- Length
- 340 aa
- Mass
- 35.8 kDa
- Annotated
- 2026-04-28
50 papers in source corpus
14 papers cited in narrative
14 extracted findings
Mechanistic narrative
Synthesis pass · prose summary of the discoveries below
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Mechanism profile
Synthesis pass · controlled-vocabulary classification · explore literature graph →
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Evidence
Reading pass · 14 per-paper findings extracted from the source corpus
| Year | Finding | Method | Journal | Conf | PMIDs |
|---|---|---|---|---|---|
| 2011 | Ephrin-B3 (EFNB3) acts as an essential component of the Reelin signaling pathway for neuronal migration: Reelin binds directly to the extracellular domain of ephrin-Bs including EFNB3, which associate at the neuronal membrane with VLDLR and ApoER2 receptors; clustering of ephrin-Bs recruits and promotes phosphorylation of Dab1, which is required for Reelin signaling; loss of ephrin-B function severely impairs Reelin-induced Dab1 phosphorylation; activation of ephrin-Bs can rescue reeler neuronal migration defects in the absence of Reelin. | Co-immunoprecipitation, genetic compound mutants (Reln+/-;Efnb3-/- mice), triple ephrin-B1/B2/B3 knockouts, rescue experiments, Dab1 phosphorylation assays | Nature | High | 21460838 |
| 2016 | EFNB3 knockout in female mice leads to increased blood pressure and augmented vascular smooth muscle cell (VSMC) contractility; EFNB3 deletion in VSMCs results in reduced myosin light chain kinase phosphorylation; estrogen augments VSMC contractility via a nongenomic GPER pathway while testosterone reduces it in the absence of EFNB3; these effects are absent in wild-type VSMCs, indicating EFNB3 normally buffers sex hormone effects on VSMC contractility. | Efnb3 gene knockout mice, VSMC contractility assays, GPER knockdown assays, ovariectomy experiments, myosin light chain kinase phosphorylation assays | American journal of physiology. Heart and circulatory physiology | High | 26851246 |
| 2016 | GRIP1, a glutamate receptor interacting protein in the EFNB3 reverse signaling pathway, regulates VSMC contractility; siRNA knockdown of GRIP1 in mouse VSMCs increases contractility, and Efnb3 KO mice show enhanced carotid artery constriction by echocardiography. | siRNA knockdown of GRIP1 in VSMCs, Efnb3 KO mouse echocardiography, in vitro contractility assays | Scientific reports | Medium | 27941904 |
| 2003 | Mouse EFNB3 expressed on T cells acts as a costimulatory ligand: solid-phase EFNB3-Fc in the presence of suboptimal anti-CD3 crosslinking enhances T-cell proliferation, activation marker expression, IFN-γ production, and cytotoxic T-cell activity; TCR and EFNB3 receptors co-congregate into lipid rafts upon crosslinking; EFNB3R costimulation augments p38 and p44/42 MAPK activation; the effect is insensitive to cyclosporin A, resembling CD28 costimulation. | Solid-phase EFNB3-Fc stimulation assays, proliferation assays, cytokine measurements, lipid raft co-localization, MAPK activation assays, cyclosporin A pharmacological treatment | The Journal of biological chemistry | Medium | 13679370 |
| 2008 | Crystal structures of Nipah and Hendra virus attachment glycoproteins in complex with human EFNB2 revealed that EFNB3 (as analogous receptor) mediates viral attachment via an extensive protein-protein interface involving a crucial phenylalanine side chain on EFNB2/B3 that inserts into a hydrophobic pocket on the viral attachment protein; this is mechanistically distinct from sialic acid-based paramyxovirus attachment. | X-ray crystallography of viral attachment glycoprotein–EFNB2 complex | Nature structural & molecular biology | High | 18488039 |
| 2024 | Structure-guided mutagenesis of NiV receptor-binding protein identified two regions (including residues N557 and Y581) critical for EFNB3 binding; NiV interaction with Y120 of EFNB3 is important for EFNB3 receptor usage; substitution of these residues severely impairs EFNB3-mediated entry by NiV pseudotyped viral particles. | Structure-guided mutagenesis, EFNB3 binding assays, pseudotyped viral particle entry assays | Journal of virology | High | 38426726 |
| 2016 | Contrary to earlier claims, compound mutant Efnb3-/-;Reln+/- mice and triple Efnb1;2;3-/- mice do not show defects of cortical layering or elevated Dab1 levels, indicating that while protein-protein interactions between ephrin-Bs and Reelin have been confirmed, they do not affect the radial positioning of migrating neocortical pyramidal neurons. | Efnb3-/-;Reln+/- compound mutants, triple Efnb1;2;3-/- knockout mice, cortical layering analysis, Dab1 protein level measurement | Nature | Medium | 27882975 |
| 2021 | Ephrin-B3/EphBs forward signaling in the spinal cord regulates nociception: Efnb3 knockout mice show increased thermal and mechanical thresholds and aberrant thin myelin sheaths; PLP (proteolipid protein) expression is reduced in the L4-6 spinal cord of Efnb3-/- mice; intrathecal PLP siRNA increases pain thresholds in wild-type mice; AAV9-PLP overexpression rescues pain hypersensitivity in Efnb3-/- mice; Efnb3lacz mice (forward signaling intact, reverse signaling absent) show normal pain behavior, indicating the reverse signaling is responsible. | Efnb3-/- and Efnb3lacz conditional knock-in mice, behavioral pain assays, western blot, immunofluorescence, intrathecal siRNA, AAV9 gene delivery | Fundamental & clinical pharmacology | Medium | 34904278 |
| 2022 | Continuous activation of EFNB3 reverse signaling in benign prostate fibroblasts induces a cancer-associated fibroblast (CAF) phenotype and pro-tumorigenic secretome; this effect is mediated through Src family kinase (SFK) activation; SFK inhibition suppresses EFNB3-induced α-SMA and Tenascin-C expression in vitro. | Stable EFNB3 overexpression in BHPrS1 cells, SFK inhibitor treatment, CAF marker expression assays, in vitro and in vivo tumorigenicity assays | Cancers | Medium | 35565468 |
| 2023 | Ephrin B3 promotes intestinal epithelial barrier dysfunction and inflammation in colitis via two pathways: (1) inactivation of the ITGB4/AKT signaling pathway promoting epithelial barrier dysfunction; (2) activation of the Gremlin-1/NF-κB signaling pathway increasing inflammatory factor release; Efnb3-/- mice showed reduced DSS-induced colitis and reduced susceptibility to colitis-associated colorectal cancer. | Efnb3-/- mice, DSS-induced colitis model, AOM/DSS colorectal cancer model, label-free quantitative proteomics, western blotting | Biochemical pharmacology | Medium | 38142837 |
| 2025 | Ephrin B3 drives tumorigenesis in cutaneous squamous cell carcinoma via regulation of CK19 (cytokeratin 19), which in turn positively regulates the NOTCH1 signaling pathway; Efnb3 deficiency also mitigates inflammatory responses through the MAPK pathway in acute skin inflammation. | Efnb3-/- mice in DMBA/TPA carcinogenesis model, EFNB3 knockdown in A431 cells, label-free proteomics, Reactome pathway analysis | Medical oncology | Medium | 40711642 |
| 2019 | LHX1 transcription factor regulates the expression of Eph/ephrin family guidance receptors including those responsive to ephrin-B3 in POA-derived cortical interneurons, thereby modulating their directional migration and layer distribution. | Conditional LHX1 knockout in POA-derived interneurons, live imaging of interneuron migration, layer distribution analysis | Cerebral cortex | Low | 29912395 |
| 1997 | EFNB3 (originally designated EPLG8/LERK-8) was cloned and mapped to human chromosome 17p11.2-p13.1; it encodes a transmembrane protein related to ephrin-B2 and another B-class ephrin, expressed predominantly in brain and expressed at highest levels in forebrain subregions. | cDNA library screening, chromosomal mapping by PCR of somatic cell hybrid panels, Northern blot tissue expression analysis | Genomics | Medium | 9126477 |
| 2025 | A frameshift variant (2-bp duplication, c.643_644dup) in EFNB3 causes autosomal recessive congenital mirror movement disorder in Weimaraner dogs, resembling the phenotype of Efnb3-/- knockout mice, establishing EFNB3 as necessary for normal spinal midline barrier formation during neurodevelopment. | Linkage and autozygosity mapping, whole-genome sequencing of 3 affected and 1489 control dogs, pathological investigations | Movement disorders | Medium | 40401490 |
Source papers
Stage 0 corpus · 50 papers · ranked by NIH iCite citations
| Year | Title | Journal | Citations | PMID |
|---|---|---|---|---|
| 2008 | Structural basis of Nipah and Hendra virus attachment to their cell-surface receptor ephrin-B2. | Nature structural & molecular biology | 197 | 18488039 |
| 2011 | Ephrin Bs are essential components of the Reelin pathway to regulate neuronal migration. | Nature | 135 | 21460838 |
| 2016 | Genome-wide DNA Methylation Changes in a Mouse Model of Infection-Mediated Neurodevelopmental Disorders. | Biological psychiatry | 121 | 27769567 |
| 2000 | Implications of EPHB6, EFNB2, and EFNB3 expressions in human neuroblastoma. | Proceedings of the National Academy of Sciences of the United States of America | 86 | 10984508 |
| 1999 | Coexpression of transcripts encoding EPHB receptor protein tyrosine kinases and their ephrin-B ligands in human small cell lung carcinoma. | Clinical cancer research : an official journal of the American Association for Cancer Research | 79 | 10037197 |
| 2011 | Decreased expression of axon-guidance receptors in the anterior cingulate cortex in autism. | Molecular autism | 75 | 21859478 |
| 1999 | High-level expression of EPHB6, EFNB2, and EFNB3 is associated with low tumor stage and high TrkA expression in human neuroblastomas. | Clinical cancer research : an official journal of the American Association for Cancer Research | 74 | 10389937 |
| 2010 | De novo expression of EphA2 in osteosarcoma modulates activation of the mitogenic signalling pathway. | Histopathology | 58 | 21166698 |
| 2014 | Transcriptional regulation by FOXP1, FOXP2, and FOXP4 dimerization. | Journal of molecular neuroscience : MN | 52 | 25027557 |
| 2003 | Mouse ephrinB3 augments T-cell signaling and responses to T-cell receptor ligation. | The Journal of biological chemistry | 48 | 13679370 |
| 2019 | Pituitary Transcriptomic Study Reveals the Differential Regulation of lncRNAs and mRNAs Related to Prolificacy in Different FecB Genotyping Sheep. | Genes | 46 | 30781725 |
| 2004 | Favorable neuroblastoma genes and molecular therapeutics of neuroblastoma. | Clinical cancer research : an official journal of the American Association for Cancer Research | 46 | 15355914 |
| 2014 | Identification of molecular pathways facilitating glioma cell invasion in situ. | PloS one | 40 | 25365423 |
| 2019 | Sonic hedgehog pathway activation is associated with cetuximab resistance and EPHB3 receptor induction in colorectal cancer. | Theranostics | 34 | 31149041 |
| 2022 | Runs of Homozygosity Revealed Reproductive Traits of Hu Sheep. | Genes | 32 | 36292733 |
| 2012 | Cancer stem cells from a rare form of glioblastoma multiforme involving the neurogenic ventricular wall. | Cancer cell international | 24 | 22995409 |
| 2001 | Association among EPHB2, TrkA, and MYCN expression in low-stage neuroblastomas. | Medical and pediatric oncology | 24 | 11464911 |
| 2012 | 790 Kb microduplication in chromosome band 17p13.1 associated with intellectual disability, afebrile seizures, dysmorphic features, diabetes, and hypothyroidism. | European journal of medical genetics | 20 | 22365944 |
| 2022 | Ephrin B Activate Src Family Kinases in Fibroblasts Inducing Stromal Remodeling in Prostate Cancer. | Cancers | 17 | 35565468 |
| 2019 | The Transcription Factor LHX1 Regulates the Survival and Directed Migration of POA-derived Cortical Interneurons. | Cerebral cortex (New York, N.Y. : 1991) | 17 | 29912395 |
| 2006 | Comparative integromics on Ephrin family. | Oncology reports | 17 | 16596216 |
| 2016 | Estrogen and testosterone in concert with EFNB3 regulate vascular smooth muscle cell contractility and blood pressure. | American journal of physiology. Heart and circulatory physiology | 16 | 26851246 |
| 2010 | 17p13.1 microdeletion involving the TP53 gene in a boy presenting with mental retardation but no tumor. | American journal of medical genetics. Part A | 16 | 20425836 |
| 2007 | De novo identification of MIZ-1 (ZBTB17) encoding a MYC-interacting zinc-finger protein as a new favorable neuroblastoma gene. | Clinical cancer research : an official journal of the American Association for Cancer Research | 16 | 17947461 |
| 1997 | cDNA cloning, chromosomal localization, and expression pattern of EPLG8, a new member of the EPLG gene family encoding ligands of EPH-related protein-tyrosine kinase receptors. | Genomics | 16 | 9126477 |
| 2013 | Destabilization of MYC/MYCN by the mitochondrial inhibitors, metaiodobenzylguanidine, metformin and phenformin. | International journal of molecular medicine | 15 | 24190252 |
| 2009 | High level MycN expression in non-MYCN amplified neuroblastoma is induced by the combination treatment nutlin-3 and doxorubicin and enhances chemosensitivity. | Oncology reports | 14 | 19885598 |
| 2016 | The role of GRIP1 and ephrin B3 in blood pressure control and vascular smooth muscle cell contractility. | Scientific reports | 13 | 27941904 |
| 2016 | Ephrin Bs and canonical Reelin signalling. | Nature | 12 | 27882975 |
| 2014 | Neurodevelopmental delays and macrocephaly in 17p13.1 microduplication syndrome. | American journal of medical genetics. Part A | 12 | 25123844 |
| 2022 | Glycoprotein attachment with host cell surface receptor ephrin B2 and B3 in mediating entry of nipah and hendra virus: a computational investigation. | Journal of chemical sciences (Bangalore, India) | 9 | 36465097 |
| 2017 | Evidence from single nucleotide polymorphism analyses of ADVANCE study demonstrates EFNB3 as a hypertension risk gene. | Scientific reports | 8 | 28272517 |
| 2021 | Investigation of Key Signaling Pathways Associating miR-204 and Common Retinopathies. | BioMed research international | 7 | 34646884 |
| 2020 | Transcriptomic profile of Pea3 family members reveal regulatory codes for axon outgrowth and neuronal connection specificity. | Scientific reports | 7 | 33097800 |
| 2015 | Genome-wide association pathway analysis to identify candidate single nucleotide polymorphisms and molecular pathways for gastric adenocarcinoma. | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | 6 | 25687184 |
| 2025 | Interpretable machine learning-guided single-cell mapping deciphers multi-lineage pancreatic dysregulation in type 2 diabetes. | Cardiovascular diabetology | 4 | 40707916 |
| 2024 | Structure-guided mutagenesis of Henipavirus receptor-binding proteins reveals molecular determinants of receptor usage and antibody-binding epitopes. | Journal of virology | 4 | 38426726 |
| 2023 | Evaluation of the interaction between potent small molecules against the Nipah virus Glycoprotein in Malaysia and Bangladesh strains, accompanied by the human Ephrin-B2 and Ephrin-B3 receptors; a simulation approach. | Molecular diversity | 4 | 36808582 |
| 2018 | Analysis of the association of EPHB6, EFNB1 and EFNB3 variants with hypertension risks in males with hypogonadism. | Scientific reports | 4 | 30262919 |
| 2025 | The alternatively spliced diacylglycerol kinase gamma-Δ exon13 transcript generated under hypoxia promotes glioblastoma progression. | Oncology research | 3 | 40296900 |
| 2025 | EFNB3 Frameshift Variant in Weimaraner Dogs with a Condition Resembling a Congenital Mirror Movement Disorder. | Movement disorders : official journal of the Movement Disorder Society | 3 | 40401490 |
| 2023 | Integrated analysis of RNA-seq in hepatocellular carcinoma reveals competing endogenous RNA network composed of circRNA, lncRNA, and mRNA. | Medicine | 3 | 36827016 |
| 2023 | Ephrin B3 exacerbates colitis and colitis-associated colorectal cancer. | Biochemical pharmacology | 2 | 38142837 |
| 2021 | Activation of spinal ephrin-B3/EphBs signaling induces hyperalgesia through a PLP-mediated mechanism. | Fundamental & clinical pharmacology | 2 | 34904278 |
| 2026 | Epigenetic mediators of diet and lifestyle and insulin resistance. | Genes & nutrition | 0 | 41580603 |
| 2026 | In silico characterization of G protein-host receptor interactions in Bangladesh Nipah virus mutants toward therapeutic target identification. | In silico pharmacology | 0 | 41613644 |
| 2025 | Ephrin B3 drives tumorigenesis and inflammation in cutaneous squamous cell carcinoma. | Medical oncology (Northwood, London, England) | 0 | 40711642 |
| 2025 | Molecular Encoding of Ischemic Stroke and its Resolution after Human Neural Stem Cell Therapy by Extracellular Vesicles. | MedComm | 0 | 41164797 |
| 2025 | Germline Variant Call Accuracy in Whole Genome Sequence Data from Canine Formalin-Fixed Paraffin-Embedded Tissue Samples. | Genes | 0 | 41300821 |
| 2023 | Structure guided mutagenesis of Henipavirus Receptor Binding Proteins reveals molecular determinants of receptor usage and antibody binding epitopes. | bioRxiv : the preprint server for biology | 0 | 38045373 |