FAM124B

FAM124B is a predominantly nuclear protein that functions as a component of a CHD7- and CHD8-containing chromatin remodeling complex (PMID:23285124). It binds directly to the CHD8 subunit and co-immunoprecipitates with the assembled CHD7/CHD8 complex, and it is expressed broadly across embryonic and adult tissues (PMID:23285124). Beyond this complex association, its precise molecular activity within the chromatin remodeling machinery has not been characterized in the available corpus.

1. 2012 Medium

   Establishing whether FAM124B has any defined molecular context, this work identified it as a direct binding partner of CHD8 and a member of a CHD7/CHD8 chromatin remodeling complex, placing an uncharacterized protein into a defined nuclear assembly.

   Evidence SILAC mass spectrometry, reciprocal co-immunoprecipitation, direct yeast two-hybrid, and immunofluorescence/subcellular fractionation

   PMID:23285124

   Open questions at the time

   • The functional contribution of FAM124B to the CHD7/CHD8 complex's remodeling activity is undefined
   • No catalytic or structural role assigned within the complex
   • Target loci or chromatin states regulated by the complex via FAM124B are unknown
2. 2024 Low

   Probing a cellular phenotype for FAM124B, loss-of-function in endothelial cells reduced proliferation, providing the first functional readout tied to the gene.

   Evidence In vitro knockdown/inhibition proliferation assay in HUVECs

   PMID:39384883

   Open questions at the time

   • Single in vitro knockdown without mechanistic pathway placement
   • No link established between the endothelial proliferation phenotype and the CHD7/CHD8 complex role
   • Not independently confirmed

• The molecular function FAM124B performs within the CHD7/CHD8 complex and how this connects to its cellular roles remains unresolved.
• No defined biochemical activity for FAM124B
• No connection drawn between chromatin complex membership and the endothelial proliferation phenotype