Ephrin-A5 · UniProt P52803
EFNA5 (ephrin-A5/LERK-7) is a GPI-anchored membrane ligand for Eph-family receptor tyrosine kinases that drives contact-dependent cell patterning and repulsive signaling across neural, lens, and reproductive tissues (PMID:9245480). It binds the Eph receptors hek and elk and, in soluble form, stimulates phosphorylation of the eck receptor, establishing its activity as a receptor-activating ligand (PMID:9245480). In the developing lens, EFNA5 acts through EphA2 to align elongating fiber cells at the equator and organize Y-suture and radial column patterning, with loss of EFNA5 disrupting these morphologies (PMID:29800803). In the nervous system, EFNA5 mediates axonal repulsion of layer 2/3 callosal projection neurons through EphA receptors, with target sensitivity set by receptor (EphA6) abundance. EFNA5 also functions as a tumor suppressor, inhibiting proliferation, migration, and invasion in colorectal, lung, and hepatoma cells, the last associated with downregulation of EMT markers and EGFR (PMID:32036212, PMID:37333453, PMID:39424684), and its expression is constrained post-transcriptionally by miRNAs (miR-645, miR-1289, miR-182-5p) and by PIWIL1/piRNA-mediated mRNA degradation (PMID:33389678, PMID:32036212, PMID:37333453, PMID:37530910). As an oocyte-derived factor, recombinant EFNA5 improves oocyte developmental competence by activating NRF2 antioxidant signaling and suppressing NF-κB-driven inflammation during in vitro maturation (PMID:41462676). The structural and biochemical details of EFNA5-Eph receptor engagement beyond ligand binding and receptor phosphorylation have not been characterized in the available corpus.
Establishing the genomic location of EFNA5 anchored the gene for subsequent functional and disease studies.
Evidence PCR somatic cell hybrid screening, FISH, and interspecific backcross Southern blot
Identifying EFNA5/LERK-7 as a GPI-anchored ligand that binds Eph receptors and triggers receptor phosphorylation defined its core molecular activity as an Eph receptor agonist.
Evidence Receptor-Fc binding to transfected cells, soluble ligand phosphorylation assay, and Northern blot tissue survey
Genetic loss-of-function in mice showed EFNA5 is required for lens fiber cell alignment and suture formation, assigning it a developmental patterning role through EphA2.
Evidence Efna5-null mice with in situ hybridization, fiber cell imaging, and AQP0 immunofluorescence
Human sequence variants and a translocation disrupting EFNA5 nominated it as a candidate for lens pathology and a Mendelian disorder, but without functional validation.
Evidence Exon sequencing in cataract patients with PolyPhen2 prediction; whole-genome mate-pair sequencing of a single translocation patient with pLI/DOMINO scoring
Demonstrating piRNA/PIWIL1-mediated degradation of EFNA5 mRNA revealed a post-transcriptional control axis whose loss elevates EFNA5 and drives pancreatic cancer growth.
Evidence RNA pulldown, in vitro proliferation, and xenograft assays in pancreatic cancer cells
Identification of multiple miRNA and lncRNA regulatory axes (miR-645, miR-1289/LINC00607, EGR1→miR-182-5p) placing EFNA5 as a functional effector clarified how its expression is tuned in cancer and neovascularization.
Evidence Luciferase reporter, RNA pulldown, RIP, ChIP, and gain/loss-of-function with migration/invasion and ROP mouse readouts
PMID:32036212 PMID:37333453 PMID:37530910
EFNA5 overexpression suppressing hepatoma EMT and EGFR levels extended its tumor-suppressor role to liver cancer.
Evidence Plasmid overexpression with proliferation/invasion assays, EMT/EGFR Western blot, and patient IHC
Showing recombinant EFNA5 improves oocyte competence via NRF2 and NF-κB modulation expanded its role to reproductive biology and redox/inflammatory signaling.
Evidence Cross-species transcriptomics, recombinant EFNA5 supplementation in ovine IVM, ROS and apoptosis assays, RNA-seq, and NRF2/NF-κB Western blot
Genetic epistasis showed Mef2c sets EphA6 levels to control axonal sensitivity to EFNA5 repulsion, defining EFNA5 as a repulsive cue in callosal axon targeting.
Evidence Conditional Mef2c knockout mice with EphA-ephrinA manipulation and axon tracing (preprint)
| Year | Finding | Method | Journal | Conf | PMIDs |
|---|---|---|---|---|---|
| 1997 | LERK-7 (EFNA5) encodes a 228-amino acid GPI-anchored membrane protein that binds soluble forms of Eph-family receptors hek and elk when transfected into CV1/EBNA cells, and a soluble form of LERK-7 induces phosphorylation of the eck receptor expressed in a human duodenum adenocarcinoma cell line. LERK-7 is developmentally regulated in the brain, with highest expression in adult brain, heart, spleen, and ovary and fetal brain, lung, and kidney. | Receptor-binding assay (soluble receptor-Fc fusion proteins binding to transfected cells), phosphorylation assay (soluble LERK-7 stimulating eck phosphorylation in cell line), Northern blot for tissue distribution | Cytokine | Medium | 9245480 |
| 1996 | The gene encoding LERK-7 (EPLG7/EFNA5) maps to human chromosome 5q21 and mouse chromosome 17, established by PCR-based somatic cell hybrid screening and fluorescence in situ hybridization. | PCR-based somatic cell hybrid panel screening; fluorescence in situ hybridization (FISH); Southern blot of interspecific backcross mice | Genomics | High | 8661153 |
| 2018 | EFNA5 (and its receptor EPHA2) participate in lens fiber cell pattern formation. Mice lacking EFNA5 (Efna5-null) develop lenses with misaligned elongating fiber cells at the lens equator, loss of Y-suture pattern formation, and disrupted radial column patterning of hexagonal fiber cells throughout the cortex. In situ hybridization localized Efna5 transcripts to lens epithelial cells and nascent fiber cells at the lens equator. | Efna5-null mouse model; in situ hybridization; in vivo thymidine analog labeling; ex vivo imaging with membrane-targeted red fluorescent protein; immunofluorescent labeling of AQP0/MIP | Differentiation; research in biological diversity | High | 29800803 |
| 2014 | Three single nucleotide polymorphisms in EFNA5 (c.668C>T, c.102C>T, c.-27C>G) were identified in Chinese age-related cataract patients and were absent in normal controls; c.668C>T was predicted to be possibly damaging by PolyPhen2, linking EFNA5 sequence variation to lens pathology. | Sequencing of all EFNA5 exons in 140 ARC patients and 142 controls; PolyPhen2 computational analysis | Ophthalmic research | Low | 25300504 |
| 2018 | A de novo balanced chromosomal translocation disrupting EFNA5 was identified in a symptomatic patient by shallow whole-genome mate-pair sequencing and Sanger sequencing; EFNA5 displayed high pLI and DOMINO scores indicating likely intolerance to haploinsufficiency, nominating it as a candidate gene for a human Mendelian disorder. | Shallow whole-genome mate-pair sequencing (SGMPS); long-range PCR; Sanger sequencing; bioinformatic pLI/DOMINO scoring | Journal of medical genetics | Low | 30352868 |
| 2021 | piR-017061 cooperates with PIWIL1 to directly bind EFNA5 mRNA and facilitate its degradation in pancreatic cancer cells; loss of piR-017061 results in accumulation of EFNA5 protein, which facilitates pancreatic cancer cell growth in vitro and in vivo. | RNA pulldown (direct binding between piR-017061 and EFNA5 mRNA mediated by PIWIL1); in vitro cell proliferation assays; in vivo xenograft assays; qRT-PCR and Western blot for EFNA5 protein levels | Human cell | Medium | 33389678 |
| 2020 | miR-645 directly targets the 3'-UTR of EFNA5 mRNA and represses its expression; EFNA5 knockdown attenuates the suppressive effects of miR-645 knockdown on colorectal cancer cell migration and invasion, placing EFNA5 downstream of miR-645 as a tumor-suppressive target in colorectal cancer. | Luciferase reporter assay with EFNA5 3'-UTR; miR-645 overexpression/knockdown; EFNA5 siRNA knockdown; cell migration/invasion assays; in vivo tumor growth and metastasis assays; qRT-PCR correlation in patient tumors | Biomedicine & pharmacotherapy | Medium | 32036212 |
| 2023 | LINC00607 binds miR-1289 (validated by luciferase reporter, RNA pulldown, and RNA immunoprecipitation assays) and EFNA5 is a direct downstream target of miR-1289 (validated by luciferase reporter); EFNA5 overexpression inhibits NSCLC cell viability, proliferation, migration, and invasion, and EFNA5 knockdown antagonizes the tumor-suppressive effect of LINC00607 overexpression, placing EFNA5 as the functional effector of this lncRNA/miRNA axis. | Luciferase reporter assay; RNA pulldown assay; RNA immunoprecipitation (RIP); EFNA5 overexpression and knockdown; cell viability (MTT), colony formation, wound healing, and Transwell invasion assays | Open medicine (Warsaw, Poland) | Medium | 37333453 |
| 2023 | EGR1 binds the miR-182-5p promoter to inhibit miR-182-5p transcription (validated by ChIP and dual-luciferase assay), which in turn de-represses EFNA5 expression; EFNA5 overexpression promotes retinal neovascularization in a mouse ROP model and EGR1 knockdown-mediated suppression of neovascularization is reversed by miR-182-5p downregulation or EFNA5 overexpression. | Chromatin immunoprecipitation (ChIP) of EGR1 at miR-182-5p promoter; dual-luciferase promoter assay; ROP mouse model; in vitro tube formation assay; RT-qPCR and Western blot; gain- and loss-of-function experiments | Biochemical genetics | Medium | 37530910 |
| 2024 | Upregulation of EFNA5 in hepatoma cell lines (HepG2 and LM3) inhibits cell proliferation, invasion, and migration; mechanistically, EFNA5 overexpression downregulates EMT-related molecules and EGFR protein levels, suggesting EFNA5 suppresses hepatoma progression through inhibition of epithelial-to-mesenchymal transition. | EFNA5 plasmid overexpression; CCK-8 cell proliferation assay; Transwell and Boyden invasion/migration assays; Western blot for EMT markers and EGFR; IHC of patient samples | Discover oncology | Low | 39424684 |
| 2025 | EFNA5 is an oocyte-derived membrane ligand that is stably enriched in mammalian oocytes but reduced after in vitro maturation. Supplementation with recombinant EFNA5 during ovine IVM improves blastocyst formation, reduces apoptosis, promotes cumulus-oocyte complex expansion, reduces ROS accumulation, activates NRF2-dependent antioxidant signaling, and suppresses NF-κB-driven inflammation, as confirmed by RNA-seq and functional validation. | Cross-species transcriptomic analysis; recombinant EFNA5 supplementation in ovine IVM; blastocyst formation and total cell count assays; ROS measurement; Western blot for NRF2 and NF-κB pathway proteins; RNA-seq; DNA damage assays | Antioxidants (Basel, Switzerland) | Medium | 41462676 |
| 2025 | Mef2c downregulates EphA6 expression in layer 2/3 callosal projection neurons, thereby desensitizing their axons to EphrinA5 (EFNA5)-mediated repulsion at contralateral cortical targets; Mef2c deletion causes EphA6 upregulation and increased sensitivity to EFNA5 repulsion, resulting in mistargeting of S1-L2/3 CPN axons. | Conditional Mef2c knockout mice; EphA-EphrinA functional manipulation; axon tracing; genetic epistasis (Mef2c deletion + EphA6 modulation) | bioRxiv (preprint)preprint | Medium |
| Year | Title | Journal | Citations | PMID |
|---|---|---|---|---|
| 1999 | Ligand-independent recruitment of SRC-1 to estrogen receptor beta through phosphorylation of activation function AF-1. | Molecular cell | 379 | 10230404 |
| 1999 | The AF1 and AF2 domains of the androgen receptor interact with distinct regions of SRC1. | Molecular and cellular biology | 345 | 10567563 |
| 1998 | The steroid receptor coactivator-1 contains multiple receptor interacting and activation domains that cooperatively enhance the activation function 1 (AF1) and AF2 domains of steroid receptors. | The Journal of biological chemistry | 342 | 9575154 |
| 1993 | RARs and RXRs: evidence for two autonomous transactivation functions (AF-1 and AF-2) and heterodimerization in vivo. | The EMBO journal | 303 | 8389696 |
| 1997 | Stimulation of RAR alpha activation function AF-1 through binding to the general transcription factor TFIIH and phosphorylation by CDK7. | Cell | 264 | 9230306 |
| 2001 | A subfamily of RNA-binding DEAD-box proteins acts as an estrogen receptor alpha coactivator through the N-terminal activation domain (AF-1) with an RNA coactivator, SRA. | The EMBO journal | 229 | 11250900 |
| 2005 | Structure and function of steroid receptor AF1 transactivation domains: induction of active conformations. | The Biochemical journal | 157 | 16238547 |
| 2002 | The AF-1 activation-function of ERalpha may be dispensable to mediate the effect of estradiol on endothelial NO production in mice. | Proceedings of the National Academy of Sciences of the United States of America | 147 | 11854517 |
| 2001 | Synergism between ERalpha transactivation function 1 (AF-1) and AF-2 mediated by steroid receptor coactivator protein-1: requirement for the AF-1 alpha-helical core and for a direct interaction between the N- and C-terminal domains. | Molecular endocrinology (Baltimore, Md.) | 135 | 11682626 |
| 2003 | The AF-1 domain of the orphan nuclear receptor NOR-1 mediates trans-activation, coactivator recruitment, and activation by the purine anti-metabolite 6-mercaptopurine. | The Journal of biological chemistry | 133 | 12709428 |
| 2000 | p300 mediates functional synergism between AF-1 and AF-2 of estrogen receptor alpha and beta by interacting directly with the N-terminal A/B domains. | The Journal of biological chemistry | 126 | 10747867 |
| 2000 | Synergy between estrogen receptor alpha activation functions AF1 and AF2 mediated by transcription intermediary factor TIF2. | EMBO reports | 121 | 11265755 |
| 2001 | The conformation of the glucocorticoid receptor af1/tau1 domain induced by osmolyte binds co-regulatory proteins. | The Journal of biological chemistry | 120 | 11279138 |
| 1999 | Dominant activity of activation function 1 (AF-1) and differential stoichiometric requirements for AF-1 and -2 in the estrogen receptor alpha-beta heterodimeric complex. | Molecular and cellular biology | 116 | 10022879 |
| 2001 | Potentiation of estrogen receptor activation function 1 (AF-1) by Src/JNK through a serine 118-independent pathway. | Molecular endocrinology (Baltimore, Md.) | 104 | 11145737 |
| 2004 | Molecular mechanisms of androgen receptor-mediated gene regulation: structure-function analysis of the AF-1 domain. | Endocrine-related cancer | 102 | 15163303 |
| 1997 | Phosphorylation of activation functions AF-1 and AF-2 of RAR alpha and RAR gamma is indispensable for differentiation of F9 cells upon retinoic acid and cAMP treatment. | The EMBO journal | 99 | 9351827 |
| 2004 | Induced alpha-helix structure in AF1 of the androgen receptor upon binding transcription factor TFIIF. | Biochemistry | 86 | 15023052 |
| 2010 | Site-specific phosphorylation induces functionally active conformation in the intrinsically disordered N-terminal activation function (AF1) domain of the glucocorticoid receptor. | Molecular and cellular biology | 81 | 19841061 |
| 2010 | The hinge region of the human estrogen receptor determines functional synergy between AF-1 and AF-2 in the quantitative response to estradiol and tamoxifen. | Journal of cell science | 77 | 20332105 |
| 2000 | Characterization of transactivational property and coactivator mediation of rat mineralocorticoid receptor activation function-1 (AF-1). | Molecular endocrinology (Baltimore, Md.) | 77 | 10847590 |
| 2004 | TATA box binding protein induces structure in the recombinant glucocorticoid receptor AF1 domain. | Proceedings of the National Academy of Sciences of the United States of America | 68 | 15545613 |
| 2004 | Increases in estrogen receptor-alpha concentration in breast cancer cells promote serine 118/104/106-independent AF-1 transactivation and growth in the absence of estrogen. | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | 66 | 14718389 |
| 2004 | The relative contribution exerted by AF-1 and AF-2 transactivation functions in estrogen receptor alpha transcriptional activity depends upon the differentiation stage of the cell. | The Journal of biological chemistry | 66 | 15078875 |
| 2013 | The AF-1 activation function of estrogen receptor α is necessary and sufficient for uterine epithelial cell proliferation in vivo. | Endocrinology | 56 | 23580568 |
| 2003 | Ligand-independent coactivation of ERalpha AF-1 by steroid receptor RNA activator (SRA) via MAPK activation. | The Journal of steroid biochemistry and molecular biology | 53 | 12943696 |
| 2006 | In vivo role of the HNF4alpha AF-1 activation domain revealed by exon swapping. | The EMBO journal | 49 | 16498401 |
| 2004 | Estrogenic effects of resveratrol in breast cancer cells expressing mutant and wild-type estrogen receptors: role of AF-1 and AF-2. | The Journal of steroid biochemistry and molecular biology | 49 | 15120416 |
| 2001 | Differential contributions of AF-1 and AF-2 activities to the developmental functions of RXR alpha. | Development (Cambridge, England) | 47 | 11493527 |
| 2005 | Vinexin beta interacts with the non-phosphorylated AF-1 domain of retinoid receptor gamma (RARgamma) and represses RARgamma-mediated transcription. | The Journal of biological chemistry | 45 | 15734736 |
| 2005 | Cyclin H binding to the RARalpha activation function (AF)-2 domain directs phosphorylation of the AF-1 domain by cyclin-dependent kinase 7. | Proceedings of the National Academy of Sciences of the United States of America | 40 | 16275922 |
| 2003 | The AF-1 and AF-2 domains of RAR gamma 2 and RXR alpha cooperate for triggering the transactivation and the degradation of RAR gamma 2/RXR alpha heterodimers. | The Journal of biological chemistry | 40 | 12824162 |
| 1998 | Critical structural elements and multitarget protein interactions of the transcriptional activator AF-1 of hepatocyte nuclear factor 4. | The Journal of biological chemistry | 40 | 9792714 |
| 2012 | Binding of the N-terminal region of coactivator TIF2 to the intrinsically disordered AF1 domain of the glucocorticoid receptor is accompanied by conformational reorganizations. | The Journal of biological chemistry | 38 | 23132854 |
| 2002 | Formation of an hER alpha-COUP-TFI complex enhances hER alpha AF-1 through Ser118 phosphorylation by MAPK. | The EMBO journal | 38 | 12093745 |
| 2012 | Lessons from the dissection of the activation functions (AF-1 and AF-2) of the estrogen receptor alpha in vivo. | Steroids | 37 | 23200732 |
| 1990 | Nuclear factors AF-1 and C/EBP bind to the human ApoB gene promoter and modulate its transcriptional activity in hepatic cells. | The Journal of biological chemistry | 35 | 2161847 |
| 2000 | The AF-1 and AF-2 activating domains of retinoic acid receptor-alpha (RARalpha) and their phosphorylation are differentially involved in parietal endodermal differentiation of F9 cells and retinoid-induced expression of target genes. | Molecular endocrinology (Baltimore, Md.) | 34 | 10976918 |
| 2021 | PIWIL1 interacting RNA piR-017061 inhibits pancreatic cancer growth via regulating EFNA5. | Human cell | 31 | 33389678 |
| 2020 | MiR-645 promotes invasiveness, metastasis and tumor growth in colorectal cancer by targeting EFNA5. | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | 31 | 32036212 |
| 2013 | Variable steroid receptor responses: Intrinsically disordered AF1 is the key. | Molecular and cellular endocrinology | 31 | 23792173 |
| 2012 | Transactivating function (AF) 2-mediated AF-1 activity of estrogen receptor α is crucial to maintain male reproductive tract function. | Proceedings of the National Academy of Sciences of the United States of America | 31 | 23213263 |
| 2018 | Epha2 and Efna5 participate in lens cell pattern-formation. | Differentiation; research in biological diversity | 30 | 29800803 |
| 2018 | Oestrogen receptor α AF-1 and AF-2 domains have cell population-specific functions in the mammary epithelium. | Nature communications | 29 | 30413705 |
| 2013 | Ligand binding reduces SUMOylation of the peroxisome proliferator-activated receptor γ (PPARγ) activation function 1 (AF1) domain. | PloS one | 29 | 23826177 |
| 2004 | Fibronectin and type IV collagen activate ERalpha AF-1 by c-Src pathway: effect on breast cancer cell motility. | Oncogene | 29 | 15467744 |
| 1998 | Sézary syndrome, T-helper 2 cytokines and accessory factor-1 (AF-1). | Leukemia & lymphoma | 29 | 9613981 |
| 2011 | TBP binding-induced folding of the glucocorticoid receptor AF1 domain facilitates its interaction with steroid receptor coactivator-1. | PloS one | 28 | 21760925 |
| 2003 | Role of conserved hydrophobic amino acids in androgen receptor AF-1 function. | Journal of molecular endocrinology | 28 | 14664704 |
| 2000 | Identification and functional analysis of sequence rearrangements in the long control region of human papillomavirus type 16 Af-1 variants isolated from Ugandan penile carcinomas. | The Journal of general virology | 28 | 11086128 |
| 2013 | Tamoxifen elicits atheroprotection through estrogen receptor α AF-1 but does not accelerate reendothelialization. | The American journal of pathology | 27 | 23669343 |
| 2002 | TIF2 mediates the synergy between RARalpha 1 activation functions AF-1 and AF-2. | The Journal of biological chemistry | 27 | 12149266 |
| 2007 | Effects of different osmolytes on the induced folding of the N-terminal activation domain (AF1) of the glucocorticoid receptor. | Archives of biochemistry and biophysics | 26 | 17655821 |
| 2006 | The human androgen receptor AF1 transactivation domain: interactions with transcription factor IIF and molten-globule-like structural characteristics. | Biochemical Society transactions | 25 | 17073749 |
| 1996 | Characterization of the AB (AF-1) region in the muscle-specific retinoid X receptor-gamma: evidence that the AF-1 region functions in a cell-specific manner. | Nucleic acids research | 25 | 8628649 |
| 2021 | Root-Derived Endophytic Diazotrophic Bacteria Pantoea cypripedii AF1 and Kosakonia arachidis EF1 Promote Nitrogen Assimilation and Growth in Sugarcane. | Frontiers in microbiology | 23 | 34975800 |
| 2011 | Binding-folding induced regulation of AF1 transactivation domain of the glucocorticoid receptor by a cofactor that binds to its DNA binding domain. | PloS one | 23 | 22003412 |
| 1996 | Modulation of JunD.AP-1 DNA binding activity by AP-1-associated factor 1 (AF-1). | The Journal of biological chemistry | 23 | 8939957 |
| 2009 | Respective contribution exerted by AF-1 and AF-2 transactivation functions in estrogen receptor alpha induced transcriptional activity by isoflavones and equol: consequence on breast cancer cell proliferation. | Molecular nutrition & food research | 21 | 19065587 |
| 1997 | Identification and characterization of the AF-1 transactivation domain of thyroid hormone receptor beta1. | The Journal of biological chemistry | 21 | 9295330 |
| 2006 | Estrogen receptor isoform-specific regulation of the retinoblastoma-binding protein 1 (RBBP1) gene: roles of AF1 and enhancer elements. | The Journal of biological chemistry | 19 | 16873370 |
| 2018 | N-terminal transactivation function, AF-1, of estrogen receptor alpha controls obesity through enhancement of energy expenditure. | Molecular metabolism | 18 | 30287090 |
| 1996 | Metabolism of AF1 (KNEFIRF-NH2) in the nematode, Ascaris suum, by aminopeptidase, endopeptidase and deamidase enzymes. | Molecular and biochemical parasitology | 18 | 8992314 |
| 1986 | The effects of polyinosinic:polycytidylic acid (pI:C) on the graft-vs-host (GVH) reaction. II. Increased NK-mediated rejection on C57BL/6 lymphocytes by (C57BL/6 X A)F1 mice. | Journal of immunology (Baltimore, Md. : 1950) | 17 | 3537120 |
| 1997 | LERK-7: a ligand of the Eph-related kinases is developmentally regulated in the brain. | Cytokine | 16 | 9245480 |
| 2010 | Comparative effects of R- and S-equol and implication of transactivation functions (AF-1 and AF-2) in estrogen receptor-induced transcriptional activity. | Nutrients | 15 | 22254026 |
| 2000 | Overexpression of mitogen-activated protein kinase superfamily proteins unrelated to Ras and AF-1 of estrogen receptor alpha mutation in advanced stage human breast cancer. | Pathology, research and practice | 15 | 11156322 |
| 2018 | Mapping of breakpoints in balanced chromosomal translocations by shallow whole-genome sequencing points to EFNA5, BAHD1 and PPP2R5E as novel candidates for genes causing human Mendelian disorders. | Journal of medical genetics | 14 | 30352868 |
| 2014 | Mutational screening of EFNA5 in Chinese age-related cataract patients. | Ophthalmic research | 14 | 25300504 |
| 2010 | Influence of flanking sequences on signaling between the activation function AF1 and DNA-binding domain of the glucocorticoid receptor. | Archives of biochemistry and biophysics | 14 | 20188692 |
| 1998 | Polyketomycin, a new antibiotic from Streptomyces sp. MK277-AF1. I. Taxonomy, production, isolation, physico-chemical properties and biological activities. | The Journal of antibiotics | 14 | 9531983 |
| 2013 | Laccase production optimization by response surface methodology with Aspergillus fumigatus AF1 in unique inexpensive medium and decolorization of different dyes with the crude enzyme or fungal pellets. | Journal of hazardous materials | 13 | 24140539 |
| 2011 | A specific antibody to neuropeptide AF1 (KNEFIRFamide) recognizes a small subset of neurons in Ascaris suum: differences from Caenorhabditis elegans. | The Journal of comparative neurology | 13 | 21452223 |
| 1997 | Purification and properties of a membrane aminopeptidase from Ascaris suum muscle that degrades neuropeptides AF1 and AF2. | Molecular and biochemical parasitology | 12 | 9364967 |
| 1990 | Progenitor cells in murine bone marrow stimulated by growth factors produced by the AF1-19T rat cell line. | Experimental hematology | 11 | 2186922 |
| 2007 | Loss of E-cadherin-mediated cell contacts reduces estrogen receptor alpha (ER alpha) transcriptional efficiency by affecting the respective contribution exerted by AF1 and AF2 transactivation functions. | Biochemical and biophysical research communications | 9 | 17991426 |
| 2023 | Overexpression of LINC00607 inhibits cell growth and aggressiveness by regulating the miR-1289/EFNA5 axis in non-small-cell lung cancer. | Open medicine (Warsaw, Poland) | 8 | 37333453 |
| 2020 | The tissue-specific effects of different 17β-estradiol doses reveal the key sensitizing role of AF1 domain in ERα activity. | Molecular and cellular endocrinology | 8 | 32004676 |
| 2020 | A new compound targets the AF-1 of androgen receptor and decreases its activity and protein levels in prostate cancer cells. | American journal of cancer research | 8 | 33415022 |
| 2019 | Assays to Interrogate the Ability of Compounds to Inhibit the AF-2 or AF-1 Transactivation Domains of the Androgen Receptor. | Assay and drug development technologies | 7 | 31502857 |
| 2007 | Non-steroidal antiandrogens act as AF-1 agonists under conditions of high androgen-receptor expression. | The Prostate | 7 | 17342748 |
| 1996 | The gene encoding LERK-7 (EPLG7, Epl7), a ligand for the Eph-related receptor tyrosine kinases, maps to human chromosome 5 at band q21 and to mouse chromosome 17. | Genomics | 7 | 8661153 |
| 2010 | Mapping of AF1 transactivation domains in duplicated rainbow trout glucocorticoid receptors. | Journal of molecular endocrinology | 6 | 20921243 |
| 2004 | Prenylation inhibitors stimulate both estrogen receptor alpha transcriptional activity through AF-1 and AF-2 and estrogen receptor beta transcriptional activity. | Breast cancer research : BCR | 6 | 15642170 |
| 2001 | Deletion of RAR carboxyl terminus reveals promoter- and receptor-specific AF-1 effects. | Biochemical and biophysical research communications | 6 | 11741282 |
| 2016 | Expression of mammalian mitochondrial F1-ATPase in Escherichia coli depends on two chaperone factors, AF1 and AF2. | FEBS open bio | 5 | 28203526 |
| 1981 | Influence of growth temperature on structure, thermostability and kinetic properties of the ATPase coupling factor AF1 of a thermophilic blue-green alga (cyanobacterium). | European journal of biochemistry | 5 | 6456904 |
| 2024 | Five New Indole Alkaloid Derivatives from Deep-Sea Fungus Aspergillus fumigatus AF1. | Marine drugs | 4 | 39852506 |
| 2023 | Transcription Factor EGR1 Facilitates Neovascularization in Mice with Retinopathy of Prematurity by Regulating the miR-182-5p/EFNA5 Axis. | Biochemical genetics | 4 | 37530910 |
| 2004 | In vitro regulation of reporter gene transcription by the androgen receptor AF1 domain. | Biochemical Society transactions | 4 | 15506979 |
| 2025 | An Alternatively Translated Isoform of PPARG Suggests AF-1 Domain Inhibition as an Insulin Sensitization Target. | Diabetes | 2 | 39854214 |
| 2024 | EFNA5 suppresses cell proliferation and tumor metastasis in hepatoma via epithelial-to-mesenchymal transition. | Discover oncology | 2 | 39424684 |
| 2024 | The N-terminal activation function AF-1 domain of ERα interacts directly with the C-terminal AF-2-holding ligand-binding domain to recruit the coactivator proteins. | PloS one | 2 | 39432505 |
| 2008 | A novel poly(3-hydroxybutyrate)-degrading Streptoverticillium kashmirense AF1 isolated from soil and purification of PHB-depolymerase. | Acta biologica Hungarica | 2 | 19133504 |
| 2025 | EFNA5 as an Oocyte-Derived Factor Enhances Developmental Competence by Modulating Oxidative Stress, Inflammation, and Apoptosis During In Vitro Maturation. | Antioxidants (Basel, Switzerland) | 1 | 41462676 |
| 2014 | AF-1 a novel regulator of the redox equilibrium during aging. | Free radical biology & medicine | 1 | 26461309 |
| 2013 | The Transactivating Function 2 (AF-2) of Estrogen Receptor (ER) α is Indispensable for ERα-mediated Physiological Responses and AF-1 Activity. | Open journal of endocrine and metabolic diseases | 1 | 30310730 |
| 2009 | Finding Alu in primate genomes with AF-1. | Bioinformation | 1 | 19293991 |
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