Affinage

FBXO3

F-box only protein 3 · UniProt Q9UK99

Length
471 aa
Mass
54.6 kDa
Annotated
2026-06-09
61 papers in source corpus 17 papers cited in narrative 17 extracted findings
Cross-family judge vs UniProt: Affinage preferred faithfulness: 8/8 claims corpus-supported (100%)

Mechanistic narrative

Synthesis pass · prose summary of the discoveries below

FBXO3 is the substrate-recognition subunit of an SCF (SKP1–CUL1–RBX1) E3 ubiquitin ligase that controls inflammatory, neuronal, and oncogenic signaling by directing target proteins for polyubiquitination and proteasomal degradation (PMID:24123678, PMID:27365398, PMID:39921442). Its C-terminal ApaG domain adopts an immunoglobulin/fibronectin-III fold whose loop 1 mediates substrate engagement; this domain is necessary but not sufficient for binding, and it is essential for productive substrate disposal (PMID:27010866, PMID:24123678). A central recurring axis is FBXO3-driven degradation of the competing F-box protein FBXL2, which de-represses TRAF proteins to amplify proinflammatory cytokine production (PMID:24123678) and, in spinal dorsal horn neurons, stabilizes the FBXL2 substrates TRAF2 and RIM1α to drive GluR1 and CaV2.2-dependent synaptic potentiation underlying neuropathic allodynia (PMID:26674878, PMID:27629721). FBXO3 acts on a broad substrate range with diverse outcomes: it degrades Smurf1 and other Nedd4-family ligases (PMID:25721664), the disease-relevant C21ORF2 in a manner reversed by NEK1 phosphorylation (PMID:32891887), CARM1 to epigenetically silence K+ channel genes (PMID:33415686), HIPK2 during cerebral ischemia (PMID:36362432), and DUSP9 to sustain MAPK signaling and leukemia stem cell maintenance in CML (PMID:41850237). It also ubiquitylates AIRE to potentiate P-TEFb-dependent transcription (PMID:27365398) and operates in a TBK1-phosphorylation-dependent lysophagy axis through TMEM192 (PMID:40083080). Not all FBXO3 functions require its catalytic ligase activity: it stabilizes USP4 by displacing the aminopeptidase DNPEP to promote breast cancer metastasis (PMID:38134227). FBXO3 activity is itself regulated by signaling and RNA-modification inputs, including ERK1-dependent phosphorylation and m6A/YTHDF1-controlled protein stability (PMID:38134227, PMID:38622374). Pharmacological inhibition of FBXO3 (BC-1215) blunts cytokine production, neuropathic pain, and CML stem cell maintenance, establishing it as a tractable drug target (PMID:24123678, PMID:26674878, PMID:41850237).

Mechanistic history

Synthesis pass · year-by-year structured walk · 11 steps
  1. 2013 High

    Established FBXO3 as an SCF F-box protein with a defined immunological output by showing it degrades the competing F-box protein FBXL2 to release TRAF-driven cytokine production.

    Evidence Co-IP, siRNA knockdown, cytokine assays, and murine inflammation models with an ApaG-targeting inhibitor

    PMID:24123678

    Open questions at the time
    • Linkage type and direct ubiquitin-transfer reconstitution not defined
    • ApaG residues mediating FBXL2 recognition not yet mapped
  2. 2014 High

    Showed FBXO3 can be hijacked by a viral virulence factor to degrade a TFIIH subunit, revealing it as a host substrate receptor for transcriptional suppression and SCF assembly via Skp1.

    Evidence siRNA depletion with p62 rescue and IFN reporter assays plus Co-IP in RVFV-infected cells

    PMID:24403578

    Open questions at the time
    • Cullin requirement was paradoxical (cullin1/7 and Rbx1 knockdown did not rescue p62)
    • Direct ubiquitination of p62 not reconstituted
  3. 2015 High

    Extended the FBXO3–FBXL2 axis into neuropathic pain and broadened the substrate range to Nedd4-family HECT ligases, indicating FBXO3 regulates other ubiquitin enzymes.

    Evidence Spinal nerve ligation rat model with BC-1215 inhibition (TRAF2/GluR1 axis) and Co-IP/ubiquitination assays for Smurf1/Nedd4 degradation

    PMID:25721664 PMID:26674878

    Open questions at the time
    • Smurf1/Nedd4 substrate selectivity over Fbxl15 not structurally explained
    • In vivo relevance of Nedd4-family degradation to BMP signaling untested
  4. 2016 High

    Defined the structural basis of FBXO3 substrate engagement and showed it can act as a non-degradative activator of transcription, indicating ubiquitylation outcomes are context-dependent.

    Evidence X-ray crystallography of the ApaG domain with loop mutagenesis/NMR/Co-IP, plus AIRE ubiquitylation, phospho-site mutagenesis, and transcription reporters

    PMID:27010866 PMID:27365398 PMID:27629721

    Open questions at the time
    • Why isolated ApaG is insufficient for substrate binding not resolved
    • Mechanism by which AIRE ubiquitylation enhances P-TEFb binding not detailed
  5. 2020 High

    Demonstrated phosphorylation-gated substrate choice and linkage-specific ubiquitination, connecting FBXO3 to disease-relevant proteostasis (C21ORF2/NEK1) and conserved antiviral IRF control.

    Evidence Co-IP, ubiquitylation assays, NEK1 phospho-epistasis and ALS mutant analysis; zebrafish fbxo3 knockout with K27-linkage-specific irf3/irf7 ubiquitination

    PMID:32859728 PMID:32891887

    Open questions at the time
    • F-box-independent IRF degradation mechanism in zebrafish unexplained
    • Conservation of K27-linkage activity to human FBXO3 not tested
  6. 2021 High

    Connected FBXO3 to epigenetic gene regulation by showing it degrades the methyltransferase CARM1 to silence K+ channel genes in neuropathic pain.

    Evidence Spinal nerve ligation model, ChIP for H3R17me2 at K+ channel promoters, and BC-1215 inhibition

    PMID:33415686

    Open questions at the time
    • Direct FBXO3–CARM1 interaction interface not mapped
    • Substrate-recognition determinants for CARM1 unknown
  7. 2022 Medium

    Implicated FBXO3 in ischemic neuroinflammation through HIPK2 turnover, expanding its substrate set.

    Evidence MCAO/R and OGD/R models with siRNA and BC-1215, monitoring HIPK2 levels and cytokines

    PMID:36362432

    Open questions at the time
    • FBXO3–HIPK2 interaction inferred rather than shown by Co-IP
    • Single lab without reciprocal validation
  8. 2023 High

    Revealed a catalysis-independent function in which FBXO3 stabilizes USP4 by displacing DNPEP, showing FBXO3 can act as a scaffold to promote, not destroy, partner stability and drive metastasis.

    Evidence Reciprocal Co-IP of FBXO3–USP4 and USP4–DNPEP, knockdown, mouse metastasis models, and ERK1 phosphorylation analysis

    PMID:38134227

    Open questions at the time
    • Structural basis for DNPEP displacement unknown
    • How E3-independent and E3-dependent roles are partitioned in cells unclear
  9. 2024 Medium

    Identified upstream control of FBXO3 abundance via m6A/YTHDF1 and a new mitochondrial substrate (PGC-1α), linking FBXO3 to metabolic regulation in acute lung injury.

    Evidence MeRIP/RIP assays, Co-IP of FBXO3–YTHDF1 and FBXO3–PGC-1α, ubiquitination assays, and CLP/LPS sepsis models

    PMID:38622374

    Open questions at the time
    • Direct ubiquitin transfer to PGC-1α not reconstituted
    • Single-lab abstract-level mechanistic depth
  10. 2025 Medium

    Resolved the full SCF(FBXO3) architecture and defined a phosphorylation-gated lysophagy role, clarifying both how FBXO3 assembles into the ligase and how it is signal-activated.

    Evidence Cryo-EM of CUL1-RBX1-SKP1-FBXO3 at 3.70 Å; TBK1-dependent phospho-FBXO3 promoting TMEM192 ubiquitination and TAX1BP1-mediated lysophagy

    PMID:39921442 PMID:40083080

    Open questions at the time
    • Structure lacks mutagenesis/activity validation and neddylation-state confirmation
    • How TBK1 phosphorylation alters FBXO3 substrate selectivity not defined
  11. 2026 High

    Established a selective oncogenic dependency by showing FBXO3 degrades DUSP9 to sustain MAPK signaling and CML leukemia stem cell maintenance with minimal effect on normal HSCs.

    Evidence Co-IP, ubiquitination assays, in vitro/in vivo CML LSC knockdown with DUSP9 rescue epistasis and pharmacological inhibition

    PMID:41850237

    Open questions at the time
    • DUSP9-binding determinants on FBXO3 unmapped
    • Basis for selectivity toward LSCs over normal HSCs unexplained

Open questions

Synthesis pass · forward-looking unresolved questions
  • How FBXO3 achieves its strikingly broad and partly non-catalytic substrate repertoire, and how upstream phosphorylation and m6A inputs are integrated to select among them, remains unresolved.
  • No common substrate-recognition motif identified across the diverse targets
  • Linkage specificity and neddylation dependence not systematically compared across substrates
  • Catalytic versus scaffolding functions not delineated genome-wide

Mechanism profile

Synthesis pass · controlled-vocabulary classification · explore literature graph →
Molecular activity
GO:0140096 catalytic activity, acting on a protein 5 GO:0016874 ligase activity 2 GO:0098772 molecular function regulator activity 2
Pathway
R-HSA-392499 Metabolism of proteins 4 R-HSA-162582 Signal Transduction 3 R-HSA-168256 Immune System 3
Partners
AIREC21ORF2CUL1DUSP9FBXL2SKP1TMEM192USP4
Complex memberships
SCF(SKP1-CUL1-RBX1-FBXO3) E3 ubiquitin ligase

Evidence

Reading pass · 17 per-paper findings extracted from the source corpus
Year Finding Method Journal Conf PMIDs
2013 FBXO3 functions as an E3 ubiquitin ligase F-box component that mediates the polyubiquitination and proteasomal degradation of FBXL2, thereby preventing FBXL2 from inhibiting TRAF proteins. This leads to elevated TRAF protein levels and potent stimulation of proinflammatory cytokine secretion from human inflammatory cells. The C-terminal ApaG domain of FBXO3 was found to be indispensable for mediating FBXL2 disposal and cytokine secretion. Co-immunoprecipitation, siRNA knockdown, cytokine secretion assays, murine models of viral pneumonia/septic shock/colitis, small-molecule inhibitor targeting ApaG domain Journal of immunology High 24123678
2014 RVFV NSs virulence factor recruits host cell F-box protein FBXO3 to degrade TFIIH subunit p62 via the ubiquitin/proteasome pathway, thereby suppressing type I interferon transcription. siRNA depletion of FBXO3 rescued p62 levels and elevated IFN transcription ~10-fold. FBXO3 forms SCF complexes with Skp1; knockdown of Skp1 also protected p62, but knockdown of cullin 1, cullin 7, or Rbx1 did not rescue p62 degradation. siRNA knockdown, co-immunoprecipitation, protein level rescue assays, IFN transcription reporter assays Journal of virology High 24403578
2015 FBXO3-dependent ubiquitination and degradation of FBXL2 leads to de-ubiquitination (stabilization) of TRAF2 in spinal dorsal horn neurons. This TRAF2 stabilization drives TNIK/GluR1 phosphorylation and GluR1 trafficking to the plasma membrane, mediating neuropathic allodynia after spinal nerve ligation. Intrathecal BC-1215 (FBXO3 inhibitor) prevented FBXL2 ubiquitination and ameliorated allodynia. Spinal nerve ligation rat model, siRNA knockdown, intrathecal pharmacological inhibition (BC-1215), behavioral allodynia assays, Western blot for ubiquitination and phosphorylation The Journal of neuroscience High 26674878
2015 FBXO3 targets Smurf1 (an HECT-type E3 ubiquitin ligase) for polyubiquitination and proteasomal degradation. Unlike Fbxl15, FBXO3 also targets all Nedd4 family members for degradation, implicating FBXO3 in BMP signaling through control of Nedd4-family ligase stability. Co-immunoprecipitation, ubiquitination assays, proteasomal degradation assays Biochemical and biophysical research communications Medium 25721664
2016 FBXO3 promotes ubiquitylation and increases transcriptional activity of AIRE (Autoimmune Regulator). AIRE, phosphorylated on two specific N-terminal residues, binds the SCF(FBXO3) (SKP1-CUL1-FBXO3) complex, which ubiquitylates AIRE and enhances its binding to P-TEFb (positive transcription elongation factor b), thereby potentiating AIRE-driven transcription of tissue-specific antigens in the thymus. Co-immunoprecipitation, ubiquitylation assays, transcriptional reporter assays, phosphorylation mapping, mutagenesis of phosphorylation sites The Journal of biological chemistry High 27365398
2016 The X-ray crystal structure of the human FBXO3 ApaG domain (residues 278–407) was solved at 2.0 Å resolution, revealing a classic Immunoglobulin/Fibronectin III-type fold with a seven-stranded β-sheet core and four extended loops. Loop 1 (residues 294–303) is critical for interaction with FBXL2 in the context of the full-length protein. The isolated ApaG domain alone is necessary but not sufficient for binding full-length FBXL2, as shown by coimmunoprecipitation. No binding to Mg²⁺, Co²⁺, or dinucleotide polyphosphates was detected. X-ray crystallography (2.0 Å), NMR titration, coimmunoprecipitation, loop mutagenesis The FEBS journal High 27010866
2016 Spinal nerve ligation induces Fbxo3-dependent Fbxl2 ubiquitination, reducing Fbxl2-mediated ubiquitination of the presynaptic active zone protein RIM1α. De-ubiquitinated (stabilized) RIM1α accumulates in synaptic plasma membranes and directly binds CaV2.2, increasing CaV2.2 expression and synaptic vesicle exocytosis in dorsal horn, thus driving neuropathic allodynia. Spinal nerve ligation rat model, siRNA knockdown, intrathecal BC-1215 administration, co-immunoprecipitation (RIM1α–CaV2.2 interaction), electrophysiology (sEPSC), Western blot for synaptic plasma membrane fractions The Journal of neuroscience High 27629721
2020 Zebrafish fbxo3 interacts with transcription factors irf3 and irf7 and specifically catalyzes K27-linked polyubiquitination of irf3 and irf7, targeting them for proteasomal degradation, thereby suppressing antiviral IFN-I responses. Notably, the F-box domain of fbxo3 is not required for the interaction with irf3/irf7 or for inhibiting their transactivity. Co-immunoprecipitation, ubiquitination assays specifying K27-linkage, zebrafish knockout (fbxo3 disruption), survival assay upon viral challenge, overexpression studies Journal of immunology High 32859728
2020 FBXO3 (as an SCF E3 ubiquitin ligase substrate receptor) binds and ubiquitylates C21ORF2, targeting it for proteasomal degradation. C21ORF2 stabilizes the kinase NEK1; consequently, loss of FBXO3 stabilizes both C21ORF2 and NEK1. Conversely, NEK1-mediated phosphorylation of C21ORF2 attenuates its interaction with FBXO3, stabilizing C21ORF2. The ALS-associated V58L mutant of C21ORF2 is hyperphosphorylated by NEK1 and therefore escapes FBXO3-mediated ubiquitylation, leading to its accumulation along with NEK1. Co-immunoprecipitation, ubiquitylation assays, proteasomal degradation assays, phosphorylation analysis, ALS mutant characterization, motor neuron differentiation from mouse ESCs with neurite outgrowth readout iScience High 32891887
2021 Spinal Fbxo3 mediates ubiquitination and degradation of CARM1 (coactivator-associated arginine methyltransferase 1) after nerve injury. Reduced CARM1 decreases H3R17me2 at K+ channel promoters, causing epigenetic silencing of K+ channel genes and contributing to neuropathic allodynia. Intrathecal BC-1215 (Fbxo3 inhibitor) prevented CARM1 ubiquitination and blocked K+ channel gene silencing. Spinal nerve ligation rat model, siRNA knockdown, CARM1 inhibitor, intrathecal BC-1215, ChIP assay for H3R17me2 at K+ channel promoters, behavioral allodynia assays Neurotherapeutics High 33415686
2022 FBXO3 promotes ubiquitination and proteasomal degradation of HIPK2 (Homeodomain-Interacting Protein Kinase 2) in the context of cerebral ischemia/reperfusion injury, contributing to neuroinflammation. siRNA knockdown of FBXO3 and the FBXO3 inhibitor BC-1215 both preserved HIPK2 protein levels and reduced neuronal damage and inflammatory cytokine production in vivo and in vitro. MCAO/R rat model, OGD/R neuronal cell model, siRNA knockdown, BC-1215 pharmacological inhibition, Western blot for HIPK2, inflammatory cytokine measurement International journal of molecular sciences Medium 36362432
2023 FBXO3 stabilizes USP4 by disrupting the interaction between USP4 and DNPEP (aspartyl aminopeptidase), protecting USP4 from DNPEP-mediated degradation. Stabilized USP4 in turn stabilizes Twist1, promoting breast cancer cell migration and tumor metastasis. This function is independent of FBXO3's E3 ligase activity. Additionally, PI3K (p110αH1047R) facilitates FBXO3 phosphorylation and stabilization in an ERK1-dependent manner. Co-immunoprecipitation (FBXO3-USP4, USP4-DNPEP interactions), siRNA knockdown, mouse metastasis models, Western blot for protein stability, phosphorylation analysis PLoS biology High 38134227
2024 Ginsenoside Rg1 reduces FBXO3 protein stability in an m6A-YTHDF1-dependent manner. FBXO3 interacts with PGC-1α and promotes its ubiquitination, targeting PGC-1α for degradation. Reduction of FBXO3 by Rg1 activates the PGC-1α/Nrf2 signaling pathway, improving mitochondrial function in sepsis-induced acute lung injury. Co-IP confirmed FBXO3–YTHDF1 and FBXO3–PGC-1α interactions. Co-immunoprecipitation (FBXO3-YTHDF1 and FBXO3-PGC-1α), MeRIP assay (m6A modification of FBXO3 mRNA), RIP assay, ubiquitination assay, CLP rat model, LPS cell model The AAPS journal Medium 38622374
2025 The cryo-EM structure of the human SCF(FBXO3) complex (CUL1-RBX1-SKP1-FBXO3) was solved at 3.70 Å nominal resolution. The F-box domain of FBXO3 associates with SKP1 via extensive hydrophobic interactions and interacts with the N-terminal region of CUL1 via hydrophobic interactions. The weak density for the RBX1 globular region near the FBXO3 ApaG domain suggests an unmodified closed conformation, and CUL1 neddylation is likely required for high E3 activity. Cryo-EM structure determination at 3.70 Å Proteins Medium 39921442
2025 TBK1-dependent phosphorylation of FBXO3 facilitates its interaction with TMEM192, promoting TMEM192 ubiquitination and subsequent recognition by the autophagy receptor TAX1BP1, orchestrating lysophagic flux following lysosomal damage. Perturbing this TBK1-SCFFBXO3-TMEM192-TAX1BP1 axis significantly reduces lysophagic flux and causes accumulation of damaged lysosomes. Genetic perturbation of pathway components, phosphorylation assays, ubiquitination assays, lysophagy flux assays, co-immunoprecipitation Autophagy Medium 40083080
2026 FBXO3 interacts with DUSP9 and promotes its ubiquitination, activating the MAPK pathway and supporting leukemia stem cell (LSC) maintenance and TKI resistance in CML. FBXO3 deficiency induced apoptosis and reduced proliferation of CML LSCs in vitro and in vivo, with minimal effects on normal hematopoietic stem cells. DUSP9 knockdown partially reversed the effects of FBXO3 deficiency. Co-immunoprecipitation (FBXO3-DUSP9), ubiquitination assays, siRNA/genetic knockdown, in vitro and in vivo CML LSC models, scRNA-seq for expression context, pharmacological FBXO3 inhibition Cell reports. Medicine High 41850237
2024 A molecular glue degrader (dHTC3) was found to selectively dimerize the first bromodomain of BRD4 to SCF(FBXO3), identifying FBXO3 as an E3 ligase accessible for chemical rewiring via proximity pharmacology. High-throughput SuFEx chemistry screening, degrader activity assays, biochemical characterization of BRD4-dHTC3-SCFFBXO3 ternary complex bioRxivpreprint Low bio_10.1101_2024.09.30.615685

Source papers

Stage 0 corpus · 61 papers · ranked by NIH iCite citations
Year Title Journal Citations PMID
2001 Fba, a novel fibronectin-binding protein from Streptococcus pyogenes, promotes bacterial entry into epithelial cells, and the fba gene is positively transcribed under the Mga regulator. Molecular microbiology 147 11679068
2014 Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH. Journal of virology 68 24403578
2013 Targeting F box protein Fbxo3 to control cytokine-driven inflammation. Journal of immunology (Baltimore, Md. : 1950) 63 24123678
2016 Genome-wide analysis of the fructose 1,6-bisphosphate aldolase (FBA) gene family and functional characterization of FBA7 in tomato. Plant physiology and biochemistry : PPB 58 27474933
2014 Fructose-1,6-bisphosphate aldolase (FBA)-a conserved glycolytic enzyme with virulence functions in bacteria: 'ill met by moonlight'. Biochemical Society transactions 44 25399608
2018 Escher-FBA: a web application for interactive flux balance analysis. BMC systems biology 42 30257674
2016 From DNA to FBA: How to Build Your Own Genome-Scale Metabolic Model. Frontiers in microbiology 37 27379044
2015 Elucidating temporal resource allocation and diurnal dynamics in phototrophic metabolism using conditional FBA. Scientific reports 37 26496972
2015 Fbxo3-Dependent Fbxl2 Ubiquitination Mediates Neuropathic Allodynia through the TRAF2/TNIK/GluR1 Cascade. The Journal of neuroscience : the official journal of the Society for Neuroscience 35 26674878
2020 FBA reveals guanylate kinase as a potential target for antiviral therapies against SARS-CoV-2. Bioinformatics (Oxford, England) 34 33381848
2020 Zebrafish F-box Protein fbxo3 Negatively Regulates Antiviral Response through Promoting K27-Linked Polyubiquitination of the Transcription Factors irf3 and irf7. Journal of immunology (Baltimore, Md. : 1950) 30 32859728
2016 Spinal Fbxo3-Dependent Fbxl2 Ubiquitination of Active Zone Protein RIM1α Mediates Neuropathic Allodynia through CaV2.2 Activation. The Journal of neuroscience : the official journal of the Society for Neuroscience 30 27629721
2016 FBXO3 Protein Promotes Ubiquitylation and Transcriptional Activity of AIRE (Autoimmune Regulator). The Journal of biological chemistry 27 27365398
2009 FBA-TPQ, a novel marine-derived compound as experimental therapy for prostate cancer. Investigational new drugs 27 19274441
2020 An Amyotrophic Lateral Sclerosis-Associated Mutant of C21ORF2 Is Stabilized by NEK1-Mediated Hyperphosphorylation and the Inability to Bind FBXO3. iScience 26 32891887
2015 F-box protein Fbxo3 targets Smurf1 ubiquitin ligase for ubiquitination and degradation. Biochemical and biophysical research communications 24 25721664
2023 FBXO3 stabilizes USP4 and Twist1 to promote PI3K-mediated breast cancer metastasis. PLoS biology 22 38134227
1968 Nutrition of Myxococcus xanthus FBa and some of its auxotrophic mutants. Journal of bacteriology 22 4868349
2012 Preclinical evaluation of anticancer efficacy and pharmacological properties of FBA-TPQ, a novel synthetic makaluvamine analog. Marine drugs 19 22822362
2022 E3 Ubiquitin Ligase FBXO3 Drives Neuroinflammation to Aggravate Cerebral Ischemia/Reperfusion Injury. International journal of molecular sciences 18 36362432
2019 Targeting F-Box Protein Fbxo3 Attenuates Lung Injury Induced by Ischemia-Reperfusion in Rats. Frontiers in pharmacology 18 31178737
2019 Genome-Wide Identification and Characterization of FBA Gene Family in Polyploid Crop Brassica napus. International journal of molecular sciences 18 31731804
2016 OM-FBA: Integrate Transcriptomics Data with Flux Balance Analysis to Decipher the Cell Metabolism. PloS one 18 27100883
2021 Genome-wide characterization, evolution, and expression profiling of FBA gene family in response to light treatments and abiotic stress in Nicotiana tabacum. Plant signaling & behavior 17 34120568
2017 Population FBA predicts metabolic phenotypes in yeast. PLoS computational biology 16 28886026
2022 miR-219a-5p inhibits the pyroptosis in knee osteoarthritis by inactivating the NLRP3 signaling via targeting FBXO3. Environmental toxicology 15 35962723
2021 Synthetic conjugate peptide Fba-Met6 (MP12) induces complement-mediated resistance against disseminated Candida albicans. Vaccine 14 34127293
2021 Blocking the Spinal Fbxo3/CARM1/K+ Channel Epigenetic Silencing Pathway as a Strategy for Neuropathic Pain Relief. Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 12 33415686
2021 FBA: feature barcoding analysis for single cell RNA-Seq. Bioinformatics (Oxford, England) 12 33999185
2008 Incorporation of enzyme concentrations into FBA and identification of optimal metabolic pathways. BMC systems biology 12 18634554
2024 Liposomal Fba and Met6 peptide vaccination protects mice from disseminated candidiasis. mSphere 11 38904363
2023 Genome-Wide Analysis of the FBA Subfamily of the Poplar F-Box Gene Family and Its Role under Drought Stress. International journal of molecular sciences 10 36902250
2019 MiR-142-3p Attenuates Oxygen Glucose Deprivation/Reoxygenation-Induced Injury by Targeting FBXO3 in Human Neuroblastoma SH-SY5Y Cells. World neurosurgery 10 31863884
2016 Crystal structure and interaction studies of the human FBxo3 ApaG domain. The FEBS journal 10 27010866
2016 Assessment of FBA Based Gene Essentiality Analysis in Cancer with a Fast Context-Specific Network Reconstruction Method. PloS one 10 27145226
2021 Genome-Wide Characterization and Expression Analysis Provide Basis to the Biological Function of Cotton FBA Genes. Frontiers in plant science 9 34490001
2015 Resource allocation in metabolic networks: kinetic optimization and approximations by FBA. Biochemical Society transactions 9 26614660
2024 Ginsenoside Rg1 Alleviates Sepsis-Induced Acute Lung Injury by Reducing FBXO3 Stability in an m6A-Dependent Manner to Activate PGC-1α/Nrf2 Signaling Pathway. The AAPS journal 8 38622374
2024 Characterization of FBA genes in potato (Solanum tuberosum L.) and expression patterns in response to light spectrum and abiotic stress. Frontiers in genetics 8 38686025
2021 Understanding FBA Solutions under Multiple Nutrient Limitations. Metabolites 7 33919383
2011 Functional identification of a novel F-box/FBA gene in tomato. Physiologia plantarum 7 22084837
2025 NEXT-FBA: A hybrid stoichiometric/data-driven approach to improve intracellular flux predictions. Metabolic engineering 6 40118205
2024 Identification of the fructose 1,6-bisphosphate aldolase (FBA) family genes in maize and analysis of the phosphorylation regulation of ZmFBA8. Plant science : an international journal of experimental plant biology 6 39481761
2023 FBA-PRCC. Partial Rank Correlation Coefficient (PRCC) Global Sensitivity Analysis (GSA) in Application to Constraint-Based Models. Biomolecules 3 36979435
2025 Structural Insight Into the SKP1-CUL1-FBXO3-RBX1 Complex. Proteins 2 39921442
2024 Overexpression of the FBA and TPI genes promotes high production of HDMF in Zygosaccharomyces rouxii. Frontiers in microbiology 2 38577687
2022 Genome-wide identification and characterization of the tomato F-box associated (FBA) protein family and expression analysis of their responsiveness to Phytophthora infestans. Gene 2 35182672
2025 The TBK1-SCFFBXO3-TMEM192-TAX1BP1 axis: a novel regulatory mechanism for lysophagy. Autophagy 1 40083080
2025 The FBA solution space kernel: introduction and illustrative examples. BMC bioinformatics 1 40676517
2025 Optimization-based framework with flux balance analysis (FBA) and metabolic pathway analysis (MPA) for identifying metabolic objective functions. PLoS computational biology 1 41144574
2023 The free bilamellar autograft (FBA) procedure: A comprehensive case series of an alternative surgical approach to reconstruction of large eyelid defects. Frontiers in surgery 1 36911616
2021 Knowledge extraction from literature and enzyme sequences complements FBA analysis in metabolic engineering. Biotechnology journal 1 34516717
2011 Do body-part concepts depend on the EBA/FBA? Cognitive neuroscience 1 24168535
2026 Spatial FBA reveals heterogeneous Warburg niches in renal tumors and lactate consumption in colorectal cancer. NPJ systems biology and applications 0 41593100
2026 FBXO3-mediated DUSP9 ubiquitination promotes leukemia stem cell maintenance and tyrosine kinase inhibitor resistance in chronic myeloid leukemia. Cell reports. Medicine 0 41850237
2026 Oral administration of recombinant Lactobacillus plantarum co expressing FimB, CnaA, NetB and FBA antigens targeting chicken dendritic cells provides effective protection against necrotizing enteritis in broilers. Poultry science 0 41950756
2025 Genome-Wide Identification and Expression Analysis of the Fructose-1,6-Bisphosphate Aldolase (FBA) Gene Family in Sweet Potato and Its Two Diploid Relatives. International journal of molecular sciences 0 40806478
2025 PET-FBA: A lightweight enzyme allocation and thermodynamics-constrained flux analysis approach to explore Escherichia coli metabolic adaptation to intracellular acidification. Metabolic engineering 0 41386341
2024 Mutation of negative regulatory gene CEHC1 encoding an FBXO3 protein results in normoxic expression of HYDA genes in Chlamydomonas reinhardtii. bioRxiv : the preprint server for biology 0 38586028
2022 Multi-Objective Optimization of Microalgae Metabolism: An Evolutive Algorithm Based on FBA. Metabolites 0 35888727
2021 Global connectivity in genome-scale metabolic networks revealed by comprehensive FBA-based pathway analysis. BMC microbiology 0 34696732

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