutionarily conserved across human, mouse, and Drosophila. |
Genomic mapping, splice variant cloning and sequencing, luciferase reporter assay |
The Biochemical journal |
Medium |
11104667
|
| 2003 |
A Drosophila FIBP homologue (DrFIBP) was cloned and characterized; it shares conserved structural architecture with human FIBP. DrFIBP is expressed during embryonic, larval, and adult stages and is spatially expressed in the developing tracheal system and ventral midline cells — two known sites of FGF signaling — as determined by whole-mount embryo immunostaining. |
Cloning, comparative sequence analysis, RT-PCR, whole-mount embryo immunostaining |
Gene |
Medium |
12801646
|
| 2008 |
RNA interference-mediated inhibition of FIBP in bovine granulosa cells in vitro inhibited estradiol production, suggesting FIBP promotes estradiol biosynthesis in granulosa cells of regressing subordinate follicles. |
RNA interference (RNAi) knockdown in primary granulosa cells, estradiol production assay |
Physiological genomics |
Medium |
18285519
|
| 2014 |
FIBP was identified as a novel CDK5-binding partner in non-neuronal cells. FIBP, CDK5, and KIAA0528 form a stable trimeric complex, with KIAA0528 and FIBP both required for the assembly and stability of the complex. Depletion of FIBP (or CDK5 or KIAA0528) in breast cancer cells impaired cell proliferation and decreased migration. |
Proteomic CDK family interactome (AP-MS with SAINT analysis), Co-IP validation, siRNA knockdown with proliferation and migration assays |
Molecular & cellular proteomics : MCP |
Medium |
25096995
|
| 2016 |
A homozygous nonsense FIBP variant in a patient caused FIBP mRNA degradation (likely NMD) and resulted in increased proliferation capacity of the patient's fibroblasts compared with controls, linking FIBP loss-of-function to enhanced cell proliferation in the context of an overgrowth syndrome. |
Exome sequencing, RT-PCR (cDNA degradation), in vitro cellular proliferation assay |
Clinical genetics |
Medium |
26660953
|
| 2016 |
An in-frame insertion in FIBP (p.H59LN) in three affected siblings with an overgrowth syndrome caused increased proliferation capacity in patient skin fibroblasts compared to controls. In situ hybridization in mouse embryos detected Fibp expression most notably in the brain, suggesting a role in cognitive development. |
Whole-genome genotyping, whole-exome sequencing, in vitro cellular proliferation assay, in situ hybridization in mouse embryos |
American journal of medical genetics. Part A |
Medium |
27183861
|
| 2018 |
FIBP knockdown in colorectal cancer stem cells inhibited proliferation, enhanced chemotherapy sensitivity, and reduced stemness markers in vitro and in vivo. Mechanistically, FIBP bound to GSK3β and inhibited its phosphorylation at Tyr216, thereby activating β-catenin/TCF/cyclin D1 signaling. Additional GSK3β knockdown reversed FIBP-silencing-induced inhibition of proliferation. FIBP also regulated stemness via methylation activity dependent on GSK3β but independent of β-catenin. |
shRNA knockdown, Co-IP (FIBP–GSK3β interaction), phosphorylation assay, RNA-seq + GSEA, in vivo xenograft, GSK3β rescue knockdown, DNA methylation profiling |
Oncogenesis |
High |
30275459
|
| 2022 |
FIBP knockdown in murine and human CD8+ T cells significantly enhanced T cell-mediated cancer killing in in vitro co-culture assays. Fibp knockout in murine T cells potentiated the in vivo efficacy of adoptive cell transfer in the B16 melanoma model. Mechanistically, Fibp knockout T cells exhibited reduced cholesterol metabolism, which was identified as the pathway through which FIBP inhibits effector T cell function. |
CRISPR/Cas9 knockout in murine and human CD8+ T cells, in vitro tumor co-culture killing assay, in vivo adoptive cell transfer (B16 tumor model), cholesterol metabolism profiling |
Nature medicine |
High |
35501486
|
| 2023 |
FIBP interacts with transcription factor STAT3 to enhance its transcriptional activity, leading to induced expression of the downstream target gene EME1. Depletion of FIBP inhibited lung adenocarcinoma progression and radioresistance in vitro and in vivo, and the biological effects of FIBP were partially dependent on EME1. |
Co-IP (FIBP–STAT3 interaction), STAT3 transcriptional activity assay, EME1 expression analysis, FIBP knockdown in vitro and in vivo, EME1 rescue experiments |
International journal of biological sciences |
Medium |
37564211
|
| 2025 |
USP5 was found to interact with a protein complex containing FIBP, C2CD5, and CDK5, as identified by co-immunoprecipitation coupled with mass spectrometry in AML cells, confirming FIBP participates in this multi-protein complex. |
Co-immunoprecipitation coupled with mass spectrometry |
Biochemical pharmacology |
Low |
41344512
|