DNAJC14
DnaJ homolog subfamily C member 14 · UniProt Q6Y2X3
- Length
- 702 aa
- Mass
- 78.6 kDa
- Annotated
- 2026-04-28
100 papers in source corpus
4 papers cited in narrative
4 extracted findings
Mechanistic narrative
Synthesis pass · prose summary of the discoveries below
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Mechanism profile
Synthesis pass · controlled-vocabulary classification · explore literature graph →
No controlled-vocabulary terms were assigned to this entry.
Evidence
Reading pass · 4 per-paper findings extracted from the source corpus
| Year | Finding | Method | Journal | Conf | PMIDs |
|---|---|---|---|---|---|
| 2011 | DNAJC14 is a mammalian Hsp40 chaperone that, when overexpressed, inhibits yellow fever virus (YFV) replication at the step of viral RNA replication. Both the J-domain and the C-terminal self-interaction domain are required for anti-YFV activity. Endogenous DNAJC14 redistributes to viral replication complexes (identified by dsRNA staining) during infection, and silencing of endogenous DNAJC14 impairs YFV replication, indicating a requirement for DNAJC14 in replication complex assembly. The antiviral effect of overexpressed DNAJC14 is stoichiometric — excess DNAJC14 disrupts proper assembly, which can be overcome as viral nonstructural proteins accumulate. | cDNA library screen, siRNA knockdown, overexpression with mutagenesis, immunofluorescence co-localization with dsRNA, antiviral activity assays across multiple Flaviviridae members | PLoS pathogens | High | 21249176 |
| 2012 | DNAJC14 redistributes and clusters with YFV nonstructural proteins via a transmembrane domain and a newly identified membrane-binding domain (MBD), both of which mediate targeting to detergent-resistant membranes. The C-terminal self-interaction domain is required for entry into the viral replication complex (RC) protein interaction network that persists after 1% Triton solubilization. Fusion of the DNAJC14 MBD and self-interaction domain onto another Hsp40 is sufficient to confer YFV-inhibitory activity, supporting a model in which DNAJC14 acts as a membrane-targeted chaperone scaffold that mediates RC formation. | Mutagenesis of DNAJC14 domains, detergent-resistant membrane fractionation, co-immunoprecipitation, domain-fusion rescue experiments | Journal of virology | High | 22915803 |
| 2016 | DNAJC14 and Hsc70 are required for unconventional (Golgi-independent) cell-surface trafficking of misfolded H723R-pendrin (encoded by SLC26A4). Blockade of ER-to-Golgi transport or ER stress activation induced Golgi-independent surface expression of H723R-pendrin, restoring its Cl⁻/HCO₃⁻ exchange activity. Proteomic and siRNA screening identified Hsc70 and DNAJC14 as essential factors; DNAJC14 upregulation alone was sufficient to induce unconventional surface expression of H723R-pendrin. | Proteomic screening, siRNA knockdown, pharmacological blockade of ER-to-Golgi transport, functional ion-transport assay, overexpression rescue | Nature communications | High | 27109633 |
| 2019 | CRISPR/Cas9 knockout of DNAJC14 in bovine and porcine cells demonstrated that DNAJC14 is an essential cellular cofactor specifically for RNA replication of noncytopathogenic (noncp) pestiviruses (6 distinct species: A–D, F, G), but not for cytopathogenic (cp) pestiviruses. The pestiviral replicase NS3-5B can assemble into functional complexes in the absence of DNAJC14, indicating DNAJC14's role is specifically in NS2 autoprotease activation (which releases NS3) rather than in downstream replicase assembly. | CRISPR/Cas9 knockout of DNAJC14, viral replication assays across multiple pestivirus species, complementation experiments | Journal of virology | High | 30518653 |
Source papers
Stage 0 corpus · 100 papers · ranked by NIH iCite citations
| Year | Title | Journal | Citations | PMID |
|---|---|---|---|---|
| 1991 | Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK. | Proceedings of the National Academy of Sciences of the United States of America | 744 | 1826368 |
| 2006 | The diversity of the DnaJ/Hsp40 family, the crucial partners for Hsp70 chaperones. | Cellular and molecular life sciences : CMLS | 684 | 16952052 |
| 1993 | DnaK, DnaJ and GrpE form a cellular chaperone machinery capable of repairing heat-induced protein damage. | The EMBO journal | 531 | 7900997 |
| 1994 | DnaJ-like proteins: molecular chaperones and specific regulators of Hsp70. | Trends in biochemical sciences | 409 | 8016869 |
| 1998 | Chaperone coexpression plasmids: differential and synergistic roles of DnaK-DnaJ-GrpE and GroEL-GroES in assisting folding of an allergen of Japanese cedar pollen, Cryj2, in Escherichia coli. | Applied and environmental microbiology | 346 | 9572938 |
| 2004 | hsp70-DnaJ chaperone pair prevents nitric oxide- and CHOP-induced apoptosis by inhibiting translocation of Bax to mitochondria. | Cell death and differentiation | 302 | 14752510 |
| 2008 | Structural and functional diversities between members of the human HSPB, HSPH, HSPA, and DNAJ chaperone families. | Biochemistry | 291 | 18557634 |
| 1999 | ClpB cooperates with DnaK, DnaJ, and GrpE in suppressing protein aggregation. A novel multi-chaperone system from Escherichia coli. | The Journal of biological chemistry | 290 | 10497158 |
| 1996 | A cycle of binding and release of the DnaK, DnaJ and GrpE chaperones regulates activity of the Escherichia coli heat shock transcription factor sigma32. | The EMBO journal | 232 | 8599944 |
| 2001 | Its substrate specificity characterizes the DnaJ co-chaperone as a scanning factor for the DnaK chaperone. | The EMBO journal | 225 | 11230128 |
| 1991 | Function of DnaJ and DnaK as chaperones in origin-specific DNA binding by RepA. | Nature | 200 | 2005967 |
| 1988 | Role of the Escherichia coli DnaK and DnaJ heat shock proteins in the initiation of bacteriophage lambda DNA replication. | Proceedings of the National Academy of Sciences of the United States of America | 197 | 2970643 |
| 1992 | DnaK and DnaJ heat shock proteins participate in protein export in Escherichia coli. | Genes & development | 194 | 1628824 |
| 1992 | Cooperation of GroEL/GroES and DnaK/DnaJ heat shock proteins in preventing protein misfolding in Escherichia coli. | Proceedings of the National Academy of Sciences of the United States of America | 189 | 1359538 |
| 1985 | Purification and properties of the dnaJ replication protein of Escherichia coli. | The Journal of biological chemistry | 169 | 3889001 |
| 1993 | Control of folding and membrane translocation by binding of the chaperone DnaJ to nascent polypeptides. | Proceedings of the National Academy of Sciences of the United States of America | 158 | 8234279 |
| 1995 | Positive selection in autoimmunity: abnormal immune responses to a bacterial dnaJ antigenic determinant in patients with early rheumatoid arthritis. | Nature medicine | 150 | 7585093 |
| 1996 | Cooperative action of Hsp70, Hsp90, and DnaJ proteins in protein renaturation. | Biochemistry | 144 | 8942653 |
| 1992 | DnaK, DnaJ, and GrpE are required for flagellum synthesis in Escherichia coli. | Journal of bacteriology | 141 | 1400176 |
| 1998 | Zuotin, a ribosome-associated DnaJ molecular chaperone. | The EMBO journal | 138 | 9707440 |
| 1992 | MAS5, a yeast homolog of DnaJ involved in mitochondrial protein import. | Molecular and cellular biology | 137 | 1729605 |
| 1993 | The yeast SIS1 protein, a DnaJ homolog, is required for the initiation of translation. | Cell | 129 | 8513501 |
| 1993 | Genetic interactions between KAR2 and SEC63, encoding eukaryotic homologues of DnaK and DnaJ in the endoplasmic reticulum. | Molecular biology of the cell | 125 | 8305736 |
| 2013 | A chloroplast-targeted DnaJ protein contributes to maintenance of photosystem II under chilling stress. | Journal of experimental botany | 123 | 24227338 |
| 1995 | Modulation of the ATPase activity of the molecular chaperone DnaK by peptides and the DnaJ and GrpE heat shock proteins. | The Journal of biological chemistry | 123 | 7876226 |
| 2018 | DNAJ Proteins in neurodegeneration: essential and protective factors. | Philosophical transactions of the Royal Society of London. Series B, Biological sciences | 113 | 29203718 |
| 2000 | Human DnaJ homologs dj2 and dj3, and bag-1 are positive cochaperones of hsc70. | The Journal of biological chemistry | 113 | 10816573 |
| 2000 | Mammalian HSP40/DNAJ homologs: cloning of novel cDNAs and a proposal for their classification and nomenclature. | Cell stress & chaperones | 113 | 11147971 |
| 1991 | A homologue of the bacterial heat-shock gene DnaJ that alters protein sorting in yeast. | Nature | 109 | 2000136 |
| 1993 | Cloning, nucleotide sequence, and regulatory analysis of the Lactococcus lactis dnaJ gene. | Journal of bacteriology | 105 | 8449872 |
| 1996 | Mechanism of clathrin basket dissociation: separate functions of protein domains of the DnaJ homologue auxilin. | The Journal of cell biology | 102 | 8922377 |
| 1994 | An analogue of the DnaJ molecular chaperone in Escherichia coli. | Proceedings of the National Academy of Sciences of the United States of America | 101 | 8302830 |
| 1997 | The human DnaJ homologue dj2 facilitates mitochondrial protein import and luciferase refolding. | The Journal of cell biology | 100 | 9382858 |
| 2005 | The DnaJ-domain protein RME-8 functions in endosomal trafficking. | The Journal of biological chemistry | 98 | 16179350 |
| 2017 | DnaJ Heat Shock Protein Family B Member 9 Is a Novel Biomarker for Fibrillary GN. | Journal of the American Society of Nephrology : JASN | 94 | 29097623 |
| 2007 | dnaJ gene sequence-based assay for species identification and phylogenetic grouping in the genus Staphylococcus. | International journal of systematic and evolutionary microbiology | 93 | 17220435 |
| 1990 | Isolation and characterization of dnaJ null mutants of Escherichia coli. | Journal of bacteriology | 92 | 2144273 |
| 2011 | Human DNAJ in cancer and stem cells. | Cancer letters | 91 | 21925790 |
| 1998 | A novel human DnaJ protein, hTid-1, a homolog of the Drosophila tumor suppressor protein Tid56, can interact with the human papillomavirus type 16 E7 oncoprotein. | Virology | 87 | 9683573 |
| 2000 | Solution structure of the cysteine-rich domain of the Escherichia coli chaperone protein DnaJ. | Journal of molecular biology | 85 | 10891270 |
| 2013 | Overexpression of a soybean nuclear localized type-III DnaJ domain-containing HSP40 reveals its roles in cell death and disease resistance. | The Plant journal : for cell and molecular biology | 80 | 23289813 |
| 2000 | Identification of Mrj, a DnaJ/Hsp40 family protein, as a keratin 8/18 filament regulatory protein. | The Journal of biological chemistry | 80 | 10954706 |
| 2018 | Novel DNAJ-related proteins in Arabidopsis thaliana. | The New phytologist | 78 | 29271039 |
| 2001 | Loss of expression of a new member of the DNAJ protein family confers resistance to chemotherapeutic agents used in the treatment of ovarian cancer. | Cancer research | 78 | 11358853 |
| 1998 | A role for the DnaJ homologue Scj1p in protein folding in the yeast endoplasmic reticulum. | The Journal of cell biology | 76 | 9817751 |
| 1992 | DnaJ, DnaK, and GrpE heat shock proteins are required in oriP1 DNA replication solely at the RepA monomerization step. | Proceedings of the National Academy of Sciences of the United States of America | 76 | 1438220 |
| 1997 | The yeast JEM1p is a DnaJ-like protein of the endoplasmic reticulum membrane required for nuclear fusion. | The Journal of biological chemistry | 75 | 9148890 |
| 2015 | A tomato chloroplast-targeted DnaJ protein protects Rubisco activity under heat stress. | Journal of experimental botany | 74 | 25801077 |
| 1997 | HSDJ, a human homolog of DnaJ, is farnesylated and is involved in protein import into mitochondria. | Journal of biochemistry | 71 | 9192730 |
| 2012 | ERdj4 protein is a soluble endoplasmic reticulum (ER) DnaJ family protein that interacts with ER-associated degradation machinery. | The Journal of biological chemistry | 70 | 22267725 |
| 2007 | Phylogeny and species identification of the family Enterobacteriaceae based on dnaJ sequences. | Diagnostic microbiology and infectious disease | 66 | 17368802 |
| 1997 | The DnaJ-like cysteine string protein and exocytotic neurotransmitter release. | Trends in neurosciences | 66 | 9141199 |
| 2011 | Identification and characterization of the host protein DNAJC14 as a broadly active flavivirus replication modulator. | PLoS pathogens | 65 | 21249176 |
| 2005 | A type I DnaJ homolog, DjA1, regulates androgen receptor signaling and spermatogenesis. | The EMBO journal | 65 | 15660130 |
| 2005 | Defense against protein carbonylation by DnaK/DnaJ and proteases of the heat shock regulon. | Journal of bacteriology | 65 | 15937182 |
| 2003 | Mutation of host DnaJ homolog inhibits brome mosaic virus negative-strand RNA synthesis. | Journal of virology | 65 | 12584324 |
| 1998 | The cytosolic DnaJ-like protein djp1p is involved specifically in peroxisomal protein import. | The Journal of cell biology | 65 | 9679141 |
| 2013 | A novel mitochondrial DnaJ/Hsp40 family protein BIL2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling. | Planta | 64 | 23494613 |
| 2007 | Trigger Factor can antagonize both SecB and DnaK/DnaJ chaperone functions in Escherichia coli. | Proceedings of the National Academy of Sciences of the United States of America | 64 | 17360615 |
| 2010 | Role of DnaJ G/F-rich domain in conformational recognition and binding of protein substrates. | The Journal of biological chemistry | 61 | 20729526 |
| 2003 | DnaK and DnaJ facilitated the folding process and reduced inclusion body formation of magnesium transporter CorA overexpressed in Escherichia coli. | Protein expression and purification | 60 | 14965767 |
| 1993 | Human cDNA encoding DnaJ protein homologue. | Biochimica et biophysica acta | 60 | 8334161 |
| 1995 | A novel function of Escherichia coli chaperone DnaJ. Protein-disulfide isomerase. | The Journal of biological chemistry | 58 | 7559385 |
| 2016 | The HSP70 co-chaperone DNAJC14 targets misfolded pendrin for unconventional protein secretion. | Nature communications | 56 | 27109633 |
| 2014 | LeCDJ1, a chloroplast DnaJ protein, facilitates heat tolerance in transgenic tomatoes. | Journal of integrative plant biology | 56 | 24148796 |
| 1980 | Identification of the E. coli dnaJ gene product. | Molecular & general genetics : MGG | 56 | 6446654 |
| 1995 | Interaction between hsp70 and hsp40, eukaryotic homologues of DnaK and DnaJ, in human cells expressing mutant-type p53. | FEBS letters | 53 | 7828728 |
| 1993 | Isolation of dnaJ, dnaK, and grpE homologues from Borrelia burgdorferi and complementation of Escherichia coli mutants. | Molecular microbiology | 53 | 8459764 |
| 1993 | Heat shock proteins DnaJ, DnaK, and GrpE stimulate P1 plasmid replication by promoting initiator binding to the origin. | Journal of bacteriology | 53 | 8501058 |
| 2019 | An acquired scaffolding function of the DNAJ-PKAc fusion contributes to oncogenic signaling in fibrolamellar carcinoma. | eLife | 52 | 31063128 |
| 2014 | Structure and function of human DnaJ homologue subfamily a member 1 (DNAJA1) and its relationship to pancreatic cancer. | Biochemistry | 51 | 24512202 |
| 2001 | hTid-1, a human DnaJ protein, modulates the interferon signaling pathway. | The Journal of biological chemistry | 51 | 11679576 |
| 1996 | A novel DnaJ-like protein in Escherichia coli inserts into the cytoplasmic membrane with a type III topology. | Molecular microbiology | 51 | 8809778 |
| 2008 | Ribosome biogenesis is temperature-dependent and delayed in Escherichia coli lacking the chaperones DnaK or DnaJ. | Molecular microbiology | 48 | 19054328 |
| 2015 | The deubiquitinase ataxin-3 requires Rad23 and DnaJ-1 for its neuroprotective role in Drosophila melanogaster. | Neurobiology of disease | 46 | 26007638 |
| 2006 | Tobacco Tsip1, a DnaJ-type Zn finger protein, is recruited to and potentiates Tsi1-mediated transcriptional activation. | The Plant cell | 45 | 16844903 |
| 2019 | Novel DnaJ Protein Facilitates Thermotolerance of Transgenic Tomatoes. | International journal of molecular sciences | 44 | 30654548 |
| 2017 | The DnaJ protein OsDjA6 negatively regulates rice innate immunity to the blast fungus Magnaporthe oryzae. | Molecular plant pathology | 43 | 28220688 |
| 2018 | DnaJ/Hsp40 Family and Parkinson's Disease. | Frontiers in neuroscience | 42 | 29367843 |
| 2012 | Flavivirus replication complex assembly revealed by DNAJC14 functional mapping. | Journal of virology | 41 | 22915803 |
| 2007 | DnaJ recruits DnaK to protein aggregates. | The Journal of biological chemistry | 41 | 17984091 |
| 2004 | CbpA, a DnaJ homolog, is a DnaK co-chaperone, and its activity is modulated by CbpM. | The Journal of biological chemistry | 40 | 15184371 |
| 2018 | DksA-DnaJ redox interactions provide a signal for the activation of bacterial RNA polymerase. | Proceedings of the National Academy of Sciences of the United States of America | 39 | 30429329 |
| 2001 | A DnaJ protein, apobec-1-binding protein-2, modulates apolipoprotein B mRNA editing. | The Journal of biological chemistry | 39 | 11584023 |
| 2006 | Tuning of DnaK chaperone action by nonnative protein sensor DnaJ and thermosensor GrpE. | The Journal of biological chemistry | 37 | 16940296 |
| 2012 | DNAJ proteins and protein aggregation diseases. | Current topics in medicinal chemistry | 36 | 23339302 |
| 2007 | The dnaJ gene as a novel phylogenetic marker for identification of Vibrio species. | Systematic and applied microbiology | 36 | 17207598 |
| 2004 | Characterization of pancreatic ERj3p, a homolog of yeast DnaJ-like protein Scj1p. | Biological chemistry | 34 | 15195998 |
| 2022 | Differential roles for DNAJ isoforms in HTT-polyQ and FUS aggregation modulation revealed by chaperone screens. | Nature communications | 32 | 35082301 |
| 2016 | The DnaJ-Like Zinc Finger Domain Protein PSA2 Affects Light Acclimation and Chloroplast Development in Arabidopsis thaliana. | Frontiers in plant science | 31 | 27047527 |
| 2017 | Overexpression of DnaJ-Like Chaperone Enhances Carotenoid Synthesis in Chlamydomonas reinhardtii. | Applied biochemistry and biotechnology | 30 | 28612271 |
| 2009 | Pc4, a putative movement protein of Rice stripe virus, interacts with a type I DnaJ protein and a small Hsp of rice. | Virus genes | 30 | 19130198 |
| 2001 | Zinc fingers and thiol-disulfide oxidoreductase activities of chaperone DnaJ. | Biochemistry | 30 | 11732919 |
| 2019 | CRISPR/Cas9-Mediated Knockout of DNAJC14 Verifies This Chaperone as a Pivotal Host Factor for RNA Replication of Pestiviruses. | Journal of virology | 29 | 30518653 |
| 2013 | Combining crystallography and EPR: crystal and solution structures of the multidomain cochaperone DnaJ. | Acta crystallographica. Section D, Biological crystallography | 29 | 23897477 |
| 2004 | The Escherichia coli DjlA and CbpA proteins can substitute for DnaJ in DnaK-mediated protein disaggregation. | Journal of bacteriology | 28 | 15489435 |
| 1991 | Cloning of several lignin peroxidase (LIP)-encoding genes: sequence analysis of the LIP6 gene from the white-rot basidiomycete, Phanerochaete chrysosporium. | Gene | 28 | 1999283 |
| 2010 | Lon protease quality control of presecretory proteins in Escherichia coli and its dependence on the SecB and DnaJ (Hsp40) chaperones. | The Journal of biological chemistry | 26 | 20504766 |
| 2020 | Inhibition of DNAJ-HSP70 interaction improves strength in muscular dystrophy. | The Journal of clinical investigation | 25 | 32427588 |
| 2007 | Mycobacterium species identification--a new approach via dnaJ gene sequencing. | Systematic and applied microbiology | 25 | 17640840 |